With our marine ecosystems under threat from climate change, there is an urgent need to continuously monitor marine conditions. One key indicator is the dissolved oxygen level, but existing sensors are limited by size and costs that preclude widespread non-intrusive monitoring. This work reports a new dual-gate design based on organic electrochemical transistors (OECTs) to track dissolved oxygen concentration in seawater, a highly challenging matrix owing to its high ionic strength and multitude of chemical interferents. We present the novel operating principle, by deriving the channel conductance with respect to potentials on the two gates. The dual-gate OECT was highly sensitive and stable compared to the conventional OECT structure, as the dual-gate configuration extended the device stability window by preventing undesirable reactions at the OECT channel. Specifically, the sensor achieved a detection limit of 0.5 ppm dissolved oxygen concentration in seawater. The device demonstrated reliable operation over five days and was capable of monitoring oxygenation changes arising from the photosynthesis cycles of saltwater macro-algae. In addition, we also engineer a system to monitor the correlation of oyster movement with dissolved oxygen in its environment, and it offers a new design to realize compact, highly sensitive, economical in-situ sensors for harsh marine environments.