Disposable Device Research Articles

Survey of Bariatric Surgeon Calibration Tube Use in Sleeve Gastrectomy.

The study objective was to identify variations in calibration tube use during sleeve gastrectomy and understand elements of ideal calibration tube performance. A survey was distributed via email and social media to bariatric surgeons, who were asked to rate the performance of their current calibration tube and an ideal calibration tube for five technical milestones involving visualization, positioning, suction capability, and sleeve sizing. Data was analyzed using descriptive and bivariate analyses including chi-square, Mann-Whitney, and Kruskal-Wallis tests. After eliminating incorrect screening question responses, 552 of 585 responses were analyzed. Demographics included 82% practicing in the USA, 64% in community practice, performing an average of 114 (SD = 94.5) sleeves annually. Calibration tubes used were most commonly reusable (54.2%) versus disposable (27.4%), endoscope (5.3%), or other (13.2%). Ninety-six percent of calibration tube sizes were 32 to 44 French. The reusable calibration tube had the lowest mean scores in all five technical areas, while the endoscope performed the best. When comparing surgeon current to ideal calibration tube performance, the endoscope performed closest to an ideal device, with significant p-values for technical milestones (i-iv). Despite the endoscope having the highest ratings for technical performance, surgeons rated higher satisfaction using disposable calibration tubes. Significant variation exists among the size and type of calibration tubes used for sleeve gastrectomy. Calibration tubes incorporating the visualization of an endoscope with the workflow of a disposable device could bridge technical performance and satisfaction goals for surgeons.

Simultaneous determination of ethylene oxide, 2-chloroethanol and ethylene glycol residues in medical device products by gas chromatography

A gas chromatography-based method was developed for the simultaneous and rapid determination of ethylene oxide (EO), 2-chloroethanol (ECH), and ethylene glycol (EG) residues in medical devices after EO sterilization. A sample weighing 2.5 g was added with 5 mL of ethanol as the extraction medium, and the residual substances in the sample were extracted at 40 ℃ for 4 h. The samples were separated on a DB-WAX capillary column (30 m×0.53 mm×1.0 μm) and determined using a hydrogen flame ionization detector. The temperature was maintained at 40 ℃ for 5 min, increased to 120 ℃ at a rate of 40 ℃/min, held for 5 min, and then increased to 200 ℃ at a rate of 6 ℃/min, held for 2 min. The flow rate of the nitrogen gas was 3 mL/min. The split ratio was 5∶1. The inlet and detector temperatures were 200 and 300 ℃, respectively. The changes in the chromatographic peak areas over time (0.5-10 h) under different temperatures (20, 30, 40, and 50 ℃) were investigated, and the optimal extraction condition was determined to be 40 ℃ for 4 h. In the experiments, quantification was performed using an external standard method. EO, ECH, and EG exhibited good peak shapes and separation effects as well as good linearity within their respective ranges. The linear correlation coefficients for EO, ECH, and EG were greater than 0.99. The limits of detection (LODs) for EO, ECH, and EG were in the range of 0.10-0.40 μg/g, and the limits of quantification (LOQs) were in the range of 0.30-1.20 μg/g. The average recoveries under different spiked levels were in the range of 91.08%-116.08%, and the relative standard deviations (n=6) were in the range of 0.56%-8.45%. EO, ECH, and EG residues were found to exist at different levels in the medical devices tested. In particular, disposable infusion sets must be paid careful attention. ECH and EG were not detected in disposable sterile medical devices made of non-polyvinyl chloride materials, which may be due to the fact that the products themselves did not contain chloride ions, they were not exposed to chlorine-containing substances during their production, sterilization, storage, transportation, use, etc. This study established a method to detect EO residues in disposable medical devices, and has the advantages of simple operation, excellent specificity, accurate quantification, and good reproducibility. It can simultaneously detect three residual substances in medical devices while meeting the actual detection requirements for EO, ECH, and EG residues. The method can be used to scientifically and effectively evaluate the risk of EO residues in single-use medical devices sterilized with EO, and will be helpful for improving the quality of medical devices, ensuring the safety of device use, and providing a reference for regulatory supervision and testing.

Simple and disposable device based on gold nanoparticles modified screen-printed carbon electrode for detection of ciprofloxacin

Simple and disposable device based on gold nanoparticles modified screen-printed carbon electrode for detection of ciprofloxacin

Disposable circumcision suture device: A potential bedside tool for circumcision – A series of 18 cases

Male circumcision (MC) is a well-established surgical procedure with a long history. It offers various health benefits, including protection against infections and diseases. The disposable circumcision suture device (DCSD) helps in faster wound healing which will result in reduced complications compared to other conventional circumcision methods. In this report, we share our experience with the stapler method in MC for various dermatological indications. The study emphasized the significance of circumcision in dermatological applications and its potential impact on clinical practice.

Marketing for Sensa: A novel "zero nicotine vapor product" from a major tobacco company.

On July 1, 2024, RJ Reynolds Vapor Company (RJRVC) introduced Sensa, the first disposable "zero nicotine vapor product" from a major tobacco company. Sensa sold more than 100,000 units as of September 10, 2024 and has been marketed through several channels. Direct-mail and magazine ads appeal to consumers' senses (e.g., "awaken your senses") and showcase Sensa's six flavor options: Berry Fusion, Berry Watermelon Fusion, Blueberry Frost, Mint Frost, Passionfruit Frost, and Watermelon Frost. Our chemical characterization of all Sensa flavors (except Berry Watermelon Fusion) found neotame (a potent artificial sweetener) and WS-23 (a synthetic coolant) in all samples, while all "Frost" flavors tested contained another synthetic coolant, WS-3. Although RJRVC states that Sensa is for "adult tobacco and vapor consumers," Sensa's resemblance to popular disposable vaping devices and youth-appealing flavors raise concerns about Sensa's appeal to young people, and limited regulations for "zero nicotine" products introduce uncertainty about product safety.

Single-Use Technology in Purification of Monoclonal Antibiotics

The advent of Single-Use Technologies (SUT) has revolutionized biopharmaceutical manufacturing, offering a flexible and efficient alternative to traditional methods. This paper explores the diverse applications of SUT, focusing on therapeutic plasma filtration, clarifying processes, virus removal strategies, and chromatography solutions. Highlighting the advantages such as convenience, reduced contamination risk, and streamlined processes, the document delves into the economic and environmental impacts of adopting SUT. Despite challenges in scalability and automation, specific examples like Immusorba PH-350 and Pall Biotech's AllegroTM underscore the significant adsorption efficiency achieved with single-use systems. Anticoagulation strategies tailored for single-use plasma filtration contribute to optimal systemic performance. The dynamic market for disposable devices, featuring prominent manufacturers like Fresenius HemoCare, Kaneka, Medicap Clinic GmbH, Miltenyi Biotec, Toray Medical, and Biotex, reflects the widespread adoption of SUT across various medical applications. The ongoing innovation in overcoming scalability limitations and the commitment to standardization position SUT as a transformative force in modern biopharmaceutical manufacturing. In conclusion, the economic implications and positive environmental impact underscore SUT's potential to shape the future of the industry. Keywords: Single Use Technology, Reduced Contamination, Antibiotics, Biopharmaceutical, Good Manufacturing Practices, Mixing bag, Aseptic Connections, Single use Bioreactors, Disposable Chromatography Columns, Single use Filtration, Running Cost, Labour charges

Urban Organic Nutrients Harmless Treatment System

To solve the common problem of pet droppings in urban parks and improve the conflict caused by raising pets in the community, a pet droppings collection and disposal device was designed to be environmentally friendly and aesthetically pleasing. This device allows for protecting animal rights while turning waste into useful organic fertilizer that can used for community and park fertilization. This is not only good for plant growth but also can reduce the use of chemical fertilizers and reduce environmental pollution.

Abstract B017: Kick Vaping: Feasibility, acceptability, and preliminary impact of a vaping cessation text messaging intervention for Latino young adults

Abstract Objective: The purpose of this study was to assess the feasibility, acceptability, and preliminary impact of Kick Vaping among Latino young adults. Methods: Forty Latino young adults (ages 18 to 25) who were currently vaping received Kick Vaping, a vaping cessation text messaging intervention available in English and Spanish. Feasibility was measured by the eligibility rate (defined as the proportion of individuals screened who were eligible), enrollment rate (the proportion of eligible individuals who enrolled), and follow-up rate (the proportion of participants who completed the follow-up assessment at Month 3). Acceptability was measured by overall satisfaction with the intervention and perceived helpfulness. Preliminary impact was measured by self-reported 7-day point prevalence abstinence (not vaping, even a single puff, in the past seven days) and changes in self-efficacy. Results: A total of 203 individuals were identified, 61 completed the eligibility assessment, and 55 were eligible to participate in the study. Forty individuals consented to participate in the study and enrolled in Kick Vaping. The eligibility and enrollment rates were 30.0% (61/203) and 72.7% (40/55), respectively. At baseline, participants’ mean age was 22.3 years old (SD 2.1), 45.0% of participants were female, and 45.0% were heterosexual or straight. Half of the participants had a high school education or less (50.0%) and were currently employed. Fourteen (35.0%) participants reported speaking English and Spanish equally. Most participants used disposable devices (70%), vaped daily (97.5%), had low (37.5%) or medium (35.0%) e-cigarette dependence, and had attempted to quit in the past year (72.5%). At Month 3, the follow-up rate was 90% (36/40). Treating those lost to follow-up as participants who continued vaping, 75% (30/40) of participants self-reported 7-day point prevalence abstinence. Self-efficacy mean scores significantly increased from 30.65 (SD 8.07) at baseline to 50.11 (SD 10.57) at follow-up (p<0.01). Moreover, most participants (88.9%, 32/36) reported being satisfied/extremely satisfied with Kick Vaping. Additionally, most participants (83.3%, 30/36) reported that the intervention included “just the right amount of messages”. Lastly, most participants (86.1%, 31/16) reported that Kick Vaping helped them quit vaping “a lot”. Conclusion: It is feasible to recruit and retain Latino young adults in a vaping cessation text messaging intervention. Moreover, Kick Vaping generated high satisfaction and perceived helpfulness among Latino young adults, significantly increased self-efficacy, and resulted in a notable vaping cessation rate at Month 3. Additional testing in a randomized clinical trial is warranted to assess the efficacy of the intervention. Citation Format: Rafael H. Orfin, Jeffrey Wilmer Ramos Santiago, Ritsel Decena Soriano, Eliany Romero Acosta, Diana Bermudez, Yaneth L. Rodriguez, Dongmei Li, Irfan Rahman, Scott McIntosh, Deborah J. Ossip, Ana Paula Cupertino, Francisco Cartujano-Barrera. Kick Vaping: Feasibility, acceptability, and preliminary impact of a vaping cessation text messaging intervention for Latino young adults [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr B017.

Clinical observation of low temperature plasma ablation combined with collagenase injection in lumbar disc herniation.

To observe the effect of low temperature plasma ablation combined with collagenase injection on lumbar disc herniation. 90 patients with lumbar disc herniation admitted to the pain department of our hospital and receiving surgical treatment from April 2021 to April 2023 were included in this retrospective study, and were divided into 2 groups according to different treatment plans. One group was treated with low-temperature plasma ablation combined with collagenase injection, and the other group was treated with low-temperature plasma ablation alone. The sample size of both groups was 80 cases. Peripheral blood was collected by fasting in the morning at 5 time points before surgery, 1, 3, 7 and 14 days after surgery, and 5 mL of whole venous blood was collected by disposable vacuum blood collection device. Serum levels of pro-inflammatory factor interleukin (IL)-1α, IL-1β, IL-6, IL-8, tumor necrosis factor-α and anti-inflammatory factor IL-4, IL-10 were detected by ELISA. VAS scores were used to evaluate postoperative low back pain. ODI and Lehmann Lumbar Function Scale were used to evaluate postoperative lumbar function. The contents of IL-1α, IL-1β, IL-6, IL-8, tumor necrosis factor-α and anti-inflammatory factor IL-4, IL-10 in the cryo-plasma ablation combined with collagenase injection group were significantly lower than those in the cryo-plasma ablation group alone (P < .05). The VAS score of cryo-plasma ablation combined with collagenase injection group was significantly lower than that of cryo-plasma ablation group at 1 day and 3 months after treatment, and the difference was statistically significant (P < .05). The ODI score of cryo-plasma ablation combined with collagenase injection group was significantly lower than that of cryo-plasma ablation group at 1 day and 3 months after treatment, and the difference was statistically significant (P < .05). The Lehmann score of cryo-plasma ablation combined with collagenase injection group was significantly higher than that of cryo-plasma ablation group at 1 day and 3 months after treatment, and the difference was statistically significant (P < .05). The overall efficacy of low-temperature plasma ablation combined with collagenase injection is better than that of low-temperature plasma ablation alone. Low temperature plasma ablation combined with collagenase injection in the treatment of patients with lumbar disc herniation has less pain, faster recovery.

Content analysis of conversations on Reddit: reactions to FDA’s ENDS prioritized enforcement policy

IntroductionOn January 2, 2020, the FDA announced a policy focused in part on prioritizing enforcement of flavored (other than tobacco- or menthol-flavored) cartridge-based electronic nicotine delivery systems (ENDS) without premarket authorization.MethodsWe used a query to identify Reddit conversations relevant to the policy from January 2 to May 6, 2020. Our sample included 576 posts (46 posts and 530 accompanying comments). Two analysts coded posts for mentions of use behaviors (e.g., switching, quitting), purchasing behaviors (e.g., purchasing from retailer new to the user), and flavored products. We summarized frequencies of coded data and provided illustrative quotes.ResultsOnly 21.0% (121/576) of posts mentioned use behavior. Switching behavior was the most common use behavior mentioned (50.4%, 61/121). Most switching behavior posts focused on ENDS-related switching (91.8%, 56/61). The most common ENDS-related switching behaviors mentioned were switching to an open tank (45.9%, 28/61) or device with refillable pods/cartridges (44.3%, 27/61); 8.2% (5/61) mentioned switching to disposables. Just 15.5% (89/576) of posts mentioned purchasing behavior, with the most common being purchasing from a retailer new to the user (32.6%, 29/89). Only 6.8% (39/576) of posts mentioned specific flavors.ConclusionReddit posts about the policy commonly discussed switching to non-cartridge-based ENDS products, such as open tank systems or disposable devices, and purchasing products from different online sources that were still selling these products. Findings suggest that publicly available Reddit data can complement data from traditional sources (e.g., surveys, sales) to understand potential unintended consequences associated with policies by exploring the public’s reactions.

Policy pathways to sustainable E-waste management: A global review

Electronic waste (E-waste) is a critical challenge of today's period, with around 57 million tons generated in 2021, of which only about 9 million tons were properly recycled, E-waste tends to affect the globe with issue like, illegal recycling, improper landfill disposal, illicit exports and others. Addressing this issue requires sustained, well-structured efforts, with effective policy interventions which are key towards reducing E-waste, by providing a framework for minimizing its generation and safeguarding the environment. These policies can ensure proper disposal methods, public awareness, device repair and refurbishment, and recycling rates with E-waste management monetizing the efforts of E-waste management startups. The study examines innovative E-waste policies across various countries which includes Extended Producer Responsibility (EPR), WEEE directives, regional laws, and State level laws, highlighting successful strategies such as incentivized recycling programs and stricter regulations on hazardous materials. The aim of the paper is to update information with an emphasis on global trends of E-waste, emphasizing the importance of robust policies in reducing environmental harm and lowering the cost of new electronics through efficient recycling.

Investigating the sterile surgical supply waste in laparotomy surgery

BackgroundOperating rooms contribute to over 40% of hospital expenses, with a portion attributed to waste from single-use, sterile surgical supplies (SUSSS). This research aimed to determine the amount of cost wastage due to not using SUSSS during laparotomy procedures.MethodsA descriptive-analytical investigation was conducted in two prominent teaching hospitals in Mashhad, Iran 2018. Seventy-seven laparotomy surgeries were scrutinized, documenting both used and unused disposable devices, with their respective costs being assessed. Data analysis was performed using SPSS version 16 software.ResultsThe study revealed that during surgery in the operating rooms, waste of SUSSS averaged 5.9%. Betadine solution and sterile Gauze types were the top two contributors to resource wastage. Sterile Gauze types incurred the highest cost loss. The study found a significant correlation between cost wastage and surgeon experience (r = 0.296, P < 0.001) as well as surgery duration (r = 0.439, P < 0.001).ConclusionInadequate management of available and commonly used disposable supplies leads to increased hospital expenses. Enhancing the surgical team’s knowledge of sterile surgical supplies usage and making thoughtful selections can play a vital role in curbing health costs by minimizing waste of SUSSS in the operating rooms.

Kick Vaping: Feasibility, acceptability, and preliminary impact of a vaping cessation text messaging intervention for Latino young adults

Objective To assess the feasibility, acceptability, and preliminary impact of Kick Vaping among Latino young adults. Methods Forty Latino young adults (ages 18 to 25) who were currently vaping received Kick Vaping, a vaping cessation text messaging intervention available in English and Spanish. Feasibility was measured by the eligibility, enrollment, and follow-up rates. Acceptability was measured by overall satisfaction with the intervention. Preliminary impact was measured by self-reported 7-day point prevalence abstinence and changes in self-efficacy. Results Two hundred three individuals were identified, 61 were assessed for eligibility, and 55 were eligible. Forty individuals consented to participate and were enrolled in Kick Vaping. At baseline, most participants used disposable devices (70%), vaped daily (97.5%), had low (37.5%) or medium (35.0%) e-cigarette dependence, and had attempted to quit in the past year (72.5%). At Month 3, the follow-up rate was 90% (36/40). Treating those lost to follow-up as participants who continued vaping, 75% (30/40) of participants self-reported 7-day point prevalence abstinence. Self-efficacy mean scores significantly increased from 30.65 (SD 8.07) at baseline to 50.11 (SD 10.57) at follow-up (p < 0.01). Most participants (88.9%, 32/36) reported being satisfied/extremely satisfied with Kick Vaping. Conclusion It is feasible to recruit and retain Latino young adults in a vaping cessation text messaging intervention. Kick Vaping generated high satisfaction among Latino young adults, significantly increased self-efficacy, and resulted in a notable vaping cessation rate at Month 3. Additional testing in a randomized controlled trial is warranted to assess the efficacy of the intervention.

Validation of the Microlab Salmonella for Detection of Salmonella O Group A-E in Selected Foods: AOAC Performance Tested MethodSM 052401.

Microlab Salmonella is an all-in-one solution for detection of Salmonella O Groups A-E that integrates, in a single disposable device, all elements to perform the analysis; a ready-to-use enrichment broth, a lateral flow immunochromatography test and a chemical agent for bacterial inactivation. Microlab Salmonella can be easily used on-site and does not require specific laboratory equipment or technical skills. The device is sealed during the analysis avoiding risks of contamination and is therefore safe to use in food production environments. This report details the method validation study for raw ground beef, raw turkey (thermal processed, marinated), fresh cheese, deli ham and pasteurized liquid egg. Matrix studies and inclusivity/exclusivity, product consistency/stability, and robustness testing were conducted to assess the method's performance. Inclusivity/exclusivity testing showed that the Microlab Salmonella method was able to detect Salmonella O Group A-E isolates while excluding the non-Salmonella strains. In the matrix studies the differences between the candidate and reference methods were not statistically significant. Minor variations in test parameters (enrichment time, time to read results, and enrichment temperature) did not affect the performance of the Microlab assay. There were no statistically significant differences between recently manufactured lots and those that were halfway through their expiration period or between those that were close to expiring. The reported data confirms that Microlab Salmonella is reliable for detecting Salmonella in raw ground beef, raw turkey (thermal processed, marinated), fresh cheese, pasteurized liquid egg and deli ham. Microlab Salmonella is validated to detect Salmonella in select food matrixes. Microlab Salmonella integrates the steps of enrichment, detection, and inactivation in a single device.

Cost-effective fully 3D-printed on-drop electrochemical sensor based on carbon black/polylactic acid: a comparative study with screen-printed sensors in food analysis.

3D-printing technology allows scientist to fabricate easily electrochemical sensors. Until now, these sensors were designed employing a large amount of material, which increases the cost and decreases manufacturing throughput. In this work, a low-cost 3D-printed on-drop electrochemical sensor (3D-PES) was fully manufactured by fused filament fabrication, minimizing the number of printing layers. Carbon black/polylactic acid filament was employed, and the design and several printing parameters were optimized to yield the maximum electroanalytical performance using the minimal amount ofmaterial. Print speed and extrusion width showed a critical influence on the electroanalytical performance of 3D-PES. Under optimized conditions, the fabrication procedure offered excellent reproducibility (RSD 1.3% in working electrode diameter), speed (< 3min/unit), and costs (< 0.01 $ in materialcost). The 3D-PES was successfully applied to the determination of phloridzin in apple juice. The analytical performance of 3D-PES was compared with an equivalent commercial on-drop screen-printed electrode, yielding similar precision and accuracy but lower sensitivity. However, 3D-PES provides interesting features such as recyclability, biodegradability, low-cost, and the possibility of being manufactured near the point of need, some of which meets several demands of Green Chemistry. This cost-effective printing approach is a green and promising alternative for manufacturing disposable and portable electroanalytical devices, opening new possibilities not only in on-site food analysis but also in point-of-care testing.

Formative and validation human factors studies of a new disposable prefilled injection device for subcutaneous delivery of acthar gel (repository corticotropin injection)

ABSTRACT Background The administration of repository corticotropin injection (Acthar Gel) via a single-dose prefilled injector (SelfJect) is intended to provide a simple, ergonomic alternative to traditional injection. Iterative human factors (HF) studies were conducted to identify potential use deviations and ensure appropriate device use. Research design and methods This article presents seven formative studies, a validation study (with prior pilot validation studies), and a supplemental validation study with participants including lay users, patients, caregivers, and healthcare providers. Participant interactions with SelfJect and the user interface were assessed. Use deviations, user preferences, and participants’ ability to successfully complete tasks were evaluated to generate modifications to the device and user interface. Results In the validation study, 91% of participants successfully administered their first injection. Use errors were rare with simulated-use (6.9%) and knowledge-based (1.6%) testing. Use deviations were commonly attributed to experimental artifact or information oversight, and device warming had the most use errors (49% of participants), even with extensive testing and adjustments to the user interface. Conclusions SelfJect was able to be used in a safe and effective manner by the intended users. Iterative HF studies informed the mitigation of use-related risks to reduce the occurrence of use deviations during simulated use.

Assessment of bisphenol accumulation from disposable devices used sequentially in IVF routine procedures

Research question: To investigate the release of bisphenols (BPs) from disposable devices (DDs) used in ART procedures and to determine whether they accumulate when several DDs are used sequentially under routine conditions.Design: A comprehensive assessment of 19 individual DDs (31 measurements) and 9 combinations of DDs replicating the main steps in an ART procedure was undertaken. The extraction of BPs followed routine use conditions (temperature and duration). The concentration of 10 BPs was determined using on-line solid phase extraction/liquid chromatography/mass spectrometry methodology.Results: Bisphenol S (BPS) was quantified consistently from 100 mm culture dishes (32 ± 20 pg) and from high security sperm straws (3 ± 1 pg). Also, BPS and Bisphenol A (BPA) were quantified consistently from spermicide-free condoms (95 ± 78 and 83 ± 49 pg, respectively). No other BPs were detected in individually tested DD.When DDs were used together, BPA and BPS were both detected consistently in the association of 13 DDs mimickin;g the sperm collection in condom and its preparation (46 ± 16 and 43 ± 32 pg, respectively). BPS was quantified consistently in the association of 14 DDs mimicking the sperm collection, its preparation and freezing (10 ± 4 pg), and in the association of 17 DDs mimicking the oocyte pick-up (37 ± 22 pg).Conclusions: BPA and BPS are released in small quantities from some DDs used in routine conditions during ART procedures, but do not clearly accumulate when these consumables are used together.

Green endoscopy, one step toward a sustainable future: Literature review.

Rapid climate change or climate crisis is one of the most serious emergencies of the 21st century, accounting for highly impactful and irreversible changes worldwide. Climate crisis can also affect the epidemiology and disease burden of gastrointestinal diseases because they have a connection with environmental factors and nutrition. Gastrointestinal endoscopy is a highly intensive procedure with a significant contribution to greenhouse gas (GHG) emissions. Moreover, endoscopy is the third highest generator of waste in healthcare facilities with significant contributions to carbon footprint. The main sources of direct carbon emission in endoscopy are use of high-powered consumption devices (e.g. computers, anesthesia machines, wash machines for reprocessing, scope processors, and lighting) and waste production derived mainly from use of disposable devices. Indirect sources of emissions are those derived from heating and cooling of facilities, processing of histological samples, and transportation of patients and materials. Consequently, sustainable endoscopy and climate change have been the focus of discussions between endoscopy providers and professional societies with the aim of taking action to reduce environmental impact. The term "green endoscopy" refers to the practice of gastroenterology that aims to raise awareness, assess, and reduce endoscopy´s environmental impact. Nevertheless, while awareness has been growing, guidance about practical interventions to reduce the carbon footprint of gastrointestinal endoscopy are lacking. This review aims to summarize current data regarding the impact of endoscopy on GHG emissions and possible strategies to mitigate this phenomenon. Further, we aim to promote the evolution of a more sustainable "green endoscopy".

Paper-Based Microfluidic Analytical Device Patterned by Label Printer for Point-of-Care Blood Glucose and Hematocrit Detection Using 3D-Printed Smartphone Cassette.

This study presents a portable, low-cost, point-of-care (POC) system for the simultaneous detection of blood glucose and hematocrit. The system consists of a disposable origami microfluidic paper-based analytical device (μPAD) for plasma separation, filtration, and reaction functions and a 3D-printed cassette for hematocrit and blood glucose detection using a smartphone. The origami μPAD is patterned using a cost-effective label printing technique instead of the conventional wax printing method. The 3D-printed cassette incorporates an array of LED lights, which mitigates the effects of intensity variations in the ambient light and hence improves the accuracy of the blood glucose and hematocrit concentration measurements. The hematocrit concentration is determined quantitatively by measuring the distance of plasma wicking along the upper layer of the origami μPAD, which is pretreated with sodium chloride and Tween 20 to induce dehydration and aggregation of the red blood cells. The filtered plasma also penetrates to the lower layer of the origami μPAD, where it reacts with embedded colorimetric assay reagents to produce a yellowish-brown complex. A color image of the reaction complex is captured using a smartphone inserted into the 3D-printed cassette. The image is analyzed using self-written RGB software to quantify the blood glucose concentration. The calibration results indicate that the proposed detection platform provides an accurate assessment of the blood glucose level over the range of 45-630 mg/dL (R2 = 0.9958). The practical feasibility of the proposed platform is demonstrated by measuring the blood glucose and hematocrit concentrations in 13 human whole blood samples. Taking the measurements obtained from commercial glucose and hematocrit meters as a benchmark, the proposed system has a differential of no more than 6.4% for blood glucose detection and 9.1% for hematocrit detection. Overall, the results confirm that the proposed μPAD is a promising solution for cost-effective and reliable POC health monitoring.

Development of an Integrated Disposable Device for SARSCoV-2 Nucleic Acid Extraction and Detection

ObjectiveTo develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). MethodsWe designed, developed, and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection. The precision of the liquid transfer and temperature control was tested. A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). The entire process, from SARS-CoV-2 nucleic acid extraction to amplification, was evaluated. ResultsThe precision of the syringe transfer volume was 19.2 ± 1.9 μL (set value was 20), 32.2 ± 1.6 (set value was 30), and 57.2 ± 3.5 (set value was 60). Temperature control in the amplification tube was measured at 60.0 ± 0.0 °C (set value was 60) and 95.1 ± 0.2 °C (set value was 95) respectively. SARS-Cov- 2 nucleic acid extraction yield through the device was 7.10 × 106 copies/mL, while a commercial kit yielded 2.98 × 106 copies/mL. The mean time to complete the entire assay, from SARS-CoV-2 nucleic acid extraction to amplification detection, was 36 min and 45 s. The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL. ConclusionThe integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test (POCT).