Pesticide residues in agricultural environments pose significant threats to aquatic ecosystems and human health. Most studies investigate the quantity, environmental, and risk assessment of pesticides in agricultural landscapes. However, farmers’ pesticide use and their knowledge on aquatic ecosystem contamination remain limited and poorly documented. This study evaluated pesticide residue concerns in agricultural settings, focusing on farmers’ uses and knowledge. It addressed pesticide application methods and their effects on aquatic ecosystems and human health through fish consumption. A cross-sectional design was employed and 300 farmers were selected using a multi-stage sampling technique from two agricultural districts – Savelugu Municipal and Tamale Metropolis. The study revealed that maize was most cultivated (29.6 %) with extensive pesticide treatment, particularly herbicides in the study setting. Findings revealed pesticide overuse (22.3 %), mixing of pesticides at the water bodies (24.7 %), improper leftover pesticide disposal methods (39.0 %) and inadequate storage practices (63.0 %) among farmers. About 21.3 % and 74.0 % of farmers have insufficient knowledge of the resulting consequences and the discharge of pesticides into the aquatic environment, respectively. Though the farmers are aware of the detrimental impact of pesticides on human health, there is still a lack of comprehension regarding the indirect consequences for aquatic ecosystems and non-target species. In the study setting, extension services may be important sources of knowledge. Thus, emphasizes the significance of improving extension programmes and utilizing peer-to-peer communication channels to support the adoption of best practices in pesticide management such as licenced high-temperature incinerators and cement kilns with sufficient emission controls. Focused training and outreach programmes designed to enhance farmers' knowledge and implementation of pesticide management plans are urgently needed. These interventions would help in reducing the dangers of pollution to aquatic ecosystems and human health.
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