The impacts of submerged macrophytes on kinetics of alkaline phosphatase were studied in two 680 m 2 enclosures in a shallow Chinese freshwater lake (Donghu Lake) from April to October 1996, and two experimental pools (120 m 2) built inland in 1998. The submerged macrophytes were Vallisneria sp, Potamogeton crispus. In the presence of macrophytes, the concentration of orthophosphate was significantly lower, coupled with the decreasing function of organic P hydrolysis, in terms of lower V max and higher K m values of alkaline phosphatase in water, filtered and unfiltered (0.45 μm); in the interstitial water, the V max values of the enzyme in sediments were significantly lower, exhibited by a spatial and vertical profile. The results implied the key role of submerged macrophytes was the retention of P nutrients.