Disposal Practices Research Articles

Evaluation of a Menstrual Hygiene Education Program for Adolescent Girls in Rural India.

To assess the impact of a pharmacist-mediated education program on menstrual hygiene among adolescent girls in and around Balagangadaranatha (BG) Nagara, India. Pre- and postintervention study conducted over 6months. Adolescent girls in India, especially in rural areas, may lack proper knowledge and a supportive atmosphere to deal with menstruation-related concerns. Many myths and taboos also make it difficult or impossible to find accurate information about menstruation and menstrual hygiene. The sample included 585 female high school students who had begun menstruation and lived in and around BG Nagara, India. An educational training session provided information about the menstrual cycle, sanitary products, proper disposal practices, and menstrual disorders. Information addressed the psychological and social dimensions of menstruation to mitigate stigma. Educational leaflets about personal menstruation hygiene practices were also provided. Pre- and posttests were administered to assess participants' knowledge of and practices regarding menstrual hygiene. Pretest scores indicated that 43.8%(n= 164) of participants had good knowledge of menstruation, 86%(n= 503) used sanitary pads, and 73.2%(n= 428) thought that increasing nutritious food was necessary during menstruation. Posttest scores indicated an increase in all areas (p< .001). Health education was associated with an improvement in female high school students' knowledge of and practices related to menstrual hygiene. More menstrual health education strategies and awareness programs are needed to improve the knowledge and health of adolescent girls.

Molecular Profiling of Rice Straw Degradability Discrepancy in Stropharia rugosoannulata Core Germplasm.

The rice-S. rugosoannulata pattern is a rapidly growing agricultural practice for straw disposal and mushroom production in China. However, different S. rugosoannulata strains show a large variation in rice straw degradability. Here, we constructed a core collection of S. rugosoannulata containing 14 strains with rich genetic diversity. The molecular profiling of the lignocellulose degradability discrepancy of S. rugosoannulata strains was then explored using enzyme activity assays and transcriptome analysis. The results indicated that mycelial growth rate, lignocellulolytic enzyme activities, and rice straw degradability differed widely among the S. rugosoannulata core strains. The genes encoding lignin modifying and degrading auxiliary enzymes, oxidases, glycoside hydrolases, and detoxification proteins were differentially expressed between two representative S. rugosoannulata strains, resulting in differences in their lignocellulolytic enzyme activities and further causing differences in lignocellulose degradability. This study is useful to improve the production efficiency of S. rugosoannulata and promote the recycling of rice straw.

BATERIAS DE CARROS ELÉTRICOS: MANUTENÇÕES E DESCARTES

The rapid growth in the adoption of electric vehicles brings to light challenges related to the maintenance and disposal of batteries, which are essential components for the operation of these vehicles. The central issue addressed is the sustainability of the battery life cycle, considering both the efficiency of their maintenance and the environmental impacts resulting from inadequate disposal. The context involves the transition to a greener economy and the demands for clean technologies, which makes it essential to discuss appropriate practices to extend the useful life of batteries and ensure their safe disposal. The main objective of this study is to analyze the maintenance practices of electric car batteries and disposal strategies, focusing on minimizing environmental impacts and optimizing the life cycle of components. The research seeks to identify effective methods to extend the useful life of batteries, in addition to evaluating existing recycling policies and processes. The methodology used includes a literature review of current battery maintenance and disposal practices, in addition to the analysis of case studies that exemplify different approaches and results in international contexts. The research also incorporates interviews with industry experts, aiming to obtain a comprehensive and updated view of best practices and challenges faced. This work contributes to the discussion on sustainability in the automotive sector and to the formulation of more efficient policies for the management of technological waste.

Practice Towards Storage of Unused and Disposal of Expired Medicines among Households in Dharan, Nepal

Introduction: Improper disposal of unused medications has been a public health problem, as unused drug adversely effects on health and environment. Objective: The main objective of this study was to assess the extent of unused medications disposal knowledge, attitude, and practice of among household in Dharan, Province 1, Nepal. Methods: A survey based descriptive study was used. Households were conveniently selected and interviewed using structured questionnaires. Attitudes and practices regarding medicine disposal and storage were asked. The data were analyzed by SPSS version 20.0 software and presented descriptively. Results: Among 380 household surveyed, majority of respondents (82%) reported storing unused medications in their home and only 5% reported returning medication to a pharmacy. Predominant reason for medication non-used was disease or symptoms felt improved (80 %). Less than half of respondents (45%) had check the expiry date of their medicine before purchasing. Most respondents (88%) thought that improper disposal of medicine may cause harmful effects to health and environment. Conclusion: Incorrect practices were found in Dharan families with regard to the storage and disposal of unused medications. Along with a comprehensive media campaign to inform consumers about safe medication disposal techniques, an efficient method for disposing of leftover medications should be offered. This system should be supported by local pharmacist.

Environmental persistence, bioaccumulation, and hazards of chemicals in e-cigarette e-liquids: short-listing chemicals for risk assessments

Background/MethodsIncreased use and sales of e-cigarettes raises concerns about the potential environmental impacts throughout their life-cycle. However, few available research studies focus on the environmental impacts and ecotoxicity of e-cigarettes....

The insensitivity of ‘sensitive care’: the bureaucracy of pregnancy tissue disposal in England, UK

The practices surrounding pregnancy ends and pregnancy remains shift and change depending on the cultural and historical context. Based on ethnographic research in one group NHS Hospital organisation in England, the paper explores what practices around pregnancy remains reveal about the values afforded the material in different contexts by different actors and the moments when these intersect. It argues that framing miscarriage as bereavement helps to structure caregiving in clinical settings and that clinical practices produce foetal personhood in ways that may not be in keeping with women’s notions of their pregnancy material. It illustrates that hospital practices contain notions of value which become legitimated as the appropriate approach with consequences for normativity. This may lead to women feeling isolated and abnormal when their approach is at odds with that of the clinic. Through an exploration of how women encounter and negotiate disposal practices, the paper argues that current practice requires revision to flexibly respond to diversity but also shifting meaning and values attributed to these experiences and materials.

Insights into Drug Donation Practices and Public Perceptions in Saudi Arabia

Background: Effective medication management, disposal, and donation are vital for public health and environmental sustainability. Improper handling of medications can lead to drug misuse, accidental poisoning, and environmental damage. This study examines current practices and challenges in Saudi Arabia, identifying opportunities for improvement. Method: A cross-sectional survey conducted in October and November 2023 targeted the general population in Saudi Arabia. This study employed convenience sampling to explore medication usage, storage, disposal practices, and awareness of donation procedures. Data were analyzed through both descriptive and inferential statistical methods. Results: This study involved 430 respondents. Of these, 73.0% held a university degree, yet 66.3% were unaware of drug donation programs, and 84.2% lacked knowledge about proper medication disposal. Despite this, 71.4% believed that drug donation programs positively impact healthcare, and 87.9% saw them as reducing drug waste and environmental pollution. However, 48.1% expressed concerns about the potential misuse of donated medicines. Awareness and knowledge were significantly higher among participants aged 30 and above. Conclusions: The findings highlight the need for enhanced public awareness, clear medication disposal guidelines, and ethically governed donation practices in Saudi Arabia. These measures can improve healthcare outcomes, protect the environment, and support global health and sustainability goals.

Perceived social, economic, environmental and health effects of solid waste management practices in logia town, afar, ethiopia

Improper solid waste management in urban and semi-urban areas has led to adverse impacts on the environment, public health, and the economic well-being of communities. Therefore, this study analyzes the status of household solid waste management practices and their effects in Logia Town, Afar, Ethiopia. To achieve this objective, the study utilizes both descriptive and econometric models (binary logistic regression model). The descriptive results of the study revealed that there is general awareness of the importance of solid waste management (SWM) and its impact on the environment, economy, and human health among the respondents. Regarding respondents’ attitudes, the majority of households understand the responsibility of proper waste disposal and its impact. However, in terms of solid waste management practices, 80% of households do not properly segregate their waste, 59.3% do not separate solid waste (SW) before disposal, and 74% of households engage in improper disposal practices. About 95.9% of respondents were aware that improper waste management can lead to diseases such as diarrhea, typhoid, and cholera. Around 65% of the sampled households considered solid waste as a source of wealth, and, as a result, 56% of them reuse, recycle, and reduce waste. This indicates that solid waste has economic value for society. Regarding the environmental impact of SW, about 89% of respondents argued that SW adversely affects the environment by polluting rivers, lakes, wells, and air. This environmental impact negatively affects the well-being and economic status of the community. The binary logistic regression model found that having adequate solid waste landfills, access to door-to-door solid waste collection, knowledge of SWM rules and regulations, treating waste as a resource, and understanding the 3Rs (recycle, reuse, reduce) had positive and significant effects on the adoption of proper solid waste management practices in the study area. Therefore, to reduce undesirable effects and improve the value of SW, the relevant authorities should provide adequate solid waste landfills and door-to-door collection services, create awareness about SWM rules and regulations, and promote knowledge of the 3Rs.

Infrastructures of Surveillance and Control in the Invisible City of Waste

ABSTRACT This paper offers a situated analysis of infrastructures of surveillance and control in Rotterdam through Latour & Hermant’s concept of the oligopticon. Oligopticons are networked devices and their supporting infrastructures that render the city in extremely narrow but very clear representations, in this case as a city of waste hotspots. In Rotterdam, public management is designing data-hungry oligopticons that map, control and intervene in the illegal disposal of waste and litter. An analysis of two vignettes from the author’s ethnographic fieldwork finds that these oligopticons do more than simply make visible and control these practices of waste disposal: they instead engage in punitive politics that frames those who illegally dispose of waste as exceptions to a normalizing way of life, fighting not waste but difference. Through ethnographic comparison, this paper aims to evoke thought, imagination and reflection in readers regarding the consequences of infrastructures of surveillance and control and the intimate knowledge that may escape these infrastructures.

Disposal practices, knowledge and attitude of adult patients visiting outpatient pharmacy services towards unused medicines in Debre Tabor, Northwest Ethiopia: a descriptive cross-sectional study

ObjectiveAccumulation at home and improper disposal of unused medicines pose a major public health problem. Knowledge and attitude of patients may contribute to accumulation and disposal practice of unused medicines....

E-Waste Management Practices Through the Eyes of Responsible Departments at Malaysian Public Universities

Electronic and electrical waste (E-waste) has been identified as the fastest-growing waste stream in the world. In Malaysia, forecasts indicate that it will soon reach 24.5 million units of e-waste by the year 2025. Today, higher learning institutions also contribute to the rapidly growing threat of e-waste. Besides mobile phones, the usage of Information and Communication Technology (ICT) equipment at universities keeps increasing and contributes to the increased quantity of e-waste, while the handling and disposal methods of e-waste still need to be improved. The purpose of this paper is to examine the current e-waste management system as well as to investigate the critical challenges constraining e-waste management at public universities in Malaysia. By using a qualitative approach, a number of interview sessions have been conducted with the responsible departments at six established public universities in Malaysia. It has been found that currently only e-waste generated from used electric and electronic equipment (EEE) which have been declared as university assets are being managed by the responsible departments at the six universities. All six universities are using the standard process of handling their e-waste since every single process needs to comply with the requirements of the Ministry of Finance (MOF). This remains the main constraint to flexibility or improvement of the current system. The universities are also facing some challenges, among others, lack of awareness and knowledge on how to properly handle e-waste among university citizens, no clear sustainable policy at the university, lack of facilities within the university compound to support the proper practice of e-waste disposal as well as lack of education and related training provided to the campus citizens. This paper also provides some recommendations for the improvement of e-waste management towards sustainability and offers an agenda for future research.

A convenient strategy for mitigating microplastics in wastewater treatment using natural light and ZnO nanoparticles as photocatalysts: A mechanistic study

Polypropylene microplastics (PPMPs) are one of the major emerging contaminants in the ecosystem due to their frequent usage and improper disposal practices. These PPMPs enter ecosystems via wastewater effluent plants and cause severe environmental health issues. In addition, quantifying PPMPs smaller than 50 μm in wastewater plant extraction is very difficult. Thus, the current study was designed to mitigate the PPMPs using zinc oxide nanoparticles (ZnONPs) as a photocatalyst under sunlight. The photocatalytic reaction was examined using spectroscopic techniques and microscopic imaging. The findings indicated that the weight loss percentage of PPMPs increased, and a decrease in UV–Vis DRS peak intensities was observed. The spectroscopic results elucidated the formation of free radicals, which affect the PPMPs and lead to the formation of carbonyl, allylic, and unsaturated groups. Further, EDS reports clarified that there is increased oxygen content due to the photooxidation process and the disintegration of the polymer chain owing to decreased carbon levels. Overall, ZnO photocatalyst absorbs photons from the visible spectrum of sunlight and forms free radicals, which affect the PPMPs to initiate polymer deterioration. Also, the current study revealed the mechanistic pathway of PPMP degradation under the photocatalytic reaction as proposed in the results obtained above.

Extent and causes of unused medications among patients in rural Ethiopia: a prospective multicenter cohort study

BackgroundMedication waste poses health, economic, and environmental challenges. However, the extent among patients living in rural areas is underexplored. This study assessed the proportion of prescribed medications remaining unused by patients living in rural areas of Ethiopia, and identify the causes thereof and disposal practices.MethodsA prospective multicenter cohort study was conducted in 5 rural health centers in Ethiopia. Patients (≥ 18 years), who received a prescription for acute or chronic medication for pick up from the outpatient pharmacy were included. After 3 months, participants received a house visit by a health employee during which a questionnaire was verbally administered to assess the quantity of unused medication, reason thereof, and disposal practices used. Data were analyzed using descriptive statistics and multivariate logistic regression to identify factors associated with presence of unused medications.ResultsIn total, 178 patients participated. Up to 136 out of 601 (22.6%) dispensed medications ended up unused, mainly antibiotics and analgesics, with an average economic value of $0.37. Of 178 patients, 72 (40.4%) ended up with unused medication, and 15 (8.4%) did not use 80% or more of the prescribed quantity. Early discontinuation of therapy was the main reason (61.8%) for patients’ ending with unused medication. Patients reported to primarily dispose of unused medication either through the toilet (43.6%), household garbage (22.7%), burning (13.6%), or returning it to the pharmacy (2.7%). Medications dispensed to be administered with two or more-unit doses at a time were more likely to remain unused (adjusted OR 1.6 [1.0–3.4]) compared to medications dispensed to be administered one-unit dose.ConclusionA substantial amount of prescribed medications remains unused by patients in rural areas, frequently not properly disposed. Interventions are needed to ensure medications are not wasted and reduce the unwanted consequences.

Environmental and Health Impacts Resulting From Burning Solid Waste Near Residential Areas in Diyala Governorate, Iraq

General Background: Rapid development, population growth, and improper disposal of municipal solid waste are prevalent issues in developing countries, leading to significant environmental and health challenges. Specific Background: In Iraq’s Diyala Governorate, the absence of integrated waste management exacerbates pollution through waste burning and heavy metal contamination, posing risks to nearby residential areas. Knowledge Gap: While previous studies have addressed waste management in developing countries, there is limited research on how local waste disposal practices impact air, soil, and water quality in specific areas of Diyala. Aims: This study evaluates the air quality and levels of heavy metals in soil and water near five landfill sites in Diyala Governorate: Kanaan, Al-Khalis, Baladruz, Khanaqin, and Hamrin, aiming to assess the pollution levels and the potential health risks to nearby communities. Results: The findings reveal significant differences across the sites, with the highest pollutant concentrations in Kanaan for air (40±445.8), soil (22.18±35.64), and water (3.037±3.146), while Hamrin recorded the lowest concentrations. All sites demonstrated statistically significant differences (P &lt; 0.05). Novelty: This study provides the first comprehensive analysis of the environmental impact of waste disposal practices in Diyala, linking pollutant concentrations with specific landfill sites. Implications: The results underscore the need for improved waste management strategies, including monitoring, waste sorting, and recycling initiatives, to mitigate the negative effects on public health and the environment, particularly in areas near waste dumps. Highlights: Pollution levels vary significantly across Diyala's landfill sites. Kanaan site has the highest air, soil, and water contamination. Better waste management is needed to reduce environmental and health risks. Keywords: Municipal waste, environmental pollution, heavy metals, Diyala Governorate, landfill impact

Life Cycle of LiFePO4 Batteries: Production, Recycling, and Market Trends.

Significant attention has focused on olivine-structured LiFePO4 (LFP) as a promising cathode active material (CAM) for lithium-ion batteries. This iron-based compound offers advantages over commonly used Co and Ni due to its lower toxicity abundance, and cost-effectiveness. Despite its current commercial use in energy storage technology, there remains a need for cost-effective production methods to create electrochemically active LiFePO4. Consequently, there is ongoing interest in developing innovative approaches for LiFePO4 production. While LFP batteries exhibit significant thermal stability, cycling performance, and environmental benefits, their growing adoption has increased battery disposal rates. Improper disposal practices for waste LFP batteries result in environmental degradation and the depletion of valuable resources. This review comprehensively examines diverse synthesis approaches for generating LFP powders, encompassing conventional methodologies alongside novel procedures. Furthermore, it conducts an in-depth assessment of the methodologies employed in recycling waste LFP batteries. Moreover, it emphasizes the importance of LFP cathode recycling and investigates pretreatment techniques to enhance understanding. Additionally, it provides valuable insights into the recycling process of used LFP batteries, aiming to raise awareness regarding the market for retired LFP batteries and advocate for the enduring sustainability of lithium-ion batteries.

Knowledge and practices on home medication storage and disposal in Syria: a population-based, cross-sectional study

BackgroundMedications are commonly found in every household. In Syria, where healthcare infrastructure faces significant challenges, improper storage can lead to reduced medication efficacy and increased health risks. Additionally, inappropriate disposal practices pose serious environmental concerns, especially in a region already grappling with environmental degradation. This study aimed to evaluate the knowledge, and practices of Syrian individuals regarding the appropriate storage and disposal of home medications.MethodA cross-sectional study was conducted from September 22 to October 20, 2023, using an online survey distributed via social media apps (WhatsApp, Facebook, and Telegram) in Syria. The questionnaire, adapted from a similar study in Saudi Arabia, was translated to Arabic using the forward–backward translation technique. A convenience sampling technique was used. Inclusion criteria were participants over eighteen with expired or unused medications at home. Descriptive statistics, Chi-square tests, and binary logistic regression analysis were performed using SPSS version 27, with statistical significance defined as p < 0.05.ResultsA total of 2,217 responses were analyzed, with the majority of participants being female (79%) and aged between 21–30 years (56%). The study found that 90.7% of respondents dispose of expired medications in the garbage, while 95% keep medications in their original containers. Additionally, 30% consult pharmacists for storage instructions, and 64% read storage information on medication leaflets. Logistic regression analysis revealed that males were less likely to check expiration dates before use (OR: 0.58, 95% CI: 0.40–0.84) and periodically (OR: 0.68, 95% CI: 0.54–0.85). Participants aged 31–40 years were less likely to check expiration dates periodically (OR: 0.46, 95% CI: 0.31–0.68), while those with children under six years old were more likely to do so (OR: 1.29, 95% CI: 1.01–1.67). Males were also less likely to read storage instructions (OR: 0.61, 95% CI: 0.50–0.76) but more likely to ask pharmacists about them (OR: 1.26, 95% CI: 1.01–1.57). There was no significant difference in these practices between medical professionals and the general population.ConclusionThis study reveals gaps in the practices related to the storage and disposal of medications among the Syrian public. The findings suggest a need for targeted public education and awareness programs to improve these practices and reduce the associated health and environmental risks.

Evaluating Household Hazardous Waste Generation, Composition, and Health Risks in an Urban Municipality

This study aimed to assess the generation rate and composition of household hazardous waste (HHW) in Nakhon Si Thammarat Municipality, Thailand. A questionnaire survey was conducted to collect data on HHW generation and disposal practices from households in the municipality. Soil samples were collected and analyzed for heavy metals (cadmium, chromium, mercury, nickel, and lead) and PCBs contamination. The potential health risks from ingestion exposure associated with heavy metals and polychlorinated biphenyls (PCBs) contamination in the soil were evaluated. The result revealed that in 2023, the HHW generation rate was 11.95 kg/household/year, with the highest percentages occurring of electronic waste (17.40%), fluorescent lamps (16.98%), spray cans (12.38%), cleaning products (11.30%), and engine oil and lubricant oil products (9.87%). The majority of residents (92.90%) disposed of hazardous waste and general waste together in public waste containers. However, the health risk assessment indicated that the levels of heavy metals and PCBs in the soil were within the safe acceptable range for residents, with a total lifetime cancer risk from ingestion exposure of less than 1E-06 for both adults and children. The findings highlight the need for improved waste separation practices and the implementation of effective hazardous waste management strategies to minimize potential health risks and environmental impacts. This study serves as a basis for developing targeted interventions and policies to enhance household hazardous waste management in the municipalities and similar urban areas in Thailand. Doi: 10.28991/HEF-2024-05-03-011 Full Text: PDF

Composition and Characteristics of Faecal Sludge from Various Onsite Pit Technologies and User Categories in Dar es Salaam, Tanzania

This study investigates the composition and characteristics of faecal sludge (FS) from different pit technologies (cesspits, pit latrines, septic tanks) and user categories (commercial, industrial, institutional, and residential) in Dar es Salaam, Tanzania, with a view for informed treatment and resource recovery strategies. FS samples exhibited varying concentrations of pH, electrical conductivity (EC), total solids (TS), and volatile solids (VS), COD and nutrients. pH values ranged from 2.83 to 8.41, with no statistically significant differences based on pit technology type or user category. However, the extremely low pH observed in samples from industrial pits suggests possible disposal of industrial chemicals in these sanitation systems. EC levels were notably higher in pit latrines and residential areas, likely due to waste disposal practices in homes, with concentrations 3-5 times above regulatory limits, raising concerns about their impact on treatment effectiveness and re-use potential. TS and VS concentrations also varied, with cesspits showing the highest TS levels and pit latrines exhibiting higher VS, indicating fresher sludge and potential for biogas production, as reflected by a VS/TS ratio ranging from 53.32% to 70.42%. Nutrient analysis revealed elevated levels of phosphate, nitrate and ammonia, particularly in pit latrines and residential pits, highlighting both resource recovery opportunities and challenges for treatment processes. While FS generally fell into the dilute category (TS &lt; 3%), the high concentrations of other parameters underscore the need for a larger treatment footprint per unit volume of FS. The VS/TS ratio suggests that FS is not fully stabilized, indicating potential for further degradation and resource recovery. Additionally, the characteristics of FS suggest potential opportunities for deriving fertilizer and solid fuel. However, ensuring the hygiene of the sludge is crucial for its safe use, particularly in agricultural applications, and further studies are needed to confirm its sanitation.

Assessment of Microplastic Pollution in Fresh Fish and Pindang Fish and its Potential Health Hazards in Coastal Communities of Banyuwangi Regency, Indonesia

This study aimed to analyze the microplastic contamination of fresh and pindang fish and its health impact on the coast of Muncar, Banyuwangi Regency, Indonesia. In this study, a total of 115 respondents participated, providing questionnaire data on their fish consumption habits and health problems. Subsequently, spearman’s correlation coefficient, a non-parametric statistical test, was used to analyze the questionnaire data. This study also included 100 samples of marine fish, consisting of 89 fresh fish and 11 pindang fish from various types of marine species. The content of microplastic polymers detected through FTIR (Fourier-Transform Infrared Spectroscopy) was around 3-5 microplastic polymers/fish samples, and the most dominant were Polyethylene, Polyester, Polycaprolactam (Nylon 6) and Polyamide. This study showed that 94 percent of fish samples contained microplastics and only 6 percent of samples did not contain microplastics. The intensity of pindang fish consumption was positively correlated with respondents’ health symptoms and problems. Subsequently, implementing effective waste management systems and educational programs in the coastal areas is crucial in reducing the pollution of seawater resulting from inadequate waste disposal practices.

The effect of autochthonous metal ions and humic substances on landfill leachate ozonation: Reactive oxygen species and 3D fluorescence analysis

This study investigated the ozonation process for landfill leachate treatment, focusing on the impact of autochthonous metal ions (Fe3+, Cu2+, Mn2+, Mg2+) and humic substances on the degradation of pollutants. Our findings revealed that the presence of these metal ions significantly enhanced the generation of hydroxyl radicals (OH), especially for Fe3+, demonstrating the highest catalytic activity. However, the humic substance attenuated the signals of reactive oxygen species (ROS), particularly the superoxide radical (O2−) in the presence of Cu2+ and Mn2+. These suggested that metal ions could complex with humic substances and further inhibit ROS production. Despite this inhibition, Fe3+ maintained its ROS-generating capacity even in the presence of humic substances, indicating its potential as a robust catalyst in landfill leachate. Batch tests further demonstrated that Fe3+ had the most positive effect on the degradation of sodium humate, as evidenced by a significant reduction in fluorescence regional integration (FRI) values. An integrated membrane bioreactor (MBR) and ozonation system were conducted, which firstly processed activated sludge treatment in the MBR and then pumped into the ozonation system via a peristaltic pump. After ozonation, the leachate overflowed back into the MBR for further treatment, creating a closed-loop system. It was observed that there was a significant reduction in humic substances, with a decrease of 96.31% in the FRI. Parallel factor analysis (PARAFAC) results corroborated these findings, which indicated a marked reduction in the abundance of humic-like substances C-BO-1 and C-BO-4 during the 17 days treatment period. This study revealed the pivotal role of microorganisms, ROS, metal ions, and humic substances in the treatment of landfill leachate. The metal ions presented in the leachate were confirmed to produce ROS during the ozonation process. However, a significant quantity of humic-like substances reduced the reactive ROS through complexation and consumption reactions. Simultaneously, microorganisms in the MBR system consumed humic substances such as C-BO-1 and C-BO-4, which promoted the degradation of contaminants in the landfill leachate. These insights provided implications for optimizing ozonation processes in leachate management, potentially leading to more efficient and environmentally sustainable waste disposal practices.