Dominican Amber Research Articles

The first firefly fossil (Coleoptera: Lampyridae) from Dominican amber

Fireflies (Coleoptera: Lampyridae) comprise ~2,500 extant species worldwide (Ferreira et al., 2020). Considering the extant diversity of firefly species, their fossil record is scant, with only eight taxa across four subfamilies: Luciolinae, Ototretinae, Lamprohizinae, and Lampyrinae (Supplementary file 1; Kazantsev, 2024). Dominican amber may have the best quality of preservation among fossil resins, with a rich diversity of fossil specimens (Grimaldi & Engel, 2005) from the early Miocene (20–15 Ma) (Iturralde-Vinent & MacPhee, 2019). However, no firefly taxon was previously known from this deposit. Here, we describe the first Lampyridae fossil from Dominican amber. It represents a new species of the extant Neotropical genus Lychnacris Motschulsky, 1853, which has 19 extant species known from the island of Hispaniola in the Caribbean (Kazantsev & Perez-Gelabert, 2009, 2013; Perez-Gelabert, 2011).

Pheidole praehistorica sp. nov., a new addition to spiny ants of the genus Pheidole Westwood, 1839 (Formicidae, Myrmicinae) from Oligo–Miocene Mexican amber

A fossil species of ant, Pheidole praehistorica sp. nov. (Hymenoptera, Formicidae, Myrmicinae), is described and illustrated from the late Oligocene and early Miocene amber-bearing beds of Mexico. The type material comprises five amber inclusions from the Simojovel site in Chiapas. Pheidole praehistorica is recognized by having compound eyes positioned mesad on the head and protruded laterally from the head’s margins; pronotum long, forming a neck, with a pair of spines directed dorsolaterally; mesonotal groove deep forming a more or less U-shape concavity in lateral view; dorsal and declivitous faces of the propodeum well differentiated, comprising an approximately 90 degrees angle; propodeum with a pair of long spines; peduncle of the petiole long, as long as the spines of the propodeum. A phylogenetic analysis was also performed using Formica integroides, Camponotus chartifex, Dolichoderus spurius, Cephalotes minutus and Atta mexicana to assess the relationships of P. praehistorica with its fossil spiny congeners from Dominican amber and closely related extant species. The results show close morphological and phylogenetic affinities between the fossil ant P. praehistorica with P. primigenia and P. tethepa from Dominican amber, which may suggest that the shared spinescence character in Pheidole has a probable New World ancestry. The new record of P. praehistorica in the Oligo–Miocene strata of southernmost North America provides further evidence for the ancient distribution of spiny ants of the genus Pheidole in the New World.

Plastome phylogenomics and new fossil evidence from Dominican amber shed light on the evolutionary history of the Neotropical fern genus Pecluma.

Molecular studies based on chloroplast markers have questioned the monophyly of the fern genus Pecluma (Polypodioideae, Polypodiaceae), which has several species of Polypodium nested within it. We explored the delimitation of Pecluma and its biogeographic pattern by evaluating the phylogenetic position of four Polypodium species not sequenced thus far and integrating the first fossil evidence of Pecluma. Using herbarium material, we applied a genome-skimming approach to obtain a phylogenetic hypothesis of Polypodioideae; assessed the combination of character states observed in the fossil from Miocene Dominican amber using a previously published phylogeny of Polypodioideae based on four plastid markers as framework; calculated divergence times; and conducted an ancestral area estimation. Within Polypodioideae, Pecluma was recovered as sister to Phlebodium. Three of the newly sequenced species-Polypodium otites, P. pinnatissimum, and P. ursipes-were recovered with maximum support within the Pecluma clade, whereas P. christensenii remained within Polypodium. The closest combination of character states of the fossil was found within Pecluma. Our biogeographic analyses suggest an Eocene origin of the genus in South America, with several subsequent Oligocene and Miocene colonization events to Mexico-Central America and to the West Indies. Although the circumscription of Pecluma is still challenging, our results elucidate the origin and age of the genus. The newly described fossil, Pecluma hispaniolae sp. nov., supports the hypothesis that the epiphytic communities of the Greater Antilles exhibit a constant generic composition since the Miocene. We propose new combinations (Pecluma otites, Pecluma pinnatissima, and Pecluma ursipes) to accommodate three species previously classified in Polypodium.

A new species of Nabidae (Hemiptera, Heteroptera, Cimicomorpha) from Dominican amber

ABSTRACT A new species Arachnocoris furtivinabis Ma, Du et Yao sp. nov. is described and illustrated, which represents the first fossil record of Arachnocoris, also the first record in the family Nabidae from Cenozoic Dominican amber. Based on the distinctive morphological characters in this new species, including antennomere II much shorter than twice of antennomere I, labial segment III slightly longer than segment II, and parameres round, it is differentiated from other species within Arachnocoris. Compared with the spider-webdwelling taxa of the extant and extinct species in Heteroptera, the evolution of predation strategies for this special group during the Miocene was discussed.

First record of jumping plant lice of the family Liviidae (Hemiptera, Sternorrhyncha, Psylloidea) from Dominican amber

First record of jumping plant lice of the family Liviidae (Hemiptera, Sternorrhyncha, Psylloidea) from Dominican amber

The first fossil genus and two new species of the worldwide tribe Prosopochrysini (Diptera: Stratiomyidae: Stratiomyinae) found in Dominican and Mexican ambers

ABSTRACT Stratiomyidae is one of the most species-rich and morphologically diversified Diptera families of the orthorraphous Brachycera. Despite the large species diversity and prevalence in various environments, soldier flies are rare in the fossil records, especially in amber deposits. Here we present the first two Prosopochrysini fossils from Miocene amber. The fossils are assigned to a new genus—Cenorhaphiocerina gen. nov.— included in the extant tribe Prosopochrysini, with the description of two species, C. dominicana sp. nov. from amber mines in the Dominican Republic and C. kraemerae sp. nov. from amber areas in Simojovel, Chiapas, Mexico. The new genus and species are compared with other extant genera of Prosopochrysini. We postulate that Cenorhaphiocerina gen. nov. likely forms a group with the extant genera Acanthasargus White, 1914, Hoplistopsis James, 1950, Rhaphiocerina Lindner, 1936 and the species Prosopochrysa azurea (Lindner, 1951), that should be placed in a separate genus, based on the presence of four medial wing veins, upper and lower frons in the head contrasting in colour, and of some level of dichoptic eyes configuration in males. https://zoobank.org:act:1163F677-D54F-4CB8-A11B-9072E76EE91F https://zoobank.org:act:ADDDBA20-883B-41FB-9C28-3F11E3C34308 https://zoobank.org:act:567CEBF3-7E6D-4128-981D-3CC1DB25F0D5

Thought to Be Extinct, but Still Alive Today: The Miocene Genus Primascena Klimaszewsi, 1997 (Hemiptera: Psyllidae) in the Light of Two Extant Species from Brazil.

Fossils can document the morphological diversification through time and date lineages, providing relevant characters are preserved. Primascena Klimaszewsi, 1997 was erected for P. subita Klimaszewsi, 1997 on the basis of a single, partly damaged male from Dominican amber. Originally assigned to Rhinocolidae: Paurocephalinae, the genus was subsequently transferred to Psyllidae: Aphalaroidinae. Recently, two undescribed species resembling the fossil species were discovered in Brazil (Mato Grosso do Sul), allowing a detailed morphological study of adults and immatures. Based on the morphological study, a revised diagnosis of the genus is provided, including the previously unknown female and fifth instar immatures. Primascena subita is redescribed and P. empsycha n. spec. and P. ruprechtiae n. spec. are formally described and illustrated. An identification key is provided for the species of Primascena. A cladistic morphological analysis supports the placement of the two new species in Primascena, and of this genus in the Aphalaroidinae. It is sister to all but Aphalaroida, though with little support. The two Brazilian species develop on Ruprechtia spp. (Polygonaceae: Eriogonoideae), an unusual psyllid host. Immatures of P. ruprechtiae are free-living on the lower leaf face and do not induce galls.

Armoured Lepidopteran Caterpillars Preserved in Non-Fossil Resins and What They Tell Us about the Fossil Preservation of Caterpillars.

Resin is a plastic-like product of trees. Older occurrences of such resin are referred to as amber and are considered fossil resin. Younger resins are termed copals. Even younger ones have been dubbed defaunation resins. Non-fossil resins remain in a terminological limbo, often referred to as "sub-fossils". We report two lepidopteran caterpillars preserved in non-fossil resin: one from Madagascar, one from Brazil. Prominent hairs (=setae) and spines (=spine-like setae) of the specimens make it likely that they represent larvae of Erebidae (e.g., tussock moths and others). So far, most known caterpillars preserved in resins are either "naked" or bear protective cases; only few are armoured with spines or hairs. In particular, long-haired caterpillars such as the ones reported here are so far almost absent. Only one specimen with comparable setae has been reported from 15-million-year-old Dominican amber, but no significant details of this specimen are accessible. We briefly also review the record of caterpillars known from the Holocene, recognising that it is very sparse. The new specimens demonstrate that very hairy caterpillars can readily be preserved in resins in fine detail. Furthermore, the specimens increase the known size range of caterpillars preserved in resins, with one measuring more than 12 mm.

Phimophorus chiodii sp. nov., first representative of a cryptic assassin bug subfamily from Dominican amber (Reduviidae: Phimophorinae) as revealed with traditional light microscopy and computed micro-tomographic reconstruction

Phimophorus chiodii sp. nov., first representative of a cryptic assassin bug subfamily from Dominican amber (Reduviidae: Phimophorinae) as revealed with traditional light microscopy and computed micro-tomographic reconstruction

Notes on Chisosa (Araneae, Pholcidae), with the description of a new species from Mexico.

The genus Chisosa Huber, 2000 previously included only three species of small to tiny North American and Caribbean spiders that are rare in collections and poorly studied. Originally placed in the subfamily Ninetinae, Chisosa is currently considered a representative of Arteminae, close to the North American genus Physocyclus Simon, 1893. This placement has been suggested by molecular data, and it affects the interpretation of morphological characters that were originally thought to support the monophyly of Chisosa: they are shared with Physocyclus and thus plesiomorphic for Chisosa. Here we describe a new species from Mexico, C. calapa sp. nov., and restudy in detail the morphology (incl. ultrastructure) of the type species C. diluta (Gertsch & Mulaik, 1940) and of C. caquetio Huber, 2019, based on newly collected material. We document further similarities with Physocyclus but find only weak morphological support for the monophyly of Chisosa (body size reduction and short legs). In addition, we document surprisingly large genetic distances among C. caquetio specimens from Curaao (>14% CO1 K2P distances), possibly indicating species limits. Finally, we propose that the Dominican amber genus Serratochorus Wunderlich, 1988, based on a single male specimen, is also phylogenetically close to Chisosa and should be included in future studies on these enigmatic spiders.

The second treehopper species in Miocene Dominican amber (Hemiptera: Membracidae)

Summary Stylocentrus pouilloni n. sp., the second species from the mid-Miocene Dominican amber, is described. This new occurrence is important as it enriches the incomplete fossil record of Membracidae, a morphologically highly diversified family among Cicadomorpha, and corroborates the hypothesis of an early divergence of Stegaspidinae within Membracidae.

A new species of the genus Cheiloceps Uhler, 1895 (Hemiptera: Fulgoromorpha: Issidae) from the Dominican amber.

A new species of the planthopper genus Cheiloceps Uhler, 1895 (Issidae, Issinae, Thioniini), Cheiloceps magnifrons sp. nov., is described from the Dominican amber. Issidae fossils are briefly discussed.

Impact of increasing morphological information by micro-CT scanning on the phylogenetic placement of Darwin wasps (Hymenoptera, Ichneumonidae) in amber

The correct interpretation of fossils and their reliable taxonomic placements are fundamental for understanding the evolutionary history of biodiversity. Amber inclusions often preserve more morphological information than compression fossils, but are often partially hidden or distorted, which can impede taxonomic identification. Here, we studied four new fossil species of Darwin wasps from Baltic and Dominican amber, using micro computed tomography (micro-CT) scans and 3D reconstructions to accurately interpret and increase the availability of morphological information. We then infer their taxonomic placement in a Bayesian phylogenetic analysis by combining morphological and molecular data of extant and fossil Darwin wasps and evaluate the impact and usefulness of the additional information from micro-CT scanning. The results show that although we gained significant morphological information from micro-CT scanning, especially concerning measurements and hidden dorsal and ventral structures, this did not impact subfamily-level placement for any of the four fossils. However, micro-CT scanning improved the precision of fossil placements at the genus level, which might be key in future dating and diversification analyses. Finally, we describe the four new fossil species as Rhyssa gulliveri sp. nov. in Rhyssinae, Triclistus levii sp. nov. in Metopiinae, Firkantus freddykruegeri gen. et. sp. nov. in Pimplinae and Magnocula sarcophaga gen. et sp. nov. in Phygadeuontinae. The first two species are the first known representatives of the subfamilies Rhyssinae and Metopiinae in amber.

First pyraloid (Insecta, Lepidoptera) caterpillar from Dominican amber

Only three fossils in the Pyraloidea (Insecta, Lepidoptera) have been confirmed to date, two adults and one larva. The first confirmed larva, in the subfamily Pyraustinae (Crambidae), was described from Baltic amber. Recently, another pyraloid larva from Dominican amber has come to our attention. We describe this second confirmed larval fossil as Penestola wichardi Solis, Léger & Neumann, sp. nov., based on larval morphological characters, such as setal patterns and the shape of their sclerotized bases or pinacula, and place it in the subfamily Spilomelinae (Crambidae).

A new species and new combination in the planthopper genus Cheiloceps Uhler (Hemiptera: Fulgoroidea: Issidae) from Miocene Dominican amber

Cheiloceps arachnophila sp. nov. (Hemiptera: Fulgoroidea: Issidae) is described and illustrated based on a well-preserved adult male, representing the second species of Issidae known from Early Miocene (Burdigalian) Dominican amber. The only previously described issid from Dominican amber is transferred from Thionia Stål to Cheiloceps Uhler: Cheiloceps douglundbergi (Stroiński & Szwedo) comb. nov. Comparative notes on Cheiloceps and Thionia are provided.

‘Dawn’ hexapods in Cenozoic ambers (Diplura: Campodeoidea)

Abstract Diplura are an ancient group of basal (apterygote) hexapods that thrive in various cryptic terrestrial habitats. Despite an ancient origin that extends at least to the Devonian period, the dipluran fossil record is exceedingly sparse. Here, we document five very rare fossil specimens of the family Campodeidae in amber from the Miocene of the Dominican Republic and the Eocene of the Baltic region. Microscopic preservation in amber provides unique detail for taxonomic placement of small, delicate, soil- and leaf litter-dwelling organisms like these. New taxa include the following: in Lepidocampinae, Lepidocampa glaesi sp. nov. (in Dominican amber); and in Campodeinae, Litocampa eobaltica sp. nov. (in Baltic amber) and Rostricampa engeli gen. et sp. nov. (in Dominican amber). Rostricampa has an extraordinary rostrum formed by sclerotized extensions of the clypeus and, probably, the labium, unique among diplurans. These new taxa provide rare additional data on the fossil record of the earliest diverging lineages of the hexapods and shed light on their evolution and ecology.

New records of assassin bugs (Hemiptera: Heteroptera: Reduviidae) for the Dominican Republic

The Hispaniolan fauna of Reduviidae is only partially known, as it has never been the subject of a comprehensive taxonomic or faunistic inventory. These species belong to Neotropical genera that have Caribbean, Central and South American distributions. In this contribution new records are given for 14 species of assassin bugs belonging to six subfamilies. Among these, seven species are first records from the Dominican Republic and the island of Hispaniola: Bactrodes spinulosus Stål, 1862 (Bactrodinae), Lophoscutus rideri Kormilev, 1988, Lophoscutus rugosipes (Guérin-Méneville, 1857) both belonging to Phymatinae, Leogorrus pallipes Stål, 1872 (Reduviinae), and the Saicinae species Oncerotrachelus nasutus (Bergroth, 1913), Tagalis drakkar Varela & Melo, 2017 and Tagalis grossi Gil-Santana, 2011. With these new records the reported extant fauna of Hispaniolan Reduviidae reaches a total of 46 documented species, which are classified in 25 genera and 10 subfamilies. Additionally, 13 fossil species have been reported from the Dominican amber.

The first neotropical ground beetle (Coleoptera, Carabidae) from the Eocene of Ukraine: finding the first Old World ant nest beetle related to Eohomopterus in the Rovno amber

ABSTRACTThe first record of the tribe Paussini Latreille is reported based on a specimen from late Eocene Rovno amber. It is the first known close relative of the genus Eohomopterus (subtribe Carabidomemnina) in the Old World. The recent and Neogene distribution of Eohomopterus is Neotropical, with extant representatives in Ecuador, Brazil and the West Indies, and extinct species in Dominican and Mexican amber. The occurrence of the Neotropical Carabidomemnina in Rovno amber and the presence of the Oriental Protopaussini in Dominican amber are of significant interest as evidence of the probable transarctic migrations of their ant host in the early Eocene.

Paraphloeostiba dominicana sp. nov.: The first omaliine rove beetle from Dominican amber (Coleoptera: Staphylinidae: Omaliinae)

Paraphloeostiba dominicana Shavrin & Jenkins Shaw, sp. nov. from mid-Miocene Dominican amber is described and illustrated. It represents the first species of the rove beetle subfamily Omaliinae from this Neotropical fossil deposit and represents a genus that is not known among the extant fauna of the Neotropics. The new species, embedded within a large piece of amber, was scanned using X-ray micro-computed tomography (μCT), to enable the study of its morphology, including the male genitalia (aedeagus). Based on the revealed characters, the new species is compared with extant and extinct species of the genus Paraphloeostiba Steel, 1960 whose wide extant distribution is confined to the (sub)tropical zones of the world except the Neotropical region and whose fossil record is otherwise known from two species preserved in Eocene Baltic amber from Denmark and Russia. The systematic placement of the genus within the tribe Omaliini, its current and historical ecology and biogeography, as well as the probability that it will be found in the recent Neotropical fauna among the unrevised and undescribed species of Omaliinae are discussed.

Revision of Bethylinae from Dominican amber, with description of a new genus (Hymenoptera, Bethylidae)

Fossil species of bethyline wasps from Dominican amber are revised. Based on a cladistic analysis of female morphological data we recognize a new genus and species, †Crassibethylus dominicanusgen. et sp. nov., which came out as sister group of the clade Odontepyris + Prosierola. We also transfer to Bethylidae a fossil taxon originally described in Sclerogibbidae, placing †Pterosclerogibba Olmi as a new junior synonym under Goniozus Förster, in the new combination †Goniozus antiquus (Olmi). Additionally, the third species of Bethylinae known from Dominican amber, †Goniozus respectus Sorg, is diagnosed and illustrated with color images of the holotype. An identification key to the species of Bethylinae from Dominican amber is provided.