Drug Resistance Of Enteric Bacteria Research Articles

Antimicrobial and Herbal Drug Resistance in Enteric Bacteria Isolated from Faecal Droppings of Common House Lizard/Gecko (Hemidactylus frenatus)

From 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (IFS,18) and hostels, guest houses, and dining rooms of different canteen/mess (HGM, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. Escherichia coli were the most frequently (39) isolated followed by Citrobacter freundii (33), Klebsiella pneumonia (27), Salmonella indica (12), Enterobacter gergoviae (12), and Ent. agglomerans (11). Other important bacteria isolated from gecko droppings were Listonella damsela (2), Raoultella terrigena (3), S. salamae (2), S. houtenae (3), Edwardsiella tarda (4), Edwardsiella hoshinae (1), and Klebsiella oxytoca (2). Of the 223 isolates tested for antimicrobial drug sensitivity, 27 (12.1%) had multiple drug resistance (MDR). None of the salmonellae or edwardsiellae had MDR however, MDR strains were significantly more common among Escherichia spp. (P = 1.9 × 10−5) and isolates from IFS units (P = 3.58 × 10−23). The most effective herbal drug, Ageratum conyzoides extract, inhibited growth of only 27.8% of strains tested followed by ethanolic extract of Zanthoxylum rhetsa (13.9%), eucalyptus oil (5.4%), patchouli oil (5.4%), lemongrass oil (3.6%), and sandalwood oil (3.1%), and Artemisia vulgaris essential oil (3.1%).

Effect of low concentrations of dihydrostreptomycin on drug resistance in enteric bacteria.

Beagle dogs were fed a diet containing 0, 2, or 10 mug of dihydrostreptomycin (DSM) per g of feed. The 2-mug/g level was selected to represent a residue level of the antibiotic. In both treatment groups, medicated feed resulted in a shift from a predominantly streptomycin (SM)-susceptible coliform fecal population to an SM-resistant population. The proportion of resistant organisms was significant (P < 0.01) for both treatment groups. A definitive response did not occur with animals maintained on DSM-free diets. An increase in the prevalence of DSM-resistant organisms was observed after 15 days of DSM-supplemented feeding and persisted during the posttreatment phase of the study. The predominant pattern of resistance was SM-sulfamethoxypyridazine. Fifty-nine percent of SM-resistant strains transferred resistant determinants by conjugation to Escherichia coli K-12 recipients.

Transferable and non-transferable drug resistance in enteric bacteria from hospital and from general practice.

Drug resistance to 8 different antibiotics in Enterobacteriaceae isolated from different hospitals and two groups of general practitioners was studied. Escherichia coli dominated among the 632 strains investigated. Drug resistance was found in 62% of the 512 hospital strains and in 38% of the 120 strains from general practice. Multiple resistance was common especially in strains from hospital. R factors was found in 23% of the 317 drug-resistant strains from hospital and in 11% of the 46 drug-resistant strains from general practice. Resistance to streptomycin, sulphonamide and tetracycline either alone or in combinations were the most common traits transferred.

Transferable and non-transferable drug resistance in enteric bacteria isolated from urinary specimens in Northern Sweden.

Drug resistance was studied in Gram negative bacteria isolated from urine specimens. About 2/3 of the strains were resistant to one or more of the antibiotics under study (ampicillin, chloramphenicol, nalidixic acid, streptomycin, sulphonamide and tetracycline). Escherichia coli and Proteus dominated among the bacteria isolated. Multiple resistance was common, tetracycline resistance being the most common resistance trait. Out of 286 drug resistant strains 26 per cent were found to carry R‐factors that could be transferred to the E. coli K‐12 strain used as recipient. Also among the R‐factors, tetracycline resistance was the most common resistance. R‐factors carrying as much as 5 resistance traits were found. The frequency of R‐factors among patients from different wards and in different age groups were found to be quite similar.

Drug resistance of enteric bacteria. IX. Distribution of R factors in gram-negative bacteria from clinical sources.

Many isolates belonging to the Enterobacteriaceae were collected in 1965 from the inpatients at geographically scattered hospitals in Japan. Among 2,650 Shigella strains examined, 58.4% were found to be drug-resistant; 95.0% of these resistant strains were multiply resistant. Among 434 resistant strains examined, 81% carried R factors that were transferable by cell-to-cell contact. Of 160 isolates of other enteric bacteria, drug-resistant strains included 84.2% of the Escherichia coli, 93.0% of the Klebsiella, and 90.0% of the Proteus cultures. Among these resistant strains, 70.3% of the E. coli, 66.7% of the Klebsiella, and 52.0% of the Proteus were multiply resistant. Of these resistant strains, 84.0% of the E. coli, 88.0% of the Klebsiella, and 50.0% of the Proteus strains carried R factors. These results indicate that R factors are widespread among gram-negative bacteria of clinical significance.

Drug Resistance of Enteric Bacteria

Drug Resistance of Enteric Bacteria

Drug Resistance of Enteric Bacteria

Drug Resistance of Enteric Bacteria

Drug Resistance of Enteric Bacteria

Drug Resistance of Enteric Bacteria

Drug resistance of enteric bacteria. VII. Recombination of R factors with tetracycline-sensitive mutants.

Hashimoto, Hajime (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. VII. Recombination of R factors with tetracycline-sensitive mutants. J. Bacteriol. 92:1351-1356. 1966.-The transmissible drug-resistance factor R is able to confer resistance to tetracycline (TC), chloramphenicol (CM), streptomycin (SM), and sulfonamide (SA) on a host bacterium when infected by cell-to-cell contact. Tetracycline-sensitive mutants were isolated from either CM- or SM-sensitive mutants of an R factor. Among 30 mutants isolated, 10 were point mutants which could recombine with each other, forming recombinant R factors able to grow on plates containing 50 mug/ml of TC. The recombination frequency of TC-resistant recombinants was 10(-2) to 10(-3) in bacterial cells carrying two types of TC-sensitive R factors by superinfection with both factors. Segregational patterns of the various markers on the R factor, i.e., chl, str, sul, and m, the locus determining R mating, and their linkage order, were investigated among TC-resistant recombinants of the R factor. When TC was used as the selective drug, the tet locus mapped on the R factor as an end marker. In view of the fact that these results are inconsistent with the linkage order of various markers reported previously, a circular genetic structure for the R factor which includes five tet-s and three chl-s loci is presented.

Infectious drug resistance in enteric bacteria.

IN 1959, in Japan, Ochiai and Akiba and their collaborators first discovered that resistance to several antimicrobial agents could be transferred among enteric bacteria in vitro. This phenomenon, which has become known as infectious drug resistance, is one of special interest to the microbial geneticist, but it is also of great potential importance in clinical medicine. This paper emphasizes the medical aspects of this subject and discusses some of the genetic data that are relevant to its medical importance. A multiple-drug-resistant shigella was first isolated in Japan by Kitamoto et al. (1956) from a patient with dysentery who had just . . .

Drug resistance of enteric bacteria. VI. Introduction of bacteriophage P1CM into Salmonella typhi and formation of PldCM and F-CM elements.

Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. VI. Introduction of bacteriophage P1CM into Salmonella typhi and formation of P1dCM and F-CM elements. J. Bacteriol. 91:1787-1794. 1966.-Bacteriophage P1CM was introduced into Salmonella typhi by means of both phage infection and conjugation with Escherichia coli F(+) lysogenic for the phage. Upon incubation with a P1CM phage lysate, S. typhi and S. abony yield CM(r) cells which are lysogenic for P1CM, but S. typhimurium LT2 does not. The P1CM phage is adsorbed slightly to S. typhi, but no infectious centers are formed when the phage is plated on this strain. Tests on P1CM-adsorbing capacity of the S. typhi P1CM(+) strain and on plaque formation and transduction ability of the recovered phage from this strain indicated that the cell and the phage population did not have any special advantage over the original cell and phage population. Conjugation of S. typhi with E. coli F(+) carrying P1CM(+) gave three types of S. typhi CM(r) clones: those which carry the whole P1CM phage, those with the P1dCM element, and those with nontransferable CM(r). The second type has the F factor and is sensitive to f phages in spite of its typical behavior, serologically and biochemically, as S. typhi. It can donate the P1dCM and F(+) characters to E. coli F(-) or F(-)/P1 strains. As a consequence of conjugation with the E. coli F(+) strain, the CM(r) character of the third type of S. typhi, the nontransferable CM(r) element, acquired conjugational transferability, owing to the formation of the element, F-CM. This element can be transferred to an E. coli F(-) strain at a very high frequency (ca. 10(0)). Both the F and CM(r) determinants are jointly transduced with P1 phage and are jointly eliminated by acridine dye treatment.

Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon.

Kameda, Mitsuo (Gunma University, Maebashi, Japan), Kenji Harada, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon. J. Bacteriol. 90:1174-1181. 1965.-In the transduction of R factors with phage epsilon(15), a lysate capable of transducing the markers for (TC) or (CM.SM.SA) resistance at high frequency was obtained. The transducing agent is a defective element called epsilon(15)dR(23) which lacks certain functions of phage epsilon(15). After lysogenization with normal epsilon(15) phage and ultraviolet (UV) induction, strains carrying the epsilon(15)dR(23) element produce lysates which have a high frequency of transduction (HFT) on group E(1)Salmonella. Lytic lysates prepared on phage epsilon(15) sensitive strain with the epsilon(15)dR(23) element have a low frequency of transduction (LFT). Lytic growth of phage epsilon(34) on an epsilon(15)dR(23) strain or UV induction of an epsilon(34) lysogenic strain containing epsilon(15)dR(23) results in LFT lysates on group E(2)Salmonella. On UV induction, group E(2)Salmonella (epsilon(15) lysogens) with the epsilon(15)dR(23) element give lysates which are HFT on group E(1)Salmonella but are LFT when tested on group E(2)Salmonella. In all instances, the production of drug-resistant transductants requires infection of the cell with only a single epsilon(15)dR(23) element. It appears that the resistance region of the R factor has replaced that portion of phage genome which is essential for vegetative replication and superinfection immunity. The epsilon(15)dR(23) element does not contain the genetic determinants of the R factor responsible for transmissibility, inhibition of F mating, and interference between two R factors.

DRUG RESISTANCE OF ENTERIC BACTERIA. 3. ACQUISITION OF TRANSFERABILITY OF NONTRANSMISSIBLE R(TC) FACTOR IN COOPERATION WITH F FACTOR AND FORMATION OF FR(TC).

Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. III. Acquisition of transferability of nontransmissible R(TC) factor in cooperation with F factor and formation of FR(TC). J. Bacteriol. 88:1257-1265. 1964.-Transmissible drug-resistance factor R, which confers resistance to tetracycline, chloramphenicol, streptomycin, and sulfonamide, was previously found to be transduced in the system of the Salmonella E group with phage epsilon. The R factor of R(+) transductants was nontransmissible by cell-to-cell contact, and it was not eliminated by treatment with acridine dye. When R(+) transductants were infected with F factor, the nontransmissible R factor acquired transferability by conjugation. The R(+) conjugants, to which only the R factor was separately transmitted by conjugation from the (F(+)R(+)) donor, were still unable to transfer their R factor by conjugation. However, the (FR)(+) conjugants, to which both F and R factor were transmitted simultaneously by conjugation, were also capable of transferring their F and R factors by conjugation. From the present study, it was concluded that the recombinant (FR) factor was formed as a result of an interaction between F and R factors present in a host bacterium, and that one of the mechanisms of acquisition of transferability is accounted for by the formation of recombinant (FR) factor. The recombinant (FR) factor was transferable by conjugation, and it conferred both the drug-resistance and F(+) characters to the recipient cells. The (FR) factor was eliminated by treatment with acridine dye and also transduced as one unit into Escherichia coli K-12 by P1kc phage.

DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate capable of transducing the character of chloramphenicol resistance (CM(r)) in extremely high frequency was obtained. The transduction of the CM(r) character with the lysate was consistently accompanied by lysogenization with the phage used for transduction. This lysate exhibits no beneficial effect with normal P1, and no effect is produced by decreasing the multiplicity of infection. A single infection with the phage allows the formation of plaques as well as CM(r) lysogenic cells at the center of the plaque. Both the transducing and plaque-forming activities of the lysate were lost by neutralization with anti-P1 phage serum, and its absorption to the host bacteria was enhanced by the addition of Ca(++). Thus, it was concluded that a derivative of P1 phage (P1 CM) was isolated which had not only the ability to transduce the CM(r) character but also the capacity to form plaques; i.e., the CM(r) gene of R factor is specifically associated with the genome of phage P1. No detectable differences were noted between P1 CM and normal P1 phage in density-gradient analyses in CsCl, in stability of lysogenization, in ability to transduce chromosomal markers, and in the mode of induction from lysogenic cells by ultraviolet irradiation. The instance of transduction of the CM(r) character described here may also be considered as an example of lysogenic conversion, in the sense that the alteration in CM(r) character is inseparable from lysogenicity.

DRUG RESISTANCE OF ENTERIC BACTERIA. II. TRANSDUCTION OF TRANSMISSIBLE DRUG-RESISTANCE (R) FACTORS WITH PHAGE EPSILON.

Harada, Kenji (Gunma University, Maebashi, Japan), Mitsuo Kameda, Mitsue Suzuki, and Susumu Mitsuhashi. Drug resistance of enteric bacteria. II. Transduction of transmissible drug-resistance (R) factors with phage epsilon. J. Bacteriol. 86:1332-1338. 1963.-Transmissible drug-resistance (R) factors, which transfer resistance to tetracycline (TC), chloramphenicol, streptomycin, and sulfonamide by cell-to-cell contact, were found to be transduced in the system of Salmonella E group with phage epsilon (epsilon(15) and epsilon(34)). The R(+) transductants of S. newington (S-84) and S. chittagong (S-224) were all found to be unable to transfer their R factors by conjugation, and their R factors were not eliminated by treatment with acridine dyes so far as tested. The R factors containing TC resistance were consistently segregated when transduced. At low multiplicities of infection, the R(+) transductants with epsilon(15) were all nonlysogenic and unable to produce normal epsilon(15) phage particles; among the R(+) transductants with epsilon(34), 34% were lysogenic and 66% were sensitive to epsilon(34).

Transmissible drug resistance in an epidemic strain of Salmonella typhimurium.

Among 309 cultures ofSalmonella typhimurium, phage-type 27, fifteen, isolated from eight patients, were found to be resistant to the three drugs, streptomycin, tetracycline and sulphathiazole. This triple resistance could be transferred by growth in mixed broth culture to a strain ofSkigella sonneiand back again to sensitive cultures ofS. typhimurium. In whole cultures the resistance was stable, but spontaneous loss could be demonstrated in a small proportion of the organisms in such cultures. No elimination of resistance was demonstrated after treatment with acriflavine. Resemblances to the multiple drug resistance in enteric bacteria reported from Japan are noted.The author is most grateful to Dr E. S. Anderson, Director of the Enteric Reference Laboratory, Colindale, N.W. 9, for phage-typing the cultures ofSalmonella typhimurium, and to Dr K. Patricia Carpenter, Director of the Dysentery Reference Laboratory, for supplying cultures ofSh. sonneiwhich were essential for the experimental work.

無菌マウスおよび自然マウス腸管内における腸内細菌の薬剤耐性伝達

The transfer of the drug resistance of enteric bacteria in the intestine of germfree and conventionalmice was studied with Shigellae, Escherichiae, and Klebsiellae.Using mostly the technique described by Freter (1956), these organisms from a 24 hour broth culturewere given to the mice by stomach tube. Of course, antibiotics water were not given throughout the courseof the experiment after challenge of drug sensitive bacteria.Results obtained are as follows;1) All of the resistance factors (Streptomycine, Chloromycetin, Tetracycline, and Sulfathiazole) wereeasily transferred, when donor and recipient multiplied together in the mouse intestine.2) Transferred resistance was further retransferred to sensitive enteric bacteria in the mouse intestine.3) From four out of six experiments, resistance-transferred bacteria showed equal level and patternof resistance in each group. In two experiments, two kinds of resistance pattern were found in the transferredbacteria, but all transferred bacteria in each group showed the equality of resistance level.4) From the experiment, the transferred resistance is exactly equal, when it is passed from onedonor to other genus-differing recipients, nevertheless, if the transmission occurs between donors from differentgenus and the same recipient, the transfer-result might differ, or otherwise, equal. Therefore, it seemsthat transferred resistance factors are rather determined by donor than recipient.5) On the conclusion, it is also believed that the generation of the resistance in the human entericbacteria is due to the transmission of resistance against drug from one previously existing enteric bacteria tothe next-comer.

腸内細菌の薬剤耐性に関する研究

腸内細菌の薬剤耐性に関する研究

腸内細菌の薬剤耐性に関する研究

腸内細菌の薬剤耐性に関する研究

On the drug-resistance of enteric bacteria. 9. Drug-resistance of Escherichia coli isolated from tuberculous patients

On the drug-resistance of enteric bacteria. 9. Drug-resistance of Escherichia coli isolated from tuberculous patients