This study scrutinizes the two-dimensional concentration distribution for a solute cloud containing a micropolar fluid in a rectangular microchannel under the influence of an applied electric field. The concentration distribution is obtained up to second order approximation using Mei's homogenization method. Analytical formulas are derived for dispersion coefficient, mean and two-dimensional concentration distributions. This study also includes the analytical expressions for electric potential, velocity, and microrotation profiles. This study discusses the impact of coupling number, couple stress parameter, electric double layer thickness, and Péclet number on solute concentration distribution. The results of fluid velocity and dispersion coefficient are validated with available works in the literature. The non-Newtonian parameter and electric double layer thickness are shown to have a significant impact on dispersion. Our study reveals that concentration distribution rises but spreading of solute reduces when the coupling number increases. This is also true when the Debye length decreases. It is also obtained that the solute spreads more in the Newtonian fluid case compared to the micropolar fluid case. Finally, coupling number and electric double layer thickness show a symmetric pattern to the indicator function for the transverse concentration variation rate. The findings of this work have broad implications in deoxyribonucleic acid analysis, chemical mixing, and separation.