Dual-channel Detection Research Articles

A bifunctional fluorescent probe for dual-channel detection of H2O2 and HOCl in living cells

Hydrogen peroxide (H2O2) and hypochlorous acid (HOCl) are critical reactive oxygen species (ROS) that play significant roles in regulating oxidative stress, closely tied to various human diseases. However, investigating their interplay within living cells has been challenging due to the lack of effective tools for simultaneous discrimination. Herein, we present a bifunctional fluorescent probe, PTZ-H-H, capable of simultaneously detecting H2O2 and HOCl in living cells via two distinct fluorescence channels. PTZ-H-H exhibits selective and sensitive responses, emitting red fluorescence in the presence of H2O2 and green fluorescence in response to HOCl, with detection limits of 386 nM and 16.8 nM, respectively. The probe was successfully applied in living cells, enabling real-time monitoring of intracellular H2O2 and HOCl. This study demonstrates the potential of PTZ-H-H as a powerful tool for exploring the dynamic roles of H2O2 and HOCl in various physiological and pathological processes.

Development of a MOF-based dual-channel levofloxacin probe and its application in the detection of food and beverage

Food safety issues are becoming increasingly prominent, raising public concern. Antibiotic pollutants, as a unique category, act like a double-edged sword. On one hand, they provide precise treatment for related diseases, but on the other hand, their excessive use or improper handling can lead to environmental contamination or entry into the biological chain. This makes it easy for living organisms to come into contact with and ingest these pollutants, leading to serious consequences. Among these antibiotics, Levofloxacin (LVF), a broad-spectrum antibacterial agent, effectively treats various bacterial infections but also causes severe problems due to its misuse. Therefore, developing a simple, fast, and efficient probe for detecting LVF is crucial for environmental protection and human safety. In this study, a novel UiO-66-F4 fluorescent probe was synthesized. This probe demonstrated dual-channel detection of LVF through its inner filter effect (IFE) and chemical interactions, resulting in a visible color change from colorless to green. It exhibits good linear ranges at the fluorescence peaks of 494 nm (30–68 μM), with a detection limit of 0.1 μM. Based on its excellent detection performance, the probe was further applied for the quantitative determination of LVF in different types of food and beverages samples, yielding satisfactory results with recovery rates ranging from 93.4 % to 114.6 %. This probe offers a reliable means for the detection and evaluation of trace amounts of LVF in food.

CeO2 loaded on L-tryptophan functionalized graphitic carbon nitride colorimetric-fluorescent dual-channel detection

In this study, a nanocomposite with CeO2 loaded on L-tryptophan functionalized graphitic carbon nitride (CeO2/L-g-C3N4) was developed by a facile two-step method. Firstly, L-tryptophan functionalized graphitic carbon nitride (L-g-C3N4) was achieved via π-π stacking interactions through a physical ultrasonic treatment process, which had better dispersibility in water than Bulk-g-C3N4. Then the final product of CeO2/L-g-C3N4 was obtained by a hydrothermal co-precipitation technique with cerium nitrate as precursor. The characterization of morphology indicated that CeO2 nanoparticles evenly distributed on the surface of CeO2/L-g-C3N4, which might be attributed to L-tryptophan offering more bonding sites (–COOH) for the deposition of nanoparticles. The material exhibited excellent oxidase-mimicking activity, which could catalyze the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into the blue-colored oxidized TMB product (oxTMB), and a new hydroquinone (HQ) colorimetric sensing strategy was proposed. And theoretical simulations indicate that the increased enzymatic activity is mainly attributable to the reduced binding energy of absorbed intermediates. Additionally, due to the material’s exceptional fluorescence properties, combined with the inner filter effect and redox reactions, a unique on–off-on fluorescence sensing mechanism had been successfully developed for the detection of Cr(VI) and SO32−.

Advanced dual-channel sensing of coumarin with phosphorescent carbon dots: Mechanism insights

Room-temperature phosphorescence carbon dots (CDs) show the highlighted advantages in chemical sensing and bioimaging owing to its long lifetime, however, acquiring the efficient phosphorescence of carbon dots is still encountering difficulty due to the spin prohibition. Here, we synthesized one type of CDs through one-step hydrothermal method. To be specific, the CDs displayed the blue fluorescence in aqueous, and emitted the bright green phosphorescence when being coated on the filter paper. Importantly, introducing coumarin to the CDs resulted in the distinct quenching of both its fluorescence and phosphorescence. Based on this phenomenon, we successfully established a dual-channel detection of coumarin. The fluorescence detection channel exhibited a linear range of 5–350 μM with a detection limit of 0.196 μM, while the phosphorescence detection channel achieved a linear range of 50–1000 μM with a detection limit of 0.268 μM. The further investigation revealed that IFE and SQE were mainly responsible for the fluorescence quenching of CDs, while the phosphorescence quenching was originated from the increased energy gap between the singlet and triplet states (ΔEST) of CDs, thus obstructing the ISC process. Moreover, we employed the CDs as the phosphorescent ink for anti-counterfeiting.

Near-infrared multi-functional aggregation-induced emission fluorescent probe for detection of viscosity and peroxynitrite and its imaging application

Viscosity and peroxynitrite (ONOO−) are two crucial elements that influence the operational status of biological processes within living organisms. Their abnormal changes cause metabolic disorders and even diseases. Therefore, it is important to develop a near-infrared (NIR) probe with aggregation-induced emission (AIE) feature that is capable of dual channel detection of both viscosity and ONOO−. Here, a new NIR fluorescent probe LY with large Stokes shift was synthesized in which triphenylamine was conjugated to a pyridinium phenylborate salt. Probe LY with the distorted intramolecular twisted intramolecular charge transfer (TICT) properties is sensitive to systemic viscosity and exhibits enhanced fluorescence emission with peak at the 704 nm. On the other hand, the probe is also susceptible to peroxynitrite-triggered borate oxidation, providing an AIE fluorescence-enhanced response at the 639 nm peak. Moreover, with excellent photochemical stability, lower cytotoxicity and a precise targeting ability for mitochondria, probe LY was used to visualize imaging changes in endogenous and exogenous cellular viscosity and peroxynitrite. The imaging results of the probe during ferroptosis confirmed that both the intracellular viscosity microenvironment and peroxynitrite were further overexpressed. Finally, in vivo imaging experiments showed that the occurrence of inflammation was accompanied by an increase in the viscosity and the release in peroxynitrite.

Utilization of Te as a saturable absorber for mode-locked 2.8 µm Er3+: ZBLAN fiber laser

In this manuscript, we present the experimental realization of a mode-locked Er3+: ZBLAN (ZrF2- BaF2- LaF3- AlF3- NaF) fiber laser operating at mid-infrared (mid-IR) 2.8 μm band, achieved through the utilization of Tellurium (Te) as the saturable absorber (SA). An exploration of their nonlinear optical absorption properties at 2.8 μm was undertaken employing a dual-channel detection methodology. Our investigation reveals that the Te-SA prepared exhibits a significant broadband saturable absorption response, thereby confirming its suitability as SA for passive mode-locked fiber laser at 2.8 μm. The peak average output power pulse and energy of the mode-locked Er3+: ZBLAN fiber laser are 41.2mW and 1.632nJ, respectively. These measurements were conducted at a repetition rate of 25.25 MHz, accompanied by a signal-to-noise ratio (SNR) of 60 dB at a transmit pump power of 600 mW. This is the first demonstration of a mode-locked fiber laser operating in the 2.8 µm mid infrared band using Te-SA.

A dual-channel fluorescent probe targeting lysosomes for differential detection of Cys/Hcy and GSH: Applications in food, pharmaceutical analysis and bioimaging

Thiols function as antioxidants in food, prolonging shelf life and enhancing flavor. Moreover, thiols are vital biomolecules involved in enzyme activity, cellular signal transduction, and protein folding among critical biological processes. In this paper, the fluorescent probe PYL-NBD was designed and synthesized, which utilized the fluorescent molecule pyrazoline, the lysosome-targeted morpholine moiety, and the sensing moiety NBD. Probe PYL-NBD was tailored for the recognition of biothiols through single-wavelength excitation, yielding distinct fluorescence emission signals: blue for Cys, Hcy, and GSH; green for Cys, Hcy. Probe PYL-NBD exhibited rapid reaction kinetics (<10 min), distinct fluorescence response signals, and low detection limits (15.7 nM for Cys, 14.4 nM for Hcy, and 12.6 nM for GSH). Probe PYL-NBD enabled quantitative determination of Cys content in food samples and L-cysteine capsules. Furthermore, probe PYL-NBD had been successfully applied for confocal imaging with dual-channel detection of biothiols in various biological specimens, including HeLa cells, zebrafish, tumor sections, and Arabidopsis thaliana.

Fluorescence switch based on Prussian blue nanoparticles and CdSe quantum dots for dual-channel detection of hydrazine hydrate and hydrogen peroxide

The detection of hydrazine hydrate and hydrogen peroxide is becoming increasingly important since they are of significance in industrial production and diverse applications in several fields. In this work, a Prussian blue (PB) and CdSe quantum dots based fluorescent switch PB@SiO2/CdSe@SiO2 is constructed, which is developed as a platform towards the detection of hydrazine and hydrogen peroxide. The nanocomposite can realize the switching between fluorescence quenched and restored states by transforming PB between oxidized and reduced states by redox reactions. Fluorescence spectroscopy and UV–vis absorption spectroscopy are employed to detect N2H4 and H2O2 with low detection limits, wide detection ranges and short response time. The proposed preparation method may also be useful for the assembly of other quantum dot nanocomposites, and these properties make PB@SiO2/CdSe@SiO2 potentially applicable in the field of environmental detection.

A coumarin containing hemicyanine-based probe for dual channel detection of cyanide ion

Cyanide(CN−) is known as one of the most toxic inorganic anions and is harmful to the environment and human health. Its wide use and severe toxicity have led to the development of highly selective cyanide detection, and quantification is desirable. A coumarin-containing hemicyanine-based probe has been developed for highly selective colorimetric and fluorometric detection of cyanide in acetonitrile and aqueous medium. The probe was characterized by 1D NMR (1H and 13C) and HRMS Spectroscopy, and the sensing properties have been studied in colorimetric and fluorometric methods. The high selectivity of the probe for CN− was established by UV–vis and fluorescence spectroscopy in the presence of other competing anions. The probe can detect cyanide as low as 0.7 μM within 90 s. A remarkable change in spectroscopic properties has been observed upon adding cyanide ion. The weakly luminescent probe emitted strongly upon the reaction with CN− which is due to the inhibition of internal charge transfer (ICT) from coumarin to indolinium moiety. Furthermore, the colour change from yellow to colourless and the appearance of a blue luminescence, which can be observed by the naked eye, provides a simple real-time method for cyanide detection, also supported by 1H NMR titration and theoretical study. Furthermore, a paper strip kit loaded with probe has been constructed for a real-time application of CN detection without any interference from other competitive analytes.

Aptamer-controlled gold nanozyme sensor for fluorescent and colorimetric dual-channel detection of methamphetamine

Methamphetamine (METH) is the second abused drug which affects abusers’ health and induces social crimes, developing novel methods with high sensitivity and selectivity for METH detecting is still challenging. In this paper, a colorimetric and fluorescent dual-channel sensor for METH has been constructed. We combine the enzyme-mimic catalytic activity of gold nanoparticles (GNPs) with high target specificity of METH aptamer to create a nanosensor (Apt-GNP), in the presence of METH, the absorption of 3,3′,5,5′-tetramethylbenzidine (OxTMB) at 650 nm enhanced with METH concentration increasing, while the absorption characteristic peak of GNPs at 530 nm remained almost unchanged. The ratio of A650nm/A530nm and METH concentration had a good linear relationship when METH concentration was in the range of 5–50 μM, and the corresponding linear equation is A650nm/A530nm = 0.00727CMETH (μM) + 0.783 with R2 = 0.997 and LOD = 0.40 μM (LOD = 3σ/s, n = 11). Interestingly, the fluorescence emission of Rhodamine B (RB) overlaps with the absorption spectrum of OxTMB which represents the content of METH and the fluorescence signal of RB can be quenched through internal filtering effect (IEF). Hence, when RB was doped to the detection system, the decay of RB fluorescence can reflect the concentrations change of METH. Accordingly, the linear equation is F/FR = –0.00751CMETH (μM) + 0.895 with R2 = 0.993 and LOD = 0.40 μM, where F was the fluorescence of the analytical solution at 580 nm with METH and FR was fluorescence of RB control solution. The dual-channel sensor can measure METH in serum and artificial urine successfully which is potential to be applied in drug-using crime sites and provide direct evidence to law enforcement officials.

Sulfur/nitrogen co-doped carbon-based copper nanozyme with high peroxidase-like activity for dual-channel paper-based detection of melatonin

Multiple heteroatom doping is one of the effective strategies to tune the structure of carbon supports and enhance the catalytic performance of nanozymes. Herein, the carbon-based copper nanozyme with sulfur/nitrogen co-doping (S/Cu-NC) was synthesized by one-step pyrolysis of Cu-doped ZIF-8 with thiourea encapsulated (Th/Cu@ZIF-8). Benefiting from the synergy of binary heteroatoms and 3D porous structure, the obtained S/Cu-NC had more active sites and exhibited outstanding peroxidase-like activity. Subsequently, a dual-channel (colorimetric/fluorescent) detection method for melatonin was developed by selecting 3,3,5,5-tetramethylbenzidine (TMB) and thiamine as the substrates of S/Cu-NC, which was based on the free radical scavenging effect of melatonin. Furthermore, a simple and portable paper-based device was designed to realize the rapid detection of melatonin through color changes under ultraviolet light and daylight, and the limit of detection were 0.87 μM and 1.42 μM, respectively. This paper-based device was successfully applied to detect melatonin in two kinds of drug samples with satisfactory results.

Architectural regulation of complexes for the design of metallo-supramolecular assemblies for visualizing dual-channel detection of CO

Metallo-supramolecular assembly (MSA) offers a prospective modulation tool for fabricating high-quality gas sensing platforms. However, visualizing multi-channel sensing of gaseous CO with high selectivity and sensitivity remains a formidable challenge. Here, we present a new strategy for the construction of MSA systems based on Pd(II) coordination bonds and host-guest interactions. The regulation of Pd(II) complexes is introduced into the design of MSA, and the photophysical properties, gelation behaviors, oscillatory mechanical characteristics, self-healing capabilities, and multi-stimuli responsiveness of the MSA systems can be easily modulated. The resulting two MSA systems, Pd/2BCN@6 and 2 Pd/2BCN@6, show great differences in sensing CO at solution-state (fluorescence change) and gel-state (gel-to-sol transition). Pd/2BCN@6-based gel provides a fast visual sensing (< 15 min) and efficient removal (∼15 %) of gaseous CO. 2 Pd/2BCN@6-based fluorescent system exhibits fast response (< 44 s), high selectivity and lower DLs: 7 ppm and 90.5 nM for gaseous CO and CORM-3, respectively.

Face-to-face Assembly Strategy of Au Nanocubes: Induced Generation of Broad Hotspot Regions for SERS-Fluorescence Dual-Signal Detection of Intracellular miRNAs.

While designing anisotropic noble metal nanoparticles (NPs) can enhance the signal intensity of Raman dyes, more sensitive surface-enhanced Raman scattering (SERS) probes can be designed by oriented self-assembly of noble metal nanomaterials into dimers or higher-order nanoclusters. In this study, we engineered a self-assembly strategy in living cells for real-time fluorescence and SERS dual-channel detection of intracellular microRNAs (miRNAs), using Mg2+-dependent 8-17E DNAzyme sequences as the driving motors, gold nanocubes (AuNCs) as the driver components, and three-branched double-stranded DNA as the linking tool. The assembly selects adenine in DNA as a reporter molecule, simplifying the labeling process of Raman reporter molecules and reducing the synthesis process. In addition, adenine is stably distributed between the faces of AuNCs and the wide hotspot region gives good reproducibility of the adenine SERS signal. In this strategy, the SERS channel was consistently stable and more sensitive compared to the fluorescence channel. Among them, the detection limit of the SERS channel was 2.1 pM and the coefficient of variation was 1.26% in the in vitro liquid phase and 1.49% in MCF-7 cells. The strategy successfully achieved accurate tracking and quantification of miRNA-21 in cancer cells, showing good reproducibility in complex samples as well as cells. The reported strategy provides ideas for exploring intracellular specific triggering of nanoparticles for precise control of self-assembly.

Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses

Hepatitis B virus (HBV) infection remains a major public health problem and, in associated co-infection with hepatitis delta virus (HDV), causes the most severe viral hepatitis and accelerated liver disease progression. As a defective satellite RNA virus, HDV can only propagate in the presence of HBV infection, which makes HBV DNA and HDV RNA the standard biomarkers for monitoring the virological response upon antiviral therapy, in co-infected patients. Although assays have been described to quantify these viral nucleic acids in circulation independently, a method for monitoring both viruses simultaneously is not available, thus hampering characterization of their complex dynamic interactions. Here, we describe the development of a dual fluorescence channel detection system for pan-genotypic, simultaneous quantification of HBV DNA and HDV RNA through a one-step quantitative PCR. The sensitivity for both HBV and HDV is about 10 copies per microliter without significant interference between these two detection targets. This assay provides reliable detection for HBV and HDV basic research in vitro and in human liver chimeric mice. Preclinical validation of this system on serum samples from patient on or off antiviral therapy also illustrates a promising application that is rapid and cost-effective in monitoring HBV and HDV viral loads simultaneously.

A triphenylamine-thiophene-based fluorescent probe for the dual-channel detection and imaging of hypochlorite and viscosity in live cells

In this research, a new triphenylamine-thiophene-based fluorescent probe, denoted as TTD, was constructed for simultaneous detection of viscosity and hypochlorite (ClO−) with dual-response signals. The hindrance of intramolecular bond rotation within TTD in a high-viscosity environment turns on the probe’s emission at 600 nm, resulting in strong red fluorescence. Simultaneously, the oxidation of the imine moiety to the aldehyde product, induced by ClO−, leads to substantial emission enhancement at 460 nm, generating vivid blue fluorescence. As a result, TTD can be employed for the dual-color visualization of changes in viscosity and ClO− levels, demonstrating good selectivity. TTD has been applied to image the elevated level of viscosity induced by nystatin, rotenone or erastin as well as exogenously introduced ClO− in living cells. Importantly, from two independent emission channels, TTD is capable of monitoring both ClO− and viscosity fluctuations within live cells during the inflammatory response triggered by lipopolysaccharide (LPS).

A benzimidazole-appended double-armed salamo type fluorescence and colorimetric bifunctional sensor for identification of MnO4− and its applications in actual water samples

The symmetrically double-armed salamo type fluorescent sensor BMS, incorporating benzimidazole units, was designed and synthesized. Showcasing remarkable specificity and responsiveness to MnO4- within a DMSO:H2O (V/V = 9:1, pH = 7.2) Tris-HCl buffer medium, it enabled dual-channel detection of MnO4- through fluorescent and colorimetric changes. Critical experimental parameters, including detection and quantification thresholds (LOD and LOQ) along with binding affinity constants (Ka), were calculated using the Origin software. A rational interaction mechanism between BMS and MnO4- was deduced, based on fluorescence titration, Electrospray Ionization Mass Spectrometry (ESI-MS), Ultraviolet–Visible Spectroscopy (UV–Vis), Infrared Spectroscopy (IR), Stern-Volmer plots, and Density Functional Theory (DFT) computations. Additionally, the sensor BMS was applied to monitor MnO4- in real water samples. Advancing its practical utility, BMS was fabricated into test strips for the selective detecting of MnO4-.

Design of a dual-mode ratiometric fluorescent probe via MOF-on-MOF strategy for Al (III) and pH detection

BackgroundThe construction of ratiometric fluorescent MOF sensors with integrated self-calibration and dual-channel detection can efficiently overcome the deficiencies of single-signal sensing. In this regard, the rational design of structurally functionalized MOFs is paramount for enhancing their performance in ratiometric fluorescent sensors. Lately, the concept of MOF-on-MOF design has garnered notable interest as a potential strategy for regulating the structural parameters of MOFs by integrating two or more distinct MOF types. Great efforts have been dedicated to exploring new MOF-on-MOF hybrids and developing their applications in diverse fields. Even so, these materials are still in the stage of advancement in the sensing field. ResultsHerein, a Zr-based metal-organic framework anchored on a rare-earth metal-organic framework (UiO-66(OH)2@Y-TCPP) was prepared for the ratiometric fluorescence detection toward Al (III) and pH. In this probe, the UiO-66(OH)2 featured hydroxyl active sites for Al (III), leading to a significant enhancement in fluorescence intensity upon the addition of Al (III), while the signal emitted by the red-emitting Y-TCPP, serving as the reference, remained constant. UiO-66(OH)2@Y-TCPP exhibited excellent selectivity for Al (III) sensing with a wider linear range of 0.1–1000 μM, and a lower detection limit of 0.06 μM. This probe has also been utilized for the quantitative determination of Al (III) in hydrotalcite chewable tablets with satisfactory results. In addition, the probe realized ratiometric pH sensing in the range of 7–13 using UiO-66(OH)2 as an interior reference. The paper-based probe strip was developed for visual pH sensing. By installing color recognition and processing software on a smartphone, real-time and convenient pH sensing could be achieved. SignificanceThis is the first ratiometric fluorescent sensor for Al (III) and pH detection based on a MOF-on-MOF composite probe, which yields two different response modes. The detection results of Al (III) in hydrotalcite chewable tables and smartphone imaging for pH test paper demonstrate the practicability of the probe. This work opens up a new outlook on constructing a multi-functional application platform with substantial potential for employment in environmental and biological analysis tasks.

Dual high-Q Fano resonances metasurfaces excited by asymmetric dielectric rods for refractive index sensing.

The metasurface refractive index sensor has a high degree of tunability and flexibility, providing excellent performance for high precision refractive index sensing applications. The metasurface absorber with metallic structure has been hindered in further sensor applications due to the inherent Ohmic loss of the metallic material. In this study, a dual nanorod metasurface structure based on semiconductor Si was designed, introducing a symmetry-breaking structure to excite dual ultra-narrow q-BIC resonance peaks with Fano line shapes. Both peaks are located in the near-infrared region, and multipole analysis shows that this strong field enhancement effect is induced by a magnetic dipole. Experimental results demonstrate the potential of this sensor to provide dual-channel detection while achieving high sensitivity and high Q-factor. We believe that this device exhibits outstanding performance and high practicality, providing a reference for the development and application of biological and environmental sensors.

In vivo deep brain multiphoton fluorescence imaging emitting at NIR-I and NIR-II and excited at NIR-IV.

Multiphoton microscopy (MPM) enables deep brain imaging. Three optical windows: NIR-I, NIR-II, and NIR-III are widely used. Recently, NIR-IV (the 2200 nm window) has been demonstrated to be the last and longest window for deep tissue MPM. However, so far MPM covers only two optical windows labeled by single fluorescent probe, one for emission and one for excitation. Here we demonstrate in vivo deep brain MPM covering three optical windows, with emission at NIR-I, NIR-II, and excitation at NIR-IV, labeled by ICG. The innovations include: (1) characterizing both 3-photon excitation and emission properties of ICG emitting at both NIR-I and NIR-II, in water, plasma, and circulating blood; (2) a home-built multiphoton microscope with simultaneous dual channel detection, with which we demonstrate deep brain MPM 950 μm (NIR-I) and 850 μm (NIR-II) into the mouse brain in vivo, verifying that multi-optical window MPM is promising for deep brain imaging.

Multifunctional Eu3+-MOF for simultaneous quantification of malachite green and leuco-malachite green and efficient adsorption of malachite green

Multi-target detection combined with in-situ removal of contaminants is a challenging issue difficult to overcome. Herein, a dual-emissive Eu3+-metal organic framework (Eu3+-MOF) was constructed by pre-functionalization with a blue-emissive ligand and post-functionalization with red-emissive Eu3+ ions using a UiO-66 precursor. The fluorescence of the synthesized Eu3+-MOF is highly selective and sensitive toward malachite green (MG) and its metabolite leuco-malachite green (LMG), which are environmentally persistent and highly toxic to humans. The limit of detection of MG and LMG are 34.20 and 1.98 nM, respectively. Interestingly, the fluorescence of this Eu3+-MOF showed ratiometric but different responsive modes toward MG and LMG, which enabled the simultaneous quantification of MG and LMG. Furthermore, a paper-based sensor combined with the smartphone was fabricated, which facilitated not only the dual-channel detection of MG, but also its portable, visual, rapid, and intelligent determination. Furthermore, the high surface area of MOFs, together with the coordinate bonding interaction, π–π stacking, and electrostatic interaction sites, endows Eu3+-MOF with the efficient ability toward MG removal. This multifunctional Eu3+-MOF can be successfully used for trace detection, simultaneous determination of MG and LMG, as well as efficient removal of MG. Thus, it exhibits bright prospects for widespread applications in the field of food and environmental analysis.