Disease Prevention Measures Research Articles

Fractional-Order Modeling of COVID-19 Transmission Dynamics: A Study on Vaccine Immunization Failure

COVID-19 is an enveloped virus with a single-stranded RNA genome. The surface of the virus contains spike proteins, which enable the virus to attach to host cells and enter the interior of the cells. After entering the cell, the virus exploits the host cell’s mechanisms for replication and dissemination. Since the end of 2019, COVID-19 has spread rapidly around the world, leading to a large-scale epidemic. In response to the COVID-19 pandemic, the global scientific community quickly launched vaccine research and development. Vaccination is regarded as a crucial strategy for controlling viral transmission and mitigating severe cases. In this paper, we propose a novel mathematical model for COVID-19 infection incorporating vaccine-induced immunization failure. As a cornerstone of infectious disease prevention measures, vaccination stands as the most effective and efficient strategy for curtailing disease transmission. Nevertheless, even with vaccination, the occurrence of vaccine immunization failure is not uncommon. This necessitates a comprehensive understanding and consideration of vaccine effectiveness in epidemiological models and public health strategies. In this paper, the basic regeneration number is calculated by the next generation matrix method, and the local and global asymptotic stability of disease-free equilibrium point and endemic equilibrium point are proven by methods such as the Routh–Hurwitz criterion and Lyapunov functions. Additionally, we conduct fractional-order numerical simulations to verify that order 0.86 provides the best fit with COVID-19 data. This study sheds light on the roles of immunization failure and fractional-order control.

Reception of health data using the Australian Burden of Disease study as an example

Abstract Background Health data, especially on Burden of Disease (BoD), are vital for evidence-based health promotion and effective dissemination is crucial due to resource intensiveness. Germany’s national BoD study is currently transitioning to permanence. Analysing the Australian Institute of Health and Welfare’s (AIHW) well-established collaboration with stakeholders and their reception of health data at national and regional level offers insights for improved knowledge translation. Methods To follow the path of stakeholder engagement, this 3-month project involved scientific exchanges with the AIHW and Department of Health, Western Australia. It compiled communication strategy examples and documents stating examples of BoD data use. A quantitative online survey accompanied by a series of 18 qualitative 45-minute interviews was conducted. The survey implemented via Swift Digital has been running for 20+ days. Interview partners originated from the fields of health research, politics, planning and advocacy for patients or population groups. Results were analysed, illustrated digitally as a booklet and refined in a participatory workshop. Results Communication strategies such as embargoed press and stakeholder releases as well as regular online formats were identified. The survey and interviews displayed the required technical and content-detail level from key facts to raw data and visualisation needs. Noteworthy are the demand to link data to economic aspects like disease expenditure, trends and future scenarios. It elucidated how diverse target groups utilize data to place disease significance and preventive public health measures, aiding dissemination. Alongside, it shows examples of knowledge translation by emphasizing impact over indicators when targeting communities. Conclusions Prioritizing target group identification and employing tailored communication strategies regarding data format and detail level are essential when utilizing new data. Key messages • Analyzing a well-established collaboration with stakeholders offers insights for improved knowledge translation and reveals leveraging engagement strategies for others. • Understanding stakeholder needs is essential for promoting broader health data use in policy and practice, fostering evidence-based decision-making, and enhancing public health outcomes.

Modelling the potential impact of a tax on fruit juice in South Africa: implications for the primary prevention of type 2 diabetes and health financing

BackgroundSouth Africa is experiencing a persistent growth in non-communicable diseases. Diabetes is among the top ten causes of mortality, especially among women, which is partly driven by high levels of added sugar consumption and obesity. To reduce obesity rates and the incidence of diabetes, South Africa introduced a tax on sugar sweetened beverages (also known as the Health Promotion Levy (HPL)) in 2018. The tax is applicable to sugar-sweetened beverages but excludes 100% fruit juice. The government is currently considering extending the tax to include fruit juices. This study models the potential health and economic impact of taxing fruit juices at 20% of the retail price of one liter.MethodsTo analyze the distributional impact of the tax, this study uses extended cost-effectiveness analysis methodology. Data on price elasticities, healthcare cost, income, fruit juice consumption were sourced from the literature and representative national surveys. The potential impact of the tax on diabetes incidence, prevalence, mortality, and financial benefits were estimated for each income group (lowest, quintile 1 to highest, quintile 5).FindingsWe estimate that a 20% tax on fruit juice would avert 156,640 incident cases of type 2 diabetes mellitus over 20 years, with most disease averted occurring among the first- and fifth-income groups. Averted deaths from diabetes would average 2,000 deaths per quintile (for quintiles 1 to 4) and about 2,800 in quintile 5. The improved health resulting from averted incidence and deaths will reduce overall healthcare expenditure by R7.5 billion over 20 years, of which R2.3 billion will occur in the fifth quintile. The South African government will also save about R300 million in subsidizing diabetes-related healthcare cost as a result of prevention; and would raise R8.6 billion in tax revenues per annum. Out-of-pocket expenditure savings will be R303 million and a financial risk protection (money-metric value of insurance) of R4.6 billion over the 20-year period.ConclusionWe conclude that an HPL that significantly raises the retail price of fruit juices would reduce consumption and diabetes-related morbidity and mortality. The tax will also provide significant financial benefits in the form of reduced healthcare costs for both government and households as well as providing financial risk protection to individuals. Health taxes are win-win policies that improve population health and generate revenue for governments to fund public health services delivery and thus improve overall health financing activities of the government. Therefore, population level disease prevention measures such as health taxes are important for achieving universal health coverage.

The Perspectives of Community Pharmacists Toward the Name-Based Rationing System During the COVID-19 Pandemic in Taiwan: Cross-Sectional Survey Study.

In Taiwan's public health system, community-based pharmacists are key first-line health care providers due to their high accessibility. During the COVID-19 pandemic, the pharmacists played a central role in the distribution of these supplies through the Name-Based Rationing System (NBRS), during an acute shortage of masks and testing kits, that helped reduce the spread of the disease. The NBRS, an innovative government-guided strategy developed after the COVID-19 outbreak, provided equitable and convenient access to masks and COVID-19 test kits. This study aimed to investigate (1) Taiwanese pharmacists' knowledge, attitude, and practices (KAPs) of COVID-19, with the intention to assess their preparedness for public health emergencies and their capabilities to implement COVID-19-related policies effectively; (2) their perspectives toward the NBRS; and (3) the association between individual's KAP and corresponding perspectives toward the NBRS. A cross-sectional, web-based survey was conducted in 2 major cities in Taiwan, from June 18 to September 11, 2022, during the peak of the COVID-19 pandemic. To gauge community pharmacists' KAP, a 66-question instrument was developed using guidelines from the Taiwanese Centers for Disease Control, the International Pharmaceutical Federation, and the Taiwanese Pharmacist Association. The instrument's internal consistency reliability was ascertained using Cronbach α (0.819), and its content validity was verified by field experts. Overall, 343 Taiwanese community pharmacists were recruited in the study. Among them, 88% (303/343) scored high on knowledge domain questions related to SARS-CoV-2; 58% (201/343) and 39% (136/343) held positive and neutral attitudes toward COVID-19-related policies, respectively; and 77% (266/343) practiced infectious disease prevention measures in compliance with official guidelines. The results demonstrated a high level of competency in pharmacists in a public health crisis. It revealed that factors including age, pharmacy characteristics, and the number of customers were associated with their perceptions and willingness to continuously participate in the NBRS. Overall, the community pharmacists showed greater support for the COVID-19-testing NBRS compared with the mask NBRS, because of the more favorable influence on the revenue and workforce of the pharmacies and the well-being of the pharmacists. Responses also highlighted concerns about rapid government policy changes and supply dynamics, underscoring the importance of effective communication and considering supply availability in facilitating a successful NBRS. The strong KAP of the community pharmacists justified the government leveraging their expertise in Taiwan's COVID-19 response. While community pharmacies have proven to be essential distribution centers through the NBRS, improving community connections, communication with the government, and supply management are recommended to strengthen the system. These potential approaches aim to ensure successful NBRS implementation and better preparedness for future public health emergencies. Overall, pharmacists have demonstrated their integral role in achieving equitable outcomes and their dedication to public health efforts during crises.

Quantifying the impact of prevalence-dependent adaptive behavior on COVID-19 transmission: A modeling case study in Maryland

The COVID-19 pandemic highlighted the need for robust epidemic forecasts, projecting health burden over short- and medium-term time horizons. Many COVID-19 forecasting models incorporate information on infection transmission, disease progression, and the effects of interventions, but few combine information on how individuals change their behavior based on altruism, fear, risk perception, or personal economic circumstances. Moreover, early models of COVID-19 produced under- and over-estimates, failing to consider the complexity of human responses to disease threat and prevention measures. In this study, we modeled adaptive behavior during the first year of the COVID-19 pandemic in Maryland, USA. The adapted compartmental model incorporates time-varying transmissibility informed on data of environmental factors (e.g., absolute humidity) and behavioral factors (aggregate mobility and perceived risk). We show that humidity and mobility alone did little to explain transmissibility after the first 100 days. Including adaptive behavior in the form of perceived risk as a function of hospitalizations more effectively explained inferred transmissibility and improved out-of-sample fit, demonstrating the model’s potential in real-time forecasting. These results demonstrate the importance of incorporating endogenous behavior in models, particularly during a pandemic, to produce more accurate projections, which could lead to more impactful and efficient decision making and resource allocation.

Experts consensus on standard items of the cohort construction and quality control of temporomandibular joint diseases (2024)

Temporomandibular joint (TMJ) diseases are common clinical conditions. The number of patients with TMJ diseases is large, and the etiology, epidemiology, disease spectrum, and treatment of the disease remain controversial and unknown. To understand and master the current situation of the occurrence, development and prevention of TMJ diseases, as well as to identify the patterns in etiology, incidence, drug sensitivity, and prognosis is crucial for alleviating patients'suffering.This will facilitate in-depth medical research, effective disease prevention measures, and the formulation of corresponding health policies. Cohort construction and research has an irreplaceable role in precise disease prevention and significant improvement in diagnosis and treatment levels. Large-scale cohort studies are needed to explore the relationship between potential risk factors and outcomes of TMJ diseases, and to observe disease prognoses through long-term follw-ups. The consensus aims to establish a standard conceptual frame work for a cohort study on patients with TMJ disease while providing ideas for cohort data standards to this condition. TMJ disease cohort data consists of both common data standards applicable to all specific disease cohorts as well as disease-specific data standards. Common data were available for each specific disease cohort. By integrating different cohort research resources, standard problems or study variables can be unified. Long-term follow-up can be performed using consistent definitions and criteria across different projects for better core data collection. It is hoped that this consensus will be facilitate the development cohort studies of TMJ diseases.

A qualitative study of knowledge, beliefs and misinformation regarding COVID-19 in selected districts in Zimbabwe

BackgroundLack of appropriate knowledge, incorrect beliefs and misinformation misleads people about the risks they face and how best to protect themselves. A study was conducted to explore the knowledge, beliefs and misinformation regarding COVID-19 in Zimbabwe.MethodsA qualitative study was conducted in September-October 2022 with a purposive sample of religious leaders, women leaders, youth leaders, health workers, village health workers, teachers, traditional healers, transporters, and the general population selected from ten sites across the country. In total there were 128 participants (30 key informants and 98 focus group discussion participants). At each site, 3 key informant interviews and one homogenous focus group discussion were conducted using semi-structured interviews and focus group discussion guides, respectively. The data were recorded on audiotapes, transcribed verbatim, and translated into English. Manual thematic analysis of the data was performed.FindingsThree themes were identified in this study: (1) beliefs about COVID-19, (2) knowledge about COVID-19 (knowledge of origin, definition, transmission, signs and symptoms and recommended preventive measures), and (3) misinformation about COVID-19 (regarding its nature, existence and recommended preventive measures). There was awareness of the origin, transmission, signs and symptoms of COVID-19 among the participants. Participants reported that Zimbabwean communities were conversant with public health measures such as maintaining social distancing, wearing masks, and maintaining hand hygiene. However, misinformation was also observed to have circulated among the communities.ConclusionParticipants demonstrated good knowledge of COVID-19. However, the misinformation circulating in the country calls for the government to establish structures to monitor the legitimacy of information coming through different sources and invest in providing information through trusted sources. In any disease outbreak, the government should engage its citizenry to understand their knowledge, beliefs and any misinformation that might influence adherence to disease preventive measures.

Barriers to the Uptake of Tickborne Disease Prevention Measures: Connecticut, Maryland 2016-2017.

Public health programs promote numerous tickborne disease (TBD) prevention measures. However, measures are not frequently or consistently performed. Describe barriers to consistent use of 4 commonly promoted TBD prevention measures. We conducted an online survey (n=1883) evaluating behaviors regarding TBD prevention measures including conducting tick checks, applying insect repellents, showering/bathing, and applying chemical or natural pesticides to residential yards. Respondents could select reasons for never, rarely, or sometimes performing these measures. Descriptive analysis and logistic regression modeling evaluated associations between the 3 most cited barriers for each measure and select demographic variables. The survey was administered to residents in high Lyme disease incidence counties of Connecticut and Maryland, 2016-2017. For tick checks (n=800), the most cited barriers were forgetting (63%), not spending time in tick habitat (28%), and too much trouble (11%). For applying insect repellents (n=1303), the most cited barriers were forgetting (38%), personal safety concerns (24%), and too much trouble (19%). For showering/bathing 2 hours after outdoor activity in tick habitat (n=1080), the most cited barriers were being unaware of the prevention measure (51%), too much trouble (18%), and forgetting (18%). For applying chemical pesticides to yards (n=1320), the most cited barriers were having environmental (45%), pet safety (31%), and personal safety concerns (28%). Lastly, for applying natural pesticides to yards (n=1357), the most cited barriers were being unaware of natural pesticides (31%), having cost concerns (23%), and not being concerned about ticks on property (16%). Forgetting, too much trouble, unawareness, and safety concerns were primary barriers to using several TBD prevention measures. Education regarding effectiveness, safety, and timing may increase uptake of certain measures. These challenges can be difficult to address, highlighting the need for passive TBD prevention measures, such as a Lyme disease vaccine.

Genome sequencing analysis and validation of infestation-related functional genes of Vibrio parahaemolyticus LG2206 isolated from the hepatopancreas of diseased mud crab (Scylla paramamosain) in South China

Vibrio parahaemolyticus (V. parahaemolyticus) is a major bacterial pathogen found in brackish environments, leading to disease outbreaks and great economic losses in the mud crab industry. This study investigated the molecular mechanism of V. parahaemolyticus infecting mud crabs through genome sequencing analysis, survival experiments, and the expression patterns of related functional genes. A strain of V. parahaemolyticus with high pathogenicity and lethality was isolated from diseased mud crab in South China. The genome sequencing results showed that the genome size of V. parahaemolyticus was a circular chromosome of 3,357,271 bp, with a GC content of 45 %, containing 2985 protein-coding genes, denoted as V. parahaemolyticus LG2206. Genome analysis data revealed that a total of 113 adherence coding genes were obtained, including 120 virulence factor coding genes, 37 type III secretion system (T3SS) coding genes, and 277 sequences of T3SS effectors. Survival experiments showed that the mortality was 20 % within 96 h in the 1 × 104 CFU/mL infection group, 90 % in the 3.2 × 105 CFU/mL treatment group, and 100 % in the 1 × 106 CFU/mL treatment group. The LD50 of V. parahaemolyticus LG2206 was determined as 4.6 × 104 CFU/mL. Six genes of znuA and fliD (flagellin encoding genes), yscE and yscR (T3SS encoding genes), and nfuA and htpX (virulence factor encoding genes) were selected and validated by quantitative real-time PCR analysis after infection with 4.6 × 104 CFU/mL of V. parahaemolyticus LG2206 for 96 h. The expression of the six genes exhibited a significant up-regulation trend at all tested time points. The results indicated that the infestation-related genes screened in the experiment play important roles in the infestation process. This study provides timely and effective information to further analyze the molecular mechanism of V. parahaemolyticus infection and develop comprehensive measures for disease prevention and control.

Computed Tomography of Contemporary Occupational Lung Disease: A Pictorial Review.

Occupational lung disease remains one of the most common work-related illnesses and accounts for most deaths from occupational illness. Occupational lung diseases often have delayed manifestation over decades and nonspecific clinical presentations, making it challenging for clinicians to promptly identify the disease and implement preventive measures. Radiologists play a crucial role in identifying and diagnosing occupational lung diseases, allowing for removal of the exposure and early medical intervention. In this review, we share our clinical and radiologic approach to diagnosing occupational lung disease and its subtypes. A collection of sample cases of occupational lung diseases commonly encountered in the modern era at a large Canadian university hospital is included to facilitate understanding. This review will provide radiologists with valuable insights into recognizing and diagnosing occupational lung diseases.

Political Partisanship, Confucian Collectivism, and Public Attitudes toward the Vaccination Policy in Taiwan.

Conventional wisdom suggests that people with a collectivist tradition tend to comply more with the government's regulatory and even coercive disease-prevention policies. Besides this socio-cultural element, political partisanship is also an important aspect relating to people's willingness to cooperate with the government. This study aims to examine the relationships between these two factors and three dimensions of vaccination policy attitudes: common responsibility to take the vaccine, the government's vaccine mandate, and indignation over anti-vaxxers. Using the data from a nationally representative cross-sectional survey conducted in 2022 in Taiwan, this study applies multiple linear OLS regression to examine the relationships between vaccination policy attitudes and Confucian collectivism and political partisanship. Confucian collectivism and political partisanship aligning with the ruling party are associated with supportive vaccination policy attitudes. For those who do not align with the ruling party, the negative attitudes toward the vaccination policy appear in different dimensions according to the party they lean to. Confucian collectivism is prevalent in Taiwan and is related to public attitudes toward vaccination policy. This association is independent of political partisanship. Public health authorities should consider the socio-cultural context and political atmosphere for the effectiveness of disease-prevention measures.

Exploring the genetic variability of the PRNP gene at codons 127, 142, 146, 154, 211, 222, and 240 in goats farmed in the Lombardy Region, Italy

Scrapie is a transmissible spongiform encephalopathy affecting sheep and goats. The prion protein-encoding gene (PRNP) plays a crucial role in determining susceptibility and resistance to scrapie. At the European level, surveillance of scrapie is essential to prevent the spread of the disease to livestock. According to the Regulation EU 2020/772 polymorphisms K222, D/S146 could function as resistance alleles in the genetic management of disease prevention. In Italy, a breeding plan for scrapie eradication has not been implemented for goats. However, surveillance plans based on the PRNP genotype have been developed as a preventive measure for scrapie. This research aimed to describe the polymorphisms at 7 positions within the PRNP gene in 956 goats of the Alpine, Saanen and mixed populations farmed in the Lombardy Region in Italy. PRNP polymorphisms were detected using single nucleotide polymorphism markers included in the Neogen GGP Goat 70 k chip. The K222 allele occurred in all populations, with frequencies ranging from 2.1 to 12.7%. No animals carried the S/D146 resistance allele. However, it has been demonstrated that polymorphisms in the other positions analysed could influence resistance or susceptibility to scrapie outbreaks in different ways. Ten potentially distinct haplotypes were found, and the most prevalent of the three populations was H2, which differed from the wild type (H1) in terms of mutation (S vs P) at codon 240. This study provided additional information on the genetic variability of the PRNP gene in these populations in the Lombardy region of Italy, contributing to the development of genetic control measures for disease prevention.

Identifying risk clusters for African swine fever in Korea by developing statistical models.

African swine fever (ASF) is a disease with a high mortality rate and high transmissibility. Identifying high-risk clusters and understanding the transmission characteristics of ASF in advance are essential for preventing its spread in a short period of time. This study investigated the spatial and temporal heterogeneity of ASF in the Republic of Korea by analyzing surveillance data on wild boar carcasses. We observed a distinct annual propagation pattern, with the occurrence of ASF-infected carcasses trending southward over time. We developed a rank-based statistical model to evaluate risk by estimating the average weekly number of carcasses per district over time, allowing us to analyze and identify risk clusters of ASF. We conducted an analysis to identify risk clusters for two distinct periods, Late 2022 and Early 2023, utilizing data from ASF-infected carcasses. To address the underestimation of risk and observation error due to incomplete surveillance data, we estimated the number of ASF-infected individuals and accounted for observation error via different surveillance intensities. As a result, in Late 2022, the risk clusters identified by observed and estimated number of ASF-infected carcasses were almost identical, particularly in the northwestern Gyeongbuk region, north Chungbuk region, and southwestern Gangwon region. In Early 2023, we observed a similar pattern with numerous risk clusters identified in the same regions as in Late 2022. This approach enhances our understanding of ASF spatial dynamics. Additionally, it contributes to the epidemiology and study of animal infectious diseases by highlighting areas requiring urgent and focused intervention. By providing crucial data for the targeted allocation of resources for disease management and preventive measures, our findings lay vital groundwork for improving ASF management strategies, ultimately aiding in the containment and control of this devastating disease.

Prioritisation of emerging and epidemic-prone diseases and risk factors in India’s three western states using the One Health Risk and Disease (OHRAD) prioritisation tool

BackgroundThere have been numerous outbreaks of infectious diseases to date, and out of them, six events have been declared as Public Health Emergencies of International Concern by the World Health Organization (WHO). The outbreaks have been unprecedented, and the sudden anxiety associated with these makes the situation more complicated. Prioritisation of diseases has been a common process in public health, and it is important to identify infectious diseases that are important for human health. In addition, a One Health approach requires taking also animal origin and related risk factors for infectious diseases into account.MethodsState-level co-creation workshops were conducted in Gujarat, Rajasthan, and Maharashtra, the three western states of India. One Health Risk and Disease (OHRAD) prioritisation tool was used to prioritise emerging and epidemic-prone diseases and their risk factors.ResultsThe risk factors and diseases were scored using the OHRAD tool, and the ranks were obtained. Avian flu by A(H5N1), A(H7N9), and A(H9N2), swine flu by A(H1N1), A(H1N2) and A(H3N2), dengue, rabies, leptospirosis, bovine tuberculosis, salmonellosis, chikungunya, soil-transmitted helminths, and brucellosis were some of the 10 diseases prioritised in all the three western states of the country. Similarly, the presence of mosquito breeding sites near houses or workplaces, open defecation, poor hand hygiene, poor personal hygiene, reluctance to follow disease prevention measures and improper carcass disposal were prioritised as the risk factors for diseases in all three states.ConclusionThe emerging and epidemic-prone diseases and risk factors that are prioritised reflect state-specific scenarios and the gravity of the situation concerning risk factors for disease outbreaks. This can help in allocating resources and increasing efforts in the prevention and control of these diseases.

Histopathologic changes in different organs during mycobacteriosis in guppy (Poecilia reticulata) and japanese medaka (Oryzias latipes)

Total histological sections of laboratory fish Poecilia reticulata and Oryzias latipes with pronounced multiple organ failure caused by Mycobacterium infection (Mycobacterium sp.) were analyzed. The pathogenesis of the disease in question is expressed in the occurrence, in various tissues, of foci of inflammation, subsequently forming a connective tissue capsule — granuloma. These lesions were observed in almost all organs of the abdominal cavity of the fish. The largest and most common lesions were found in the pancreas, kidneys and liver (from 478.6 microns to 858.4 microns), significantly disrupting organ functions, which is a clinical picture characteristic of mycobacteriosis. The occurrence of granulomas and their average sizes in organs such as the intestine and gills were significantly lower and amounted to 328.3 and 468.9 microns, respectively. Data on the stages of granuloma development in different organs of fish are presented. The initial stage of granuloma development consists of clusters of macrophage-like cells, lymphocytes, granulocytes and a small number of cellular residues. At the final stage of development, it is a focus, including an eosinophilic-stained caseous-necrotic nucleus and a shell consisting of epithelioid macrophages. It is shown that there is a relationship between the size parameters of granulomas, the frequency of their occurrence and the degree of organ damage. The localization of granulomas and the degree of their development in guppies and medaka were similar, indicating the absence of species-specific virulence. It follows from this that any warm-water fish species cultivated under controlled conditions are at risk. This requires monitoring for signs of disease and regular preventive measures.

Coagulase-negative staphylococci from bovine milk: Antibiogram profiles and virulent gene detection

BackgroundCoagulase-negative Staphylococcus species are an emerging cause of intramammary infection, posing a significant economic and public health threat. The aim of this study was to assess the occurrence of coagulase-negative Staphylococcus species in bovine milk and dairy farms in Northwestern Ethiopia and to provide information about their antibiotic susceptibility and virulence gene profiles.MethodsThe cross-sectional study was conducted from February to August 2022. Coagulase-negative Staphylococcus species were isolated from 290 milk samples. Species isolation and identification were performed by plate culturing and biochemical tests and the antimicrobial susceptibility pattern of each isolate was determined by the Kirby-Bauer disc diffusion test. The single-plex PCR was used to detect the presence of virulent genes. The STATA software version 16 was used for data analysis. The prevalence, proportion of antimicrobial resistance and the number of virulent genes detected from coagulase-negative Staphylococcus species were analyzed using descriptive statistics.ResultsCoagulase-negative Staphylococcus species were isolated in 28.6%, (95% CI: 23.5–34.2) of the samples. Of these, the S. epidermidis, S. sciuri, S. warneri, S. haemolyticus, S. simulans, S. chromogens, S. cohnii, and S. captis species were isolated at the rates of 11, 5.2, 3.4, 3.1, 3.1, 1, 1, and 0.7% respectively. All the isolates showed a high percentage (100%) of resistance to Amoxicillin, Ampicillin, and Cefotetan and 37.5% of resistance to Oxacillin. The majority (54.2%) of coagulase-negative isolates also showed multidrug resistance. Coagulase-negative Staphylococcus species carried the icaD, pvl, mecA, hlb, sec, and hla virulent genes at the rates of 26.5%, 22.1%, 21.7%, 9.6%, 9.6% and 8.4% respectively.ConclusionThe present study revealed that the majority of the isolates (54.2%) were found multidrug-resistant and carriage of one or more virulent and enterotoxin genes responsible for intramammary and food poisoning infections. Thus, urgent disease control and prevention measures are warranted to reduce the deleterious impact of coagulase-negative species. To the best of our knowledge, this is the first study in Ethiopia to detect coagulase-negative Staphylococcus species with their associated virulent and food poisoning genes from bovine milk.

Prevalence and Associated Factors of Type 2 Diabetes Mellitus Among Chinese Hakka Individuals Aged 35-65 Years: A Cross-Sectional Study.

The prevalence of diabetes in China is increasing, influenced by economic and genetic factors, with varying rates across regions. The Hakka population in Ganzhou city has unique exposures compared to surrounding districts, while limited research reported the epidemiological characteristics of type 2 diabetes mellitus (T2DM) in this population. This study aims to investigate the prevalence and influencing factors of T2DM among the population, thereby establishing a robust foundation for disease prevention and control measures. In 2017, a multistage random sampling method selected 3028 individuals from Ganzhou City's permanent resident population. Physical examinations, blood tests, and questionnaire surveys were conducted for data collection, with binary logistic regression analysis used to examine factors affecting T2DM prevalence. A total of 2978 valid samples were included in this study. The average age of the surveyed population was 52.83±7.88 years, comprising 966 males and 2012 females. The prevalence rates of T2DM were 11.8% and 12.9% in males and females, respectively, while the standardized prevalence rate was recorded as 9.1%. Logistic regression analysis revealed that age (Odds Ratio[OR]=1.05, 95% Confidence Interval [CI]:1.03-1.06), hypertension (OR=2.22, 95% CI:1.71-2.93), family history of diabetes (OR= 3.54, 95% CI: 2.58-4.85), overweight (OR=1.73, 95% CI: 1.20-2.48), high total cholesterol (OR=1.17, 95% CI:1.09-1.27), elevated low-density lipoprotein cholesterol (OR=1.19, 95% CI:1.00-1.40) and serum insulin (OR=1.05, 95% CI:1.03-1.06) were identified as significant risk factors for T2DM, Conversely, a higher level of high-density lipoprotein cholesterol (OR=0.55, 95% CI:0.36-0.84) was found to be inversely related to T2DM development. The prevalence of T2DM in Ganzhou city has significantly increased. The effective implementation of comprehensive management strategies aimed at addressing hypertension, overweight, dyslipidemia, and abnormal serum insulin level is essential for promoting overall well-being and efficiently controlling the prevalence of T2DM.

육계농장유래 ICT-데이터의 분석을 통해 미생물의 정족수감지 (Quorum Sensing) 현상의 발견을 위한 연구

The current study obtained biometric data yielded by information and communication technology (ICT) equipment installed in the mobile phones of farmers from broiler farms and evaluated quorum sensing (QS) phenomenon (QSP), which can be considered as an emergency rescue request signal for chickens and the starting point of a disease. The results indicated that QSP was identified the most rapidly and sensitively during the analysis of digital data yielded by the ICT-equipped automatic feed feeder, followed by the water consumption data. In particular, in the data on daily feed consumption, QSP was identified to be the point where the consumption rapidly decreased by more than 100% compared with that on the previous day. It is believed that if farm owners responded to the QSP or chicken rescue signals and rapidly implemented disease prevention measures, then it would be the wisest field use of ICT data to minimize loss and damage to farms. In addition, the results of this study revealed the priority order of the introduction of ICT equipment. and the most urgent and important for farm management is daily feed consumption data by applying the scientifically based QS approach, for the first time, to our knowledge, in Korea. The results of this study uncovered the importance of installing ICT equipments.

Prevalence rate of primary osteoporosis in China: a meta-analysis

BackgroundPrimary osteoporosis (POP) is recognized as a “silent disease” and often ignored. This meta-analysis aimed to determine the prevalence of POP in the Chinese population over the past 20 years to raise awareness of the disease’s epidemiology, which is hoped to help prevent and treat the condition better.MethodsEight English and three Chinese language databases were searched systematically from January 2002 to December 2023. Relevant data were analysed using Stata 16.0. Meta-regression and subgroup analyses were performed to explore causes of heterogeneity. A funnel plot was further drawn in combination with Egger and Begg tests to determine publication bias.ResultsA total of 45 studies (241,813 participants) were included. The meta-analysis revealed that the overall prevalence of POP in the Chinese population was 18.2% (95% CI: 14.7–21.7%), showing a positive correlation with age. Specifically, prevalence rates were 23.4% (18.3–28.5%) in women and 11.5% (9.1–13.9%) in men. A notable increase was observed within the span of 20 years (16.9% before 2010 and 20.3% in 2011–2020). Notably, regional variations were observed, with southern China reporting a lower prevalence of 16.4% compared to 20.2% in northern China. Meta-regression suggested that sample size significantly influenced the estimation of point prevalence (P = 0.037).ConclusionsOver the past two decades, there has been an increase in the prevalence of POP within the Chinese population. The growing prevalence of older individuals and women further highlights the urgency for tailored disease prevention and control measures.

Psychological Responses of Chinese Medical Students to the COVID-19 Pandemic: Empirical Research Qualitative.

This study aims to explore the psychological reactions of medical students during the pandemic. A qualitative study. A purposive sampling technique was employed, and a qualitative approach was adopted. Semi-structured questionnaires were utilized, and online interviews were conducted. Forty medical students were selected as participants for the interviews. The interview data were analyzed using Colaizzi's seven-step analysis method. The study identified five themes related to the psychological reactions of medical students during the pandemic. Firstly, COVID-19's influence on medical careers was characterized by increased interest and determination in pursuing medical professions, heightened admiration for frontline workers, reinforced commitment to a medical career due to the pandemic, and recognition of the significance of medical education. Secondly, challenges and concerns in medical career pursuit were identified, including negative sentiments towards medical careers during COVID-19 and hesitations and concerns about entering the medical field amidst the pandemic. Thirdly, the impact on mental well-being encompassed diverse anxieties expressed by participants regarding control, transmission, treatment, and intentional spreading of the virus. Participants experienced an emotional progression from calmness to fear and anxiety, with heightened anxiety when relatives or acquaintances contracted COVID-19. Academic delays also contributed to anxiety among medical students. Fourthly, changes in behaviors and mindset were observed, including altered behaviors and mindset in response to the pandemic, as well as increased attention to personal hygiene and disease prevention measures. Lastly, expectations of medical students from government, public, and parents were explored. Understanding the psychological reactions of medical students during public health emergencies is crucial for their well-being and professional development. The findings have implications for medical education and the development of strategies to enhance the psychological well-being of medical students during similar crises.