Simple SummaryArabinoxylans (AXs) constitute the major non-starch polysaccharides (NSPs) existent in maize and soybean meal, comprising about 52% and 65% of the total NSP. Previous works have illustrated that the incorporation of arabinofuranosidase (Abf; GH51) plus xylanase (Xyl; GH11) enhanced the dry matter digestibility of maize and wheat in vitro, in comparison with Xyl alone. In broilers, the combination of dietary Xyl and Abf (Rovabio® Advance) enhanced energy, fat, fiber, and protein utilizations. This study shows the effect of feeding low-energy diets with or without Rovabio® Advance, including high concentrations of Xyl and Abf, on the growth performance, nutrient digestibility, lipid peroxidation, blood constituents, and immune response of broilers. Our results confirm the improved growth, digestibility, and immunity obtained by enzymes supplementation. Furthermore, diets supplemented with enzymes caused a higher antibody titer against the Newcastle disease virus. Moreover, they enhanced plasma lipid profiles and antioxidation.The present study was conducted to examine that impact of dietary xylanase (Xyl) and arabinofuranosidase (Abf) supplementation on the performance, protein and fat digestibility, the lipid peroxidation, the plasma biochemical traits, and the immune response of broilers. A total of 480, un-sexed, and one-day-old broilers (Ross 308) were randomly divided into three treatments with eight replicates, where chicks in the first treatment were fed basal diets and served as the control, chicks in the second treatment were fed diets formulated with reductions of 90 kcal/kg, and chicks in the third treatment were fed the same formulated diets used in the second group as well as the Xyl and Abf combination (Rovabio® Advance). Feed intake was decreased by the low energy diet, leading to an enhancement in feed efficiency enzyme supplementation in the low energy diet (p < 0.015). Both protein and fat digestibility were improved (p < 0.047) due to enzyme supplementation. Moreover, enzyme supplementation increased muscle total lipids content and decreased muscle thiobarbituric acid retroactive substance content. Furthermore, diets supplemented with Xyl and Abf exhibited an increase in antibody titers against the Newcastle disease virus (p < 0.026). In addition, enzyme supplementation increased gene expression related to growth and gene expression related to fatty acid synthesis. It could be concluded that dietary Xyl and Abf supplementation had beneficial impacts on growth, nutrient digestibility, lipid peroxidation, immune response, and gene expressions related to growth and fatty acid synthesis in broiler chickens fed low-energy diets.