Dissipation Of Phenanthrene Research Articles

Combined Phenanthrene and Copper Pollution Imposed a Selective Pressure on the Rice Root-Associated Microbiome.

Combined organic and inorganic pollutants can greatly impact crops and microbes, but the interaction between coexisted pollutants and their effects on root-associated microbes under flooding conditions remains poorly understood. In this study, greenhouse experiments were conducted to investigate the individual and combined effects of phenanthrene (PHE) and copper (Cu) on rice uptake and root-associated microbial coping strategies. The results showed that more than 90% of phenanthrene was degraded, while the existence of Cu significantly reduced the dissipation of PHE in the rhizosphere, and the coexistence of phenanthrene and copper promoted their respective accumulation in plant roots. Copper played a dominant role in the interaction between these two chemicals. Microbes that can tolerate heavy metals and degrade PAHs, e.g., Herbaspirillum, Sphingobacteriales, and Saccharimonadales, were enriched in the contaminated soils. Additionally, microbes associated with redox processes reacted differently under polluted treatments. Fe reducers increased in Cu-treated soils, while sulfate reducers and methanogens were considerably inhibited under polluted treatments. In total, our results uncover the combined effect of heavy metals and polycyclic aromatic hydrocarbons on the assemblage of root-associated microbial communities in anaerobic environments and provide useful information for the selection of effective root-associated microbiomes to improve the resistance of common crops in contaminated sites.

Rhizosphere interactions between PAH-degrading bacteria and Pteris vittata L. on arsenic and phenanthrene dynamics and transformation

The pot experiment was conducted to monitor the dynamics in soil solution chemistry in order to determine the main rhizosphere processes determining As and PAH bioavailability when utilizing P. vittata and PAH-degrading bacteria to remediate co-contaminated soils. The result showed that P. vittata was capable of depleting soil solution As and increasing phenanthrene solubilization, and thus facilitating plant As uptake and phenanthrene dissipation. Bacterial inoculation enhanced soil phenanthrene dissipation and concurrently modified As bioavailability though increasing soil pH, facilitating Fe and Ca minerals solubilization, and accelerating organic matter decomposition. However, the main factors that determine As bioavailability in the rhizosphere considerably varied with plant genotypes. Upon bacterial inoculation, P and Fe strongly influenced As(V) availability and its uptake by the Guangxi accession, and DOC, Fe, and pH were the main parameters correlated with As(V) availability in the rhizosphere of the Hunan accession. Bacterial inoculation tended to stimulate As(V) reduction in the rhizosphere of P. vittata. Microbial-induced changes in Ca, S, and C cycling and pH were indicators of As(V) reduction. Although bacterial inoculation increased soil As and phenanthrene availability, striking differences in As and nutrients uptake and phenanthrene dissipation were observed between P. vittata genotypes. It is suggested that apart from the microbial transformation, plant genotypes and bacterial mediated plant nutrition are also the critical factors in controlling the fates of As and phenanthrene. Our results uncovered the interactions between P. vittata and PAH-degrading bacteria on rhizosphere properties and nutrients cycling regulating As and PAH availability and remediation efficiency.

Soil indigenous microorganisms weaken the synergy of Massilia sp. WF1 and Phanerochaete chrysosporium in phenanthrene biodegradation

Biodegradation is a promising way to reduce phenanthrene (PHE) in environment. PHE biodegradation by bioaugmentation of axenic and mixed cultures of Massilia sp. WF1 (a highly efficient PHE-degrading bacteria) and Phanerochaete chrysosporium (P. chrysosporium, an extensively researched model fungus in organic pollutant bioremediation) was investigated in aqueous and autoclaved/un-autoclaved soil cultures. In the liquid cultures, the strain WF1 could use PHE (ca. 10 mg L−1) as the sole carbon source, and the presence of d-fructose (500 mg L−1) had no obvious effect on its PHE degradation; while the opposite was observed for P. chrysosporium. The bioaugmentation of strain WF1 and P. chrysosporium co-culture showed the highest PHE-degradation efficiency, especially in the aqueous and the autoclaved soil (PHE, ca. 50 mg kg−1) cultures, indicating a synergistic interaction of the co-culture during PHE dissipation. It was further observed that the indigenous microorganisms (mainly the Gram-positive bacteria) played a dominant role during PHE biodegradation and showed an antagonistic action against the strain WF1-P. chrysosporium co-culture, which weakened the synergistic action of the co-culture in the un-autoclaved soil. Besides, the abundances of PAH-RHDα GP and nidA genes were negatively correlated with residual PHE in the soil. Our findings provide the scientific support for bioremediation of PAHs in environment.

Inoculation of Triticum Aestivum L. (Poaceae) with Plant-Growth-Promoting Fungi Alleviates Plant Oxidative Stress and Enhances Phenanthrene Dissipation in Soil

Polycyclic aromatic hydrocarbons (PAHs) are strong toxic compounds mainly released to the environment during combustion of fossil fuels, and have strong toxic effects on living organisms, with soil being one of their main reservoirs. High PAH levels in soils can interfere with plant growth and biomass production, causing several losses of diversity. In this study, we evaluated the effects of the co-inoculation of Trichoderma viride and Funneliformis mosseae on PAH dissipation and alleviation of oxidative stress in Triticum aestivum L. (wheat) plants growing in a phenanthrene-spiked soil. We determined the effect of single and dual fungal inoculation on phenanthrene dissipation rates, soil enzyme activities, dry biomass, antioxidant enzymes, lipid peroxidation, and organic acid exudation of plants growing in a soil spiked with phenanthrene at 500 and 1000 mg kg−1 soil. The co-inoculation with T. viride and F. mosseae resulted in a high phenanthrene dissipation from the soil. Also, dry biomass, soil enzymes, antioxidant response, organic acid exudation and phenanthrene content in roots were increased by the dual inoculation treatments, whereas lipid peroxidation and phenanthrene content in shoots were reduced. Our results show that the co-inoculation with these two soil fungi significantly promotes phenanthrene dissipation from soil and contributes to alleviating oxidative damage in wheat plants exposed to high levels of phenanthrene.

Assembly strategies of the wheat root-associated microbiome in soils contaminated with phenanthrene and copper

Plants can cope with stressful conditions by indirectly regulating root-associated microbial structures. However, the recruitment strategies of the root-associated microbiome in combined organic and inorganic contaminated soils are not well known, especially for common agricultural crops. In this study, we performed greenhouse experiments to investigate the interactive effects of joint copper (Cu) and phenanthrene (PHE) pollution on wheat growth and microbial detoxication processes. Results show that heavy metals did not affect PHE dissipation in the rhizosphere but significantly enhanced the accumulation of PHE in the endosphere. In contrast, the addition of PHE did not influence the absorption of Cu by wheat roots. Cu was the primary factor affecting the variation of microbial communities in cocontaminated treatments among each rhizocompartment while the interactive effects of combined pollutants were only detected in unplanted bulk soil. Microbes are known to degrade polycyclic aromatic hydrocarbons and tolerant heavy metal stress e.g. Novosphingobium, Sphingomonas, Sphingobium and Pseudomonas enriched in the contaminated treatments. Our results provide an integrated understanding of the synthetic effects of combined pollutants on the root-microbial assemblage process in plant-soil systems and offer useful information on the selection of effective bioremediating root-associated microbes for the application of self-remediation by common crops.

COMPARATIVE DISSIPATION OF ANTHRACENE AND PHENANTHRENE IN A PRISTINE TYPIC HAPLUDOLL SOIL

Polycyclic aromatic hydrocarbons (PAHs) are of great environmental concern due to their widespread occurrence and persistence. Anthracene and phenanthrene (C14H10) are two priority pollutants that are found in high concentrations in PAHs-contaminated surface soils. The objective of this study was to analyze the capability of endogenous soil microorganisms of a pristine soil of the Pampas region, Argentina, to dissipate two isomers: anthracene and phenanthrene. Nutrient availability and the effects of ryegrass (Lolium perenne L.) on these PAHs´ dissipation were also evaluated. After 100 days, both contaminants were significantly degraded in root-free soils by autochthonous microorganisms. L. perenne significantly enhanced microbial degradation. The dissipation of both pollutants in the rhizosphere was accompanied by higher values of total bacteria counts at the end of the experimental period. No biostimulation effect was observed. In all cases, the dissipation of phenanthrene was significantly higher than anthracene. These results point to the important role of indigenous PAH degrading microorganisms, even present in a non-polluted soil.

Effects of Plant Growth Promoting Rhizobacteria on Phytoremediation of Phenanthrene Contaminated Sodic Soil

The aim of the research is the dissipation of Phenanthrene in sodic soil in the presence of PGPR by two mucilagenous plant species (Trigonella foenum-graceum and Brassica juncea). To evaluate of affectivity of bioremediation in the presence of local sodic soil was artificially contaminated with the known different concentration of Phenanthrene separately to analyze the dissipation of Phenanthrene. Soil samples were taken after the 30 days of incubation in the presence of plant species and PGPR. Gas Chromatography result clearly indicate that Trigonella foenum-graceum (99%) is more efficient plant over the Brassica juncea (96%) is further indicate that PGPR having synergistic effect on phytoremediation of Phenanthrene.

Soil phenanthrene phytoremediation capacity in bacteria-assisted Spartina densiflora

Polycyclic aromatic hydrocarbons (PAH) have become a threat for the conservation of wetlands worldwide. The halophyte Spartina densiflora has shown to be potentially useful for soil phenanthrene phytoremediation, but no studies on bacteria-assisted hydrocarbon phytoremediation have been carried out with this halophyte. In this work, three phenanthrene-degrading endophytic bacteria were isolated from S. densiflora tissues and used for plant inoculation. Bacterial bioaugmentation treatments slightly improved S. densiflora growth, photosynthetic and fluorescence parameters. But endophyte-inoculated S. densiflora showed lower soil phenanthrene dissipation rates than non-inoculated S. densiflora (30% below) or even bulk soil (23% less). Our work demonstrates that endophytic inoculation on S. densiflora under greenhouse conditions with the selected PAH-degrading strains did not significantly increase inherent phenanthrene soil dissipation capacity of the halophyte. It would therefore be advisable to provide effective follow-up of bacterial colonization, survival and metabolic activity during phenanthrene soil phytoremediation.

Mycorrhizal alfalfa and surfactant affect the uptake and dissipation of phenanthrene in soil

A greenhouse experiment was conducted to compare the effects of biosurfactant Rhamnopyranoside and chemical surfactant Triton X-100 on the uptake of phenanthrene by mycorrhizal alfalfa (Medicago sativa L.) and phenanthrene dissipation in soil. Mycorrhizal treatment led to a higher phenanthrene accumulation in alfalfa and enhanced phenanthrene dissipation from the treated soil compared with non-mycorrhizal control. Inoculation combined with Rhamnopyranoside application gave the highest phenanthrene removal rate of 85.50% from treated soil and highest phenanthrene accumulation in plants (2.92 μg in root and 1.58 μg in shoot per pot) among all treatments. Besides, such combination treatment resulted in the highest freeze-dried biomass of plant (4.13 g for root and 3.31 g for shoot per pot), suggesting low toxicity for this treatment. These observations indicate co-effects of Rhamnopyranoside and inoculation may have potential as a biotechnological approach for decontamination of soil contaminated with phenanthrene.

Selenium enhances Conyza canadensis phytoremediation of polycyclic aromatic hydrocarbons in soil

To determine whether supplemental selenium (Se) enhances the tolerance of Conyza canadensis (C. canadensis) to polycyclic aromatic hydrocarbons (PAHs) and thereby accelerates PAH dissipation in soils. We examined the dissipation of different concentrations of two PAHs, phenanthrene (PHE) and pyrene (PYR), from contaminated soils after 60 days of growth of C. canadensis supplemented with or without a low level of Se (0.5 mg kg−1) in a pot culture experiment. PAH concentrations were 0, 50, 200, and 800 mg kg−1. Response variables included plant biomass, PAH accumulation in plants, soil microbial biomass carbon (MBC), root morphogenesis, antioxidant enzyme activity, and PHE/PYR dissipation. Plant biomass and root morphogenesis decreased with increasing PAH concentration, whereas malondialdehyde concentration, an indicator of oxidative stress, increased. Catalase activity, total superoxide dismutase activity, MBC, and the PAH dissipation ratio all increased with increasing PAH concentration until 800 mg kg−1. Se supplementation of soil increased the tolerance of C. canadensis to PAHs and significantly increased PAH phytoremediation after 60 days of plant growth. The PHE dissipation ratios with and without Se supplementation were 57 ± 3% and 28 ± 8%, respectively, and PYR dissipation ratios were 30 ± 7% and 24 ± 8%. The increase in the PAH dissipation ratio with Se supplementation was largest in PHE-contaminated soils at 800 mg kg−1. A low concentration of supplemental Se in soil enhances the dissipation of PHE and PYR by C. canadensis. This enhancement is due to an increased plant tolerance toward PAHs, which results in increased C. canadensis biomass, soil MBC, and decreased plant uptake of PAHs. This study suggests that Se supplementation can contribute to ongoing efforts to develop effective phytoremediation systems for soils contaminated with PAHs.

Effect of plant growth promoting bacterium; Pseudomonas putida UW4 inoculation on phytoremediation efficacy of monoculture and mixed culture of selected plant species for PAH and lead spiked soils

Plant growth promoting bacteria (PGPB) enhanced phytoremediation (PEP) is an attractive remedial strategy for the remediation of polycyclic aromatic hydrocarbon (PAH) and heavy metal (HM) contaminated sites. The effect of PGPB; Pseudomonas putida UW4 inoculation on the phytoremediation efficiency of Medicago sativa, Festuca arundinacea, Lolium perenne, and mixed plants (L. perenne and F. arundinacea) was assessed. This involved two contaminant treatments; “PAH” (phenanthrene; 300 mg·kg−1, fluoranthene; 200 mg·kg−1, and benzo[a]pyrene; 5 mg·kg−1) and “PAH + HM” (‘PAH’ treatments +100 mg of Pb/kg). PGPB inoculation significantly enhanced root biomass yield of F. arundinacea in PAH treatment, and the mixed plant shoot biomass and L. perenne root biomass yields of the PAH + HM treatment. PGPB significantly enhanced dissipation of phenanthrene and fluoranthene for M. sativa-PAH + PGPB treatment and fluoranthene for F. arundinacea-PAH + HM + PGPB treatment. In others, PGPB inoculation either had no impact or inhibited PAH dissipation. PAH dissipation for the single and mixed plant treatments with PGPB inoculation were not different. The efficiency of PEP is dependent on different factors such as PGPB inoculum biomass, plant species, plant–microbe specificity and type of contaminants. Exploiting PEP technology would require proper understanding of plant tolerance and growth promoting mechanisms, and rhizosphere activities.

The responding and ecological contribution of biofilm-leaves of submerged macrophytes on phenanthrene dissipation in sediments

The bacterial communities and ecological contribution of biofilm-leaves of the Vallisneria natans (VN), Hydrilla verticillata (HV) and artificial plant (AP) settled in sediments with different polluted levels of phenanthrene were investigated by high-throughput sequencing in different growth periods. There was no significant difference among the detected Alpha diversity indices based on three classification, attached surface, spiking concentration and incubation time. While Beta diversity analysis assessed by PCoA on operational taxonomic units (OTU) indicated that bacterial community structures were significantly influenced in order of attached surface > incubation time > spiking concentration of phenanthrene in sediment. Moreover, the results of hierarchical dendrograms and heat maps at genus level were consistent with PCoA analysis. We speculated that the weak influence of phenanthrene spiking concentration in sediment might be related to lower concentration and smaller concentration gradient of phenanthrene in leaves. Meanwhile, difference analysis suggested that attached surface was inclined to influence the rare genera up to significant level than incubation time. In general, the results proved that phenanthrene concentrations, submerged macrophytes categories and incubation time did influence the bacterial community of biofilm-leaves. In turn, results also showed a non-negligible ecological contribution of biofilm-leaves in dissipating the phenanthrene in sediments (>13.2%–17.1%) in contrast with rhizosphere remediation (2.5%–3.2% for HV and 9.9%–10.6% for VN).

Enhanced arsenic uptake and polycyclic aromatic hydrocarbon (PAH)-dissipation using Pteris vittata L. and a PAH-degrading bacterium

This study examined the effects of P. vittata and a polycyclic aromatic hydrocarbon (PAH)-degrading bacterium (Alcaligenes sp.) on arsenic (As) uptake and phenanthrene dissipation. Bacterial inoculation substantially increased As accumulation in plants by 27.8% (frond) and 27.5% (root) at 60d, respectively, compared with the non-inoculated treatment, although temporal change of As translocation and reduction in plants was observed. Bacterial inoculation positively affected plants by improving growth, nutrition and antioxidative activities, and helped to modify soil As availability to the plants, which may benefit in plant tolerance and As accumulation. Plant and bacteria association enhanced phenanthrene dissipation from the soil, with the highest dissipation rate of 96.4% at 60d in the rhizosphere, which might be associated with enhanced bacterial population and activity inspired by the growth of plant. The result reveals that combination of P. vittata and PAH-degrading bacteria can promote As accumulation and phenanthrene dissipation, and can be exploited as a promising strategy for As and PAH co-contamination remediation.

Effect of wetland plants and bacterial inoculation on dissipation of phenanthrene

ABSTRACTThis study attempts to evaluate the capacity of wetland plants' ability to dissipate phenanthrene (PHE) under waterlogged conditions. The results indicate that Typha latifolia and Vetiveria zizanioides may efficiently degrade PHE, and were much more effective when under combined plant cultivation with the inoculation of Pseudomonas frederiksbergensis (ATCC BAA-257) . Concentrations of PHE declined from 200 to less than 52 mg kg−1 in all treatments with plant cultivation. At the end of the experimental period, PHE was undetectable in combined plant cultivation in the presence of bacteria inoculation. Microbial biomass C(carbon), N(nitrogen), and P(phosphate) were significantly different (p < 0.05) in the presence and absence of bacteria inoculation with bacteria inoculation significantly (p < 0.05) increased microbial biomass P. The presence of bacteria inoculation and different plant species significantly (p < 0.05) decreased the PHE concentrations in the microcosms. The inoculation of bacteria and release of exudates from plant roots further enhanced the dissipation of PHE in sand. Concentrations of citric and malic acids were decreased up to 69% in bacteria-inoculated treatments, showing large citric and malic acids serving as a food source and growth substrate for bacteria.

Interactions between Pteris vittata L. genotypes and a polycyclic aromatic hydrocarbon (PAH)-degrading bacterium (Alcaligenes sp.) in arsenic uptake and PAH-dissipation

The effects of two Pteris vittata L. accessions and a polycyclic aromatic hydrocarbon (PAH)-degrading bacterium (Alcaligenes sp.) on arsenic (As) uptake and phenanthrene dissipation were studied. The Alcaligenes sp. survived in the rhizosphere and improved soil As bioavailability with co-exposure. However, bacterial inoculation altered Pteris vittata L. stress tolerance, and substantially affected the As distribution in the rhizosphere of the two P. vittata accessions. Bacterial inoculation was beneficial to protect the Guangxi accession against the toxic effects, and significantly increased plant As and phenanthrene removal ratios by 27.8% and 2.89%, respectively. In contrast, As removal was reduced by 29.8% in the Hunan accession, when compared with corresponding non-inoculated treatments. We conclude that plant genotype selection is critically important for successful microorganism-assisted phytoremediation of soil co-contaminated with As and PAHs, and appropriate genotype selection may enhance remediation efficiency.

Low effect of phenanthrene bioaccessibility on its biodegradation in diffusely contaminated soil

This study focused on the role of bioaccessibility in the phenanthrene (PHE) biodegradation in diffusely contaminated soil, by combining chemical and microbiological approaches. First, we determined PHE dissipation rates and PHE sorption/desorption isotherms for two soils (PPY and Pv) presenting similar chronic PAH contamination, but different physico-chemical properties. Our results revealed that the PHE dissipation rate was significantly higher in the Pv soil compared to the PPY soil, while PHE sorption/desorption isotherms were similar. Interestingly, increases of PHE desorption and potentially of PHE bioaccessibility were observed for both soils when adding rhamnolipids (biosurfactants produced by Pseudomonas aeruginosa). Second, using 13C-PHE incubated in the same soils, we analyzed the PHE degrading bacterial communities. The combination of stable isotope probing (DNA-SIP) and 16S rRNA gene pyrosequencing revealed that Betaproteobacteria were the main PHE degraders in the Pv soil, while a higher bacterial diversity (Alpha-, Beta-, Gammaproteobacteria and Actinobacteria) was involved in PHE degradation in the PPY soil. The amendment of biosurfactants commonly used in biostimulation methods (i.e. rhamnolipids) to the two soils clearly modified the PHE sorption/desorption isotherms, but had no significant impact on PHE degradation rates and PHE-degraders identity. These results demonstrated that increasing the bioaccessibility of PHE has a low impact on its degradation and on the functional populations involved in this degradation.

Dissipation of Phenanthrene and Anthracene from Soil with Increasing Salt Content Amended with Wastewater Sludge

Dissipation of Phenanthrene and Anthracene from Soil with Increasing Salt Content Amended with Wastewater Sludge

Alteration in successional trajectories of bacterioplankton communities in response to co-exposure of cadmium and phenanthrene in coastal water microcosms

Coexistence of heavy metals and organic contaminants in coastal ecosystems may lead to complicated circumstances in ecotoxicological assessment for biological communities due to potential interactions of contaminants. Consequences of metals and polycyclic aromatic hydrocarbons (PAHs) co-contamination on coastal marine microbes at the community level were paid less attention. We chose cadmium (Cd) and phenanthrene (PHE) as representatives of metals and PAHs, respectively, and mimicked contaminations using coastal water microcosms spiked with Cd (1 mg/L), PHE (1 mg/L), and their mixture over two weeks. 16S rRNA gene amplicon sequencing was used to compare individual and cumulative effects of Cd and PHE on temporal succession of bacterioplankton communities. Although we found dramatic impacts of dimethylsulfoxide (DMSO, used as a carrier solvent for PHE) on bacterial α-diversity and composition, the individual and cumulative effects of Cd and PHE on bacterial α-diversity were temporally variable showing an antagonistic pattern at early stage in the presence of DMSO. Temporal succession of bacterial community composition (BCC) was associated with temporal variability of water physicochemical parameters, each of which explained more variation in BCC than two target contaminants did. However, Cd, PHE, and their mixture distinctly altered the successional trajectories of BCC, while only the effect of Cd was retained at the end of experiment, suggesting certain resilience in BCC after the complete dissipation of PHE along the temporal trajectory. Moreover, bacterial assemblages at the genus level associated with the target contaminants were highly time-dependent and more unpredictable in the co-contamination group, in which some genera possessing hydrocarbon-degrading members might contribute to PHE degradation. These results provide preliminary insights into how co-exposure of Cd and PHE phylogenetically alters successional trajectories of bacterioplankton communities in the manipulated coastal water microcosms.

Dynamics of PAHs and derived organic compounds in a soil-plant mesocosm spiked with 13C-phenanthrene

Polycyclic Aromatic Hydrocarbons (PAHs) are ubiquitous and persistent soil pollutants. Their fate and the influence of the plant rhizosphere on their dynamics has been extensively studied, but studies mainly focused on their dissipation rate. We conducted a plant-soil mesocosm experiment to study the fate and distribution of PAHs or derived compounds in the extractable fraction, the residual soil, the shoot biomass and the root biomass. The experiment was conducted for 21 days using ryegrass and a forest soil spiked with 13C-labeled phenanthrene (PHE), using combined IRMS and NanoSIMS for analyses. Almost 90% of the initial extractable PHE content was dissipated within 3 weeks, but no rhizospheric effect was highlighted on PHE dissipation. More than 40% of 13C-PHE was still in the soil at the end of the experiment, but not as PHE or PAH-derived compounds. Therefore it was under the form of new compounds (metabolites) and/or had been incorporated into the microbial biomass. About 0.36% of the initial 13C-PHE was recovered in the root and shoot tissues, representing similar 13C enrichment (E13C) as in the soil (E13C ≈ 0.04 at.%). Using NanoSIMS, 13C was also localized at the microscale in the roots and their close environment. Global 13C enrichment confirmed the results obtained by IRMS. Some hotspots of 13C enrichment were found, with a high 32S/12C14N ratio. Comparing the ratios, sizes and shapes of these hotspots suggested that they could be bacteria.

Rhizodegradation potential and tolerance of Avicennia marina (Forsk.) Vierh in phenanthrene and pyrene contaminated sediments

A pot experiment was conducted to investigate the dissipation of phenanthrene and pyrene in spiked sediments with presence of Avicennia marina (Forsk.) Vierh. The rhizosphere environment was set up using a self-design nylon rhizo-bag which divided the sediment into the rhizosphere and non-rhizosphere. Results showed that the dissipation of phenanthrene and pyrene were significantly enhanced in the rhizosphere compared with non-rhizosphere sediments. Plant roots promoted dissipation significantly greater than the contribution of direct plant uptake and accumulation of phenanthrene and pyrene. The activities of antioxidant and detoxification enzymes in roots and leaves significantly increased against oxidative stress with increasing PAH concentrations. Furthermore, a significant relationship (R2>0.91) between dissolved organic carbon (DOC) concentrations and the residual of PAHs in rhizosphere and non-rhizosphere sediments was observed after 120days planting. Results indicated that rhizome mediation with A. marina is a useful approach to promote the depletion of PAHs in contaminated mangrove sediments.