Endurance performance is impacted by physiological, anthropometrical, diet, genetic, psychological, and training characteristics. Altitude can affect physiological parameters (like maximum oxygen utilization, arterial oxygen saturation (SaO<inf>2</inf>), heart rate, and blood pressure) and training characteristics (duration, frequency, and training load). Thus, this study compared physiological parameters and training characteristics between endurance runners at Ethiopian Youth Sports Academy (EYSA) and Guna Athletics Sport Club (GASC) located which are located at elevation of 2400 and 3100 meters, respectively. A comparative cross-sectional study design was used from September to November 2021. Data was obtained from a total of 120 eligible participants (30 runners and 30 controls at GASC, and 30 runners and 30 controls at EYSA). Sociodemographic and training characteristics were assessed using self-administered standardized questionnaires. SaO<inf>2</inf> and heart rate were measured using finger pulse-oximetry (Nellcor, Oxim N-65; Covidien, Dublin, Ireland). Blood pressure was measured by Folee Digital Blood Pressure Monitor DX-B1 (Jiangsu Folee Medical Equipment Co., Ltd., Zhenjiang, China). The runners of GASC had significantly lower resting SaO<inf>2</inf>, SaO<inf>2</inf> immediately after maximal exertion, training (frequency and load) than runners of EYSA in both sexes. Runners of Guna Athletics Sport Club (RGASC) had significantly higher maximum oxygen utilization (VO<inf>2</inf>max) than Runners Ethiopian Youth Sports Academy (REYSA) in both sexes. Severe exercise-induced hypoxemia (EIAH) and moderate EIAH were developed by male runners of GASC and EYSA respectively. Besides, female runners GASC and EYSA developed moderate and mild EIAH, respectively. Conclusively, there were significant differences in physiological parameters and training load between RGASC and REYSA. High altitude (3100 meters) may have a greater impact on the above variables than moderate hypoxia (2400 meters above sea level). Hence, endurance runner coaches should give emphasis to these variables.
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