Distribution Of Grain Density Research Articles

Development of a small scale method to determine volume and density of individual barley kernels, and the relationship between grain density and endosperm hardness

This study reports the measurement of grain density and grain volume of barley grains using a gas displacement technique. The densities of single kernels were determined and were highly correlated with measurements of larger quantities of kernels (10, 25 or 800). Grain weight and volume were significantly correlated when measuring 800 kernels (0.99, P < 0.001), 25 kernels (0.99, P < 0.001), 10 kernels (0.98, P < 0.001), and a single kernel (0.97, P < 0.001), and the standard deviations were low for the five replicate measurements. The relationship between grain density and endosperm hardness was significantly correlated (0.57, P < 0.001), as was the relationship between grain density and test weight (0.70, P < 0.001). Grain density was negatively correlated to moisture content (−0.65, P < 0.001), but not protein content. Endosperm hardness was positively correlated to protein content (0.45, P < 0.001), but not moisture content. Although grain density and endosperm hardness are interdependent physico-chemical traits, different grain components appear to interact independently. Gas displacement technique could be applied to determine the distribution of grain density within a sample. Therefore, this technique will assist breeding programs to identify genomic regions associated with these physico-chemical traits, and produce superior malting and feed quality through uniform varieties with inherently denser, plumper grain.

Microstructure Simulation of Die Casting Magnesium Alloy

A cellular automaton (CA) based model for two-dimensional simulation of the grain morphology of high pressure die casting magnesium alloy was developed. The heterogeneous nucleation, the solute redistribution both in liquid and solid, the interface curvature and the growth anisotropy during solidification were also considered in the model. By fitting the curve of grain density distribution, parameters of continuous nucleation equation based on Gaussian distribution were calculated. The microstructure simulation of step-shape die castings of AM50 magnesium alloy was studied. The metallographic microstructure of the castings at the surface and center of three steps with different initial die temperature was investigated. The simulation results were compared with the experimental results and they were in good agreement on average grain size.

The steady-state concentration gradient for 3H-noradrenaline generated by uptake1 in the extracellular space of the rat vas deferens incubated with this amine

The rat vas deferens was incubated with 0.2 mumol/l 3H-noradrenaline for 60 min, washed out with amine-free solution for 100 min and then prepared for autoradiography (same tissues as presented by Azevedo et al. (1990) Naunyn-Schmiedeberg's Arch Pharmacol 342:245-248). The autoradiographic images were then digitized, and grain density was determined as a function of the distance from the surface of the tissue. When neither monoamine oxidase nor vesicular uptake was impaired, i.e. under control conditions, grain density declined monophasically exponentially towards the centre of the tissue. Tis decline amounted to 0.017 micron-1 or 0.124 varicosity-1, since the average distance between varicosities was calculated to be 7.4 microns. After inhibition of monoamine oxidase and vesicular uptake the rate constant was significantly reduced, and the grain density in close proximity of the surface of the tissue was also reduced. It is proposed that the distribution of grain density observed in controls reflects the steady-state concentration gradient that is generated by uptake1 during the incubation with 3H-noradrenaline. During spontaneous efflux of 3H-noradrenaline one has to distinguish between "re-uptake of the 3H-amine into the leaking varicosity" and "uptake en passant" (during diffusion through the extracellular space). On the basis of the present results, the extent of "uptake en passant" was calculated (with a computer-assisted model) for the spontaneous efflux of heterogeneously distributed 3H-noradrenaline (after wash-out). "Uptake en passant" into varicosities located between the source of efflux and the medium amounted to about 55% of the net leakage of 3H-noradrenaline from all varicosities.(ABSTRACT TRUNCATED AT 250 WORDS)

Restitution of function and collateral sprouting in the cat spinal cord: The deafferented animal

AbstractUnilateral hindlimb deafferentation is followed initially by generalized loss of all reflex and useful function. As useful movement returns, certain descending reflexes recover and become hyperactive: crossed reflexes do not. Recovery of movement and reflex hyperactivity are then abolished by subsequent destruction of the ipsilateral descending systems. Although there is no sprouting from contralateral dorsal roots, bilateral degeneration of descending systems is asymmetrical, being greater in density and distribution on the previously deafferented side. Collateral sprouting to certain spinal laminae apparently took place during the recovery period from the same source which mediated reflex hyperactivity; the ipsilateral descending systems. A substantial descending projection to Clarke's nucleus was found only on the experimental side, however, suggesting that a central feedback system replaced the abolished peripheral feedback. If functional, this might subserve recovery of the ability to maintain a limb position.Chronically deafferented cats which are then transected show, for the first time, hyperreflexia of crossed responses, which develops gradually during the first week post‐transection. It may be related to sprouting of contralateral dorsal roots for contralateral ganglion injection with 3H‐proline gives a larger than normal ipsilateral distribution of grain density over the commissural nuclei.It is suggested that recovery and hyperactivity of reflex responses underlie the recovery of useful movement and that reflex recovery may be related to collateral sprouting. It is also suggested that both the reflex and the anatomical response to a given lesion is restricted to only one of the remaining systems and is therefore a specific rather than a generalized response.