Root Dry Matter Accumulation Research Articles

Root Characteristics and Its Correlation with Yield of Some Maize (Zea mays L.) Genotypes in Bangladesh

The growth capacity and yield characteristics of crop plants are significantly influenced by their roots. To determine the influence of root characteristics and its correlation with yield of maize genotypes, an experiment was conducted at the experimental field of Department of Crop Botany and Tea Production Technology, Sylhet Agricultural University (SAU), Bangladesh during December 2014 to May 2015. The experimental materials were ZM 0001, ZM 0002, ZM 0003, ZM 0004, ZM 0005, ZM 0006, ZM 0007 and BARI Maize 6. Three replications and a Randomized Complete Block Design (RCBD) were used to set up the experiment. Necessary cultural practices were followed as and when require during the growing period. Data were recorded on number of roots plant-1, root length, root spreading until grain filling stage (90 days after germination) and dry matter accumulation of roots at 30 days interval until harvest. The results indicated that root number of maize genotypes increased from 30 to 90 DAG at a constant rate and in the grain filling stage and it was limited within 32.33 to 42.67. Root length was also increased from 30 to 90 DAG at a constant rate and in the grain filling stage it was limited within 25.33 to 31.1 cm. Root spreading was continued to increase from germination stage to 90 DAG and it was varied from 22 to 27.73 cm. Dry matter accumulation of roots increased gradually from 30 to 60 and 90 DAG up to harvest. In this study, root shoot ratio (RSR) and root weight ratio (RWR) had significant correlation with maize yield. From the root length and root spreading data, it is suggested that moderate tillage and a plant to plant distance spacing of 25-30 cm can be maintained for better yield of maize.

Optimization of phosphorus and potassium use efficiency of rice (Oryza sativa L) under different flood depths within the Everglades Agricultural Area

Rice cultivation in South Florida, especially within crop rotation systems, presents a promising avenue for mitigating phosphorus (P) levels in drainage water by optimizing nutrient absorption across varying flooding conditions. This study aimed to assess the impacts of different flooding depths on rice growth and nutrient efficiency while exploring the interrelationships among these factors. Conducted over a two-year period, the field study involved planting a single rice variety (Diamond: 95 kg ha−1, long-grain, short-season) at four distinct flooding depths (5, 10, 15, and 20 cm) without fertilizer application. Results revealed minimal effects of flooding depths on root and shoot length or dry matter accumulation. Additionally, concentrations of total P and potassium (K) in both shoot and root tissues remained consistent across the different flooding depths. Despite the absence of P and K fertilizer inputs, no significant changes were observed in P use efficiency (PUE), K use efficiency (KUE), or water use efficiency (WUE). Cultivating rice under optimal flood depths of 5 cm within the Everglades Agricultural Area (EAA) demonstrated potential for conserving water and essential nutrients such as P and K. These findings highlight the feasibility of rice cultivation in fertile soils as a means to decrease nutrient (P and K) concentrations in drainage water, thereby promoting sustainable agricultural practices in the region.

Effect of nano-urea and irrigation regimes on growth parameters of wheat (Triticum aestivum L.)

Wheat (Triticum aestivum L.) is a crucial cereal crop globally, serving as a staple food for a vast population. Its productivity faces challenges such as nutrient imbalance and terminal heat stress exacerbated by climate change, particularly in regions like the North-Eastern Plain Zone of India. To address these issues, a study was conducted during the rabi season of 2021–2022 in Jharkhand, India, aiming to examine the impacts of irrigation regimes and nano-urea-based nitrogen management practices on wheat. Employing a split-plot design with three replications, the experiment assessed three irrigation regimes and five nitrogen management practices. Results revealed that irrigation regimes registered non-significant effects on crop growth parameters like plant height, LAI (leaf area index), root dry weight, flag leaf area and tillers/m2 , which might be due to frequent rain up to the flowering stage during the crop growth period. Higher levels of nitrogen in commercial urea significantly increased plant height, LAI, flag leaf area, recovered root dry weight and dry matter accumulation. However, nano-urea spray at 45 DAS helped crop plants positively to increase all growth parameters more compared to nano-urea spray at 60 DAS. All growth attributes with 50% RDN+2-nano-urea spray were statistically at par with 50% RDN+1 nano-urea spray up to 40 DAS, but after the flowering stage, it was statistically similar with 100% RDN. The maximum tillers/m2 was found under 100% RDN, followed by 75% RDN+ one nano-urea spray and 50% RDN+2 nano-urea spray. Thus, 3- irrigations at CRI, flowering and milk stage and 100% RDN (120 kg N/ha) were found sufficient for enhancing the growth parameters of wheat.

Enhancing spinach (Spinacia oleracea L.) resilience in pesticide-contaminated soil: Role of pesticide-tolerant Ciceribacter azotifigens and Serratia marcescens in root architecture, leaf gas exchange attributes and antioxidant response restoration

This study unveils the detoxification potential of insecticide-tolerant plant beneficial bacteria (PBB), i.e., Ciceribacter azotifigens SF1 and Serratia marcescens SRB1, in spinach treated with fipronil (FIP), profenofos (PF) and chlorantraniliprole (CLP) insecticides. Increasing insecticide doses (25–400 μg kg−1 soil) significantly curtailed germination attributes and growth of spinach cultivated at both bench-scale and in greenhouse experiments. Profenofos at 400 μg kg−1 exhibited maximum inhibitory effects and reduced germination by 55%; root and shoot length by 78% and 81%, respectively; dry matter accumulation in roots and shoots by 79% and 62%, respectively; leaf number by 87% and leaf area by 56%. Insecticide application caused morphological distortion in root tips/surfaces, increased levels of oxidative stress, and cell death in spinach. Application of insecticide-tolerant SF1 and SRB1 strains relieved insecticide pressure resulting in overall improvement in growth and physiology of spinach grown under insecticide stress. Ciceribacter azotifigens improved germination rate (10%); root biomass (53%); shoot biomass (25%); leaf area (10%); Chl-a (45%), Chl-b (36%) and carotenoid (48%) contents of spinach at 25 μg CLP kg−1 soil. PBB inoculation reinvigorated the stressed spinach and modulated the synthesis of phytochemicals, proline, malondialdehyde (MDA), superoxide anions (O2•–), and hydrogen peroxide (H2O2). Scanning electron microscopy (SEM) revealed recovery in root tip morphology and stomatal openings on abaxial leaf surfaces of PBB-inoculated spinach grown with insecticides. Ciceribacter azotifigens inoculation significantly increased intrinsic water use efficiency, transpiration rate, vapor pressure deficit, intracellular CO2 concentration, photosynthetic rate, and stomatal conductance in spinach exposed to 25 μg FIP kg−1. Also, C. azotifigens and S. marcescens modulated the antioxidant defense systems of insecticide-treated spinach. Bacterial strains were strongly colonized to root surfaces of insecticide-stressed spinach seedlings as revealed under SEM. The identification of insecticide-tolerant PBBs such as C. azotifigens and S. marcescens hold the potential for alleviating abiotic stress to spinach, thereby fostering enhanced and safe production within polluted agroecosystems.

Effects of seedling age and root pruning on root characteristics and dry matter accumulation dynamics in machine-transplanted rice

Effects of seedling age and root pruning on root characteristics and dry matter accumulation dynamics in machine-transplanted rice

Effects of Different Tillage Depths on Soil Physical Properties and the Growth and Yield of Tobacco in the Mountainous Chongqing Region of China

Tobacco (Nicotiana tabacum L.) is the most important cash crop in the mountainous Chongqing region, where mini rotary tillers are widely used for land preparation. The decline in tobacco yields has been partially attributed to deteriorating soil physical properties and the formation of plough pans as a result of the repeated use of the mini rotary tiller. The objective of this study was to evaluate the effects of different tillage depths on soil physical properties and the growth and yield of tobacco. Three tillage depths were evaluated: mini rotary tillage to 15 cm (T15) as a control, medium-deep tillage to 25 cm (T25), and deep tillage to 35 cm (T35). Total porosity, capillary porosity, and soil water content were measured for each treatment, and the root distribution, agronomical traits, and dry matter accumulation were monitored at different growing stages. Tobacco yield and output value were determined following the harvest. Compared to T15, T35 significantly increased total porosity and capillary porosity in the 10–40 cm soil layer and soil water content in the 0–40 cm soil layer, while T25 improved soil physical properties but not significantly. T35 significantly promoted dry matter accumulation and root, stem, and leaf growth compared to T15, while differences in some agronomic traits between T25 and T15 were not significant. Compared to T15, T35 and T25 increased the tobacco yield by 17.2–18.9% and 8.0–10.1%, respectively, and increased the output value by 44.4–46.4% and 29.2–32.6%, respectively. The results indicate that deep tillage improves soil physical properties, breaks plough pans, stimulates root growth, and increases tobacco yield and output value.

Response of the root morphological structure of Fokienia hodginsii seedlings to competition from neighboring plants in a heterogeneous nutrient environment.

Critical changes often occur in Fokienia hodginsii seedlings during the process of growth owing to differences in the surrounding environment. The most common differences are heterogeneous nutrient environments and competition from neighboring plants. In this study, we selected one-year-old, high-quality Fokienia hodginsii seedlings as experimental materials. Three planting patterns were established to simulate different competitive treatments, and seedlings were also exposed to three heterogeneous nutrient environments and a homogeneous nutrient environment (control) to determine their effect on the root morphology and structure of F. hodginsii seedlings. Heterogeneous nutrient environments, compared with a homogeneous environment, significantly increased the dry matter accumulation and root morphology indexes of the root system of F. hodginsii, which proliferated in nutrient-rich patches, and the P heterogeneous environment had the most significant enhancement effect, with dry matter accumulation 70.2%, 7.0%, and 27.0% higher than that in homogeneous and N and K heterogeneous environments, respectively. Homogeneous environments significantly increased the specific root length and root area of the root system; the dry matter mass and morphological structure of the root system of F. hodginsii with a heterospecific neighbor were higher than those under conspecific neighbor and single-plant treatments, and the root area of the root system under the conspecific neighbor treatment was higher than that under the heterospecific neighbor treatment, by 20% and 23%, respectively. Moreover, the root system under heterospecific neighbor treatment had high sensitivity; the heterogeneous nutrient environment increased the mean diameter of the fine roots of the seedlings of F. hodginsii and the diameter of the vascular bundle, and the effect was most significant in the P heterogeneous environment, exceeding that in the N and K heterogeneous environments. The effect was most significant in the P heterogeneous environment, which increased fine root diameter by 20.5% and 10.3%, respectively, compared with the homogeneous environment; in contrast, the fine root vascular ratio was highest in the homogeneous environment, and most of the indicators of the fine root anatomical structure in the nutrient-rich patches were of greater values than those in the nutrient-poor patches in the different heterogeneous environments; competition promoted most of the indicators of the fine root anatomical structure of F. hodginsii seedlings. According a principal component analysis (PCA), the N, Pm and K heterogeneous environments with heterospecific neighbors and the P heterogeneous environment with a conspecific neighbor had higher evaluation in the calculation of eigenvalues of the PCA. The root dry matter accumulation, root morphology, and anatomical structure of F. hodginsii seedlings in the heterogeneous nutrient environment were more developed than those in the homogeneous nutrient environment. The effect of the P heterogeneous environment was the most significant. The heterospecific neighbor treatment was more conducive to the expansion and development of root morphology of F. hodginsii seedlings than were the conspecific neighbor and single-plant treatments.

Effects of long-term different-scale rice-duck farming on the growth and yield of paddy rice.

To maintain rice production and increase revenue, rice-duck (RD) farming is a contemporary ecological cycle technology that has been widely used in Asia. However, due to the clustering activity of duck flocks, the consequences of long-term RD farming on rice growth at different scales are still unknown. Here, we studied RD farming using several different treatments (CK: conventional rice farming; RD1: 667 m2 ; RD2: 2000 m2 ; and RD3: 3333 m2 ). The results demonstrated that the maximum tillers, effective spikes, dry matter accumulation, and lodging index of rice under RD farming were significantly decreased by 17.9%, 9.8%, 14.8%, and 17.8%, respectively, which ultimately caused a significant decrease in yield of 10.6%. However, RD farming significantly increased root oxidation activity and the ear-bearing tiller rate of rice by 25.5% and 11.1%, respectively, and improved yield stability. For different scales of RD farming, the lodging resistance index of RD1 was significantly lower than that of RD2 and RD3 by 10.0% and 15.2%, respectively, whereas the root oxidation activity and dry matter accumulation of RD2 were significantly higher than those of RD1 and RD3 by 11.1%, 4.7%, 8.6%, and 5.1%, respectively. For rice yield, there was no significant difference among the different scales. This long-term experiment helped elucidate the complicated effects of RD farming at different scales on the growth and yield of rice. It is also critical to consider the economic advantages of different scales of RD farming to assess the impact of this system more thoroughly. © 2023 Society of Chemical Industry.

Subsoiling before winter wheat alleviates the kernel position effect of densely grown summer maize by delaying post-silking root-shoot senescence

Intensified kernel position effect is a common phenomenon in maize production under higher plant density, which limits the crop productivity. Subsoiling is considered as an effective agronomic practice to improve crop productivity. In order to clarify the effect of subsoiling before winter wheat on kernel position effect of densely grown summer maize and its regulatory mechanism, field experiments were conducted during 2020-2021 and 2021-2022 growing seasons by using a split-plot design. Main plot include two tillage practices: conventional tillage practice (CT) and subsoiling before winter wheat (SS); subplot consist three plant densities (D1-D3, 6.0×104, 7.5×104, and D3, 9.0×104 plants ha-1). Compared with CT, SS alleviated the kernel position effect by increasing the weight ratio of inferior to superior kernel (WR) of D2 and D3 treated plants. The higher WR of SS treated plants attribute largely to the improved filling of inferior kernel. Under the same plant density, SS significantly improved the root dry matter accumulation (DMA) and antioxidant enzyme activities (SOD and POD), and reduced malondialdehyde (MDA) concentration, especially for the plants grown under higher plant densities. These result indicated that SS delayed the root senescence, which is associated with the reduced soil bulk density. In addition, by comparison with CT, SS increased the leaf chlorophyll content from 20 days after silking to physiological maturity and post-silking leaf area duration, and decreased post-silking leaf chlorophyll reduction rate and leaf area reduction rate, reflecting the post-silking leaf senescence is alleviated. Under the same plant density, the post-silking DMA of SS was obviously higher than that of CT, which is probably related to the improved leaf area duration and photosynthetic enzyme activity (PEPC and Rubisco). The correlation analysis revealed that the main mechanism of SS in alleviating kernel position effect of densely grown summer maize is: SS delayed the post-silking root-shoot senescence by regulating soil physical properties, and further improved the post-silking DMA and filling of inferior kernel, ultimately alleviated the kernel position effect and improved grain yield. The present result will provide a new theoretical support for the promotion of summer maize yield by subsoiling before winter wheat.

Twice-Split Phosphorus Application Alleviates Low Temperature Stress by Improving Root Physiology and Phosphorus Accumulation, Translocation, and Partitioning in Wheat

In the context of global warming, low temperature (LT) disasters in major crops are also becoming more common. LT stress in the Huang-Huai-Hai Plain, the central wheat region in China, caused a massive reduction in wheat yields. A step towards ensuring wheat yield stability and food security, this study investigated the effects of optimizing phosphorus application on the root physiology, dry matter phosphorus accumulation, translocation, and partitioning of wheat under LT stress, using the representative cultivar Yannong 19 as the test material. The treatments included conventional phosphorus application (R1) and twice-split phosphorus application (R2), followed by −4 °C LT treatment and normal temperature (NT) treatment during the anther interval stage. Analysis of the root physiology (enzymatic activities and acid phosphatase, contents of malondialdehyde, soluble sugar, and soluble protein), phosphorus and dry matter accumulation, translocation, partitioning, and agronomic and yield-related components was carried during this research study. The results showed that the wheat root activity was significantly reduced and the antioxidant enzyme activities were increased to mitigate the damage of LT stress. Moreover, LT treatments damaged root function. The root activity and antioxidant properties were significantly lower than those of the NT treatment at the flowering stage. The dry matter and phosphorus accumulations were reduced by 30.6~33.6% and 15.1~21.3% at the flowering and maturity stages, resulting in final yield losses of 10.3~13.0%. In contrast, root activity increased by 16.1~27.2% in the twice-split phosphorus application treatments, and the root antioxidant characteristics were higher. As a result, dry matter and phosphorus accumulation increased after twice-split phosphorus application and their translocation to the grains was more; the final yield increased by 5.5~7.3%. Overall, the twice-split phosphorus application enhanced the physiological function of the root system and promoted the accumulation of nutrients and their transport to the grain, and alleviated the yield loss of wheat caused by LT stress.

Combining Biometric, Biochemistry, and Photosynthetic Measurements to Analyze the Performance of Two Black Pepper Cultivars Under Water Deficit

ABSTRACT Low water availability affects plant photosynthesis, growth, and yield. In this study, physiological and biometric traits were evaluated in black pepper cultivars (Kottanadan and Bragantina) growing under water deficit conditions, according to a randomized block design, with two factors [two pepper cultivars and six water deficit levels]. Nine replicates were used for each treatment. The plant survival percentage was 90% and 10% in Kottanadan (after 12 WDD) and Bragantina (after 10 WDD), respectively. Relative water content (RWC), photosynthetic pigments, and JIP-test parameters were reduced in Bragantina pepper cultivar with water deprivation, except the basal quantum yield of the process non-photochemical in PSII (Fo/Fm), which increased with water deficit. Shoot and root dry matter accumulation, leaf number, and root volume were reduced at 10 and 12 WDD in Bragantina and Kottanadan pepper cultivars, respectively. The results obtained in this study showed that the two pepper cultivars differed strongly in their responses to the water deficit. The Kottanadan pepper cultivar showed an advantage over the Bragantina considering their high photochemical performance maintained until 12 WDD, showing better stability and efficiency in utilizing excitation energy under low water availability.

Green Grams (Vigna radiata L.) Performance in Post Mined Soils When Subjected to Organic and Inorganic Fertilizers

Though mining promotes global industrialization and economy, it disrupts natural ecosystem that calls for sustainable reclamation. A field study was conducted at Base titanium limited - Kwale County to investigate the potential of mined soils to support growth and yield of green grams when subjected to organic and inorganic fertilizers. A two factor experiment was laid down in RCBD with split plot arrangement and three replications. Green gram varieties (KS20 and N26) and amendments (organic and inorganic fertilizers) were tested. Obtained data that included: soil properties, plant height, leaf development, pods and grains per plant, root nodules, biomass, and grain yield was subjected to ANOVA using the Genstat statistical package and means ranked using Fisher's protected least significant difference at (P≤0.05). Results showed that combined manure and fertilizer significantly improved plant plant height by 36% and 36.4% for KS20 and N26 varieties respectively in season one and, 23% and 23% for KS20 and N26 varieties respectively in season two compared to unfertilized soils. Similarly, leaf development was increased by 54.9% and 52.2% for KS20 and N26 varieties respectively in season one and, 46.1% and 37% for KS20 and N26 varieties respectively in season two compared to unfertilized soils. Sole manure and sole fertilizer did not have significant effect on plant height and leaf development. Combined application of manure and fertilizer did not have significant influence on green gram nodulation, shoot and root dry matter accumulation, number of seeds per pod and pods per plant. It however increased N26 variety grain yield in the second season alone. Additionally, grains harvested from the post mined soils had adequate levels of phosphorus, Iron, Zinc, magnesium, potassium, and low levels of calcium and copper. Although the findings show that this post mined soils have the capacity to support green gram growth, further research to ascertain long term effects of green gram establishment on profile development, biological nitrogen fixation, enterprise return to capital and crops response after application of all limiting nutrients is recommended.

Optimal Substrate Moisture Content for Kiwifruit (Actinidia valvata Dunn) Seedling Growth Based on Analyses of Biomass, Antioxidant Defense, and Photosynthetic Response

The fruits of kiwifruit are well known for their abundant nutritional value and health benefits, but kiwifruit vines are susceptible to environmental factors such as drought or waterlogging. Optimum substrate moisture content (SMC) can decrease cultivation costs and improve the quality of seedlings in soilless cultivation. To quantify the water requirements of kiwifruit seedlings, a greenhouse study was conducted to investigate the growth, antioxidant defense, and photosynthetic parameters of seedlings of Actinidia valvata Dunn at six levels of SMC (20%, 40%, 60%, 80%, 100%, and 120%). Results showed that shoot and root dry matter accumulation increased gradually with the increase in SMC from 20% to 100% and was lower at 120% SMC than at 100% SMC. Electrolyte leakage and malondialdehyde content were the lowest at 80% and 100% SMC. Antioxidant enzyme activities, including superoxide dismutase, peroxidase, and catalase, chlorophyll content, net photosynthetic rate, maximal quantum yield of PSII photochemistry, photosynthetic electron transfer rate, and actual quantum yield were the highest at 80% and 100% SMC, but there was no significant difference in these parameters between the two treatments (80% and 100% SMC). However, the shoot and root dry weights of seedlings at 100% SMC were 13.20% and 33.02% higher than those at 80% SMC, respectively. In summary, 100% SMC provided optimal water supply for the photosynthetic efficiency and dry matter accumulation of shoots and roots. The results are expected to be useful for the mass production of high-quality kiwifruit seedlings in greenhouse or nursery containers, with the potential to save water.

Effects of exogenous strigolactone on growth, dry matter accumulation, and yield of tissue-cultured ginger

ABSTRACT Ginger tissue culture seedlings can reduce the incidence of soil-borne diseases. However, they have several branches, small ginger bulbs, and slow seed recovery. Strigolactone can inhibit the growth of lateral buds, adjust plant types, and increase crop yield. This study used exogenous strigolactone to treat tissue-cultured ginger and assess its effects on growth, dry matter accumulation, and yield. The results showed that the number of branches and ginger bulbs per plant decreased significantly with the application of exogenous GR24 (Synthetic analog of strigolactone, rac-GR24), and higher concentrations led to a greater decrease. Plant height, stem diameter, root, rhizome, stem sheath, leaf and plant dry matter accumulation, average ginger bulb weight, and yield increased significantly, increasing initially and then decreasing with increasing concentration. Depending on the concentrations, exogenous strigolactone can inhibit the branching of tissue-cultured ginger, reduce the number of branches, increase plant height and stem diameter, optimize the aboveground plant type, increase dry matter accumulation and root-shoot ratio, decrease the number of ginger bulbs per plant, increase the average ginger ball weight, and increase yield. When the GR24 concentration was further increased, the number of branches, the plant height, stem diameter, dry matter accumulation, root shoot ratio, ginger bulbs per plant, average weight of ginger bulbs, and ginger yield decreased. Treatment with 7.5 um L−1 of exogenous GR24 could optimize the plant type of tissue-cultured ginger and increase yield.

Short-Term Effects of Cover Crops on Soil Physical, Chemical, and Biological Properties in the Southeastern Argentinean Pampas

ABSTRACT Cover crop (CC) inclusion in cropping sequences could ameliorate negative effects of continuous agriculture on soil properties. The use of CC as a sustainable management tool can reverse the soil degradation process and improve soil health in the short term. The present study evaluated soil properties following short-term cover cropping in on-farm conditions. Three strip-trial experiments were conducted. Treatments were: i) oat (Avena sativa), ii) vetch (Vicia villosa), and iii) control (without CC). Prior to CC chemical termination, shoot and root dry matter (DM) and DM nitrogen accumulation in CC shoot (Nac) were determined. After CC termination, we determined: soil bulk density (BD), penetration resistance (PR), infiltration (INF), aggregate stability, total and particulate organic carbon (POC), organic nitrogen, nitrogen mineralized in anaerobic incubation (Nan) and soil glomalin. On average across sites, grater shoot, and root DM were determined in oat than vetch, while Nac was greater in vetch. The POC and Nan increased by CC inclusion mainly in 0–5 cm soil depth. Improvements of over 50% in INF (0–20 cm) and 8% in BD (0–5 cm) were determined in CC treatments compared to control. The effects of CC on some soil parameters depended on the previous management practices (i.e. tillage system, crop sequences). In the short-term, CC inclusion improves some soil properties, depending on their sensibility to management practices.

Combined application of organic fertilizer and chemical fertilizer alleviates the kernel position effect in summer maize by promoting post-silking nitrogen uptake and dry matter accumulation

Adjusting agronomic measures to alleviate the kernel position effect in maize is important for ensuring high yields. In order to clarify whether the combined application of organic fertilizer and chemical fertilizer (CAOFCF) can alleviate the kernel position effect of summer maize, field experiments were conducted during the 2019 and 2020 growing seasons, and five treatments were assessed: CF, 100% chemical fertilizer; OFCF1, 15% organic fertilizer+85% chemical fertilizer; OFCF2, 30% organic fertilizer+70% chemical fertilizer; OFCF3, 45% organic fertilizer+55% chemical fertilizer; and OFCF4, 60% organic fertilizer+40% chemical fertilizer. Compared with the CF treatment, the OFCF1 and OFCF2 treatments significantly alleviated the kernel position effect by increasing the weight ratio of inferior kernels to superior kernels and reducing the weight gap between the superior and inferior kernels. These effects were largely due to the improved filling and starch accumulation of inferior kernels. However, there were no obvious differences in the kernel position effect among plants treated with CF, OFCF3, or OFCF4 in most cases. Leaf area indexes, post-silking photosynthetic rates, and net assimilation rates were higher in plants treated with OFCF1 or OFCF2 than in those treated with CF, reflecting an enhanced photosynthetic capacity and improved post-silking dry matter accumulation (DMA) in the plants treated with OFCF1 or OFCF2. Compared with the CF treatment, the OFCF1 and OFCF2 treatments increased post-silking N uptake by 66.3 and 75.5%, respectively, which was the major factor driving post-silking photosynthetic capacity and DMA. Moreover, the increases in root DMA and zeatin riboside content observed following the OFCF1 and OFCF2 treatments resulted in reduced root senescence, which is associated with an increased post-silking N uptake. Analyses showed that post-silking N uptake, DMA, and grain yield in summer maize were negatively correlated with the kernel position effect. In conclusion, the combined application of 15–30% organic fertilizer and 70–85% chemical fertilizer alleviated the kernel position effect in summer maize by improving post-silking N uptake and DMA. These results provide new insights into how CAOFCF can be used to improve maize productivity.

Effect of nicosulfuron on dynamic changes in the starch-sugar interconversion in sweet maize (Zea mays L.)

Starch is an important reserve of sugar, and starch-sugar conversion in plants plays an important role in the response of plants to various abiotic stresses. Nicosulfuron is a post-emergence herbicide commonly applied to maize fields. However, it is unclear how sucrose and starch in sweet corn are converted to accommodate nicosulfuron stress. Field and pot experiments were conducted to study the effects of nicosulfuron on the sugar metabolism enzymes, starch metabolism enzymes, non-enzyme substances, and expression of key enzyme genes in leaves and roots of sweet maize seedlings. Accordingly, this research compared the responses of the sister lines HK301 and HK320, which are nicosulfuron tolerant and sensitive, respectively. Under nicosulfuron stress, compared with HK301 seedlings, the accumulation of stem and root dry matter of HK320 seedlings was significantly reduced, resulting in a lower root-to-shoot ratio. Compared with HK320 seedlings, nicosulfuron stress significantly increased the sucrose, soluble sugar, and starch contents in HK301 leaves and roots. This may be related to the enhanced carbohydrate metabolism under nicosulfuron stress, including significant changes in sugar metabolism enzyme activity and the levels of SPS and SuSys expression. Further, under nicosulfuron stress, sucrose transporter genes (SUC 1, SUC 2, SWEET 13a, and SWEET 13b) in the leaves and roots of HK301 seedlings were significantly upregulated. Our results emphasize that changes in sugar distribution, metabolism, and transport can improve the adaptability of sweet maize to nicosulfuron stress.

Insights into physiological and molecular mechanisms underlying efficient utilization of boron in different boron efficient Beta vulgaris L. varieties

Boron (B) deficiency and consequent limitation of plant yield and quality, particularly of sugar beet (Beta vulgaris L.) has emerged as a maior problem,which is exacerbating due to cultivar dependent variability in B deficiency tolerance. Pertinently, the current study was designed to elucidate the physiological and molecular mechanisms of B deficiency tolerance of sugar beet varieties KWS1197 (B-efficient variety) and KWS0143 (B-inefficient variety). A hydroponic experiment was conducted employing two B levels B0.1 (0.1 μM L−1 H3BO3, deficiency) and B50 (50 μM L−1 H3BO3, adequacy). Boron deficiency greatly inhibited root elongation and dry matter accumulation; however, formation of lateral roots stimulated and average root diameter was increased. Results exhibited that by up-regulating the expression of NIP5-1, NIP6-1, and BOR2, and suppressing the expression of BOR4, cultivar KWS1197, in contrast to KWS0143, managed to transfer sufficient amount of B to the aboveground plant parts, facilitating its effective absorption and utilization. Accumulation of malondialdehyde (MDA) and reactive oxygen species (ROS) was also mellowed in KWS1197, as well as the oxidative damage to root cells via preservation of the antioxidant enzyme system. Additionally, the expression of essential enzymes for biosynthesis of phytohormone (PYR/PYL) and lignin (COMT, POX, and CCoAOMT) were found to be highly up-regulated in KWS1197. Deductively, through effective B absorption and transportation, balanced nutrient accumulation, and an activated antioxidant enzyme system, B-efficient cultivars may cope with B deficiency while retaining a superior cellular structure to enable root development.

Allelopathy of Ricinus communis and light spectrum variation decrease emergence and growth of Cyperus rotundus

Weeds negatively influence agricultural production. However, those losses depend on weed specie, its time of emergence, and period of interference on agricultural crops. Synthetic herbicides are commonly used to control these plants species; however, they may cause damage to the environment, human beings and animals health, and this problem justify the need to develop alternative bioherbicides. To evaluate the allelopathic potential of Ricinus communis (Castor bean) and light spectrum variation on the emergence and growth of Cyperus rotundus L., a trial was carried out in a protected environment with 15% of brightness reduction at the Center for Agricultural and Environmental Sciences at the Paraíba State University. Four aqueous extract concentrations of R. communis leaves were tested (0, 5, 10, and 15%) and four light spectrums variations (white, purple, blue, and red lights). Variables such as emergence, length, dry matter accumulation and growth rates of shoots and root of C. rotundus seedlings were assessed. Data were analyzed by normality test, analysis of variance, polynomial regression, and averages test. Soot and root emergence, length, and dry matter accumulation of C. rotundus seedlings were reduced due to the allelopathy caused by R. communis aqueous extract leaves (15% concentration) and under purple or red light spectrum radiation.

Interactive impact of potassium and arbuscular mycorrhizal fungi on the root morphology and nutrient uptake of sweet potato (Ipomoea batatas L.).

Sweet potato is a typical "potassium (K)-favoring" food crop and strongly dependent on arbuscular mycorrhizal fungi (AMF). Recent studies show the importance of K and AMF to morphology optimization and nutrient uptake regulation of sweet potato; meanwhile, the interaction exists between K and K use efficiency (KIUE) in sweet potato. To date, only a few studies have shown that AMF can improve plant K nutrition, and whether the benefits conferred by AMF on plant are related to K remains unclear. In this study, low-KIUE genotype "N1" and high-KIUE genotype "Xu28" were used as experimental sweet potato; Funneliformis mosseae (FM) and Claroideoglomus etunicatum (CE) were used as experimental AMF. In a pot experiment, plants "N1" and "Xu28" were inoculated with FM or CE, and applied with or without K fertilizer to uncover the effects of K application and AMF inoculation on the root morphology and nutrient absorption of sweet potato during their growing period. Results demonstrated that AMF inoculation-improved root morphology of sweet potato highly relied on K application. With K application, AMF inoculation significantly increased root tip number of "N1" in the swelling stage and optimized multiple root morphological indexes (total root length, root surface area, root volume, root diameter, root branch number, and root tip number) of "Xu28" and CE had the best optimization effect on the root morphology of "Xu28". In addition, CE inoculation significantly promoted root dry matter accumulation of "Xu28" in the swelling and harvesting stages, coordinated aerial part and root growth of "Xu28", reduced the dry matter to leaf and petiole, and was beneficial to dry matter allocation to the root under conditions of K supply. Another promising finding was that CE inoculation could limit K allocation to the aboveground and promote root K accumulation of "Xu28" under the condition with K application. The above results lead to the conclusion that K and CE displayed a synergistic effect on root development and K acquisition of high-KIUE "Xu28". This study could provide a theoretical basis for more scientific application of AMF in sweet potato cultivation and will help further clarify the outcomes of plant-K-AMF interactions.