Seed Dispersal Research Articles

Interspecific competition reduces seed dispersal in an annual plant and slows simulated range expansions

Understanding the factors influencing how fast populations can spread across the landscape will be crucial as species ranges shift due to climate change. While the role of abiotic factors in determining expansion speed has been well studied in theory and empirical research, how competition impacts speed has received far less attention. Here, we investigated how seed dispersal distances change in response to competition and how these changes to distributions of dispersed seeds impact expansion speed. We dispersed four genotypes of the annual plant Arabidopsis thaliana with variation in life history traits into greenhouse mesocosms of either empty habitat or habitat containing the annual grass competitor, Lolium multiflorum. We found that competition decreased both mean and maximum dispersal distance. We then built a simulation model of range expansion with experimental data from this and a prior experiment to explore whether competition slows species expansions primarily through decreasing dispersal or fecundity. We found that competition primarily slows expansion speed through decreases in dispersal, but that when competition impacts both dispersal and fecundity, expansions slow more than with dispersal alone. The genotype with traits associated with longer distance dispersal was the most affected by competition in both experimental dispersal and simulations. This research suggests that not only does competition slow range expansions through decreases in both fecundity and dispersal, but that there may be consequences for evolutionary processes at the leading edge.

Restricted Dispersal and Phenotypic Response to Water Depth in a Foundation Seagrass.

Species conservation and management benefit from precise understanding of natural patterns of dispersal and genetic variation. Using recent advances in indirect genetic methods applied to both adult plants and dispersed seeds, we find that the mean seed dispersal in a threatened marine foundation plant (the eelgrass Zostera marina) is approximately 100-200 m. This distance is surprisingly more similar to that of wind-dispersed terrestrial seeds (~10s to 100s of meters) than the passive dispersal of marine propagules via currents (~10s to 100s of kilometres). Because nearshore marine plants like Zostera are commonly distributed across strong selective gradients driven by bathymetry (depth) even within these restricted spatial scales, seeds are capable of dispersing to novel water depths and experiencing profound shifts in light availability, temperature and wave exposure. We documented strong phenotypic variation and genome-wide differentiation among plants separated by approximately the spatial scale of mean realised dispersal. This result suggests genetic isolation by environment in response to depth-related environmental gradients as one plausible explanation for this pattern. The ratio of effective to census size (or Ne/Nc) approximated 0.1%, indicating that a fraction of existing plants provides the genetic variation to allow adaptation to environmental change. Our results suggest that successful conservation of seagrass meadows that can adapt to microspatial and temporal variation in environmental conditions will be low without direct and persistent intervention using large numbers of individuals or a targeted selection of genotypes.

Extinction and Ecosystem Function Debt Across Dispersal Rate and Behaviour in a Heterogeneous Metacommunity Model

ABSTRACTAimHabitat destruction causes “extinction debt” and is also thought to produce ecosystem function debt, but theory of their magnitude and nature is limited. Heterogeneous landscapes are fundamental to the maintenance of species richness and ecosystem function, while directed or undirected dispersal behaviour, such as dispersal of seeds by animals or by the wind, is also important, especially after habitat destruction. We therefore consider extinction and ecosystem function debt under different dispersal rates and behaviours in heterogeneous landscapes.MethodsWe use a classic heterogeneous metacommunity model to capture the dynamics of competing species in local patches linked by dispersal and varying in environmental conditions. We remove one patch at a time and measure extinction debt and ecosystem function debt by the number/proportion of delayed extinctions and the amount of biomass change, respectively.ResultsWe reveal three species extinction regimes as dispersal increases: (1). species most adapted to the removed habitat are most at risk; (2). similarly adapted species are also at risk; (3). patch removal shifts competitive balance among the few species coexisting at high dispersal, where competition is strong. We find surprisingly that destruction of habitat can hasten the extinction of those species best adapted to harsh environments and that the proportion of diversity at risk from extinction actually increases with dispersal because competition is intense there. Finally, there can be a small ecosystem credit but extinction debt when dispersers reroute to potentially more favourable remaining habitats (directed dispersal), especially when harsh environments are removed. However, ecosystem debt occurs and can be large under undirected dispersal.Main ConclusionsThe magnitude and nature of extinction and ecosystem function debts depend on species dispersal rates and behaviours, as well as the environmental conditions of the disturbed habitats. Conservation actions will be more successful if they consider these factors.

Dandelion-shaped strontium-gallium microparticles for the hierarchical stimulation and comprehensive regulation of wound healing.

The management of full-thickness skin injuries continues to pose significant challenges. Currently, there is a dearth of comprehensive dressings capable of integrating all stages of wound healing to spatiotemporally regulate biological processes following full-thickness skin injuries. In this study, we report the synthesis of a dandelion-shaped mesoporous strontium-gallium microparticle (GE@SrTPP) achieved through dopamine-mediated strontium ion biomineralization and self-assembly, followed by functionalization with gallium metal polyphenol networks. As a multifunctional wound dressing, GE@SrTPP can release bioactive ions in a spatiotemporal manner akin to dandelion seeds. During the early stages of wound healing, GE@SrTPP demonstrates rapid and effective hemostatic performance while also exhibiting antibacterial properties. In the inflammatory phase, GE@SrTPP promotes M2 polarization of macrophages, suppresses the expression of pro-inflammatory factors, and decreases oxidative stress in wounds. Subsequently, during the stages of proliferation and tissue remodeling, GE@SrTPP facilitates angiogenesis through the activation of the Hypoxia-inducible factor-1α/vascular endothelial growth factor (HIF-1α/VEGF) pathway. Analogous to the dispersion and rooting of dandelion seeds, the root-like new blood vessels supply essential nutrients for wound healing. Ultimately, in a rat chronic wound model, GE@SrTPP achieved successful full-thickness wound repair. In summary, these dandelion-shaped GE@SrTPP microparticles demonstrate comprehensive regulatory effects in managing full-thickness wounds, making them highly promising materials for clinical applications.

Earliest evidence of granivory from China (Shanxi Formation) points to seeds as a food source and nursing habitat for insects in the earliest Permian humid tropical forests of Cathaysia.

Three types of plant-insect interactions are identified on seeds from the lower Permian (Asselian) Shanxi and lower Shihhotse formations of the Taiyuan district, North China. This enhances the relatively meagre fossil record of seed predation in global late Paleozoic floras, adding the earliest record of granivory from Cathaysia. The dispersed seeds cannot be attributed with confidence to any particular plant group, but associated fossil leaves belong to a broad spectrum of plants, including Medullosales, Cycadales, Noeggerathiales, Gigantopteridales, Cordaitales, and Voltziales. Among 85 analysed seeds, six showed evidence of predation, referable to three damage types: DT074 and two new damage types that will be added to the forthcoming version of the fossil damage guide (DT274, DT430). These damage features indicate novel strategies of seed exploitation in the earliest Permian of China. The causal agents of the seed herbivory are difficult to resolve with certainty, but possible culprits include representatives of Palaeodictyopteroidea, although we cannot exclude other groups, such as Dictyoptera, Odonatoptera, Archaeorthoptera, Hemipteroidea or early holometabolan insects. The presence of damage features, together with a range of probable defensive structures (hairs, spines, apical horns, and thick integuments), suggests that an active arms race involving insects and plant reproductive structures was already well established by the early Permian.

Evidence of a European seed dispersal crisis.

Seed dispersal is crucial for ecosystem persistence, especially in fragmented landscapes, such as those common in Europe. Ongoing defaunation might compromise effective seed dispersal, but the conservation status of pairwise interactions remains unknown. With a literature review, we reconstructed the first European-wide seed dispersal network and evaluated the conservation status of interactions by assessing each interacting partner's IUCN (International Union for Conservation of Nature) conservation status and population trends. We found that a third of the disperser species and interactions face potential extinction and that 30% of the plant species have most of their dispersers threatened or declining. Our study reveals a developing seed dispersal crisis in Europe and highlights large knowledge gaps regarding the dispersers and conservation status of zoochorous plants, urging further scrutiny and action to conserve the seed dispersal service.

Spatio‐temporal variability in seed production of tree species: implications for restoration in the Cerrado–Amazonia transition zone

The use of propagules collected in ecotonal regions for restoration purposes is challenging because of the mix of species from different vegetation types and reproductive phenological variability. We used a database of the Xingu Seed Network in Brazil, which contains data on 139 native tree species for 8 years (2011–2018) in the Cerrado–Amazonia transition zone, to answer three questions: (1) What is the spatio‐temporal variability in the seed dispersal of tree species, and how does it relate to the species' preferential habitat? (2) Is there a relationship between the seed dispersal period and climate variables? (3) Is the frequency of tree species with different dispersal syndromes equally distributed among biomes and seed‐size classes? Independent of their preferred ecosystem (Amazonia or Cerrado), some species showed substantial spatio asynchrony in fruit dispersal, extending the dispersal period at a regional scale. The seed dispersal period was strongly correlated (rs > 0.6) with precipitation and minimum or maximum air temperature in 41% (n = 57) of the studied species. We showed that three‐quarters of the species (n = 105) dispersed seeds in the late dry and early rainy periods, with little variation among dispersal syndromes. Our findings will enhance the effectiveness of restoration initiatives by increasing the accuracy of predictions of the location and time for collecting seeds in the extensive Cerrado–Amazonia transition zone.

A Study on the Prediction of Dandelion Seed Dispersal Based on Gaussian Probability Distribution

A Study on the Prediction of Dandelion Seed Dispersal Based on Gaussian Probability Distribution

Darwin’s Digestion Myth: Historical and Modern Perspectives on Our Understanding of Seed Dispersal by Waterbirds

Darwin’s Digestion Myth: Historical and Modern Perspectives on Our Understanding of Seed Dispersal by Waterbirds

Correlated evolution of dispersal traits and habitat preference in the melicgrasses.

Seed dispersal is a critical process impacting individual plants and their communities. Plants have evolved numerous strategies and structures to disperse their seeds, but the evolutionary drivers of this diversity remain poorly understood in most lineages. We tested the hypothesis that the evolution of wind dispersal traits within the melicgrasses (Poaceae: Meliceae Link ex Endl.) was correlated with occupation of open and disturbed habitats. To evaluate wind dispersal potential, we collected seed dispersal structures (diaspores) from 24 melicgrass species and measured falling velocity and estimated dispersal distances. Species' affinity for open and disturbed habitats were recorded using georeferenced occurrence records and land cover maps. To test whether habitat preference and dispersal traits were correlated, we used phylogenetically informed multilevel models. Melicgrasses display several distinct morphologies associated with wind dispersal, suggesting likely convergence. Open habitat taxa had slower-falling diaspores, consistent with increased wind dispersal potential. However, their shorter stature meant that dispersal distances, at a given wind speed, were not higher than those of their forest-occupying relatives. Species with affinities for disturbed sites had slower-falling diaspores and greater wind dispersal distances, largely explained by lighter diaspores. Our results are consistent with the hypothesized evolutionary relationship between habitat preference and dispersal strategy. However, phylogenetic inertia and other plant functions (e.g., water conservation) likely shaped dispersal trait evolution in melicgrasses. It remains unclear if dispersal trait changes were precipitated by or predated changing habitat preferences. Nevertheless, our study provides promising results and a framework for disentangling dispersal strategy evolution.

Plant traits determine seed retention times in frugivorous birds: Implications for long‐distance seed dispersal

Abstract Seed dispersal by frugivorous animals is a key process for plant populations and communities. When frugivores consume fruits, seeds are temporarily retained inside their guts until seed deposition. Hence, information on retention times is essential to estimate seed‐dispersal distances. Although it is well known that retention times are affected by the body size of frugivores, there is an important knowledge gap on the effects of plant species traits, such as seed size and pulp content. Here, we comprehensively address whether and which plant traits affect seed retention times in frugivorous birds. On the one hand, we conducted a first set of experiments to assess variation in retention times among 31 plant species dispersed by a single bird species, and a second set to assess variation among five plant species dispersed by a guild of five frugivorous passerines. On the other hand, we conducted a literature review retrieving retention times for 231 interactions involving 155 plant species and 55 bird species from nine different avian orders. Seed size had negative effects on retention times at the three levels addressed in this study: the larger the seeds, the shorter the times. The effects of seed size were higher within a single bird species, intermediate for the small assemblage of five passerine species, and lower for all frugivorous birds included in the compilation. Notably, the effects of seed size on retention times were comparable to that of frugivore body size in Passeriformes. We analysed the effects of the pulp content on a single bird species, but these lacked predictive power. Importantly, the effects of seed size were indirect and mediated by the type of seed ejection (digestive processing): birds generally defecate smaller seeds and regurgitate the larger ones. We provide comprehensive evidence that retention times are intrinsically linked to plant traits and to frugivores' response to such traits, particularly to seed size and the type of seed ejection, respectively. Thus, our study unveils a source of interspecific variability in the capacity of plants to disperse at long distances. Moreover, we provide methodological improvements to trait‐based models that estimate mean retention times and seed‐dispersal distances. Read the free Plain Language Summary for this article on the Journal blog.

A comprehensive comparison of advanced metaheuristic photovoltaic maximum power tracking algorithms during dynamic and static environmental conditions

This study introduces a novel technique for achieving the global peak (GP) in solar photovoltaic (PV) systems under partial shadowing conditions (PSC) using the Dandelion Optimizer Algorithm (DOA), inspired by the dispersal of dandelion seeds in the wind. The proposed approach aims to enhance the power generation efficiency of PV systems across various scenarios, including dynamic uniform, dynamic PSCs, static uniform irradiances, and static PSCs. The proposed approach improves tracking efficiency, provides non-oscillatory steady-state responses, and reduces transients as well as enhancing the dynamic performance of the whole system. Simulation and hardware-in-loop (HIL) experiments demonstrate that the DOA outperforms several state-of-the-art techniques, such as hybrid grey wolf optimizer since-cosine algorithm (HGWOSCA), grasshopper optimization algorithm (GOA), dragonfly optimizer (DFO), particle swarm optimizer with gravitational search (PSOGS), PSO, cuckoo search algorithm (CSA), perturb &observe (P&O), and incremental conductance (INC), achieving average efficiencies of 99.93 %, 88.84 %, 94.48 %, 87.12 %, 88.05 %, 94.79 %, 93.82 %, 85.25 %, and 77.93 %, respectively. These results underscore the DOA's effectiveness in improving maximum power point tracking (MPPT) performance in solar arrays, particularly under challenging dynamic PSC conditions.

How do birds and ants contribute to the recruitment of a tropical tree?

AbstractIn most tropical ecosystems, birds play a crucial role as primary dispersers (Phase 1) by removing seeds from the plant crown. Following primary dispersal, ground‐dwelling animals, notably ants, often disperse seeds secondarily (Phase 2). However, the relative contributions of each phase to seed dispersal effectiveness until plant establishment remains unclear. We combined observational and manipulative experiments to quantify the contributions of birds and ants to seed dispersal effectiveness of the ornithochoric tree Xylopia sericea in the Brazilian Atlantic Rainforest. Birds dispersed 17.2% of diaspores, with a dispersal distance (6.6 m ± 6.7 m) twenty times greater than that of ants (0.3 m ± 0.5 m). Notably, birds often dispersed seeds beyond the parental canopy, where seedling survival is higher. Ants were quantitatively more important to seed dispersal, with relative diaspore removal 221.3% higher than birds. Despite the limited dispersal‐distance by ants, proximity to ant nests increased seedling survival. Phase 1 led to the establishment of 16.26 seedlings per 100 diaspores produced, and Phase 2 increased the number of seedlings established by 28.6% (N = 20.91). Ants enhanced seed recruitment and improved seedling survival both away and near the parental plant, likely due to ant nests providing favorable microsites for establishment. Conversely, plants distant from ant nests performed better when away from the parental plant, underscoring the importance of birds in seed dispersal. Birds promote long‐distance dispersal and ants redistribute diaspores to microsites that increases establishment, so the dispersal of X. sericea is highly dependent on both dispersers.Abstract in Portuguese is available with online material.

Comparative study on mechanical properties and biomineralization of hooks in the diaspores of three epizoochorous plant species

Most of the plants using epizoochory show adaptations to this diaspore dispersal strategy by having the diaspores covered by barbs, hooks, spines or viscid outgrowths, which allow diaspores to easily attach to an animal surface. Many previous studies have been mainly focused on the dispersal distances and efficiency, or effectiveness of diverse attachment structures depending on their size, anatomy, and morphology. However, the knowledge about the mechanical properties of these structures remains rather poor. In this study, we use a combination of scanning electron microscopy, energy dispersive X-ray element analysis and nanoindentation, to examine the microstructure, biomineralization and mechanical properties of single hooks in Arctium minus, Cynoglossum officinale and Galium aparine. Both the biomineralization and mechanical properties of the hooks strongly differ in examined plant species; mechanical properties depend on the biomineralization pattern, such as the accumulation of silicon and calcium. Elastic modulus and hardness decrease in the series C. officinaleG. aparineA. minus. Anisotropic mechanical properties are found between the radial and longitudinal directions in each single hook. By characterizing the mechanical properties and biomineralization of plant hooks, this paper contributes to the understanding of attachment biomechanics related to seed dispersal. Statement of significanceThe dispersal of seeds is essential for plant survival. Many of the plants that use the outside surface of animals to transport the seeds show adaptations to this dispersal strategy by having the seeds covered with hooks. Although these hooks have various sizes, morphologies and anatomical structures, all of them provide mechanical interlocking to animal surfaces. To reduce the risk of interlocking failure, the hooks are usually reinforced by mineralization. However, the relationship between mineralization, mechanical properties and specialized function of plant hooks has been largely overlooked. Here we perform a characterization study on the hooks of three plant species. Our results deepen the current understanding of the mineralization-material-function relationship in specialized hooks of plant seeds.

Phenological gap in fruiting period and dispersal of seeds from alien fleshy-fruited plants by medium-sized carnivores in temperate forests of Central Europe

Some biological differences between native and alien plants are relevant to their dispersal mechanisms. One of them is the fruiting period: it is shifted in time, peaking later than in natives. Here we report the case study showing the temporal distance in fruiting phenology between native and alien plants and their seed dispersal via carnivorous mammals. From 2009 to 2011, scats of badgers Meles meles, foxes Vulpes vulpes, martens Martes spp. (M. martes and M. foina) and possibly also raccoon dogs Nyctereutes procyonoides (N = 820) were collected along transects totaling 30.4 km in length each month from June to November. We analyzed the frequency of occurrence of seeds (FO%) and the seed load in sampled scats; 61.7% of the sampled feces contained seeds of 18 fleshy-fruited native and alien plant taxa, and the most abundant seeds were from species with multi-seeded fruits such as Vaccinium myrtillus (94.6%), Rubus sp. (2.0%), and drupes of Prunus serotina (1.0%). The structure of dominance was characterized by seeds of Vaccinium myrtillus (15.0%), Pyrus sp. (14.8%) and Prunus serotina (13.0%) with aliens reaching high frequency of occurrence (FO%). The shares of seed FO% in the samples differed between alien and native plants. For seed load there were also significant interactions between the status of the seeds (alien or native) and the month of the vegetation period. Our data show the coincidence of two factors – the late fruiting period of alien plants and the decreasing availability of native fruits during the vegetation period. Such a set of factors may promote the dispersal of alien plant seeds by carnivorous mammals, which, unlike migrating birds, are constantly present in autumn. The limited availability of native fruits after their fruiting period, creating a phenological gap, makes alien plants the main source of fleshy-fruits at the end of vegetation period in forest ecosystems; this is expressed in high proportion of alien plants in seed FO%, and in significant interactions in the seed load in carnivore scats.

Unraveling the lncRNA-miRNA-mRNA Regulatory Network Involved in Poplar Coma Development through High-Throughput Sequencing.

Poplar coma, the fluff-like appendages of seeds originating from the differentiated surface cells of the placenta and funicle, aids in the long-distance dispersal of seeds in the spring. However, it also poses hazards to human safety and causes pollution in the surrounding environment. Unraveling the regulatory mechanisms governing the initiation and development of coma is essential for addressing this issue comprehensively. In this study, strand-specific RNA-seq was conducted at three distinct stages of coma development, revealing 1888 lncRNAs and 52,810 mRNAs. The expression profiles of lncRNAs and mRNAs during coma development were analyzed. Subsequently, potential target genes of lncRNAs were predicted through co-localization and co-expression analyses. Integrating various types of sequencing data, lncRNA-miRNA-TF regulatory networks related to the initiation of coma were constructed. Utilizing identified differentially expressed genes encoding kinesin and actin, lncRNA-miRNA-mRNA regulatory networks associated with the construction and arrangement of the coma cytoskeleton were established. Additionally, relying on differentially expressed genes encoding cellulose synthase, sucrose synthase, and expansin, lncRNA-miRNA-mRNA regulatory networks related to coma cell wall synthesis and remodeling were developed. This study not only enhances the comprehension of lncRNA but also provides novel insights into the molecular mechanisms governing the initiation and development of poplar coma.

Biotic and abiotic dispersal of a large-seeded keystone genus in Madagascar

In tropical forests, most plant species rely on frugivorous animals for seed dispersal services. Such mutualisms are imperiled by defaunation, which disproportionately affects large-bodied vertebrates and may impact the interactions of the large-seeded plants they disperse. However, frugivore-mediated seed dispersal (zoochory) may not be the only mechanism ensuring the dispersal of a given plant species. With a focus on large-seeded canopy trees in the genus Canarium in the rainforests of Madagascar, thought to be dispersed by large-bodied lemur species, we investigated the contribution of multiple dispersal mechanisms to the movement of large seeds. Specifically, we (1) examined the potential for dispersal by abiotic factors, such as winds and runoff associated with frequent cyclones, (2) documented the animal species that could effectively contribute to their primary and secondary seed dispersal, and (3) determined how non-lemur and abiotic dispersal compare to the imperiled function of extant, though threatened, lemur primary dispersers. Using field observations and experiments, we found that wind, water, and secondary dispersal can move seeds considerable distances away from the parent plants, though they were less effective at long-distance dispersal than primary dispersers (i.e., large-bodied lemurs). For secondary dispersal, we found that dispersal distance is positively correlated with predation, potentially reducing dispersal effectiveness. Future comparisons with predation of primary or abiotically dispersed seeds will be necessary. Our research highlights the role of understudied mechanisms in the dispersal of large-seeded plants in Madagascar. Understanding the existence and impact of non-lemur dispersers in these imperiled forests can help complete our understanding of the mechanisms that shaped their astounding biodiversity and may mediate their response to ongoing environmental change.

Variation in Seed Morphological Traits Affects the Dispersal Strategies of Chromolaena odorata Following Invasion.

Seed germination and dispersal have an important impact on the establishment and spread of invasive plants. Understanding the extent of intraspecific seed trait variations can enhance our understanding of how invasive plants respond to environmental change after introduction and help predict the dynamic of invasive species under future environmental conditions. However, less attention has been given to the variation in seed traits within species as opposed to among species. We compared seed production, seed morphological traits, dispersal ability, and seedling performance of Chromolaena odorata from 10 introduced populations in Asia and 12 native populations in America in a common garden. The results showed that range (introduced vs. native) and climate affected these traits. Compared with the native population, the introduced populations had higher seed numbers per capitula, lighter seeds, and higher potential dispersal ability seeds (lower terminal velocity) but lower germination rates and seedling lengths. Climatic clines in seed numbers per capitula and pappus length were observed; however, the clines in pappus length differed between the introduced and native populations. Trait covariation patterns were also different between both ranges. In the native populations, there was a trade-off between seed numbers per capitula and seed mass, while this relationship was not found for the introduced populations. These results indicate that C. odorata alters the ecological strategy of seed following invasion, which facilitates its establishment and fast dispersal and contributes to successful invasion in the introduced ranges.

Assessing Nutritional Characteristics and Bioactive Compound Distribution in Seeds, Oil, and Cake from Confectionary Sunflowers Cultivated in Serbia.

Sunflower seeds are key agricultural commodities due to their nutritional and industrial value. This study aimed to analyze the distribution of targeted bioactive compounds and assess the physical properties across 27 sunflower seed genotypes, including parental lines and F1 and F2 hybrids, cultivated in Serbia. Various analytical techniques were employed to determine the chemical composition and physical characteristics of the seeds. This research revealed significant genetic variability in fatty acid profiles, with differences in polyunsaturated and saturated fatty acid levels among the genotypes. Hybrid seeds displayed variations in 1000-seed weight and bulk density compared to parental lines, which exhibited higher essential fatty acid contents and mechanical properties advantageous for industrial processing. These insights highlight the potential for refining breeding strategies to improve seed quality for specific industrial purposes. Overall, this study emphasizes the critical role of genetic selection in enhancing the nutritional and processing qualities of sunflower seeds, offering valuable perspectives for advancing agricultural and breeding practices.

Seed traits and burial state affect plant seed secondary dispersal mediated by rodents

Seed dispersal is an important ecological process and has important implications for plant population expansion and regeneration. Seed dispersal not only reduces the probability of death due to seed density but also facilitates seedling establishment. Many studies have focused on the effect of one or two factors on seed dispersal. However, little is known about studies on the effect of multiple factors and their interactions on seed dispersal. Here, we conducted a field experiment to explore how seed size, soil burial, and seed peeling affect the dispersal and hoarding of seeds of Quercus liaotungensis in dispersal animals. We found that large seeds were preferentially selected by animals, and the predation after dispersal, hoarding after dispersal, predation distance after dispersal, and hoarding distance after dispersal of large seeds were significantly greater than small seeds, which is more beneficial to the plant expansion and regeneration. Soil burial increased the time of seed intact in situ, significantly increased predation in situ, and reduced predation after dispersal, predation distance after dispersal, and hoarding distance after dispersal, which is not beneficial to the plant population expansion and regeneration. Seed peeling reduced the time of seed intact in situ, and the predation after dispersal was significantly greater than that of unpeeled seeds, which is not beneficial to the plant population. We did not find the interactions between seed size, soil burial, and seed peeling on dispersal. The effects of a single factor may be more than their interactions between seed size, soil burial and seed peeling on dispersal. These results implied that seed size, soil burial and seed peeling may affect plant population expansion and regeneration by affecting the dispersal and hoarding of animals.