Downstream Sampling Sites Research Articles

Bacterial community profiles within the water samples of leptospirosis outbreak areas.

Leptospirosis is a water-related zoonotic disease. The disease is primarily transmitted from animals to humans through pathogenic Leptospira bacteria in contaminated water and soil. Rivers have a critical role in Leptospira transmissions, while co-infection potentials with other waterborne bacteria might increase the severity and death risk of the disease. The water samples evaluated in this study were collected from four recreational forest rivers, Sungai Congkak, Sungai Lopo, Hulu Perdik, and Gunung Nuang. The samples were subjected to next-generation sequencing (NGS) for the 16S rRNA and in-depth metagenomic analysis of the bacterial communities. The water samples recorded various bacterial diversity. The samples from the Hulu Perdik and Sungai Lopo downstream sampling sites had a more significant diversity, followed by Sungai Congkak. Conversely, the upstream samples from Gunung Nuang exhibited the lowest bacterial diversity. Proteobacteria, Firmicutes, and Acidobacteria were the dominant phyla detected in downstream areas. Potential pathogenic bacteria belonging to the genera Burkholderiales and Serratia were also identified, raising concerns about co-infection possibilities. Nevertheless, Leptospira pathogenic bacteria were absent from all sites, which is attributable to its limited persistence. The bacteria might also be washed to other locations, contributing to the reduced environmental bacterial load. The present study established the presence of pathogenic bacteria in the river ecosystems assessed. The findings offer valuable insights for designing strategies for preventing pathogenic bacteria environmental contamination and managing leptospirosis co-infections with other human diseases. Furthermore, closely monitoring water sample compositions with diverse approaches, including sentinel programs, wastewater-based epidemiology, and clinical surveillance, enables disease transmission and outbreak early detections. The data also provides valuable information for suitable treatments and long-term strategies for combating infectious diseases.

Vertical and Lateral Variation of Ecological Risk Index of Heavy Metals in the Euphrates River Sediments between Heet and Fallujah, Iraq.

The heavy metals (HMs) contamination of river sediments and their ecological risks is a global environmental problem that has attracted the attention of researchers and those interested in environmental protection. The study aims to investigate the ecological hazards of some HMs in sediments of Euphrates River between the cities of Heet and Fallujah, Iraq and their lateral and vertical variations. At three depths—0-30, 30-60, and 60-90 cm—samples of sediment were collected from five sites. Atomic Absorption Flame Emission Spectrophotometer (ASC.7000) was used to measure concentration of Cd, Cr, Cu, Ni, Pb, and Zn. The ecological risk index (ErI) values of metals in Euphrates River sediment in the study area take the following descending order: Cd > Ni > Cu > Cr > Pb > Zn. The ErI levels were low for Cr, Cu, Ni, Pb and Zn and considerable ecological risk for Cd. The obtained results did not show a common lateral variation pattern of the ecological risk of the measured metals. There is a high level of risk for the measured metals at the downstream sampling sites except for the risk from Cd on the contrary. The potential ecological risk of Cd, Cr, Pb and Zn showed a similar vertical variation pattern which in turn reflects a common source or origin for these metals, while copper and nickel showed a different common distribution pattern.

Geochemical characterization, its controlling factors, and comparison between the upstream and downstream segments of the Himalayan Satluj River basin, India

Satluj River Basin (SRB) has special economic significance, representing the country's most incredible hydro-structure with fresh water, a primary source of irrigation and hydroelectric power in Northern India. Therefore, SRB's geochemical characterization is vital for hydropower and agricultural sustainability. This study provides a preliminary characterization of the hydrogeochemical processes controlling the natural water evolution in SRB by observing the spatial variation and qualitative assessment of solute chemistry (major ions and trace/heavy elements) in the Satluj river, associated tributaries, and groundwater. Hydrochemical characteristics displayed the predominance of geogenic weathering processes with carbonates dissolution and silicate weathering as end members that could explain all the geochemical variability within the SRB. The results reveal that the chemical signature of the upstream segment is possibly influenced more by carbonate weathering than silicate weathering, and the trends reverse in the downstream section with the increasing influence of silicate weathering. Dissolved heavy metals (HMs) such as Mn, Cr, Fe, Co, Ni, Cu, Zn, As, Cd, and Pb showed large fluctuations among upstream and downstream sampling sites, indicating diverse environmental conditions. Human health risk assessment (HHRA) studies revealed less or no potential noncarcinogenic via HMs, except for the downstream stretch of tributaries inferring increased non-cancerous risk. Moreover, the HHRA with HMs (Cr, As, Cd, and Pb) manifested high carcinogenic risks for adults and children across the SRB. The finding of this study could be the baseline dataset for assessing the future status, effective management, and better utilization of water resources of the SRB.

Seasonality can override the effects of anthropogenic activities on microplastic presence in invertebrate deposit feeders in an urban river system

Microplastic pollution is an urgent threat to the biota of aquatic ecosystems and is generally recognized as a global issue. Identifying the sources of microplastics is acknowledged as the most effective approach for mitigating microplastic pollution. However, the factors that regulate the spatiotemporal dynamics of microplastics in urban river networks, such as microplastic sources and other variables, have not been studied together at the watershed scale, let alone regarding their impact on internal microplastics. Here, we define "internal microplastics" as microplastics in biota, either in the digestive system or internal organs of organisms. We estimated the effects of anthropogenic activities (land cover and wastewater treatment plants) and seasonality on the concentration of internal microplastics in midge larvae (Diptera: Chironomidae) in an urban river system at a watershed scale in Taiwan. Agricultural activities, but not industrial activities, had a significant negative nonlinear effect on microplastic concentration. However, seasonality was the most crucial factor, as the microplastic concentration was significantly lower during the wet season. Although the presence of a wastewater treatment plant significantly increased the microplastic concentration at downstream sampling sites, its effect appeared to be minor. We conclude that seasonality overrides the effects of anthropogenic activities on the variation in the concentration of internal microplastics in midge larvae in an urban river system.

Using particle size distributions to fingerprint suspended sediment sources—Evaluation at laboratory and catchment scales

AbstractApplications of sediment source fingerprinting studies are growing globally despite the high costs and workloads associated with the analyses of conventional fingerprint properties on target sediment samples collected using traditional methods. To this end, there is a need to test new fingerprint properties that can overcome these challenges. Sediment particle size could potentially contribute here since it is relatively easy to measure but, until now, has rarely been deployed as a fingerprint itself. Instead, particle size has been used to ensure that source and target sediment samples are more directly comparable on the basis of the fingerprints used. Accordingly, this work examined whether particle size distributions (PSDs) could be used as a reliable fingerprint for apportioning sediment sources, in combination with a grain size un‐mixing model. Application of PSDs as a fingerprint was tested at two scales: (i) in a laboratory setting where soil samples with known PSDs were used to generate artificial mixtures to evaluate un‐mixing model results, and (ii) a catchment setting comparing PSDs in a confluence‐based approach to test if downstream target sediment PSDs could be un‐mixed into the contributions of sediment coming from an upstream and a tributary sampling site. Laboratory results showed that the known proportions of the two, three and four soil samples in the artificial mixtures were predicted accurately using the AnalySize grain size un‐mixing model, giving average absolute errors of 9%, 8% and 6%, respectively. Catchment results showed variable performances when comparing un‐mixing results with sediment budget estimations, with the best results obtained at higher discharge values during storm runoff events. Overall, our results suggest the potential of using PSDs for estimating contributions of sediment sources delivering SS with distinct PSDs when sources are located at short distance to the downstream sampling site.

Ecological quality of the Alibori River, northern Benin, using macroinvertebrate indicators

The Alibori River, which flows through Benin’s cotton crop regions, receives surface water from much of the cultivated land that is situated along its banks. Chemical pollution in surface runoff from this land use threatens the ecological quality of the river. This study aimed to characterise the ecological status of the Alibori River under such agricultural pressures using biological indices and macroinvertebrate metrics. Water and macroinvertebrate samples were taken monthly from fifteen sites along the river between June 2015 and May 2016. The measured physico-chemical parameters and biological indices were subjected to descriptive statistics, bivariate correlations and partial least squares regression (PLSR). Taxonomic richness decreased from the upstream to the downstream reaches of the river. Sampling sites with high mineral content and organic load were home to more pollution-tolerant taxa, such as Chironomidae and Oligochaeta, with a high abundance of Thiaridae. Diversity indices reveal an unbalanced community and macroinvertebrate distribution characterised by the development of opportunistic taxa such as the gastropod Melanoides tuberculata. Decreases in taxonomic composition and community organisation between the upstream and downstream reaches of the river appear to be linked to less stable environmental conditions at the downstream sampling sites, and were compounded with a gradual increase in stress for organisms from the upper to lower reaches of the river. The composition, distribution and diversity characteristics of taxa collected is an indication that the ecological status of the Alibori River is under pressure, as a result of the agricultural activities along its banks.

Reservoir NO3- pollution and chemical weathering: by dual isotopes of δ15N-NO3-, δ18O-NO3- and geochemical constraints.

Reservoir dams alter the nutrient composition and biogeochemical cycle. Thus, dual isotopes of δ18O-NO3- and δ15N-NO-3 and geochemical signatures were employed to study the NO3- pollution and chemical weathering in the Three Gorges Reservoir (TGR), China. This study found that the TGR dam alters the δ15N-NO3- composition and is enriched in the recharge period. Values of δ15N-NO3- varied from 4.5 to 12.9‰ with an average of 9.8‰ in the recharge period, while discharge period δ15N-NO3- ranged from 3.2 to 12.5‰, with an average of 9.3‰. δ18O-NO3- varies (1.2-11.3‰) with an average of 6.5‰ and (2.4-12.4‰) with an average of 7.5‰, in the recharge and discharge periods, respectively. Stable isotopic values sharply decreased fromupstream to downstream, indicating thedamming effects. δ18O-NO3- and δ15N NO3- confirm that sewage effluents, nitrification of soil organic material, and NH4+ fertilizers were the primary sources of NO3- in the reservoir. Carbonate weathering mainly provides ions to the reservoir. HCO3- + SO42- and Ca2+ + Mg2+ represent 90% of major ions in theTGR. Downstream sampling sites showed low solute concentration during the recharge period, indicating the dam effect on solute concentration. Ca-Mg-Cl-, Ca-HCO3- and Ca-Cl- were the main water types in theTGR. The average percentage of solutes contribution revealed the carbonate weathering, evaporites dissolution, silicate weathering, and atmospheric input were 51.9%, 41%, 7.8%, and 1.7% for the recharge period. In contrast, the discharge period contributed 66.4%, 29.2%, 10%, and 4.3%, respectively. TGR water is moderately suitable for irrigation, and hardness is high in drinking water. This study provides new insight into the dual isotopic approach and geochemical signatures to interpret the NO3- cycle and chemical weathering process under dam effects in the TGR. However, this isotopic application has some limitations in source identification, isotope fractionation, and transformation mechanisms of nitrate. Thus, further studies need to be done on these topicsfor a better undestanding.

Heavy metal toxicity, ecological risk assessment, and pollution sources in a hydropower reservoir.

Heavy metal (HM) toxicity, ecological risk, and pollution sources were analyzed using the pollution indexing and statistical methods in the Three Gorges Reservoir (TGR). The average concentration of HM increased in the order of Cr < Ni < As < Cd < Cu < Mn < Pb < Zn < Al < Fe during the recharge period and Cd < Cr < Ni < As < Cu < Pb < Mn < Zn < Al < Fe during the discharge period. Significant spatial variations of Pb, Zn, Cd, As, Mn, Ni, Cr, and Cu were observed at the upstream and downstream sampling sites. Pb sharply increased during the recharge period, ranges (6.93-148.62μg/L) and exceeded WHO and USPEA standards limit. HPI, HEI, Cd, WPI indicated low pollution and moderate pollution with the strong influence of Pb and Cd in the discharge and recharging period, respectively. HTML values are below the permissible toxicity load except for Pb. The Pb toxicity removal percentage is 56.47%, suggesting that the lead's toxicity level is high in TGR and requires the removal process. Ecological risk index values indicated that pollution is low in TGR. The potential ecological risk indexes (RI) of 9.07 and 31.60 were obtained for the discharge and recharge period, respectively, indicating low potential ecological risk from heavy metals in TGR. However, RI values revealed that (Pb, Cd, As Cr Ni, Cu Zn, and Mn) were the most ecological risk HMs in TGR. A significant ecological risk of Pb and Cd distribution was observed across the TGR. Multivariate statistical results found that Pb, Cd, Zn, Mn, Ni, As, Cr, Cu mainly originate from industrial wastewater, mining, metals processing, and agricultural runoff. Fe and Al were mainly from bedrock weathering. Pb, Cd, Zn HMs are a threat to the reservoir ecosystem. This study delivered a current status of HM pollution, toxicity, ecological risk, and pollution sources, indicating a vital insight into HM pollution and water security management inthe Three Gorges Reservoir.

Using stable isotopes and major ions to identify recharge characteristics of the Alpine groundwater-flow dominated Triglavska Bistrica River

Triglavska Bistrica is a typical Alpine river in the north-western part of Slovenia. Its recharge area includes some of the highest peaks in the Julian Alps. The hydrogeological conditions and flow of the river depend largely on groundwater exchange between the karstified aquifer in the carbonate rocks and the intergranular aquifer in the glaciofluvial deposits. The average volume of the river flow is up to several m3/s. In this study, water samples from different locations along the river were analysed for stable isotope ratios of oxygen and hydrogen, major ions, and concentration of tritium activity. The correlation of major ions suggests that the recharge area consists of both limestone and dolomite rocks. The δ18O and δ2H values decrease downstream, implying that the average recharge elevation increases. At the downstream sampling site V-5, located approx. 300 m upstream from the confluence of the Sava Dolinka River, the calculated mean recharge altitude is estimated to be 1,996 m.

Occurrence of indicator bacteria and pathogens in the waters of the noyyal river near its source and downstream in coimbatore district

Water, the bloodstream of the biosphere, also acts as a sink, solvent and transport vehicle for domestic, agricultural and industrial waste, causing pollution. The Noyyal River, a tributary of the River Cauvery, was once the lifeline of Coimbatore, but is now being threatened by pollution from various sources. Assessments of microbiological water quality have focused on the presence of Fecal Indicator Bacteria, which indicate that a contamination pathway exists between a bacterial source and the water supply, serving as a route for pathogen entry. Water samples from seven sites (Kooduthurai, Madakkadu,Thombilipalayam, Alandurai, Mathampatti, Perur and Aathuppalam) along the Noyyal, were analyzed for various microbiological characteristics. The results indicated that the river was relatively unpolluted upstream near its sources, but is progressively polluted on moving further downstream towardsCoimbatore city. Levels of Total Heterotrophic Bacteria, indicators such as Enterobacteriaceae, Total Coliforms, Fecal Coliforms (FC), Fecal Streptococci (FS), pathogens like Salmonella sp., Shigella sp., and Vibrio sp. were elevated at downstream sampling sites. Evaluation of the FC:FS ratio revealed that, the water was unfit for drinking at almost all the downstream sampling stations, and could have limited use for irrigation. The release of fecal microorganisms and pathogens from the sediments to the water column could be a major source of bacterial loading. Non-point sources were identified as the major contributors of contaminants. Regular monitoring of the river water and sediment, amelioration of point / non-pointsources of pollution, and speedy implementation of remedial measures, may restore this stretch of the Noyyal River to its former pristine state.

Assessment of spatial and temporal variations in water quality by the application of multivariate statistical methods in the Kali River, Uttar Pradesh, India.

The Kali River is a significant source of surface water as well as the main tributary of River Hindon that flows through major cities of western Uttar Pradesh, India. It flows throughout the urban and industrial regions; hence, it carries various amounts of pollutant. Therefore, a study was conducted to examine spatial-temporal variations in river water quality by determining physicochemical variables and heavy metal concentrations at seventeen sampling stations (S1-S17) throughout the river stretch. Various physicochemical variables, namely pH, EC, TDS, turbidity, BOD, COD, TH, TA, Ca, Mg, Na, K, HCO3-, Cl-, SO42-, NO3-, and PO43- were higher in summer than in winter. The order of mean metal concentrations was Fe > Pb > Mn > Ni > Zn > Cu > Cr > Cd. The relationships among measured physicochemical variables and pollution index were examined. Furthermore, multivariate statistical methods were used to assess spatial-temporal variation in water quality to identify current pollution sources and validate results. Water quality index and comprehensive pollution index indicated that the Kali River was less polluted from S1 to S8. However, downstream sampling sites were polluted. Pollution starts from S9 and drastically increases at and beyond S13 because of effluents from industries and sugar mills in Muzaffarnagar. The study suggests cleaning the downstream region of river to restore human health and flora and fauna in the river ecosystem.

Metal Contamination of Water and Sediments of the Vieira River, Montes Claros, Brazil.

Vieira River is the main recipient of domestic and industrial wastewater in the city of Montes Claros, MG, Brazil. Until 2010, domestic sewage was dumped in it without any kind of treatment. Concentrations of arsenic (As), chrome (Cr), copper (Cu), nickel (Ni), lead (Pb), and zinc (Zn) were determined in water and sediment samples in eight locations along the Vieira River during the dry season of 2015. Concentrations of Cu, Ni, and Zn detected in the water at some sites along the Vieira River were superior to the reference limits for toxicity. The concentration of Cu and Ni restricts the use of water for irrigation in some sites of the river. The level of sediment contamination was assessed by five approaches, including contamination factor (CF), pollution load index (PLI), geo-accumulation index (Igeo), cluster analysis (CA), and principal component analysis/factor analysis (PCA/FA). The results showed that Cr and the downstream sampling site nearest to the Wastewater Treatment Plant of the city of Montes Claros had the highest values of PLI, Igeo, and CF, which reinforces the influence of domestic and industrial wastewater discharge in pollution of the Vieira River. In addition, CA and PCA/FA reinforced the assumption that Cr comes from anthropogenic pollution sources.

&lt;p class="HeadingRunIn"&gt;&lt;strong&gt;Monthly Diversity and Abundance of Caddisflies in Upstream and Downstream Areas of the Mae Ngat Somboonchol Dam, Chiang Mai Province, Thailand&lt;/strong&gt;&lt;/p&gt;

The Mae Ngat Somboonchol Dam, located in Chiang Mai Province of northern Thailand, was constructed to serve the purposes of irrigation and electricity generation for the region. This hydroelectric dam is located on the Mae Ngat River, which is a tributary of the Ping River. The aim of this experiment was to study the diversity and abundance of the population of caddisflies in this river. Seven sampling sites, including 2 upstream and 5 downstream sites, were monitored monthly for adult male caddisflies using a light pan trap device over 1 year (June 2013 – May 2014). A total of 3,844 male specimens belonging to 16 families and 94 species were caught. However, the total abundance of caddisflies was higher at the upstream sampling sites than at the downstream sampling sites. The top three most abundant species found to be present in this river were Oecetis scutulata MARTYNOV, 1936, Setodes argentiguttatus GORDON &amp; SCHMID, 1987 and Cheumatopsyche globosa ULMER, 1910, respectively. The species compositions of the upstream and downstream sites differed. 44 specimens of the species Trichoptera were represented exclusively at the upstream sites. Examples of this include Macrostemum fenestratum ALBARDA, 1887, Setodes endymion MALICKY &amp; CHANTARAMONGKOL, 2000, Hydropsyche camillus MALICKY &amp; CHANTARAMONGKOL, 2000 and Ecnomus jojachin MALICKY &amp; CHANTARAMONGKOL, 1993. The 23 species exclusively represented in the downstream sites included Aethaloptera sexpunctata KOLENATI, 1859, Cheumatopsyche banksi MOSELY, 1942, Oecetis tripunctata FABRICIUS, 1793 and Ecnomus votticius MALICKY &amp; CHANTARAMONGKOL, 1993. There were 50 newly recorded species while 18 species were absent when compared to a previous study from 2009. The diversity and abundance also differed during each month of collection. The highest numbers of trapped male caddisflies were found from April to May, which were 352 and 292, respectively. The upstream sites contained a higher number of caddisflies and a greater level of diversity than the downstream sites.

Sediment Fingerprinting to Delineate Sources of Sediment in the Agricultural and Forested Smith Creek Watershed, Virginia, USA

AbstractThe sediment fingerprinting approach was used to apportion fine‐grained sediment to cropland, pasture, forests, and streambanks in the agricultural and forested Smith Creek, watershed, Virginia. Smith Creek is a showcase study area in the Chesapeake Bay watershed, where management actions to reduce nutrients and sediment are being monitored. Analyses of suspended sediment at the downstream and upstream sampling sites indicated streambanks were the major source of sediment (76% downstream and 70% upstream). Current management strategies proposed to reduce sediment loadings for Smith Creek do not target streambanks as a source of sediment, whereas the results of this study indicate that management strategies to reduce sediment loads in Smith Creek may be effective if directed toward managing streambank erosion. The results of this study also highlight the utility of sediment fingerprinting as a management tool to identify sediment sources.

Variations in concentrations and bioavailability of heavy metals in rivers caused by water conservancy projects: Insights from water regulation of the Xiaolangdi Reservoir in the Yellow River

Water regulation of the Xiaolangdi Reservoir of the Yellow River was chosen as a case to investigate variations in concentrations and bioavailability of heavy metals caused by water conservancy projects in rivers. Water and suspended sediment (SPS) samples were collected at downstream sampling sites along the river during this period. Concentrations and speciation of Zn, Cr, Cu, Ni, and Pb in water and SPS samples were analyzed, and their bioaccumulation was studied with Daphnia magna. This study indicated that the exchangeable and carbonate-bound fractions of heavy metals in SPS decreased along the studied stretch, and the dissolved heavy metal concentrations increased along the river with 1.6–15 folds. This is because sediment resuspension increased along the river during water regulation, giving rise to the increase of heavy metal release from SPS. The dissolved Zn, Cu, Ni, and Pb concentrations were significantly positively correlated with SPS concentrations, and their increase along the river was greater than Cr. The body burdens of heavy metals in D. magna exposed into samples collected from the reservoir outlet were 1.3–3.0 times lower than those from downstream stations, suggesting that the heavy metal bioavailability increased during water regulation. This should be considered in the reservoir operation.

A Multi-season Investigation of Microbial Extracellular Enzyme Activities in Two Temperate Coastal North Carolina Rivers: Evidence of Spatial but Not Seasonal Patterns.

Riverine systems are important sites for the production, transport, and transformation of organic matter. Much of the organic matter processing is carried out by heterotrophic microbial communities, whose activities may be spatially and temporally variable. In an effort to capture and evaluate some of this variability, we sampled four sites—two upstream and two downstream—at each of two North Carolina rivers (the Neuse River and the Tar-Pamlico River) ca. twelve times over a time period of 20 months from 2010 to 2012. At all of the sites and dates, we measured the activities of extracellular enzymes used to hydrolyze polysaccharides and peptides, and thus to initiate heterotrophic carbon processing. We additionally measured bacterial abundance, bacterial production, phosphatase activities, and dissolved organic carbon (DOC) concentrations. Concurrent collection of physical data (stream flow, temperature, salinity, dissolved oxygen) enabled us to explore possible connections between physiochemical parameters and microbial activities throughout this time period. The two rivers, both of which drain into Pamlico Sound, differed somewhat in microbial activities and characteristics: the Tar-Pamlico River showed higher β-glucosidase and phosphatase activities, and frequently had higher peptidase activities at the lower reaches, than the Neuse River. The lower reaches of the Neuse River, however, had much higher DOC concentrations than any site in the Tar River. Both rivers showed activities of a broad range of polysaccharide hydrolases through all stations and seasons, suggesting that the microbial communities are well-equipped to access enzymatically a broad range of substrates. Considerable temporal and spatial variability in microbial activities was evident, variability that was not closely related to factors such as temperature and season. However, Hurricane Irene's passage through North Carolina coincided with higher concentrations of DOC at the downstream sampling sites of both rivers. This DOC maximum persisted into the month following the hurricane, when it continued to stimulate bacterial protein production and phosphatase activity in the Neuse River, but not in the Tar-Pamlico River. Microbial community activities are related to a complex array of factors, whose interactions vary considerably with time and space.

Metal accumulation in sediments and amphipods downstream of combined sewer overflows

The aim of this study was to investigate the concentrations of the metals Ag, Cd, Cu, Mn, Mo, Pb, Pt and Zn in sediments and amphipods along a 100m transect downstream of three different combined sewer overflows (CSOs). Moreover, the study was implemented to better understand the biological availability of metals downstream of CSOs as well as to identify a potential risk of CSOs for benthic or pelagic biota. Samples were taken at downstream sampling sites, which were 1, 5, 10, 20, 50 and 100m away from the outlets of the CSOs. An upstream-located sampling site each was used as reference for the respective transect. Additionally, sediments in two retention zones, located between the CSOs and the receiving creeks were analyzed. All downstream located creek sediments showed a similar metal pattern. Metal concentrations were found to be highest within the first 20m of the creek sediments. Elevated metal concentrations were also detected in the sediments of the retention zones. Metal accumulation in the amphipods was different at all three locations, but highest levels were always found downstream of the sediment accumulation hot spots. This might indicate that the metals were remobilized and became available for the amphipods further downstream of the CSOs.Although the general contamination pattern with respect to each reference site was similar, the degree of contamination was different at each location as demonstrated by anthropogenic enrichment factors. The discharge frequency as well as anthropogenic influences from upper reaches appear to be the most important factors for high metal concentrations in the sediments. Accordingly, in one case high background concentrations in combination with the CSO lead to an exceedance of the predicted effect level of Zn for aquatic organisms. Therefore, sediment contamination should be included in risk assessment when constructing CSOs to protect aquatic life.

Presence of Emerging Per- and Polyfluoroalkyl Substances (PFASs) in River and Drinking Water near a Fluorochemical Production Plant in the Netherlands.

The present study investigated the presence of legacy and emerging per- and polyfluoroalkyl substances (PFASs) in river water collected in 2016 up- and downstream from a fluorochemical production plant, as well as in river water from control sites, in The Netherlands. Additionally, drinking water samples were collected from municipalities in the vicinity from the production plant, as well as in other regions in The Netherlands. The PFOA replacement chemical GenX was detected at all downstream river sampling sites with the highest concentration (812 ng/L) at the first sampling location downstream from the production plant, which was 13 times higher than concentrations of sum perfluoroalkylcarboxylic acids and perfluoroalkanesulfonates (∑PFCA+∑PFSA). Using high resolution mass spectrometry, 11 polyfluoroalkyl acids belonging to the C2nH2nF2nO2, C2nH2n+2F2nSO4 or C2n+1H2nF2n+4SO4 homologue series were detected, but only in downstream water samples. These emerging PFASs followed a similar distribution as GenX among the downstream sampling sites, suggesting the production plant as the source. Polyfluoroalkyl sulfonates (C2nH2F4nSO3) were detected in all collected river water samples, and therefore appear to be ubiquitous contaminants in Dutch rivers. GenX was also detected in drinking water collected from 3 out of 4 municipalities in the vicinity of the production plant, with highest concentration at 11 ng/L. Drinking water containing the highest level of GenX also contained two C2nH2nF2nO2 homologues.

Ecosystem-Based Management strategies to improve aquaculture in developing countries: Case study of Marismas Nacionales

The Ecosystem Based Management approach is an opportunity to maximize ecosystem services while promoting ecological resilience and appropriate productive activities. However, implementing this management strategy relies on acknowledging spatial interactions between economic activities and ecosystem services as well as an effective communication between scientists and decision-makers. The lack in understanding those interactions and management decisions based on limited information has led to an unsuccessful transition between artisanal and intensive aquaculture in a Wetland of International Importance (NW Mexican Pacific). In this study, we evaluated the effect of aquaculture as a driver of wetland degradation and water quality and analysed the perceptions of decision makers and scientists concerning the spatial scale of aquaculture impacts on ecosystem services.From 1997–2013, we found that herbaceous wetland and croplands have been lost while grasslands, urban areas, aquaculture and mangroves have increased. Shrimp ponds were built on natural water bodies, herbaceous wetlands and croplands. However, perturbed mangrove patches are found near aquaculture ponds, mostly downstream. Water samples were analysed and differences were found between upstream and downstream sampling sites in the concentration of DO, COD, TS, salinity, SO42-, Cl-1, CaCO3, Na, Cr, Ni and Al. Sediment analysis showed that the amount of coarse sediment such as sand and gravel diminished significantly upstream and does not reach the river mouth. Finally, perceived spatial relationship analyses showed that most academics surveyed recognized that aquaculture could have a far-reaching impact outside the immediate production area. On the other hand, more than half of the decision makers who responded did not perceive any impact and the majority of the rest considered that the impact occurred close to the ponds. The likelihood of integrated coastal management strategies being implemented depends on the close collaboration of scientists and decision makers, on their understanding of complex spatial interactions, on developing appropriate management alternatives and on incorporating science in spatial planning and decision making.

Molecular Genetics Informs Spatial Segregation of Two Desert Stream Gila Species

AbstractHeadwater Chub Gila nigra and Roundtail Chub G. robusta are two distinct but morphologically similar cyprinid species that historically were parapatric in Fossil Creek (Gila River basin) in central Arizona. The creek was chemically treated in 2005 to eradicate nonnative fishes, and chubs salvaged before the treatment were repatriated afterward. Annual samples from multiple sites along a transect throughout the treated reach were characterized for mtDNA and microsatellites from 2008 to 2014. Genetic variation was compared within and among Fossil Creek samples and with reference material for Headwater Chub from Fossil Springs at the headwaters of the creek and for Roundtail Chub from the Verde River and West Clear Creek. Measures of mtDNA and microsatellite diversity identified significant differences among Headwater Chub from Fossil Springs, Roundtail Chub from the Verde River, and samples above and below Irving Falls, a natural fish barrier within the treatment reach. The frequency of Headwater Chub mtDNA was low at the most downstream sampling sites, progressively increasing upstream. There was also temporal variation, with Headwater Chub mtDNA generally becoming rarer in later samples. F‐statistic analysis of microsatellite data and Bayesian clustering of microsatellite genotypes with STRUCTURE were generally consistent and identified three groups: Headwater Chub and two forms of Roundtail Chub. Using the species assignments provided by STRUCTURE and mitotypes, we found 153 “pure” Headwater Chub and only a single pure Roundtail Chub above Irving Falls and 27 pure Headwater Chub and 398 pure Roundtail Chub below the falls. Tests of cytonuclear disequilibrium indicated nonrandom mating (with the rare species more likely to mate than the more common one) and/or selection against hybrids. These data support the recognition of the Headwater Chub as a distinct full species and demonstrate that conservation actions within the genus Gila should typically be carried out at the population level.Received April 8, 2016; accepted August 16, 2016 Published online December 2, 2016