Dinoflagellate Cysts Research Articles

The Scriniodinium crystallinum dinoflagellate cyst zone in the Middle–Upper Oxfordian, Upper Jurassic, Ilimananngip Nunaa (Milne Land), East Greenland

The biostratigraphy of the Jurassic in East Greenland is historically based on macroscopic fossils. Stratigraphy based on palynomorphs (spores, pollen and dinoflagellate cysts) has progressed more slowly and sporadically. The Scriniodinium crystallinum dinoflagellate cyst Zone is identified in middle – upper Oxfordian strata of Ilimananngip Nunaa (Milne Land), central East Greenland. The lower boundary is defined by the last occurrence of Trichodinium scarburghense in the Cardioceras tenuiserratum ammonite Zone. The upper boundary is defined by the last occurrence of S. crystallinum in the uppermost Amoeboceras rosenkrantzi ammonite Zone. However, the subzonal division of the S. crystallinum Zone recorded in North-West Europe is not identified in Greenland. Eighteen characteristic dinoflagellate cyst events are considered stratigraphically significant and useful in East Greenland. Fifteen of these events provide an informal, detailed stratigraphical subdivision of the S. crystallinum Zone into 10 subunits. Identification of the zone is an addition to the previously defined upper Bathonian – middle Oxfordian zonation, where the uppermost palynostratigraphical event was recorded to be the last occurrence of T. scarburghense. With this study, the correlation of dinoflagellate cyst and ammonite stratigraphy in the lower and middle Oxfordian is slightly modified. The S. crystallinum Zone documented here, in combination with the zonation used for the stratigraphy of the Blokelv-1, Rødryggen-1 and Brorson Halvø-1 cores of the Upper Jurassic to Lower Cretaceous, completes the dinoflagellate cyst stratigraphy of the marine Jurassic in East Greenland. Together with previous studies of spores and pollen in less marine units, the first complete palynological Jurassic stratigraphy is thus established for the Jurassic succession in East Greenland.

Organic walled dinoflagellate cyst biostratigraphy of the Bartonian/Priabonian GSSP Alano di Piave section, NE Italy

The present paper augments the multidisciplinary stratigraphic analysis of the Global Stratotype Section and Point (GSSP) Bartonian-Priabonian boundary Alano di Piave section (NE Italy). Twenty-seven stratigraphically signficant organic walled dinoflagellate cyst events, calibrated against the magnetostratigraphic polarity timescale, and earlier established zonations based on calcareous microfossils are recognized. Among these events the first occurrence (FO) of Oligokolpoma agniniae sp. nov. (~8 m below the GSSP boundary), and the FO of Reticulatosphaera actinocoronata (~8 m above the GSSP boundary) may be considered as additional stratigraphic indicators for the Bartonian-Priabonian boundary. Six new potentially stratigraphically significant species Heteraulacacysta alanoensis sp. nov., Heteraulacacysta reticulata sp. nov., Impagidinium bellunoensis sp. nov., Impagidinium veneziaense sp. nov., Lophocysta domenicorioi sp. nov., and Oligokolpoma agniniae sp. nov. are formally described.

Sedimentary organic matter and paleoenvironmental reconstruction of the Santana Group (Lower Cretaceous), Araripe Basin, Northeast Brazil

Depositional controls of the Santana Group in the Araripe Basin are still being debated. The main controversial subject is their marine influence and paleoenvironmental evolution. In this study, palynofacies analysis was performed on 59 samples from one core drilled at Sítio Romualdo in the Araripe Basin to investigate sedimentary organic matter. Three palynofacies associations (structureless, continental particles, and aquatic + opaque) were identified. The Santana Group consist of Barbalha, Crato, Ipubi and Romualdo formations. Based on palynofacies associations we conclude that the depositional setting of the Barbalha Formation reflects a fluvially-fed lacustrine system under oxic conditions. The Crato Formation, associated with tidal flats, coastal lakes, and lagoons within an open bay environment, represents a bayhead delta system. The Ipubi Formation corresponds to a coastal plain typical of sabkha paleoenvironments, characterized by low energy under reducing conditions, during drier climate conditions. The Romualdo Formation records mainly an estuarine paleoenvironment characterized by dysoxic to anoxic conditions. The presence of palynomorph groups typifies a late Aptian age for the Santana Group, which is based on the range of guide species marked mainly by the presence of Sergipea variverrucata biozone (P-270), as well as the occurrence of Complicatisaccus cearensis and Equisetosporites maculosus. The identification of marine palynomorphs (dinoflagellate cysts Subtilisphaera and foraminiferal test linings) are indicative of several, local marine incursions.

Neogene–Quaternary paleoenvironments and kerogen assessment of the NDO B-1 well, offshore Nile Delta, Egypt, Eastern Mediterranean: palynological evidence

Palynofacies and palynological investigations conducted on the Neogene–Quaternary succession from the NDO B-1 well, located in the offshore Nile Delta, Egypt, in the Eastern Mediterranean, suggest generally shallow marine (neritic) conditions. These environments are manifested by the overall palynofacies composition and the occurrence of dinoflagellate cysts (e.g., Spiniferites spp., Lingulodinium spp., Hystrichokolpoma spp., Homotryblium spp. and Selenopemphix spp.). Neritic environments are suggested for the lower and middle Miocene Sidi Salim, and the Pliocene to Pleistocene upper Kafr El Sheikh, El Wastani and Mit Ghamr formations, while shallower, coastal to inner neritic settings were interpreted for the late Miocene (Qawasim and Rosetta formations) and early Pliocene (Abu Madi and lower Kafr El Sheikh formations). Anoxic conditions existed during the deposition of the studied well succession, which can be seen from the occurrence of imprints of pyrite crystals and some types of oxygen-sensitive dinoflagellate cysts. The palynofacies fluctuated repeatedly between Amorphous Organic Matter (AOM)-dominated and phytoclast-dominated intervals, of kerogen types II and III, respectively. The spore coloration index (SCI) of indigenous thin-walled palynomorphs confirms thermally mature sediments, generative of dry gas and wet gas/condensates. Reworking during the deposition of the upper Sidi Salim, Qawasim and Rosetta formations is inferred from the occurrence of Cretaceous dinoflagellate cysts and pollen.

Environmental changes in the Fleuve Manche paleoriver drainage system (Western Europe) linked to North Atlantic sub-millennial climate variability across Heinrich Stadial 1: Palynological evidence from the Bay of Biscay

Marine microfossils (dinoflagellate cysts and planktonic foraminifera) and geochemical (XRF-Ti/Ca)-based climatic records from a core (MD13–3438) located off the Fleuve Manche (FM) paleo-mouth have revealed that sustained warm summer sea surface temperatures (SSTs) during sub-millennial climate changes within HS1 (∼18–14.7 ka) may have played a key role in the FM regime related to the European Ice Sheet (EIS) melting rate. In this study, we have analyzed the MD13–3438 pollen content over the HS1 at a mean resolution of ∼50 years to test whether vegetation-based air temperatures were coupled to SSTs face to this rapid climate variability. First, our results highlight two major phases of pollen sources at site MD13–3438, preventing the pollen record to be interpreted as a continuous record of the evolution of vegetation and climate occupying a single watershed across HS1. The first phase, i.e. the HS1-a interval (∼18–16.8 ka), is marked by strong occurrences of boreal pollen taxa (especially Picea-Abies). Considering their spatial distribution and the coalescence of the British and Scandinavian ice sheets into the North Sea during the Last Glacial Maximum, these taxa probably originated from the North European Plain, i.e., eastern FM tributaries (east of the Rhine River), where cool-humid conditions generally prevailed. Then, the second phase, i.e. the HS1-b interval (∼16.8–14.7 ka BP), is characterized by a deceleration of the EIS retreat and the drop of boreal pollen values at site MD13–3438 further signing a less influence of the upstream FM drainage system and thus a better characterization of pollen sources related with western FM tributaries. Superimposed to these two HS1 main phases, pollen fluctuations are concomitant with sub-millennial variability in the EIS deglaciation intensity. During the early HS1 (HS1-a), we discuss two short-term increases in the ratio between deciduous trees (Quercus-Corylus-Alnus) and herbaceous plants (Plantago-Amaranthaceae-Artemisia). These events are coeval with phases of increasing dinocyst-based SST seasonality (i.e. through summer SST amplification). We associate these events with lower contribution of the upstream FM catchment as well as, possibly, atmospheric warming and regional sea-level positive oscillations. The HS1-b is composed of three main phases that appear more influenced by the downstream FM drainage system. HS1-b1 (16.8–16.3 ka BP) corresponds to the driest and coldest conditions west of the Rhine River. HS1-b2 (16.3–15.5 ka BP) is coeval with large arrivals of iceberg from the Hudson strait in the Bay of Biscay and thus likely to a major sea-level positive oscillation associated with a phase of FM valley reworking. HS1-b3 (15.5–14.7 ka BP) corresponds to persistent arid conditions that preceded the subsequent more humid conditions recorded from 14.7 ka BP at the start of the Bölling-Alleröd.

Pollen evidence of enhanced Yellow River provenance into the South Yellow Sea after the Middle Pleistocene Transition

Quaternary sediment of the South Yellow Sea (SYS) is usually assumed to record climatic changes, tectonic activities and major river evolutions. However, as an important terrigenous indicator, a long sequence of pollen proxy in the SYS lacks for studies of river provenance and climate changes. In this study, we focused on a 150-m pollen record in the SYS that covered most of the Quaternary period over the past 1.8 Ma. The most significant changes of pollen assemblage were caused by river provenance, that high pollen percentages of broadleaved trees such as Castanea/Castanopsis reflect a dominant provenance of the Yangtze River since ca. 1.8 Ma, with a decline trend during the Mid-Pleistocene transition (MPT) mainly between 1.1 and 0.8 Ma. The sharp increase in coniferous pollen, particularly Picea, originated from the Yellow River drainage areas, suggesting a major alteration in terrigenous pollen source from the Yangtze River to the Yellow River at ∼0.8 Ma, which agrees well with the timing of the final integration of the modern Yellow River. The high pollen quantities of Artemisia and Ephedra also shows the enhanced Yellow River provenance where most landscape was covered by semi-arid open vegetation (e.g. steppe). The alternate appearances of marine foraminifera and dinoflagellate cysts, and freshwater wetland plants (Cyperaceae, Poaceae) through the interglacial intervals from MIS 19/21 demonstrate different estuary sedimentary environments and diverse salinity, associated with the sea-level fluctuations. The pollen concentration suggests an increase in terrigenous materials towards the interglacial MIS 11 and the preliminary delta formation of the Yellow River. We propose that the terrigenous input from the Yellow River accelerated after the MPT, and combined effects of tectonic movement drove the evolution of the river delta plains in eastern China. Our data also reveal some secondary periodic changes in evergreen broadleaved forest during the past 1.8 Ma and cold-tolerant coniferous forest since particularly the MPT, which are generally linked with the 400-ka long eccentricity cycle and the variability of monsoon climate.

Two areoligeracean dinoflagellate cysts from the Carstone Formation (Lower Cretaceous) at Middlegate Quarry, North Lincolnshire, UK

Five Lower Albian (Lower Cretaceous) areoligeracean dinoflagellate cyst species are present in the Carstone Formation at Middlegate Quarry, North Lincolnshire, UK: Canninginopsis monile , Circulodinium deflandrei , Cyclonephelium compactum , Cyclonephelium intonsum and Cyclonephelium longispinatum . The taxonomy of Circulodinium deflandrei is reviewed with respect to 20 measured Carstone specimens. The taxonomy of Cyclonephelium longispinatum is reviewed with respect to 70 measured Carstone specimens and a study of 40 measured specimens from its type material in Site 361, Deep Sea Drilling Project Leg 40, offshore southwestern Africa. Circulodinium deflandrei and Cyclonephelium longispinatum have recently been considered by other workers to be junior synonyms, respectively, of Circulodinium hirtellum and Tenua anaphrissa . However, following detailed study of their morphologies, the synonymies are formally rejected here. An emended diagnosis is given for C. longispinatum . A re-assessment of the stratigraphical ranges of taxa co-occurring with the type material of C. longispinatum is consistent with a Late Albian to Early Turonian age range in Site 361.

Late Pleistocene–Holocene palynology and paleoceanography of İmralı Basin, Marmara Sea: Pollen-spore, dinoflagellate cyst and other NPP zonations

The Marmara Sea is the oceanographic gateway that links the hypersaline Eastern Mediterranean Sea with the hyposaline Black Sea via Straits of Dardanelles and Bosphorus, respectively, and also occupies a transitional vegetation and climatic regime between the Mediterranean and Euxinic phytogeographic ecosystems. Recent palynological studies of long cores from the shallow İmralı Basin perched on the southeastern slope of the Marmara Sea reveal that major paleoecological changes accompanied the isolation and reconnection of the Marmara Sea during the last two Pleistocene glacial–interglacial cycles. Here, we use data primarily from two İmralı Basin cores to determine new quantitative zonations for the pollen-spore, dinoflagellate cyst (dinocyst) and other non-pollen palynomorph (NPP) assemblages in sediments representing the last ∼30 cal ka. A gap in one core is closed using data from three short gravity cores. Twenty-four radiocarbon ages and 20 correlation-ties for sapropel and ash layers from these five cores provide an essentially complete record for the past 30 cal ka. Use of a full suite of palynomorph data (pollen and spores, fungal spores, dinocysts and other phytoplankton, aquatic parasites/saprophytes, zoo-planktonic and zoo-benthic NPPs) shows for the first time that fluctuations in aquatic palynoassemblages during the glacial periods are more complex than simple salinity or temperature responses. The changes involve food web-level turnovers in trait- and size-based food web components in which potential parasites/saprophytes appear critically important.Pollen stratigraphies reveal a significant loss of Euxinic tree taxa by MIS 3c, with only partial recovery in MIS 1. The Euxinic forest elements are replaced by cultivars and other indicators of anthropogenic influences during the MIS 1 that is also marked by a dry interval and corresponding in part with the 4.2 cal ka Eastern Mediterranean Drought Event. The dinocyst zones for the last ∼30 cal ka show a clear turnover from assemblages dominated by brackish Ponto–Caspian taxa to marine assemblages at ∼11.7 cal ka. This phytoplanktonic signal lags slightly behind (a) the vegetation and shift in the other NPP commencing ∼13.2 cal ka, the initial marine incursion at 13.8 cal ka, and the arrival of coccolithophores at ∼13 cal ka. Comparison to published MIS 6 and 5 data reveals higher MIS 2–1 species diversity, reappearance of the MIS 6 marker Impagidinium inaequalis, and the emergences of Pyxidinopsis psilata f. quadrata, cf. Boreadinium breve, and Peridinium spp. of Brenner 2005. The pre-reconnection microbiota is dominated by the unicellular alga Sigmopollis, spikes of colonial Pediastrum algae, the saprophytes/parasites cf. Multiplicisphaeridium and Chytridiomycota. This microbiota is displaced by the post-reconnection assemblage dominated by Botryococcus coenobia, various Platyhelminth egg capsules and ostracod lining-types that persist through the Holocene. A distinctive transitional microbiota with Discocotyle-type turbellarian egg capsules marks the Bølling–Allerød warming, followed at ∼13.3 ka by a marine assemblage with micro-foraminiferal linings and crustacean eggs. The interglacial marine phase of MIS 1 in İmralı Basin has much higher dinocyst species diversity than MIS 5e, reflecting Neolithic impacts (agriculture, land clearance) and expanded shipping trade. Initial marine flooding in the Marmara Sea during both interglacials are marked by sapropel deposition but variations of ravinement surface erosion, and marine deep water residence time in a stratified Marmara Sea are potentially important factors underlying hypoxia/anoxia and sapropel deposition.

Geographic distribution of modern dinoflagellate cysts in surface sediments of the Arabian Sea: significance of taxa and morphometry as potential ecological indicators.

Studies on dinoflagellate cysts in the Arabian Sea (AS) are limited to the coastal waters, but no information from the deeper depths. The dinoflagellate cyst assemblages in surface sediment samples (0-2cm) from the deeper depths (up to ~ 4500m) of central (oxygen minimum zone (OMZ)) and southeastern (oligotrophic) AS revealed that the relatively good numbers of cyst concentrations reach deeper depths of OMZ (3505m) and oligotrophic (4368m) regions, but the former harbored more cyst concentrations than the latter. The cyst concentration and species count (including HAB species) recorded here are lower compared to the eastern (EAS) and western (WAS) AS, but the autotrophic cyst dominance (74-83%) at deeper depths is in contrast with the heterotrophic dominance in coastal AS. Of the recorded 41 cyst species (belonging to 18 genera), four species (cyst of Cladopyxis sp., Operculodinium janduchenei, Stelladinium bifurcatum, and Protoperidinium monospinum) from the deepest part of oligotrophic AS form the first report. In contrast, Spiniferites and Lingulodinium cysts were common occurrences. Taxonomic comparison with literature revealed (i) the prevalence of more cosmopolitan species (32 species) which could be due to the prevalence of large and small-scale lateral transport of cysts in oligotrophic regions followed by OMZ and coastal regions, respectively, and (ii) very few region-specific species, i.e., cyst of Protoperidinium latissimum, Lejeunecysta sabrina, cyst of Protoperidinium denticulatum in EAS and Impagidinium patulum, and I. strialatum, in WAS. Interestingly, variability in the morphometry was evident between the coastal and open oceans in some cosmopolitan cysts, e.g., Operculodinium centrocarpum and Lingulodinium machaerophorum. These findings from the less studied pelagic regions will contribute to the growing knowledge of dinoflagellate cyst distribution patterns and highlight the significance of cyst taxa and morphology as potential ecological indicators for AS.

Evolution of West‐East Contrast in the Subarctic Pacific Gyre During the Plio‐Pleistocene Based on Palynological Evidence at ODP Sites 882 and 887

AbstractFragmentary and contradictory evidence prevents a clear understanding of the possible role of the North Pacific Ocean surface on the ice‐sheet evolution. New palynological data encompassing the Pliocene and Pleistocene at Ocean Drilling Program Site 882 in the northwest Pacific document sea‐surface conditions from dinoflagellate cysts in addition to atmospheric trajectories from pollen grains and spores. The comparison of the Site 882 record with that of Site 887 from the northeast Pacific also permits documenting West to East contrasts across the subarctic North Pacific. The dinocyst assemblages at the two sites indicate strong temperature contrasts until 4.2 Ma, with much warmer conditions in the East. From 4.2 to 3.6 Ma, dinocyst assemblages of both sites are characterized by alternated dominance of the extinct taxa Habibacysta tectata and Impagidinium detroitense suggesting homogenous and cool conditions across the subarctic Pacific gyre. A major transition is recorded in the palynological assemblages at Site 882 around 2.7 Ma. It is marked by the highest occurrence of Habibacysta tectata and high percentages of Filisphaera microornata additionally to thermophilic taxa. Dinocyst record indicates high seasonality and warmer surface conditions due to reinforced stratification, which support the hypothesis of high evaporation at the origin of atmospheric moisture supply to high latitudes. During the last 1.2 Ma, the increase of Operculodinium centrocarpum relative to Nematosphaeropsis labyrinthus is tentatively interpreted as a reinforced influence of the subtropical waters. Hence, we suggest that the warm surface ocean fostered the inception and growth of northwestern North American ice‐sheets during the Pliocene and Pleistocene.

First insights into the distribution and diversity of toxic dinoflagellate cysts in the surface sediments of Dakhla Bay (African Atlantic coast): relationships with environmental factors and mollusk intoxication events.

Dakhla Bay, situated on the African Atlantic coast, has witnessed sporadic harmful algal blooms (HABs) caused by toxic dinoflagellate species over the past two decades. In this study, we investigated the distribution, abundance, and diversity of dinoflagellate cysts, with a focus on potentially toxic species that develop in this ecosystem where such data are lacking. Sediment samples were collected in April 2018 through coring at 49 stations distributed across the bay. The highest abundance of dinoflagellate cysts was recorded at 304 cysts/g dry sediment, observed at the inner part of the bay, indicating that this area is the preferential zone for cyst accumulation. Pearson's tests revealed significant positive correlations (P < 0.05) between cyst abundance and the water content, organic matter, and fine fraction (< 63μm) of the sediment. Cyst morphotypes of potentially toxic dinoflagellate species known to produce saxitoxins, such as Alexandrium minutum, Alexandrium tamarense species complex, Gymnodinium catenatum, and yessotoxins, such as Lingulodinium polyedrum and Gonyaulax cf. spinifera, were identified in the sediment of Dakhla Bay. These findings were further supported by our long-term monitoring period (2005-2018), underscoring the presence of these HAB species in Dakhla Bay. During our survey, sporadic mollusk intoxication events were recorded at station PK25 for the grooved razor shell Solen marginatus and at station Boutelha for the oyster Crassostrea gigas. Paralytic shellfish toxin concentrations exceeded the sanitary threshold (80μg STX di-HCl eq/100g of shellfish meat) only twice, in December 2006 and January 2007 at station PK25. Contamination by amnesic shellfish toxins occurred sporadically but never reached the sanitary threshold of 20µg/g of shellfish meat. Lipophilic shellfish intoxication occurred multiple times in the two investigated areas. These observations suggest that the cysts of the identified HAB species germinated and inoculated the water column, resulting in the observed intoxication events. Relatively low levels of intoxication could be attributed to the moderate abundances of cysts, which may reduce the seeding capacity. This could be explained by the significant interaction of Dakhla Bay with the Atlantic Ocean, characterized by hydrological dynamics that impede the deposition and accumulation of cysts in the bay's sediments. This study reaffirms the importance of investigating dinoflagellate cysts in assessing the diversity of HAB species and evaluating associated sanitary risks.

Climate variations in eastern Hudson bay over the past 3000 years

Palynological analyses of the marine sediment core AMD0509-20 collected north of Whapmagoostui-Kuujjuarapik in Nunavik were performed to investigate past changes in climate and sea-surface conditions based on pollen and spores and dinoflagellate cysts, respectively. Our results show relatively warm sea-surface temperatures before 2600 BP, about 2 °C higher than at present in summer, in addition to higher salinity suggesting relatively low freshwater inputs, thus low precipitation over the watershed. After 2400 years BP, an abrupt drop in sea-surface temperatures was recorded, followed by a decrease in salinity particularly pronounced until ∼2000 years BP. After 1600 years BP, summer sea-surface temperatures and salinity stabilized around current values. The changes recorded in surface waters during the late Holocene can be associated with a regional shift in the hydroclimatic regime that led to a gradual decline of spruce (Picea) in pollen assemblages.

Environmental factors influencing the abundance and spatial distribution of modern dinoflagellate cysts in Izmir Bay (Eastern Mediterranean)

We analyzed surface sediments from 12 stations located in Izmir Bay to determine the impact of anthropogenic pollution on dinoflagellate cysts. Forty-three dinoflagellate cyst taxa and two cyst assemblage zones were identified. Zone 1 is characterized by the dominance of cysts of Gymnodinium nolleri, Selenopemphix nephroides, and Operculodinium centrocarpum from the oligotrophic part of Izmir Bay. Zone 2 is in the highly productive inner part of the bay and is characterized by the high abundances of Lingulodinium machaerophorum, Spiniferites ramosus, cysts of Scrippsiella spp., cysts of Polykrikos spp. and Quinquecuspis concreta. We used multivariate statistical analysis (DCA and CCA) on dinoflagellate cysts and environmental variables to support the identification of Zones 1 and 2. Our analyses also revealed that summer and winter chlorophyll-a as well as elevated nitrate and nitrite concentrations are significant parameters in controlling dinoflagellate cyst distribution in Izmir Bay.

Palynostratigraphy and Paleoenvironment of Afikpo Well_3, Afikpo Syncline, Nigeria

The Afikpo Syncline constitutes the western segment of the southern part of the Benue Trough. The syncline orients in the northeast-southwest direction and unconformably overlies the pre-Santonian strata of the Benue Trough. This study investigates the sediments of Afikpo Well_3, drilled within the syncline, identifies its different palynozones, and interprets their paleoenvironments of deposition. Forty-two ditch-cutting samples recovered from the well at a 150 ft interval consist of mudstones, calcareous mudstones, carbonaceous shales, and calcareous shales. These samples were subjected to palynological analysis following the conventional acid maceration technique. A total of 637 palynomorph counts were recovered. These comprise 316 pollen grains, which make up 49.61%; 129 spores, which make up 20.25%; 9 fungi spores, which make up 1.41%; 17 microforaminifera wall linings, which make up 2.67%; and 166 dinoflagellate cysts, which make up 26.06%. Two palynozones were identified within the well. These include zone AF-01 (Monocolpites marginatus assemblage zone), which ranges from depths of 1500–1320 ft and is Campanian in age; and zone AF-2 (Dinogymnium sp. assemblage zone), which extends from depths of 50 ft–1320 ft and dates back to the Maastrichtian period. Based on the predominance of palmae pollen, together with the subordinate occurrence of dinoflagellate cysts, and the fair representations of spores (Cyathidite minor), the environment of deposition of the sediments ranges from a nearshore brackish water to a shallow marine environment. The shallow environment is further confirmed by the frequent occurrence of microforaminifera wall lining and the dominance of the peridinoid dinoflagellate cysts over the gonyaulacinoids.

The Cretaceous to Eocene: a biostratigraphical review and a new detailed palynostratigraphy of Greenland and adjacent areas

The present paper compile and correlate for the first time Cretaceous to Eocene palynostratigraphies across the Arctic. It focus on Greenland and adjacent areas, including the Labrador–Baffin Seaway, onshore Nuussuaq Basin in central West Greenland, onshore southern East Greenland, central East Greenland, North-East Greenland, eastern North Greenland and the Danmarkshavn Basin, but also extends to Canadian Arctic Archipelago and the Barents Sea region off Norway. The paper compile data from more than three decades of detailed Arctic palynological analyses, based mainly on dinoflagellate cysts. The paper gives a historical overview of the Cretaceous to Palaeogene paleontological studies of Greenland and presents an overview of 85 palynological intervals and numerous events. The palynological assemblages from the Labrador–Baffin Seaway, Nuussuaq Basin and north-east Baffin Bay reflect the opening of the Labrador–Baffin Seaway from brackish to freshwater environment in a large embayment in the Early Cretaceous to an open marine seaway in the Late Cretaceous. Assemblages reveal in dinoflagellate cyst provincialism between the opening stages of the Labrador–Baffin Seaway and the already opened Greenland–Norwegian–Barents seaway. The Upper Cretaceous global Oceanic Anoxic Event 2 (OAE2) spanning the Cenomanian/Turonian boundary is recognised from Arctic Canada, north-east Baffin Bay, Nuussuaq Basin in central West Greenland, and North-East Greenland, and is mapped and correlated based on dinoflagellate cyst stratigraphy and carbon isotope (δ13Corg) curves. The dinoflagellate cysts assemblages of the Cretaceous/Palaeogene boundary are correlated from the Labrador Sea across to the Nuussuaq Basin in central West Greenland; in both areas the earliest Danian palynological assemblage is represented by incoming warm-water species. The presence of the global Paleocene–Eocene Thermal Maximum (PETM) in the Palaeogene successions in North-East Greenland and in exploration wells in the Labrador–Baffin Seaway is indicated by the incoming of the warm-water dinoflagellate cyst species Axiodinium augustum.

PALYNOLOGICAL, PALEOENVIRONMENTAL AND PALEOCLIMATE ANALYSES OF EMI-5-WELL, OFFSHORE NIGER DELTA, NIGERIA

Palynological, Paleoenvironmental and Paleoclimatic investigations of Emi-5 well Offshore, Niger Delta, Nigeria was conducted to established palynozones, determine age, reconstruct paleoenvironmental conditions and paleoclimatic conditions to permit stratigraphic correlation, provide insights into the depositional settings and changes overtime within the basin. Fifty one (51) shaly ditch cutting samples were subjected to standard palynological method involving sample maceration with 10 % HCl and 40 % HF acids to remove carbonates and siliceous components, neutralization with distilled water and sieving through 5-micron sieve, followed by separation of palynomorphs from residue prior to mounting of the grains on glass slides for further analysis. The palynological composition is marked by dorminance of pollen and spores over dinoflagellate cysts. The intercalation of the sandy and shaly intervals across the section evidenced from lithologic, textural, and wire line log data suggested that the entire studied interval belong to the Agbada Formation. Three interval range zones were delineated using the international stratigraphic guide for the establishment of biozones, they are Stereisporites sp – Verrutricolporites rotundiporus; Verrutricolporites rotundiporus – Gemmemonoporites sp. and Retistephanocolpites gracilis – Pachydermites diederixi Zones and based on the aforementioned zones the interval is assigned middle Miocene to late Miocene. Coastal-deltaic environment of deposition is inferred for the studied interval on the basis of occurrence and dominance of diagnostic forms such as Zonocostites ramonae,Rhizophora, Psilatricoloporites crasssus and Acrostichum aureum while good representation of these forms and the identified palyno-ecological groups placed the interval on a wet climatic zone.

Labrador Coastal Current and productivity variations offshore Nain (Nunatsiavut) during the late Holocene

The Labrador Coastal Current (LCC) transports cold and fresh waters of Arctic origin towards the North Atlantic and has a strong influence on the coastal ecosystems and climate of eastern Canada. Here, we present a multi-decadally resolved record of changes in sea-surface and near-bottom conditions of the LCC during the last 3000 years based on the analysis of biogenic tracers (organic-walled dinoflagellate cysts, carbon, and nitrogen geochemistry) and sedimentary content (sortable silt, magnetic susceptibility, elemental ratios) in the upper 2 m of sediments in a core retrieved offshore Nain, Nunatsiavut. Our data reveal multi-centennial bottom flow strength variability in the LCC, with strong linkages to changes in regional ocean circulation patterns. At about 2100- and 550-years BP, the sedimentological and biogenic tracer data suggest changes in the LCC bottom current intensity (decline and strengthening, respectively) that coincide with shifts in the persistent mode of the North Atlantic Oscillation. Our proxy data suggests increased biological production during the last 500 years compared to the preceding 2500 years, possibly reflecting a gradual decrease length of the sea-ice season. The data show a decline in the LCC strength during periods of increased polar water influence identified in previous palaeoceanographic studies conducted further north, which may be attributed to the trapping of freshwater in sea ice during these periods. Our results highlight the dynamical oceanographic and atmospheric factors that influence the LCC and the coastal ecosystems along its path and confirm that the processes in Baffin Bay impact the Labrador Shelf oceanography.

Application of palynomorphs and palynofacies in Early Cretaceous paleoenvironmental reconstruction; Shushan Basin, Egypt

An integration of palynomorph and palynofacies data from the Shushan-1X well is used to infer the paleoenvironmental conditions of the Valanginian to Middle Cenomanian (Cretaceous) section of the western Shushan Basin, northern Egypt. The data obtained contribute significantly to the depositional history of the basin. The low diversity of dinoflagellate cyst assemblages, along with the dominance of land-derived spores and pollen, suggest restricted (marginal) marine environments, in contrast to their coeval representatives from the Tethyan Realm. Open marine (inner shelf) environments developed at a few horizons in the Dahab and Bahariya formations, partly contemporary with the global Aptian and Cenomanian eustatic cycles. These environments were relatively more offshore than those described in the eastern and southeastern parts of the basin. The study of total palynological organic matter (TPOM) has contributed largely to these established environmental settings. It has also allowed the recognition of redox (suboxic to anoxic) conditions and the impact of a large magnitude of terrigenous influence.

Study on the diversity and germination of dinoflagellate cysts in the sediments of foreign ships in Shanghai Port

The dinoflagellate cysts present in the ballast water sediment of foreign ships in Shanghai Port have not been previously studied. Therefore, sediment samples were collected from the ballast water of 16 foreign ships in Shanghai Port, and the types of dinoflagellate cysts were identified and their abundance was calculated, with a specific focus on the analysis of toxic and harmful dinoflagellates. Moreover, simulations of temperature and salinity conditions throughout the year in the Shanghai port waters were conducted to carry out dinoflagellate cyst germination experiments, with analyze and compare the germinated dinoflagellate cysts under different conditions. Dinoflagellate cysts were found in 100 % of the ship sediment samples, including a total of 9 species of toxic and harmful dinoflagellate cysts. In the germination experiment, 15 °C was found to be the optimal temperature for the germination of dinoflagellate cysts in ballast water sediment, and high salinity is more favorable for cyst germination.

A record of late Holocene sea level and human impacts from the southeastern Coast of Sri Lanka

AMS radiocarbon-dated palynological evidence from Embilikala Lagoon in Bundala National Park (one of the Ramsar wetland sites) on the southeast coast of Sri Lanka indicates palaeoenvironmental changes during the last 2,700 years. The persistence of fewer mangroves (eg Rhizophoraceae taxa) suggests that sea level low stands occurred between 2,700 and 1750 cal yr BP. From the later age, the relative sea level rose, and reached the present sea level at 950 cal yr BP as indicated by the mangrove maximum. At 2,250, 700–400 and 200–150 cal yr BP, the sudden increase in marine dinoflagellate cysts (eg Spiniferites mirabilis) and foraminifera (Globigerinoides ruber) suggests increased marine influence. This is consistent with a decrease in mangrove habitats. Between 2,700 and 950 cal yr BP, severely degraded dry monsoon forests and minimal levels mangrove suggest human and marine influences were the critical drivers for changing the major vegetation types along the southeastern coast. Vegetation changes have been drastic over the last millennia.