Drinking Water Distribution Systems Research Articles

Opportunistic Pathogens in Drinking Water Distribution Systems—A Review

In contrast to “frank” pathogens, like Salmonella entrocolitica, Shigella dysenteriae, and Vibrio cholerae, that always have a probability of disease, “opportunistic” pathogens are organisms that cause an infectious disease in a host with a weakened immune system and rarely in a healthy host. Historically, drinking water treatment has focused on control of frank pathogens, particularly those from human or animal sources (like Giardia lamblia, Cryptosporidium parvum, or Hepatitis A virus), but in recent years outbreaks from drinking water have increasingly been due to opportunistic pathogens. Characteristics of opportunistic pathogens that make them problematic for water treatment include: (1) they are normally present in aquatic environments, (2) they grow in biofilms that protect the bacteria from disinfectants, and (3) under appropriate conditions in drinking water systems (e.g., warm water, stagnation, low disinfectant levels, etc.), these bacteria can amplify to levels that can pose a public health risk. The three most common opportunistic pathogens in drinking water systems are Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa. This report focuses on these organisms to provide information on their public health risk, occurrence in drinking water systems, susceptibility to various disinfectants, and other operational practices (like flushing and cleaning of pipes and storage tanks). In addition, information is provided on a group of nine other opportunistic pathogens that are less commonly found in drinking water systems, including Aeromonas hydrophila, Klebsiella pneumoniae, Serratia marcescens, Burkholderia pseudomallei, Acinetobacter baumannii, Stenotrophomonas maltophilia, Arcobacter butzleri, and several free-living amoebae including Naegleria fowleri and species of Acanthamoeba. The public health risk for these microbes in drinking water is still unclear, but in most cases, efforts to manage Legionella, mycobacteria, and Pseudomonas risks will also be effective for these other opportunistic pathogens. The approach to managing opportunistic pathogens in drinking water supplies focuses on controlling the growth of these organisms. Many of these microbes are normal inhabitants in biofilms in water, so the attention is less on eliminating these organisms from entering the system and more on managing their occurrence and concentrations in the pipe network. With anticipated warming trends associated with climate change, the factors that drive the growth of opportunistic pathogens in drinking water systems will likely increase. It is important, therefore, to evaluate treatment barriers and management activities for control of opportunistic pathogen risks. Controls for primary treatment, particularly for turbidity management and disinfection, should be reviewed to ensure adequacy for opportunistic pathogen control. However, the major focus for the utility’s opportunistic pathogen risk reduction plan is the management of biological activity and biofilms in the distribution system. Factors that influence the growth of microbes (primarily in biofilms) in the distribution system include, temperature, disinfectant type and concentration, nutrient levels (measured as AOC or BDOC), stagnation, flushing of pipes and cleaning of storage tank sediments, and corrosion control. Pressure management and distribution system integrity are also important to the microbial quality of water but are related more to the intrusion of contaminants into the distribution system rather than directly related to microbial growth. Summarizing the identified risk from drinking water, the availability and quality of disinfection data for treatment, and guidelines or standards for control showed that adequate information is best available for management of L. pneumophila. For L. pneumophila, the risk for this organism has been clearly established from drinking water, cases have increased worldwide, and it is one of the most identified causes of drinking water outbreaks. Water management best practices (e.g., maintenance of a disinfectant residual throughout the distribution system, flushing and cleaning of sediments in pipelines and storage tanks, among others) have been shown to be effective for control of L. pneumophila in water supplies. In addition, there are well documented management guidelines available for the control of the organism in drinking water distribution systems. By comparison, management of risks for Mycobacteria from water are less clear than for L. pneumophila. Treatment of M. avium is difficult due to its resistance to disinfection, the tendency to form clumps, and attachment to surfaces in biofilms. Additionally, there are no guidelines for management of M. avium in drinking water, and one risk assessment study suggested a low risk of infection. The role of tap water in the transmission of the other opportunistic pathogens is less clear and, in many cases, actions to manage L. pneumophila (e.g., maintenance of a disinfectant residual, flushing, cleaning of storage tanks, etc.) will also be beneficial in helping to manage these organisms as well.

Characterizing the development of biofilm in polyethylene pipes in the non-chlorinated Danish drinking-water distribution system

In newly commissioned drinking-water polyethylene (PE) pipes, biofilm develops on the inner pipe surface. The microbial community composition from colonization to the establishment of mature biofilms is less known, including the effect on the distributed water quality. Biofilm development was followed through 1.5 years in PE-pipe side streams at two locations of a full-scale, non-chlorinated drinking-water distribution system (leaving a waterworks versus 5–6 km from a waterworks) along with inlet and outlet water quality. Mature biofilms were established after ∼8–9 months, dominated by Proteobacteria, Actinobacteria and Saccharibacteria (61–93% relative abundance), with a higher diversity (OTUs/Shannon Index/16S rRNA gene amplicon sequencing) in pipes in the far end of the distribution system. Comamonadaceae, and specifically Aquabacterium (>30% of reads), dominated young (∼1.5-month-old) biofilms. Young biofilms were linked to increased microbiological counts in drinking water (HPC/ATP/qPCR), while the establishment of mature biofilms led to a drop in HPC and benefited the water quality, highlighting the importance of optimizing commissioning procedures for rapidly achieving mature and stable biofilms.

Impact of operational conditions on drinking water biofilm dynamics and coliform invasion potential.

The revelation that even low concentrations of coliforms can infiltrate into mature drinking water biofilms highlights a potential public health concern. Nowadays, the measurement of coliform bacteria is used as an indicator for fecal contamination and to control the effectiveness of disinfection processes and the cleanliness and integrity of distribution systems. In Flanders (Belgium), 533 out of 18,840 measurements exceeded the established norm for the coliform indicator parameter in 2021; however, the source of microbial contamination is mostly unknown. Here, we showed that mature biofilms, are susceptible to invasion of Serratia fonticola. These findings emphasize the importance of understanding and managing biofilms in drinking water distribution systems, not only for their potential to influence water quality, but also for their role in harboring and potentially disseminating pathogens. Further research into biofilm detachment, long-term responses to operational changes, and pathogen persistence within biofilms is crucial to inform strategies for safeguarding drinking water quality.

Cu fate driven by colloidal polystyrene microplastics with pipe scale destabilization in drinking water distribution systems

Microplastics (MPs) and Cu have been detected in drinking water distribution systems (DWDSs). Investigating MP effects on Cu adsorption by pipe scales and concomitant variations of pipe scales was critical for improving the water quality, which remained unclear to date. Therefore, polystyrene microplastics (PSMPs) were adopted for the model MPs to determine their effects on Cu fate and pipe scale stabilization, containing batch adsorption, metal speciation extraction, and Cu release experiments. Findings demonstrated that complexation and electrostatic interactions were involved in Cu adsorption on pipe scales. PSMPs contributed to Cu adsorption via increasing negative charges of pipe scales and providing additional adsorption sites for Cu, which included the carrying and component effects of free and adsorbed PSMPs, respectively. The decreased iron and manganese oxides fraction (45.57 % to 29.91 %) and increased organic fraction (48.51 % to 63.58 %) of Cu in pipe scales when PSMPs were coexisting illustrated that PSMPs had a greater affinity for Cu than pipe scales and thus influenced its mobility. Additionally, the release of Cu could be facilitated by the coexisted PSMPs, with the destabilization of pipe scales. This study was the first to exhibit that Cu fate and pipe scale stabilization were impacted by MPs, providing new insight into MP hazards in DWDSs.

A metric for drinking water service reservoir performance as a sink or source of material

Abstract Service reservoirs (SRs) are a critical component of drinking water distribution systems that impact water quality. There are no performance measures to quantify or understand this impact. By applying the concept of sink or source behaviour to describe the processes of material accumulation and mobilisation within SRs, this research develops a practical metric to facilitate assessment and quantification of SR performance. It is demonstrated how a few weeks of continuous data from instrumentation deployment at both inlet and outlet of SRs is sufficient to reveal valuable insight into SR and network performance. Through real-world applications, we provide evidence that the metric was able to track SR performance showing both beneficial and detrimental impacts on water quality, as well as quantifying seasonality and the benefits of SR cleaning. Such insight is invaluable for proactive, justifiable, and targeted decisions on the location and frequency of maintenance and management interventions.

Impact of heat islands vs. city greening: Real-time monitoring and modeling of drinking water temperature in the city of Montreal in Canada

Urbanization increases the land surface temperature through surface mineralization, adversely affecting vegetation and enhancing the urban heat island (UHI) effect. Global climate change has intensified this warming effect with more frequent and intense heatwaves during hot seasons. While these transformations influence soil temperature, their consequences on drinking water temperature within the drinking water distribution system (DWDS) remains poorly understood. Literature proposes to increase pipe burial depths to mitigate drinking water heating during summer. In this study, we monitored drinking water temperatures in a DWDS in Montreal, Canada with deeply buried pipes (average 1.8 m) during the summer of 2022, focusing on two contrasting zones in terms of UHI and green coverage. Monitoring revealed a 8°C heating effect compared to the water treatment plant, attributed to low green coverage and anthropogenic heat. Conversely, the greener zone exhibited cooler drinking water temperatures, reaching a maximum cooling effect of 8°C as compared to the temperature at the exit of the water treatment plant. Utilizing a soil and water temperature model, we predicted drinking water temperatures within the DWDS with acceptable accuracy. Soil temperature modeling results aligned well with measured water temperatures, highlighting DWDS water temperature approaching its surrounding soil temperature fairly quickly. Despite heatwaves, no immediate correlation emerged between air temperature records and measured water temperatures, emphasizing soil temperature as a superior indicator. An increase in water age displayed no correlation with an increase in measured water temperature, underscoring the dominant influence of UHI and green coverage on water temperature. These findings highlight the cooling advantages of green spaces during summer, providing valuable insights for sustainable urban planning.

Copper surface acts as good surface for biofilm attachment

The drinking water distribution system is a huge network of pipes that travel miles before reaching the household. The age of the pipe and the material affect the water quality. The water monitoring and testing parameters are limited to water quality and not pipe health. The pipe material highly impacts the biofilm formation. Copper is commonly used for its anti-microbial property, but also aids for the attachment of bacteria onto the surface. This study was aimed at analyzing the formation of biofilm on copper in a drinking water. Copper coupons were immersed in a drinking water system for a period of six months and analyzed for biofilm formation. Microbiological enumeration were done by Heterotopic plate count and found an average of 35 CFU/ml in water and 50 CFU/ml on the copper coupon. The average copper content in water was 0.01 mg/ml. SEM analysis revealed the formation of bacterial growth and found that cocci shaped bacteria were predominating on the surface of the coupons. The copper coupon serves as a surface for the attachment of bacteria and aids in the formation of biofilm.

Prediction of microbiological non-compliances using a Boosted Regression Trees model: application on the drinking water distribution system of a whole country

ABSTRACT Universal access to safe drinking water is a fundamental human right and a requirement for a healthy life. Therefore, monitoring the quality of the supplied water is of utmost importance. To achieve this goal, there is a need to develop tools that support monitoring activities and improve efficiency. Forecasting models enable the prediction of pollution levels and facilitate the implementation of action plans. In this study, the Boosted Regression Trees method was employed to investigate the variables influencing water quality failures (WQFs) due to microbial contamination at the delivery point. The dataset used was obtained from localities across the country's distribution systems. The variables under consideration included physicochemical parameters such as pH, turbidity (NTU), and free chlorine (mg L−1), along with contextual parameters like the year, season, geographic location, and locality population. Indicators of microbial contamination assessed were the presence of total coliforms, Escherichia coli, and Pseudomonas aeruginosa. The most significant variables were geographic location, free chlorine content, and the population of the locality. The model achieved an AUC value of 0.77 and provided adequate predictions in the conducted tests. It enables the exploration of key factors affecting microbiological water quality, allowing for informed action to reduce associated risks.

Manganese and iron oxides on pipe surface promote dissolved aluminum accumulation in drinking water distribution systems

Aluminum-containing deposits are pervasive in drinking water distribution systems (DWDSs). However, the mechanisms driving dissolved Al transformation to pipe deposits remain elusive. This study investigated dissolved Al accumulation in DWDSs by batch experiments and long-term pipe experiments using actual finished water. PVC pipe experiments showed that dissolved Al hardly deposited on clean PVC pipe walls at alkaline pH. However, it could be substantially anchored by the pipe surface covered with Mn and Fe deposits formed from Mn(II) oxidation and Fe(III) precipitation. Batch experiments verified that the synthesized Mn and Fe oxides exhibited a strong capacity for dissolved Al uptake at pH 7.7 and 9.0 (dissolved Al was the dominant form). Biofilms on pipe walls also enhanced dissolved Al accumulation. Iron pipe experiments showed that corroded iron pipes with abundant iron corrosion products readily accumulated Al. Compared to chlorination and chloramination, non-disinfected conditions were more favorable for particulate Al deposition on iron pipe surface, probably due to Al immobilization by biofilms. In addition, continuous Al accumulation in iron pipes enhanced Fe release to pipe water. This study highlighted the important role of metal oxides in dissolved Al accumulation in DWDSs with abundant Mn and Fe solids, which provided new insights into deposit formation and control strategies.

Prokaryotic microbial ecology as an ecosurveillance tool for eukaryotic pathogen colonisation: Meiothermus and Naegleria fowleri

Naegleria fowleri has been detected in drinking water distribution systems (DWDS) in Australia, Pakistan and the United States and is the causative agent of the highly fatal disease primary amoebic meningoencephalitis. Previous small scale field studies have shown that Meiothermus may be a potential biomarker for N. fowleri. However, correlations between predictive biomarkers in small sample sizes often breakdown when applied to larger more representative datasets. This study represents one of the largest and most rigorous temporal investigations of Naegleria fowleri colonisation in an operational DWDS in the world and measured the association of Meiothermus and N. fowleri over a significantly larger space and time in the DWDS. A total of 232 samples were collected from five sites over three-years (2016–2018), which contained 29 positive N. fowleri samples. Two specific operational taxonomic units assigned to M. chliarophilus and M. hypogaeus, were significantly associated with N. fowleri presence. Furthermore, inoculation experiments demonstrated that Meiothermus was required to support N. fowleri growth in field-collected biofilms. This validates Meiothermus as prospective biological tool to aid in the identification and surveillance of N. fowleri colonisable sites.

Protocol for responding to the detection of Legionella pneumophila in drinking water distribution systems

AbstractA protocol for responding to Legionella pneumophila detections in distribution system samples is presented and justified. The protocol was developed using existing protocols for building water systems, vetted in workshops, and provided to utilities participating in a research project. The protocol provides action levels and actions that utilities can take when L. pneumophila is detected in distribution system samples. Action levels are based on the best available science and expert judgment and are similar to those in protocols for assessing building water system monitoring results. Action levels should be reassessed as additional data and knowledge on the occurrence and growth potential of L. pneumophila in distribution systems become available. All positive detections trigger assessments that focus first on conditions local to the sample location and then extend further into the distribution system. Higher concentration or more frequent detections initiate more assertive responses and communication than lower concentration and frequency detections. The protocol provides a starting point for the development of protocols for purposes such as L. pneumophila operational surveillance or within an outbreak investigation.

PFAS promotes disinfection byproduct formation through triggering particle-bound organic matter release in drinking water pipes

Loose deposit particles in drinking water distribution system commonly exist as mixtures of metal oxides, organic materials, bacteria, and extracellular secretions. In addition to their turbidity-causing effects, the hazards of such particles in drinking water are rarely recognized. In this study, we found that trace per- and polyfluoroalkyl substances (PFASs) could dramatically promote the formation of disinfection byproducts (DBPs) by triggering the release of particle-bound organic matter. Carboxylic PFASs have a greater ability to increase chloroacetic acid than sulfonic PFASs, and PFASs with longer chains have a greater ability to increase trichloromethane release than shorter-chain PFASs. Characterization by organic carbon and organic nitrogen detectors and Fourier transform ion cyclotron resonance mass spectrometry revealed that the released organic matter was mainly composed of proteins, carbohydrates, lignin, and condensed aromatic structures, which are the main precursors for the formation of DBPs, particularly highly toxic aromatic DBPs. After the release of organic matter, the particles exhibit a decrease in surface functional groups, an increase in surface roughness, and a decrease in particle size. The findings provide new insights into the risks of loose deposits and PFASs in drinking water, not only on PFASs per se but also on its effect of increasing toxic DBPs.

Influence of drinking water biofilm microbiome on water quality: Insights from a real-scale distribution system

Biofilms, constituting over 95 % of the biomass in drinking water distribution systems, form an ecosystem impacting both the aesthetic and microbiological quality of water. This study investigates the microbiome of biofilms within a real-scale drinking water distribution system in eastern Spain, utilizing amplicon-based metagenomics. Forty-one biofilm samples underwent processing and sequencing to analyze both bacterial and eukaryotic microbiomes, with an assessment of active biomass. Genus-level analysis revealed considerable heterogeneity, with Desulfovibrio, Ralstonia, Bradyrhizobium, Methylocystis, and Bacillus identified as predominant genera. Notably, bacteria associated with corrosion processes, including Desulfovibrio, Sulfuricella, Hyphomicrobium, and Methylobacterium, were prevalent. Potentially pathogenic bacteria such as Helicobacter, Pseudomonas, and Legionella were also detected. Among protozoa, Opisthokonta and Archaeplastida were the most abundant groups in biofilm samples, with potential pathogenic eukaryotes (Acanthamoeba, Naegleria, Blastocystis) identified. Interestingly, no direct correlation between microbiota composition and pipe materials was observed. The study suggests that the usual concentration of free chlorine in bulk water proved insufficient to prevent the presence of undesirable bacteria and protozoa in biofilms, which exhibited a high concentration of active biomass.

Impact of changes in biofilm composition response following chlorine and chloramine disinfection on nitrogenous disinfection byproduct formation and toxicity risk in drinking water distribution systems

In practical drinking water treatment, chlorine and chloramine disinfection exhibit different mechanisms that affect biofilm growth. This study focused on the influence of biofilm composition changes, especially extracellular polymeric substance (EPS) fractions, on the potential formation and toxicity of nitrogenous disinfection by-products (N-DBP). Significant differences in microbial diversity and community structure were observed between the chlorine and chloramine treatments. Notably, the biofilms from the chloramine-treated group had higher microbial dominance and greater accumulation of organic precursors, as evidenced by the semi-quantitative confocal laser-scanning microscopy assay of more concentrated microbial aggregates and polysaccharide proteins in the samples. Additionally, the chloramine-treated group compared with chlorine had a higher EPS matrix content, with a 13.5 % increase in protein. Furthermore, the protein distribution within the biofilm differed; in the chlorine group, proteins were concentrated in the central region, whereas in the chloramine group, proteins were primarily located at the water–biofilm interface. Notably, functional prediction analyses of protein fractions in biofilms revealed specific functional regulation patterns and increased metabolism-related abundance of proteins in the chlorine-treated group. This increase was particularly pronounced for proteins such as dehydrogenases, reductases, transcription factors, and acyl-CoA dehydrogenases. By combining the Fukui function and density functional calculations to further analyse the effect of biofilm component changes on N-DBP production under chlorine/chloramine and by assessing the toxicity risk potential of N-DBP, it was determined that chloramine disinfection is detrimental to biofilm control and the accumulation of protein precursors has a higher formation potential of N-DBPs and toxicity risk, increasing the health risk of drinking water.

The insightful water quality analysis and predictive model establishment via machine learning in dual-source drinking water distribution system

Dual-source drinking water distribution systems (DWDS) over single-source water supply systems are becoming more practical in providing water for megacities. However, the more complex water supply problems are also generated, especially at the hydraulic junction. Herein, we have sampled for a one-year and analyzed the water quality at the hydraulic junction of a dual-source DWDS. The results show that visible changes in drinking water quality, including turbidity, pH, UV254, DOC, residual chlorine, and trihalomethanes (TMHs), are observed at the sample point between 10 and 12 km to one drinking water plant. The average concentration of residual chlorine decreases from 0.74 ± 0.05 mg/L to 0.31 ± 0.11 mg/L during the water supplied from 0 to 10 km and then increases to 0.75 ± 0.05 mg/L at the end of 22 km. Whereas the THMs shows an opposite trend, the concentration reaches to a peak level at hydraulic junction area (10–12 km). According to parallel factor (PARAFAC) and high-performance size-exclusion chromatography (HPSEC) analysis, organic matters vary significantly during water distribution, and tryptophan-like substances and amino acids are closely related to the level of THMs. The hydraulic junction area is confirmed to be located at 10–12 km based on the water quality variation. Furthermore, data-driven models are established by machine learning (ML) with test R2 higher than 0.8 for THMs prediction. And the SHAP analysis explains the model results and identifies the positive (water temperature and water supply distance) and negative (residual chlorine and pH) key factors influencing the THMs formation. This study conducts a deep understanding of water quality at the hydraulic junction areas and establishes predictive models for THMs formation in dual-sources DWDS.

Applying microelectrodes to investigate aged ductile iron and copper coupon reactivity during free chlorine application

In drinking water distribution systems, including premise plumbing, dissolved oxygen (DO) and free chlorine (FC) are common oxidants and ductile iron (DI) and copper (Cu) are commonly used pipe materials. Microelectrodes as a tool have been applied in previous corrosion research and were used in this study to collect quantifiable data and understand DO and FC reactivity and pH changes at the water-metal interface. Using microelectrodes, pH, DO, and FC profiles from the bulk water to near and at the surface of aged DI (154–190 d) and Cu (2 d and 86–156 d) coupons were investigated during periods of flow and stagnation (30 min). Using the measured microelectrode profiles, oxidant fluxes and apparent surface reaction rate constants were calculated to elucidate differences between DO and FC reactivity with the coupons. Microelectrodes were successfully applied to measure pH, DO, and FC profiles from the bulk water to near aged DI and Cu coupon surfaces; Cu coupons aged quickly and exhibited less reactivity at 2 d with DO and FC than aged DI coupons did after 154–190 d; and for the aged DI coupon experiments, orthophosphate presence stabilized pH profiles where without orthophosphate pH fluctuations of greater than 2 pH units occurred from the bulk water to the DI coupon surface.

Study on Drinking Water Distribution System at Sekaran District Service Area in Lamongan Regency

Drinking Water Supply System (SPAM) organizers needs to carry out its duties and functions to fulfil the quality, quantity, and continuity for the community. From all SPAM service areas in Sekaran District, there are three villages, namely Manyar, Jugo, and Trosono Village which lack access to water. The three villages water pressure falls below the minimum pressure requirements of Minister of Public Works and Housing Regulation Number 27/RT/M/2016. Seeing that the continuity is not optimal, it is important to study the drinking water distribution network of one of the SPAM in District Sekaran service areas. Recommendations for pipeline network restoration involves conducting hydraulic simulation using EPANET 2.2 which identified the need to parallelize existing pipes and establish a new pipe with a diameter of 200 mm and a length of 1960 m that connects Ngarum Village and Jugo Village. In addition, expanded reservoir capacity is required to meet water demand because it will increase during the development year. Furthermore, based on the results of the demand pattern measurement, the pump schedule must be modified, as well as the quantity of functioning pumps to be able to meet the water supply demand when the development occurs.

Natural Organic Matter Character in Drinking Water Distribution Systems: A Review of Impacts on Water Quality and Characterization Techniques

Natural Organic Matter (NOM) in water arises from decomposed plant and animal matter and is ubiquitous in drinking water sources. The variation in NOM concentrations and characteristics, influenced by events like floods and droughts, plays a crucial role in water treatment efficiency and water quality received by the public. For example, increased NOM concentrations necessitate higher levels of coagulants and disinfectants, leads to the formation of disinfection by-products (DBPs), and plays a key role in biofilm development. When considering impacts of NOM, it is not only the presence or concentration but the makeup or proportion of varying sub-groups which can impact water quality. Formation of DBPs, corrosion and scaling, pollutant transport, aesthetic deterioration, and biofilm growth are dependent on the relative composition of NOM within the distribution system. Although the role of NOM concentration and characteristics is well studied during treatment, the impacts of residual NOM in water distribution systems have received less attention. In particular, it is clear, due to the varying roles of NOM sub-groups, that greater consideration of NOM characteristics in distribution systems is needed. This paper reviews the broad implications of NOM characteristics for water distribution systems and explores challenges and opportunities in NOM characterization within distribution systems. Furthermore, the influence of NOM characteristics in premise plumbing is examined. The review highlights the necessity for precise NOM characterization and real-time monitoring, aiming to strengthen water distribution system resilience.

Biofilm Formation in Water Distribution Systems

A biofilm is a biologically active matrix attached to the surface of cells and their extracellular products. As they are a mixture of many microorganisms, the microbiological activity of biofilms varies according to their position in the aggregate. With particular emphasis on drinking water distribution systems, this review focuses on the process of biofilm formation, associated bacteria, chlorine resistance of bacteria, and the predominant surface materials. We have compiled studies on the bacteria in drinking water distribution systems and their interactions with biofilm formation on different materials, and we also analysed the chlorine-resistant bacteria and their problems in the water networks. The materials used in the drinking water network are significantly affected by the disinfection method used to produce the biofilm that adheres to them. Some studies propose that the material is inconsequential, with the disinfection process being the most significant factor. Studies suggest that materials based on plastics (such as PVC and HDPE) tend to be more effective in controlling biofilm formation or removal than those based on metals (such as stainless steel), which have been found to be less effective in some instances. Chlorine-resistant strains are becoming more and more common in drinking water networks, resulting in the occurrence of diseases such as typhus and cholera.

From source to house: unraveling the seasonal effect of water distribution system on drinking water quality of poultry farms under Egyptian environmental condition.

Improvements in drinking water quality (DWQ) can lead, according to some estimates, to a 10% reduction of the world's disease load. The drinking water distribution system (DWDS) plays a crucial role in influencing DWQ and can contribute to the emergence of poultry-related epidemics. This study aims to monitor the variations in DWQ throughout the seasons within the DWDS of Egyptian poultry farms experiencing epidemics. The study assessed DWQ at four different points along the DWDS, including the water source (WS), water tank (WT), broiler drinker (BD), and layer drinker (LD), across 86 farms. Statistical analysis was employed to establish correlations between DWQ and the sampling points within the DWDS, as well as between water temperature (Tw.C°), ambient temperature (Ta.C°), and microbial DWQ. The survey revealed significant differences between Tw.C° and Ta.C°, with notable effect sizes (d = 0.89-1). Additionally, the results revealed significant differences in physicochemical DWQ between WS and house drinkers (HD), with medium to large effect sizes (d = 0.56-0.85). Furthermore, significant differences were identified in microbial DWQ between winter and summer, with a small to large effect size (d = 0.40-0.87). Notably, we recorded significant differences in microbial DWQ between WS and WT, with a small to medium effect size (d = 0.40-0.61), and between WT and BD, with a small to medium effect size (d = 0.48-0.53). Additionally, we found significant differences in microbial DWQ between WS and LD, with a medium effect size (d = 0.59-0.68). In conclusion, Tw.C° is influenced by seasonal variations in Ta.C°. While the physicochemical DWQ was unaffected by seasonal temperature variations, it was significantly impacted by the DWDS from WS to HD. In contrast, the microbial DWQ was strongly influenced by both seasonal temperature changes and DWDS.