Doses Of Ovaprim Research Articles

Different dosages effectiveness of Ovaprim on the maturation of freshwater lobster (Cherax quadricarinatus)

Freshwater lobsters have been developed as a commodity for consumption since 2003. The need for this commodity is increasing but production is still very low, so the price of freshwater lobsters is quite high and expensive. Therefore, it is necessary to carry out reproduction to carry out the seeding process for Freshwater Lobsters (LAT) using an injection technique in the form of the Ovaprim Hormone to accelerate the maturity of the LAT gonads. This study aims to examine the administration of different doses of the ovaprim hormone on the maturation of freshwater lobster broodstock. The research was conducted for 60 days. The method used was a Randomized Block Design (RAK) with 4 treatments and 3 replications. The treatments in this study were P0 (control), P1 (0,3 ml), P2 (0,5 ml) and P3 (0,7 ml). The parameters observed in this study were Gonad Maturity Index (IKG), Egg Diameter and Pleopode Fecundity. The results of the observations showed that giving different doses of ovaprim to the maturation of freshwater crayfish was not significantly different (P<0.05) to the Gonad Maturity Index IKG), Egg Diameter and Pleopode Fecundity but was significantly different to the P0 (control) treatment. The best treatment in this study was treatment P3 (0.5 ,l) because it had the highest performance between the other treatments.Keywords: egg diameter; fecundity; freshwater lobster; maturation; ovaprim

Induced breeding, embryonic and larval development of Koi carp (Cyprinus carpio) in Khulna, Bangladesh

The aim of the present study was to perform induced breeding and to observe embryonic and larval development stages in different seasons for mature male and female Koi carp, Cyprinus carpio, was done by administering the synthetic hormone ovaprim, in a single dose for males and a double dose for females at 0.4-1.0 ml/kg body weight via an intramuscular injection. Spawning occurred between 5-6 hrs after the 2nd injection in summer (April) at a temperature of 26-29 oC and 17-20 0C in winter (December). Using doses of 0.4ml/kg, 0.7ml/kg and 1.0 ml/kg of ovaprim, the fertilization rate of eggs were obtained at 42.31%, 54.55% and 61.82 % in summer and 26.92%, 38.00% and 39.23 % in winter. Hatching rates were 42.78%, 44.60% and 55.00 % in the summer season and 30.22%, 31.01% and 31.67 % in the winter season with the same doses of ovaprim. The fertilized eggs were adhesive, transparent, and spherical with diameters ranging between 0.8 mm and 1.0 mm and yellowish white in color. Due to a positive response to the hormone Ovaprim, considerable fertilization and hatching rate, short embryonic period and larval development, it is possible to conduct a successful breeding program of this species commercially

CONSERVATION OF AN ENDANGERED CARNIVOROUS FISH RITA RITA THROUGH INDUCED BREEDING

In present study the Effects of different doses of ovaprim on induced spawning activities of Rita rita were observed with an aim to standardize the dose of ovaprim for successful breeding. In the trial experiment, the females of Rita rita were treated with ovaprim at the rate of 0.5, 0.8 and 1.0ml/kg body weight and males of Rita rita in all the cases were treated at the rate of 0.4ml/kg. The doses of ovaprim at the rates of 0.5, 0.8, 1.0 ml/kg body weight resulted in 0%, 100% and 100% ovulation respectively. In case of injection of ovaprim at the rate of 0.8ml/kg, the fertilization rate was observed to be about 70% and hatching rate was 58%, which occurred within 20-25 hours after fertilization at water temperature of 270C-290C. In case of injection of ovaprim at the rate of 1.0ml/kg, 18% fertilization rate and 5% hatching rate was observed. The doses of ovaprim at the rates of 0.8ml/kg and 0.4ml/kg body weight for female and male Rita rita respectively was found to be satisfactory for overall breeding performance and the commercially successful production of this fish. Hatchlings from over all experiment were reared up to fingerlings size to about 2 month and were finally stocked into the Chashma lake.

Studies on induce breeding performance of magur (Clarias batrachus) in FRP hatchery installed at Nalbari district, Assam

A study was conducted to evaluate the effect of Ovaprim on induced breeding of Magur (Clarias batrachus) in FRP hatcheries H-1, H-2 and H-3 installed in three farms of Nalbari district, Assam for survival of magur larvae. The Ovaprim hormone was found to be efficient in breeding operations of the hatcheries in dose combinations as 1.35±0.15, 1.95±0.15 and 1.16±0.17 used in H-1, H-2 and H-3 respectively. It was obvious that the average relative fecundity, body weight and ovulation percentage in H-2 hatchery were higher compared to H-1 and H-3 which is mainly due to proper feeding and nutrition. The highest % normal larvae was observed in H2 hatchery which was 67.72±13.01 which is due to the effect of different doses of Ovaprim during induced breeding of Magur. The study revealed that FRP hatcheries can be a potential tool for induced breeding and quality seed production of Magur.

Comparative study on the effect of ovaprim and Wova-FH on induced breeding response onAnabas testudineus (Bloch)

The present investigation was carried out for comparative evaluation of two commonly used synthetic hormones ovaprim and Wova-FH on induced breeding response of Anabas testudineus (Bloch). Both the hormones are administered at a rate of 0.2, 0.3 and 0.4 ml/kg body weight of the fish to find out their effect on breeding response, latency period, quantity of eggs released, fertilization and hatching rate. The study reveals that, both the inducing agents administered at a rate of 0.4 ml/kg body weight to Anabas testudineus resulted with complete spawning. The fertilization and hatching percentages were reported to be more when brooders are administered with ovaprim than that of Wova-FH. The study further indicates a strong correlation to exist between the doses of ovaprim with egg output (+0.99), fertilization rate (+0.99), hatching rate (+0.99), spawn production (+0.99).Anegative correlation was reported to exist between the doses of ovaprim with latency period (-0.99) and hatching time (-0.90) which indicates that, with increase in the concentration of ovaprim the latency period and hatching time decreases.

Evaluation of Ovaprim and Human Chorionic Gonadotropin Doses on Spawning Induction and Egg and Larval Quality of Pigfish, Orthopristis chrysoptera

AbstractPigfish, Orthopristis chrysoptera, readily undergo vitellogenesis in captivity, but final oocyte maturation (FOM), ovulation, and spawning can be unpredictable. The objectives of this investigation were to assess the effects of various doses of Ovaprim® (sGnRHa [salmon gonadotropin‐releasing hormone analog] + domperidone) and human chorionic gonadotropin (HCG) on FOM, ovulation, and spawning in pigfish. Ovaprim dosages investigated were 0.25, 0.50, 1.00, and 2.00 mL/kg, which were injected into the dorsal musculature of female pigfish. Male pigfish received one half the dosage administered to females to ensure spermiation. HCG doses of 500, 1000, 2000, and 4000 IU/kg were administered to female pigfish, with male pigfish once again receiving half the female dosage. Eggs were collected over a 72‐h period postinjection and enumerated and assessed for fertilization, hatching percentage, and survival to first feeding. Eggs and larvae were photographed and a suite of morphological parameters was determined. Hormone dose evaluations with pigfish showed that the low‐dose (0.25 and 0.50 mL/kg) Ovaprim treatments resulted in better spawning performance. However, superior egg and larval characteristics validate the 0.50 mL/kg dose as the preferred choice for use with pigfish. The four doses of HCG administered to pigfish performed unreliably in this study; thus, no dose recommendation could be made.

Efektifitas ovaprim terhadap lama waktu pemijahan, daya tetas telur dan sintasan larva ikan lele dumbo, Clarias gariepinus

Abstract This study aimed to determine the effect of different doses of ovaprim on spawning time, egg hatchability, and survival of African catfish larvae (Clarias gariepinus). The research was done in the Central Local Fish Seed (BBI) Jayapura, Papua Province. Experimental design used was Randomized Complete Design. The study consisted of four treatments namely 0 ml, 0.3 ml, 0.6 ml and 0.9 ml ovaprim, each with three replications. Data collected were spawning time, egg hatchability, and survival rate of African catfish larvae. The number of containers used was as much as 12 buckets (for spawning, hatching, and observation of survival rate of larvae). The results showed that treatment with different doses of ovaprim hormones provided a significant influence on the latency time of spawning, egg hatchability and survival rate of larval. The dose of 0.3 ml ovaprim caould increase the latency time of spawning, egg hatchability and survival rate of African catfish larvae. The average latency time of hatching was 552 minutes, spawning and egg hatchability 84.16% and survival rate of living larvae was 85.76%. Keywords : African catfish, Ovaprim, spawning time, egg hatchability, survival rate

Evaluasi Kualitas, Kuantitas Telur Dan Larva Ikan Patin Siam (Pangasianodon Hiphopthalmus) Dengan Penambahan Ovaprim Dosis Berbeda

This study was conducted to determine the effect of different doses of ovaprim on latency time of spawning, hatching rate, and survival of fish larvae (Pangasianodon hiphopthalmus). Experimental design used was Randomized Complete Design (CRD) with four treatments, each with three replication. The treatments including 0 ml, 0.3 ml, 0.6 ml and 0.9 ml ovaprim per kg of body weight of fish. Data collected consisted of latency time of spawning, hatching rate, and survival rate of larvae. The results showed that treatment with different doses of ovaprim hormones resulted in a significant influence on latency time of spawning, egg hatchability and survival rate of larvae. It was found that 0.6 ml ovaprim could increase the latency time of spawning, egg hatchability and survival rate of larvae. The average latency time of larvae was 529 minutes, hatching rate was 80,86% and survival rate of larvae was 83,33%.

Evaluation of Ovaprim and human chorionic gonadotropin doses on spawning induction and egg and larval quality of pinfish, Lagodon rhomboides

The use of exogenous hormones to induce final oocyte maturation (FOM), ovulation, and spawning has become commonplace in the reproductive protocols for many species. Dose efficacy can be highly variable among species and factors such as temperature and stress may further obfuscate the hormone's perceived efficacy. The pinfish, Lagodon rhomboides, is a marine baitfish species with great potential for commercial development. This species readily undergoes vitellogenesis in captivity, however progression through FOM is unreliable. Therefore, the objectives of this investigation were to assess the effects of various doses of Ovaprim® (sGnRHa+domperidone) and human chorionic gonadotropin (HCG) on ovulation and spawning in pinfish.Two experiments were conducted to determine efficacious doses of Ovaprim and HCG in pinfish. Ovaprim dosages investigated were 0.25, 0.50, 1.00, and 2.00mL/kg, injected into the dorsal musculature of female pinfish. Male pinfish received one half the dosage (0.125, 0.25, 0.50, and 1.00mL/kg) administered to females to ensure spermiation. Experimental HCG doses of 500, 1000, 2000, and 4000IU/kg were administered to female pinfish with male pinfish once again receiving half the dosage (250, 500, 1000, and 2000IU/kg). Spawned eggs were collected over a 72hour period post injection and enumerated and assessed for fertilization, hatching percentage, and survival to first feeding. Additionally, eggs and larvae were photographed and a suite of morphological parameters was evaluated.Doses of 0.25 and 0.50mL/kg Ovaprim were efficient at induction of FOM and spawning in pinfish. Superior fecundity, fertilization and 3day post hatch (DPH) survival rates, as well as larger 3 DPH larvae support lower Ovaprim doses as the preferred choice for use with this species. Conversely, the 4000IU/kg HCG dose performed the best of all HCG doses evaluated eliciting the greatest spawning frequency from female pinfish.

Controlled Breeding of Asian Catfish Clarias batrachus using Pituitary Gland Extracts and Ovaprim at different Temperatures, Latency Periods and their Early Development

Present study was conducted on induced breeding of commercially important Clarias batrachus due to the non-availability of its quality seeds from the natural resources for environmental degradation, shrinkage of natural breeding ground and illegal killing of juveniles and brood fishes. The aim of the study was to achieve success in fertilization and hatching using various doses of different inducing agents at different temperatures and latency periods followed by stripping method. In this study the developmental stages of fish (fertilized egg to 45th day old fish) were characterized chronologically. A trial was also made to optimize the survival rate of young developing fish upto 45th day by manipulating their feeding schedule and environmental conditions. The breeding experiments were done with pituitary gland extracts (40 and 120 mg/kg. body weight for female and 25 and 50 mg/kg. body weight for male) and Ovaprim (0.8 and 2.0 ml/kg body weight for female and 0.4 and 1.0 ml/kg body weight for male) at 26o, 28o and 30oC. The highest rates of fertilization (80%) and hatching (71%) of eggs were recorded in Clarias batrachus injected with carp pituitary gland extracts @ 50 mg/kg body weight of male and 120 mg/kg body weight of female at 28oC with a latency period of 15 hours. The fertilization and hatching rates were 77% and 65% respectively at 28°C at the higher doses of Ovaprim. The highest survival rate (82.5%) of developing fish was achieved supplying zooplankton as live feed upto 12th day followed by alternate supply of zooplankton, boiled egg with vitamin C and chopped tubifex from 13th to 45th day of rearing in indoor polyvinyl chloride tray with minimum fluctuation in temperature and dissolved oxygen.

An Economically Viable Approach for Induced Breeding of Labeo Rohita by Ovatide, Ovaprim And Carp Pituitary Extract.

Indian major carp Labeo rohita was induced breeding in eco-carp hatchery with 3 different inducing agents, viz. ovaprim, ovatide, Carp pituitary extract at fish breeding center at Amloh Distt. Fathegarh Sahib (Punjab) India. Total ten trial doses of ovaprim, ovatide and Carp Pituitary Extract (CPE) used for induced breeding in Labeo rohita. The fertilization was found 61.30% with ovaprim, 58.50% with ovatide and 55.96% with carp pituitary extract treatment. The percentage hatchling was 72.20% with ovaprim, 66.37 % with ovatide and 59.25% with carp pituitary extract treatment.A comparison was carried out for fecundity, fertilization, and hatching rate during the induced spawning of Labeo rohita administered single dose of ovaprim and ovatide while carp pituitary extract double dose to female brooder. Ovaprim performed much better than Ovatide and pituitary gland extract.

Breeding and Seed Production of <i>Labeo gonius</i> (Hamilton) under Captive Condition in Manipur

Induced breeding of the Indian medium carp, Kuri (Labeo gonius) (Ham.) was conducted using synthetic hormone, Ovaprim at three dose levels of 0.7, 0.5 and 0.3 ml/kg to female and 0.3, 0.2 and 0.1 ml/kg to male body weight, respectively. The brooders were injected and left to spawn in the breeding hapa at a sex ratio of 2:1 (male: female). It was found that all the doses of Ovaprim could induce the fishes to breed, whereas no breeding was observed in control set. The latency period was found to be 8-9 hours. The egg output/female was highest at a dose of 0.5 ml/kg of female and 0.2 ml/kg of male body weight. The relative fecundity of the species ranged between 90-110 eggs/gram body weight of female. Fertilization and hatching rate was found to be 89.3±2.50 and 85.2±2.30%, respectively with Ovaprim dose of 0.5 ml/kg of female and 0.2 ml/kg of male body weight.

The effects of various doses of ovaprim on reproductive performance of the African catfish <i>Clarias gariepinus</i> (Burchell) and <i>Heterobranchus longifilis</i> (Valenciennes)

Artificial spawning of two African Catfish species viz: C. gariepinus and H. longifilis of 0.18 – 0.64kg and 0.53 – 1.63 kg respectively were carried out using various doses of Ovaprim with carp pituitary extract (C.P.E.) as the control. Oocyte maturation and ovulation were successfully effected with Ovaprim doses of 0.2, 0.25, 0.30, 0.35, 0.40 and 0.50ml/kg for C. gariepinus and 0.30, 0.35, 0.40, 0.45, 0.50ml/kg for H. longifilis. Latency period was between 8 – 11.5hrs at 26.3ºC – 28.0ºC but was indirectly influenced by temperature, species and type of hormone. Hatching was between 22 – 30 hrs at a temperature range of 25.45 – 26.1ºC and was independent of hormone dosage. Percentage deformed try was relatively low and ranged from 0.16 ± 0.28 – 1.08 ± 0.36 in C. gariepinus and 0.52 ± 0.59 . 1.50 ± 0.73 in H. longifilis. Fry survival rate was high and ranged from 89.44 ± 0.27 to 93.47 ± 2.18 in C. gariepinus and 78.57 ± 11.12 to 90.7 ± 3.90 in H. longifilis. Physico-chemical parameters were within the desired range for catfish larval rearing in all the treatments. AJAZEB Vol. 7 2005: pp. 101-105