Diversity Of Barley Landraces Research Articles

Evaluation of Genetic Diversity in Barley Landraces through Agro-Morphological and Biochemical Characterization

| Genetic diversity can be investigated through different methods such as morphological traits, biochemical (SDS PAGE) and molecular markers. The present work was conducted on the basis of morphological and biochemical characterization in order to estimate the genetic diversity among the barley landraces; to explore significant variation which can be used in breeding programs. For this purpose, 40 barley landraces were collected from Dir Lower and Swat, districts of KP, Pakistan. A total of 18 traits were recorded, 10 qualitative characters including spike density, awn barbs, glume awn, lemma type and awn color, length of the rechilla hairs, lemma color, auricle pigmentation, photoperiod sensitivity and stem pigmentation. Variation was observed in these qualitative traits through frequency distribution. In quantitative traits maximum variation was found for plant height ranging from 36cm to 50cm, days to flowering range from 130 to 133 days), while 100 seed weight ranged from 36 to 86 g and plant biomass range from 50 to 199.5 g per plant. SDS-PAGE analyses of total seed storage protein resulted in a total of 18 polymorphic bands. Total genetic diversity on the basis of total seed storage protein analysis was 17.5%. In Band, the 14 total genetic diversity was (0.60%) followed by Band 16 (0.58%) and Band 17 (0.55%). Similarly, Band 3 showed diversity, while Band 8, 4 and 5 indicated 0.45, 0.38 and 0.35%, respectively. A cluster dendrogram tree was constructed which was divided into two linkage and further divided into seven clusters. The variation was found among the clusters. The information obtained from the study is useful in planning further crop improvement.

English

Barley is one of the major cereal crops grown in Ethiopia. The diversity of barley landraces kept for generations in Ethiopia is nowadays subject to genetic erosion. This is true in North Gondar highlands of the country as well. This research was therefore initiated with the aim of studying the farm diversity status of barley landraces in Debark, Dabat and Wogera districts. A total of 180 randomly selected farmers from six villages were surveyed with a proportion of 30 farmers/village. Ecological models were employed to analyze the level of diversity. Genetic erosion models were employed to estimate the level of genetic erosion over a ten-year period of time. A total of 24 landraces were described by farmers of the studied sites. Of these, 18 of them are still under cultivation although their area coverage is declining from time to time. The landraces Abat gebs, Nech gebs and Tikur gebs were found to be the most common and widely grown. Debark district was found to have the highest richness (Margalef=2.45; Menhinick=1.43) followed by Wogera and Dabat districts. With regard to evenness as a measure of Shannon diversity index, Wogera district (E=0.85) showed the highest diversity followed by dabat (E=0.83) and debark (E=0.79). Simpson’s diversity index (D) also revealed the abundance of Nech gebs (0.66), Abat gebs (0.6) and Tikur gebs (0.52). The landraces Demo kises, Goreneje, Chankirme, Gabieaswelik, Amedo and Gero tal were found to be out of production in the last ten years and probably eroded. Genetic erosion and genetic integrity over ten years (2006-2016) was found to be 25 and 75%, respectively. The name given to landraces studied was found to be associated with certain characteristics or situations. Thus, policy makers and researchers should give attention to conservation of landraces of barley (Hordeum vulgare L.) for better use of genetic resources. Key words: On-farm diversity, genetic erosion, barley, landrace.

Genetic diversity in barley landraces (Hordeum vulgare L. subsp.vulgare) originated from Crescent Fertile region as detected by seed storage proteins.

Genetic diversity in barley landraces (Hordeum vulgare L. subsp.vulgare) originated from Crescent Fertile region as detected by seed storage proteins.

Dissection of barley landraces originated from twelve different countries by using simple sequence repeats markers

Genetik çeşitliliğin önemli bir kaynağı olan yerel çeşitler, tahıl türlerinin geliştirilmesi için önemlidirler. Yerel çeşitler arasındaki genetik çeşitliliğin araştırılması, genetik kaynakları koruma ve arpa ıslahında gelecek stratejileri geliştirme için önemlidir. Bu çalışmada, on iki ülkeden köken alan arpa yerel çeşitlerindeki genetik çeşitlilik, basit sıra tekrarları (BST) işaretleyicileri kullanılarak çalışılmıştır. Yedi arpa bağlantı grubuna ait onaltı BST işaretleyicisi yüksek genetik çeşitliği ortaya çıkarmıştır. Toplamda 92 polimorfik allel sayılmış ve allel sayısı 5.75 ortalama ile lokus başına 1 ila 7 arasında olmuştur. Genetik çeşitlilik en yüksek Türkiye yerel çeşitlerinde (0.66 ± 0.13), en düşük Ukrayna yerel çeşitlerinde (0.38 ± 0.24) belirlenmiştir. Polimorfik lokus yüzdesi, Almanya, Holanda, Rusya, Türkiye ve Amerika’da en yüksek yani % 100 iken, % 75 ile en düşük İngiltere’de bulunmuştur. Yerel çeşitlerin kümeleme analizleri onları dört ana gruba ayırmıştır. Sonuçlar gelecekteki ıslah çalışmaları için farklı ülkelerden arpa yerel çeşitleri hakkında ilave genetik bilgiler sağlamıştır.

Dissection of Barley Landraces Originated From Twelve Different

Landraces, as an important source of genetic diversity, are important for improvement of crop species. Investigating of genetic diversity among landraces is necessary to conserve genetic resources and develop future strategies on barley breeding. In this study, genetic diversity in barley landraces originating from twelve countries was studied using simple sequence repeat (SSR) markers. Sixteen SSR markers belong to the seven barley linkage groups revealed high genetic diversity. A total of 92 polymorphic alleles were scored and the number of alleles ranged from 1 to 7 per locus, with an average of 5.75. Genetic diversity was the highest in landraces from Turkey (0.66 ± 0.13) and the lowest in those from Ukraine (0.38 ± 0.24). While the highest percentage of polymorphic loci was found for landraces from Germany, Netherland, Russia, Turkey and USA as 100%, the lowest one was in England with 75%. Clustering analysis of landraces divided them into four main groups. The results provided additional genetic information about the barley landraces from different countries for future breeding process.

English

Barley (Hordeum vulgare L.) has great adaptability to a wide range of environments. To determine genetic diversity in barley landraces, a total of 43 landrace populations were randomly sampled from the farmers’ field on plant basis and characterized for eight qualitative traits; namely, kernel row number, spike density, lemma awn barb, glume color, lemma type, length of rachila hair, kernel covering and lemma/kernel color. Morphological diversity was determined by the Shannon-Weaver index (H’). Overall barley landrace populations showed an average diversity index of 0.59, implying large diversity for the populations. Selection for adaptation to different altitude classes appears to be the main factor that has determined the observed variation, along with population-size effects. The result showed that barley landraces from Gamo highlands, Ethiopia are constituted by highly variable landraces that have large within-population diversity. These landraces are also shown to be locally adapted, with the major driving force that has shaped their population structure being consistent with selection for adaptation along an altitudinal gradient. Overall, this study highlights the potential of such landraces as a source of useful genes that can be exploited in crop improvement programmes. Key words: Barley, characters, landraces/farmer varieties, morphological diversity, altitudinal gradient.

Study of the genetic diversity of Korean, Chinese and Japanese landraces of barley (Hordeum vulgare L.) using microsatellites

Study of the genetic diversity of Korean, Chinese and Japanese landraces of barley (Hordeum vulgare L.) using microsatellites Barley (Hordeum vulgare L.) is one of important winter cereals in the world and has been the subject of numerous genetic investigations. Studies of its genetic diversity based on germplasm have a significant impact on crop breeding and conservation of genetic resources. This study was conducted to reveal the genetic diversity in barley landraces from Korean, Chinese and Japanese populations and evaluate the discrimination ability of SSR markers, distributed uniformly throughout the barley genome. Seven SSR primers were used to screen a set of 737 diverse barley landraces from Korea, China and Japan. The observed number of alleles per locus (Na), the effective number of alleles (Ne), and the mean gene diversity (He) were 11.14, 3.6245 and 0.7048, respectively. The number of alleles per locus was highest in Chinese landraces (8.9 alleles), followed by Korean (8.6) and Japanese (6.4). In this regard, HVKASI primer may be useful to distinguish Japanese landraces from others, as this unique allele was only detected at 175 bp in Japanese landraces. The average value of genetic distance was D=0.935. The largest genetic distance (D=1.209) among the three regional (representing each country in general) populations was found between Korean and Japanese populations. Based on the UPGMA dendrogram, four major groups can be distinguished at the similarity value of 0.43. The scatter plot showed overlapping in the central part amongst 3 groups of barley landraces. SSR markers are a powerful tool to examine functional diversity. Rich barley gene pool can be very useful for meeting current and future challenges in barley raising.

Genetic diversity in barley landraces from Syria and Jordan

Single-head progenies derived from barley landraces collected along the Fertile Crescent in Syria and Jordan were evaluated for agronomic, morphological, and quality traits in a typical barley growing area in Northern Syria. A large diversity was observed both between and within collection sites, and in most cases the variation was useful for breeding purposes. Single plant progenies were identified with larger yields and more desirable expressions of agronomic characters than the original landraces. The utilization of this material in a breeding program for dry areas is discussed.