Distillers Dried Grains With Solubles Diets Research Articles

Effect of feeding a dried distillers' grains with solubles diet on the metabolism of the intestinal wall in Guanling crossbred cattle: a preliminary assessment.

Dried distillers' grains with solubles (DDGS)-based diets are nutritious and can improve the inflammations and intestinal immunity in livestock. However, there is limited research examining the effect of feeding DDGS-based diets on changes in intestinal metabolites and related pathways in livestock. In this study, six Guanling crossbred cattle (Guizhou Guanling Yellow cattle × Simmental cattle) were selected and divided into a basal diet (BD) group and an experimental group fed with DDGS replacing 25% of the daily ration concentrates (DDGS) (n=3), respectively. Fresh jejunum (J), ileum (I) and cecum (C) tissues were collected for metabolomic analysis. Differential metabolites and metabolic pathways were explored by means of univariate and multivariate statistical analysis. In comparison to the J-BD group, 123 differential metabolites (VIP > 1, p < 0.05) were identified in the J-DDGS group, which (top 20) were mainly divided into superclasses, including lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic compounds. Compared with the I-BD group, 47 differential metabolites were obtained in the I-DDGS group, which were mainly divided into superclasses, including lipids and lipid-like molecules and organic acids and derivatives. The C-DDGS vs. C-BD comparison revealed 88 differential metabolites, which were mainly divided into superclasses, including lipids and lipid-like molecules, organic oxygen compounds, and nucleosides. A total of 34 significant metabolic pathways were found (p < 0.05, -log(p) > 1.3). Among them, 3 significant pathways were significantly enriched in the J-DDGS group, 11 significant pathways were significantly enriched in the I-DDGS group, and 20 significant pathways were significantly enriched in the C-DDGS group. Importantly, primary bile acid biosynthesis, linoleic acid metabolism, and arachidonic acid metabolism correlated with intestinal inflammation and immunity by regulating gut microbiota, prostaglandin synthesis, and cell signaling. The data suggest that DDGS-fed cattle unregulated three metabolic pathways mentioned above and that a DDGS-based diet was able to maintain a balance of these three metabolic pathways, thus resulting in improvement of intestinal inflammation and enhanced immunity in cattle. In conclusion, the DDGS diet has the potential to improve intestinal inflammation and enhance the immunity of Guanling crossbred cattle by regulating the metabolic patterns of lipids and lipid-like molecules, organic acids and derivatives, and related metabolic pathways. These results allude to potential metabolic regulatory mechanisms of DDGS diets and also provide a theoretical basis for the application of DDGS in livestock feed.

Características nutricionais de grãos secos destilados com solúveis e seus efeitos no desempenho e viabilidade econômica para suínos

Abstract The chemical and nutritional characteristics of the four distillers dried grains with solubles (DDGS) were determined for dry matter (DM), mineral matter (MM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF) and gross energy analyzed (GEa), from which the values of gross energy (GE), digestible energy (DE), metabolizable energy (ME) and net energy (NE) were estimated. There were differences in the contents of EE, NDF, GEa, GE, DE, ME and NE between the lots. A total of 40 barrows with an initial weight of 72.69 ± 5.66 kg were assigned to a randomized block design, fed diets containing 0, 100, 200 and 300 g kg−1 DDGS for 28 days and were subjected to performance assessment. The inclusion of DDGS in the diets did not affect daily feed intake (DFI), daily weight gain (DWG) or feed conversion (FC) but linearly reduced the final weight. Based on the results of the performance and prices of ingredients in the local market, we calculated the costs of feed, revenue and gross operating profit (GOP). Inclusions of DDGS in pig diets reduced costs and revenues but did not affect GOP. DDGS presented chemical and nutritional variation between the lots. Levels of inclusion up to 300 g kg−1 DDGS do not affect finishing pig performance and profitability.

Evaluation of Distillers Dried Grains With Solubles in Aquafeeds – A Review

Abstract The aquafeed industry faces many challenges, including reducing the cost of feed by reducing the quantity of fish meal and soybean meal and researching to replace them with other ingredients lower in cost. Furthermore, new low-cost feeds must improve growth and feed utilization while also ensuring fish health. During the past few decades, researchers have shown one of the low-cost and with appropriate nutritional value alternative protein sources to replace fish meal (FM) or soybean meal (SBM) is dried distillers grains with solubles (DDGS). Recently, the production of grain-based ethanol as a fuel additive has been increasing as the demand for biofuel has increased, and lessening dependency on fossil fuels has resulted in a dramatic rise in the amount of grains used for ethanol. DDGS are the primary co-product of dry-grind fuel ethanol plants. DDGS has the advantages of having high protein, fat, phosphorus, yield, and low cost. These valuable particles attributed to DDGS have raised controversy amongst feed nutritionists as to how they can proficiently replace fish meal or soybean meal. It has been discovered that DDGS can be replaced with fish meal or soybean meal without affecting growth. Therefore, the present review discusses the effects of dietary DDGS application in the fish feed formulation, the best dietary presence, the possible advantages in fish health, nutrient retention, and the economic benefits of DDGS application in aquafeeds.

Impact of feeding dried distillers' grains with solubles diet on microbiome and metabolome of ruminal and cecal contents in Guanling yellow cattle.

Dried distillers' grains with solubles (DDGS) are rich in nutrients, and partially alternative feeding of DDGS effectively reduces cost of feed and improves animals' growth. We used 16S rDNA gene sequencing and LC/MS-based metabolomics to explore the effect of feeding cattle with a basal diet (BD) and a Jiang-flavor DDGS diet (replaces 25% concentrate of the diet) on microbiome and metabolome of ruminal and cecal contents in Guanling yellow cattle. The results showed that the ruminal and cecal contents shared the same dominance of Bacteroidetes, Firmicutes and Proteobacteria in two groups. The ruminal dominant genera were Prevotella_1, Rikenellaceae_RC9_gut_group, and Ruminococcaceae_UCG-010; and the cecal dominant genera were Ruminococcaceae_UCG-005, Ruminococcaceae_UCG-010, and Rikenellaceae_RC9_gut_group. Linear discriminant analysis effect size analysis (LDA > 2, P < 0.05) revealed the significantly differential bacteria enriched in the DDGS group, including Ruminococcaceae_UCG_012, Prevotellaceae_UCG_004 and Anaerococcus in the ruminal contents, which was associated with degradation of plant polysaccharides. Besides, Anaerosporobacter, Anaerovibrio, and Caproiciproducens in the cecal contents were involved in fatty acid metabolism. Compared with the BD group, 20 significantly different metabolites obtained in the ruminal contents of DDGS group were down-regulated (P < 0.05), and based on them, 4 significantly different metabolic pathways (P < 0.05) were enriched including "Linoleic acid metabolism," "Biosynthesis of unsaturated fatty acids," "Taste transduction," and "Carbohydrate digestion and absorption." There were 65 significantly different metabolites (47 were upregulated, 18 were downregulated) in the cecal contents of DDGS group when compared with the BD group, and 4 significantly different metabolic pathways (P < 0.05) were enriched including "Longevity regulating pathway," "Bile secretion," "Choline metabolism in cancer," and "HIF-1 signaling pathway." Spearman analysis revealed close negative relationships between the top 20 significantly differential metabolites and Anaerococcus in the ruminal contents. Bacteria with high relevance to cecal differential metabolites were Erysipelotrichaceae_UCG-003, Dielma, and Solobacterium that affect specific metabolic pathways in cattle. Collectively, our results suggest that feeding cattle with a DDGS diet improves the microbial structure and the metabolic patterns of lipids and carbohydrates, thus contributing to the utilization efficiency of nutrients and physical health to some extent. Our findings will provide scientific reference for the utilization of DDGS as feed in cattle industry.

Plasma Metabolomic Profiling after Feeding Dried Distiller’s Grains with Solubles in Different Cattle Breeds

Dried distiller’s grains with solubles (DDGS) are rich in nutrients and can enhance animals’ growth and immunity. However, there are few reports on the effects of a diet of DDGS on plasma metabolism and the related action pathways in domestic animals. In this study, groups of Guanling yellow cattle (GY) and Guanling crossbred cattle (GC) having a basal diet served as the control groups (GY-CG and GC-CG), and DDGS replacing 25% of the diet of GY and GC served as the replacement groups (GY-RG and GC-RG), with three cattle in each group. Plasma samples were prepared for metabolomic analysis. Based on multivariate statistical and univariate analyses, differential metabolites and metabolic pathways were explored. Twenty-nine significantly different metabolites (p &lt; 0.05) were screened in GY-RG compared with those in GY-CG and were found to be enriched in the metabolic pathways, including choline metabolism in cancer, linolenic acid metabolism, and amino acid metabolism. Nine metabolites showed significant differences (p &lt; 0.05) between GC-RG and GC-CG and were mainly distributed in the metabolic pathways of choline metabolism in cancer, glycerophospholipid metabolism, prostate cancer metabolism, and gonadotropin-releasing hormone (GnRH) secretion. These results suggest that a DDGS diet may promote healthy growth and development of experimental cattle by modulating these metabolic pathways. Our findings not only shed light on the nutritional effects of the DDGS diet and its underlying mechanisms related to metabolism but also provide scientific reference for the feed utilization of DDGS.

Performance response of increasing the standardized ileal digestible tryptophan:lysine ratio in diets containing 40% dried distiller grains with solubles.

A total of 1,170 pigs (PIC 800 × PIC, initially 38.6 kg) were used in a 98-d grow-finish study to determine the performance response of pigs fed increasing levels standardized ileal digestible (SID) Trp:Lys ratio in diets containing 40% dried distillers grains with solubles (DDGS). Five dietary treatments were fed and consisted of a corn-soybean meal (SBM) diet or diets containing 40% DDGS with SID Trp:Lys ratios of 15%, 18%, 21%, or 24%. Each treatment was replicated 9 times and pens contained 26 pigs with the equal number of gilts and barrows. Data was analyzed as a randomized complete block design with previous nursery treatment as a random blocking factor. Pair-wise comparisons were used to evaluate the impact of diets on performance and carcass traits; specifically to compare the corn-SBM dietary treatment against other dietary treatments. Single degree of freedom orthogonal polynomials were used to evaluate the dose-response of increasing the SID Trp:Lys ratio in 40% DDGS diets. Increasing the SID Trp:Lys ratio in diets containing 40% DDGS increased (linear, P ≤ 0.02) average daily gain (ADG), average daily feed intake (ADFI), final body weight (BW), hot carcass weight (HCW), carcass gain, and daily carcass gain. Increasing the SID Trp:Lys ratio in 40% DDGS diets did not impact (P ≥ 0.65) gain to feed (G:F) for the cumulative period. Pigs fed the corn-SBM diet had greater (P ≤ 0.01) ADG and a heavier (P ≤ 0.01) final BW compared to pigs fed 40% DDGS diets, regardless of the SID Trp:Lys ratio. Diets that contained 40% DDGS with a SID Trp:Lys ratio of 24% had no difference (P|t| = 0.25) in ADFI compared to the corn-SBM dietary treatment. The cumulative G:F of pigs did not differ (P = 0.32) between pigs fed corn-SBM diets and diets containing 40% DDGS regardless of the SID Trp:Lys ratio. However, pigs receiving the corn-SBM diets had heavier HCW, greater carcass yields, total carcass gain, and daily carcass gain (P < 0.01) compared to pigs fed the 40% DDGS diets regardless of SID Trp:Lys ratio. In conclusion, increasing the SID Trp:Lys ratio in 40% DDGS diets improved ADG and ADFI for the overall grow-finish period. However, when compared to a corn-SBM diet, increasing the SID Trp:Lys ratio in 40% DDGS diets did not correct the growth performance or carcass characteristics of pigs.

Comparing tryptophan:lysine ratios in dried distillers grains with solubles-based diets with and without a dried distillers grains with solubles withdrawal strategy on growth, carcass characteristics, and carcass fat iodine value of growing-finishing pigs.

A total of 6,240 finishing pigs (DNA 600 × PIC 1050; initially 22.5 ± 1.00kg), divided into 2 groups, were used in a 119 or 120 d study comparing increasing Trp:Lys ratio in diets containing dried distillers grains with solubles (DDGS) or a DDGS withdrawal strategy (removing all DDGS from the last phase before marketing) on growth performance and carcass fat iodine value (IV). Pigs were randomly allotted to 1 of 7 dietary treatments with 30 to 36 pigs per pen and 26 replications per treatment. Diets were fed in 4 phases, approximately 23 to 44, 44 to 71, 71 to 100, and 100kg to market. Diets included a control corn-soy bean meal-based diet (no DDGS) formulated to a 19% standardized ileal digestibility (SID) Trp:Lys ratio, 4 diets with 30% DDGS fed in all four phases and formulated to provide SID Trp:Lys ratios of 16, 19, 22, or 25%, and 2 DDGS withdrawal strategy diets: 19% SID Trp:Lys with 30% DDGS in phase 1 through 3 and then 0% DDGS in phase 4 with either a 19 or 25% Trp:Lys ratio. Overall, body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), and G:F increased (linear, P < 0.05) as SID Trp:Lys ratio increased in diets with 30% DDGS fed in all phases. Simultaneously, hot carcass weight (HCW; quadratic, P = 0.014), carcass yield (quadratic, P = 0.012), and backfat depth (linear, P = 0.040) increased with increasing Trp:Lys ratio. Pigs fed the 19% SID Trp:Lys ratio withdrawal strategy diet had similar ADG and ADFI as those fed the control diet, the 25% Trp:Lys withdrawal diet, or the 30% DDGS diets with 25% Trp:Lys ratio throughout the study. Pigs fed the control diet had decreased (P < 0.05) carcass fat IV compared to pigs fed DDGS diets throughout the study, with pigs fed the two DDGS withdrawal strategy diets intermediate. In summary, increasing the SID Trp:Lys ratio in diets with 30% DDGS resulted in a linear increase in ADG, ADFI, G:F, and BW but did not influence carcass fat IV, with most of the benefit observed as diets increased from 16 to 19% Trp:Lys. Removing DDGS from the diet in the last period reduced carcass fat IV and increased growth rate during the withdrawal period compared to pigs fed 30% DDGS throughout, indicating value in a withdrawal strategy.

Evaluation of DDGS as a Low-Cost Feed Ingredient for Common Carp (Cyprinus carpio Linneus) Cultivated in a Semi-Intensive System.

Distillers dried grains with solubles (DDGS), a coproduct from the ethanol production industry, is successfully used as an ingredient in feeding cattle and pigs due to its relatively high protein and nutrient content and low price compared to cereals. The aim of this study was to establish the optimal DDGS concentration that can be included in the diet of common carp. A seven-week experiment was performed on common carp with an initial weight of 86 g feed with three experimental diets D0 (DDGS 0%), D1 (DDGS 25%) and D2 (DDGS 35%). The chemical composition of DDGS analyzed by Fourier Transform Near-Infrared (FT-NIR) spectroscopy showed a protein content of 27.56% and oil at 6.75%. Diets with DDGS did not produce significant changes in growth parameters, flesh quality, and blood biochemical profile. Regarding the oxidative status in the muscle tissue, D1 and D2 significantly reduced, in a dose-dependent manner, the specific activity of SOD and GSH, while CAT and GPX were left unaffected. In the liver tissue, CAT, GSH, MDA and carbonylated proteins were reduced in the DDGS diets. The microbiological analysis of the intestinal contents revealed a variation in microbial density depending on the diet used. The total number of aerobic germs was between 224.2 × 104 and 69.84 × 106 (D2 > D1 > D0) and the total number of anaerobic germs was between 15.2 × 102 and 28.2 × 102 (D2 > D0 > D1).

Adding dried distillers grains with solubles influences the rumen microbiome of meat goats fed lespedeza or alfalfa-based diets

We evaluated the effect of replacement of corn and soybean meal (SBM) with dried distillers grains with solubles (DDGS) in sericea lespedeza (SL; Lespedeza cuneata) and alfalfa (Medicago sativa)-based diets on ruminal fermentation and bacterial community of growing meat goats. Thirty-six male Spanish growing meat goats with an average body weight of 24 ± 1.8 kg were stratified by body weight and assigned to four isonitrogenous (crude protein = 17.1 ± 0.1%) and isocaloric (3.2 ± 0.09 Mcal/ kg DM) diets for 60 d in a randomized design with a 2 × 2 factorial arrangement. The dietary treatments were (1) alfalfa-based diet (ALC), (2) alfalfa + DDGS (ALD; replacement of corn and SBM with DDGS), (3) SL-based diet (SLC) and (4) SL + DDGS (SLD; replacement of corn and SBM with DDGS). The goats were slaughtered, and rumen contents were immediately sampled for 16 S rRNA gene sequencing and rumen fermentation analysis. The analysis of Similarity (ANOSIM) and linear discriminant analysis (LDA) effect size (LEfSe) were respectively used to detect microbial community shift and differentially abundant taxa among treatments. Addition of DDGS increased (P < 0.05) rumen acetate concentrations in both alfalfa and lespedeza-based diets. However, dietary DDGS reduced (P < 0.05) butyrate and ammonia nitrogen concentrations, compared to alfalfa and SL-based diets. The ANOSIM results revealed a shift in the bacterial community between ALC and ALD (R = 0.251; P = 0.01) and between SLC and SLD (R = 0.289; P = 0.001). The LEfSe analysis revealed that the relative abundance of 7 taxa, including Ruminococcaceae UCG 002, Succinivibrionaceae UCG 002, Prevotellaceae YAB2003, and Lachnospiraceae UCG 010 were reduced (LDA > 2; P < 0.05) while 38 taxa were enriched (LDA > 2; P < 0.05) in ALD compared to ALC. Similarly, the relative abundance of 31 taxa, including Ruminococcus YE281, Succinivibrio, and Lachnospiraceae ND3007 were reduced, while 35 taxa were enriched (LDA > 2; P < 0.05) in SLD compared to SLC. In conclusion, this study showed that regardless of the forage type, inclusion of DDGS in diet modulates the relative population of both fibrolytic and butyrate-producing bacteria in meat goats.

Corn distiller's dried grains with solubles as an alternative ingredient to corn and soybean meal in Pekin duck diets based on its predicted AME and the evaluated standardized ileal digestibility of amino acids

The study aimed to investigate the effects of dietary distillers dried grains with solubles (DDGS) levels on growth performance, carcass characteristic, serum biochemical indexes, meat physical and chemical quality, nutrient utilization, and standardized ileal digestibility of amino acids (SIDAA) in Pekin ducks aged 11 to 42 d based on the evaluation of its SIDAA. A total of 560 eleven-day-old Cherry Valley ducks were randomly allotted to 5 treatments with 7 replicate pens per treatment and 16 ducks per pen based on the average body weight. Six isonitrogenous and isocaloric experimental diets were formulated on a digestible amino acid basis to produce diets containing 0, 5, 10, 15, and 20% DDGS, respectively. With increasing of dietary DDGS levels, a linear and quadratic reduction (P < 0.05) was observed in the body weight (BW) at d 42, average day gain (ADG) and average day feed intake (ADFI) from d 11 to 42, breast meat yield, the moisture and protein content in the breast meat, and dietary DM and EE utilization. Moreover, a linear and quadratic increase (P < 0.05) was observed in the b* value of the breast meat and serum total cholesterol and triglyceride concentrations. Compared with the control group, the group with 10% inclusion of DDGS exhibited no adverse effect on growth performance, carcass characteristics, serum biochemical indexes, meat physical and chemical quality, nutrient utilization, and the SIDAA of the diets (P > 0.05). These results suggested that 10% of corn DDGS can function as an alternative ingredient to corn and soybean meal, and the optimal levels of DDGS in the diets of ducks aged from 11 to 42 d depends more on its quality.

The Interaction of Dried Distillers Grains With Solubles (DDGS) Type and Level on Growth Performance, Health, Texture, and Muscle-Related Gene Expression in Grass Carp (Ctenopharyngodon idellus)

The aim of this study was to estimate the possible synergetic effects of the two levels of dietary dried distillers grains with solubles (DDGS) from different sources (US-imported and native) on the growth, health status, muscle texture, and muscle growth-related gene expression of juvenile grass carp. Four treatments of fish were fed with 4 isonitrogenous diets, namely, native DDGS20, native DDGS30, US-imported DDGS20, and US-imported DDGS30 for 60 days. The US-imported DDGS30 group showed the better growth and feed efficiency. Additionally, we observed a significant increase in hepatopancreatic total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in native DDGS groups. Moreover, raw muscle collagen increases considerably in the US-imported DDGS30 compared with the native DDGS30 group. In comparison with the native DDGS groups, the US-imported DDGS groups showed significantly decrease in all textural properties and fiber density, while increased fiber diameter. Dietary native DDGS inclusion significantly showed the upregulation of myog, myhc, and fgf6a expression in muscle, while the downregulation of the expression of myod and myf5. Overall, US-imported DDGS30 had a beneficial influence on growth via regulating genes involved in myogenesis and hypertrophy, the formation of collagen, but had negative impacts on antioxidant capacity and cooked muscle texture.

168 Evaluation of Increasing Dietary Threonine to Lysine Ratio in Corn-Soybean Meal Diets with and Without Dried Distillers Grains with Solubles (DDGS) on Growth Performance of Grow-Finish Pigs

Abstract A total of 2,160 pigs (PIC 337×1050; initial BW 35.1 kg) were used in a 112-d growth trial to evaluate the effects of normal or high SID Thr:Lys ratio in diets with and without DDGS on growth performance. Pigs were assigned to pens (27 pigs per pen) in a randomized complete block design by BW with 20 replications per treatment. Pens of pigs were allotted to 1 of 4 dietary treatments arranged in a 2×2 factorial with main effects of dietary Thr level (Normal vs High) and DDGS (with or without). Treatment diets were formulated in 4 phases from 35 to 57, 57 to 82, 82 to 105, and 105 to 136 kg BW. Diets with high DDGS were formulated to include 40% DDGS in phase 1 and 2, 30% in phase 3, and 15% in phase 4. Normal Thr diets were formulated to contain 61, 62, 63, and 65% SID Thr:Lys ratios for the 4 dietary phases, respectively. High Thr diets had SID Thr:Lys ratios of 67, 68, 69 and 72%, respectively. There were no (P&amp;gt;0.10) DDGS×Thr interactions. For the overall period (d 0 to 112), pigs fed diets without DDGS had increased (P &amp;lt; 0.001) ADG and BW, and reduced (P &amp;lt; 0.001) ADFI leading to improved (P &amp;lt; 0.001) G:F. There was no evidence for difference (P &amp;gt; 0.10) between diets with normal or high SID Thr:Lys ratio regardless of DDGS inclusion. In summary, the addition of high levels of DDGS reduced ADG and increased ADFI, which resulted in poorer G:F and lighter final BW, regardless of the dietary SID Thr:Lys ratio. These results indicate that addition of an insoluble fiber source, such as corn DDGS, does not increase the Thr:Lys requirement of finishing pigs.

Evaluation of increasing digestible threonine to lysine ratio in corn-soybean meal diets without and with distillers dried grains with solubles on growth performance of growing-finishing pigs.

Corn distillers dried grains with solubles (DDGS) is commonly available and often can lower diet cost for swine diets. This corn co-product is recognized to be a source of dietary insoluble fiber, which can not only increase the villous length of the gut mucosa but also increase intestinal production of mucin. Mucin structure, functions, and synthesis are correlated to Thr intake; consequently, the dietary Thr level may need to be increased when feeding an insoluble fiber source such as corn-DDGS. Thus, the objective of this study was to evaluate if feeding standardized ileal digestible (SID) Thr:Lys ratio at or above the estimated requirement in diets without and with DDGS would influence growth performance in finishing pigs. A total of 2,160 pigs (PIC 337 × 1,050; initially 35.1 ± 0.5 kg) were used in a 112-d growth trial. Pigs were randomly assigned to pens (27 pigs per pen) in a randomized complete block design by body weight (BW) with 20 replications per treatment. Pens of pigs were allotted to one of four dietary treatments that were arranged in a 2 × 2 factorial with main effects of dietary Thr level (normal vs. high) and DDGS (without or with). Treatment diets were formulated in four phases from 34 to 57, 57 to 79, 79 to 104, and 104 to 130 kg BW. Diets with high DDGS were formulated to include 40% DDGS in phases 1 and 2, 30% in phase 3, and 15% in phase 4. The normal Thr diets were formulated to contain 61%, 62%, 63%, and 65% SID Thr:Lys ratios for the four dietary phases, respectively. High Thr diets had SID Thr:Lys ratios of 67%, 68%, 69%, and 72%, respectively. There were no interactions (P > 0.10) observed in any phase or overall between Thr level and added DDGS. For the overall period (day 0 to 112), pigs fed diets without DDGS had increased (P < 0.001) average daily gain (ADG) but reduced (P < 0.001) average daily feed intake (ADFI), leading to increased (P < 0.001) feed efficiency (gain-to-feed ratio [G:F]). There was no evidence for difference (P > 0.10) between pigs fed diets formulated at normal or high SID Thr:Lys ratio. In summary, feeding high levels of DDGS decreased ADG and increased ADFI, which resulted in decreased G:F and lower final BW, regardless of the dietary SID Thr:Lys ratio level. In the current study, increasing the level of digestible Thr in a diet that contained a highly insoluble fiber source did not increase the growth performance of grow-finish pigs.

The effects of various levels of distillers dried grains with solubles (DDGS) and a dacitic (rhyolitic) tuff breccia on pellet production rate and durability

Distillers dried grain with solubles (DDGS) has been associated with a reduction in pellet production efficiency and quality in animal diets, whereas some minerals may improve pellet efficiency by scouring the surface of the pelleting die holes, reducing friction. The objective of this study was to understand the relationship between DDGS and a dacitic tuff breccia (DTB) based on pellet production rate and pellet durability index (PDI). The experiment was conducted in a complete block design, with a factorial arrangement 5 × 2, and repeated on 2 d. DDGS levels (0, 4, 8, 12, and 16%) and DTB (0 and 0.25%) were the main effects. Interactions between DDGS and DTB were observed for production rate throughout the study, however, the effects were not consistent between days. DTB inclusion improved the production rate of diets containing 12% DDGS on day 1 ( P = 0.010), whereas, on day 2, this positive effect was observed in DTB diets with 4 and 8% DDGS compared to their respective controls ( P = 0.001). DTB inclusion had a positive main effect on PDI on day 1 ( P > 0.015), however, this result was not observed on day 2 ( P > 0.314). Increasing levels of DDGS in the diet had a positive influence on PDI throughout the experiment ( P < 0.001). The results showed the potential of DTB in improving pellet production rate when dietary DDGS inclusion ranged from 4% to 12%.

Effects of feeding high oleic soybean oil to growing-finishing pigs on growth performance and carcass characteristics.

Feeding growing-finishing pigs supplemental fat is a common practice in the swine industry and can result in improved feed efficiency and reduced feed intake; however, dietary lipids also play a key role in determining pork composition. The objectives of the current study were to evaluate the effects of feeding graded levels of high oleic soybean oil (HOSO) on growth performance and carcass characteristics. A total of 288 pigs raised in two separate blocks (144 pigs each) were assigned to one of four diets containing either 25% dried distiller's grains with solubles (DDGS), 2% high oleic soybean oil (HOSO2), 4% high oleic soybean oil (HOSO4), or 6% high oleic soybean oil (HOSO6). Pigs were housed 4 per pen and fed for 98 d using a 3-phase feeding system. Pigs were individually weighed and feed intake was recorded throughout the trial to calculate average daily feed intake (ADFI) and gain to feed ratio (G:F). A total of 144 pigs were transported to the University of Illinois Meat Science Laboratory and fabricated into primal and subprimal cuts to calculate carcass cutting yields. Differences in growth performance were observed, with pigs fed the DDGS treatment exhibiting greater (P ≤ 0.01) overall ADFI consuming 0.21, 0.18, and 0.28kg/d more than HOSO2, HOSO4, and HOSO6 diets, respectively. Pigs fed the HOSO6 diet had greater (P ≤ 0.03) overall G:F than pigs fed DDGS and HOSO2 diets but did not differ (P = 0.12) from pigs fed HOSO4. Furthermore, differences in carcass traits were observed. Hot carcass weight was increased (P ≤ 0.03) in pigs fed the HOSO6 diet compared with pigs fed the DDGS and HOSO2 diets, while pigs fed HOSO4 did not differ (P > 0.05) from either extreme. Additionally, pigs fed HOSO4 and HOSO6 produced fatter (P ≤ 0.01) carcasses with reduced (P ≤ 0.01) standardized fat-free lean. Minimal differences were observed in primal weights expressed as a percentage of chilled side including bone-in Boston butt, trimmed loin, and trimmed ham with primal weights decreasing with increasing inclusion of dietary HOSO. Overall, pigs fed HOSO2 had reduced ADFI with similar backfat thickness and standardized fat-free lean compared with pigs fed the DDGS treatment. However, pigs fed HOSO 4% and 6% not only had improvements in ADFI and G:F but also had increased backfat thickness, which resulted in reductions in standardized fat-free lean and primal weights expressed as a percentage of chilled side weight.

Nutrient digestibility of heat- or heat plus citric acid-pretreated dried distillers grains with solubles for pigs

A study was conducted to determine the effects of pretreating whole stillage (WS) with heat or heat plus diluted citric acid on nutrient digestibility of the resulting dried distillers grains with solubles (DDGS) for growing pigs. The WS was untreated or pretreated with heat (160 °C at 70 psi for 20 min) alone (heat) or with the heat plus citric acid (12 g/L; heat+CA) at 70 psi for 20 min. Untreated and pretreated WS were paddle-dried before their inclusion in diets. Five diets were fed. The diets were cornstarch-based containing DDGS, untreated WS, heat-pretreated WS, or heat+CA -pretreated WS as the sole source of protein; and N-free diet, which was included for estimation of basal ileal endogenous losses of AA. The DDGS diet was included for comparison. The 5 diets were fed to 10 ileal-cannulated barrows (57 ± 1.53 kg body weight) in a replicated 5 × 5 Latin square to give 10 replicates/diet. On dry matter basis, DDGS contained 307 g/kg crude protein (CP), 37.1 g/kg starch, 31.1 g/kg ether extract (EE), and 342 g/kg neutral detergent fiber; whereas untreated WS contained 369.4 g/kg crude protein, 45.1 g/kg starch, 90.4 g/kg EE, and 364.6 g/kg neutral detergent fiber. Pretreatment of WS with heat or CA improved (P < 0.05) coefficient of apparent ileal digestibility (CAID) of gross energy (GE) in diet from 0.742 to 0.823 or to 0.797, respectively; and CAID of CP in diet from 0.782 to 0.847 or to 0.820, respectively, respectively. Pretreatment of WS with heat or heat+CA did not affect coefficient of apparent total tract digestibility (CATTD) of GE in diet. The untreated WS diet had lower (P < 0.05) CAID and CATTD of GE by 4% and 2% compared to DDGS, respectively. Pretreatment of WS with heat or heat+CA increased (P < 0.05) DE value of the WS. Pretreatment of WS with heat or heat+CA reduced (P < 0.05) CAID of all indispensable amino acids (except threonine) by at least 2.91%. In conclusion, pretreatment of WS with heat or heat+CA improved energy digestibility, but reduced amino acid digestibility. Thus, pretreatment and drying of WS at conditions employed in the current study can improve energy value, but reduce amino acid availability of the resulting DDGS for pigs.

Replacing Soybean Meal with Distillers Dried Grains with Solubles plus Rumen-Protected Lysine and Methionine: Effects on Growth Performance, Nutrients Digestion, Rumen Fermentation, and Serum Parameters in Hu Sheep.

Simple SummaryImproving the economic benefits and precise nutrient supply are hotspots of the sheep breeding industry. Evaluation of the production performance, the rumen fermentation, and blood metabolism indexes found that replacement of soybean meal with distillers dried grains with solubles in a diet with adequate metabolizable protein and amino acids (lysine and methionine) could maintain the normal growth performance of Hu sheep. The comprehensive evaluation results provide a reference for reducing production costs, improving production efficiency, and decreasing the nitrogen excretion of the sheep breeding industry. Besides, the study will help in the development of low-protein diets with amino acid balance for sheep.(1) Background: we investigated the influence of dietary soybean meal (SBM) replaced with distillers dried grains with solubles (DDGS) plus rumen-protected (RP) lysine and methionine on the growth performance, nutrients digestion, rumen fermentation, and serum parameters of Hu sheep. (2) Methods: ninety Hu sheep were allocated to five groups: the control group (CON) which received the SBM diet, the DDGS group (NSM), the DDGS diet with RP lysine group (DRPL), the DDGS diet with RP methionine group (DRPM), and the DDGS diet with a mixture of RP lysine and methionine group (DRPLM). (3) Results: Final BW and carcass weight of the DRPLM and CON groups were greater (p ≤ 0.05) compared to NSM, DRPL, and DRPM groups. The DRPLM group tended to increase the dry matter intake (DMI, p = 0.06), average daily gain (ADG, p = 0.06), dressing percentage (p = 0.07), and tail fat weight (p = 0.09). The DRPLM group had increased (p ≤ 0.05) apparent digestibility and had altered ruminal fermentation characteristics. (4) Conclusions: replacement of SBM with DDGS in a diet with adequate metabolizable protein and by-pass amino acids (lysine and methionine) could maintain the growth performance of Hu sheep.

Effects of replacing fishmeal with dietary dried distillers grains with solubles on growth, serum biochemical indices, antioxidative functions, and disease resistance for Litopenaeus vannamei juveniles

This research was conducted to assess the effects of replacing fishmeal (FM) with dried distillers grains with solubles (DDGS) at various levels (D0 (0 %), D2 (2 %), D4 (4 %), D6 (6 %), D8 (8 %), D12 (12 %), and D16 (16 %)) on the growth, proximate body composition, serum biochemical indices, antioxidant activities, histology, and disease resistance in juvenile Pacific whiteleg shrimp. Forty shrimp per tank were stocked in seven different tanks with three replicates of each treatment group with an initial weight of 0.23 ± 0.00 g. Their respective experimental diets were used to feed shrimp for 56 days. The results showed an enhancement in shrimp fed dietary DDGS (D8) diets on the growth performance, including final weight, weight gain, acid phosphatase, alkaline phosphatase, glucose, and total antioxidant capacity in the serum (P < 0.05). Decreased FCR and High-Density Lipoprotein Cholesterol were observed in shrimp fed DDGS (D8) diets in serum compared to D0. No severe changes and significant differences in villus height and intestinal wall thickness were observed in the shrimp hepatopancreas and mid-gut among treatment groups. Furthermore, disease resistance increased in shrimp fed the DDGS diet, with D8 obtaining the lowest mortality rate, 50.9 %, compared to the control group (D0), 68.3 %. In summary, regarding all the factors, replacing FM with DDGS at D8 (8 %) in the shrimp diet could modulate and cater to overall growth performance, health and immunity, histology and disease resistance in shrimp.

169 The Effect of Standardized Ileal Digestible isoleucine:lysine in Diets Containing 20% Dried Distillers Grains with Solubles on Finishing Pig Performance and Carcass Characteristics

Abstract Previous research suggests an increase of the isoleucine (Ile) requirement for late finishing pigs. Diets containing dried distillers grains with solubles (DDGS) provide excess leucine which can lead to changes in available Ile due to the antagonistic relationship of branched chain amino acids. Therefore, the Ile requirement in finishing diets containing DDGS deserve to be re-evaluated. A finishing study was conducted to determine the standardized ileal digestible (SID) Ile:Lysine (Lys) requirement in diets containing 20% DDGS. Pigs (n = 2,268; 82.3 ± 0.39 kgs) were used in a 56-d trial (14 replicates per treatment). Pens were assigned to one of six dietary treatments balancing for previous treatment. Treatments consisted of a corn-soybean meal diet (CS) or diets containing 20% DDGS with a SID Ile:lys ratio of 55, 60, 65, 70, or 75%. Data was analyzed as a randomized complete block, pair-wise comparisons and single degree of freedom orthogonal polynomials (DDGS diets only) were used to evaluate treatment responses, and pen was the experimental unit. Increasing the SID Ile:Lys did not impact cumulative performance of pigs fed diets containing 20% DDGS (P &amp;gt; 0.175). Pigs fed CS had improved (P &amp;lt; 0.010) ADFI compared to pigs fed SID Ile:Lys of 65 and 75% and tended (P = 0.084) to have greater ADG than 55 and 75% Ile:Lys treatments. Feed efficiency did not differ due to treatment (P = 0.427). Increasing the SID Ile:Lys to 65% in 20% DDGS diets decreased back fat and increased loin depth (quadratic; P &amp;lt; 0.029) and tended to increase percent lean (quadratic; P = 0.076), but did not alter hot carcass weight (P &amp;gt; 0.428). Pigs fed CS diets had greater hot carcass weights (P &amp;lt; 0.031) compared to pigs fed DDGS diets except for pigs fed 60% Ile:Lys diets. These data suggest a SID Ile:Lys of 60% is required to maximize growth performance and SID Ile:Lys of 65% increases carcass traits in diets containing 20% DDGS.

Effect of feeding corn distillers dried grains with solubles naturally contaminated with deoxynivalenol on growth performance, meat quality, intestinal permeability, and utilization of energy and nutrients in broiler chickens

The objective of this experiment was to investigate the effect of feeding corn distillers dried grains with solubles (DDGS) naturally contaminated with deoxynivalenol (DON) on growth performance, meat quality, intestinal permeability, and utilization of energy and nutrients in broiler chickens. Two trials (growth and metabolism trials) were conducted. In the growth trial, a total of four hundred 7-day-old Ross 308 broiler chicks were allotted to 1 of 5 dietary treatments with 8 replicates in a completely randomized design. The diets were formulated to contain 5 inclusion levels of 0, 5, 10, 15, or 20% DON-contaminated DDGS in diets and were fed to birds for 21 d. Results indicated that increasing inclusion levels of DON-contaminated DDGS decreased (linear, P < 0.01) BW gain and feed efficiency of broiler chickens. The relative organ weights of the liver and breast were decreased (linear and quadratic, P < 0.05) by increasing inclusion levels of DON-contaminated DDGS in diets. The transepithelial electrical resistance values as a measure of intestinal permeability were decreased (linear, P < 0.05) by increasing inclusion levels of DON-contaminated DDGS in diets. In the metabolism trial, a total of twenty four 22-day-old Ross 308 broiler chickens were allotted to 1 of 3 dietary treatments consisting of 0, 10, or 20% inclusion of DON-contaminated DDGS in diets. Each treatment had 8 replicates. Increasing inclusion levels of DON-contaminated DDGS in diets decreased (linear and quadratic, P < 0.05) MEn (AMEn and TMEn) and apparent total tract retention of nitrogen and acid-hydrolyzed ether extract in diets. In conclusion, feeding diets containing more than 10% DON-contaminated DDGS to broiler chickens has negative effects on growth performance, intestinal permeability, and utilization of energy and nutrients in diets. Therefore, it is suggested that if DDGS is contaminated with DON, inclusion level of DDGS should be limited, possibly at less than 5.0% in broiler diets.