India harbors enormous linguistic, ethnic and genetic diversity. Khatries are Indo-European speaking, clan driven community. The present study was an attempt to provide genetic information in the light of anthropological aspects. High resolution HLA typing was performed on 200 unrelated healthy individuals by multiplex bead array system (Luminex-SSO). Statistical analysis was performed by using ‘Arlequin’ software (Version 3.1) package. HLA-A locus illustrated predominant occurrence of A ∗ 11:01, ∗ 01:01, ∗ 03:01 and ∗ 33:03. HLA-A locus diversity is dominated by HLA-A ∗ 02 and A19 allelic groups. Major A ∗ 02 repertoire were A ∗ 02:11 (32.8%), unique to the Indian population and A ∗ 02:01 (31.1%) followed by ∗ 02:03, ∗ 02:05 and ∗ 02:06. The A19 family showed predominant occurrence of A ∗ 33:03 (43.1%) and a unique Indian allele ∗ 33:06. At HLA-B locus, HLA-B5 was highest occurring group, represented by B ∗ 51:01(4.2%) and ∗ 52:01(8.5%). Highest diverse HLA-B allele was B ∗ 15(11.7%) having B ∗ 15:01, ∗ 15:02, ∗ 15:05, ∗ 15:11, ∗ 15:17, ∗ 15:18, ∗ 15:25, ∗ 15:29, and ∗ 15:39 alleles. Only four alleles as ∗ 27:03(evolved in West Africa), ∗ 27:04, ∗ 27:05 and ∗ 27:07 were observed from B ∗ 27 group. Among HLA-C locus, frequently observed alleles were C ∗ 04:01, C ∗ 06:02, C ∗ 07:01 and C ∗ 07:02. At the DR locus, frequently found alleles were DRB1 ∗ 03:01, ∗ 15:01, ∗ 07:01, ∗ 13:01. Six subtypes of DR4 were encountered of which ∗ 04:03 was the predominant one. HLA-DRB1 ∗ 14 (7.4%) allele was represented by ∗ 14:01, ∗ 14:02, ∗ 14:04, ∗ 14:05, ∗ 14:06 and ∗ 14:07subtypes. DR2 alleles also showed greater diversity by the presence of ∗ 15:01, ∗ 15:02, ∗ 15:03 ∗ 15:06, ∗ 15:08, ∗ 15:26, ∗ 16:01 and ∗ 16:02. DQA1 ∗ 05:01, ∗ 01:02, ∗ 01:03, ∗ 02:01 and DQB1 ∗ 02:01, ∗ 06:01, ∗ 06:03, ∗ 02:02 alleles were in tight linkage with DRB1 alleles, Furthermore HLA-A-B-DR haplotypes showed high prevalence of ∗ 26:01- ∗ 08:01- ∗ 03:01, ∗ 02:05- ∗ 50:01- ∗ 03:01, ∗ 33:03- ∗ 44:03- ∗ 07:01 and ∗ 33:03- ∗ 58:01- ∗ 03:01. Major HLA-DRB1-DQA1-DQB1 haplotypes were ∗ 03:01- ∗ 05:01- ∗ 02:01, ∗ 07:01- ∗ 02:01- ∗ 02:02 and ∗ 15:01- ∗ 01:02- ∗ 06:01. The data highlights effect of natural selection, convergent evolution and racial admixture in the population. Our results provide a reference for further anthropological and epidemiological studies.