Diurnal Moths Research Articles

Regulation of opsin and circadian clock genes on mate-finding behavior of the day-flying red moth, Phauda flammans (Walker)

ABSTRACT First, significantly higher mate-finding success was found under light condition than under constant darkness condition in Phauda flammans, a typical diurnal moth. We speculate that mate-finding behavior in P. flammans may be influenced by the light-sensitive opsin genes Long wavelength opsin (PfLW), Ultraviolet opsin (PfUV) and Blue opsin (PfBL), which are potentially regulated by both light-cues and endogenous circadian rhythms. Second, the circadian clock genes Period (PfPer), Timeless (PfTim), Cryptochrome1 (PfCry1), Cryptochrome2 (PfCRY2), Cryptochrome3 (PfCry-like), Clock (PfClk), Cycle (PfCyc), Vrille (PfVri), and Slimb (PfSli) were identified in P. flammans. Third, circadian rhythms in the relative expression levels of opsin and circadian clock genes were demonstrated via quantitative real-time PCR analysis, with peak expression coinciding with the mate-finding peak. Notably, the relative expression of PfLW in males P. flammans was significantly higher than that in females P. flammans at the mate-finding peaks Zeitgeber time (ZT) 8 and ZT 10 under light, while the expression of the opsin gene PfBL showed a similar pattern at ZT 10 under light. Additionally, the expression of the clock gene PfCry-like was significantly higher in males than in females at ZT 8 and ZT 10 under light, while PfPer, PfTim, PfClk and PfCyc exhibited similar male-biased expression patterns at ZT 10 under light. Conversely, PfCry1 and PfVri expression was significantly higher in females than in male at ZT 8 under light. In conclusion, sex differences were detected in the expression of opsin and circadian clock genes, which indicated that light-mediated regulation of these genes may contribute to the daytime mate-finding behavior of P. flammans.

Functional characterization of sex pheromone receptors PflaOR29 and PflaOR44 involved in the chemoreception of a diurnal moth, Phauda flammans (Walker) (Lepidoptera: Phaudidae)

Recognition of sex pheromones released by heterosexual moths via sex pheromone receptors is key for establishing mating connections in moths. The day-flying moth Phauda flammans is an oligophagous pest in southern cities of China and Southeast Asian countries. Our previous study reported that male P. flammans can be attracted to two sex pheromone compounds [Z-9-hexadecenal and (Z, Z, Z)-9,12,15-octadecadienal] released by females in the field; however, the mechanism of olfactory recognition is not clear. In this study, two sex pheromone receptor genes (PflaOR29 and PflaOR44) were cloned. Among the different tissues, both PflaOR29 and PflaOR44 were highly expressed in the antennae of mated male adults. At different developmental stages, the expression levels of PflaOR29 and PflaOR44 were significantly greater in mated male adults than other stages. The fluorescence signals of PflaOR29 and PflaOR44 were mostly distributed on the dorsal side of the antennae, with a large number of trichoid sensilla. The results of the gene function of PflaOR29 and PflaOR44 based on a Drosophila empty neuron heterologous expression system indicated that PflaOR29 strongly responded to (Z, Z, Z)-9,12,15-octadecadienal but not to Z-9-hexadecenal, whereas PflaOR44 did not respond to the two sex pheromones. Our findings clarify the sex pheromone receptor gene corresponding to (Z, Z, Z)-9,12,15-octadecatrienal. These results provide essential information for analyzing the mechanism of sexual communication in diurnal moths and for identifying target genes for the development of efficient attractants.

First comprehensive higher level phylogeny of Zygaenidae (Lepidoptera) including estimated ages of the major lineages and a review of known zygaenid fossils

AbstractZygaenidae, also known as burnet, forester, smoky, or leaf skeletonizer moths, are a family of mainly diurnal moths well known for their aposematic colouration and the ability to release hydrogen cyanide as a defence mechanism. So far, few attempts have been made to understand the evolutionary history of the global zygaenid fauna. Here, we inferred the most comprehensive molecular phylogeny for Zygaenidae to date and estimated the lineage timing‐of‐divergence with a Bayesian approach. Building on earlier work, we significantly increased the taxon and gene sampling for the family, which here included data from 30 gene fragments, recovered from public databases or newly sequenced, for almost 30% of the species representing 92 genera (49%) and all five subfamilies. We recovered strong support for the monophyly of Zygaenidae, Chalcosiinae, and Zygaeninae. Procridinae were recovered as monophyletic with low support, whereas the monophyly of Callizygaeninae remains untested as we sampled only one of the two genera. In the core dataset, we recovered Procridinae as sister to Callizygaeninae + Chalcosiinae. This large clade is the sister lineage to Zygaeninae. The position of Inouelinae could not be resolved. The lineage leading to the extant Zygaenidae appears to have diverged in Late Cretaceous (ca. 86 Ma), while the divergence among the subfamilies occurred several million years before the Cretaceous–Paleogene mass extinction event (ca. 66 Ma). Additionally, we provide a review of known fossil Zygaenidae as Appendix S1. Our results form a strong basis for future studies of zygaenid biosystematics, including their ecology, evolution, and behaviour.

Pupal warning color development in above‐ground pupating species but cryptic color in ground‐surface pupating species of the nine‐spotted diurnal moths (Erebidae: Arctiinae: Syntomini)

AbstractInsects usually have cryptic colors to avoid detection by visually hunting predators. However, if the insects acquire toxic or repellent substances against predators, some of them develop conspicuous coloration to exhibit their unpalatability. Such warning colors allow insects to survive. In the nine‐spotted diurnal moths (Erebidae: Arctiinae: Syntomini), we found the above‐ground pupating species to have conspicuous colored pupae, but the ground‐surface pupating species to have cryptic colored pupae. In this study, the relationships between unpalatability and coloration of these pupae are examined among three species of Amata and one species of Syntomoides. Pupae of the two species (A. germana and A. flava) are conspicuous in their color pattern with seven black dotted lines longitudinally on their pale‐yellow bodies. These pupae are exposed to the aerial predators in a coarse silk mesh hanging from leaves and/or branches. The other two species (A. fortunei and S. imaon) pupate in spaces under stones, fallen twigs and leaves on the ground surface, and the pupae in a coarse silk cocoon is cryptic dark brown. Their pupation site selections are reproduced in the rearing glass vessels. Palatability assessment using lizards as a potential predator suggests that pupae of A. germana, A. flava and A. fortunei are unpalatable and the lizard's feeding response decreases with experience. However, pupae of S. imaon are all eaten (palatable). Finally, the possible evolutionary scenario of pupal colors of these four species is discussed in relation to pupation site selection and palatability.

First record of the rare moth Amana angulifera Walker, 1855 (Lepidoptera: Epicopeiidae) from India since the 19th century

Amana angulifera Walker, 1855, a diurnal moth belonging to the family Epicopeiidae, was described from India based on a previously collected specimen. Though type locality was omitted in the original description, in the last decade of the 19th century, the habitat of the moth was mentioned to be Khasi Hills of the present Meghalaya state of India. It is also recorded from Yunnan in southern part of China. After a gap of more than a century, this paper reports the presence of the species again from India, this time in the Himalayan state of Arunachal Pradesh, bordering China, filling a sampling gap within its distribution range. Further study of the moth in this region is recommended to fill the sampling gaps and assess its life cycle, population trends, abundance, phenological responses and inter-specific interactions with plants so that conservation measures may be taken up accordingly.

Molecular Characterization and Expression Patterns of Two Pheromone-Binding Proteins from the Diurnal Moth Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae).

Sex pheromone-binding proteins (PBPs) play an important role in sex pheromone recognition in Lepidoptera. However, the mechanisms of chemical communication mediating the response to sex pheromones remain unclear in the diurnal moths of the superfamily Zygaenoidea. In this study, Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae) was used as a model insect to explore the molecular mechanism of sex pheromone perception in the superfamily Zygaenoidea. Two novel pheromone-binding proteins (PflaPBP1 and PflaPBP2) from P. flammans were identified. The two pheromone-binding proteins were predominantly expressed in the antennae of P. flammans male and female moths, in which PflaPBP1 had stronger binding affinity to the female sex pheromones Z-9-hexadecenal and (Z, Z, Z)-9, 12, 15-octadecatrienal, PflaPBP2 had stronger binding affinity only for (Z, Z, Z)-9, 12, 15-octadecatrienal, and no apparent binding affinity to Z-9-hexadecenal. The molecular docking results indicated that Ile 170 and Leu 169 are predicted to be important in the binding of the sex pheromone to PflaPBP1 and PflaPBP2. We concluded that PflaPBP1 and PflaPBP2 may be responsible for the recognition of two sex pheromone components and may function differently in female and male P. flammans. These results provide a foundation for the development of pest control by exploring sex pheromone blocking agents and the application of sex pheromones and their analogs for insect pests in the superfamily Zygaenoidea.

Diversity and Habitat Preferences of Moths (Insecta: Lepidoptera) in Indikadamukalana, a Lowland Wet Zone Forest in Sri Lanka

The moths (Insecta, Lepidoptera) of Sri Lanka have not been well studied and no comprehensive scientific study on their diversity has been carried out over the past 100 years. Thus, establishing species richness and diversity of moths in different habitats of the island is important. The present study was carried out to investigate moth species diversity and habitat factors which can affect moths in Indikadamukalana Forest Reserve situated in the wet zone of Sri Lanka. Moth diversity of the forest was investigated for a period of approximately seven months in 2018, where two habitat types as forest edge and within forest were studied. Transect line count method was used to sample diurnal moths and light traps were used to study nocturnal moths. Aerial nets and fruit-baited traps were used to improve the sampling efficiency. Weather parameters of the two habitat types were estimated using a potable weather station. A total of 138 moth species of 19 families were recorded, with forest edge habitat recording 18 families (91 species) and within the forest habitat recording 15 families (47 species). Crambidae and Erebidae were the most prominent families of moths found in both habitats of the forest. Gelechiidae was recorded only from within the forest habitat while Sphingidae, Hepialidae, Tortricidae and Bombycidae were recorded only from the forest edge habitat. Weather parameters between the two habitats did not depict a significant difference. Maintenance of Sri Lanka’s biodiversity hotspot status lies within the wet zone of the country. Thus, long term studies of moth communities of the wet zone are of vital importance

Nectar robbing by bees on the flowers of Volkameria inermis (Lamiaceae) in Coringa Wildlife Sanctuary, Andhra Pradesh, India

Floral traits that shape the floral architecture are important to allow or disallow flower visitors to access nectar and effect pollination. Specialization in floral architecture is vulnerable to flower visitors that exploit nectar by robbery without effecting pollination. In Coringa Wildlife Sanctuary in Andhra Pradesh, India, studies on the exploitation of nectar by robbery in plant species with specialized flowers are completely lacking. We carried out a study on the foraging activity of insect foragers and nectar robbing by bees on the specialized nectariferous flowers of an evergreen shrub, Volkameria inermis growing in the landward side of this sanctuary. Field observations indicated that the flowers of this species facilitate legitimate probing only by butterflies and diurnal moths which while seeking nectar effect pollination. However, two bee species Anthophora dizona and Xylocopa pubescens seek nectar illegitimately as primary nectar robbers by making a slit/hole into the corolla tube from outside bypassing the flower front. Additionally, A. dizona gathers pollen legitimately from the stamens which are exposed and placed outside the corolla tube. The stigma is also placed outside the corolla tube but this bee indiscriminately makes attempts to collect pollen from the stigma, as a result of which pollination occurs. Nectar robbing by these bees leads to a reduction in nectar volume in robbed flowers and brings about variability in the standing crop of nectar. As a result, the pollinating butterflies increase the number of nectar foraging visits and shuttle between populations of V. inermis in quest of more nectar to meet their daily metabolic requirements. Such a foraging behavior increases pollination rate in general and cross-pollination in particular, which in turn increases plant fitness in V. inermis. Therefore, the nectar robbing by bees appears to have a positive effect on plant fitness through change in seed set rates.

Multi-gene phylogeny of North American clear-winged moths (Lepidoptera: Sesiidae): a foundation for future evolutionary study of a speciose mimicry complex.

Sesiids are a diverse group of predominantly diurnal moths, many of which are Batesian mimics of Hymenoptera. However, their diversity and relationships are poorly understood. A multi-gene phylogenetic analysis of 48 North American sesiid species confirmed the traditional taxonomic tribal ranks, demonstrated the paraphyly of Carmenta and Synanthedon with respect to several other genera and ultimately provided minimal phylogenetic resolution within and between North American and European groups. Character support from each gene suggested inconsistency between the phylogenetic signal of the CAD gene and that of the other four genes. However, removal of CAD from subsequent phylogenetic analyses did not substantially change the initial phylogenetic results or return Carmenta and Synanthedon as reciprocally monophyletic, suggesting that it was not impacting the overall phylogenetic signal. The lack of resolution using genes that are typically informative at the species level for other lepidopterans suggests a surprisingly rapid radiation of species in Carmenta/Synanthedon. This group also exhibits a wide range of mimicry strategies and hostplant usage, which could be fertile ground for future study.

Identification of Chemosensory Genes, Including Candidate Pheromone Receptors, in Phauda flammans (Walker) (Lepidoptera: Phaudidae) Through Transcriptomic Analyses.

Olfactory and gustatory systems play an irreplaceable role in all cycles of growth of insects, such as host location, mating, and oviposition. Many chemosensory genes in many nocturnal moths have been identified via omics technology, but knowledge of these genes in diurnal moths is lacking. In our recent studies, we reported two sex pheromone compounds and three host plant volatiles that play a vital role in attracting the diurnal moth, Phauda flammans. The antennal full-length transcriptome sequence of P. flammans was obtained using the Pacbio sequencing to further explore the process of sex pheromone and host plant volatile recognition in P. flammans. Transcriptome analysis identified 166 candidate olfactory and gustatory genes, including 58 odorant-binding proteins (OBPs), 19 chemosensory proteins (CSPs), 59 olfactory receptors (ORs), 16 ionotropic receptors (IRs), 14 gustatory receptors (GRs), and 2 sensory neuron membrane proteins (SNMPs). Subsequently, a phylogenetic tree was established using P. flammans and other lepidopteran species to investigate orthologs. Among the 17 candidate pheromone receptor (PR) genes, the expression levels of PflaOR21, PflaOR25, PflaOR35, PflaOR40, PflaOR41, PflaOR42, PflaOR44, PflaOR49, PflaOR51, PflaOR61, and PflaOR63 in the antennae were significantly higher than those in other non-antennae tissues. Among these PR genes, PflaOR21, PflaOR27, PflaOR29, PflaOR35, PflaOR37, PflaOR40, PflaOR42, PflaOR44, PflaOR60, and PflaOR62 showed male-biased expression, whereas PflaOR49, PflaOR61, and PflaOR63 revealed female-biased expression. The functions of related OR genes were also discussed. This research filled the gap of the chemosensory genes of P. flammans and provided basic data for future functional molecular mechanisms studies on P. flammans olfaction.

Butterflies' biodiversity of Souk Ahras Forest (Mechroha and Ouled Driss North east of Algeria)

AbstractThis study investigated for the first time species richness, abundance of butterflies and disturbed habitats in Souk Ahras Forest. The assessment of butterflies' diversity and relative abundance was conducted from July 2019 to July 2021. We recorded 60,696 individuals of 49 butterfly species belonging to six families (Papilionidae, Pieridae, Lycaenidae, Nymphalidae, Hesperiidae and Sphingidae). Nymphalidae was dominant by 19 species, followed by Lycaenidae 13 species, Pieridae 10 species, Hesperiidae 4 species, Papilionidae 2 species and diurnal moths ‘Sphingidae’ with only one species. The highest number of species was recorded in oaks groves (medium high altitude) with 35 species; in terms of abundance, grassland (low altitude) dominates in number (n = 20,094). The lowest species diversity (S = 7) and number of individuals (n = 1227) were recorded in the Dense tree stratum scrub station which records significant water pollution. We notice that the abundance of butterflies was important during the spring and summer periods but less important during the winter. Landscape composition and habitat influence butterfly diversity and their dynamics. Many species used more than one distinct habitat type, or just one special habitat; we suggest that vegetation cover is an important mechanism for butterfly diversity persistence in forest ecosystems mosaics.

The highly invasive Siam Weed, Chromolaena odorata (L.) King and Robinson (Asteraceae), as a seasonal prime nectar source for butterflies (Lepidoptera: Papilionoidea) and other insects (Insecta: Lepidoptera, Hymenoptera, Coleoptera) in West Africa

During mass-blooming in the dry season, the highly invasive Siam Weed, Chromolaena odorata, appears to be a prime nectar source for butterflies and a range of diurnal moths and other insects in West Africa. About 10 % of the West African butterfly fauna were recorded visiting C. odorata flowers between Sierra Leone and Western Cameroon as a result of opportunistic observations between 2010 and 2021. Predators on flower-visiting insects, such as crab-spiders and Flower Mantises, also seem to have become adapted to the newly available food-source. These records indicate that beyond the well-known adverse effects of C. odorata invasion to regeneration of natural vegetation on disturbed ground and the exposure of natural rainforest habitats to wildfires because of the plant’s susceptibility to fire, the diet shift of a considerable proportion of pollinators could imply further threats to biodiversity, such as reducing the reproductive rate of forest plants pollinated by butterflies. Specific studies further targeting the subject are urgently needed.

Insect taxonomy can be difficult: a noctuid moth (Agaristinae: Aletopus imperialis) and a geometrid moth (Sterrhinae: Cartaletis dargei) combined into a cryptic species complex in eastern Africa (Lepidoptera).

The systematic position of a large and strikingly coloured reddish-black moth, Cartaletis dargei Herbulot, 2003 (Geometridae: Sterrhinae) from Tanzania, has remained questionable since its description. Here we present molecular and morphological evidence showing that Cartaletis dargei only superficially resembles true Cartaletis Warren, 1894 (the relative name currently considered a junior synonym of Aletis Hübner, 1820), which are unpalatable diurnal moths superficially resembling butterflies, and that it is misplaced in the family Geometridae. We transfer it to Noctuidae: Agaristinae, and combine it with the genus Aletopus Jordan, 1926, from Tanzania, as Aletopus dargei (Herbulot, 2003) (new combination). We revise the genus Aletopus to contain three species, but find that it is a cryptic species complex that needs to be revised with more extensive taxon sampling. Our results demonstrate the difficulties in interpreting and classifying biological diversity. We discuss the problems in species delimitation and the potential drivers of evolution in eastern Africa that led to phenotypic similarity in unrelated lepidopteran lineages.

Direct and Ambient Light Pollution Alters Recruitment for a Diurnal Plant-Pollinator System.

Artificial light at night (ALAN) functions as a novel environmental stimulus that has the potential to disrupt interactions among species. Despite recent efforts to explain nocturnal pollinators' responses to this stimulus, the likelihood and associated mechanisms of attraction toward artificial light and potential consequences on fitness for diurnal pollinators are still largely unclear. Here, we took advantage of the obligate mutualism between yucca moths (Tegeticula maculata maculata) and yucca plants (Hesperoyucca whipplei) to understand how direct light exposure and skyglow can influence a pairwise plant-pollinator interaction. To surmise whether adult moths exhibit positive phototaxis, we deployed a set of field-placed light towers during the peak of yucca flowering and compared the number of moths caught in traps between dark-controlled and light-treated trials. Adult moth abundance was much higher when light was present, which suggests that ALAN may alter this diurnal moth's activity patterns to expand their temporal niche into the night. To evaluate ALAN effects on yucca fruit set and moth larva recruitment, we measured skyglow exposure above yucca plants and direct light intensity from a second set of light towers. Both larva and fruit recruitment increased with skyglow, and fruit set also increased with direct lighting, but the relationship was weaker. Contrarily, larva recruitment did not change when exposed to a gradient of direct light, which may instead reflect effects of ALAN on moth physiology, such as disrupted female oviposition, or misdirecting behaviors essential to oviposition activity. Our results suggest that ALAN can positively influence the fitness of both plants and moths in this tightly co-evolved mutualism, but the benefits to each species may depend on whether night lighting is direct or indirect. Whether such effects and mechanisms could relate to susceptibility to the presence of ALAN on this or other plant-pollinator relationships will remain an important focus of future research.

What to grow: An annotated list of native larval food plants of butterflies and diurnal moths in the Darwin region, Northern Territory

What to grow: An annotated list of native larval food plants of butterflies and diurnal moths in the Darwin region, Northern Territory

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae).

Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), nine ionotropic receptors (IRs), eight gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR co-receptor (ORco), ionotropic receptors co-receptor, CO2 receptors, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, six ORs, five IRs and two GRs displayed female-biased expression, while two ORs displayed male-biased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

Sexual communication in diurnal moths: behaviors and mechanisms

Butterflies and moths have substantially different daily activities; butterflies are diurnal, while moths are largely nocturnal or crepuscular. Diurnal moths are subject to different evolutionary pressures that affect several aspects of their behavior and physiology, particularly sexual communication. In this review, species of diurnal moths and the behaviors and mechanisms of their sexual communication are summarized. Diurnal moths are day–flying insects whose partner–finding strategies include visual, olfactory and auditory signals. Males of diurnal Lepidoptera find mating partners using olfactory cues (e.g., sex pheromones) over relatively long distances, or visual (e.g., compound eyes) and/or auditory cues (e.g., ears) over short distances, or even act in combination with the three types of signals. Pheromone–binding proteins and histamine and visual genes play important roles during the signal conduction of sexual communication in diurnal moths. However, the regulatory mechanisms of acoustic communication in day–flying moths are unclear. Understanding this information may help us to explore the evolution of sexual communication in Lepidoptera and to improve biotechnological control strategies against harmful day–flying moths.

Antennal transcriptome analysis and expression profiles of putative chemosensory soluble proteins in Histia rhodope Cramer (Lepidoptera: Zygaenidae)

Histia rhodope Cramer (Lepidoptera: Zygaenidae) is one of the most destructive defoliators of landscape tree Bischofia polycarpa (Levl.) Airy Shaw in China stretching to other Southeast Asia regions. Olfactory genes, encoding proteins such as odorant carrier proteins believed to initiate olfactory signal transduction in insects, have been acknowledged to be novel targets for pest control. In this study, we established antennal transcriptome of H. rhodope and ultimately identified 19 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs) and 4 Niemann-Pick type C2 proteins (NPC2s). The 19 OBPs, 6 CSPs and 4 NPC2s were assessed to validate the differential expressions between sexes, and between olfactory and non-olfactory tissues. 8 OBPs and 2 CSPs exhibited male-biased antennae expression, while 6 OBPs, 2 CSPs and HrhoNPC2a exhibited female-biased antennae expression. Moreover, 17 OBPs, 4 CSPs and 2 NPC2s were predominantly expressed in the antennae compared with non-olfactory tissues. HrhoOBP1 and HrhoOBP8 were predominantly expressed in the antennae and heads, HrhoCSP8 and HrhoCSP14 were highly expressed in abdomens and legs, HrhoNPC2c was highly expressed in abdomens, while HrhoNPC2d was expressed in all tissues. Phylogenetic analysis revealed that most H. rhodope proteins were closely related to proteins from other moths. Moreover, compared with other nocturnal moths, acting as a diurnal moth, we found that H. rhodope may have lost a PBP gene. Our results provide important molecular information for further studies on olfactory mechanisms of H. rhodope.

M.F. Braby, D.C. Franklin, D.E. Bisa, M.R. Williams, A.A.E. Williams, C.L. Bishop and R.A.M Coppen: Atlas of butterflies and diurnal moths in the monsoon tropics of northern Australia

M.F. Braby, D.C. Franklin, D.E. Bisa, M.R. Williams, A.A.E. Williams, C.L. Bishop and R.A.M Coppen: Atlas of butterflies and diurnal moths in the monsoon tropics of northern Australia

Potential migratory routes of Urania boisduvalii (Lepidoptera: Uraniidae) among host plant populations

AbstractAimMigratory species depend on various habitats and resources along their migration routes. Characteristics such as dependence on distinct habitats and the presence of multiple threats along their migratory routes make these species vulnerable, and gaps in knowledge about their ecology and migration processes make them difficult to conserve. Urania boisduvalii is a diurnal moth endemic to Cuba that feeds on plants of Omphalea spp. during its larval phases. These plants produce secondary metabolites as a defence against the moth's larvae, which then are forced to migrate. Although some ecological aspects of Urania boisduvalii are known, its migration routes remain largely unknown. This research proposes potential migratory routes of Urania boisduvalii among populations of its host plant.LocationCuba.MethodsWe developed ecological niche models of the moth and its hosts based on environmental, anthropic, biotic and biogeographic factors to obtain potential distributional areas that include zones where positive interactions are found but exclude those where negative factors are present. These areas were overlapped to hypothesize potential breeding areas for the moths. Potential migratory corridors were proposed based on environmental connectivity.ResultsThe moth and its hosts have broad potential distributions; however, limiting factors have substantially reduced these areas, especially for plants. The potential migratory routes of Urania boisduvalii are complex and mostly involve the western and eastern regions of Cuba. Most records outside potential breeding areas were close to these migratory corridors.Main conclusionsWe offer initial hypotheses of the migratory routes of U. boisduvalii, which may be useful to guiding monitoring projects that can provide more definitive views of the seasonal distribution of this species across the Cuban archipelago.