Drought-stressed Trees Research Articles

Water, not carbon, drives drought-constraints on stem terpene defense against simulated bark beetle attack in Pinus edulis.

Drought predisposes forest trees to bark beetle-induced mortality, but the physiological mechanisms remain unclear. While drought-induced water and carbon limitations have been implicated in defensive failure and tree susceptibility, evidence demonstrating how these factors interact is scarce. We withheld water from mature, potted Pinus edulis and subsequently applied a double-stem girdle to inhibit carbohydrate transport from the crown and roots. Within this isolated segment we then elicited a defense response by inoculating trees with a bark beetle-fungal symbiont (Ophiostoma sp.). We quantified local mono- and sesquiterpenes (MST), nonstructural carbohydrates (NSC), and pressure potential of the inner bark. Both drought-stressed and watered trees had similar NSC concentrations just before inoculation and depleted NSC similarly following inoculation, yet MST induction (i.e. increased concentration and altered composition) was constrained only in drought-stressed trees. Thus, NSC consumption was largely unrelated to de novo MST synthesis. Instead, stoichiometric calculations show that induction originated largely from stored resin. Watered trees experiencing higher pressure potentials consistently induced higher MST concentrations. We demonstrate the importance of preformed resin toward an induced MST response in a semi-arid conifer where drought-constraints on defense occurred through biophysical limitations (i.e. reduced turgor hindering resin transport) rather than through substrate limitation.

Watering the trees for the forest: Drought alleviation in oaks and pines by ancestral ditches

Traditional ditches (“acequias” in Spanish) derive meltwater and infiltrate groundwater providing ecological services downstream in the semi-arid Sierra Nevada range (SE Spain). Therefore, they may act as a nature-based solution by alleviating drought stress in trees growing near ditches by enhancing growth and reducing their intrinsic water-use efficiency (iWUE). Such a mitigation role of acequias is critical given that some oak (Quercus pyrenaica) and pine (Pinus sylvestris) stands reach their xeric distribution limits in Europe. We compared tree-ring width data and wood δ13C, a proxy of iWUE, in oak and pine stands located near or far (control) from ditches with different infiltration capacity in two watersheds. We assessed how trees responded to climate data, drought stress, and vegetation greenness through correlations and resilience indices. Oak trees located near ditches grew more and responded less to precipitation, soil moisture, a drought index, and greenness than control trees. In pines, we did not find this pattern, and ditch trees grew more than control trees only during an extremely dry year (1995). Climate-growth correlations suggested a longer growing season in ditch pines. Growth of ditch oaks from the “Acequia Nueva” (AN), with high infiltration capacity, responded more to autumn soil moisture and showed the lowest δ13C. Growth was enhanced by cool-wet spring conditions in pines and also by warm-wet conditions in the prior winter in the case of oaks. Control trees showed lower resistance to drought. Control trees presented higher wood δ13C values except for old oaks from the “Acequia Grande” (AG) site which may show long-term acclimation. Traditional ditches alleviate drought stress in oak and pine stands subjected to regional xeric climate conditions.

Direct and mediated impacts of mixed forests on Norway spruce infestation by European bark beetle Ips typographus

Climate change-induced windfalls and droughts exacerbate bark beetle outbreaks, severely impacting forest ecosystems. Despite extensive research on bark beetle infestation patterns, the question regarding the optimal spatial grid (SG) size for analyzing this phenomenon remains unresolved. The protective potential of natural forest complexity against bark beetles has been underestimated. We used remotely sensed data to fit Generalized Linear Models and structural equation models to explore the direct and environment-mediated effects of mixed forest cover (MFC) on Norway spruce forest infestation in central Europe. We assessed the effectiveness of various spatial grids (from 100 m to 1 km) for studying infestation by Ips typographus. We found a strong non-linear decline in infestation with increasing MFC across different spatial scales. The relationship between infestation and temperature was positive, while elevation had a negative effect on infestation, with higher infestation rates observed below 900 m. Direct effects of environmental predictors on infestation were significant at SGs of 100 m, 300 m, 400 m, and 500 m, but insignificant at 200 m and 1000 m SGs. Slope positively influenced infestation at 300 m and 400 m SGs. MFC exhibited significant indirect effects on infestation mediated by elevation, temperature, potential evapotranspiration, slope, and heat load index. Landscape variables played a significant role in the models at high-resolution spatial grids, whereas climate variables were influential in models at lower spatial grids of infestation data. We argue that mixed forest facilitates cooling, preserves water, enriches symbiotic fungal community, alleviating tree drought stress and mitigating infestation risk. Broad-leave trees’ non-host volatiles presumably disrupt olfactory signals, impeding beetles’ ability to locate host trees effectively. Our results underscore the potential of increasing mixed forest cover as a self-sustainable silvicultural measure for forest protection against I. typographus and for mitigating the effects of climate change.

Estimation of Evaporation and Drought Stress of Pistachio Plant Using UAV Multispectral Images and a Surface Energy Balance Approach

Water scarcity is a critical abiotic stress factor for plants in arid and semi-arid regions, impacting crop development and production yield and quality. Monitoring water stress at finer scales (e.g., farm and plant), requires multispectral imagery with thermal capabilities at centimeter resolution. This study investigates drought stress in pistachio trees in a farm located in Yazd province, Iran, by using Unmanned Aerial Vehicle (UAV) images to quantify evapotranspiration and assess drought stress in individual trees. Images were captured on 10 July 2022, using a Matrix 300 UAV with a MicaSense Altum multispectral sensor. By employing the Surface Energy Balance Algorithm for Land (SEBAL), actual field evapotranspiration was accurately calculated (10 cm spatial resolution). Maps of the optimum crop coefficient (Kc) were developed from the Normalized Difference Vegetation Index (NDVI) based on standard evapotranspiration using the Food and Agriculture Organization (FAO) 56 methodology. The comparison between actual and standard evapotranspiration allowed us to identify drought-stressed trees. Results showed an average and maximum daily evaporation of 4.3 and 8.0 mm/day, respectively, in pistachio trees. The real crop coefficient (Kc) for pistachio was 0.66, contrasting with the FAO 56 standard of 1.17 due to the stress factor (Ks). A significant correlation was found between Kc and NDVI (R2 = 0.67, p < 0.01). The regression model produced a crop coefficient map, valuable to support precise irrigation management and drought prevention, considering the heterogeneity at the farm scale.

Inoculation with arbuscular mycorrhiza did not affect growth, root traits or gas exchange of grafted almond saplings when exposed to drought stress

Very little is known about potential benefits of using AM inoculation as a tool to alleviate drought stress of trees grown under nursery conditions. There is some evidence that benefits of mycorrhiza on gas exchange parameters and growth of trees are proportionally greater when trees are subjected to drought stress. We hypothesized that inoculation with mycorrhiza would increase growth, nutrition, stem water potential and gas exchange of containerized almond (Prunus dulcis (Mill.) D.A. Webb) when young trees are grown under low water availability.In the spring of 2015 and 2016, forty containerized almond trees (Nonpareil scion grafted on Hansen 536, a peach/almond hybrid rootstock) were either inoculated or not with a commercially available arbuscular mycorrhizal inoculum and kept well-watered for 60 days in 2015 and 54 days in 2016, after which half of the plants were exposed to gradually reduced water availability over 38 days in 2015 and 61 days in 2016. Measurements included growth metrics, stem water potential, stomatal density, leaf NPK concentration, light saturated photosynthesis, and parameters calculated from CO2 response curves (Vcmax, J, TPU, Rd, gm, and stomatal limitation).The low-water treatment decreased stem water potential and reduced trunk diameter growth, light saturated photosynthesis (A), stomatal conductance (gs), Vcmax, J, TPU, gm, specific root length, leaf N and K concentration and total leaf N, P, and K content. Inoculation decreased root mass, increased leaf mass fraction, and decreased root mass fraction in 2015, but not in 2016. Neither stem water potential nor gas exchange were significantly altered by inoculation. Inoculation decreased leaf stomatal density, and slightly elevated leaf N and P concentrations in the low water treatments, although not consistently across years. Contrary to expectations we did not find substantially greater benefits from mycorrhizal inoculation in young drought stressed almond trees and conclude that the potential for mycorrhizal inoculant to alleviate drought stress in containerized trees is minimal.

Rapid Warming Exacerbates Winter Drought Stress in Trees at High-Altitude Areas in Northeast China

Against the background of global warming, trees in high-latitude and high-altitude areas are more sensitive to rapid warming. Revealing the response patterns of trees at different altitudes to rapid warming in typical alpine mountain environments can help to predict the future distribution of forests in the region and the associated changes in the timberline. This study used tree-ring width data (band sampling) from Dahurian larch (Larix gmelinii Rupr.) along an altitudinal gradient (970–1409 m) on Oakley Mountain to establish 10 chronologies and to analyze the growth–climate response of larch to warming and altitudinal changes along a gradient. The results showed that before rapid warming, larch growth was strongly influenced mainly by precipitation in July–August, while after warming, the growth was controlled mainly by precipitation (snowfall) in winter (October–April) and showed a significant positive correlation with the SPEI in winter and a negative correlation with temperature in February–April (early spring). This indicates that the rapid warming event led to a drastic change in the water heat balance during the pre-growth period of the trees in the study area, which shifted the tree growth from being restricted by drought in the growing season before warming to being restricted by winter drought (i.e., the lagged effect of snowfall in the pre-growing season) after warming and that the trees at low altitudes suffered from more severe winter drought. In the future, as the global climate warms further, the growth of mountain larch at low altitudes (below 1200 m) will continue to decrease, and the coupling of winter snowfall and seasonal (February–April) warming will increase the upper altitude limit at which winter drought occurs for larch in the study area; additionally, larch at higher altitudes will be able to cope with this ecological process better.

Drought increases Norway spruce susceptibility to the Eurasian spruce bark beetle and its associated fungi.

Drought affects the complex interactions between Norway spruce, the bark beetle Ips typographus and associated microorganisms. We investigated the interplay of tree water status, defense and carbohydrate reserves with the incidence of bark beetle attack and infection of associated fungi in mature spruce trees. We installed roofs to induce a 2-yr moderate drought in a managed spruce stand to examine a maximum of 10 roof and 10 control trees for resin flow (RF), predawn twig water potentials, terpene, phenolic and carbohydrate bark concentrations, and bark beetle borings in field bioassays before and after inoculation with Endoconidiophora polonica and Grosmannia penicillata. Drought-stressed trees showed more attacks and significantly longer fungal lesions than controls, but maintained terpene resin defenses at predrought levels. Reduced RF and lower mono- and diterpene, but not phenolic concentrations were linked with increased host selection. Bark beetle attack and fungi stimulated chemical defenses, yet G. penicillata reduced phenolic and carbohydrate contents. Chemical defenses did not decrease under mild, prolonged drought in our simulated small-scale biotic infestations. However, during natural mass attacks, reductions in carbon fixation under drought, in combination with fungal consumption of carbohydrates, may deplete tree defenses and facilitate colonization by I. typographus.

Fungi associated with the ambrosia beetle Xyleborus perforans (Coleoptera: Curculionidae: Scolytinae) on drought-stressed Pinus in New South Wales, Australia

Ambrosia beetles have co-evolved symbiotic relationships with an array of fungal partners. Mutualistic fungal partners are often highly successful in vertical transmission between beetle generations. These persisting relationships can alter beetle behaviour, resulting in the opportunity to occupy new ecological niches and to spread geographically. In Australia, ambrosia beetles are not currently considered a significant pest in commercial Pinus plantations, where the bark beetle Ips grandicollis is known as the primary invader of stressed trees. However, in 2019, ambrosia beetles Xyleborus perforans and X. bispinatus, co-occurring with I. grandicollis, were found to have colonised a large proportion of drought-stressed trees in commercial Pinus plantations in north-east New South Wales. In this study, X. perforans (the most prevalent of two Xyleborus spp.) was collected from infested dead and dying trees in two NSW Pinus plantations. Fungal isolates of suspected Pinus pathogens were recovered from beetle mycangia and exoskeletons as well as ambrosia beetle galleries. Morphological examination and multilocus sequence analysis identified five fungi associated with X. perforans: Fusarium parceramosum, Fusarium aff. solani, Ophiostoma ips, Raffaelea deltoideospora and Sporothrix pseudoabietina. For Australia, this is the first report of F. parceramosum, as well as the first records of O. ips, R. deltoideospora and S. pseudoabietina being vectored by Xyleborus. Pathogenicity tests were performed on seedlings of three Pinus spp., with O. ips producing significantly longer lesions than the other fungi. This study demonstrates the potential for seemingly harmless ambrosia beetles to vector plant pathogens in Australian forests, providing a mode of disease transmission that should be considered in plantation management and forest biosecurity.

Mapping Tree Water Deficit with UAV Thermal Imaging and Meteorological Data

The mapping of forest stands and individual trees affected by drought stress is a crucial step in targeted forest management, aimed at fostering resilient and diverse forests. Unoccupied aerial vehicle (UAV)-based thermal sensing is a promising method for obtaining high-resolution thermal data. However, the reliability of typical low-cost sensors adapted for UAVs is compromised due to various factors, such as internal sensor dynamics and environmental variables, including solar radiation intensity, relative humidity, object emissivity and wind. Additionally, accurately assessing drought stress in trees is a complex task that usually requires laborious and cost-intensive methods, particularly in field settings. In this study, we investigated the feasibility of using the thermal band of the Micasense Altum multispectral sensor, while also assessing the potential for modelling tree water deficit (TWD) through point dendrometers and UAV-derived canopy temperature. Our indoor tests indicated that using a limited number of pixels (< 3) could result in temperature errors exceeding 1 K. However, enlarging the spot-size substantially reduced the mean difference to 0.02 K, validated against leaf temperature sensors. Interestingly, drought-treated (unwatered) leaves exhibited a higher root mean squared error (RMSE) (RMSE = 0.66 K and 0.73 K) than watered leaves (RMSE = 0.55 K and 0.53 K), likely due to lower emissivity of the dry leaves. Comparing field acquisition methods, the mean standard deviation (SD) for tree crown temperature obtained from typical gridded flights was 0.25 K with a maximum SD of 0.59 K (n = 12). In contrast, a close-range hovering method produced a mean SD of 0.09 K and a maximum SD of 0.1 K (n = 8). Modelling the TWD from meteorological and point dendrometer data for the 2021 growth season (n = 2928) yielded an R2 = 0.667 using a generalised additive model (GAM) with vapor pressure deficit (VPD), wind speed, and solar radiation as input features. A point dendrometer lag of one hour was also implemented. When predicting individual tree TWD with UAV-derived tree canopy temperature, relative humidity, and air temperature, an RMSE of 4.92 (μm) and R2 of 0.87 were achieved using a GAM. Implementing leaf-to-air pressure deficit (LVPD) as an input feature resulted in an RMSE of 6.87 (μm) and an R2 of 0.71. This novel single-shot approach demonstrates a promising method to acquire thermal data for the purpose of mapping TWD of beech trees on an individual basis. Further testing and development are imperative, and additional data from drought periods, point dendrometers, and high-resolution meteorological sources are required.

Excerpt from Siteseeing

Between February 2021 and March 2022, Ariel Gordon and Brenda Schmidt wrote a collaborative poetry manuscript, formatted like a call and response. Ariel intended to write about urban Manitoba, the city and its trees, and Brenda was to write about rural Saskatchewan and birds. Over the course of the year, the matter of place took over and the intentions branched and flew apart. They both wrote birds and trees but also moose and mushrooms, pronghorns and wild turkeys, and people making their way through it all. They wrote climate as it was manifested in drought-stressed trees and stunted crops covered in grasshoppers, in wildfires and wildfire smoke hanging over the prairies. They wrote home as they found it.

Evaluation of drought stress level in Sargent's cherry (Prunus sargentii Rehder) using photosynthesis and chlorophyll fluorescence parameters and proline content analysis.

Sargent's cherry trees (Prunus sargentiiRehder) are widely planted as an ornamental, climate change-sensing species. This study investigated changes in the soil moisture content, fresh weight, photosynthesis and chlorophyll fluorescence properties, and the chlorophyll and proline content of four-year-old P. sargentii seedlings after 30 days of drought stress. In the trees subjected to drought stress treatment, soil moisture content decreased, and the fresh weight of the aboveground part of the plant decreased. However, there was no significant difference in the root growth of the dried plants. Among the photosynthesis parameters, Pn MAX, E and gs showed a significant (p<0.001) decrease after 15 days in dry-stressed seedlings, but there was no difference between treatments in WUE until 20 days, and there was a significant (p<0.001) difference after 24 days. Chlorophyll fluorescence parameters, Fv/Fm, ΦPSII, Rfd, NPQ, and Pn MAX, also increased after 10 days in dry-stressed seedlings, but these changes did not reach statistical significance compared to the control treatment. These results may suggest that drought stress highly correlates with photosynthesis and chlorophyll fluorescence parameters. Chlorophyll content also significantly decreased in the seedlings under drought stress compared with the control treatment. The proline content decreased until the 10th day of drought stress treatment and increased after the 15th day, showing an increase of 10.9% on the 15th day and 57.1% on the 30th day, compared to the control treatment. These results suggest that photosynthesis, chlorophyll fluorescence parameters, and proline content can be used to evaluate drought stress in trees. The results of this study can contribute to the management of forests, such as the irrigation of trees when pore control ability and photosynthesis ability decrease.

Type and duration of water stress influence host selection and colonization by exotic ambrosia beetles (Coleoptera: Curculionidae).

Fungus-farming ambrosia beetles in the tribe Xyleborini tunnel into plants and trees to establish chambers for cultivating their nutritional fungal mutualists and rearing offspring. Some xyleborine ambrosia beetles preferentially infest and perform better in living but weakened trees. Flood stress predisposes horticultural tree crops to infestation, but the impact of drought stress has not been well studied. Our objectives were to compare the effects of flood stress vs. drought stress on host selection and colonization by xyleborine ambrosia beetles and to assess the duration of flooding. Container-grown Cornus florida L. trees were flood stressed using a pot-in-pot system to submerge the roots in water while drought-stressed conditions were imposed by withholding irrigation and precipitation. When experimental trees were held under field conditions for 14 days, 7.5× more ambrosia beetles landed on stems of the flood-stressed than on the drought-stressed trees. During two additional experiments over 14 and 22 days, ambrosia beetles tunneled into the flood-stressed trees but not the drought-stressed or standard irrigation trees. By simultaneously deploying trees that were flood stressed for varying lengths of time, it was found that more tunnel entrances, and xyleborine adults and offspring were recovered from trees that were flooded for 1-16 days and 7-22 days than from trees that were flooded for 14-29 days and 28-43 days. These results indicate that acute and severe drought stress does not predispose C. florida to infestation, but flood stress and the duration of flooding influence ambrosia beetle host selection and colonization. Understanding the role of host quality on ambrosia beetle preference behavior will assist with predicting the risk of infestation of these opportunistic insects in horticultural tree crops.

Water use strategy determines the effectiveness of internal water storage for trees growing in biofilters subject to repeated droughts

Impervious surfaces create large volumes of stormwater which degrades receiving waterways. Incorporating trees into biofilters can increase evapotranspiration and therefore reduce stormwater runoff. Tree species with i) high water use, ii) drought tolerance and iii) rapid and full recovery after drought have been suggested for biofilters to maximise runoff reduction while minimising drought stress. Moisture availability fluctuates greatly in biofilter substrates and trees growing in biofilters will likely experience multiple, extended drought events that increase trade-offs between these traits. Providing an internal water storage has the potential to reduce tree drought stress and increase evapotranspiration. Two urban tree species (Agonis flexuosa and Callistemon viminalis) were grown in plastic drums with biofilter profiles. Three irrigation treatments were used: well-watered, drought with an internal water storage and drought without an internal water storage. Transpiration, leaf water potential and biomass were measured to determine the effect of biofilter internal water storage and repeated drought events on tree water use, drought stress and growth. Biofilter internal water storage improved water use and reduced drought stress for A. flexuosa, whereas C. viminalis reduced leaf loss but saw no change in water use or drought stress. A. flexuosa with biofilter internal water storage was able to recover transpiration to well-watered levels after repeated droughts, while C. viminalis experienced reduced recovery ability. It is recommended all biofilters planted with trees should have internal water storage. In systems with lower moisture availability a species with more stomatal control, such as A. flexuosa, is recommended. If selecting a species with less stomatal control, such as C. viminalis, the internal water storage volume needs to be increased to avoid drought stress.

First report of Diplodia sapinea causing Diplodia tip blight on Juniperus communis subsp. communis in Finland

In August 2022, blighted shoots were observed on juniper (Juniperus communis subsp. communis) in coastal Finland. The sites were previously confirmed to contain Scots pine (Pinus sylvestris) trees with Diplodia tip blight disease, caused by Diplodia sapinea (Terhonen, 2022) (Fig. 1). Declining shoots from 32 juniper plants were collected in four different locations in the Turku archipelago. Sixty-nine isolates of D. sapinea were taken from the borderline of living and dead phloem and sapwood of surface-sterilised juniper shoots and cultured on malt yeast peptone agar (MYP). Diplodia sapinea was isolated from all the samples. The species identification was confirmed from representative isolates using morphological characters and sequencing of the ITS region of rDNA and with species-specific primers (Adamson et al., 2021). The ITS sequences of six isolates were deposited in GenBank (Accession Nos. OP847850 - OP847855). All the isolates had 99 - 100 % identity with the sequence of the type material of D. sapinea (NR152452.1). In September 2022, branches of J. communis subsp. communis were inoculated with four different isolates of D. sapinea. Bark and phloem were removed with a cork borer (3 mm diameter) and mycelial plugs (3 mm diameter) of seven-day-old D. sapinea MYP cultures were placed on the exposed sapwood surface. The mycelial plugs were wrapped with plastic film and the branches were kept outside for five weeks and then moved to a growth chamber at 20°C with a 16 h photoperiod for an additional four weeks. The juniper branches showed necrosis in the sapwood (Fig. 2a) and occasionally a blue stain (Fig. 2b) when inoculated with D. sapinea. All control inoculations remained healthy (Fig. 2c). Diplodia sapinea was reisolated only from the necrotic lesions and from the blue stain. In November 2022, the same isolates were inoculated on two-year-old Scots pine seedlings as described by Blumenstein et al. (2022) and kept in a growth chamber at 20°C with a 16 h photoperiod for 14 days. The D. sapinea isolates caused similar symptoms in Scots pine to those observed in the field (Fig. 3a). The fungus was reisolated from the inoculated parts. The control plants stayed healthy (Fig. 3b) and no D. sapinea was isolated. This is the first report of Diplodia tip blight on J. communis subsp. communis. The disease agent, D. sapinea, was previously found in Finland on Scots pine cones collected from the ground (Müller et al., 2019) and from healthy Scots pine twigs (Terhonen et al., 2021). Besides J. communis reported here, D. sapinea has been recently demonstrated to cause Diplodia tip blight in Finland on Scots pine (Terhonen, 2022). Diplodia sapinea can be endophytic and may turn pathogenic in drought-stressed trees (Blumenstein et al., 2022). Accumulating data suggest that D. sapinea is an opportunistic pathogen which also infects alternative host species Blumenstein et al. 2022 possibly related to the high load of spores from the canopy (Caballol et al., 2022). Diplodia sapinea is a new, broad-host opportunistic pathogen in Finland and the disease Diplodia tip blight can be expected to increase in alternative hosts, especially in areas that are vulnerable to abiotic changes (for example, drought). The authors wish to thank landowners and Fredrik Granberg for helping with sample collection and Linda Mutanen for her excellent laboratory work. We thank reviewer Béatrice Henricot for her valuable comments to improve this article. This study was funded by Alfred Kordelin Foundation. The authors declare that there is no conflict of interest.

Ectomycorrhizal fungi with hydrophobic mycelia and rhizomorphs dominate in young pine trees surviving experimental drought stress

Mycorrhizal fungi can help plants to cope with drought, but research on the fungal communities that are more resistant to drought or alleviate drought stress of trees is still scarce. In this study, we investigated effects of drought on soil fungal communities and explored potential fungal traits related to drought resistance under greenhouse conditions. We manipulated water availability in pine seedlings belonging to three Spanish Pinus pinaster populations from geographical areas subjected to contrasting summer drought. A set of plant ecophysiological traits were quantified and soil fungi was quantified and profiled using ergosterol and Pacific Biosciences sequencing. Abundance of ectomycorrhizal (ECM) fungi in plants subjected to drought was lower than in well-watered plants. Most ECM taxa in plants surviving drought had long exploration types and were taxa typically forming rhizomorphs and hydrophobic mycelia. By contrast, ECM taxa in well-watered plants had wider range of distinct exploration types. No differences in fungal communities were found among P. pinaster populations. No associations between ECM fungi and plant ecophysiological traits were found, but significant interactions between drought treatments and belowground plant biomass were found for the relative abundances of ECM fungi, particularly ECM with long exploration types. Plants subjected to drought may benefit by associating to ECM taxa previously shown to transport water efficiently.

Recovery after long-term summer drought: Hydraulic measurements reveal legacy effects in trunks of Picea abies but not in Fagus sylvatica.

Climate change is expected to increase the frequency and intensity of summer droughts. Sufficient drought resistance, the ability to acclimate to and/or recover after drought, is thus crucial for forest tree species. However, studies on the hydraulics of mature trees during and after drought in natura are scarce. In this study, we analysed trunk water content (electrical resistivity: ER) and further hydraulic (water potential, sap flow density, specific hydraulic conductivity, vulnerability to embolism) as well as wood anatomical traits (tree ring width, conduit diameter, conduit wall reinforcement) of drought-stressed (artificially induced summer drought via throughfall-exclusion) and unstressed Picea abies and Fagus sylvatica trees. In P. abies, ER indicated a strong reduction in trunk water content after 5 years of summer drought, corresponding to significantly lower pre-dawn leaf water potential and xylem sap flow density. Vulnerability to embolism tended to be higher in drought-stressed trees. In F. sylvatica, only small differences between drought-stressed and control trees were observed. Re-watering led to a rapid increase in water potentials and xylem sap flow of both drought-stressed trees, and to increased growth rates in the next growing season. ER analyses revealed lower trunk water content in P. abies trees growing on throughfall-exclusion plots even 1 year after re-watering, indicating a limited capacity to restore internal water reserves. Results demonstrated that P. abies is more susceptible to recurrent summer drought than F. sylvatica, and can exhibit long-lasting and pronounced legacy effects in trunk water reserves.

Interaction of drought- and pathogen-induced mortality in Norway spruce and Scots pine.

Pathogenic diseases frequently occur in drought‐stressed trees. However, their contribution to the process of drought‐induced mortality is poorly understood. We combined drought and stem inoculation treatments to study the physiological processes leading to drought‐induced mortality in Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) saplings infected with Heterobasidion annosum s.s. We analysed the saplings' water status, gas exchange, nonstructural carbohydrates (NSCs) and defence responses, and how they related to mortality. Saplings were followed for two growing seasons, including an artificially induced 3‐month dormancy period. The combined drought and pathogen treatment significantly increased spruce mortality; however, no interaction between these stressors was observed in pine, although individually each stressor caused mortality. Our results suggest that pathogen infection decreased carbon reserves in spruce, reducing the capacity of saplings to cope with drought, resulting in increased mortality rates. Defoliation, relative water content and the starch concentration of needles were predictors of mortality in both species under drought and pathogen infection. Infection and drought stress create conflicting needs for carbon to compartmentalize the pathogen and to avoid turgor loss, respectively. Heterobasidion annosum reduces the functional sapwood area and shifts NSC allocation patterns, reducing the capacity of trees to cope with drought.

First Report of Biscogniauxia mediterranea on Quercus emoryi in Southern Arizona.

In 2019, a decline of Quercus emoryi (Emory oak) was observed on the Coronado National Forest located in southeastern Arizona. Symptoms associated with oak mortality included crown die-back and large cankers revealing charcoal-like stromal growth. Trunks and root collars showed girdling and many affected trees also displayed evidence of gold-spotted oak borer activity. Initial surveys in stands identified clusters of severe infections. Samples with black perithecia and stromal tissue were collected from symptomatic hosts. Morphological characterization of the fungus was completed on fresh perithecial tissue. Stromata were pulvinate and black showing embedded perithecial bumps, with ostioles visible from the surface of the stroma. Asci were short stipitate and cylindrical with visible oil drops, 6.6 to 9.4 (mean: 8.8) × 139.8 to 179.9 μm (mean: 166.4). Ascospores were smooth ovoid, brown to dark brown, with narrowed and round ends, 6.9 to 9.1 (mean: 7.7) × 13.8 to 25.9 μm (mean: 16.5). Colonies grown on ½ strength potato dextrose agar (½ PDA) (Korhonen 1980) at 25°C for 14 days were whitish-grey when viewed from the top and darkened embedded spheres were visible from the bottom. Single-spored cultures were isolated by dissecting the hymenium and placing in distilled water. The suspension was streaked onto ½ PDA plates and incubated for 12 hours, and pure cultures were grown on ½ PDA. DNA was isolated from a mycelial scrape and extracted using a 10% Chelex solution (Safaee 2017). The internal transcribed spacer region was amplified with the ITS1/ITS4 primers (White et al. 1990) and PCR products were sequenced at Eurofins Scientific (Lewisville, KY). Isolates (BSS1, BSS2, BSS3, BSS4) were compared to other Biscogniauxia mediterranea sequences in the NCBI database (MG098274, EF026134) and had 100% sequence identity. Pathogenicity assays were conducted using 8-year-old Emory oaks in a greenhouse. The oaks were drought stressed and watered at 50% pot capacity for two months prior to inoculation. Each tree received approximately 2400 ml of water every two weeks for the remainder of the experiment. Eight trees were wounded three times with a 5mm corer. A 5mm plug of mycelium of isolate BSS2 was used to inoculate wounds. Four trees were used as controls and inoculated with ½ PDA plugs. Wounded and inoculated areas were assessed after three months. All eight trees exhibited dark necrotic tissue in the vascular cambium and lesions ranged from 11.4 - 25.8 mm in length and were 3.9 - 13.3 mm wide. The controls exhibited no fungal sign, lesions, or necrotic tissues. The pathogen B. mediterranea was recovered from 13 of the 24 total inoculations and its identity was confirmed with ITS sequencing. Mortality and die-back caused by Biscogniauxia spp. are commonly associated with drought-stressed trees, with increasingly hot and dry conditions routinely noted as inciting factors (Desprez Loustau et al.). Emory oaks are among the dominant tree species in much of the Madrean oak woodlands of the southwestern US and Mexico and provide vital ecological and cultural services. As southwestern states continue to experience hotter and drier, conditions, it is likely Emory oak will become increasingly susceptible to die-back and mortality due to this Biscogniauxia species (Southern et al.).

Great Basin bristlecone pine mortality: Causal factors and management implications

High-elevation five-needle pines are foundational species and iconic components of subalpine forests across western North America. Because they often grow at environmental extremes, high-elevation pines are vulnerable to changing climate conditions. In addition to the direct effects of recent climatic changes, these species are increasingly threatened by biotic disturbances that thrive in the warming and drying conditions now occurring at higher elevations. Among the high-elevation pines, Great Basin bristlecone pine (Pinus longaeva) is revered for its extreme longevity and has been considered an icon of stability during periods of change. Life history strategies of Great Basin bristlecone pine that contribute to its longevity include physiological traits that enhance survival in harsh and dry habitats, and defensive traits that make it less vulnerable than other high-elevation pines to tree-killing bark beetles. Recent increases in growing degree days with no associated increase in precipitation is causing temperature-amplified tree drought stress, while warming temperatures positively influence bark beetle population growth. We report on preliminary investigations into recent and unexpected Great Basin bristlecone pine mortality at two sites, including the potential roles of weather-induced stress and bark beetles. At both sites climatic water deficit (CWD), a cumulative measure of moisture stress, and mean annual temperature increased during the 2010 decade and CWD was the highest in 2020 relative to any time during the past 40 years. Although Great Basin bristlecone pine mortality has not previously been attributed to bark beetles, we observed recent (i.e., 2013 to 2020) bark beetle-attacked trees at both sites, coincident with the timing of increasing temperature and CWD. Few adult beetles were produced, however, and our results support previous research that Great Basin bristlecone pine is a population sink for bark beetles. Because bark beetles are likely not self-sustaining in Great Basin bristlecone pine, bark beetle-caused mortality of this iconic species will most likely occur when it grows mixed with or near other pine species that support bark beetle population growth. We found Ips confusus and Dendroctonus ponderosae attacking Great Basin bristlecone pine in areas where their host trees, P. monophylla and P. flexilis, were also growing. These results suggest that the presence of these infested conifers likely contributed to Great Basin bristlecone pine mortality. We highlight several factors that may be used for prioritizing future research and monitoring to facilitate development of management strategies for protecting this iconic species.

北京山区干旱胁迫下侧柏叶片水分吸收策略

PDF HTML阅读 XML下载 导出引用 引用提醒 北京山区干旱胁迫下侧柏叶片水分吸收策略 DOI: 10.5846/stxb202010072553 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金项目（41877152）；林果业生态环境功能提升协同创新中心（市级）项目（CEFF-PXM2019_014207_000099） Leaf water absorption of Platycladus orientalis under drought stress in Beijing Author: Affiliation: Fund Project: National Natural Science( 41877152)；Project of Collaborative Innovation Center (municipal level) for forest and Fruit Industry ecological Environment Function Improvement( CEFF-PXM2019_014207_000099) 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:干旱与半干旱地区，水分是限制树木生长的重要影响因子。由于降水稀缺且分配不均，叶片吸收水分是此地区树木吸收和利用小量级降水和凝结水的主要方式。北京山区处于易旱少雨的生态脆弱地带，森林植被经常遭受干旱胁迫，所以对该地区的森林系统而言，叶片直接吸收利用截留的降雨是干旱时期树木获得水分的重要途径。基于野外对比控制试验和室内盆栽模拟试验，选取北京山区的主要造林树种侧柏为研究对象，进行利用天然降雨与模拟降雨试验，研究降雨前后侧柏叶片吸水特征，探究侧柏在干旱环境下如何通过叶片吸水缓解干旱胁迫。结果表明：当侧柏长期处于干旱胁迫状态时，叶片可以利用降雨，从中获益用来缓解树木的干旱胁迫状态；叶片的吸水能力与降雨强度呈正相关关系，与土壤含水率呈负相关关系；重度干旱下侧柏植株在降雨强度为15 mm/h时叶片吸水现象最明显，叶水势变化最大为（1.18±0.17） MPa，叶片含水率变化最大为（8.47±1.00） mg/cm2；当土壤水率高于20.8%时，基本不发生叶片吸水现象。试验结果说明在干旱地区叶片吸水是树木除根系吸水外的重要水分来源方式，并且对干旱地区有效利用短缺水资源，减轻植物水分亏缺具有重要意义。 Abstract:In arid and semi-arid areas, water is an important factor that restricts the growth of trees. Due to the scarcity and uneven distribution of precipitation, the leaf absorption of water is the main way for trees in this area to absorb and use small amounts of precipitation and condensate. The mountainous area of Beijing is located in an ecologically fragile zone prone to drought and less rain. Forest vegetation often suffers from drought stress. Therefore, for the forest system in this area, the direct absorption and use of intercepted rainfall by leaves is an important way for trees to obtain water during dry periods. Based on field comparative control experiments and indoor pot plant simulation experiments, Platycladus orientalis, the main afforestation tree species in Beijing mountainous areas, was selected as the research object. Natural rainfall and simulated rainfall experiments were carried out to study the water absorption characteristics of Platycladus orientalis leaf before and after rainfall, and explore how Platycladus orientalis under arid environment alleviate drought stress by absorbing water by leaves. The results show that when Platycladus orientalis is under drought stress for a long time, the leaves can use rainfall to alleviate the drought stress of trees; the water absorption capacity of leaves is positively correlated with rainfall intensity and negatively correlated with soil moisture content; Under severe drought, Platycladus orientalis plants have the most obvious water absorption phenomenon when the rainfall intensity is 15 mm/h. The maximum change in leaf water potential is (1.18±0.17) MPa, and the maximum change in leaf water content is (8.47±1.00) mg/cm2; When the soil moisture content is higher than 20.8%, there is basically no water absorption by the blades. The test results show that leaf water absorption in arid areas is an important source of water for trees in addition to root water absorption, and it is of great significance for effective use of scarce water resources in arid areas and alleviating plant water deficit. 参考文献 相似文献 引证文献