An optical analysis is developed to separate forward light scatter of the human eye from the conventional wavefront aberrations in a double pass optical system. To quantify the separate contributions made by these micro- and macro-aberrations, respectively, to the spot image blur in the Shark-Hartmann aberrometer, we develop a metric called radial variance for spot blur. We prove an additivity property for radial variance that allows us to distinguish between spot blurs from macro-aberrations and micro-aberrations. When the method is applied to tear break-up in the human eye, we find that micro-aberrations in the second pass accounts for about 87% of the double pass image blur in the Shack-Hartmann wavefront aberrometer under our experimental conditions.
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