DM Losses Research Articles

The Quality characteristics of haylage from forage grasses of tropical pastures: losses, gas production, nutritional value, microbial population and organic acids

Tropical pastures have been little explored for haylage, due to the lack of information on nutritional quality. This study aimed to evaluate the fermentation quality of different genotypes of forage grasses from tropical pastures in the form of haylage. Six genotypes of grasses were used to evaluate the fermentation characteristics, losses and presence of microorganisms in the in the haylage. The completely randomized design was used for all variables. Data were subjected to analysis of variance with all genotype’s means compared by the Scott Knott’s test at 5% probability. There was effect of interaction between form of material x genotype (P<0.01) on all variables of chemical composition evaluated in the in natura and haylage at the moment of haylage making, except for NDF and N-NH3 (P>0.05). The concentrations of O2 in the haylage after 60 days of storage were lower for the genotypes Xaraés and Tupã. The highest levels of acetic acid were observed in genotypes Xaraés, Planaltina and Tupã. The Planaltina genotype presented the highest DM loss in the process of production and storage of the haylage, averaging 8.2 ± 0.37%. The haylage of the species B. brizantha and M. maximus presented better nutritional characteristics.

Effects of bacteriocin-producing Lactiplantibacillus plantarum on bacterial community and fermentation profile of whole-plant corn silage and its in vitro ruminal fermentation, microbiota, and CH4 emissions

BackgroundSilage is widely used to formulate dairy cattle rations, and the utilization of antibiotics and methane emissions are 2 major problems for a sustainable and environmentally beneficial ruminant production systems. Bacteriocin has received considerable attention because of its potential as an alternative to antibiotics in animal husbandry. However, the impact of bacteriocin-producing lactic acid bacteria on the microbiological conversion process of whole-plant corn silage and rumen fermentation remains limited. The purpose of this study was to assess the effect of 2 class IIa bacteriocin-producing strains Lactiplantibacillus plantarum ATCC14917 and CICC24194 on bacterial community composition and ensiling profiles of whole-plant corn silage and its in vitro rumen fermentation, microbiota, and CH4 emissions.ResultsBoth bacteriocin-producing strains increased the lactic acid concentration in silage fermented for 7 d, whereas the lowest lactic acid was observed in the ATCC14917 inoculated silage fermented for 90 d (P < 0.05). The highest DM content was observed in the CICC24194 treatment (P < 0.05), and the silages treated with both strains had the lowest DM loss (P < 0.05). Bacteriocin-producing strains promoted the growth of Levilactobacillus brevis on d 60 of ensiling. In addition, treatment with bacteriocin-producing strains increased the in vitro DM digestibility (P < 0.05) and decreased the CH4 production (P < 0.05). The results of random forest and clustering analyses at the genus level showed that ATCC14917 increased the relative abundance of the influential variable Bacillus compared to that in the control group, whereas CICC24194 decreased the relative abundance of the influential variable Ruminococcaceae UCG-005. The CICC24194 treatment had the lowest total bacterial, fungal, protozoan, and methanogen populations (P < 0.05).ConclusionsBoth class IIa bacteriocin-producing L. plantarum strains improved the fermentation quality of whole-plant corn silage by regulating the bacterial community composition during ensiling, with CICC24194 being the most effective. Both bacteriocin-producing strains mitigated CH4 production and improved digestibility by modulating the interactions among rumen bacteria, protozoa, methanogens, and the composition of fibrolytic bacteria.

Effect of distillery wastewater on chemical composition and microbial community of Sorghum propinquum silage during micro-permeation of air

The aim of this study was to investigate the effect of distillery wastewater (DWW) on the nutrient composition, fermentation quality and microbial community of Sorghum propinquum silage during the micro-permeation of air. S. propinquum without (CK) or with L. buchneri, (LAB), distillery wastewater yellow serofluid (Y) and distillery spent wash (S) was ensiled for 60 days, and then subjected to a micro-permeation stability of air test for 6 days. After 60 days of storage, treatments with DWW and LAB decreased the loss of DM, inhibited the degradation of protein and reduced the production of ammonia nitrogen in silage relative to the control. In particular, S. propinquum silage treated with yellow serofluid sustained higher levels. Moreover, the addition of DWW resulted in higher levels of acetic and propionic acid than the other treatments. During the micro-permeation of air, the addition of DWW was effective in inhibiting the reduction of lactic acid content, and unique genera Roseburia and Faecalibacterium, which are beneficial for livestock production, discovered in DWW-treated S. propinquum silage. In conclusion, the addition of DWW was efficacious in improving the nutritional composition and microbial community of S. propinquum silage during the micro-permeation of air.

Effect of Chemical and Microbial Additives on Fermentation Profile, Chemical Composition, and Microbial Populations of Whole-Plant Soybean Silage

This study evaluated the effects of two chemical additives or a microbial inoculant on chemical composition and DM losses in whole-plant soybean silage. One-hundred and twenty mini-silos were used in a completely randomized design experiment with the following treatments: water without chloride (control, CON); a microbial inoculant (INO); a chemical additive containing 35–45% formic acid (FA type); and another chemical additive containing 50–60% propionic acid (PA type). Data were analyzed using mixed models of SAS, and treatment differences were evaluated by the following orthogonal contrasts: C1 = CON vs. additives (INO + FA type + PA type); C2 = INO vs. chemical additives (FA type + PA type); and C3 = PA type vs. FA type. Silage pH and ammonia nitrogen concentration were decreased, and concentrations of lactic acid and acetic acid were increased with additives. Counts of lactic acid bacteria were higher in silages with INO than with chemical additives. DM recovery increased with FA type and PA type. Additives increased DM and CP concentrations. Silage A-fraction proportion was greater with additives. Additives, particularly FA type and PA type, improved chemical composition and fermentative profile and reduced undigestible proportions of protein in whole-plant soybean silage. Chemical additives were more effective in reducing silage DM losses than INO.

Lactic acid bacteria and manganese‐based additives for improved lucerne, perennial grass, and perennial grass‐white clover preservation as silage

AbstractLactic acid bacteria (LAB) are commonly used as silage inoculants. Manganese sulphate (MnSO4) has been proposed to protect certain LAB during oxygen exposure and improve silage aerobic stability (ASTA). The aim of the present study was to assess the efficacy of inoculants in preservation of different forages. A short‐term screening trial investigated the effect of 24 additive treatments, compared to a negative control, on ensiling perennial grass in mini‐silos (2.75 L) for 7 d. The additive treatments comprised different ratios of Lactiplantibacillus plantarum [IMI 507026, IMI 507027, and IMI 507028] and Pediococcus pentacaceus [IMI 507024 and IMI 507025], with or without MnSO4. Target parameters, such as low levels of fresh matter loss, pH, acetic acid, ammonia‐nitrogen fraction (NH3–N/Total N), and high levels of lactic acid and ASTA, were considered for treatment selection. Eight treatments were selected based on a principal component analysis and hierarchical clustering of the target parameters. These selected treatments subsequently underwent a 100 d long‐term trial using lucerne, perennial grass, and perennial grass‐white clover forages. All silages treated with the eight additives exhibited an improved homolactic fermentation and a lower (p &lt; .001) pH, acetic acid, and ethanol content than the control. Although DM losses and ASTA markedly improved in the treatments containing MnSO4 in the short‐term trial, these effects were not observed in the long‐term trial. These outcomes suggest that the selected additives can improve silage fermentation quality across a broad range of forages, but further studies are required to assess the impact of MnSO4 on ASTA.

Effects of a genetically modified corn hybrid with α-amylase and storage length on fermentation profile and starch disappearance of whole-plant corn silage and earlage

Two experiments were conducted to evaluate the effects of a genetically modified corn hybrid with α-amylase expressed in the kernel (AMY) on fermentation profile, aerobic stability, nutrient composition, and starch disappearance of whole-plant corn silage (WPCS) and earlage. Both hybrids, AMY and an isogenic corn hybrid (ISO), were grown in 10 replicated plots (5 for WPCS and 5 for earlage). Samples of each plot were collected at harvest, homogenized, and divided into 5 subsamples which were randomly assigned to 5 storage lengths (0, 30, 60, 90, and 120 d). Both datasets (WPCS and earlage), were analyzed separately as a completely randomized block design in a factorial arrangement of treatments, with a model including the fixed effects of hybrid, storage length, and their interaction, and the random effect of block. Minor differences on fermentation profile were observed between AMY and ISO for WPCS and earlage. An interaction between hybrid and storage length was observed for DM losses in WPCS, where losses were similar at 30, 60 and 90 d, but lower for AMY compared with ISO at 120 d. No effect of hybrid was observed on yeast and mold counts for WPCS or earlage. The aerobic stability of WPCS was greater for AMY than ISO. For earlage, AMY had greater DM losses and aerobic stability than ISO. An interaction between hybrid and storage length was observed for ammonia-N in both WPCS and earlage, where ammonia-N was similar at 0 d but greater for AMY than ISO throughout later storage lengths. A similar interaction was observed for water-soluble carbohydrates (WSC) concentrations in WPCS, where ISO had greater WSC than AMY at 0 d but was similar throughout later storage lengths. However, AMY earlage had a greater WSC concentration throughout storage length, but a lesser magnitude after ensiling. Starch concentration was greater for AMY than ISO in WPCS and earlage. Greater starch disappearances at 0 h and 6 h were observed for ISO in WPCS and earlage. Minor effects on fermentation profile, microbial counts, aerobic stability and nutrient composition suggests that AMY can be ensiled for prolonged periods with no concerns for undesirable fermentation or nutrient losses. However, in situ starch disappearance was lower for AMY compared with ISO.

Effects of particle size and storage length on the fermentation pattern and ruminal disappearance of rehydrated corn grain silage hammer mill processed

The aim of this study is to determine whether the particle size interacts with the storage length to affect the fermentation pattern and the ruminal disappearance of rehydrated corn grain silage (RCGS). It was also assessed whether particle size affects the minimum storage time in relation to the disappearance of ruminal dry matter (DMD). The treatments consisted of six mean particle sizes of RCGS ground in a hammer mill, resulting from different grinding degrees (0.97 mm, 1.11 mm, 1.35 mm, 1.51 mm, 1.69 mm, and 1.75 mm), which were stored for 15, 30, 60, 120, and 240 days. Data were analyzed as repeated measures using the MIXED procedure. When effects of interaction between factors were identified, data were analyzed as polynomial contrasts. Ruminal in situ DM disappearance was modeled across different storage lengths using a non-linear regression model. Prolamin was higher in 1.69 mm and 1.51 mm silages at 15 and 30 days of storage (P < 0.05). Also was observed a decrease in prolamin at 240 days of storage (P < 0.01). The pH of silages was lower after 60 days of storage (P < 0.01), a reduction which is linked to the smaller particle size in 1.11 mm and 1.75 mm silages (P < 0.01). No interaction effects were identified between storage length and DM loss. A greater loss occurred in 1.69 and 1.75 mm silages at 15, 30, and 240 days. At 15 and 60 days, a quadratic behavior was observed, varying according to the increase in particle size. Acetic acid, ethyl lactate, and 1,2 propanediol concentrations increased with storage length (P < 0.01). Lactic acid concentration was affected at 240 days, exhibiting a quadratic behavior according to particle size. The growth pattern of LAB exhibited a quadratic behavior as the mean RCGS particle size increased. The aerobic stability of silages increased after 60 days of storage (P < 0.01), and an interaction effect was found in relation to particle size. According to the Broken-line linear-linear model, maximum disappearance increments were observed at 36, 38, 39, 50, 54, and 46 days of storage for silages with particles measuring 0.97 mm, 1.11 mm, 1.35 mm, 1.51 mm, 1.69 mm, and 1.75 mm, respectively. All silages exhibited an adequate fermentation, and the minimum storage length depended on the particle size of RCGS ground in a hammer mill.

Toward making a high-quality silage from common reed (Phragmites australis).

The effects of the addition of molasses (5 and 10% of dry matter: DM basis; M5 and M10 respectively) with or without urea (2%; U2, U2 + M5 and U2 + M10) on chemical and mineral composition, silage quality, microbial populations, buffering capacity and fermentation/digestion characteristics of common reed (Phragmites australis) ensiled under anaerobic conditions were investigated. The additives changed the silage quality of common reed compared with the control. Crude protein content was significantly (p < 0.0001) increased in urea-containing silages, whereas ash-free neutral detergent fibre concentration decreased in M10 compared with the control (p = 0.05). Treatment with 2% urea (U2) resulted in the reduction of most measured minerals with a severe decrease in iron concentration. The amount of gas produced after 96 h of incubation was, respectively, higher for U2, M10, U2M10, U2M5 and M5 compared with the control (p < 0.0001). Although the greatest in vitro dry matter digestibility and in vitro organic matter digestibility (96 h) were observed in U2 (p < 0.0001), no significant differences were found between U2 and M10. Supplementation with 10% molasses (M10) significantly increased lactic acid concentration, aerobic stability and total bacteria compared with other treatments (p < 0.0001). Moreover, DM loss (p = 0.0004), total yeast and mould (p < 0.0001) were significantly decreased as a result of 10% molasses treatment. Overall, it can be suggested that treating silage with 10% molasses (M10) has the potential to efficiently improve the nutritive value of common reed.

Does Semaglutide Use Decrease Complications and Costs Following Total Knee Arthroplasty?

BackgroundDiabetes mellitus (DM) and obesity are associated with total knee arthroplasty (TKA) complications. Semaglutide, a medication for DM and weight loss, can potentially affect TKA outcomes. This study investigated whether semaglutide use during TKA demonstrates fewer: (1) medical complications; (2) implant-related complications; (3) readmissions; and (4) costs. MethodsA retrospective query was performed using a National database to 2021. Patients undergoing TKA for osteoarthritis with DM and semaglutide use were successfully propensity score-matched to controls semaglutide = 7,051; control = 34,524. Outcomes included 90-day postoperative medical complications, 2-year implant-related complications, 90-day readmissions, in-hospital lengths of stay, and costs. Multivariate logistical regressions calculated odds ratios (ORs), 95% confidence intervals, and P values (P < .003 as significance threshold after Bonferroni correction). ResultsSemaglutide cohorts had higher incidence and odds of myocardial infarction (1.0 versus 0.7%; OR 1.49; P = .003), acute kidney injury (4.9 versus 3.9%; OR 1.28; P < .001), pneumonia (2.8 versus 1.7%; OR 1.67; P < .001), and hypoglycemic events (1.9 versus 1.2%; OR 1.55; P < .001), but lower odds of sepsis (0 versus 0.4%; OR 0.23; P < .001). Semaglutide cohorts also had lower odds of prosthetic joint infections (2.1 versus 3.0%; OR 0.70; P < .001) and readmission (7.0 versus 9.4%; OR 0.71; P < .001), and trended toward lower odds of revisions (4.0 versus 4.5%; OR 0.86; P = .02) and 90-day costs ($15,291.66 versus $16,798.46; P = .012). ConclusionSemaglutide use during TKA decreased risk for sepsis, prosthetic joint infections, and readmissions, but also increased risk for myocardial infarction, acute kidney injury, pneumonia, and hypoglycemic events.

25 Effect of Cyclic Monensin Feeding onin Situ Degradability of Low-Quality Forage Fed to Ruminally Cannulated Beef Steers

Abstract Effects of cyclic monensin feeding on in situ disappearance kinetics of low-quality forage (LQF; 4.9% CP) were evaluated. Ruminally cannulated steers (n = 12; Bos taurus; 260 kg BW) consuming LQF were randomly assigned to of 3 treatments in a completely randomized design: CON (0 mg∙animal-1∙day-1 monensin; Rumensin 90, Elanco Animal Health, Greenfield, IN), 2) MON (200 mg∙animal-1∙day-1 monensin), or 3) CYC (200 mg∙animal-1∙day-1 monensin for 4 d and 0 mg∙animal-1∙day-1 monensin for subsequent 4 d). Steers were fed dried distillers’ grains with solubles (1 kg∙animal-1∙day-1) for monensin inclusion. Four 28-d replicated cycles were used allowing 9 days for treatment adaptation, 4 days for sampling, and 4 days for withdrawal of monensin within the CYC treatment group. Low-quality forage samples were weighed into 0 × 20 cm polyester bags in replicates of four for each sampling hour. On d 0, all replicates were placed into the ventral area of the rumen and replicates were removed 0, 4, 8,6, 24, 48, and 72 hours post feeding. Upon removal, bags were rinsed in ice water, and frozen until final replicates were removed. Subsequently, bags were washed utilizing a commercial washing machine, dried at 55°C to a constant weight and dried residues were analyzed for DM. Ruminal in situ DM degradation data over time were fitted to the first-order exponential model with discrete lag using the NLIN procedure of SAS 9.4, then model parameters were compared with PROC GLIMMIX model to compare dietary treatments with period included as a random variable. There was no effect of treatment (P ≥ 0.22) on potentially degradable DM, rate of DM degradation, DM residue, DM lag, or wash loss of DM. Results are consistent with findings in the concurrent in vivo study conducted by Hook et al. (2022). Further evaluation of NDF degradability is required to determine a clear effect of cyclic monensin feeding on steers consuming an LQF.

Energy-aware message distribution algorithm for enhance FANET pipeline surveillance reliability

Features such as the communication scheme, energy awareness, and task distribution amongst others are the key component that characterizes the Flying Ad-hoc Network (FANET). The operational efficiency in FANET surveying a specific region is affected by the nature of the UAVs' node placement, routing protocol, energy-aware task distribution, and node interaction amongst others. In this paper, Drone 1 (D1), Master Drone (DM), and Drone 2 (D2) were used to survey a pipeline of length 12.2 m. This paper aims at minimising energy use by drones during surveillance using energy-aware node exchange technique, task interaction and distribution scheme for each UAV. Due to fast energy depletion of DM due to packets aggregation, its election is based on the UAV with the highest energy before take-off. For two different simulations, 14,697.0 J and 14,836.6 J were obtained for DM. To avoid system failure due to fast energy loss of DM, the drones swapped positions and status. First swapping command comes up when DM loses 50% of its energy, while the second command occurs when it further loses 15%. Return to base threshold energy is computed for the three UAVs to avoid crash due to insufficient energy during surveillance. DM returns to base threshold energy for both single and double swapping simulation were 658.105 J and 652.456 J respectively. From the results obtained the algorithms were able to exchange nodes to maximize energy usage and perform an interaction-based task distribution for cooperative task sharing during surveillance. This translates into longer surveillance time and effective telemetry data aggregation.

Effect of Length of Storage and Chemical Additives on the Nutritive Value and Starch Degradability of Reconstituted Corn Grain Silage

The aim of this study was to evaluate the feed quality of reconstituted corn grain silage (RCGS), treated with chemical additives and stored for 15, 30, or 60 d in 5-L plastic buckets. Dry ground corn was rehydrated to 350 g·kg−1 and treated with either polysorbate 80 (2 L·t−1) (POL), propionic acid 28% (2 L·t−1) (PRO), Mycoflake™ (2 L·t−1-blend polysorbate 80 and propionic acid) (MYC) or nothing (CON). The effect of the length of storage was combined in a factorial arrangement with the additives. Ammonia-N increased from d-15 of storage. A treatment × storage length interaction was observed for ethanol content at d-60 of storage, and all treatments had lower ethanol concentration than CON. There was an interaction for butyric acid content at d-30 and d-60 of storage; CON showed higher butyric acid concentration than treated silages. Aerobic stability increased from d-15 to d-30. At d-15 of storage, the PRO and MYC treatments decreased the DM losses. The length of storage increased the ruminal in situ degradability of starch, and DM and MYC increased the DM degradability in 3.6 percentage units at 12 h of incubation, compared with POL. In conclusion, increasing the length of storage of the RCGS from d-15 to d-60 improved the starch and DM degradability. Mycoflake increased the availability of nutrients, and the length of storage enhanced the aggregation of particles; further, polysorbate 80 (Tween 80) might be further studied as a potential antimicrobial agent in silages.

Co-Harvest and Anaerobic Co-Storage of Corn Grain and Stover as Biomass Feedstocks

Highlights Cutting height and harvest date were used to alter stover moisture content, yield, and composition. Anaerobic co-storage of grain and stover limited losses to less than 6% of dry matter. Extent of fermentation was greater for higher moisture stover than grain, but total acids were less than 5 g kg-1. Reducing the harvester cutter head rotational speed resulted in a greater fraction of whole corn kernels. Abstract. This research investigated the utility of co-harvesting and anaerobic co-storage of corn grain and stover to positively influence their physical and chemical characteristics as a biomass feedstock. Corn grain and stover were harvested in 2019 and 2020 with a self-propelled forage harvester. Stover yield, moisture content, and composition were altered by the harvest date, stubble height, and header configuration. Harvest date had the utility of varying the stover moisture content (p &amp;lt; 0.001) from 42.3% to 53.5% (w.b.) and 43.1% to 53.9% (w.b.) for the 2019 and 2020 harvest years, respectively. Stubble height was also utilized to vary stover moisture content. A negative linear relationship was established between stubble height and stover moisture content for the early (R2 = 0.76) and late harvest (R2 = 0.91) dates for both years. Stover yield also showed a negative linear relationship (R2 = 0.76) with stubble height over both years. Regardless of the stubble height, the row-crop header collected more stover (p &amp;lt; 0.001) than the ear-snapper header. In 2020, harvested stover ranged from 5.0 to 10.5 Mg ha-1, with ha-1 representing 41% to 85% of the total available stover. In both years, stover ash content was less than 64 g kg-1. Material stored in pilot-scale silos (19 L) was well conserved during anaerobic storage, with average DM losses of 4.8% and 3.4% in 2019 and 2020, respectively. Grain moisture content averaged 23.6% (w.b.) at harvest, and 31.0% (w.b.) after storage as moisture migrated from the moist stover to the drier grain. Harvesting whole-plant corn with a forage harvester had the unwanted effect of reducing the particle size of the grain fraction, which would complicate downstream utilization. However, reducing the harvester cutterhead speed increased the fraction of intact kernels from 47% to 85% by mass. The studied system was a viable alternative to conventional corn grain and stover systems for producing feedstocks for biochemical conversion. Keywords: Ash, Ensiling, Ethanol, Maize.

Applying the benzoate equivalent concept in rehydrated corn grain silage

The benzoate equivalent (BEq) concept is a potential tool to compare silage additives that differ in active ingredients and their respective concentrations. Thus, our objective was to define BEq coefficients for potassium sorbate and sodium propionate in comparison to sodium benzoate, and establish an application rate based on the BEq to extend the aerobic stability of rehydrated corn grain silage. Dry corn kernels were ground (hammer mill, 8-mm screen), rehydrated to 350 g/kg moisture, and treated as follows: no additive (control), potassium sorbate (PS) at 125, 250, 500 and 1000 g/t, sodium benzoate (SB) at 250, 500, 1000 and 2000 g/t, sodium propionate (SP) at 500, 1000, 2000 and 4000 g/t, and a mixture of SB, PS and SP (BPS) at 250 + 150 + 53 g/t, 375 + 225 + 79 g/t, 500 + 300 + 105 g/t and 625 + 375 + 132 g/t, respectively. Treated grain was stored for 60 d in laboratory silos. All additives, alone or mixed, decreased the yeast and mold counts, ethanol and ethyl ester concentrations, and DM loss during fermentation, whereas more water-soluble carbohydrates were preserved in the treated silages. The untreated (control) corn grain silage rapidly deteriorated upon air exposure (∼ 41 h of aerobic stability), and PS, SB, and SP, alone or mixed, linearly improved the aerobic stability with increasing application rate. PS (BEq = 1.99) was more effective than SB (BEq = 1), and SB was more effective than SP (BEq = 0.35) in enhancing the aerobic stability. An application rate of approximately 1400 g/t of BEq could extend the aerobic stability of rehydrated corn grain silage for 3 d over that of untreated silage. In conclusion, the BEq concept is a plausible tool to compare commercial silage additive preparations and define their application rates in corn grain silage.

Pregnancy loss and subsequent risk of prediabetes, diabetes and metabolic syndrome in couples: Tehran lipid and glucose study

BackgroundThere is limited evidence regarding the impact of pregnancy loss on the subsequent risk of metabolic disorders. We aimed to investigate whether history of pregnancy loss is associated with the subsequent risk of prediabetes (pre-DM), diabetes (DM), and metabolic syndrome (METs) among couples.MethodIn this population-based cohort study, 2765 couples with and without history of pregnancy loss and free of DM, pre-DM, and METs at baseline were included and followed for incidents of DM, pre-DM, and METs by 3-year intervals visits from 1999 to 2018. Detailed data of variables was collected using standard questionnaires, interviews, clinical and laboratory assessments. A modified Poisson regression for binary outcome data with a log link function and robust error variance was used to estimate relative risks (RRs) in couples with and without history of pregnancy loss. Both unadjusted and adjusted models were fitted, and effect measures were calculated.ResultDuring a median follow-up of 15 years, females with history of pregnancy loss were experienced more pre-DM (50% vs. 45.5%), DM (28.9% vs. 21.3%), and METs (70% vs. 60.1%) than females without such history. Moreover, history of pregnancy loss increased the risk of METs by 8% among females. The incidence of DM in males with history of pregnancy loss in their spouses was higher than in males without it (28.8% vs. 23.5%). Among males, having a spouse with history of pregnancy loss was positively associated with the risk of pre-DM (RR = 1.12; 95%CI: 1.02, 1.23, p = 0.02); furthermore, they were more prone to the risk of METs than females with a history of pregnancy loss (RR = 1.13; 95%CI: 1.07, 1.20, p < 0.001).ConclusionAlthough pregnancy loss is a female-specific factor, may foreshadow the subsequent METs, our study identified a higher risk of subsequent pre-DM and METs in males with history of pregnancy loss in their spouses. Pregnancy loss could be considered a possible future risk factor for metabolic disorders in couples.

Succession changes of fermentation parameters, nutrient components and bacterial community of sorghum stalk silage.

To better understand the ensiling characteristics of sorghum stalk, the dynamic changes of fermentation parameters, nutrient components and bacterial community of sorghum stalk silage were analyzed by intermittently sampling on day 0, 1, 3, 7, 14, 28, and 56 of ensiling duration. The results showed that high-moisture sorghum stalk was well preserved during ensiling fermentation, with the DM loss of 4.10% and the little difference between the nutrients of sorghum stalk before and after ensiling. The pH value of silage declined to its lowest value of 4.32 by Day 7 of ensiling, and other fermentation parameters kept steady since Day 28 of ensiling. The amplicon sequencing analysis revealed that the alpha diversity parameters of silage bacterial community including Shannon index, observed features, Pielou evenness and Faith PD gradually declined (P < 0.01) with ensiling duration. Principal coordinate analysis (PCoA) revealed that bacterial profiles of raw material would experience a succession becoming a quite different community during ensiling fermentation. Taxonomic classification revealed a total of 10 and 173 bacterial taxa at the phylum and genus level, respectively, as being detected with relative abundances higher than 0.01% and in at least half samples. LEfSe analysis revealed that 26 bacterial taxa were affected by sampling timepoint (P < 0.05 and LDA score > 4). When focusing on the dynamic trend of silage bacterial taxa, lactic acid bacteria successfully dominated in the bacterial community on Day 1 of ensiling, and the bacterial community almost came to a plateau by Day 28 of ensiling, with Lactobacillus and Leuconostoc as the dominant genera. In a word, the succession of fermentation parameters, nutrient components and bacterial community indicate a successful dominance establishment of LAB and a fast advent of fermentation plateau, suggesting that high-moisture sorghum stalk can be ensiled directly, but the pH of mature silage is a little high.

Characterization of bacterial community and fermentation parameters during ensiling of Napier grasses harvested at various times within a day

Grasses exhibit variations in chemical composition and epiphytic microbiota during daylight. These variations may influence the natural fermentation parameters of silage. This study was to evaluate the bacterial community and fermentation parameters during ensiling of Napier grasses harvested at various times within a day. Secondary growth of Napier grass (vegetative stage) was harvested at 7:00 (AM), 12:00 (M) and 17:00 (PM) on three sunny days. The Napier grasses were ensiled in polyethylene plastic bags and bags were opened after 1, 3, 7, 14, 30 and 60 days. Compared with AM-cut fresh Napier grasses, M and PM-cut fresh Napier grasses had higher (P < 0.05) dry matter, and lower (P < 0.05) crude protein contents and buffering capacity. The lactic acid bacteria (LAB) and aerobic bacteria counts decreased (P < 0.05) with the delay of harvest time. Compared with AM silage, M and PM silages exhibited slower pH declines and WSC consumptions plus less lactic acid production during ensiling. After 7 days of ensiling, propionic acid contents and DM losses tended to be higher (P < 0.05) in M and PM silages compared with AM silage. The M and PM-cut fresh Napier grasses had higher (P < 0.05) bacterial diversity and richness than AM-cut fresh Napier grasses. During ensiling, Lactobacillus gradually dominated the microbiota in AM silage and its relative abundance reached 49.1% at the end of ensiling. By contrast, Klebsiella and Enterobacter tended to flourish in the M and PM silages as ensiling progressed. Analysis of the LAB community showed that M and PM-cut fresh Napier grasses had higher (P < 0.05) relative abundance of Leuconostoc and lower (P < 0.05) Lactococcus compared with AM-cut fresh Napier grasses. On day 3 of ensiling, the relative abundance of Leuconostoc and unclassified_f_Leuconostocaceae were higher (P < 0.05), and Lactococcus and unclassified_o_Lactobacillale were lower (P < 0.05) in M and PM silages as opposed to those in AM silage. After 14 days of ensiling, M and PM silages contained higher Pediococcus and Enterococcus compared with AM silage. These results revealed that Napier grasses harvested at various times within a day had different fermentation parameters and bacterial community during ensiling. In practice, diurnal changes in Napier grass could be an important factor to consider for producing high-quality silage.

Effects of sodium diacetate or microbial inoculants on aerobic stability of wilted rye silage.

The primary goal was to identify the effectiveness of chemical or biological additives in delaying the deterioration of early-harvested wilted rye silage after exposure to air. Rye harvested as a whole plant at the early heading stage was wilted for 24 h. The wilted forage was divided into treatments including sodium diacetate (SDA) at 3 (SDA3) and 6 g/kg (SDA6), Lactobacillus plantarum (LP), L. buchneri (LB), or their equal mixture (LP+LB) at 1×106 colony-forming unit/g fresh matter. After 60 d of conservation in 20-L silos, lactic acid was greater in LP and LP+LB silages than other treatments (102 vs 90.2 g/kg dry matter [DM]). Acetic acid was greatest in SDA6 (32.0 g/kg DM) followed by LB (26.1 g/kg DM) and was lowest in LP treatment (4.73 g/kg DM). Silage pH was lower with microbial inoculation and the lowest and highest values were observed in LP and untreated silages, respectively. After 60 d, neutral detergent fiber concentration was lowest in SDA6 silages, resulting in the greatest in vitro DM digestibility (846 g/kg DM). Aerobic stability was longest in SDA6 (176 h) followed by LB treatment (134 h). Instability after aerobiosis was greatest in LP silages (68 h), about 8 h less than untreated silages. After aerobic exposure, yeast and mold numbers were lowest in SDA6 silages, resulting in DM loss minimization. Exhaustion of acetic acid and lactic acid after aerobic exposure was lowest with SDA6 but greatest with untreated and LP silages. Treatment of early-cut wilted rye forage with SDA at 6 g/kg resulted in silages with higher feeding value and fermentation quality, and substantially delayed deterioration after aerobic exposure, potentially qualifying SDA at this load for promotion of silage quality and delaying aerobic spoilage of early-harvested (low DM) rye forage.

Effect of lignosulfonates on the dry matter loss, nutritional value, and microbial counts of high moisture alfalfa silage

In this experiment, we evaluated the effects of sodium lignosulfonate (NaL) and magnesium lignosulfonate (MgL) applied independently at 0, 5, 10, and 15 (g/kg, fresh weight basis) and an inoculant (INO) on high-moisture (DM: 219 g/kg, fresh weight basis) alfalfa (Medicago sativa L.) silage nutrient preservation. The INO consisted of Pediococcus pentosaceus and Lactobacillus plantarum applied at a rate of 4.95 and 4.00 log cfu/g (fresh weight basis), respectively. Data were analyzed as a randomized complete block design (RCBD; 5 blocks) and linear and quadratic polynomial contrasts were used to determine dose rate effects for NaL and MgL and orthogonal contrasts for INO effects. At d 0, increasing NaL dose from 0 to 15 g/kg increased linearly DM (219–227 g/kg) and decreased linearly mold counts (4.48–3.62 log cfu/g; fresh weight basis). No treatment effects were observed on lactic acid bacteria counts (7.04 log cfu/g; fresh weight basis), water soluble carbohydrates (61.5), and aNDF (432 g/kg of DM). After 229 d of ensiling, both MgL and INO increased DM loss (x¯= 13.7 and 13.7 vs 11.3% of DM) due to a lower production of lactic acid (x¯= 75.5 and 78.3 vs 92.3 g/kg of DM, respectively) which resulted in a higher pH relative to untreated silage (x¯= 4.41 and 4.46 vs 4.33; respectively). Increasing doses from 0 (6.42) to 15 g/kg for both NaL (6.90) and MgL (7.06 log cfu/g; fresh weight basis) resulted in linear and quadratic increases of lactic acid bacteria counts, respectively. These counts were also higher in INO treated silages (6.98 log cfu/g; fresh weight basis), relative to untreated silage. Furthermore, both NaL and MgL decreased in vitro ruminal total volatile fatty acid concentration (x = 87.0 and 93.1 vs 97.1 mM; respectively), compared with the untreated silage. The greater acidification in untreated silage prevented additives tested from reducing the generation of NH3-N (x¯= 110 g/kg of N) during ensiling. Overall, none of the additives tested improved the preservation of high-moisture alfalfa silage nutrients beyond what was observed in untreated.

1485-PUB: Assessing Correlates of Microvascular Complications among Patients with Diabetes Mellitus

Background: Microvascular complications associated with diabetes (DM) are important predictors of morbidity and mortality. Understanding the factors associated with these complications is important in reducing their burden. Method: Using a cross-sectional study design, seventy-one ambulatory patients with Type 2 DM (female 60.6%, white 36.6%, mean age 64.1± 10.3 years, and mean duration of DM 15.8±9.1 years) were recruited using an investigator-administered questionnaire and chart review. Variables of demographics, smoking, HbA1c, DM duration, and complications were collected. CRP was measured and oral health status was assessed by a clinical exam. Data were analyzed using Chi-square/Fisher exact tests and binomial logistic regression (SPSS version 27) . Results: A larger proportion of patients with neuropathy, retinopathy or nephropathy were completely edentulous compared to those without these complications (45.3% vs. 10.3%; p=0.002) . The likelihood of having microvascular complications based on selected independent variables is shown in the table. Conclusions: In this DM patient sample, advancing age and being completely edentulous were significantly associated with an increased likelihood of having microvascular complications. Our findings may reflect some similarities between underlying risk factors that contribute to microvascular complications of DM and tooth loss. Disclosure K.Izuora: Research Support; Novo Nordisk. A.Alver: None. A.Basu: None. K.Batra: n/a. S.J.Williams: None. J.L.Ebersole: None.