PurposeTo determine the prevalence of malocclusion in Portuguese preschool children, to characterise occlusion in the deciduous dentition, and to study the relationship between malocclusion, sex, and age.MethodsThis cross-sectional study included 3–6 years old children, from 25 randomly selected kindergartens from the Lisbon district. Data were collected through an oral examination. Malocclusion was defined as the presence of any type of occlusion anomaly (anterior crossbite, edge-to-edge, increased overjet, open bite, deep overbite, posterior crossbite, scissor bite), spacing anomaly (crowding), or dentition anomaly (supernumerary teeth, agenesis, malformations). Canine class, terminal plane of the deciduous second molars and type of Baume arch were also recorded. Statistical analysis included descriptive and chi-squared test (α = 0.05).ResultsThe sample included 426 children with a global prevalence of malocclusion of 60.6%. Occlusion anomalies were the most prevalent (59.2%), the most frequent being deep overbite (27%), increased overjet (22.1%) and posterior crossbite (14.1%). Crowding had a prevalence of 1.6% and agenesis of 1.4%. Class I canine (57.3%), straight second molar terminal plane (60.1%) and type I Baume arch (53.3%) were the most common. The mean overjet was 2.6 mm (sd = 2.1) and the mean overbite was 2.2 mm (sd = 2.1). Age was associated with the presence of occlusion anomalies (p = 0.03), and increased overjet prevalence was found to be higher in girls (p = 0.03).ConclusionsThe prevalence of malocclusion was high, with occlusion anomalies being the most prevalent. It is important to identify modifiable risk factors associated with malocclusion to prevent this condition in both the deciduous and permanent dentitions.