Diospyros Celebica Research Articles

GC-MS analysis of three Diospyros species and evaluation of its in vitro rat intestinal a-glucosidase and FRAP activity

Abstract. Hariyanto NA, Ramadhan R, Raharjo Y, Firdaus YFH, Kurnia IT, Lestari DA, Suwandari S, Mahmuda NF, Nathanael J, Phontree K, Phuwapraisirisan P, Riyadi L, Rahman A, Abdulgani N. 2024. GC-MS analysis of three Diospyros species and evaluation of its in vitro rat intestinal a-glucosidase and FRAP activity. Biodiversitas 25: 2442-2453. The vast diversity of natural products has long been recognized as a potentially valuable source of bioactive compounds derived from nature. The Diospyros species, belonging to the Ebenaceae family, thrive in tropical forest regions and have been traditionally used for treating various disorders such as ischemic stroke, hypertension, and infectious diseases. This research aims to assess the phytochemical constituents, rat intestinal a-glucosidase, and Ferric-Reducing Antioxidant Power (FRAP) of non-polar (n-hexane) and semi-polar (dichloromethane) extracts from three Diospyros species, i.e., Diospyros buxifolia (Blume) Hiern; Diospyros celebica Bakh.; Diospyros confertiflora (Hiern) Bakh). Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified numerous bioactive secondary metabolites in the extracts. The study revealed that the semi-polar extracts of D. buxifolia exhibited potent rat intestinal a-glucosidase inhibitory activities, with an IC50 value of 33.31±3.23 ug/mL. The IC50 of acarbose as a positive control was 6.31±0.31 ug/mL. The FRAP test demonstrated that the non-polar and semi-polar leaf extracts of the three Diospyros species from East Kalimantan are potential sources of natural antioxidants, with the IC50 in the range of 6.48±0.38 umol Fe/g - 11.35±0.16 umol Fe/g. The results of this study confirm the therapeutic properties of leaf extracts derived from 3 Diospyros species, which align with their traditional medicinal use.

Phenotypic and Estimated Genetic Variability in Endemic Diospyros celebica Bakh. and Widely-spread D. blancoi A.Dc. Cultivated at Purwodadi Botanic Garden, Indonesia

The endemic Diospyros celebica and widely spread D. blancoi are two valuable species of Diospyros (the genus of persimmons and ebonies) that have been conserved at botanic gardens in Indonesia. Understanding the biological contrasts between endemic and widely spread species is essential for plant conservation. This study aims to compare the phenotypic and estimated genetic variability between the endemic D. celebica and the widely spread D. blancoi to support their conservation. Fifty-three morphological characters from fifteen selected living plant specimens at Purwodadi Botanic Garden were measured directly or using variable encoding methods. Unpaired two-samples Wilcoxon test and hierarchical cluster analysis were used to compare morphological variations between both species. Further, genetic variabilities were estimated from encoded morphological characters using GenAlEx 6.503 software. The two Diospyros’ morphological characters were significantly different, except for the lamina length, pairs of leaf vein, number of flowers in each inflorescence, and the fruit sulci presence. The cluster analysis successfully distinguished D. celebica from D. blancoi according to thirteen comparable morphological characters. The endemic D. celebica has lower genetic variability (female/bisexual %P = 86.21%, He = 0.259, uHe = 0.277; male P = 54.29%, He = 0.195, uHe = 0.234) than the widely spread D. blancoi (female/bisexual %P = 86.76, He = 0.311, uHe = 0.355). Due to the importance of genetic diversity in plant conservation, both species’ population size needs to be maintained, if not increased, to preserve the existing phenotypic and genetic variabilities within the cultivated populations at the botanic garden.

FLAVONOID COMPOUND FROM ETHANOL EXTRACT OF Diospyros celebica LEAVES AS AN ANTIDIABETIC CANDIDATE

Ethnobotanically in Indonesia, Diospyros celebica Bakh is recognized as having antidiabetic activity. This study aimed to isolate antidiabetic compounds from the leaves of Eboni (Diospyros celebica). D. celebica leaves were dried and extracted with 96% ethanol, then fractionated and purified guided by antioxidant activity test, α-glucosidase inhibitory test, and dipeptidyl peptidase-4 (DPP-4) inhibitory test until bioactive isolates were obtained. The isolates were identified and their structures were determined based on NMR 1H, 13C, 2D, and LCMS/MS spectroscopic data. Extracts and fractions have potential antioxidant (DPPH), glucosidase inhibitor, and DPP-4 inhibitor activities as well as containing flavonoids and polyphenols. From the ethyl acetate fraction, isolate 26a-2 namely 3,5-digalloyl-(-)- epiafzelechin was obtained which was active as a DPPH scavenger, glucosidase inhibitor, and DPP4 inhibitor. The extracts, fractions, and flavonoid compounds in D. celebica leaves have the potential for antidiabetic drug candidates.

Perdagangan dan Eksploitasi Kayu Eboni di Sulawesi Tengah pada Masa Kolonial: Sebuah Tinjauan Awal

Diospyros celebica Bakh, commonly known as ebony or black ebony,is one of the endemic species found on the island of Sulawesi, Indonesia. This exquisite wood has been in high demand globally due to its aesthetic appearance, resulting in its trade for many years since the pre-colonial period. This paper aims to explore the history of ebony in the global trade network and its exploitation in Central Sulawesi in the first half of the 20th century. This paper relied on a variety of sources including newspapers, government documents, botanical magazines, and other literature related to the topic. This paper reveals that the exploitation of ebony during the colonial period was massive, especially during the 1930s. Europe, America, and Japan were the primary export destinations for ebony, with Japan being the largest consumer of this wood. The significant exploitation of ebony raised concerns for the Dutch East Indies as it became increasingly scarce by the end of the 1930s.

Pemilihan jenis pohon untuk pengembangan arboretum Kampus IV Universitas Khairun

Arboretum is an artificial forest that collects various types of plants for educational purposes and plant preservation. The development of an arboretum requires basic information on environmental conditions and tree species suitable for planting. Therefore, this study was carried out with the aim of selecting tree species for the development of the arboretum at Campus IV, Khairun University. The research was carried out at Campus IV of Khairun University which included a study of land conditions, a literature review of local tree species, and tree species selection. A potential location for an arboretum is the triangular park at the campus entrance. This location was planted with the Angsana species (Pterocarpus indicus) in 2019, which has now grown to the pole and tree stage with an average height of 6 m and an average crown diameter of 4.1 m. The soil has a clay texture with a pH of 5.2 and humidity of 55%. Thus, the location can be planted with intolerant tree species on the edges and tolerant species in the middle which has been shaded by angsana vegetation. The tree species selected for species enrichment in the arboretum are local species in the Wallacea region, namely Canarium indicum, Palaquium spp., Syzygium malaccense, Vitex cofassus and Diospyros celebica; timber wood-producing species, namely Swietenia macrophylla and Ochroma pyramidale; producer of gaharu NTFPs, namely Aquilaria malaccensis; and shade-tree for aesthetics, namely Tabebuia rosea.

Plant Inventory Constituent of Vegetation Structure of Balikpapan Botanical Garden Area Embung Wain Route East Kalimantan

Plants are important living things that have a role as oxygen providers for other living things. In addition, plants can also be stores of biomass and carbon stocks. The vegetation of the botanical garden area has great potential for maximizing the function of plants and as a place for the conservation of various types of plants. Therefore, it is necessary to conduct continuous inventory (monitoring) to prevent extinction and to update plant data that has never been identified. This research was conducted to identify plants in the Balikpapan Botanical Garden area, especially on the Embung Wain track, which is the first track frequented by visitors for education.Data retrieval using the method roams all regions on the track. The results of the study revealed as many as 117 species of plants, consisting of 59 families. The members of the plant family that are found are Asteraceae (6 species), Euphorbiaceae (6 types), Fabaceae (7 types), Moraceae (7 types), Poaceae (7 types), and Zingiberaceae (6 types). Endemic plants found in this area are Agathis borneensis, Diospyros borneensis, Diospyros celebica, Eusideroxylon zwageri, Etlingera balikpapanensis, and Eurycoma longifolia.

Phytochemical analysis in conjunction with in vitro a-glucosidase inhibitory and antioxidant activities of three Diospyros species from East Kalimantan, Indonesia

Abstract. Ramadhan R, Rosyidah N, Firdaus YFH, Kurnia IT, Lestari DA, Phontree K, Phuwapraisirisan P, Riyadi L, Rahman A, Abdulgani N, Maretna SA, Fadhila KN. 2023. Phytochemical analysis in conjunction with in vitro a-glucosidase inhibitory and antioxidant activities of three Diospyros species from East Kalimantan, Indonesia. Biodiversitas 24: 4783-4790. Locals who live in forested areas have long relied on Non-Timber Forest Products (NTFPs) for their traditional ceremonies, food, and medicine. As non-timber forest products, medicinal plants are of interest since they are used by locals in East Kalimantan regions, Indonesia, for local traditional medicine. In the search for new potential antidiabetics and antioxidants created from natural resources, the authors concentrated on medicinal plants traditionally used in East Kalimantan and ethnopharmacological data. The purpose of this study is to evaluate the dual roles of non-timber forest products, such as three Diospyros L. species, in a-glucosidase inhibition, antioxidant (free radical scavenging activity against DPPH and ABTS), and CUPRAC in-vitro activities. This study showed that the strongest a-glucosidase inhibitory activity was found in ethyl acetate and ethanol residue extracts of Diospyros celebica Bakh., with IC50 values of 4.5804±0.14 ug/mL and 3.8236±0.19 ug/mL respectively, which were more potent than quercetin as a positive standard. Additionally, the antioxidant test revealed that leaf extracts of three Diospyros species from East Kalimantan are excellent sources of natural antioxidants, as demonstrated by the SC50 values. The findings of this study support and validate the use of leaf extracts of three Diospyros species in traditional East Kalimantan medicine.

Characterization of rhizosphere fungi of the ebony plant (Diospyros celebica Bakh.) which has the potential to produce Indole-3-Acetic Acid and Gibberelic Acid Phytohormones

Ebony (Diospyros celebica Bakh.) is a slow-growing species that is an impacting factor in the extinction of this species. Efforts to reduce the ebony population can be performed by employing microbes to stimulate ebony growth. This study aimed to obtain bacterial isolates with the potential of producing IAA and GA3 that can be further utilized as biofertilizer agents. Research stages encompassed soil sample isolation, isolation of bacteria, and analysis of IAA and GA3. The research was in complete randomized design (CRD). Thirteen isolates were successfully purified and analyzed with their IAA and GA3 hormones. The highest IAA concentrations were observed in EP1b at 48 incubation hours (44.39 ppm), EP3d at 72 incubation hours (34.93 ppm), and EP3c at 72 incubation hours (34.48 ppm). EP2c, EP2e, and EP2a produced the highest GA3 concentration at 24 incubation hours which were 3.88 ppm, 3.88 ppm, and 3.81 ppm, respectively.

Isolation and Identification of Phenolic Compounds from Ebony Plant Leaves (Diospyros celebica Bakh.)

Isolation of phenolic compounds from Ebony plant leaves (Diospyros celebica Bakh .) was done by maceration technique with methanol solvent. The concentrated extract of methanol dissolved with distilled water and partition was extracted with ethyl acetate. The concentrated ethyl acetate extract dissolved with methanol solvent and partitioned with n-hexane. Then Thin layer chromatography was analyzed before column chromatography, and the eluent suitable for separation is chloroform: methanol 90:10, 80:20, 70:30,60:40,50:50 (v/v) as mobile phase. The compounds were purified with a thin layer preparative and crystallization yielding yellow paste weighing 7.5 mg with Rf = 0.45. The test purity of compounds is done by thin layer chromatography showing a single spot with eluent chloroform: ethyl acetate (50:50) v/v and n-hexane: ethyl acetate ( 80:20) v/v. The following compound was further indicated by Ultraviolet-Visible Spectrophotometer (UV-Vis), Fourier Transform Infra Red Spectrophotometer (FT-IR), and Proton Nuclear Magnetic Resonancy Spectrophotometer (1H-NMR) was estimated as a phenolic methyl gallate, phenolic acid.

Carbon Stocks Potential and Economic Value Valuation of Carbon Stocks in Ebony Stands

One of the important activities to achieve the implementation of REDD+ activities is the measurement and reporting of carbon stocks (Masripatin et.al. 2010). Information on the potential for carbon stocks in a plant stand or forest area can provide an overview for related parties of the potential carbon stocks and the added value that will be obtained if it maintains and develops a certain plant species to support the reduction of greenhouse gas emissions. This study aims to determine the potential of biomass, carbon stocks, and economic valuation of the carbon stocks potential of several Ebony stands contained in the Kawanua Arboretum BP2LHK Manado. The types of Ebony stands that were the object of this study were Dyospiros celebica, Dyospiros rumphii, and Dyospiros ebenum. Determination of biomass potential and carbon stocks using the allometric equation. The economic valuations of each potential carbon stocks of Ebony stands are obtained based on the selling value of carbon set by the World Bank Group. Based on the results of the study, it is known that the carbon stocks potential of each Ebony stand, namely Diospyros rumphii, is 74.246 tons/ha, followed by the potential carbon storage in the Diospyros celebica stand of 67.768 tons/ha and Diospyros ebenum of 64.977 tons / ha, while the economic valuation value of the highest carbon storage potential is found in the Diospyros rumphii stand of USD 148.492 followed by Diospyros celebica is USD 135.536 and Diospyros ebenum is USD 129.954

Untargeted metabolomics analysis of Diospyros celebica Bakh. from three different geographical origins in Sulawesi island using UHPLC-Q-Orbitrap HRMS

Untargeted metabolomics analysis of Diospyros celebica Bakh. from three different geographical origins in Sulawesi island using UHPLC-Q-Orbitrap HRMS

Metabolite Profiling of Ebony (<i>Diospyros celebica</i> Bakh) Leaves and Wood Extracts Using LC-MS/MS

Ebony (Diospyros celebica Bakh) is an endemic forest tree species found in Sulawesi whose basic information on its metabolite profile is still lacking. The objective of this research was to separate and identify putatively metabolite present in the leaves and wood of ebony across Sulawesi. Separation and identification of ebony metabolites were carried out using UHPLC-Q-Orbitrap HRMS analysis. Using ultrasonication with ethanol as the extracting solvent, we used powdered ebony leaves and wood. The results showed that the metabolites contained in the leaves and wood of ebony were 59 metabolites. About 14 compounds were found in the leaves and wood, 21 compounds in the wood, and 24 compounds in the leaves. The identified metabolites are flavonoids, terpenoids, amino aldehydes, alkaloids, quinones, steroids, amino acids, fatty acids, and saccharides. Clustering of ebony using principal component analysis obtained leaves and wood groups using peak area of known compounds as the variable.

Collecting wood core samples from Macassar ebony (Diospyros celebica Bakh.) for multi-purpose analysis using pickering punch

Sample collection activities for a study of population genetics across the natural distribution of targeted tree species require a lot of resources, mainly if repeated field visits are necessary. Conventionally, population genetic studies use good sample material like leaves. In addition, cambium or small pieces of fresh wood can be used to replace leaf samples. Currently, restrictions from the permit regulation have caused only a limited number of samples that can be collected. Therefore, efficient use of samples must be designed to maximize their uses for research. Due to the small amount of successfully sampled materials, hence there are limitations to extend their uses for other analyses and are often sufficient only for genetic analysis. Therefore, innovation in sampling methods using pickering punch (https://www.agroisolab.com/pickering-punch) to collect ebony wood cores in this study is required to cover multi-analyses not only limited to genetics but also for other analyses such as isotopes, near-infrared spectroscopy (NIRs), anatomy, and chemical compounds.•Pickering punch is recommended for efficient wood core sample collection from ebony standing trees.•323 wood core samples were successfully collected from 16 natural populations across Celebes (Sulawesi).•Multi-analyses studies on sampled wood cores are possible for ebony wood identification (e.g., species and origin/provenance).

Analisis Mikrohabitat Eboni (Diospyros celebica Bakh.) pada Kawasan Hutan Tombolo Resort Balocci Taman Nasional Bantimurung Bulusaraung Provinsi Sulawesi Selatan

The study aimed to analyze the pattern of ebony distribution (Diospyros celebica Bakh) and the relationship of microhabitat factors with variable density and dominance. This research was conducted in the Tombolo Resort Balocci Forest Area of Bantimurung Bulusaraung National Park in South Sulawesi Province. Data collection is carried out in a plot measuring 100 m x 100 m divided into 100 subplots measuring 10m x 10m, covering the individual number of trees and the area of the base field on each research sub-plot. Microhabitat factors include pH, marbles, soil depth, header closure, and the proportion of sub-plot surfaces covered in outcropping stones. The results showed that (1) the pattern of ebony distribution at the location of this study is clustered, (2) the correlation value of pH and density shows a negative and insignificant relationship (p>0.05), the correlation of marbles with density shows a positive and significant value (p<0.05), the closing correlation of the header, the depth of the soil with a positive but insignificant density (p> 0.05), while the correlation of the proportion of sub-plot surfaces covered in outcrops and densities showed a negative but significant relationship (p<0.05), (3) the correlation value of pH with negative and insignificant dominance (p>0.05), the correlation of the proportion of outcrop stones with negative and significant dominance (p<0.05), the correlation of heading closure, marbles with positive and significant dominance (p<00.05), soil depth correlation and positive but non-significant dominance (p> 0.05).

Reproductive phenology period of the five threatened species in Cibodas Botanic Gardens

Agathis borneensis, Castanopsis argentea, Diospyros celebica, Merrillia caloxylon, and Saurauia bracteosa are some threatened species, which have been planted in Cibodas Botanic Gardens as a garden collection. As part of the plant conservation program, their vegetative and reproductive phenology knowledge is crucial to understand when to harvest the seeds used for further propagation and restoration. The study aimed to investigate the phenology phases of these species as well as the impact of microclimates. The study used a descriptive-quantitative analysis based on the records of the exploratory-inventory observation of flowering and fruiting phase data and microclimate circumstances in 2018. Except for precipitation, the data revealed that CBG’s microclimate was considerably less diversified. The results also described that A. borneensis has flowering and fruiting almost throughout the year. C. argentea flowers at the end of the year and then develop into fruit at the beginning of the next subsequent year. D. celebica is also a low-intensity flowering plant that bears fruit almost all year. S. bracteosa has a long period of flowering from May to the end of the year. And then it started to develop into fruits from July to the end of the year and continued to January of the following year. Unfortunately, M. caloxylon is not shown a reproductive stage throughout the year. According to the findings, reproductive phases prominently occur early and at the end of the year, along with the rainy season. It also implied the appropriate time for seed harvesting conducted during these periods.

Tree species diversity on ebony habitat with different degradation levels in Sulawesi

Ebony (Diospyros celebica Bakh.) is endemic species to Sulawesi that is experiencing population decline. It is known that population size is an important element for the dynamics of natural forests through changes in vegetation structure and composition that need to be monitored. This study aims to analyze the species diversity in natural habitats of genetically diverse ebony, namely: i) Bantimurung National Park (BB), ii) Cani Sirenreng Nature Park (CS), iii) Farhumpenai Nature Reserve (FP), and iv) Pangi Binangga Nature Reserve (PB). Data collection was carried out based on a modified transect line and plot with 20 m x 100 m in size. The results showed that as many as 28 families were identified, consisting of 44 species at the tree level, 37 species at the pole level, 39 species at the sapling level, and 31 species at the seedling level, respectively. The composition of vegetation in Babul National Park consists of 32 species, Cani Sirenreng consists of 18 species, Farhumpenai consists of 19 species, and Pangi Binanngga consists of 19 species. The species composition was dominated by Diospyros celebica Bakh., Dracontomelon dao (Blanco) Merr. & Rolfe, Canangium odoratum, Ficus benjamina L., Pterospermum celebicum Miq., Kleinhovia hospita L. and Vitex cofassus Reinw. Ex Blume. The diversity index (H’-Index) of tree species in BB, CS, FP, and PB were 0.82, 1.13, 1.03, and 1.60, respectively. The Important Value Index (INP-Index) of ebony in BB, CS, FP and PB were 18.01%, 74.1%, 60.13% and 113.5%, respectively. The structure of the forest canopy layer in BB and FP consists of three layers of canopy, while CS and PB consist of two layers of canopy.

Identification of insects associated with ebony (Diospyros celebica Bakh.) as an endemic tree to Sulawesi

The population of ebony trees in Sulawesi has decreased and has been included in Appendix II Cites, which means it can only be traded based on quotas. Insect studies on ebony stands are still limited, especially in South Sulawesi. This study aims to determine the diversity and association of ebony with insects found in ebony stands in the Hasanuddin University Education Forest, Maros Regency. This activity was carried out in June 2021. Sampling was carried out using the light trap method for winged insects with nocturnal activity and pitfall traps for insects crawling on the ground. Data analysis to determine the diversity of insect species was carried out using the Shannon-Wiener (H') species and the Margalef index. The results showed that the insects found were 8 orders, 20 families, and 28 species from 128 individuals. Based on analysis of their functional role, the insect complexes consist of 34% herbivores, 50% predators, 8% parasitoids, 14% detritivores and 2% transient species. The diversity of species was classified as moderate, namely 2.71, while the richness index value of 5.56, which was classified as high richness in Ebony Stands.

Fungal diversity inhabiting tissues of ebony (Diospyros celebica Bakh.) in urban forest

Eboni (Diospyros celebica Bakh.) is an endemic tree species of Sulawesi Island including Central Sulawesi, West Sulawesi and South Sulawesi. This species is also called fancy wood; its color is black-striped reddish brown, beautiful, and luxurious. In addition, tree growth is influenced by microbes, including fungi. Fungi are heterotrophic and eukaryotic organisms absorbing organic compounds from other organisms. The study aimed to identify ebony-associated fungi in Urban Forestry at the Tamalanrea Campus, Hasanuddin University, Makassar, Indonesia. This study consisted of the isolation stage both direct and dilution methods, the rejuvenation stage, and fungal identification. The study result indicated that there were 60 fungal isolates isolated from the ebony tree tissues, while 19 fungal isolates were isolated on the soil under the ebony stands. The direct isolation-based method was higher in term of number of fungal isolates than the dilution-based method. The isolated fungi belonged to the seven genera, namely Aspergillus, Penicillium, Gliocladium, Trichoderma, Fusarium, Rhizopus, and Phytophthora. Aspergillus and Penicillium was genera dominated both in tree tissues and in the soil under ebony stands.

The dynamics of physiological properties of ebony (Diospyros celebica Bakh) based on crown position and altitude

Knowledge of the physiological properties of tree species is one of the basic requirements in determining the prescription silvicultural treatment of stands in nature. This study aims to analyze the physiological characteristics of the species of ebony (Diospyros celebica Bakh), as one of the potential endemic species of Sulawesi, based on leaf position in the crown and differences in altitude. Leaf sampling locations were in the Hasanuddin University Educational Forest (507 m asl) and Makassar city (16 m asl). The research variables consist of number of stomata, size of stomata, leaf stomata index, and leaf chlorophyll content. The results show that the physiological characteristics of the leaves were influenced by their position in the tree crown and differences in altitude. The highest number of stomata was found in the leaves exposed to the sunlights with 52.900 stomata/mm2 compared to 29.800 stomata/mm2 in shaded leaves. Likewise, the highest chlorophyll content was found in leaves exposed to the sunlights of 0.043 mg/L and shaded leaves of 0.034 mg/L. The difference occurs based on differences in altitude as well. The number of stomata in the lower area was 35.733 stomata/mm2, while in the high area was 42.667 stomata/mm2. The chlorophyll content in the low area was 0.038 mg/L and in the high area it was 0.037 mg/L. The higher a place, the more the number of stomata, this is inversely proportional to the size of the stomata and the stomata index, while the number of chlorophylls is not significantly different.

Discrimination and Determination of Extractive Content of Ebony (Diospyros celebica Bakh.) from Celebes Island by Near-Infrared Spectroscopy

Ebony (Diospyros celebica Bakh.) is an endemic plant on Celebes (Sulawesi) island. Extractive compounds within ebony wood cause it to have durability, strength, and beautiful patterns. In this study, we used near-infrared (NIR) spectroscopy to discriminate between ebony wood samples, based on their origins at different growth sites on Celebes island, and to develop quantitative models to predict the extractive content of ebony wood. A total of 45 wood meal samples from 11 sites located in West, Central, and South Celebes were collected in this study. NIR spectral data were acquired from hot water and ethanol–benzene soluble extracts from ebony wood in this study. The extractive content of the ebony was 10.408% and 10.774% based on hot water solubility and treatment with ethanol–benzene solvent, respectively. Multivariate analysis based on principal component analysis–discriminant analysis revealed that ebony wood from West Celebes differed from most of the wood from South Celebes; however, it was only slightly different from ebony wood from Central Celebes based on NIR spectra data. These findings were in line with the extractive contents obtained. Partial least square regression models based on wood meal spectra could potentially be used to estimate the hot water and ethanol–benzene extractive contents from ebony wood.