Domestic Camels Research Articles

Seroprevalence and associated risk factors of peste des petits ruminants in sheep and goats in three districts of the Central Oromia Region, Ethiopia.

Peste des petits ruminants (PPR) is a viral disease that affects domestic and wild small ruminants and camels in Africa, the Middle East, and Asia. Following the successful eradication of rinderpest, the World Organization for Animal Health (WOAH) and the Food and Agriculture Organization (FAO) have undertaken to eradicate PPR by 2030. Regular surveillance and monitoring of the disease in various regions of Ethiopia are crucial to achieving this goal. This study aimed to estimate the prevalence of PPR, assess community awareness of PPR infection, and examine the associated risk factors of the disease in selected districts of the Central Oromia Region, Ethiopia. The study collected 384 serum samples from 73 flocks containing 217 sheep and 167 goats using a multi-stage sampling technique. A competitive enzyme-linked immunosorbent assay (c-ELISA) was used to detect antibodies against the PPR virus. Additionally, a pre-tested questionnaire was used to gather information on community awareness and potential risk factors for PPRV infection in the study area. The study found that the overall prevalence of PPR in flocks was 71.2% [95% confidence interval (CI): 59.4%-81.2%]. The prevalence of PPR at the animal level was 50% (95% CI: 44.9%-55.1%), with sheep having a prevalence of 54.4% (95% CI: 47.0%-60.6%) and goats having a prevalence of 44.3% (95% CI: 36.6%-52.2%). The study also found that districts, flock size, and agroecology were independent predictors of PPRV seropositivity in sheep, whereas districts, origin, and mixed species were independent predictors of PPRV seropositivity in goats. The study revealed a high prevalence of PPR in sheep and goats in the study area. To prevent the spread of the disease, the study suggests quarantining animals before introducing them to districts, regular PPR vaccination, and isolation and molecular characterization of the PPR virus circulating in the study area.

Identification of novel genetic loci related to dromedary camel (Camelus dromedarius) morphometrics, biomechanics, and behavior by genome-wide association studies

In the realm of animal breeding for sustainability, domestic camels have traditionally been valued for their milk and meat production. However, key aspects such as zoometrics, biomechanics, and behavior have often been overlooked in terms of their genetic foundations. Recognizing this gap, the present study perfomed genome-wide association analyses to identify genetic markers associated with zoometrics-, biomechanics-, and behavior-related traits in dromedary camels (Camelus dromedarius). 16 and 108 genetic markers were significantly associated (q < 0.05) at genome and chromosome-wide levels of significance, respectively, with zoometrics- (width, length, and perimeter/girth), biomechanics- (acceleration, displacement, spatial position, and velocity), and behavior-related traits (general cognition, intelligence, and Intelligence Quotient (IQ)) in dromedaries. In most association loci, the nearest protein-coding genes are linkedto neurodevelopmental and sensory disorders. This suggests that genetic variations related to neural development and sensory perception play crucial roles in shaping a dromedary camel’s physical characteristics and behavior. In summary, this research advances our understanding of the genomic basis of essential traits in dromedary camels. Identifying specific genetic markers associated with zoometrics, biomechanics, and behavior provides valuable insights into camel domestication. Moreover, the links between these traits and genes related to neurodevelopmental and sensory disorders highlight the broader implications of domestication and modern selection on the health and welfare of dromedary camels. This knowledge could guide future breeding strategies, fostering a more holistic approach to camel husbandry and ensuring the sustainability of these animals in diverse agricultural contexts.

Genomic signatures of positive selection in Awarik dromedary camels from southwestern of Saudi Arabia.

The Awarik camel population in southwestern Saudi Arabia exhibits unique genetic and phenotypic traits compared to other domestic camel populations. This study aims to explore the genomic signatures of positive selection in Awarik camels to understand their evolutionary history and identify genetic adaptations potentially shared with East African camel populations. Whole genome sequencing data from nine Awarik camels were analyzed using two robust intra-population haplotype-based statistical methods: integrated haplotype score (iHS) and number of segregating sites by length (nSL). These analyses were conducted to identify candidate regions under positive selection within the Awarik camel genome. These analyses identified 66 and 53 candidate selection regions, encompassing 185 and 123 genes, respectively. The iHS analysis revealed significant selection signals on chromosomes 15 and 16, including a robust overlap on chromosome 15 (10 regions) involving the TRNAI-AAU gene, suggesting its critical role in adaptive processes. Additionally, chromosome 3 exhibited the highest number of candidate regions totaling 10. The nSL analysis highlighted statistically significant regions on chromosomes 2 and 7, as well as a high concentration of candidate regions on chromosome 14, totaling five regions. Notably, large candidate regions were also identified on chromosome 11 (200 kb: 51.750-51.950 kb) and chromosome 9 (325 kb: 45.825-46.150 kb). Functional annotation of these genes revealed involvement in diverse biological processes including olfactory activity, immune regulation, metabolism, insulin secretion, reproductive performance, kidney function, and cellular signaling, with specific genes like BAG5, septin 7, SLC13A1, PCED1B, BMPR1B, ZAR1, JAKMIP2, and NOTCH2 highlighted. These findings contribute to our understanding of the adaptive mechanisms of Awarik camels and have important implications for breeding and conservation strategies. Further research on these genetic adaptations, particularly those affecting immune response, is crucial to mitigate the impacts of climate change on camel populations.

Camelus knoblochi genome reveals the complex evolutionary history of Old World camels

Extant Old World camels (genus Camelus) contributed to the economic and cultural exchanges between the East and West for thousands of years.1,2 Although many remains have been unearthed,3,4,5 we know neither whether the prevalent hybridization observed between extant Camelus species2,6,7 also occurred between extinct lineages and the ancestors of extant Camelus species nor why some populations became extinct while others survived. To investigate these questions, we generated paleogenomic and stable isotope data from an extinct two-humped camel species, Camelus knoblochi. We find that in the mitochondrial phylogeny, all C.knoblochi form a paraphyletic group that nests within the diversity of modern, wild two-humped camels (Camelus ferus). In contrast, they are clearly distinguished from both wild and domesticated (Camelus bactrianus) two-humped camels on the nuclear level. Moreover, the divergence pattern of the three camel species approximates a trifurcation, because the most common topology is only slightly more frequent than the two other possible topologies. This mito-nuclear phylogenetic discordance likely arose due to interspecific gene flow between all three species, suggesting that interspecific hybridization is not exclusive to modern camels but a recurrent phenomenon throughout the evolutionary history of the genus Camelus. These results suggest that the genomic complexity of Old World camels' evolutionary history is underestimated when considering data from only modern species. Finally, we find that C.knoblochi populations began declining prior to the last glacial maximum and, by integrating palaeoecological evidence and stable isotope data, suggest that this was likely due to failure to adapt to a changing environment.

SARS-CoV-2 seroprevalence determination in pets and camels in Egypt using multispecies enzyme-linked immunosorbent assay

The coronavirus disease 2019 (COVID-19) pandemic has translated into a worldwide economic recession and public health crisis. Bats have been incriminated as the main natural host for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the causative agent of the COVID-19 pandemic. However, the reservoir and carrier hosts of the virus remain unknown. Therefore, a cross sectional serosurvey study was performed to estimate antibodies to SARS-CoV-2. To assess IgM antibodies to SARS-CoV-2 nucleocapsid protein (NP), a SARS-CoV-2 Double Antigen Multispecies diagnostic enzyme-linked immunosorbent assay kit was used. The seropositive samples were confirmed and validated by measuring IgG antibody titers in sera. The enrolled animals were from different locations in the Giza governorate, Egypt, and were sampled at the time of the pandemic; they comprised 92 companion animals and 92 domestic camels. The study established that 4.76% (1/21 clinical samples) of dogs, 7.69% of cats (1/13 shelter samples) and 1.08% (1/92) of camels, had measurable SARS-CoV-2 NP IgM antibodies. All IgM-seropositive samples were IgG positive with a measurable titer of 34.5, 28.6, and 25.8 UI/mL for dog, cat, and camels, respectively. According to our best knowledge, this study was the first to assess SARS-CoV-2 seroprevalence in the specific animals investigated in Egypt. These results may herald a promising epidemiological role for pet animals and camels in SARS-CoV-2 virus maintenance. Thus, our study's results ought to be confirmed with a nationwide seroprevalence study, and further studies are required to clarify whether these animals act as active or passive carriers.

Question of agent of camel balantidiosis solved: Molecular identity, taxonomic solution and epidemiological considerations

Domestic camels (Camelus bactrianus, the Bactrian camel; and Camelus dromedarius, the dromedary) are pseudo-ruminant herbivores kept as livestock in rural, inhospitable regions (cold deserts and dry steppes of Asia, arid to semi-arid regions of Africa, western and central Asia). Their close contact with humans makes them a potential reservoir for zoonotic parasite infections, as has been suggested for human balantidiasis. However, there is confusion about the ciliate species that infects camels: Infundibulorium cameli was originally described in dromedaries, but this name has almost never been used and most authors identified their findings as Balantioides coli and, to a lesser extent, Buxtonella sulcata, a cattle ciliate. To clarify the taxonomic status of the parasite and the corresponding zoonotic significance for camels, we performed morphological characterization of cysts and genetic analysis (SSU-rDNA and ITS markers) of B. coli-like isolates from Bactrian camels from Bulgaria and from dromedaries from Spain and the United Arab Emirates. Our results indicate that the camel ciliate is not B. coli, nor is it B. sulcata, but is a different species that should be placed in the same genus as the latter. Thus, camels are not a reservoir for human balantidiasis. Although the correct genus name would be Infundibulorium according to the principle of priority, this would lead to confusion since this name has almost fallen into disuse since its initial description, but Buxtonella is almost universally used by researchers and veterinarians for the cattle ciliate. We therefore propose to apply the reversal of precedence and use Buxtonella as the valid genus name. Consequently, we propose Buxtonella cameli n.comb. as the name for the camel ciliate.

Comprehensive investigation of milk oligosaccharides in different mammalian species and the effect of breed and lactation period on sheep milk oligosaccharides

Milk oligosaccharides (MOs) have unique health benefits for newborns, and MOs are important components in mammalian milk. The present study was conducted to provide a comprehensive analysis of MOs in important domestic animals, including goats, cows, camels and sheep. The comparison with human MOs was conducted simultaneously. Furthermore, analysis of the relative abundance of sheep MOs among different breeds (Hu sheep, East Friesen sheep, East Friesen-Hu crossbred sheep) and lactation periods (colostrum, mature milk) was performed. In general, 35, 24 19, 26, and 16 MOs were identified in human, goat, bovine, camel and sheep milk, respectively. The type of sheep MOs was not greatly influenced by the breeds and lactation period. Hu sheep colostrum had the highest abundance of MOs among six sheep milks, followed by East Friesen sheep colostrum, while East Friesen-Hu crossbred sheep mature milk had the lowest abundance of MOs. These findings provide evidence for the potential value of MOs from domestic animal milk for the commercial applications.

Lungworms of Non-Ruminant Terrestrial Mammals and Humans in Iran.

With over 300 terrestrial and aquatic mammalian species, Iran is considered a country with an ample mastofauna. Although many studies have assessed the distribution of gastrointestinal helminth parasites in animals and humans in Iran, lungworms have not received adequate attention. Following a previous article in which we reviewed the diversity and prevalence of lungworm infections in pastoral and wild ruminants of Iran, this report compiles the available scientific information about the occurrence of lungworms in non-ruminant mammals and humans from 1980 to 2022 to provide insights into the epidemiology of these infections. International and national scientific databases were searched, and twenty-six articles in peer-reviewed journals, one conference paper, and one D.V.M. thesis were included in the study. In total, 10 species belonging to seven genera, including Dictyocaulus, Deraiophoronema, Protostrongylus, Crenosoma, Eucoleus, Aelurostrongylus, and Metastrongylus, were reported in the respiratory tract or feces of humans, domestic animals (i.e., camels, equids, dogs, and cats), and wildlife species (i.e., hedgehogs, wild boars, and hares). Most of the studies (22/28) were performed using post-mortem examinations. The overall prevalence of respiratory nematode infection varied according to animal species in camels (14.83%), equids (13.31%), dogs (5%), wild boars (45.66%), hedgehogs (42.57%), and hares (1.6%). In addition, pulmonary capillariasis caused by Eucoleus aerophilus was reported in a 9 year old child. The prevalence of lungworm species in domestic camels, equids, and dogs, combined with a lack of labeled anthelmintic products, supports the need to improve our understanding of these important nematode parasites and inform the development of sustainable control strategies. From a zoo and wildlife medicine point of view, there is a shortage of information about the presence and prevalence of lungworm infections in the majority of mammalian species, pending epidemiological studies that integrate classical parasitology and molecular methods.

Lunar Cycle, Climate, and Onset of Parturition in Domestic Dromedary Camels: Implications of Species-Specific Metabolic Economy and Social Ecology.

Given energy costs for gestating and caring for male offspring are higher than those of female newborns, external environmental conditions might be regarded as likely to affect the timing of delivery processes differentially depending on the sex of the newborn calf to be delivered. The aim of the present paper is to evaluate the association between environmental stressors such as the moon phase and weather-related factors and the onset of labor in female dromedaries. A binary logistic regression model was developed to find the most parsimonious set of variables that are most effective in predicting the probability for a gravid female dromedary to give birth to a male or a female calf, assuming that higher gestational costs and longer labor times are ascribed to the production of a male offspring. Although the differences in the quantitative distribution of spontaneous onset of labor across lunar phases and the mean climate per onset event along the whole study period were deemed nonsignificant (p > 0.05), a non-negligible prediction effect of a new moon, mean wind speed and maximum wind gust was present. At slightly brighter nights and lower mean wind speeds, a calf is more likely to be male. This microevolutionary response to the external environment may have been driven by physiological and behavioral adaptation of metabolic economy and social ecology to give birth to cooperative groups with the best possible reduction of thermoregulatory demands. Model performance indexes then highlighted the heterothermic character of camels to greatly minimize the impact of the external environment. The overall results will also enrich the general knowledge of the interplay between homeostasis and arid and semi-arid environments.

A Brief Review of Endemic Fluorosis in Dromedary Camels (Camelus Dromedarius) and Focus on Their FluorideSusceptibility

Chronic fluoride poisoning in the form of fluorosis in diverse species of domestic animals, viz. cattle (Bos taurus), water buffaloes (Bubalus bubalis), sheep (Ovis aries), goats (Capra hircus), horses (Equus caballus), donkeys (Equus asinus), and dromedary camels (Camelus dromedarius) is the resultant of prolonged consumption or exposure of high fluoride through drinking water, food, and air. In general, fluorosis can be recognised by the presence of dental mottling (dental fluorosis) and bone deformities (skeletal fluorosis). In animals, skeletal fluorosis often results in reduced mobility as a result of bone damages or changes due to exostosis, osteosclerosis, osteoporosis, and osteophytosis. In addition, fluoride-induced non-skeletal disorders like gastro-intestinal disturbances, neurological disorders, reproductive dysfunctions, apoptosis, excitotoxicity, genotoxicosis, and teratogenic effects have also been observed in fluorosed domestic and experimental animals. The maximum prevalence, 19.4% and 17.9% of dental and skeletal fluorosis at &gt;3.0 ppm fluoride concentration in drinking water has been observed in domesticated dromedary camels in India, respectively. The prevalence and severity of fluorosis varies from one fluoride endemic region to another and depends on a number of determinants or factors. However, the major determinants are fluoride concentration and its frequency and duration of exposure, rate and density of bio-accumulation of fluoride, and food nutrients. Among different species of domestic animals, camels are found to be less susceptible to fluoride or have a relatively higher fluoride tolerance. In present communication, fluorosis in camels, determinants of fluoride toxicity, and fluoride susceptibility in camels are briefly and critically reviewed. Simultaneously, research gapes have also been highlighted for further research work on chronic fluoride intoxication in diverse species of animals. This review is significant for researchers in understanding of chronic fluoride toxicity in grazers (cattle and buffalo) and browsers (equines, flocks, and camels).

Elemental characterization of freeze dried domestic animals’ milk using ion beam analysis techniques

Particle induced X-ray emission (PIXE) and particle induced γ-ray emission (PIGE) techniques were employed to perform elemental analysis in four milk samples of local domestic animals, namely sheep, caw, camel and goat. Additionally, Rutherford backscattering (RBS) spectra were acquired simultaneously with PIXE and PIGE to determine matrix elements. Milk samples were prepared in a simple dried base following freeze drying process. Optimization of PIXE/PIGE/RBS experimental conditions as well as data acquisition and analysis procedures were carefully addressed. Verification study was performed relying on the analysis of both IAEA-11 and IAEA-153 reference materials. Accuracies of ±5–10% together with relative standard deviations of less than 15% were mostly reported. Chemical element concentrations of 11 elements were determined, namely Na, Mg, P, S, Cl, K, Ca, Zn, Br, Rb and Sr. Obtained element concentration values were mostly in the range 1000–10000 μg/g for major elements and 5–100 μg/g for trace elements. No significant changes in elemental concentration differences for both trace and major elements were noticed in the four different milk samples. The contribution of the elements in these milk samples to the dietary recommended intakes (DRI) has been evaluated. Advantages of applying multiple ion beam analysis techniques have been discussed.

Asian Journal of Pharmaceutical Research

Camel urine has been widely used in the biomedical field as a traditional healing liquid for several health disorders, this study aims to evaluate the antioxidant activity of camel urine and its association with the breeding and the feeding system. Urine samples were collected from domestic (from private farms) and desertic camels, where spectrophotometric method was chosen to evaluate the phenolic, flavonoid content and the antioxidant activity. As results, it was found from the applied testes that the antioxidant activity of the camel urine is very important, where both types of urine illustrated a very low EC50. However, it has been found that the significant anti-radical activity and a reducing power of urine of domesticated camels fed in private farms was higher than the urine of desertic camels. concluding that the consumption and usage of camel urine can contribute to the prevention of diseases associated with oxidative stress.

Microsatellite markers of the major histocompatibility complex genomic region of domestic camels.

We identified and characterized 11 polymorphic microsatellite markers suitable for routine testing (three in the MHC class I sub-region, four in MHC class II and four in the MHC class III sub-region) of dromedaries and Bactrian camels. In total, 38 dromedaries and 33 Bactrian camels were genotyped, and interspecific differences were observed in the numbers of alleles and in allelic frequencies, as well as in the observed heterozygosity. These loci may be used as markers to study the adaptive genetic diversity of the MHC region in Old World camels.

Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels

IntroductionPeste des petits ruminants virus (PPRV) causes a highly devastating disease of sheep and goats, peste des petits ruminants (PPR), which is targeted for global control and eradication by 2030. The serological diagnostic tool kits for accurate diagnosis of PPR have inherent strengths and weaknesses that require parallel validation and optimization across animal species. Thus, the objective of this study was to evaluate diagnostic performance of haemagglutinin based PPR blocking ELISA (HPPR- b-ELISA), that was developed by Africa Union Pan African Veterinary Vaccine Center for specific detection of anti- PPRV antibodies.MethodsIn preliminarily investigation, diagnostic performance of the HPPR-b-ELISA®, commercial PPR competition ELISA (c-ELISA) and virus neutralization test (VNT) were compared for the detection of anti-PPRV antibodies in goats, sheep, cattle and camels.ResultsThe sensitivity and specificity of HPPR- b-ELISA® were 79.55 and 99.74%, respectively, compared to c-ELISA. The HPPR- b-ELISA® was in perfect agreement (κ = 0.86) with the c-ELISA in all sera collected from goats, sheep and cattle. There was almost perfect agreement between the species of goats (κ = 0.82) and sheep (κ = 0.98), while the agreement was substantial in cattle (κ = 0.78) and no agreement was observed in camels (κ = 0.00). Similarly, the sensitivity and specificity of the HPPR b-ELISA were 80 and 96.36%, respectively compared to VNT with almost perfect agreement in goats (κ = 0.83) and sheep (κ = 0.89), moderate in cattle (κ = 0.50) and none in camels (κ = 0.00).ConclusionOur study revealed that HPPR- b-ELISA is a suitable and valid method that can alternatively be used for screening and monitoring of PPR in sheep, goats and cattle except for camels.

Refining the Camelus dromedarius Myostatin Gene Polymorphism through Worldwide Whole-Genome Sequencing.

Simple SummaryThe dromedary is a multipurpose livestock species. Growing interest in dromedary meat production, increasing intensification of meat production systems and rising business turnover for the dromedary athletic competition sector point to the need for a more effective genetic selection. To this aim, the identification of genetic markers, to be implemented in phenotype–genotype association studies, represents a pre-requisite. The myostatin gene is known to affect, to a various extent, muscularity in several animal species. In this study, we investigated the dromedary myostatin gene sequence variation through the analysis of data from 183 worldwide animals. A total of 99 variants were detected in the target region. Through a bioinformatic approach, we explored the possible functional effects of the detected variants. Several hints emerged, suggesting that natural variants at the dromedary myostatin locus may determine quali-quantitative changes in the myostatin expression, thus likely impacting on dromedary muscularity. Further efforts are needed to collect reliable phenotypic data on dromedary muscularity and racing performances, thus allowing for the unveiling of those genetic markers that are associated with the phenotypic variability in muscularity-related traits. Under intensive rearing systems, large-scale screening of genotypes at those markers in dromedary populations will effectively orient selection decisions and positively impact on rates of genetic gain.Myostatin (MSTN) is a highly conserved negative regulator of skeletal muscle in mammals. Inactivating mutations results in a hyper-muscularity phenotype known as “double muscling” in several livestock and model species. In Camelus dromedarius, the gene structure organization and the sequence polymorphisms have been previously investigated, using Sanger and Next-Generation Sequencing technologies on a limited number of animals. Here, we carried out a follow-up study with the aim to further expand our knowledge about the sequence polymorphisms at the myostatin locus, through the whole-genome sequencing data of 183 samples representative of the geographical distribution range for this species. We focused our polymorphism analysis on the ±5 kb upstream and downstream region of the MSTN gene. A total of 99 variants (77 Single Nucleotide Polymorphisms and 22 indels) were observed. These were mainly located in intergenic and intronic regions, with only six synonymous Single Nucleotide Polymorphisms in exons. A sequence comparative analysis among the three species within the Camelus genus confirmed the expected higher genetic distance of C. dromedarius from the wild and domestic two-humped camels compared to the genetic distance between C. bactrianus and C. ferus. In silico functional prediction highlighted: (i) 213 differential putative transcription factor-binding sites, out of which 41 relative to transcription factors, with known literature evidence supporting their involvement in muscle metabolism and/or muscle development; and (ii) a number of variants potentially disrupting the canonical MSTN splicing elements, out of which two are discussed here for their potential ability to generate a prematurely truncated (inactive) form of the protein. The distribution of the considered variants in the studied cohort is discussed in light of the peculiar evolutionary history of this species and the hypothesis that extremely high muscularity, associated with a homozygous condition for mutated (inactivating) alleles at the myostatin locus, may represent, in arid desert conditions, a clear metabolic disadvantage, emphasizing the thermoregulatory and water availability challenges typical of these habitats.

Genetic diversity of hepatitis E virus (HEV) in imported and domestic camels in Saudi Arabia

Camels gained attention since the discovery of MERS-CoV as intermediary hosts for potentially epidemic zoonotic viruses. DcHEV is a novel zoonotic pathogen associated with camel contact. This study aimed to genetically characterize DcHEV in domestic and imported camels in Saudi Arabia. DcHEV was detected by RT-PCR in serum samples, PCR-positive samples were subjected to sequencing and phylogenetic analyses. DcHEV was detected in 1.77% of samples with higher positivity in domestic DCs. All positive imported dromedaries were from Sudan with age declining prevalence. Domestic DcHEV sequences clustered with sequences from Kenya, Somalia, and UAE while imported sequences clustered with one DcHEV isolate from UAE and both sequences clustered away from isolates reported from Pakistan. Full-genome sequences showed 24 amino acid difference with reference sequences. Our results confirm the detection of DcHEV in domestic and imported DCs. Further investigations are needed in human and camel populations to identify DcHEV potential zoonosis threat.

Genetic Diversity of the Mongolian Bactrian Camel Based on Mitochondrial Sequences

Current genetic diversity of 5 local breeds of Mongolian camels and 2 samples of Camelus ferus was studied and compared their levels of variability. DNA was extracted using Qiaamp® DNA kit (Qiagen) and amplified mtDNA D-loop control region from 36 blood samples of Mongolian Bactrian camels and 2 from Mongolian Camelus ferus. Three common haplotypes (H1, H2, H6) and 4 unique haplotypes (H3, H4, H5, H8) revealed in the population of domestic camels based on the mitochondrial DNA region. For the further study we carried out best result 26 sequences. We did not find high inbreeding levels in the different breeds. The phylogenetic analysis suggested that domestic and wild camels have two distinct lineages.

Investigation of SNPs in genes related to lipid and glucose metabolism in the genome of dromedary, domestic Bactrian and wild Bactrian camels

The aim of the present study was to identify the SNPs located in the genes that are associated with lipid and glucose metabolism in the genome of Dromedary, domestic and wild Bactrian camels. For this purpose, firstly, important genes that related to lipid (15 genes) and glucose (17 genes) metabolism in mammalian were identified using DDD analysis and also reviewing of the authentic papers. In order to evaluate the accuracy of selected genes, gene ontology analysis was performed on them and significant lipid- and glucose-related terms were observed. In the next step, common SNPs among three Dromedaries (993,474), four domestic Bactrian (1,246,741) and two wild Bactrian camels (2,586,654) were extracted, identification and classification were done for SNPs of studied genes in the whole genome of three camel species. The results showed that the most of the identified SNPs are located in the intron, upstream and downstream of the genes that considered as regulatory variants. Some genes showed different pattern of SNPs among three specises, so that, several genes had SNPs in some species and no variant in other species. Identified genes, especially those that showed different variant pattern in camels, may be considered as options for studying of evolution presses of these animals.

Review of Peste des Petits Ruminants Occurrence and Spread in Tanzania.

Simple SummaryPeste des petits ruminants (PPR), caused by PPR virus (PPRV), is a transboundary animal disease of sheep and goats that has a significant impact on farmer’s livelihoods, food and nutritional security; and threatens susceptible wildlife. This review compiled information on the introduction and spread of PPR in Tanzania, from published and unpublished sources. PPR was first confirmed in Tanzania in 2008, but could have been present earlier, based on antibody detection in archived sera. The virus was probably introduced to northern Tanzania through cross-border movement of sheep and goats, and afterwards spread to eastern, central and southern Tanzania through movement of animals by pastoralists and traders. Genome sequencing shows that there have been several introductions of PPRV and it is now considered to be endemic. PPR has not been observed in cattle, camels or wildlife, but sera collected from these species contain PPRV antibodies, indicating virus exposure, probably through contact with infected sheep and goats. Some challenges for PPR control in Tanzania include the spread of the disease through small ruminants movements for pastoralism and trade, and limited veterinary services for disease surveillance and vaccination. The socio-economic impact of PPR justifies investment in a comprehensive disease eradication programme.Peste des petits ruminants (PPR) is an important transboundary animal disease of domestic small ruminants, camels, and wild artiodactyls. The disease has significant socio-economic impact on communities that depend on livestock for their livelihood and is a threat to endangered susceptible wild species. The aim of this review was to describe the introduction of PPR to Tanzania and its subsequent spread to different parts of the country. On-line databases were searched for peer-reviewed and grey literature, formal and informal reports were obtained from Tanzanian Zonal Veterinary Investigation Centres and Laboratories, and Veterinary Officers involved with PPR surveillance were contacted. PPR virus (PPRV) was confirmed in northern Tanzania in 2008, although serological data from samples collected in the region in 1998 and 2004, and evidence that the virus was already circulating in Uganda in 2003, suggests that PPRV might have been present earlier than this. It is likely that the virus which became established in Tanzania was introduced from Kenya between 2006–7 through the cross-border movement of small ruminants for trade or grazing resources, and then spread to eastern, central, and southern Tanzania from 2008 to 2010 through movement of small ruminants by pastoralists and traders. There was no evidence of PPRV sero-conversion in wildlife based on sera collected up to 2012, suggesting that they did not play a vectoring or bridging role in the establishment of PPRV in Tanzania. PPRV lineages II, III and IV have been detected, indicating that there have been several virus introductions. PPRV is now considered to be endemic in sheep and goats in Tanzania, but there has been no evidence of PPR clinical disease in wildlife species in Tanzania, although serum samples collected in 2014 from several wild ruminant species were PPRV sero-positive. Similarly, no PPR disease has been observed in cattle and camels. In these atypical hosts, serological evidence indicates exposure to PPRV infection, most likely through spillover from infected sheep and goats. Some of the challenges for PPRV eradication in Tanzania include movements of small ruminants, including transboundary movements, and the capacity of veterinary services for disease surveillance and vaccination. Using wildlife and atypical domestic hosts for PPR surveillance is a useful indicator of endemism and the ongoing circulation of PPRV in livestock, especially during the implementation of vaccination to control or eliminate the disease in sheep and goats. PPR disease has a major socio-economic impact in Tanzania, which justifies the investment in a comprehensive PPRV eradication programme.

Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan.

Middle East respiratory syndrome-related coronavirus (MERS-CoV) is a persistent zoonotic pathogen with frequent spillover from dromedary camels to humans in the Arabian Peninsula, resulting in limited outbreaks of MERS with a high case-fatality rate. Full genome sequence data from camel-derived MERS-CoV variants show diverse lineages circulating in domestic camels with frequent recombination. More than 90% of the available full MERS-CoV genome sequences derived from camels are from just two countries, the Kingdom of Saudi Arabia (KSA) and United Arab Emirates (UAE). In this study, we employ a novel method to amplify and sequence the partial MERS-CoV genome with high sensitivity from nasal swabs of infected camels. We recovered more than 99% of the MERS-CoV genome from field-collected samples with greater than 500 TCID50 equivalent per nasal swab from camel herds sampled in Jordan in May 2016. Our subsequent analyses of 14 camel-derived MERS-CoV genomes show a striking lack of genetic diversity circulating in Jordan camels relative to MERS-CoV genome sequences derived from large camel markets in KSA and UAE. The low genetic diversity detected in Jordan camels during our study is consistent with a lack of endemic circulation in these camel herds and reflective of data from MERS outbreaks in humans dominated by nosocomial transmission following a single introduction as reported during the 2015 MERS outbreak in South Korea. Our data suggest transmission of MERS-CoV among two camel herds in Jordan in 2016 following a single introduction event.