Bacterial biofilm-associated diseases have become the leading challenge for global food security. The inherently dense biofilm matrix provides bacteria with violent resistance to the host immune response and chemical attack. Current antimicrobials with weak biofilm-targeting options and inadequate foliar adhesion severely degrade the disruptor-biofilm interactions. To tackle these issues, the host–guest supramolecular technique was employed to optimize a bactericidal stilbene-decorated ingredient (StA8) by β-cyclodextrin (β-CD). Thus, a potent supramolecular bactericidal material (StA8@β-CD), owning both enhancive biofilm eradication and excellent foliar deposition properties, was successfully fabricated. This inclusion effectively disrupts pre-formed biofilms of Xanthomonas oryzae pv. oryzae (Xoo) and definitely kills planktonic and biofilm-enclosed bacteria. After aged Xoo cells for 48 h, subsequent StA8@β-CD treatment provides excellent biofilm eradication rate of 82.8 % at 15.68 μg mL−1. Mechanism researches disclose that StA8@β-CD inhibits exopolysaccharides (EPS) production, the transcription of swimming-associated genes, bacterial quorum sensing, and cellulase/amylase activity, all pivotal for biofilm formation and bacterial virulence. Also, this self-assembled material dramatically relieves droplet splash and rebound on hydrophobic rice leaves, thereby improving efficient utilization of agrochemicals. Combining these advantages, in vivo anti-Xoo experiments reveal that StA8@β-CD has excellent control efficiency of 50.8 % at 200 μg mL−1, quite superior to the thiodiazole-copper-20 %SC formulation (34.5 %). Besides, StA8@β-CD exhibits broad-spectrum bioactivities against citrus and kiwifruit cankers with outstanding control efficacies of 73.3 % and 73.7 %, respectively. This attempt provides a new direction for the exploitation of multipurpose supramolecular bactericidal materials.
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