Drinking Tap Water Research Articles

Qualitative and Quantitative Analyses of Microplastics in Tap Water Supply Network in Iran

Background: Microplastics (MPs) exposure can affect humans in various ways, with tap water being one of the potential sources. Microplastics can absorb other pollutants and pose risks to both humans and the environment. Objectives: The aim of this study was to investigate the presence of MPs in tap water from the drinking water distribution system of Isfahan, Iran, and to determine the exposure to MPs from drinking tap water. Methods: Samples were collected from different points in the drinking water distribution system of Isfahan, Iran. Samples were prepared for analysis through filtration and chemical digestion. The MPs were counted using a scanning electron microscope (SEM), and the composition of MPs was analyzed using a micro-Raman spectrometer. Results: The concentration of MPs in tap water was found to be 287.0 ± 65.9 MPs/l. MPs ≤ 10 µm were the predominant sizes, and fibers were the predominant shape. Polyethylene terephthalate, polyethylene, and polypropylene were the most frequently identified MPs, respectively. Conclusions: Considering that the Iranian population consumes 7 - 15 liters of water daily for drinking and cooking, it is estimated that the average intake of MPs through cooking and drinking water is 2009 - 43,051 particles per day.

Bottled or Tap Water? Factors Explaining Consumption and Measures to Promote Tap Water

The production and consumption of plastic bottled water have several negative environmental impacts worldwide. To identify the barriers and motivations for drinking tap and bottled water, we conducted a nationally representative questionnaire survey among 3411 respondents in the Czech Republic in 2022. People aged 18–34 are moderate consumers of bottled water and very frequent consumers of tap water. Bottled water consumption tends to be less frequent among people with a higher education, while tap water consumption is less frequent among people with lower incomes. The most important factors that explain the frequency of drinking bottled and tap water are taste perception, health concerns and habit. Health concerns about tap water and the unpleasant taste of tap water increase the consumption of bottled water. People with a strong habit of drinking tap water are less likely to consume bottled water. The constructs from the theory of planned behaviour were statistically significant. The results can guide decision-makers in promoting tap water to consumers. To encourage tap water drinking, we suggest measures to increase the availability of tap water in public places in conjunction with campaigns targeting the taste and health perception, as well as the habit, of drinking tap water.

Carcinogenic risk to the health of the population of a large industrial city as a result of multi-environmental and multi-route exposure to chemicals

Introduction. Despite the relatively low average annual mortality rate, the increased incidence of cancer in the population of the Omsk region, together with indicators of the quality of oncological care, indicate a tense epidemiological situation. The purpose of the study is to determine priority environments and routes of exposure to chemicals in the formation of long-term carcinogenic risks in the city of Omsk. Materials and methods. There were used results of environmental and social-hygienic monitoring of habitat objects for the period 2017–2022. To assess the cumulative carcinogenic risk, atmospheric air, drinking tap water, open water, soil, food products, and 3 routes of exposure were taken into account: inhalation, oral and cutaneous. The assessment of carcinogenic risks was carried out in accordance with Guideline R 2.1.10.3968–23, taking into account the severity coefficient of malignant neoplasms. Results. The carcinogenic risk to the health of the population of the city of Omsk as a result of multi–environmenal and multi-route exposure to chemicals was assessed as alarming (high in an the engraved script). The structure of contributions to the cumulative carcinogenic risk: drinking water – 63.3%, atmospheric air – 33.1%, food products – 3.3 %, other environments less than 0.5%. The main route of entry of chemical carcinogens is inhalation (77.4%). Alarming carcinogenic risks were formed through the inhalation route of chloroform from drinking water; chromium VI, formaldehyde, benzene, and soot from atmospheric air. Among the target organs of malignant neoplasms, the greatest risk was in the gastrointestinal tract, which was assessed as alarming. Limitations. Not all habitat objects were analyzed, which may reduce the objectivity of the conclusions. Conclusion. An assessment of the long-term cumulative carcinogenic risk made it possible to prioritize the list of chemical pollutants, environmental objects, and routes of exposure to carcinogens. For more accurate planning of sanitary and hygienic measures to reduce public health risks, improve comprehensive monitoring of pollutants in all environments, taking into account all routes of entry into the human body, it is advisable to analyze the total risks for multi-environmental and multi-route exposure with an analysis of the contribution of chemicals, media, routes to their formation.

Feasibility of increasing calcium content of drinking tap water following quality regulations to improve calcium intake at population level.

Calcium intake is below recommendations in several parts of the world. Improving calcium intake has benefits not only for bone health but also helps to prevent pregnancy hypertension disorders. Calcium concentration of tap water is usually low The aim of the present study was to determine the maximum amount of calcium that can be added to tap water while complying with drinking water Argentine regulations. Tap water samples were collected from the Province of Buenos Aires (Argentina). Physicochemical properties and saturation index were measured. Different incremental concentrations of calcium chloride were added to the experimental aliquots. Baseline water had a mean calcium concentration of 22.00 ± 2.54 mg/L, water hardness of 89.9 ± 6.4 mg/L CaCO 3, and a saturation index of -1.50 ± 0.11. After the addition of 0.4554 ± 0.0071 g of salt, water hard-ness reached 355.0 ± 7.1 mg/L CaCO 3, a calcium concentration of 140.50 ± 2.12 mg/L, and a saturation index -0.53 ± 0.02. This study shows that at laboratory level it is feasible to increase calcium concentration of drinking water by adding calcium chloride while complying with national standards. Calcium concentration of drinking tap water could be evaluated and minimum calcium concentration of tap water regulated so as to improve calcium intake in populations with low calcium intake.

Filling Discrepancies between Consumer Perception and Actual Piped Water Quality to Promote the Potable Use of the Municipal Water Supply in Indonesia

Although piped water is a safely managed water source, many people prefer drinking packaged/bottled water, which undermines environmental sustainability by creating waste plastics. Thus, to encourage drinking tap water, this study aimed to identify and fill the discrepancy between consumers’ perception of and the actual quality of the municipal water supply in Indonesia. Through questionnaire surveys in three cities, i.e., Malang, Bogor, and Semarang cities, we found that the majority of the residents, namely, 82%, 58%, and 66%, respectively, drank packaged water. Although the piped water supply from Perusahaan Daerah Air Minum (PDAM) has a good appearance, color, taste, and water quality, sporadic microbial contamination was detected due to the depletion of residual chlorine. However, packaged water was also contaminated by indicator bacteria owing to storage methods. There was a notable discrepancy between the residents’ perceptions of tap water safety and their choices of drinking water sources. The residents perceived spring water as a clean source, which influenced their selection of water sources more than the costs of water. Public drinking stations installed by PDAM helped to win consumers’ trust in PDAM water, indicating that installing more public drinking stations will encourage more people to drink piped water.

Estimating the risk of gastrointestinal illness associated with drinking tap water in Norway: a prospective cohort study

BackgroundThe delivery of safe drinking water has high public health relevance, as reflected in the Sustainable Development Goals (SDG6). Several precautionary actions have reduced the burden associated with infectious diseases in high-income countries; however, pollution in source waters, inadequate disinfection, and premise plumbing, along with an increased awareness that intrusion in the drinking water distribution system, represents risk factors for gastrointestinal illness linked to consume of drinking water. Sporadic cases of waterborne infections are expected to be underreported since a sick person is less likely to seek healthcare for a self-limiting gastrointestinal infection. Hence, knowledge on the true burden of waterborne diseases is scarce. The primary aim with the present study was to estimate the risk of gastrointestinal illness associated with drinking tap water in Norway.MethodsWe conducted a 12-month prospective cohort study where participants were recruited by telephone interview after invitation based on randomised selection. A start up e-survey were followed by 12 monthly SMS questionnaires to gather information on participants characteristics and drinking tap water (number of 0.2L glasses per day), incidence, duration and symptoms associated with gastrointestinal illness. Associations between the exposure of drinking tap water and the outcome of risk of acute gastrointestinal illness (AGI) were analysed with linear mixed effects models. Age, sex, education level and size of the drinking water supply were identified as potential confounders and included in the adjusted model.ResultsIn total, 9,946 persons participated in this cohort study, accounting for 11.5% of all invited participants. According to the data per person and month (99,446 monthly submissions), AGI was reported for 5,508 person-months (5.5 per 100 person-months). Severe AGI was reported in 819 person-months (0.8 per 100 person-months). Our study estimates that 2–4% of AGI in Norway is attributable to drinking tap water.ConclusionsThis is the largest cohort study in Norway estimating the burden of self-reported gastrointestinal infections linked to the amount of tap water drunk in Norway. The data indicate that waterborne AGI is not currently a burden in Norway, but the findings need to be used with caution. The importance of continued efforts and investments in the maintenance of drinking water supplies in Norway to address the low burden of sporadic waterborne cases and to prevent future outbreaks needs to be emphasised.

Association between drinking water sources and cognitive functioning in Chinese older adults residing in rural areas.

To explore the association between drinking water sources and cognitive functioning among older adults residing in rural China. Data were extracted from the 2008-2018 Chinese Longitudinal Healthy Longevity Survey. Drinking water sources were categorized according to whether purification measures were employed. The Chinese version of the Mini-Mental State Examination was used for cognitive functioning assessment, and the score of <24 was considered as having cognitive dysfunction. Cox regression analyses were conducted to derive hazard ratios (HRs) and 95% confidence intervals (CIs) for the effects of various drinking water sources, changes in such sources, and its interaction with exercise on cognition dysfunction. We included 2304 respondents aged 79.67±10.02years; of them, 1084 (44.49%) were men. Our adjusted model revealed that respondents consistently drinking tap water were 21% less likely to experience cognitive dysfunction compared with those drinking untreated water (HR=0.79, 95% CI: 0.70-0.90). Respondents transitioning from natural to tap water showed were 33% less likely to experience cognitive dysfunction (HR=0.67, 95% CI: 0.58-0.78). Moreover, the HR (95% CI) for the interaction between drinking tap water and exercising was 0.86 (0.75-1.00) when compared with that between drinking untreated water and not exercising. All results adjusted for age, occupation, exercise, and body mass index. Prolonged tap water consumption and switching from untreated water to tap water were associated with a decreased risk of cognitive dysfunction in older individuals. Additionally, exercising and drinking tap water was synergistically associated with the low incidence of cognitive dysfunction. These findings demonstrate the importance of prioritizing drinking water health in rural areas, indicating that purified tap water can enhance cognitive function among older adults.

Gestational hypothyroxinemia induces ASD-like phenotypes in behavior, proinflammatory markers, and glutamatergic protein expression in mouse offspring of both sexes.

The prevalence of autism spectrum disorder (ASD) has significantly risen in the past three decades, prompting researchers to explore the potential contributions of environmental factors during pregnancy to ASD development. One such factor of interest is gestational hypothyroxinemia (HTX), a frequent condition in pregnancy associated with cognitive impairments in the offspring. While retrospective human studies have linked gestational HTX to autistic traits, the cellular and molecular mechanisms underlying the development of ASD-like phenotypes remain poorly understood. This study used a mouse model of gestational HTX to evaluate ASD-like phenotypes in the offspring. To induce gestational HTX, pregnant mice were treated with 2-mercapto-1-methylimidazole (MMI), a thyroid hormones synthesis inhibitor, in the tap-drinking water from embryonic days (E) 10 to E14. A separate group received MMI along with a daily subcutaneous injection of T4, while the control group received regular tap water during the entire pregnancy. Female and male offspring underwent assessments for repetitive, anxious, and social behaviors from postnatal day (P) 55 to P64. On P65, mice were euthanized for the evaluation of ASD-related inflammatory markers in blood, spleen, and specific brain regions. Additionally, the expression of glutamatergic proteins (NLGN3 and HOMER1) was analyzed in the prefrontal cortex and hippocampus. The HTX-offspring exhibited anxious-like behavior, a subordinate state, and impaired social interactions. Subsequently, both female and male HTX-offspring displayed elevated proinflammatory cytokines in blood, including IL-1β, IL-6, IL-17A, and TNF-α, while only males showed reduced levels of IL-10. The spleen of HTX-offspring of both sexes showed increased Th17/Treg ratio and M1-like macrophages. In the prefrontal cortex and hippocampus of male HTX-offspring, elevated levels of IL-17A and reduced IL-10 were observed, accompanied by increased expression of hippocampal NLGN3 and HOMER1. All these observations were compared to those observed in the Control-offspring. Notably, the supplementation with T4 during the MMI treatment prevents the development of the observed phenotypes. Correlation analysis revealed an association between maternal T4 levels and specific ASD-like outcomes. This study validates human observations, demonstrating for the first time that gestational HTX induces ASD-like phenotypes in the offspring, highlighting the need of monitoring thyroid function during pregnancy.

전국 먹는 수돗물의 만족도에 대한 결정 요인 분석과 정책적 시사점

This study offers the first analysis of the determinants of overall satisfaction with drinking tap water using a nationally representative sample based on data from the &lt;2021 Drinking Tap Water Survey&gt; in South Korea. Based on a representative sample from 161 regions across the nation, as our main explanatory variables, we select various perceptions concerning consuming tap water including overall quality, taste, being economical, convenience, and environmental-friendliness. When it comes to consumption of tap water with no treatment (inclusive of boiling it), we find that trust in the quality of the tap water is key to improving overall satisfaction with drinking tap water. With reference to consumption of tap water after filtration, the perception of convenience is found to have a statistically significant positive impact. Regardless of how tap water is consumed, the overall satisfaction improves for high income region respondents perceive that drinking tap water is environment-friendly. In addition, other supply-side factors such as trust in the municipal water treatment system and favorable ratings of the speed of response to complaints were associated with higher satisfaction with tap water. These findings have important policy implications.

Domestic access to water in a decentralized truck-to-cistern system: a case study in the Northern Village of Kangiqsualujjuaq, Nunavik (Canada).

Municipal water supply through truck-to-cistern systems is common in northern Canada. Household satisfaction and concerns about water services likely impact user preferences and practices. This case study explores household perspectives and challenges with regard to domestic access to water in a decentralized truck-to-cistern system. A case study was conducted in the Northern Village of Kangiqsualujjuaq, Nunavik (Quebec, Canada). A paper-based questionnaire was completed by 65 households (one quarter of the population). Many households (37%) reported not drinking tap water from the truck-to-cistern system. Chlorine taste was a frequently reported concern, with those households being significantly less likely to drink water directly from the tap (p = 0.002). Similarly, households that reported a water shortage in the previous week (i.e., no water from the tap at least once) (33%) were more likely to express dissatisfaction with delivered water quantity (rs = 0.395, p = 0.004). Interestingly, 77% of households preferred using alternative drinking water sources for drinking purposes, such as public tap at the water treatment plant, natural sources or bottled water. The study underscores the importance of considering household perspectives to mitigate the risks associated with service disruptions and the use of alternative sources for drinking purposes.

Consumption of sucrose in maternal and postnatal stages leads to kidney affectation in adult male rats

High sugar-sweetened beverage intake has been related to human kidney disease and metabolic alterations. We determine the impact of high sucrose intake from pregnancy until early postnatal days and post-weaning on kidneys from adult male offspring rats. Wistar female rats were mated and assigned into two groups: one control drinking tap water (CM) and another drinking 5 % sucrose diluted in water (SM). Two offspring per mother were randomly allocated into two experimental groups at weaning. One had free access to simple water (CO) and the other to 5 % sucrose (SO) for 14 weeks. After treatment, levels of relative aquaporin-2 (AQP2), glomerulosclerosis index (GI), collecting tube area, perirenal fat, blood creatinine, and blood ureic nitrogen concentration (BUN) were determined. Two-way ANOVA followed by Bonferroni post-hoc test was used, considering P ≤ 0.05 as a significant statistical difference. Sucrose consumption during gestation/lactation and interaction increased AQP2 expression in the renal cortex and BUN concentration. In contrast, gestation/lactation consumption increased collecting tube area, post-weaning consumption favored perirenal fat, and finally, gestation/lactation, post-weaning, and the interaction caused glomerulosclerosis. Our results suggest that the consumption of sucrose water during gestation/lactation or post-weaning or combination triggers pathological changes in the kidneys of adult rats.

Consumption of Tap Water and Sociodemographic-Associated Characteristics: A Nationwide Cross-Sectional Study.

Safe water is a global public health concern amid increasing scarcity and pollution. Bottled water production and consumption contribute to these problems. This study examines tap water consumption in Italy, assessing associated sociodemographic factors and related health outcomes such as obesity and self-perceived health status. Data from the Italian National Statistics Institute's "Aspects of daily life" survey (N = 45,597) were analyzed. Covariates included education, age, gender, economic status, region, concerns about waste and climate change, consumption of carbonated drinks excluding water, alcohol consumption, consumption of vegetables, consumption of snacks, body mass index, and self-perceived health status. Bivariate analyses and mixed-effect logistic regression models explored the associations. People who drink tap water made up 19,674, with a higher prevalence in people aged 45 to 59 old, people with a graduate/post-graduate degree diploma, with optimal economic resources, people concerned about waste production and climate change, and those coming from the north-east regions of Italy. Underweight people showed a higher prevalence of TW consumption as well as those who less than occasionally drank carbonated drinks, drank alcohol, consumed vegetables more than once a day and snacks less than once a week, dairy products more than once a day, sweet less than once a week, cured meat less than once a week, and chicken meat less than once a week, those with no consumption of sheep meat, consumption of beef meat less than once a week and consumption of pork meat less than once a week, and those with a satisfactory level of perceived health status. Regressions showed that all other age classes are less likely to drink tap water than people younger than 20 years old. The category with "inadequate" economic resources is more likely to consume tap water. Low educational classes show a low likelihood of consuming tap water as well as islands. A concern about waste production and climate change is associated with an increased likelihood of consuming tap water. Tap water consumption was negatively associated with obesity but not with a satisfactory self-perceived health status. Insights from this study can inform public health strategies.

Effect of bottled fluoridated water to prevent dental caries in primary teeth: study protocol for a phase 2 parallel-group 3.5-year randomized controlled clinical trial (waterBEST)

BackgroundFluoridation of public water systems is known as a safe and effective strategy for preventing dental caries based on evidence from non-randomized studies. Yet 110 million Americans do not have access to a fluoridated public water system and many others do not drink tap water. This article describes the study protocol for the first randomized controlled trial (RCT) of fluoridated water that assesses its potential dental caries preventive efficacy when delivered in bottles.MethodswaterBEST is a phase 2b proof-of-concept, randomized, quadruple-masked, placebo-controlled, parallel-group trial designed to estimate the potential efficacy of fluoridated versus non-fluoridated bottled water to prevent dental caries incidence in the first 4 years of life. Two hundred children living in eastern North Carolina, USA, and aged 2–6 months at screening are being allocated at random in a 1:1 ratio to receive fluoridated (0.7 mg/L F) or non-fluoridated bottled water sourced from two local public water systems. Throughout the 3.5-year intervention, study water is delivered monthly in 5-gallon bottles to each child’s home with instructions to use it whenever the child consumes water as a beverage or in food preparation. Parents are interviewed quarterly to monitor children’s water consumption and health. At annual visits, the presence of dental caries is evaluated with a dental screening examination. Clippings from fingernails and toenails are collected to quantify fluoride content as a biomarker of total fluoride intake. The primary endpoint is the number of primary tooth surfaces decayed, missing, or filled due to dental caries measured by the study dentist near the time of the child’s fourth birthday. Tooth decay is assessed at the threshold of macroscopic enamel loss. For the primary aim, a least-squares, generalized linear model will estimate efficacy and its one-tailed, upper 80% confidence limit.DiscussionwaterBEST is the first evaluation of a randomized intervention of fluoridated drinking water in bottles to prevent dental caries in the primary dentition. This innovative method of delivering fluoridated water has the potential to prevent early childhood caries in a large segment of the US population that currently does not benefit from fluoridated public water.Trial registration ClinicalTrials.gov NCT04893681. Registered on March 2022. Last update posted on 10 October 2023. https://clinicaltrials.gov/study/NCT04893681?cond=Dental%20Caries%20in%20Children&term=fluoride&locStr=North%20Carolina,%20USA&country=United%20States&state=North%20Carolina&distance=50&rank=1

Prevalence of Helicobacter pylori infection among Slovenian children and adolescents: A prospective cohort study.

Helicobacter pylori (H. pylori) infection is primarily acquired in childhood and is notably influenced by socioeconomic variances across different geographical regions. The aim of this study is to assess the prevalence of H. pylori infection in Slovenian children and to identify potential risk factors that facilitate the infection. Between 2019 and 2022, we conducted a multi-center prospective cross-sectional study among healthy children residing in three different administrative regions in Slovenia. H. pylori infection status was determined using a monoclonal antibody-based stool antigen test (SAT). A standardized questionnaire was designed to evaluate the influence of various H. pylori-associated risk factors, including demographics and socioeconomic, housing and sanitation conditions. During the 3-year period, we recruited a total of 421 children and adolescents (age range 2-18 years, mean age 10.29 ± 4.95 years). Overall, 46 (10.9%) were diagnosed with H. pylori infection. No associations were found between H. pylori prevalence rates and increasing age, sex, parental education level, country of birth of the child or their parents, number of household members, household income, having a dishwasher, owning a pet, duration of breastfeeding, fruit intake frequency, drinking tap water, and handwashing practices. The only parameters associated with an increased risk of infection were the location of the school (p < 0.001) and living in an urban area (p = 0.036). The odds of infection were approximately 4.77 times higher if the child attended school in the Central Slovenian compared to other regions (OR = 4.77; 95% CI 0.87-2.34). This is the first study providing information on the prevalence of H. pylori infection among Slovenian children and adolescents. Using SAT, we have shown that the burden of H. pylori infection in our pediatric population is low; however, it seems to depend on regional rather than socioeconomic factors.

Characteristics of Traveler’s Diarrhea in Foreign Traveler’s Visiting Beaches in Badung Regency, Bali

Traveler’s Diarrhea (TD) is a common infectious disease impacting travelers from developed countries who visit developing countries, such as Indonesia. Reporting of Traveler’s Diarrhea (TD) cases in Indonesia is still low, especially in Bali. This study aimed to describe the characteristics of Traveler’s Diarrhea (TD) in foreign travelers visiting beaches in Badung Regency, Bali. This study used cross sectional descriptive method in collecting data. The population was all foreign travelers visiting eight beaches in Badung Regency, Bali. In this study 61 respondents were selected using a purposive sampling technique. Data collection involved a questionnaire and analyzed using univariate statistics. The majority of respondents were European females (70,5%), aged 26-35 (41%), from Germany (11,5%), visiting Bali for tourism (80,3%), their first time in Southeast Asia (47,5%) and Bali (45,9%). Predominant diarrhea characteristics included 3-4 bowel movements per day (75,4%), loose stool consintency (59%) accompanied by abdominal cramps (41%), lasting 1-7 days (96,7%), a single episode (73,8%). Most managed Traveler’s Diarrhea (TD) with medication (63,2%) and continued their travels (80,3%), consumed vegetable salad (50,8%), consumed overnight food (91,8%), consumed raw meat (80,3%) and drank tap water (95,1%). This study suggest that Traveler’s Diarrhea (TD) is prevalent among foreign travelers visiting beaches in Badung Regency, Bali with key factors being the absence of pre-travel consultations, consumption of drinks with ice and vegetables salad. Keywords: Traveler’s Diarrhea, TD, Foreign Traveler, Characteristic

Modeling Quality and Price Perception in the Choice of Drinking Water in France: A Hybrid Choice Model Approach

AbstractThe water resources literature usually discards the important price difference between bottled water and tap water as a predictor of drinking water choice. In France, bottled water is about 100 times more expensive than tap water. Using 4,003 survey responses, we model water resources quality (mis)‐perception and water price (mis)‐perceptions by means of a hybrid choice model. We show that respondents who are more likely to consider the quality of water resources as “very poor” or “poor” are less likely to drink tap water. Furthermore, we find that respondents who do not report the correct price difference between tap water and bottled water are more likely to drink bottled water, which is a novel finding, as significant price effects of this type have never been reported in the literature on drinking water choice.

Feasibility of increasing calcium content of drinking tap water following quality regulations to improve calcium intake at population level

Background Calcium intake is below recommendations in several parts of the world. Improving calcium intake has benefits not only for bone health but also helps to prevent pregnancy hypertension disorders. Calcium concentration of tap water is usually low The aim of the present study was to determine the maximum amount of calcium that can be added to tap water while complying with drinking water local regulations. Methods Tap water samples were collected from the Province of Buenos Aires (Argentina). Physicochemical properties and saturation index were measured. Different incremental concentrations of calcium chloride were added to the experimental aliquots. Results Baseline water had a mean calcium concentration of 22.00 ± 2.54 mg/L, water hardness of 89.9 ± 6.4 mg/L CaCO3, and a saturation index of -1.50 ± 0.11. After the addition of 0.4554 ± 0.0071 g of salt, water hard-ness reached 355.0 ± 7.1 mg/L CaCO3, a calcium concentration of 140.50 ± 2.12 mg/L, and a saturation index -0.53 ± 0.02. Conclusions This study shows that at laboratory level it is feasible to increase calcium concentration of drinking water by adding calcium chloride while complying with national standards. Calcium concentration of drinking tap water could be evaluated and minimum calcium concentration of tap water regulated so as to improve calcium intake in populations with low calcium intake.

Does oral health goal setting during medical visits improve parents' oral health behaviors?

The Rocky Mountain Network for Oral Health (RoMoNOH) promotes the delivery of preventive oral health services (POHS) to children receiving care at community health centers (CHCs) in Arizona, Colorado, Montana, and Wyoming. One POHS is oral health goal setting (OHGS). This study aimed to evaluate the effect of OHGS during medical visits on parent/caregiver-reported oral health behaviors (OHBs). The RoMoNOH implementation team trained CHC healthcare providers in POHS, including caries risk assessment, oral health education, fluoride varnish application, dental referrals, and parent/caregiver oral health engagement. To promote parents' oral health engagement, healthcare providers were trained in motivational interviewing (MI) with OHGS at medical visits. To evaluate the impact of MI with OHGS on parent/caregiver OHBs, a healthcare team member invited parents/caregivers to complete a baseline survey after their medical visits. The evaluation team sent a follow-up survey after 10-14 days. The surveys measured parents/caregivers' goals, confidence in goal attainment, OHBs, and sociodemographics; the follow-up survey also measured OHGS attainment. Improvement in parent/caregiver-reported OHBs was tested with a paired t-test and unadjusted and adjusted multiple linear regression. In total, 426 parents/caregivers completed the baseline survey; 184 completed both surveys. OHBs, including toothbrushing frequency, stopping bed bottles, drinking tap water, and brushing with fluoride toothpaste improved over the evaluation interval. After adjusting for covariates, brushing with fluoride toothpaste (p = 0.01), drinking tap water (p = 0.03), and removing bed bottles (p = 0.03) improved significantly. MI with OHGS with parents/caregivers during medical visits has potential to improve OHBs on behalf of their children.

Ultra-trace analysis of chromium ions (Cr3+/Cr6+) in water sample using selective fluorescence turn-off sensor with natural carbon dots mixed graphene quantum dots nanohybrid composite synthesis by pyrolysis

This study was aimed to implement the one-pot pyrolysis for hybrid nanomaterial synthesis consisting of graphene quantum dots (GQDs) from solid citric acid and carbon dots (CDs) which was hydrothermally derived from the herbal plant extract of “Houttuynia cordata”, denoted as Carbon Dots mixed Graphene Quantum Dots (CDs@GQDs). The nanohybrid were structurally characterized by XPS, FT-IR, Fluorescence, and UV–Visible absorption spectroscopy, and their morphological images were also analyzed by HR-TEM and FE-SEM/EDX. The quantum yield was found to be 15 %, higher than that of intact CDs or GQDs. Upon excitation at a maximum wavelength of 355 nm, and emission in the 450 nm. In this case, it was observed that the nanohybrid exhibit a high degree of selectivity towards Cr6+ ion when employed as a fluorescence quenching sensor from screening test with different metal ions. Therefore, the optimization conditions of solution pH, concentration of nanohybrid, H2O2 concentration as an oxidizing agent, reaction duration time with the aid of ultrasonication were carried out in details and the permissible limit of Cr3+ presence in the target analyte was all undertaken in order to enhance the detection of Cr6+ species. Its linear calibration curve was such y = 0.37x + 55.7, with a coefficient of determination (r2) value of 0.9816. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 17.2 nM, and 52.2 nM. Consequently, the nanohybrid as a green chelating agent in the selective assessment of ultra-trace amount of Cr6+ in spiked water sample model was also done with good recoveries in both bottled drinking water and tap water samples ranging from 102.86 to 114.23 %.

Sucrose consumption modifies the urethrogenital reflex and histological organization of the bulbospongiosus muscle in the male rat

Disorders of the bulbospongiosus muscle (Bsm) are associated with male sexual dysfunction, such as premature ejaculation. We determined the effect of sucrose-water consumption during pregnancy-lactation and postnatal on reflex responses and morphology of Bsm fibers in adult male Wistar rat offspring. Female rats were mated and grouped into consumed tap water mothers and sucrose-water (5 %) mothers during pregnancy-lactation to obtain experimental groups. Male pups were weaned and assigned into four groups (n = 12; each group). Those from control mothers who continued drinking tap water (CM–CO group) or sucrose water (CM–SO group), and those from sucrose mothers who drank tap water (SM–CO group) or continued drinking sucrose water (SM–SO group) until adult life. In male rat offspring (n = 6 per group) was recorded the electrical activity of Bsm was recorded during penile stimulation and urethrogenital reflex (UGR). Other male rat offspring were designated for histological analysis (n = 6 per group). Sucrose consumption during prenatal stages increased the frequency of the Bsm during UGR, while pre and postnatal consumption modified muscle fiber cross-sectional area and increased the collagen content, suggesting that a combination of a diet with pre- and postnatal sucrose changes the Bsm morphophysiology possibly causing male sexual dysfunctions.