The effectiveness of the teaching profession is largely dependent on their natural teaching talent. Keeping in view this utmost importance of teachers’ talents for teaching the study was carried out with the purpose of investigating the teaching talent of school teachers teaching in urban and rural schools of district Multan. By using simple random sampling technique, total 304 teachers including 161 urban and 143 rural teachers were considered as sample of the study. To measure teachers’ natural talent for teaching, a standardized Teaching Aptitude Test was thought appropriate to be used to collect data. Teachers in urban and rural schools were compared for their teaching talent by using Z-test. Data examination showed that there is significant difference between the teaching aptitude of urban and rural school teachers. The study recommends to recruit teachers on the basis of measuring their teaching talents through any standardized aptitude test and further practical test in form of demonstration