In this paper, we have used the double exposure digital holographic interferometry (DEDHI) technique to determine the diffusion coefficient of urea in pure distilled water at 27oC (room temperature) and it is used further to predict urea movement in soil by considering the concentration of urea solution and diffusion coefficient to study early plant growth. We have recorded several consecutive digital holograms of diffusing solutions of urea and pure distilled water at different times instantaneously on the CCD chip and processed them with H-Digital numerical reconstruction software on the computer. It is observed that as the concentration of urea solution increases from 0 to 4 M, diffusion coefficient 'D' of urea slightly increases from 12.2 to 13.8 × 10^-6 cm^2/s, which considerably affects the growth of the plants.