Dispersion Of Microplastics Research Articles

Assessment of microplastic characterization and distribution from surface water and the seabed in the Flores Sea, Indonesia

Despite growing global concerns, there has been limited research on the characterization and distribution of microplastics in the Indonesian Throughflow (ITF) pathways, such as the Flores Sea. The Flores Sea is a component of the Indian–Pacific Current, a significant ocean current system that links the Pacific and Indian oceans and has the capacity to carry pollution over large marine ecosystems, making this research extremely important. Understanding the microplastic pollution in this area informs local environmental management. It provides insights into how these currents may distribute microplastics (MPs) across regional and global scales, impacting marine life and human health far beyond the immediate area. To respond to these concerns, this research aims to determine the characterization and distribution of MPs at six research sites in the Flores Sea that are precisely located within the ITF. This work exhibits an extensive dataset focusing on the occurrence, attributes, and dispersion of microplastics in the Flores Sea. The water sampling was carried out during a Jala Citra 3 by the Indonesian Navy from April to May 2023. Sea surface water samples were collected using a Neuston net, while sediment samples were taken from three stations at the shallowest depth using the Ekman Grab sampler. Additionally, abundance, size, shape, and color analyses were conducted using a light microscope, and microplastic types were identified through Raman spectroscopy. The results indicated that the Flores Sea waters and sediment are polluted with microplastics, with relative abundances ranging from 0.75 ± 0.49 to 2.13 ± 0.25 items/l samples. The most dominant shapes identified were filament (77.45%) and fragment (13.40%), with sizes varying between surface water 4.70 to 3799.25 μm and seabed from 67.20 mm to 2176.87 mm, while black (30.07%) and blue (24.51%) were reported as the common MPs colors. The identified polymers include PET and PE. This study confirms visual evidence of microplastics in the open waters of eastern Indonesia. While it may not fully capture the wide range of temporal variations, it establishes initial microplastic presence and dispersion levels. Given that the ITF influences both the Pacific and Indian Oceans, this research contributes to the global understanding of microplastic distribution across ocean basins, underscoring the need for coordinated international efforts to address marine pollution.

From source to sink: part 2-seasonal dispersion of microplastics discharged in the NW Mediterranean Sea by the Rhone River in southern France.

As the largest individual contributor of freshwater inflow to the basin, the Rhone River is likely to be one of the main sources of microplastics (MPs) to the Mediterranean Sea. In order to predict the fate of MPs discharged by the Rhone River, an innovative 3D Lagrangian dispersion of its particles associated with vertical velocities was modeled in Mediterranean ocean currents. Through winter and summer scenarios, the seasonal variability of transfers and the corresponding accumulation areas were depicted in the Northwestern Basin according to hydrodynamic conditions on the continental shelf of the Gulf of Lion and to the frontal dynamics from the Pyrenees to the North Balearic fronts. Our results indicated that MP transfers were driven by mesoscale and sub-mesoscale structures, resulting in steep concentration gradients across fronts during summer, while winter energetic mixing favored a more efficient and homogeneous spreading. After a year of drift, high MP retention (up to 50%) occurred in the coastal zone of the Gulf of Lion near the river mouth, with a large contribution of sinking MPs and an increase in stranding during the highest freshwater inflows of the winter season. Conversely, up to 60% of the floating MPs were exported to the Algerian Basin and then to the Eastern Mediterranean. This west-to-east transfer led to significant stranding on the islands, prevailing on the northern coasts of the Balearic Islands in winter (6% of floating inputs) and on the western coasts of Corsica and Sardinia in summer (13%). The southern Mediterranean coasts, from Algeria to Tunisia, represented also a major sink for floating debris with stranding ranging from 9 to 35% of MPs discharged in winter and in summer, respectively. We estimated that 3.5 to 5 t of the Rhone MPs remained in the surface layer at the end of the year, with high concentrations in the Ionian Sea.

Field assessment of engineered bioretention as microplastics sink through site characterization and hydrologic modeling

Green stormwater infrastructure (GSI) practices like bioretention are considered a sink for microplastics washed in from urbanized land uses-land covers, and thereby regulating the environmental dispersion of microplastics. However, the capacity of GSI in microplastic sequestration remain unclear. This work investigated the spatial distributions of microplastics within bioretention cells and their soils, concentration in the GSI groundwater monitoring well, and the overall potential of GSI as a sink for microplastics. The average microplastic concentration in GSI soils (2718 - 3833 MPs/kg dry soil) is 7−10 times higher than background soils (262 - 520 MPs/kg dry soil). The most abundant microplastics observed were polypropylene (57 %), polyethylene (17 %), and rubber/bituminous (16 %), indicating the major sources of microplastics derived from post-consumer plastics and tire wear. Soils closest to the GSI stormwater inlet sequestered the most particles, and the microplastic concentration decreased with soil depth. Our hydrologic modeling, based on measured soil and hydraulic parameters, suggested that runoff overtopping the freeboard of the bioretention cell – and potentially dispersing microplastics into the surrounding environment – was unlikely. Microplastics in the GSI groundwater monitoring well was minimal at 11 MPs/L. These results suggest that GSI can capture and sequester microplastics to mitigate microplastic pollution in the environment.

Plasmonic array at liquid-liquid interface for trace microplastics detection

Microplastics (MPs) pollution has become a focal point of global concern, as the widespread distribution of MPs in the environment poses a potential threat to ecosystems and human health. Despite surface-enhanced Raman scattering (SERS) being one of the most promising detection techniques, the “coffee ring effect” of MPs and their unique size scale make effective contact with SERS substrates challenging. Here, we propose a novel strategy for MPs detection by constructing a plasmonic metasurface at the liquid-liquid interface (LLI). The fluidic properties of LLI provide more flexibility for the uniform dispersion of MPs and the assembly of plasmonic arrays. MPs are captured and assembled on an atomic-level metasurface in a core-shell structure (AuNPs@MPs), providing abundant “hot spots” and additional volume-enhanced Raman effects. This results in enhanced electromagnetic field around MPs and increased enhancement factors, demonstrating high SERS sensitivity and uniformity. This sensing platform provides a new approach for trace detection of MPs and has been validated in practical matrix.

Full Rotational Dynamics of Plastic Microfibers in Turbulence.

The motion, settling, and dispersion of microplastics in the ocean are determined by their rotational dynamics. We present experiments on elongated, large aspect ratio, and mildly curved plastic fibers slightly longer than the Kolmogorov length scale. Exploiting their uniquely identifiable three-dimensional orientation, we perform original optical Lagrangian investigations and provide a set of homogeneous data on their rotation rates around their longitudinal axis: spinning rate, and transversal axes: tumbling rates, which we explain in the context of the general features of turbulence.

Emerging pollutant in surface water bodies: a review on monitoring, analysis, mitigation measures and removal technologies of micro-plastics.

Water bodies play a crucial role in supporting life, maintaining the environment, and preserving the ecology for the people of India. However, in recent decades, human activities have led to various alterations in aquatic environments, resulting in environmental degradation through pollution. The safety of utilizing surface water sources for drinking and other purposes has come under intense scrutiny due to rapid population growth and industrial expansion. Surface water pollution due to micro-plastics (MPs) (plastics < 5mm in size) is one of the emerging pollutants in metropolitan cities of developing countries because of its utmost resilience and synthetic nature. Recent studies on the surface water bodies (river, pond, Lake etc.) portrait the correlation between the MPs level with different parameters of pollution such as specific conductivity, total phosphate, and biological oxygen demand. Fibers represent the predominant form of MPs discovered in surface water bodies, exhibiting fluctuations across seasons. Consequently, present study prioritizes understanding the adaptation, prevalence, attributes, fluctuations, and spatial dispersion of MPs in both sediment and surface water environments. Furthermore, the study aims to identify existing gaps in the current understanding and underscore opportunities for future investigation. From the present study, it has been reported that, the concentration of MPs in the range of 0.2-45.2 items/L at the Xisha Islands in the south China sea, whereas in India it was found in the range of 96 items/L in water samples and 259 items/kg in sediment samples. This would certainly assist the urban planners in achieving sustainable development goals to mitigate the increasing amount of emergent pollutant load.

Inter-annual variation in the microplastics abundance in feces of the Baird's tapir (Tapirus bairdii) from the Selva Maya, México

Microplastics (MPs) are found in a wide range of ecosystems, from the Arctic to the deep ocean. However, there is no data on their presence in terrestrial mammals that inhabit the Selva Maya. The aim of this study is to detect the presence of MPs in the feces of the Baird's tapir (Tapirus bairdii) from the region of Calakmul, located in the Yucatan Peninsula, Mexico. We analyzed 129 fecal samples collected during 2017 and 2018, obtaining 57 and 72 samples during the rainy and dry seasons respectively. Sixty-eight percent of the samples contained 743 MPs with a mean of 19.3 ± 28.1 MPs/kg of dry weight (DW) feces in both years. An inter-annual variation in the average abundance of microplastic was observed during the two-year period (2017–2018), with a 72 % increase in these plastic particles in feces. Fourteen polymers were identified, with ethylene vinyl acetate (EVA), polypropylene (PP) and polyester (PES) being the most abundant during both years. Although the effects of MPs on the health of tapirs are not known, their presence is cause for concern. There is an urgent need for the implementation of appropriate plastic waste management programs in communities of the Selva Maya to diminish the consumption of MPs in species including humans where they pose a significant risk to health. Environmental implicationsThe use of plastics worldwide is increasing every day, so the presence of microplastics is and will continue to be a major environmental problem. It is known that contaminants can adhere to plastics, making them hazardous materials. Microplastics can contaminate remote areas such as Biosphere Reserves. Terrestrial species such as the tapir can ingest microplastics, putting their health at risk. Knowing the dispersion of microplastics is very important in order to manage them properly, taking into account their emission sources and type of polymer.

CFD Analysis of Microplastic Transport over the Slopes

Microplastics, ubiquitous in our environment, are significantly impacted by the hydrodynamic conditions around them. This study utilizes CFD to explore how various breaker types influence the dispersion and accumulation of microplastics in nearshore areas. A special focus is given to the impact of wave dynamics and particle size, particularly on buoyant microplastics in spilling breakers. It was discovered that spilling breakers, common on gently sloping seabeds, encourage broad dispersion of microplastics, notably for smaller-sized particles. Plunging breakers exhibit a similar pattern but with less dispersion and an initial forward movement of neutral and heavy particles. Surging breakers feature minimal dispersion and a distinct oscillatory motion. It has been observed that medium-sized particles with a 1 mm diameter in this work exhibit the most substantial forward movement, likely due to an optimal balance between inertia and viscosity, enabling an effective response to wave momentum. Larger particles, influenced mainly by inertia, tend to show less dispersion and advection. Meanwhile, smaller particles, more affected by viscosity, demonstrate greater dispersion, interacting extensively with wave-induced turbulence. This study reveals the significance of inertia in the behavior of microplastics over slopes, emphasizing the importance of considering inertial effects for precise modeling of microplastic movement in nearshore areas.

Modelling the Potential Long-Range Dispersion of Atmospheric Microplastics Reaching a Remote Site

The study of the microplastic dispersion in the atmosphere is a relatively new field of research. In the studies available in literature, it was suggested that long-range transport may play a main role in the motion of microplastics towards remote sites, where no significant local sources are present. In this framework, here the Lagrangian particle dispersion model MILORD was used in the backward mode to investigate the potential long-range dispersion of microplastics in the atmosphere and to assess possible source areas that may determine microplastic pollution in a remote site. The chosen case study refers to the work of Allen et al. (2019), who analysed the observations of atmospheric microplastics deposition in a remote and pristine site in the French Pyrenees, also through numerical backward trajectories. Based on their dataset, they showed that microplastics could arrive at the site from distances up to about 100 km. Yet, they considered that it was unlikely to have only microplastic emissions from local sources, because of the low population density in the area. The results of MILORD simulations for different periods showed that for this case study the dominant scale of the microplastics transport process is regional up to mesoscale, while long-range transport may contribute when the tracer is mostly travelling in the free atmosphere before being deposited at the site. The relative contributions of dry and wet depositions and corresponding rates were also assessed. It was confirmed that plastic particles characterised by the highest settling velocities, reasonably associated to large particles, are unlikely to be subjected to long-range transport.

Occurrence of Microplastics in Kemena River and Niah River of Sarawak, Malaysia

Microplastics in freshwater have been identified as a significant contributor to plastic pollution in marine environments. However, the effect of urbanization on the quantity and spatial dispersion of microplastics in freshwater ecosystems of Sarawak and Malaysia remains unclear. The primary objectives of this study are to investigate the quantity and distribution of microplastics in water and riverbank sediments, as well as to analyze the properties of microplastic particles in the Kemena and Niah rivers. The selection of these rivers was based on the presence of commercial, residential, and industrial areas along their lengths. A total of 24 water and soil sediment samples were collected from three different sites along the Kemena and Niah rivers. The concentration of microplastics in water samples ranged from 60 to 128 items per liter, while sediment samples ranged from 46 to 76 items per liter. The sediment samples also contained microplastics ranging from 21 to 40 and 45 to 125 items per kilogram. Microplastics were observed in various forms, including fibers, films, foam, and fragments, in both water and soil sediment samples. The majority of microplastics were between 0.1 and 1 mm in size, with blue being the most common color observed in river water and transparent in sediment samples. The ATR-FTIR spectrum analysis indicated the presence of four distinct polymers: polyethylene (PE), polystyrene (PS), polycarbonate (PC), and polyethylene terephthalate (PET). This study provides valuable information on the abundance, distribution, chemical composition, and physical properties of microplastics in the Kemena and Niah rivers.

Microplastics in multimedia environment: A systematic review on its fate, transport, quantification, health risk, and remedial measures

The ever-increasing presence of microplastics in many environmental components has been a cause of worry for humanity due to their small size and potential health risk. Since the last decade, numerous studies have been conducted on the prevalence and dispersion of microplastics. However, at present, there aren't any systematic studies on fate and transport of microplastics that consider multimedia environmental systems and their mitigation measures. Also, there are limited studies on the routes through which humans are exposed to microplastics. In this review, about 380 articles were evaluated to uncover the extent of microplastic fate, transport, and pollution in different environmental components, including soil, freshwater, marine, and atmosphere, as well as its effect on different ecosystems. We gave special attention to understanding many routes and sources of microplastics intended for human consumption and their consequences on human health. Furthermore, we tried to emphasize on the different methods used for sampling, extraction, identification and characterization of microplastics, along with associated benefits and limitations. This study highlighted existing knowledge and gaps in the remediation of microplastics. On this basis, the bottleneck and current challenges have been proposed.

Accumulation and Dispersion of Microplastics near A Submerged Structure: Basic Study Using A Numerical Wave Tank

The presence of microplastics has been reported in most marine environments. Their accumulation can affect the marine ecosystem, and their consumption by small organisms of various sizes can indirectly affect human beings who consume them. Recent observations have reported the pathways and fates of microplastics surrounding man-made coastal structures, such as artificial reefs. However, basic research elucidating the physical behavior near the structure is scarce. We implemented a two-dimensional numerical wave flume simulating intermediate waves with a weak current in a coastal area to investigate the behaviors of microplastics corresponding to parameters such as particle size (0.2, 1, and 5 mm), particle density (900, 1000, and 1100 kg/m3), and submerged artificial structure. The results showed that smaller particles had a stronger horizontal dispersion but a weaker horizontal advection. Installing a submerged structure increased the flow rate above the structure. It also increased particle accumulation upstream and downstream near the edge and corner of the structure. The accumulation was significantly affected by the installation of the structure when the resuspension of microplastics occurred intermittently. This work elucidates the mechanisms underlying the distribution, accumulation, and dispersion of microplastics that are important in predicting the fate of microplastics in the vicinity of artificial structures.

Investigating the evolution of the technologies for collecting microplastics

To stop the dispersion of microplastics in the ecosystem, many technologies for collecting them were designed, tested and developed in the last period. However, a complete and exhaustive comparison of these technologies to guide in the choice and/or in the development of the most suitable appropriate one is missing in the literature. This study investigates the presence of some known technological trends, deriving from the TRIZ (Russian acronym for “Theory of Inventive Problem Solving”) in the behaviour (i.e. the operating principle) of these technologies. To do this, a systematic methodology was followed, which has a general value and consists in analysing the patents relating to these technologies through various bibliometric indexes (i.e. Innovation index, Emergence Score index, Independent Claims index and Technology Cycle Time index). In general, the obtained results did not reveal a clearly identifiable ranking of the behaviour which was unanimously confirmed by all the considered bibliometric indexes. In addition, the average of the scores of the different indexes associated with the different behaviours equalized their differences. However, these results are mainly due to the markedly different evaluations obtained by the Emergence Score index compared to those of the other indexes. From the comparison of the results with the evolutionary trends, it emerged that the operative zone reduction trend was the most confirmed, while the technical system dematerialization was the least confirmed by the bibliometric analysis of all the indexes. In particular, the ranking of the behaviours provided by the Innovation index best confirmed all the evolutionary trends, while that of the Emergence Score index was the worst. In conclusion, this study confirmed the adherence of the development that technologies for collecting microplastics are following to the evolutionary trends through bibliometric analysis: this sequence places magnetic technologies in first place, followed by chemical, fluid dynamics, dynamic mechanics and static mechanics. The analysis of the performances declared in the patents substantially confirms this result.

Microplastic appraisal of soil, water, ditch sediment and airborne dust: The case of agricultural systems

Although microplastic pollution jeopardizes both terrestrial and aquatic ecosystems, the movement of plastic particles through terrestrial environments is still poorly understood. Agricultural soils exposed to different managements are important sites of storage and dispersal of microplastics. This study aimed to identify the abundance, distribution, and type of microplastics present in agricultural soils, water, airborne dust, and ditch sediments. Soil health was also assessed using soil macroinvertebrate abundance and diversity. Sixteen fields were evaluated, 6 of which had been exposed to more than 5 years of compost application, 5 were exposed to at least 5 years of plastic mulch use, and 5 were not exposed to any specific management (controls) within the last 5 years. We also evaluated the spread of microplastics from the farms into nearby water bodies and airborne dust. We found 11 types of microplastics in soil, among which Light Density Polyethylene (LDPE) and Light Density Polyethylene covered with pro-oxidant additives (PAC) were the most abundant. The highest concentrations of plastics were found in soils exposed to plastic mulch management (128.7 ± 320 MPs.g-1 soil and 224.84 ± 488 MPs.g-1 soil, respectively) and the particles measured from 50 to 150 μm. Nine types of microplastics were found in water, with the highest concentrations observed in systems exposed to compost. Farms applying compost had higher LDPE and PAC concentrations in ditch sediments as compared to control and mulch systems; a significant correlation between soil polypropylene (PP) microplastics with ditch sediment microplastics (r2 0.7 p < 0.05) was found. LDPE, PAC, PE (Polyethylene), and PP were the most abundant microplastics in airborne dust. Soil invertebrates were scarce in the systems using plastic mulch. A cocktail of microplastics was found in all assessed matrices.

Assessing Microplastic Prevalence and Dispersion from Saigon Urban Canals via Can Gio Mangrove Reserve to East Sea by Raman Scattering Microscopy

Plastic pollution is one of the significant environmental concerns due to the threefold increase in global plastic waste. Marine microplastics, including petroleum-based plastic pieces and synthetic and artificial fibers smaller than 5 mm, are not only ubiquitous in natural water but also high in wastewater streams due to the direct discharge, transfer and breakdown of plastic items. This research aims to investigate the presence and dispersion of microplastics in the downtown area and coastal suburban area of Ho-Chi-Minh City by using Raman microscopy. As a result, the most common plastics (PE, PET, PA, PP, PVC, PS and PMMA) were detected, and most of them were fibrous shorter than 500 μm. The total microplastics decreased gradually from the urban waterborne (up to 220 MPs/L) via Can Gio UNESCO Mangrove Biosphere Reserve (10 MPs/L) and to the East Sea (3 MPs/L), which reveals the potential role of the mangrove in reducing marine contaminants including microplastics. This study provides important insights into microplastic pollution in the Western Pacific Region, especially the Saigon-Dong Nai river systems, supporting useful data for natural water resources management.

How can “my shoes” affect the amphibian health? A study of the toxicity of microplastics from shoe sole (Polyvinyl chloride acetate) on Physalaemus cuvieri tadpoles (Anura, Leptodactylidae)

More recently, many studies have reported the impacts of microplastics (MPs) on the health of organisms. However, our knowledge of its effects on some vertebrate groups, such as amphibians remains incipient. Thus, we evaluated whether the exposure of Physalaemus cuvieri tadpoles to MPs from shoe soles can induce harmful effects on their health. After 30 days of exposure to different MP concentrations (2 ×105 MP particles/m3 and 4 ×106 MP particles/m3), we noticed that the tadpoles ingested particles with a mean diameter of 148.8 µm and irregular shapes, which accumulated in the intestinal tube. Such accumulation was associated with a lower-body condition index, non-uniformity in the keratinized structures of the jaw sheath, alterations in the intestine position in the abdominal cavity, bowel tube winding condition, and the emergence of dark regions in the intestine like pseudomelanosis. The reduction of superoxide dismutase and catalase activities suggests that MPs have affected the antioxidant system. The minor nitrite production provides evidence that the production of nitric oxide by cells of the intestinal immune system has been affected. In addition, we observed a significant reduction in the size (area, perimeter, and diameter) of MPs after ingestion, which suggests that tadpoles can act as vectors for their dispersal of MPs from shoe soles in the environment. The principal component analysis and integrated biomarker response assessment also confirm that higher MPs concentration induces a more stressful response. Therefore, our study demonstrates that “my sneakers,” while seemingly harmless, can pose a health risk to amphibians.

Fate of river-derived microplastics from the South China Sea: Sources to surrounding seas, shores, and abysses

Microplastics (MPs) in the ocean have been widely recognized as causing global marine environmental problems. To gain a quantitative and comprehensive understanding of oceanic MP contamination, detailed numerical Lagrangian particle tracking experiments were conducted to evaluate the regional oceanic transport and dispersal of MPs in the South China Sea (SCS) derived from three major rivers, Pearl (China), Mekong (Vietnam), and Pasig (the Philippines), which are known to discharge large amounts of plastic waste into the SCS. As previous field surveys have suggested, MP contamination spreads from the surface to the deeper ocean in the water column, we thus considered three types of MPs: (1) positively buoyant (light) MPs, (2) positively buoyant (light) MPs with random walk diffusion, and (3) full 3-D tracking of non-buoyant MPs that are passively transported by ambient currents. Transport patterns of these MPs from the three rivers clearly showed the intra-annual variability associated with seasonally varying circulations driven by the Asian monsoons in the SCS. Many MPs floating during the prevailing southwest monsoon are transported to the northwest Pacific Ocean and the East China Sea through the Luzon Strait and the Taiwan Strait to form MP hotspots. Non-buoyant MPs are broadly transported from the surface layer to depths of approximately 100 m or deeper, where in situ observations are rare. In addition, the buoyant MPs drifting on the continental shelf originating from southern China tend to be pushed toward the shore and beached by northward wind-induced currents more pronouncedly than the non-buoyant MPs. Therefore, the river-derived MPs to the SCS were found to serve as sources to adjacent basins and oceans, to be distributed not only in the upper layer but also in the abyssal ocean (non-buoyant MPs), and to be transported to the shores (buoyant MPs).

Oil spills enhanced dispersion and transport of microplastics in sea water and sand at coastal beachheads

The coastal zone is being under the threat by accumulation of microplastics (MPs), with much of MPs ending up on the beachhead. Oil spills, which frequently happen in coastal zones due to oil pipe leakage or oil drilling, may affect the behavior of MPs in the beachheads. Herein, sea water and sea sand were collected from three different coastal beachheads including Bohai Sea (BS), East Sea (ES), and South Sea (SS), China, to investigate how the oil spills affect the dispersion and transport of MPs in sea water and sand. The oil spills greatly enhanced the dispersion of MPs in all three sea waters by forming MPs-oil-dispersant agglomerates, which increased the electrostatic repulsion and steric hindrance between MPs particles. Accordingly, the aggregation rates of MPs were reduced from 1.7–8.86 nm min−1 to 0.39–1.29 nm min−1. The lowest salinity and highest dissolved organic carbon content in SS sea water favored the highest dispersion of MPs, compared to BS and ES sea water. The improved dispersion of MPs with oil spills enhanced their transport in sea sand with an increase of effluent rates from 0–18.8 % to 5.78–42.2 % for BS and from 30.5–45.2 % to 35.0–60.0 % for SS one. However, the transport of MPs in ES sea sand was lower than 3.62 %, even with oil spills, which was attributed to the strong adsorption of MPs by the rich Fe/Al oxides in ES sea sand through electric attraction. Modeling also showed that oil spills increased the migration rate of 10 mg g−1 MPs accumulated in the surface 0–1 cm sea sand from 6.50–13.8 cm year−1 to 8.17–16.7 cm year−1 after 1500 mm rainfall for 3 years, and the strongest transport of MPs was observed in SS sea sand, with the highest cumulative flux and the longest maximum migration depth as 0.089–0.120 mg/cm2 and 50 cm, respectively. These results indicated that the dispersion and transport of MPs can be enhanced by oil spills, but regulated by sea water salinity for MPs dispersion and sea sand Fe/Al oxides for MPs transport, which advanced our understanding of the transport and transformation of MPs in coastal zones.

Toxicity induced via ingestion of naturally-aged polystyrene microplastics by a small-sized terrestrial bird and its potential role as vectors for the dispersion of these pollutants

In recent years, there has been a growing number of studies on the impact of microplastics (MPs) on biota. However, its effects on birds’ health are poorly understood. Thus, we aimed to evaluate the possible effects of ingestion of naturally-aged MPs by Coturnix Coturnix japonica (11 and 22 MP particles/day/bird, once a day, for 9 days), from different toxicity biomarkers. At the end of the experiment, it was found that the ingested MPs in birds showed a significant reduction in body biomass. Also, an increase in malondialdehyde production in the liver, brain, intestine, and gizzard of the birds, as well as a suppressive effect on hepatic nitric oxide production and superoxide dismutase activity in the liver and intestine were observed. Cerebral catalase activity was reduced in birds exposed to MPs and the cholinesterasic effect (marked by increased acetylcholinesterase activity) was observed in the muscle and brain of these animals. Despite these differences, through the main component analysis, hierarchical clustering analysis, and integrated biomarker response assessment, we observed similar toxicological effects in birds exposed to different amounts of MPs. In addition, the size of MPs was reduced, and their shape was altered as they transited through the gastrointestinal system, which probably explains their accumulation in the liver of birds. An expressive number of MPs are released through the feces of the birds throughout the experiment. As far as we know, this is the first report that associates MPs ingestion by small-sized terrestrial birds with biochemical alterations viz., predictive of oxidative stress, redox imbalance, and cholinesterasic effect, in addition to shedding light on the potential role of these birds as vectors for dispersal of MPs in natural environments

Novel methodology for identification and quantification of microplastics in biological samples

Currently, the evidence of the ingestion of microplastics (MPs) by organisms or the accumulation in different environmental compartments has been achieved using several methodological procedures. However, its uses have not been standardized across studies. In this study, we aim to assess and validate a protocol that can be useful for optimizing the identification and quantification procedures of polyethylene microplastics (PE MPs) in biological samples. Initially, considering that numerous studies filter samples previously digested in cellulosic membranes for isolation and analysis of MPs, we evaluated whether washing these membranes with different reagents could contribute to the complete detachment of particles, as well as to their dispersion in the obtained solutions. However, none of the tested reagents (dimethyl sulfoxide, acetone, ethyl alcohol and chloroform), including purified water, was able to completely remove the MPs adhered to the membranes or facilitate their dispersion in the solutions. On the other hand, we observed that the digestion of the membranes by acetonitrile constituted a procedure that prevents the loss of particles due to adherence, in addition to promoting good dispersion of MPs. Subsequently, we evaluated the use of Neubauer chambers for the quantification of MPs, having observed a good recovery rate (>92%) and results with insignificant variation, in PE MPs solutions with different concentrations (0.15; 0.075 and 0.0375 mg/mL). Ultimately, the validation of the proposed procedures took place from the evaluation of the accumulation of PE MPs in Astyanax spp. juveniles, having demonstrated the efficiency and sensitivity of the method proposed for this purpose. Subsequently, our study provides a methodological alternative that can optimize MPs quantifications in biological samples and reduce the generation of biased or unreliable results.