Double Stage Research Articles

Full-Scale Gear Tooth Bending Fatigue Tests Obtained Early in the Development of a Rotorcraft Transmission

A new gear testing method is introduced to reduce development cost and time. It allows component-level testing of individual gear meshes and new gear designs. Instrumented gear sets are tested at full load while the rest of gearbox components are still being built. Since the fatigue strength of the gears is determined earlier in the development cycle, design deficiencies are identified and understood earlier. In this new method, an individual gear mesh installed in a stiff facility housing is used to mimic the contact pattern and bending stress demonstrated by the same mesh in an actual aircraft housing. Analytical gear models are used to identify the displacement difference between the stiff test facility and the aircraft housing. The test stand is designed so it can be adjusted accurately to provide gear and pinion positions that are representative of the deflected positions under load in the aircraft housing. A spiral bevel mesh and a split torque double helical reduction stage with multiple meshes are evaluated using the developed method. The contact pattern and strain survey results of the gear meshes are correlated with predicted results.

Influence of a double-stage solar tunnel dryer on the preservation of quality characteristics and the Modelling of colour variations in red chili peppers

In this work, the influence of two-stage solar tunnel drying on the preservation of quality attributes of both chili pepper varieties, Mareko Fana (MF) and Bako Local (BL), was studied. Both varieties were dried in different drying modalities, i.e. solar tunnel and open sun drying. The quality attributes were evaluated during the drying process using FTIR and spectrophotometry techniques. Different colour evolution models were implemented and a suitable model was selected. The results showed that after drying of the Mareko Fana chili variety, the lightness retention was found in the range of 81 %–89 %, the redness retention between 25 % and 42 % and the yellowness retention between 14 % and 38 %. For the Bako Local variety, the values were in the range of 78 %–82 %, 42 %–60 % and 36 %–55 %, respectively. The first order model gave a higher correlation coefficient (R2 > 0.96), which indicated the suitability of the model for predicting the color variation for both varieties. The volatile aromatic compounds in the chili peppers were greatly lost during open sun drying, while solar tunnel drying maintained these compounds for both varieties. However, during the solar tunnel drying process, a significant amount of alkyl halides or alkyl-chlorides were lost. The amount of dihydrocapsaicin and capsaicin from the chili peppers ranged from 10,172 μg/kg to 16,313 μg/kg and 16,676 μg/kg to 27,189 μg/kg for both dihydrocapsaicin and capsaicin, respectively. In tunnel, open-air, and uncontrolled open-air solar drying, the MF chili variety lost copsaicinoid content by 3.4 %, 14.8 %, and 38.3 %, respectively; in the BL variety, comparable losses were 1.8 %, 4.4 %, and 13.6 %. A minimum loss of ascorbic acids was recorded during solar tunnel drying. The results showed that well-designed double stage solar tunnel dryers are important for effective drying processes that preserve quality attributes of the chili pepper.

Impact of vegetation albedo on the habitability of Earth-like exoplanets

ABSTRACT Vegetation can modify the planetary surface albedo via the Charney mechanism, as plants are usually darker than the bare surface of the continents. We updated ESTM (Earth-like surface temperature model) to incorporate the presence, distribution and evolution of two dynamically competing vegetation types that resemble grasslands and trees (the latter in the double stages of life: adults and seedlings). The newly developed model was applied to estimate how the climate-vegetation system reaches equilibrium across different rocky planetary configurations, and to assess its impact on temperature and habitability. With respect to a world with bare granite continents, the effect of vegetation-albedo feedback is to increase the average surface temperature. Since grasses and trees exhibit different albedos, they affect temperature to different degrees. The ultimate impact on climate depends on the outcome of the competition between these vegetation types. The change in albedo due to vegetation extends the habitable zone and enhances the overall planetary habitability beyond its traditional outer edge. This effect is especially relevant for planets that have a larger extension of continents than Earth. For Earth, the semimajor axis d = 1.04 au represents the turning point where vegetation enhances habitability from h = 0.0 to 0.485 (in the grass-dominance case), to h = 0.584 (in the case of coexistence between grasses and trees), and to h = 0.612 (in the tree-dominance case). This illustrates the transition from a snowball state to a planet with intermediate habitability at the outer edge of the circumstellar habitability zone.

Double subduction controls on long-lived continental tectonics and subcontinental mantle temperatures

The complexities of convergent margins commonly include the interactions of subduction zones, with many geological records of “double” subduction. Here, we build two-dimensional numerical models to explore the evolution of complex subduction systems by systematically testing single and inward-dipping double subduction beneath a continental upper plate and the impact of continental collision on these systems. When compared to single subduction models, the inward-dipping double subduction shows hindered trench migrations and larger volumes of upwelling mantle enhanced by excess sinking slab mass. Double subduction draws larger volumes of hotter mantle beneath the continent in an area much broader than the marginal basins of single subductions, contributing to subcontinental heating by ∼200 °C. As collision jams one margin of a double subduction system, the other margin follows the evolution of migrating single subduction zones, although characterized by persisting higher mantle temperatures and strong upwellings, inherited from the double subduction stage, and large-scale upper plate extension. The modeling outcomes are compared to scaling arguments to test the viability of the mechanism proposed for tectonics of the Cenozoic South China Sea and Neoproterozoic Yangtze Block (southeastern China), where the inward-dipping double subduction provides a context for protracted large-scale continental extension, hotter subcontinental temperatures, and channeled mantle flow not easily reconciled with the dynamics of single subduction zones.

Estimation of the Risk of Hearing Loss in Neonates with Hyperbilirubinemia in the Mysuru District Using Double Stage Double Screening Method

Estimation of the Risk of Hearing Loss in Neonates with Hyperbilirubinemia in the Mysuru District Using Double Stage Double Screening Method

Advanced control of double stage grid-tied three phase photovoltaic systems with shunt active power filter

This paper explores the challenges associated with the control of a two-stage three-phase electrical grid connected to a photovoltaic (PV) system. The objectives encompass: i) maximizing the available PV power, ii) controlling the DC-link voltage to a predetermined setpoint, and iii) considering that power quality has become an important measure in a distribution electrical network where different loads are connected, the third objective will mainly focus on ensuring power factor correction (PFC). To achieve these objectives, two loops of nonlinear controller are developed. In the outer loop, the duty cycle of a boost converter is controlled using a hybrid technique of backstepping technique and the perturb & observe (P&O) algorithm. In addition, the inner loop employs a hybrid automaton approach to tackle the challenges of a three-phase shunt active power filter (SAPF). The results have been verified through numerical simulation using MATLAB/Simulink power systems environment.

P-763 Twin births in Belgium: a national cohort analysis of 35 523 double fresh embryo transfers between 2012 and 2021

Abstract Study question Should a national embryo transfer (ET) policy designed in the era of cleavage stage transfers be adapted for blastocyst transfers to avoid twin pregnancies? Summary answer Double embryo transfer policy results in unacceptable high twin rates at all ages and treatment ranks which is significantly more pronounced after double blastocyst transfer. What is known already In 2003, authorities in Belgium implemented a law to reduce multiple pregnancies after ART by tailoring ET policies based on age, embryo quality, and cycle rank. A choice between SET or Double Embryo Transfer (DET) depending on embryo quality is allowed in the second cycle for patients under 36y with SET being mandatory with top quality embryos. DET is allowed as of the first cycle for patients aged 36y or more regardless of embryo quality. Blastocyst transfer results in significantly higher live birth rates, implying an increased risk of multiple pregnancies after multiple blastocyst transfers. Study design, size, duration A analysis of 35,523 double embryo fresh transfer cycles over a period of 10 years from 2012 to 2021 from the Belgian national BELRAP (Belgian Registry on Assisted Procreation) registry was performed. The study included 26,930 double cleavage stage transfers (DET-CL) and 8,593 double blastocyst transfers (DET-BL). Participants/materials, setting, methods The primary outcomes were delivery rate (DR) per transfer and twin delivery rate (TDR). DR includes all deliveries with a least one live birth per transfer; the TDR includes all twin deliveries as a proportion of all deliveries. For analysis, the data were categorized into the following age groups: 18-25 years, 26-29 years, 30-35 years, 36-39 years and 40-45 years. Cycle ranks were categorized as follows: rank 1, rank 2 and cycle 3- ≥ 6. Main results and the role of chance For all age groups and cycle ranks, delivery rate per transfer and twin delivery rate per delivery were significantly higher after DET-Bl compared to DET-Cl (26.0% versus 19.9%; p < 0.0001 and 28.0% versus 20.6%; p < 0.0001, respectively). In the age groups 26-29 years, 30-35 years and 36-39 years, a significantly higher TDR was observed in DET-Bl compared to DET-Cl, respectively (34.4% versus 24.2%, p = 0.0005; 33.5% versus 24.9%, p < 0.0001 and 24.3% versus 17.3%, p = 0.0002). TDR after DET-Bl was significantly lower in women aged 40-45 years compared to all other age groups, 18-25 years, 26-29 years, 30-35 years and 36-39 years, respectively (11.3% versus 32.8%, p < 0.0001; 11.3% versus 34.4%; p < 0.0001; 11.3% versus 33.5%; p < 0.0001 and 11.3% versus 24.3%; p < 0.0001), respectively). DR was significantly lower in cycle rank 1 compared to cycle rank 2 (22.5% versus 25.5%; p < 0.0001) and compared to cycle rank 3 - > 6 rank 1 and (22.5% versus 27.1%; p < 0.0001). After DET-Bl, a significantly lower TDR was observed in cycle rank 1 compared to cycle rank 2 (15.6% versus 30.0%; p < 0.0001) and compared to cycle rank 3 - > 6 (15.6% versus 29.7%; p < 0.0001). Limitations, reasons for caution The retrospective design is a limitation. Outcomes might be influenced by clinical and laboratory improvements over the years. The registry does not collect embryo quality data making it impossible to assess the effect of embryo quality on the ET policy and outcome. Wider implications of the findings This data indicates that a double blastocyst transfer should only be considered from the age of 40 onwards. In the first cycle, regardless of age, we observe a significantly lower twin delivery rate, suggesting that a double ET is performed when only poor-quality blastocysts are available. Trial registration number Not applicable

Solid state microdosimetry of a 148 MeV proton spread-out Bragg peak with a pixelated silicon telescope

A constant value of the Relative Biological Effectiveness (RBE), equal to 1.1, to weight the physical dose of proton therapy treatment planning collides with the experimental evidence of an increase of effectiveness along the depth dose profile, especially at the end of the particle range. In this context, it is desirable to develop new optimized treatment planning systems that account for a variable RBE when weighting the physical dose. In particular, due to the increasing interest on microdosimetry as a possible methodology for measuring physical quantities correlated with the biological effectiveness of the therapeutic beam, the development of new Tissue-Equivalent Proportional Counters (TEPCs) specifically designed for the clinical environment are in progress.In this framework, the silicon technology allows to produce solid state detectors of real micrometric dimensions. This is a valid alternative to the TEPC from a practical point of view, being simple, easy-of-use and more versatile. The feasibility of a solid state microdosimeter based on a monolithic double stage silicon telescope has been previously proposed and deeply investigated by comparing its response to the one obtained by reference TEPCs in various radiation fields. The device is constituted by a matrix of cylindrical elements, 2 μm in thickness and 9 μm in diameter, coupled to a single E stage, 500 μm in thickness. Each segmented ΔE stage acts as a solid state microdosimeter, while the E stage gives information on the energy of the impinging proton up to about 8 MeV.This work is dedicated to the description of the microdosimetric characterization of the 148 MeV energy-modulated proton beam at the radiobiological research line of the Trento Proton Therapy Centre by means of a pixelated silicon microdosimeter. All measurements were carried out at different positions across the spread-out Bragg peak (SOBP) and the corresponding microdosimetric distributions were derived by applying a novel extrapolation algorithm. Finally, microdosimetric assessment of Relative Biological Effectiveness was carried out by weighting the dose distribution of the lineal energy with the Loncol's biological weighting function. Benefits and possible limitations of this approach are discussed.

A New CRITIC-GRA Model for Stope Dimension Optimization Considering Open Stoping Stability, Mining Capacity and Costs

In the long-hole stage open stoping with subsequent backfill mining of underground metal mines, the selection or optimization of stope dimension parameters is significant for safe and economic mining operations. To analyze the optimal stope sizes, the Mathews empirical graph method and FLAC3D numerical method can be used, but the analyzed safety results of the two methods are generally independent from each other. More importantly, economic indicators including production capacity and mining costs should be considered simultaneously to optimize the stope dimension which was mostly ignored in previous reports. In this paper, a new CRITIC-GRA model was proposed for the first time to build up a multi-factor quantitative optimization for stope dimension, which allows for a comprehensive analysis with preset influential safety and economic indicators. The indicators considered include the safety indicators such as stability probability for the side walls and roof of the open stope via the updated Mathews graph method, maximum displacement, plastic zones volume and maximum principal stress via FLAC3D simulations, as well as economic indicators such as mining costs and stope production capacity in mine operations. The model was then illustrated in an underground iron mine. With the given rockmass quality in the mine, the overall stability of the open stope can be improved instead of reduced to enlarge the single stage stope height (60 m) to a double stage height (120 m) by reducing the stope width from 20 m to 15 m, thereby significantly increasing the mineable ore amount and improving the stope safety. An integrated evaluation of open stope stability, mining capacity and costs objectively determined that scheme No. 10, with a slope length of 50 m, a width of 15 m and a height of 120 m, was the optimum out of the 20 preset schemes. The new CRITIC-GRA model offers a dependable reference tool for determining the optimal stope dimensions in similar underground mines.

Split flow principle implementation for advanced subcritical double stage organic rankine cycle configuration for geothermal power production

The main goal of research is to find the most suitable combination of working fluids, for double stage Organic Rankine Cycle configurations which ensure maximum net power output for low to medium enthalpy geothermal sources with temperatures from 120 °C to 180 °C. All obtained results are compared with the values obtained in simple Organic Rankine Cycle configuration. In general, it can be concluded that the highest net power output is produced by the double stage Organic Rankine Cycle configuration in the geothermal temperature interval from 120 °C to 132 °C. In the geothermal fluid inlet temperature interval from 143 °C to 180 °C double stage Organic Rankine Cycle configuration achieves the highest net power output values at 150 °C, 160 °C, 170 °C and 180 °C and that 40,93 (kW) with a combination of working fluids R236fa/R1234yf (high temperature stage/low temperature stage), 49,85 (kW) with R236ea/R227ea, 59,03 (kW) with R236ea/R227ea and 69,40 (kW) with n-Butane/R1234ze(E). In the temperature interval from 132 °C to 142 °C all considered configurations achieve similar values of net power output.

Multi-scale modelling of an electrodialysis with bipolar membranes pilot plant and economic evaluation of its potential

Sodium hydroxide and hydrochloric acid are widely used chemicals in different industrial sectors. To minimize costs and risks associated with transportation, handling and storage, these hazardous chemicals can be produced in situ employing electrodialysis with bipolar membranes (EDBM). This work presents a multi-scale model capable of simulating large scale EDBM units with complex stack configuration (i.e., internal staging) that can be used to design and optimize the process. The model was validated in two different process configurations using experimental results obtained from an EDBM pilot plant. Discrepancies between model and experimental results in the range of 2–11 % were obtained. The validated model was used to conduct a techno-economic evaluation adopting the feed and bleed configuration. Results show that current efficiency increases as the current density rises. At 600 A m−2, values of current efficiency between 72 % and 96 % were found for sodium hydroxide concentration in the range of 0.5–1 mol L−1. The levelized cost of sodium hydroxide (LCoNaOH) was evaluated, in the same range of concentrations, demonstrating that values between 280 and 370 € ton−1 can be obtained, fixing the electricity prince (0.1 kWh kg−1) and the triplet specific cost (600 US$ m−2). Moreover, assuming a reduced cost of electricity and triplet to 0.05 kWh kg−1 and 300 US$ m−2, respectively, an absolute minimum of 140 € ton−1 was found for the target 0.5 mol L−1. A double stage EDBM configuration was simulated to show the scale-up potentials of the multi-scale model. A reduction in the LcoNaOH of 10 % was obtained for a target concentration of 1 mol L−1. These results prove the attractiveness of the EDBM technology for producing in situ chemicals.

Effectiveness study of recrystallisation method in pharmaceutical salt production from processed salt with zero waste concept

Indonesia's vast archipelago offers abundant seawater resources, holding the potential for salt production. Salt, a vital commodity in human life, typically contains sodium chloride and impurities like Ca2+, Mg2+, SO42−, and K+. Pharmaceutical salt is an industrial category adhering to pharmacopoeial standards regarding sodium chloride levels and impurity content, ensuring quality for drug preparations in Indonesia. Prior research indicates that recrystallisation, specifically evaporation crystallisation, enhances salt quality by increasing NaCl content. Chemical precipitating agents like NaOH and Na2CO3 can be introduced to improve salt purity further. This study aims to identify optimal conditions for pharmaceutical salt production from processed salt raw materials, considering crystallisation time, stirring speed, chemical additives (NaOH and Na2CO3), and double crystallisation stages. The method commences with pre-treatment, involving salt dissolution in distilled water to saturation, with the addition of precipitating agents as per designated variables. Precipitates formed from precipitating agents (NaOH and Na2CO3) are isolated through filtration. The filtrate undergoes evaporation crystallisation at 103 °C, varying between single and double crystallisation. Salt crystals are separated, dried, and weighed to calculate yield. Pharmaceutical salt is analysed for water content, NaCl, and impurities (Ca2+, Mg2+, SO42−, and K+). The optimal conditions for pharmaceutical salt production were double crystallisation with a 20 % excess of chemicals (NaOH and Na2CO3), 100 min of crystallisation time, and a stirring speed of 600 rpm. This yielded a 15 % NaCl content of 99.87 %, Mg2+ at 0 ppm, Ca2+ at 69.6 ppm, SO42− at 366 ppm, K+ at 370 ppm, and water content at 0.166 %. Notably, the pharmaceutical salt production process generates no waste, as byproducts like Mg(OH)2 and CaCO3 can be recycled and hold commercial value. However, it is essential to re-evaluate raw materials and technologies to address the market's high cost and competitiveness issues.

Energy efficiency analysis of novel combined open absorption multifunctional heat pump and FlashME desalination system with a compressed air dryer

Energy intensive thermal desalination and compressed air-drying processes coupled with low grade heat recovery units can significantly contribute to carbon and energy footprint reduction. In this paper, a novel combined system based on an open absorption multifunctional heat pump and FlashME desalination system with a compressed air dryer is proposed to recover the latent heat from flue gas and efficiently utilize it to drive the desalination process for seawater treatment and supply the compressed dry air. The energy performance of the combined system is analyzed under the effect of various key operational parameters by simulating the validated process model. The results of energy analysis indicate that the novel system can recover the latent waste heat from flue gas with an efficiency of 83.02 % and supply the compressed dry air at low humidity of 1.41 g/kg by operating at a thermal COP of 1.95. Furthermore, the system can efficiently utilize the recovered low-grade heat to produce freshwater at a recovery rate of 23.14 %. The findings of the critical performance analysis show that the drying, and latent heat recovery performance of the coupled system is dependent mainly on the inlet parameters of the spray solution and the flue gas humidity. However, the water recovery of FlashME is totally dependent on heat capacity and the regeneration pressure such that the performance ratio increases from 1.51 to 4.03 by operating with hot seawater of 60–80 °C, when the regeneration pressure is raised up to 53.48 kPa. Moreover, the coupled system configured in parallel double stage can lower 11.43–37.78 % regeneration load along with 54.24 % improvement in distillate productivity when the operating pressure is raised up to 800 kPa and the rise in airflow rate in dryer can decrease the 10.41–52.45 % regeneration load on external heat source.

Microstructural and textural evolution of double stage cold rolled non-grain oriented electrical steel

Non-grain oriented electrical steels (NGOES) are typically used in electric applications with rotating magnetic fields and thus essential for the production of electric motors. The increasing number of electric vehicles results in a growing demand for electrical steel sheets used in the stacked laminations of rotor and stator components. E-mobility applications require the best possible magnetic properties for optimum performance and efficiency, especially at high frequencies. In addition, increased mechanical strength is necessary to withstand the centrifugal forces resulting from high revolution speeds. Chemical composition, microstructure and crystallographic texture strongly influence the magnetic properties, whereas the mechanical properties are mainly affected by the chemical composition and grain size. Optimizing the texture is a possible method for improving the magnetic properties without significantly compromising the mechanical strength. In this work a special processing route for the production of 3.25% Si NGOES sample material has been studied, using laboratory cold rolling and annealing facilities. Compared to the classical processing route of single stage cold rolling and annealing, this alternative method involves two cold rolling steps with an intermediate annealing treatment between the first and second rolling sequence and a final annealing treatment after the second rolling step. The samples were produced with different intermediate thicknesses, changing the amount of cold rolling deformation of each sample variation. The degree of deformation affects the recrystallization and grain growth behavior and thus the resulting microstructure and texture. The microstructural and textural evolution was investigated in the hot rolled, intermediate annealed and final annealed state using electron backscatter diffraction (EBSD). Finished samples were additionally examined using magnetic testing and tensile testing. The results indicate a correlation between the intermediate thickness and the final grain size of the steel samples. Furthermore, the highly grain size dependent mechanical strength and magnetic losses are influenced. EBSD-data were used to interpret the texture and to calculate a parameter describing the magnetic quality of the texture. The results show a strong improvement of magnetically favorable texture components along with increasing intermediate thickness, enabling significantly better polarization values in rolling direction and transversal direction. However, anisotropy of the magnetic properties increases as well due to the textural changes.

Steel strip surface defect detection based on multiscale feature sensing and adaptive feature fusion

A surface defect detection method for hot-rolled steel strips was proposed to address the challenges of detecting small target defects, significant differences in morphology, and unclear defect characteristics. This method is based on multiscale feature perception and adaptive feature fusion. First, based on the spatial distribution characteristics of the steel strip image, redundant background interference is removed using automatic gamma correction and Otsu thresholding. Second, based on the characteristics of surface defects in steel strips, this paper proposes TDB-YOLO (YOLO with a small target detection layer), a Bidirectional Feature Pyramid Network (BiFPN), and Double Cross Stage Partial (CSP) Bottleneck with three convolutions (DC3). To detect small object defects, a small target detection layer with a smaller receptive field focuses on fine-grained features, reducing the model’s probability of missed detection. In terms of feature extraction, DC3 enhances the interaction of feature information from different spatial scales, enabling the model to effectively handle features of varying scales. In terms of feature fusion, the BiFPN is used to adaptively fuse deep-level and shallow-level feature information, enhancing the semantic richness of the feature information. Ultimately, the proposed model in this paper achieved an accuracy of 90.3% and a recall rate of 88.0% for surface defects in steel strips. The mean average precision was 90.4%, and the frames per second was 33. The detection performance of this model outperformed those of other detection models, demonstrating its ability to effectively meet the real-time detection requirements of surface defects in industrial scenarios on steel strips.

Production of Methane and Hydrogen from Mild-Strength Industrial Wastewater

Over the past two decades, fossil fuel reserves have sharply shrunk, which has resulted in a global fall in energy sources. Alternative carbon-neutral renewable energy sources have caught the interest of experts due to the erratic nature of energy prices and their severe ecological effects. The treatment plant’s mild-strength effluent from an equalization tank at an industrial factory was used in this study to generate hydrogen and methane. This mild-strength effluent had a chemical oxygen demand (COD) content of 2.8 ± 1.0 g COD/L. A double stage anaerobic digestion system’s hydrogen-generating digester was run at a hydraulic retention time of 8 h while the methane-generating digester was run at a hydraulic retention time of 24 h. The highest methane production rate (MPR), methane yield (MY), methane content and COD elimination were, in order, 71 ± 30 mL/L-d, 57 ± 10 mL/g COD, 90 ± 1% and 77%, respectively. In this experiment, energy efficiency was assessed using the following criteria: Maximum heating was at a value of 2.1 × 108 kcal/y. Utilizing energy-equivalent coal, natural gas, or fuel reduced annual carbon emissions by 8.7 × 104 kg CO2/y, 5.1 × 104 kg CO2/y and 7.1 × 104 kg CO2/y, respectively.

Abstract 11: Development of innovative off-the-shelf cancer immunotherapies with iPSC-derived CD8-positive T-cells

Abstract Chimeric antigen receptor (CAR) based T-cell immunotherapies have transformed the oncology landscape and evolved as part of the standard of care for several hematological malignancies. To generate CAR-T-cell products, autologous patient-derived αβT-cells are engineered with a CAR for tumor cell targeting. However, generating autologous T-cell products fails in a significant number of cases due to the poor quality and quantity of blood-derived T-cells and is associated with manufacturing and logistical complexity as well as high product costs. Therefore, it is crucial to develop strategies for off-the-shelf T-cell products as otherwise many patients are left without this treatment option. Manufacturing allogeneic T-cells from induced pluripotent stem cells (iPSCs) has a great potential to generate cell products with consistently high quality and scalable quantities. In addition, high fidelity gene-editing can be performed in iPSCs to address tumor resistance mechanisms and to prevent exhaustion of the T-cells. We have established a feeder-free differentiation protocol that enables robust production of iPSC-derived αβT-cells (iαβT). Flow cytometry and single cell transcriptome analysis ensure stringent monitoring of all process stages. To demonstrate functional activity of iαβT, cytotoxicity and cytokine release assays were performed. We compared the differentiation potential of iPSC lines derived from T-cells (t-iPSCs) to a hematopoietic stem cell-derived iPSC line with an αβTCR knock-in. Hematopoietic progenitor cells were induced from the different iPSC lines and differentiated into iαβT. During the differentiation process, surface markers CD45, CD5 and CD7 were displayed, and cells started to express αβTCRs. Importantly, iαβT derived from the different iPSC lines expressed CD8α and CD8β which is crucial for the function of cytotoxic T-cells. The CD4/CD8 double positive stage is an important step in T-cell development and was observed only when T-cells were generated from t-iPSC, but not from the TCR knock-in cells. Functional analysis of iαβT-cells confirmed their cytotoxic activity and potential to release cytokines after activation. Our scalable process for iαβT differentiation generates CD8-positive T-cells that secrete cytokines and show cytotoxic activity against tumor cells, demonstrating their potential as a promising cell source for TCR-T or CAR-T cancer immunotherapies. Citation Format: Riga Tawo, Michela Mirenda, Olivia Cypris, Philip Hublitz, Nadja Wagner, Michael Epstein, Michael Esquerre, Audrey Holtzinger, Monika Braun, Markus Dangl, Rodrigo Grandy, Daniel Sommermeyer. Development of innovative off-the-shelf cancer immunotherapies with iPSC-derived CD8-positive T-cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 11.

Dissecting the molecular basis of spike traits by integrating gene regulatory networks and genetic variation in wheat

Spike architecture influences both grain weight and grain number per spike, which are the two major components of grain yield in bread wheat (Triticum aestivum L.). However, the complex wheat genome and the influence of various environmental factors pose challenges in mapping the causal genes that affect spike traits. Here, we systematically identified genes involved in spike trait formation by integrating information on genomic variation and gene regulatory networks controlling young spike development in wheat. We identified 170 loci that are responsible for variations in spike length, spikelet number per spike, and grain number per spike through genome-wide association study and meta-QTL analyses. We constructed gene regulatory networks for young inflorescences at the double ridge stage and the floret primordium stage, in which the spikelet meristem and the floret meristem are predominant, respectively, by integrating transcriptome, histone modification, chromatin accessibility, eQTL, and protein–protein interactome data. From these networks, we identified 169 hub genes located in 76 of the 170 QTL regions whose polymorphisms are significantly associated with variation in spike traits. The functions of TaZF-B1, VRT-B2, and TaSPL15-A/D in establishment of wheat spike architecture were verified. This study provides valuable molecular resources for understanding spike traits and demonstrates that combining genetic analysis and developmental regulatory networks is a robust approach for dissection of complex traits.

Contribution to the comparison of conventional concentric magnetic gear and double stage concentric magnetic gear for high power offshore wind applications

Nowadays, the replacement of mechanical technologies by magnetic technologies has several advantages. Therefore, in this paper, we compare in an indirect drive chain the conventional concentric magnetic gear (CCMG) and the double-stage concentric magnetic gear (DSCMG) used as a speed multiplier for a high-power offshore wind turbine. This comparison is performed for the same gear ratio and the same torque at the input of both magnetic gears to obtain the same torque values at the output of each gear. The goal is to determine which one has the smaller amount of magnet and the higher volumetric torque density. After the calculation of the gear ratio, a first choice of geometrical parameters is adopted. Several simulations carried out by the finite element method (FEM) allowed to obtain the desired torques and to fix the final geometrical parameters of each magnetic gear. The results obtained show that the DSCMG has both the smallest magnet volume and the highest volumetric torque density compared to the CCMG.

A Double Stage DC/DC Converter for LED Lighting Automotive Systems

In this paper a DC/DC converter, conceived to be employed for LED lighting in automotive application, is proposed. It is composed of two stages to fit both to the constraint to be powered by a variable supply voltage and to deliver a constant output load current with reduced ripple to supply the LED. Firstly the LED has been experimentally characterized to obtain a model to be implemented on a circuit simulator. Then the design of the converter has been carried out, including the control laws, to verify the compliance with the constraints. Finally, a circuit based on commercial devices is proposed to be applied both to lighting and to direction indicators light, including current and temperature protection. Results, given in simulation, show good performance achievable with a low-cost hardware.