The 1064 nm Raman spectroscopy technique was employed to characterize raw, bone fragments (BF), meat fragments (MF), lipid, and residue of meat and bone meal (MBM) samples. Employing three chemometric algorithms, species discrimination models were optimized for the aforementioned sample types, indicating that the presence of BF favored discriminant analysis for poultry samples, yielding a classification error of 0.000. The species discrimination model constructed using Raman spectra combined with PLS-DA algorithm for residue samples was found to be overall optimal, with classification error for four species samples all below 0.061. A detailed analysis of the BF related spectral variables contributing significantly to PLS-DA discriminant analysis was conducted. Finally, this study established the complementarity of Raman spectroscopy in species discrimination analysis from both a physical and chemical components perspective, laying the theoretical groundwork for the development of a high-precision 1064 nm Raman spectroscopic analysis method for MBM species discrimination.
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