Dipstick Method Research Articles

Evaluation of Buffalo Health and Prevalence of Metabolic Disorders in Pulivendula: A Urine Analysis Approach

Aim: The study aims to evaluate the health status of buffaloes in selected villages of Pulivendula, detect potential metabolic disorders, and assess the prevalence of common health issues using urine analysis via the dipstick method. Objectives: The main aim of this study is to identify abnormalities, correlates the findings with nutrition and health data and emphasizes student participation in community-based animal health programs. Materials and Methods: A total of 25 buffaloes from five villages in the Pulivendula region were randomly checked as part of the study. The dipstick method was chosen for urine sample collection and analysis because of its ease of use, affordabilitys and speedy findings. Tests were conducted on parameters including Blood, Bilirubin, Nitrite, Leucocytes, Sp. Gravity Glucose, Protein, pH, Urea, RBC and Ketones. Students also completed surveys that yielded information about the diet, living circumstances, and overall health of the buffaloes. Results and Discussion: The investigation found that a less number of buffaloes had aberrant urine parameters, indicating subclinical illnesses. Proteinuria and ketonuria were more common in buffaloes from settlements with limited access to quality feed and water. The study results revealed a link between dietary behaviours and observed urine abnormalities, highlighting the need for better nutritional management and health monitoring in these locations. This study highlights the importance of the dipstick method in routine veterinary health checks, as well as the need for focused interventions to improve buffalo health and productivity in the Pulivendula region.

Prevalence of urinary abnormalities in asymptomatic school going children by dipstick method.

To determine the prevalence of urinary abnormalities in asymptomatic school children. This observational study was conducted at King Edward Medical University/Mayo Hospital Lahore. Children of 5-15 year of age from Central Model School, Lower Mall, Lahore were included from March to May, 2022. Children having prior renal or systemic disease, on steroids and menstruating girls at the time of urinalysis were excluded. In first screening, children's urine was collected in clean 20mL vessel and examined using urinary dipstick. Children with aberrant results were re-screened after two weeks. Out of total 1600 children, majority were male 1416(88.5%) with mean age 11.92±2.47 years. Urinary abnormalities were observed in 278(17.38%) children on first screening and 86(5.38%) on second screening. Isolated proteinuria was present in 131(8.19%) children on first screening and 26(1.62%) on second screening. Isolated hematuria was present in 100(6.50%) on first screening and 33(2.06%) on second screening. Isolated pyuria was present in 24(1.50%) on first screening and 14(0.88%) on second screening. Combined proteinuria and hematuria were present in 10(0.62%) on first screening and two (0.12%) on second screening. Combined proteinuria and pyuria were present in three (0.19%) on first screening and three (0.19%) on second screening. Combined hematuria and pyuria were present in nine (0.56%) on first screening and five (0.31%) on second screening. Combined proteinuria, hematuria and pyuria was present in one (0.06%) on first screening and three (0.19%) on second screening. Urine screening is necessary for early detection and management of kidney diseases. Marked number of children was found having urinary abnormalities. Asymptomatic proteinuria was the most common finding reflecting underlying kidney pathology going undiagnosed.

Gambaran Hasil Pemeriksaan Keton Urin Segera dan Ditunda Metode Dipstik

Laboratory testing is part of a medical process, both from the beginning to the monitoring of diagnostic development, often the urine tested experiences delays due to late delivery of samples or other factors. One element in the urine is the body of ketone (acetoacetate, 3-hydroxybutyrate, and acetone). Acetone includes the classification of ketones with easily evaporating properties due to temperature. Urine ketone is checked by dipstick methods. There's a 10-90% decrease in urine ketone if urine tests are delayed for 2 hours. Of the 30 urine samples with varying results, which are decreasing and increasing but still touch the normal value limit of urine ketone 0.5 mmol/L. Optimal handling and time of urine sampling for urine ketone testing should be observed to maintain medical protocol and ensure more accurate diagnostic results on the public health service network.

Rapid visual detection of Helicobacter pylori and vacA subtypes by Dual-Target RAA-LFD assay

BackgroundHelicobacter pylori (H. pylori) infects over 50% of the global population and is a significant risk factor for gastric cancer. The pathogenicity of H. pylori is primarily attributed to virulence factors such as vacA. Timely and accurate identification, along with genotyping of H. pylori virulence genes, are essential for effective clinical management and controlling its prevalence. MethodsIn this study, we developed a dual-target RAA-LFD assay for the rapid, visual detection of H. pylori genes (16s rRNA, ureA, vacA m1/m2), using recombinase aided amplification (RAA) combined with lateral flow dipstick (LFD) methods. Both 16s rRNA and ureA were selected as identification genes to ensure reliable detection accuracy. ResultsA RAA-LFD assay was developed to achieve dual-target amplification at a stable 37 °C within 20 min, followed by visualization using the lateral flow dipstick (LFD). The whole process, from amplification to results, took less than 30 min. The 95 % limit of detection (LOD) for 16 s rRNA and ureA, vacA m1, vacA m2 were determined as 3.8 × 10-2 ng/μL, 5.8 × 10-2 ng/μL and 1.4 × 10-2 ng/μL, respectively. No cross-reaction was observed in the detection of common pathogens including Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis, showing the assay’s high specificity. In the evaluation of the clinical performance of the RAA-LFD assay. A total of 44 gastric juice samples were analyzed, immunofluorescence staining (IFS) and quantitative polymerase chain reaction (qPCR) were used as reference methods. The RAA-LFD results for the 16s rRNA and ureA genes showed complete agreement with qPCR findings, accurately identifying H. pylori infection as confirmed by IFS in 10 out of the 44 patients. Furthermore, the assay successfully genotyped vacA m1/m2 among the positive samples, showing complete agreement with qPCR results and achieving a kappa (κ) value of 1.00. ConclusionThe dual-target RAA-LFD assay developed in this study provides a rapid and reliable method for detecting and genotyping H. pylori within 30 min, minimizing dependency on sophisticated laboratory equipment and specialized personnel. Clinical validation confirms its efficacy as a promising tool for effectively control of its prevalence and aiding in the precise treatment of H. pylori-associated diseases.

Kidney Diseases Screening for Asymptomatic School Children by Urine Dipstick Method

Kidney Diseases Screening for Asymptomatic School Children by Urine Dipstick Method

THE EXAMINATION OF URINALYSIS PROFILES OF PREGNANT WOMEN AND TODDLER AS AN EFFORTS OF SCREENING THE POTENTIAL OCCURRENCE OF STUNTING INCIDENTS AND LOW BIRTH WEIGHT BABIES IN PENJARINGAN FLATS, RUNGKUT DISTRICT, SURABAYA

Penjaringan Sari Flats is a solution implemented by the Surabaya City Government to overcome the limited housing in Surabaya. Due to the high number of residents , Penjaringan Sari Flats have a rapid and spread infectious rate. One of the impacts of infectious diseases that may be experienced by pregnant women and also children under five aged is low birth weight (LBW) babies and stunting. One solution to reduce the number of stunting and LBW that can be done is screening at early conditions of infection in pregnant women and toddler using the urine examination method (urinalysis). In examining a urine sample which is examined using the dipstick method (urinalysis using strips), 11 parameters can be read. Three of the eleven dipstick parameters (nitrite, leukocytes, and protein) were stated to be sufficient to screen a patient's initial infection profile. Participants in this community service activity are residents of Penjaringan Sari flats who are female, pregnant, and/or small children aged up to 5 years. The first stage carried out was the introduction of this program and activity plan to the Head of Penjaringan Sari Subdistrict. Data collection on the number of pregnant women and toddlers in Penjaringan Sari Village using observational methods. Data collection was carried out personally by the Head of Community Service accompanied by local women community. The number of pregnant women is 40 and toddlers are 60 as of August 2023. The results of urine sample examinations from 74 patients from residents of Penjaringan Sari Subdistrict are divided into two different categories: pregnant women and toddlers. In the category of pregnant women, around 90% of patients had positive leukocyte results, 60% had proteinuria, and 10% showed positive urine nitrite. In the second group, namely toddlers, the results showed that around 58.13% showed positive leukocytes, around 16.27% experienced proteinuria, and 4.65% of toddler patients had positive nitrite values.

Development of the Visual Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD) Method for Rapid Detection of Vibrio harveyi in Aquatic Animals

Development of the Visual Loop-Mediated Isothermal Amplification Combined with Lateral Flow Dipstick (LAMP-LFD) Method for Rapid Detection of Vibrio harveyi in Aquatic Animals

Establishment of a reverse transcription-recombinase polymerase amplification-lateral flow dipstick method for the dual detection of Israeli acute paralysis virus and chronic bee paralysis virus.

As an important social insect, honey bees play crucial roles in agricultural production, sustainable development of agricultural production, and the balance of the natural environment. However, in recent years, Israeli acute paralysis virus (IAPV) and chronic bee paralysis virus (CBPV), the main pathogens of bee paralysis, have continuously harmed bee colonies and caused certain losses to the beekeeping industry. Some beekeeping farms are located in wild or remote mountainous areas, and samples from these farms cannot be sent to the laboratory for testing in a timely manner, thereby limiting the accurate and rapid diagnosis of the disease. In this study, we used a reverse transcription-recombinase polymerase amplification-lateral flow dipstick (RT-RPA-LFD) method for the dual detection of IAPV and CBPV. RPA primers and LFD detection probes were designed separately for their conserved genes. Primers and probes were screened, and the forward and reverse primer ratios, reaction times, and temperatures were optimized. According to the results of the optimization tests, the optimal reaction temperature for RT-RPA is 37°C, and when combined with LFD, detection with the naked eye requires <20 min. The developed RPA-LFD method specifically targets IAPV and CBPV and has no cross-reactivity with other common bee viruses. In addition, the minimum detection limit of the RT-RPA-LFD method is 101 copies/μL. Based this study, this method is suitable for the detection of clinical samples and can be used for field detection of IAPV and CBPV.

Pathological effects of electronic cigarette exposure in mouse pregnancies

Use of electronic Cigarettes (eCig) has increased worldwide in the last several years. Its use has been reported to be an effective smoking cessation tool and for being healthier than normal cigarette use. Although these reports exist, they lack supportive scientific evidence. Little is known about eCig effects during pregnancy. In the present study, we wanted to determine the effects of eCig exposure at two different important gestational points during mouse pregnancy in order to determine eCig exposure effects during pregnancy. C57/Bl6 mice were exposed to eCig via a nose-only delivery system (Scireq) for 4 days (from 14.5 gestational day (dGA) to 17.5 dGA) or for 6 days (from 12.5 dGA to 17.5 dGA). At the time of necropsy (18.5 dGA) placental and fetal weights were recorded. Maternal blood pressure was determined with a tail occlusion cuff (Kent Scientific) and proteinuria was detrained with the dip stick method. Markers for placental apoptosis were also determined. Treatment with eCig showed: 1) a significant decrease in placental weight and fetal weight (p&lt;0.05) following 4 and 6 days of exposure, 2) higher systolic (p&lt;0.02) and diastolic (p&lt;0.02) blood pressure following 6 days of exposure, 3) increased proteinuria after 6 days of exposure, and 4) differential expression apoptosis markers in the placenta after 4 and 6 days of exposures. We conclude that detrimental effects of eCig coincides with the length of maternal exposure. We confirmed that 4 days of exposure resulted in metrics common to IUGR while 6 days of exposure more closely resembled PE/IUGR pathology. These results could be beneficial in understanding the long-term effects of eCig exposure and the development of placental diseases. This work was supported by the National Institutes of Health grant number 1R15HD108743. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Prevalence of Urinary Tract Infections and Antibiogram of Bacteria Isolated From Children With Sickle Cell Disease in Tanzania.

Introduction Individuals with sickle cell disease (SCD) are particularly vulnerable to urinary tract infections (UTIs) due to immunological deficits and renal abnormalities associated with the disorder. These infections can exacerbate underlying health issues and lead to severe complications if not managed promptly and effectively. Due to the heightened risk and potential consequences of UTIs in this population, this study aimed to determine their prevalence and explore the resistance patterns of causative pathogens among children attending the SCD Clinic at Muhimbili National Hospital (MNH), Dar es Salaam, Tanzania. Focusing on this demographic group, we sought to provide targeted insights to inform better clinical protocols and intervention strategies in regions heavily affected by SCD. Materials and methods This prospective cross-sectional study was conducted at the MNH, Dar es Salaam, Tanzania, with an enrollment over two months from 19th March to 21st May 2015. We diagnosed UTIs in children with SCD using dipstick and culture methods. Antibiotic susceptibility was assessed using the Kirby-Bauer disc diffusion method, evaluating resistance patterns to antibiotics such as ampicillin, cloxacillin, erythromycin, chloramphenicol, ceftriaxone, and trimethoprim-sulfamethoxazole. The diagnostic accuracy of the dipstick and culture methods was validated to ensure reliability in detecting UTIs. Statistical analysis was conducted using Statistical Product and Service Solutions (SPSS) software (Released 2019; IBM Corp., Armonk, New York, United States). Results Among the 250 children, 56 (22.4%) were UTI-positive according to the culture method and 62 (24.8%) were UTI-positive according to the dipstick test. Girls were more likely to be UTI-positive than boys (29.1% and 13.6%, respectively; p-value = 0.011). Escherichia coli was the most common uropathogen, followed by Klebsiella, Staphylococcus, Proteus, and Pseudomonas (44.2%, 26.9%, 21.2%, 3.8%, and 1.9%, respectively). All isolates were resistant to ampiclox. Resistance rates to ampicillin, erythromycin, cotrimoxazole, chloramphenicol, and ceftriaxone were 94.2%, 76.9%, 59.6%, 46.2%, and 21.2%, respectively. Conclusion This study indicated that dipsticks diagnosed more UTIs. The prevalence was higher in girls than in boys. Escherichia coli was the most commonly isolated antibiotic-resistant organism. High resistance levels were observed against the combination of ampicillin and cloxacillin. However,the isolates were less resistant to ceftriaxone. These results call for increased surveillance of resistant uropathogens in the pediatric population with SCD.

Does Proteinuria Measured by Dipstick Method Reflect Reality in Patients with Preeclampsia?

Does Proteinuria Measured by Dipstick Method Reflect Reality in Patients with Preeclampsia?

Effect of Carbohydrate Diet Program on Urine Ketone Positivity with A Long Time on A Diet of One until Three Years

Carbohydrate diet is a diet pattern that reduces carbohydrate consumption. Therefore, the body will use fat as an alternative energy source. The continuous use of fat causes the accumulation of fatty acids so it is often the cause of ketone positivity in the urine. Objective: Find out the effect of a carbohydrate diet program on the positivity of urine ketones. Method this research is an analytic observational study with a cross-sectional approach. The sample used was urine from respondents who had been on a carbohydrate diet for 1 year, 2 years, and 3 years, then urine ketones were examined using the dipstick method. Data analysis was carried out using the Chi-Square test with a confidence value (α = 0.05). Result: The sample in this study amounted to 45 samples, with a total of 23 samples of positive ketones and 22 samples of negative ketones. The results of the Chi-Square test obtained an Asymp.sig value of 0.009 &lt;0.05 (α) which indicates that the carbohydrate diet program has a significant effect on the positivity of urine ketones. The carbohydrate diet program affects urine ketone levels.

Comparison of the efficacy of 75 mg aspirin versus 150 mg aspirin for prevention of preeclampsia in patients at high risk for preeclampsia

BACKGROUND &amp; OBJECTIVE: Preeclampsia is a significant contributor to maternal health challenges. Emphasizing prevention over cure is a guiding principle in healthcare. As a reliable measure to forestall hypertension and its associated complications during pregnancy, aspirin is widely embraced. The early identification of risk factors plays a crucial role in the primary prevention of preeclampsia. Post-risk stratification, aspirin emerges as a pivotal player in the preventive strategy for individuals identified as high-risk for pre-eclampsia. To assess the effectiveness of 75 mg aspirin against 150 mg aspirin in preventing preeclampsia among individuals identified as having a high risk for the condition.&#x0D; METHODOLOGY: A quasi-experimental study was conducted in the Department of Obstetrics and Gynecology, Services Hospital, Lahore, from 27-12-2019 to 27-6-2020.240 patients were enrolled in the study, which was later divided into two groups. Group A received treatment with 75 mg aspirin, while Group B was administered 150 mg aspirin. Efficacy was determined based on whether blood pressure levels were below 140/90 mmHg, and proteinuria was rated as &lt;+1 using the dipstick method.&#x0D; RESULTS: The mean age of the patients was 28.54±6.83 years, and the mean gestational age of the patients was 15.30±1.69 weeks. In the 75 mg group, the efficacy was achieved in 94(78.3%) patients, whereas in the 150 mg group, the efficacy was achieved in 108(90.0%) patients (p-value=0.013).&#x0D; CONCLUSION: This study concludes that the efficacy of 150 mg aspirin significantly surpasses that of 75 mg aspirin in preventing preeclampsia among high-risk pregnant patients.

Human Papillomavirus (HPV16 and HPV18) Infection; Pathogenesis, Vaccination, and Detection by Loop-Mediated Isothermal Amplification with Lateral Flow Dipstick Tests: A Review

Context: Human papillomavirus (HPV) infections contribute to the cause of 15% - 20% of all human cancers. This review aims to examine and provide updated information on various aspects, with a particular focus on topics that are of interest to dermatologists. Evidence Acquisition: Using published studies, the pathogenicity of HPV was investigated. Subsequently, the existing vaccines were explored, followed by a review of the loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) method. Results: For HPV detection, the polymerase chain reaction (PCR), self-sustained sequence replication (3SR), strand displacement amplification (SDA), and nucleic acid sequence-based amplification (NASBA) were used. These methods can have good detection but still have problems. In comparison with nested PCR, the detection of HPV16 and HPV18 using LAMP-LFD has higher sensitivity. Conclusions: Loop-mediated isothermal amplification-lateral flow dipstick is a simple and rapid method for the specific and sensitive detection of HPV. Thus, along with the previous HPV16 and HPV18 diagnostic tools, LAMP-LFD might be useful in field studies or local hospitals.

Rapid and sensitive detection of large yellow croaker iridovirus by real-time RPA and RPA-LFD.

Large yellow croaker (Larimichthys crocea) is a vital marine-cultured species in China. Large yellow croaker iridovirus (LYCIV) can cause a high mortality rate in L. crocea. Rapid and convenient detection of LYCIV is an urgent demand for diagnosis. In this study, rapid and simple recombinase polymerase amplification (RPA), real-time RPA and RPA combined with lateral flow dipstick (RPA-LFD) methods were developed for the detection of LYCIV based on the conserved sequence of the LYCIV major capsid protein (MCP) gene. With these optimized RPA analyses, LYCIV detection could be completed within 20 min at 40°C. Both RPA and real-time RPA could detect viral DNA as low as 102 copies/μL, while the detection limit of RPA-LFD was 101 copies/μL, and there was no cross-reaction with other aquatic pathogens (KHV, CyHV-2, GCRV-JX01, SVCV, LCDV and LMBV). In practical evaluation of RPA, real-time RPA and RPA-LFD methods, the results showed consistency with the general PCR detection. In short, the developed RPA, real-time RPA and RPA-LFD analyses could be simple, rapid, sensitive and reliable methods for field diagnosis of LYCIV infection and have significant potential in the protection of LYCIV infection.

Role of preoperative carbohydrate loading for prevention of perioperative ketoacidosis in elective cesarean delivery.

Starvation of long duration during third trimester of pregnancy is undesirable as it is associated with accelerated fasting leading to hypoglycemia, raised plasma free fatty acid (FFA) levels, and increased plasma/urinary ketones. Carbohydrate (CHO)-rich drinks given preoperatively may ameliorate these deleterious effects. The enhanced recovery after surgery (ERAS) guidelines for perioperative care recommend that oral CHO fluid supplementation 2 h before cesarean delivery may be offered to nondiabetic pregnant women. The aim of the study was to evaluate the role of preoperative oral CHO loading for the prevention of perioperative ketoacidosis in elective cesarean deliveries. One hundred and twenty American Society of Anesthesiologists (ASA) II/III parturients undergoing elective cesarean section under subarachnoid block (SAB) were divided into two groups of 60 each after they gave written informed consent. Group A parturients received 400 ml of filtered water 2 h before surgery. Group B parturients received 400 ml of nonparticulate CHO drink 2 h before surgery. The primary outcome was the incidence of ketonuria studied by the dipstick method. Secondary outcomes included hunger and thirst scores, anxiety score, dominant hand grip strength, and the quality of recovery score. The urine ketone levels were positive (+1) in 8.3% parturients in group A and 1.7% parturients in the CHO group (P value- 0.094). The hunger and thirst scores as well as the modified Beck's anxiety scores were significantly lower in the CHO group (P value- 0.002). Dominant hand grip strength was preserved in both the groups (P value- 0.827). The quality of recovery score was significantly improved in the CHO group (P value- 0.002). No serious adverse effects were noted in either group. Oral CHO drink is safe when administered 2 h before uncomplicated elective cesarean deliveries. It may have a positive influence on a wide range of perioperative markers of clinical outcome.

Improved sensitivity and automation of amulti-step upconversion lateral flow immunoassay using a3D-printed actuation mechanism.

The development of sensitive point-of-care (POC) assay platforms is of interest for reducing the cost and time of diagnostics. Lateral flow assays (LFAs) are the gold standard for POC systems, but their sensitivity as such is inadequate, for example, in the case of cardiac diagnostics. The performance can be improved by incorporating different steps, such as pre-incubation to prolong the interaction time between sample and reporter for immunocomplex formation, and washing steps for background reduction. However, for POC assays, manual steps by the assay conductor are not desired. In this research, upconverting nanoparticles (UCNPs) were coated with poly(acrylic acid) (PAA) and conjugated to anti-cTnI antibodies, yielding non-clustering particles with low non-specific binding. The performance of cTnI-LFA in the PAA-anti-cTnI-UCNPs was compared to thesame UCNPs with a commercial carboxyl surface. A kitchen-timer mechanism was embedded in a 3D-printed housing to produce a low-cost actuator facilitating a timed pre-incubation step for reporter and sample, and a washing step, to enable a multi-step cTnI-LFA with minimized manual labour. PAA-UCNPs showed improved mobility on nitrocellulose compared to those with a commercial surface. The mechanical actuator system was shown to improve sensitivity compared to a labour-intensive multi-step dipstick method, despite pre-incubation occurring during shaking and heating in the dipstick method. The limit of detection decreased from 7.6 to 1.5ng/L cTnI in human plasma. The presented actuator can be easily modified for sensitivity improvement in the LFA for different analytes via pre-incubation and washing steps.

Viscosity-sensitive and AIE-active bimodal fluorescent probe for the selective detection of OCl- and Cu2+: a dual sensing approach via DFT and biological studies using green gram seeds.

A novel dual-mode viscosity-sensitive and AIE-active fluorescent chemosensor based on the naphthalene coupled pyrene (NCP) moiety was designed and synthesized for the selective detection of OCl- and Cu2+. In non-viscous media, NCP exhibited weak fluorescence; however, with an increase in viscosity using various proportions of glycerol, the fluorescence intensity was enhanced to 461 nm with a 6-fold increase in fluorescence quantum yields, which could be utilized for the quantitative determination of viscosity. Interestingly, NCP exhibited novel AIE characteristics in terms of size and growth in H2O-CH3CN mixtures with high water contents and different volume percentage of water, which was investigated using fluorescence, DLS study and SEM analysis. Interestingly, this probe can also be effectively employed as a dual-mode fluorescent probe for light up fluorescent detection of OCl- and Cu2+ at different emission wavelengths of 439 nm and 457 nm via chemodosimetric and chelation pathways, respectively. The fast-sensing ability of NCP towards OCl- was shown by a low detection limit of 0.546 μM and the binding affinity of NCP with Cu2+ was proved by a low detection limit of 3.97 μM and a high binding constant of 1.66 × 103 M-1. The sensing mechanism of NCP towards OCl- and Cu2+ was verified by UV-vis spectroscopy, fluorescence analysis, 1H-NMR analysis, mass spectroscopy, DFT study and Job plot analysis. For practical applications, the binding of NCP with OCl- and Cu2+ was determined using a dipstick method and a cell imaging study in a physiological medium using green gram seeds.

Recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) designed for rapid detection of canine distemper virus.

In the present study, recombinase polymerase amplification (RPA) was combined with the colloidal gold lateral flow dipstick (LFD) method to establish a new, stable, and efficient assay for the detection of canine distemper virus (CDV). We designed a set of specific primers labeled with biotin and a specific probe labeled with dSpacer and C3 spacer, according to the conserved region in the N-terminal gene sequence of CDV. The reaction conditions and systems were then optimized, and the sensitivity and specificity were analyzed for potential clinical application. The results showed that the RPA-LFD assay for CDV detection was successfully established. We also found that the temperature in a closed fist (35°C) is optimal for the RPA reaction. The optimal ratio of primer to probe was 2:1. The minimum detection limit of the RPA-LFD assay was 1 × 101 the median tissue culture infective dose (TCID50)/mL. Using this assay with samples from experimentally infected dogs, CDV was detected in nasal secretions, eye secretions, and blood on the fourth day post infection. In summary, this novel RPA-LFD assay for CDV detection is simple to use, and preliminary findings indicate its high specificity and sensitivity.

Relationship between Human Body Weight and Specific Gravity of Urine

The objective of this study was to compare the relationship between human body weight and specific gravity of urine. Urinalysis test is not a painful procedure and it is used to analyze the abnormalities. The comparison between urine density and water density is done by the help of urine specific gravity. The condition of diabetes insipidus can be reported during concentrated urine causes the thirst and removal of excess amounts of dilute urine. Fresh sample of urine was collected in a container and immediately a dipstick method was applied for a few seconds the stick was dipped to check the color change. Statistical analysis was done by applying the rules of M-Stat by using t-Test for getting results. This project is a questionnaire based study about the specific gravity of urine of patients. Urine samples of the patients were collected and then measured the weight of the patients. Results obtained that there was not any relationship between human body weight and specific gravity of urine. It was concluded that there was no significant relationship observed between human body weight and specific gravity of urine.