Diseases Of Waterfowl Research Articles

Comparative analysis of intestinal flora community composition and diversity of Anas platyrhynchos and Anser fabalis in the Yellow River Delta

Abstract The Yellow River Delta wetland is a habitat for wintering waterfowl, where mallard (Anas platyrhynchos) and tundra bean goose (Anser fabalis) are commonly observed. Although the intestinal flora plays a pivotal role in maintaining host health and promoting nutrient absorption, it remains unclear whether the same overwintering habitat and challenging feeding conditions specifically affect the composition and diversity of intestinal flora in both species. Here, high-throughput sequencing was used to compare the intestinal bacterial communities of mallard and tundra bean goose from the same region. There was no significant difference in alpha diversity and beta diversity between the two species. Firmicutes and Proteobacteria were the two predominant phyla, accounting for more than 50.00% of the total. They enhanced the nutrient absorption and metabolic efficiency during overwintering. Catellicoccus was the most dominant genus in the intestinal flora of tundra bean goose, accounting for 33.91%, whereas Clostridium sensu stricto 1 was the most dominant genus in mallard, accounting for 7.34%. This difference is considered to be related to the feeding habits of these two species. No significant differences in the structure and diversity of intestinal bacterial communities were observed between mallard and tundra bean goose, and the similarity of intestinal flora between them was high. This study deepens our understanding of the intestinal flora of two coexisting species with different dietary preferences in the Yellow River Delta. It also lays the foundation for waterfowl disease prevention in this area, carrying significant scientific and practical implications for biodiversity and ecosystem conservation.

Identification of the genetic characteristics of copy number variations in experimental specific pathogen-free ducks using whole-genome resequencing.

Specific pathogen-free ducks are a valuable laboratory resource for waterfowl disease research and poultry vaccine development. High throughput sequencing allows the systematic identification of structural variants in genomes. Copy number variation (CNV) can explain the variation of important duck genetic traits. Herein, the genome-wide CNVs of the three experimental duck species in China (Jinding ducks (JD), Shaoxing ducks (SX), and Fujian Shanma ducks (SM)) were characterized using resequencing to determine their genetic characteristics and selection signatures. We obtained 4,810 CNV regions (CNVRs) by merging 73,012 CNVs, covering 4.2% of the duck genome. Functional analysis revealed that the shared CNVR-harbored genes were significantly enriched for 31 gene ontology terms and 16 Kyoto Encyclopedia of Genes and Genomes pathways (e.g., olfactory transduction and immune system). Based on the genome-wide fixation index for each CNVR, growth (SPAG17 and PTH1R), disease resistance (CATHL3 and DMBT1), and thermoregulation (TRPC4 and SLIT3) candidate genes were identified in strongly selected signatures specific to JD, SM, and SX, respectively. In conclusion, we investigated the genome-wide distribution of experimental duck CNVs, providing a reference to establish the genetic basis of different phenotypic traits, thus contributing to the management of experimental animal genetic resources.

Sensitivity of cell cultures to goose parvovirus

Virus enteritis of geese is one of the most important diseases of waterfowl. This disease causes high lethality in young goslings — up to 16,3–99,6% and significant economic damage to the industry. The infection is widespread in various regions of Russia. It is known that cell culture is an integral part of the laboratory diagnosis of goose parvovirus enteritis. For virus isolation, studying its biological properties, and its accumulation, cell cultures from the organs and tissues of goose embryos and their embryos are widely used. However, the cell cultures’ derivation from goose embryos depends on the seasonality of oviposition in this species of bird. Information on the cultivation of goose parvovirus in other species of cell culture is very limited. To determine the range of cell cultures suitable for virus replication, regardless of the season, is an urgent scientific problem that demands more extensive research. Cell cultures of heterologous species are successfully used for the cultivation of many viruses. In this regard, we studied the possibility of reproduction of the geese parvovirus in cell cultures of different tissues and species of origin: cattle, pigs, goats, sheep, monkeys, cats, geese, and hybrid cultures. It was found that heterologous cell cultures do not support goose parvovirus replication. Moreover, the pig-goose hybrid culture derived by the authors did not show sensitivity to the virus.

Development and Evaluation of a Monoclonal Antibody-Based Blocking Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Novel Duck Reovirus in Waterfowl Species.

The novel duck reovirus (NDRV) is an emerging pathogen that causes disease in various waterfowl species. Since the outbreak, it has caused huge economic losses to the duck industry in China. A rapid, reliable, and high-throughput method is required for epidemiological investigation and evaluation of vaccine immunogenicity. A good first step would be establishing an enzyme-linked immunosorbent assay (ELISA) that could detect NDRV antibodies in different breeds of ducks and geese from the serum and egg yolk. This study used a recombinant NDRV σB protein and a corresponding horseradish peroxidase (HRP)-labeled monoclonal antibody to develop a blocking ELISA (B-ELISA). The cutoff value of the B-ELISA was 37.01%. A total of 212 serum samples were tested by the B-ELISA, and the virus neutralization test (VNT) was the gold standard test. The sensitivity and specificity of the B-ELISA were 92.17% (106/115) and 97.94% (95/97), respectively. The agreement rates between the B-ELISA and VNT were 94.81% (kappa value, 0.896). The B-ELISA could specifically recognize anti-NDRV sera without cross-reacting with other positive serums for other major diseases in ducks and geese. The inter- and intra-assay coefficients of variation (CVs) of the B-ELISA and VNT assays were acceptable. In conclusion, the novel B-ELISA could be a rapid, simple, safe, and economically attractive alternative to the VNT in assessing duck flocks' immunity status and in epidemiological surveillance in multiple waterfowl species. IMPORTANCE NDRV disease is a new epidemic disease in waterfowl that first appeared in China. Compared with the classical DRV (CDRV), NDRV is associated with more severe symptoms, a higher mortality rate, and a broader host range. NDRV has become the prevalent genotype in China. At present, there are no commercially available diagnostic products for the NDRV disease. VNT, as the gold standard serologic test, is not only time-consuming and laborious, but also has high requirements for facilities and equipment, which is not suitable for clinical application. Conventional ELISA requires specific antispecies conjugates that are not currently available. B-ELISA not only has the advantage of higher analysis specificity, but also can be used to test specific antibodies against different waterfowl species, because no species-specific conjugates are required in such detection. Therefore, it is necessary to establish a B-ELISA for the detection of antibodies against NDRV in waterfowl species.

Evaluating the Life-History Responses of Adult Invasive (Bithynia tentaculata) and Native (Physa gyrina) Snails Exposed to a Cu-Based Pesticide (EarthTec® QZ)

The faucet snail, Bithynia tentaculata, is an invasive snail that facilitates outbreaks of waterfowl disease in the Upper Mississippi River of the United States. In response, there is interest in identifying strategies that mitigate its population and spread. In this study we assessed the effects of a copper (Cu) molluscicide, EarthTec® QZ, at three concentrations (0, 0.1 and 0.6mg/L Cu) on adult B. tentaculata and a coexisting native species, Physa gyrina. We found that in the 0.6mg/L Cu treatment, ~ 68% of B. tentaculata snails remained alive after a 4-day exposure whereas all P. gyrina snails died. In contrast, a majority of both snail species remained alive and active after 4 days in the control and 0.1mg/L Cu treatments. Although B. tentaculata demonstrated higher survivorship, it bioaccumulated more Cu than P. gyrina. Additionally, examination of B. tentaculata individuals revealed that females tended to exhibit higher mortality than males.

Data on the epidemiology and pathology of anatipestifer disease in Hungary (2010-2014).

Anatipestifer disease is a contagious disease caused by Riemerella anatipestifer, affecting primarily ducks, geese and turkeys, and characterised by listlessness, diarrhoea, sneezing, nasal discharge, and nervous signs. Sporadically, it occurs in a wide range of other domesticated and wild birds as well. The incidence and characteristics of the disease seen in the three main host species are summarised based on birds submitted for routine laboratory investigation in Hungary over the period 2010-2014. The infection was diagnosed in a higher percentage in geese (9.9%) and ducks (7.5%). It occurred in 5-day-old to 17-week-old geese and 3- to 6.5-week-old ducks, respectively. The pathological lesions were comparable in these two species: enlarged spleen, serofibrinous pericarditis, perihepatitis, airsacculitis, catarrhal enteritis, subcutaneous oedema and hyperaemia over the cranium, mucopurulent exudate in the nasal cavity and occasionally pneumonia, conjunctivitis, purulent arthritis and caseous salpingitis. In some cases, R. anatipestifer produced only secondary lesions, which complicated other diseases such as circovirus infection, mycotoxicosis, mycoplasmosis, or Derzsy's disease. In turkeys, the disease occurred rarely (0.5%) and at an older age (12 to 19 weeks). The lesions most frequently seen were purulent osteomyelitis of the cranium and seropurulent meningitis. Purulent osteomyelitis in the cranium caused by R. anatipestifer infection had not been reported in turkeys previously. To various extents, other local lesions such as serofibrinous pericarditis, airsacculitis, arthritis, and in one case septicaemia were also observed. The high incidence of the disease in waterfowl underlines the importance of appropriate treatment and prevention that should be based on accurate diagnosis and antimicrobial susceptibility testing, proper biosecurity and vaccination with regard to the serotype(s) present on the farm.

Heads in the sand: public health and ecological risks of lead-based bullets for wildlife shooting in Australia

Lead (Pb) is a toxic element banned from fuel, paint and many other products in most developed countries. Nonetheless, it is still widely used in ammunition, including rifle bullets, and Pb-based bullets are almost universally used in Australia. For decades, poisoning from Pb shot (shotguns) has been recognised as a cause of disease in waterfowl and Pb shot has been subsequently banned for waterfowl hunting in many jurisdictions. However, the risks posed by Pb-based bullets (rifles) have not been similarly recognised in Australia. Pb-based rifle bullets frequently fragment, contaminating the tissue of shot animals. Consuming this Pb-contaminated tissue risks harmful Pb exposure and, thus, the health of wildlife scavengers (carrion eaters) and humans and their companion animals who consume harvested meat (game eaters). In Europe, North America and elsewhere, the environmental and human health risks of Pb-based bullets are widely recognised, and non-toxic alternatives (e.g. copper-based bullets) are increasingly being used. However, Australia has no comparable research despite widespread use of shooting, common scavenging by potentially susceptible wildlife species, and people regularly consuming shot meat. We conclude that Australia has its collective ‘head in the sand’ on this pressing worldwide One Health issue. We present the need for urgent research into this field in Australia.

The non-native faucet snail (Bithynia tentaculata) makes the leap to Lake Superior

Abstract The European-origin faucet snail ( Bithynia tentaculata ) has been present in the lower Laurentian Great Lakes since the late 1800s but only very recently reached Lake Superior. Surveys from 2011 through 2013 found faucet snails abundant and wide-spread in the St. Louis River Estuary (reinforcing the estuary's status as non-native species introduction hotspot), with scattered finds elsewhere along Lake Superior's southern shore. Faucet snails were found primarily in littoral areas that had been sampled with D-frame nets. We discuss what is known of the distribution and timeline of the faucet snail in Lake Superior, and summarize morphological features helpful in distinguishing the faucet snail from other gastropods (e.g., the operculum). Given its potential for spread and impacts (including as waterfowl disease vector), the faucet snail warrants an elevated profile in non-native species monitoring, education, and decontamination measures in the upper Great Lakes.

Modeling and analysis of a temperature-driven outbreak of waterfowl disease in the Upper Mississippi River

Bithynia tentaculata is an invasive snail that was discovered in the Upper Mississippi River (UMR) in 2002. In addition to being a threat to native benthos, the snail also harbors parasite associated with annual outbreaks of waterfowl mortality in the UMR. Trophic transmission of parasites between snails and birds occurs during seasonal waterfowl migrations, which can depend intimately on temperature. We developed an annual model for waterfowl disease in the UMR where transmission depends on water temperatures gleaned from empirical studies. By running simulations from annual temperature profiles selected randomly from a normal distribution, we quantified the association between the number of infected hosts and annual average temperatures. Model output demonstrated that as annual average temperatures rise, infected host populations initially increase and then decay after temperatures exceed a certain threshold. Results from this work suggest that increasing temperatures in the region may have a negative effect on parasites, decreasing their transmission and reducing infected host populations.

Infection Patterns in Invasive and Native Snail Hosts Exposed to a Parasite Associated with Waterfowl Mortality in the Upper Mississippi River, USA

Bithynia tentaculata is an aquatic invasive snail first detected in the upper Mississippi River (UMR) in 2002. The snail harbors a number of parasitic trematode species, including Sphaeridiotrema pseudoglobulus, that have been implicated in waterfowl mortality in the region. We assessed the capacity of S. pseudoglobulus cercariae to infect B. tentaculata and native snails found in the UMR. Four snail species (one invasive and three native) were individually exposed to S. pseudoglobulus larvae and all were successfully infected. A subsequent experiment examining infection patterns in invasive and native hosts exposed singly or in mixed treatments revealed no difference in parasite establishment among snail species. Our results add to our understanding of S. pseudoglobulus transmission and provide insight into processes underlying waterfowl disease in the UMR.

Avian Influenza: Virology, Diagnosis and Surveillance

Avian influenza virus (AIV) is the causative agent of a zoonotic disease that affects populations worldwide with often devastating economic and health consequences. Most AIV subtypes cause little or no disease in waterfowl, but outbreaks in poultry can be associated with high mortality. Although transmission of AIV to humans occurs rarely and is strain dependent, the virus has the ability to mutate or reassort into a form that triggers a life-threatening infection. The constant emergence of new influenza strains makes it particularly challenging to predict the behavior, spread, virulence or potential for human-to-human transmission. Because it is difficult to anticipate which viral strain or what location will initiate the next pandemic, it is difficult to prepare for that event. However, rigorous implementation of biosecurity, vaccination and education programs can minimize the threat of AIV. Global surveillance programs help record and identify newly evolving and potentially pandemic strains harbored by the reservoir host.

Differential patterns of infection and life-history expression in native and invasive hosts exposed to a trematode parasite

Non-indigenous species (NIS) are well-recognized as threats to biodiversity worldwide. Yet the interaction between NIS and disease emergence in native habitats remains poorly understood. Bithynia tentaculata is an invasive aquatic snail which is now found in the Upper Mississippi River (UMR). A key concern with this snail is that it harbors trematode parasites (such as Sphaeridiotrema spp.) that have been associated with waterfowl mortality in the region. In this study, we used a combination of field collections and laboratory experiments to better understand the roles that infection competency and host life-history responses play in disease transmission in the UMR. Results from the field and laboratory showed that B. tentaculata infected with Sphaeridiotrema spp. grew to a larger size than uninfected individuals. Although infection was not observed in a number of native species, results from this study suggest that they may still suffer exposure costs (such as reduced growth). Moreover, variability in infection competency between NIS and native snails may dilute or amplify host infection risk. This study reinforces the importance of considering both host life-history responses and competency in systems involving NIS, and provides insight into the factors potentially modulating waterfowl disease in the UMR.

Viruses of waterfowl

Viral disease can cause substantial mortality in wild populations of ducks as well as domesticated geese and ducks. Migrating and captive waterfowl play a role in the dynamics and epidemiology of some viruses that also infect humans, such as influenza virus and West Nile virus. Crowded farm conditions favor the transmission of infectious disease agents among birds. Disease transmission is further facilitated by the comingling of wild anatids with nonmigratory resident waterfowl flocks in zoological parks or on farms. The following article will emphasize the most important viral diseases of waterfowl and briefly cover the newer diseases of suspected viral etiology in this group of birds. As viral detection and identification techniques become more and more sophisticated, and as the study of wildlife diseases increases, new viruses will be discovered and new diseases will be encountered. More research into the viral diseases of waterfowl is needed; the implementation of the latest techniques in molecular epidemiology in addition to the “gold standard” techniques such as virus isolation and histopathology, will yield insight into how viruses move from species to species and from region to region.

Vaccination of chickens against H5N1 avian influenza in the face of an outbreak interrupts virus transmission

Vaccination of chickens with a commercially available killed H5N2 vaccine was being evaluated as an additional tool to enhanced biosecurity measures and intensive surveillance for control of highly pathogenic avian influenza subtype H5N1 disease in Hong Kong in 2002. In December 2002 to January 2003, there were outbreaks of H5N1 disease in waterfowl in two recreational parks, wild water birds, several poultry markets and five chicken farms. In addition to quarantine, depopulation of the affected sheds and increased biosecurity, vaccination of the unaffected sheds and surrounding unvaccinated farms was undertaken on three farms. In at least two farms, infection spread to the recently vaccinated sheds with low rates of H5N1 mortality in sheds when the chickens were between 9 and 18 days post-vaccination. However, after 18 days post-vaccination no more deaths from H5N1 avian influenza occurred and intensive monitoring by virus culture on these farms showed no evidence of asymptomatic shedding of the virus. This provides evidence that H5 vaccine can interrupt virus transmission in a field setting.

Serologischer Nachweis von Antikörpern gegen das Newcastle Disease Virus bei Wassergeflügel mittels Hämagglutinationshemmungs‐Test und Enzym‐Immuno‐Assay

8410 samples from Moscovy duck, Pekin duck and geese were incorporated into examinations of antibodies against the Newcastle Disease virus. A new enzyme-immuno-assay (EIA) for antibody detection in Moscovy duck and Pekin duck was developed using purified antigen from NDV-strain "La Sota". The epidemiology as well as the relation of incidence of the Newcastle Disease in waterfowl was discussed.

Some Infectious Diseases of Waterfowl in the Mississippi Flyway

To establish the presence of selected infectious diseases in a healthy wild waterfowl population, a serologic and parasitologic study was initiated. Sera from 123 Canada geese (primarily Branta canadensis interior) and 179 mallards (Anas platyrhynchos) were screened for antibodies against six arboviruses. There was no neutralizing antibody detected against western viral encephalitis, eastern viral encephalitis, St. Louis viral encephalitis, Venezuelan viral encephalitis, California viral encephalitis, or vesicular stomatitis. Sera from 55 ducks and 12 geese were negative for agglutinating antibodies to Salmonella pullorum, Salmonella typhimurium, and Mycoplasma gallisepticum. Fifty-five duck sera and 11 goose sera were negative for complement-fixing antibodies of ornithosis. The most significant serologic finding was the presence of Newcastle disease antibody. Forty (17 percent) of 236 Canada geese were positive for Newcastle disease antibody. Thirty-seven (14 percent) of 267 mallard ducks had antibody titers for Newcastle disease virus. A limited study of parasitism revealed a low prevalence of Haemoproteus, Leucocytozoon, and microfilariae in both geese and mallards as well as Plasmodium in wild geese. The significance of the serological and parasitological findings is discussed. The occurrence of infectious disease in waterfowl has been well documented (Halloran 1955:332-348, Quortrup et al. 1957, Sciple 1953, Rosen and Bischoff 1950, O'Roke 1931). Most reports of disease in waterfowl have been concerned with spectacular epizootics, individual mortality, an extension of host or geographic range, or a survey of an easily sampled species as part of a domestic animal or human disease problem. While there can be no doubt of the value of these types of reports, they lack continuity and contribute little to understanding the significance of disease in wild populations. This is a report on the initial phase of an infectious disease investigation of selected wa erfowl populations in the Mississippi flyway. Its aim is to establish the presence, prevalence, and significance of infectious disease in this apparently healthy waterfowl population. Previous experience of the birds with disease was determined by the presence of antibodies against a select This study was supported and conducted cooperatively by the University of Wisconsin, Department of Veterinary Science, and the Wisconsin Conservation Department, Division of Research and Planning. Published with the approval of the Director of the Wisconsin Agricultural Experiment Station as Veterinary Science paper N.S. 499. This content downloaded from 157.55.39.148 on Fri, 16 Dec 2016 07:41:45 UTC All use subject to http://about.jstor.org/terms DISEASES OF WATERFOWL * Bradshaw and Trainer 571 number of antigens, or by the identification of protozoan parasites in blood smears. The diseases selected for study were chosen because of a history of waterfowl susceptibility or because of potential opportunity for