Additive manufacturing (AM) offers distinct advantages in terms of complexity, reduced material waste, and shorter production times. However, the microstructures of AM alloys differ significantly from conventionally manufactured ones, and their impact on grain behavior remains uncertain. This study employs neutron diffraction, coupled with Crystal Plasticity Finite Element (CPFE) modeling, to investigate microstructural effects on cyclic behavior in 316L LPBF alloys. Neutron diffraction provides high-resolution strain and stress data at the polycrystalline level during loading, offering valuable insights into grain behavior. The CPFE model is initially validated at the grain scale using neutron diffraction, demonstrating its ability to predict local mechanical behavior in additively manufactured materials. This methodology holds promise for broader applications in various AM alloys, particularly when macroscopic identification of mechanical behavior is insufficient. Furthermore, the comparison between neutron diffraction and CPFE predictions allows to identify the influence of dendrites and dislocation substructures on the mechanical behavior at the grain scale. Notably, the micromechanical anisotropy resulting from dislocation organization along dendritic structures with specific orientations is highlighted. This study shows that despite the observed anisotropy, experimental measurement of intragranular strain distribution are correctly captured with a quantification of the deviation observed on some specific orientations due to the above cited microstructure sub-structures.