High-yielding wild species of yam (Dioscorea spp.) play a vital role in the diet, nutritional, and economic stability of the tribal community. Lack of in-depth research provides scant information on the nutritional value and quality characteristics of tubers. In the present study, physicochemical, nutraceutical, antinutrients, structural, morphological, and thermal properties of lesser-known yam species: Dioscorea pentaphylla (five-leaf yam), Dioscorea bulbifera (bitter yam), Dioscorea hispida (intoxicating yam), and Dioscorea tomentosa (locally called ‘targai kanda’) were investigated. Total protein, fat, fiber, ash, carbohydrate, and metabolic energy of the investigated species are 5.05–8.79%, 0.33–1.01%, 3.53–14.15%, 3.30–5.31%, 62.60–78.26%, and 1241–149 kJ/100 g, respectively. Significant variations (P ≤ 0.05) were observed among the species for antioxidant, bioactive compounds, and physicochemical attributes, where Dioscorea tomentosa exhibited the highest antioxidant capacity. The starch and amylose content varied between 24.7–57.8% and 12.7–29%. X-ray diffractograms of D. pentaphylla and D. bulbifera displayed a B-type pattern, A-type starches in Dioscorea hispida, and CB–type starch for D. tomentosa. The FT-IR spectra confirmed the polysaccharide nature of the yam flours. The particle size ranged between 9.6 and 72.4 μm. The TG/DTG curves revealed that the yam flour could be subjected to cooking up to 180 °C without carbonization. The reported wild yam flours may have tremendous potential for industrial application. However, the study revealed that the presence of antinutrients might affect the mineral bioavailability, cause toxicity, and health hazards. Thus, this could be overcome by processing before safe consumption. The current study also recommended that these wild yam species are safe and can meet potential nutrition and food security through mainstream cultivation.
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