Glucose Diffusion Rate Research Articles

Preparation, characterisation and in vitro digestibility of potato starch‐fatty acid complexes

SummaryThe starch‐fatty acid (FA) complex belongs to a new type of resistant starch (RS5) and has become a prevalent research topic because of its slow‐digestion and enzymatic resistance properties. In this study, the starch‐FA complexes were prepared from potato starch (PS) and FAs with various carbon chain lengths and unsaturation by hydrothermal treatment (HT), alcohothermal treatment (AT) or ultrasound treatment (UT), and their physicochemical properties and digestibility were interpreted. The PS‐8% lauric acid (Lau) with the highest complex index value (approximately 95%) and RS content (approximately 31%) was obtained by HT; meanwhile, it had a slow glucose diffusion rate and a low free fatty acid release rate in simulated gastrointestinal digestion compared to native PS. Besides, the V‐type crystalline structure was formed obviously after HT, but it was too weak for AT and UT samples to be detected. The gelatinisation viscosity was reduced due to the physical combination of PS and Lau. Therefore, HT was a relatively efficient and safe approach to prepare starch‐FA complex with high RS content, and the obtained starch‐FA complex has the potential to become an alternative functional food ingredient.

Fetomaternal Expression of Glucose Transporters (GLUTs)-Biochemical, Cellular and Clinical Aspects.

Several types of specialized glucose transporters (GLUTs) provide constant glucose transport from the maternal circulation to the developing fetus through the placental barrier from the early stages of pregnancy. GLUT1 is a prominent protein isoform that regulates placental glucose transfer via glucose-facilitated diffusion. The GLUT1 membrane protein density and permeability of the syncytial basal membrane (BM) are the main factors limiting the rate of glucose diffusion in the fetomaternal compartment in physiological conditions. Besides GLUT1, the GLUT3 and GLUT4 isoforms are widely expressed across the human placenta. Numerous medical conditions and molecules, such as hormones, adipokines, and xenobiotics, alter the GLUT’s mRNA and protein expression. Diabetes upregulates the BM GLUT’s density and promotes fetomaternal glucose transport, leading to excessive fetal growth. However, most studies have found no between-group differences in GLUTs’ placental expression in macrosomic and normal control pregnancies. The fetomaternal GLUTs expression may also be influenced by several other conditions, such as chronic hypoxia, preeclampsia, and intrahepatic cholestasis of pregnancy.

Evaluation of the amount of glucose adsorbed on water-soluble dietary fibres by the analysis of its diffusion rate through a dialysis membrane

A model for describing the rate of glucose diffusion through a dialysis membrane was developed. The diffusion coefficient of glucose in the dialysis membrane, D, was constant, regardless of the glucose concentration. The data on the diffusion rate of glucose both in the presence and absence of water-soluble dietary fibres were well described by the model, and the amounts of glucose adsorbed on different fibres were evaluated. The amount of adsorbed glucose increased linearly with an increase in the glucose concentration. A decrease in diffusion rate was caused by adsorption of glucose on water-soluble dietary fibre but not by viscosity. Since the rate of desorption of glucose from dietary fibres is slow enough, the amount of glucose adsorbed is considered to be kept almost constant during the dialysis experiment.

The effects of removing endogenous proteins, β-glucan and lipids on the surface microstructure, water migration and glucose diffusion in vitro of starch in highland barley flour

The effects of removing non-starch components on the surface microstructure, water migration and glucose diffusion rate of highland barley starch (HBS) were systematically studied to reveal the models that affect the digestibility of HBS. The results of scanning electron micrographs, confocal laser scanning microscopy, polarization cross and particle size distribution showed that there were two particle size ranges in highland barley flour, large (73.99–591.90 μm) and small (<73.99 μm). Partial β-glucan and proteins form a compact and continuous matrix on the surface of HBS granules; lipids can partly be adsorbed on the surface of HBS granules, while other lipids, proteins and β-glucan are scattered around HBS granules. After the removal of non-starch components, the ratio of large particles decreased and the starch granules were more exposed, especially proteins and β-glucan. The results of low-field nuclear magnetic resonance investigation showed that the water migration rate was accelerated after the removal of β-glucan, and partial free water was transformed into weakly bound water, while the opposite effect was observed for the removal of proteins and lipids. The glucose diffusion rate in vitro was unchanged after the removal of lipids, while the removal of β-glucan significantly accelerated the diffusion of glucose in vitro. This study reveals that the effective models of HBS inhibited by non-starch components is the compact matrix mainly formed by proteins and β-glucan on the surface of HBS granules. Additionally, the rate of glucose diffusion across the small intestinal wall could be considered as one of the means of constructing slowly digestible starch.

Structure, physicochemical properties and effects on nutrients digestion of modified soluble dietary fiber extracted from sweet potato residue

Alkaline hydrogen peroxide (AHP) modification increased the yield and the content of soluble dietary fiber extracted from sweet potato residue. As compared to the original sweet potato dietary fiber (O-SPDF), AHP modified SPDF (A-SPDF) had a smaller molecular weight of 69073.59 Da and a lower zeta-potential of −27 mV. Monosaccharide composition analysis revealed that A-SPDF had a higher proportion of pectin polysaccharides with homogalacturonan (HG) and rhamnogalacturonan-Ⅰ (RG-Ⅰ) backbones. Fourier transformed infrared (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) analyses were employed to determine the structural differences between A-SPDF and O-SPDF. Characterization of their rheological properties showed that A-SPDF exhibited higher viscosity than O-SPDF at concentrations of 2%, 4% and 6%, respectively. Additionally, A-SPDF had a more gel-like behavior than O-SPDF in the presence of Ca2+, supporting the better functional properties of A-SPDF as determined by water holding capacities (WHC), oil holding capacities (OHC), and water swelling capacities (WSC). Furthermore, A-SPDF performed significantly better in inhibiting starch hydrolysis and reducing the glucose diffusion rate using an Infogest simulated digestion model. However, SPDFs had no impact on the digestion rate of protein. Our results suggested that A-SPDF has the potential to serve as a novel food additive and functional hydrocolloid to attenuate nutrients digestion related disorders, which forms the scientific basis for the better utilization of sweet potato residue and further develop sweet potato dietary fiber (SPDF) as a functional food and/or additive in the food industry.

In-vitro hypoglycemic activity of lawsonia inermis Linn. Leaf extracts

in-vitro hypoglycemic activity of lawsonia inermis Linn. Leaf extracts

Integral use of Argentinean Solanum betaceum red fruits as functional food ingredient to prevent metabolic syndrome: effect of in vitro simulated gastroduodenal digestion

The “red chilto” (Solanum betaceum Cav) is a native fruit from the Yungas forest of Argentina. Red chilto is a neglected and underutilized native species (NUS). The objective of this work was to evaluate the potentiality of pulp, seed and skin from “red chilto” as a functional food ingredient to add value to a native resource of Argentine Yungas to promote sustainable integral use of it. The powders have low carbohydrate and sodium content and are a source of vitamin C, phenolic acids (rosmarinic acid and caffeoylquinic acid), anthocyanins, condensed tannins, carotenoids, potassium, and fiber. The phenolics of chilto powders showed, before and after simulated gastroduodenal digestion, antioxidant activity (ABTS•+; H2O2; O2•; HO•) and were able to inhibit enzymes related to metabolic syndrome, such as α-glucosidase, α-amylase and lipase. Chilto powder showed hypoglycemic effect by increasing glucose adsorption, decreasing glucose diffusion rate and by promoting glucose transport across the cell membrane. These results suggest the potential of Argentinean “red chilto” fruits as functional food ingredients or dietary supplements to prevent metabolic syndrome principally by its antioxidant, hypoglycemic and hypolipemic effects.

Comparative in vitro hypoglycemic studies of unripe, ripe and overripe fruit extract of Musa paradisiaca (Indian banana)

PurposeIn the Indian system of medicine – Ayurveda, Musa paradisiaca has been mentioned as a remedy for various diseases and ailments. Based on the folkloric use, the purpose of this paper is to verify and compare the hypoglycemic potential of unripe, ripe and overripe fruit extract of Musa paradisiaca.Design/methodology/approachHypoglycemic activity of fruit extracts has been evaluated using various in vitro methods, namely, determination of glucose adsorption capacity, glucose uptake in yeast cells, amylolysis kinetics and glucose diffusion.FindingsThe extracts of unripe, ripe and overripe fruits of Musa paradisiaca adsorbed glucose, and the adsorption of glucose increased remarkably with an increase in glucose concentration. In the amylolysis kinetic experimental model, the rate of glucose diffusion was found to increase with time, and all the extracts of unripe, ripe and overripe fruits of Musa paradisiaca demonstrated significant inhibitory effects on the movement of glucose into external solution across the dialysis membrane as compared to the control. The extracts under study also promoted glucose uptake by the yeast cells in all the five glucose concentrations used in the study.Practical implicationsHere, the authors have verified and compared the hypoglycemic potential of Musa paradisiaca, its unripe fruit extract was found to show a better activity than ripe and overripe fruit extracts.Originality/valueBanana, being an all season readily available fruit, is widely consumed due to its ready availability and low cost. It acts as a complete food for even low socio-economic classes of society, owing to its rich nutritional values. Even in a processed and unprocessed manner, it is an important constituent of diet. The research suggests that instead of consuming ripe and overripe fruit, the unripe fruit will help in management of diabetes.

In vitro effects of four polysaccharides containing β-D-Glup on intestinal function

ABSTRACTPlant polysaccharides exert a wide range of biological effects on the intestinal function. In this study, four polysaccharides containing β-D-Glup, including Dendrobium officinale polysaccharide (DOP), Dendrobium aphyllum polysaccharide (DAP), and glucans from yeast (G-Y) and barley (G-B), were investigated in vitro for their intestinal-promoting functions such as inhibition of digestive enzymes, effect of blood glucose and blood lipid levels by using ultraviolet spectrophotometry, as well as the relationship of their structures to those functions. The results indicated that all polysaccharides significantly slowed glucose diffusion rate, decreased protease, and pancreatic lipase activities. Increased branching degrees had a deleterious effect on the α-amylase activity but increased bile acid binding. Increased calcium concentration in glucans (G-Y and G-B) and increased mannose molar ratio in Dendrobium polysaccharides (DOP and DAP) were correlated with increased pancreatic lipase inhibition. The polysaccharides with higher glucose content and branching degrees had higher bile acid binding and disruption of cholesterol micelle formation. These polysaccharides exerted a variety of potential health-promoting effects attributed to their different structures, which should be further investigated in vivo.

Cribado de la actividad hipoglucémica in vitro de Murraya koenigii y Catharanthus roseu

Objetivo: El estudio tuvo como objetivo verificar el efecto hipoglucémico de Murraya koenigii (M. koenigii) y Catharanthus roseus (C. roseus) mediante el uso de diversas técnicas in vitro. Método: Los extractos se estudiaron por sus efectos sobre la capacidad de adsorción de glucosa, la difusión de glucosa in vitro, la cinética de amilolisis in vitro y el transporte de glucosa a través de las células de levadura. Resultados: se observó que los extractos de M. koenigii y C. roseus adsorbieron glucosa y la adsorción de glucosa aumentó notablemente con un aumento en la concentración de glucosa. No hubo diferencias significativas (p≤0.05) entre sus capacidades de adsorción. En el modelo experimental cinético de amilolisis, se encontró que la velocidad de difusión de glucosa aumentaba con el tiempo de 30 a 180 min y ambos extractos de planta exhibían efectos inhibitorios significativos sobre el movimiento de la glucosa hacia la solución externa a través de la membrana de diálisis en comparación con el control. Los extractos también promovieron la absorción de glucosa por las células de levadura y la mejora de la captación de glucosa dependió tanto de la muestra como de la concentración de glucosa. El extracto de M. koenigii exhibió una actividad significativamente mayor (p≤0.05) que el extracto de C. roseus en todas las concentraciones utilizadas en el estudio. Nuestro informe sugiere el mecanismo (s) para el efecto hipoglucemiante de M. koenigii y C. roseus. Conclusión: Se observó que dicho efecto estaba mediado por la inhibición de la alfa amilasa, la inhibición de la difusión de glucosa por la adsorción de glucosa y el aumento del transporte de glucosa a través de las membranas celulares según lo revelado por el modelo in vitro de células de levadura. Sin embargo, estos efectos deben ser afirmados mediante el uso de diferentes modelos in vivo y ensayos clínicos.

EVALUATION OF IN VITRO ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF SEAGRASS HALOPHILA BECCARII

Objectives: The objectives of this study were to analyze the in vitro antidiabetic activity of methanolic extract of seagrass.Methods: The methanolic extract of Halophila beccarii was tested for its in vitro antidiabetic potential through inhibition of carbohydrate digestive enzymes such as α- amylase and α-glucosidase, glucose diffusion assay, glucose adsorption capacity, and enhancement of glucose uptake activity in yeast cells.Results: The crude extract of H. beccarii showed strong inhibition of ɑ-amylase and ɑ-glucosidase as on comparison with the standard enzyme inhibitor acarbose. The antidiabetic activity was found to be concentration dependent, and the antihyperglycemic activity was demonstrated through the inhibition of the glucose movement and facilitated diffusion of glucose. The rate of glucose diffusion across the membrane was found to be increased from 30 to 180 min.Conclusion: The seagrass collected from Pulicat Lake was identified as H. beccarii based on the morphology of the plant, shape of the leaves, and brown pigmentation on the leaves. According to the results, H. beccarii showed antidiabetic activity by the inhibition of carbohydrate digestive enzymes and glucose movement; hence, it is significantly reduced the post-postprandial hyperglycemia.

Physical properties and antidiabetic potential of a novel galactomannan from seeds of Gleditsia japonica var. delavayi

In this paper, the physical properties of a soluble non-starch polysaccharide (EGSP) from the endosperm of Gleditsia japonica var. delavayi seeds, and its effects on starch digestibility, glucose diffusion and glycemic index (GI) were investigated. EGSP has good acid-base and thermal stability and can form high viscosity solutions at low concentrations. Its real density (RD), solubility and lowest-gelled-concentration (LGC) are 1.33 ± 0.03 g/mL, 1.24 ± 0.15 g/100 mL and 20 g/L. Besides, EGSP also had certain oil and glucose adsorption properties. In vitro experiments showed that EGSP could effectively reduce the starch digestibility and glucose diffusion rate, which might be due to its high viscosity and the abilities of hindering starch gelation. Further in vivo experiments confirmed that EGSP can reduce the GI of starchy foods and inhibit the surge of postprandial blood glucose level.

In vitro hypoglycemic effects of unripe and ripe fruits of Musa sapientum

The present study was undertaken to verify the hypoglycemic potential of unripe and ripe fruit extracts of Musa sapientum by using various in-vitro techniques, namely glucose adsorption capacity, glucose diffusion, amylolysis kinetics and glucose transport across the yeast cells. The results revealed that the unripe and ripe fruit extracts of Musa sapientum adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. In the amylolysis kinetic experimental model the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both extracts exhibited significant inhibitory effects on the movement of glucose into external solution across the dialysis membrane as compared to control. The plant extracts also promoted glucose uptake by the yeast cells and enhancement of glucose uptake was dependent on both the sample and glucose concentration. The hypoglycemic effect exhibited by the extracts was observed to be mediated by inhibiting α-amylase, inhibiting glucose diffusion by adsorbing glucose and by increasing glucose transport across the cell membranes as revealed by an in-vitro model of yeast cells.

IN VITRO HYPOGLYCEMIC EFFECTS OF CAESALPINIA BONDUCELLA AND MYRISTICA FRAGRANS SEED EXTRACTS

The present investigation was undertaken to assess the hypoglycemic potential of Caesalpinia bonducella (C.bonducella) and Myristica fragrans (M.fragrans), employing various in vitro techniques. The extracts of seeds of C. bonducella and M. fragrans were studied for their effects on glucose adsorption capacity, in vitro glucose diffusion, in vitro amylolysis kinetics and glucose transport across the yeast cells. It was observed that the plant extracts under study adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. There were no significant (p≤0.05) differences between their adsorption capacities. The results of amylolysis kinetic experimental model revealed that the rate of glucose diffusion was found to be increased with time from 30 to 180 min and both the plant extracts demonstrated significant inhibitory effects on movement of glucose into external solution across dialysis membrane as compared to control. Also, the plant extracts promoted glucose uptake by the yeast cells. It was observed that the enhancement of glucose uptake was dependent on both the sample and glucose concentration. C. bonducella extract exhibited significantly higher (p≤0.05) activity than the extract of M. fragrans at all concentrations. The results of the study verified the hypoglycemic activity of the extracts of C. bonducella and M. fragrans. However, the observed effects exhibited by the extracts of seeds of C. bonducella and M. fragrans need to be confirmed by using different in vivo models and clinical trials for their effective utilization as therapeutic agents in better management of diabetes mellitus.

Improvement of physico-chemical properties and phenolic compounds bioavailability by concentrating dietary fiber of peach (Prunus persica) juice by-product.

This study aimed to concentrate dietary fiber (DF) from peach (Prunus persica) juice by-product (PJBP), to improve its functional properties, and its polyphenols bioavailability. The dietary fiber concentrates (DFCs) were obtained from PJBP using water/ethanol treatments (100:0, 20:80, 50:50, 80:20, and 0:100, v/v) at 1:5 ratio (wet weight/solvent, w/v) for 5 and 20 min at 21 °C. All treatments concentrated condensed tannins, total and insoluble DF, with the highest content found with 100% H2 O treatment. The major polyphenols of DFC were 4-O-caffeoylquinic, chlorogenic, and 1,5-di-O-caffeoylquinic acids. Water and oil retention capacity and maximum glucose diffusion rate were improved mainly with 100% H2 O treatment. Healthy rats were fed with a standard diet supplemented with 8% of PJBP, DFC obtained with 100% H2 O for 5 min, or DFC obtained with 20% EtOH for 5 min. Gastrointestinal digesta weight and viscosity were increased in animals supplemented with 100% H2 O DFC. Moreover, the urinary excretion of polyphenol metabolites, mainly glucuronide and sulfate conjugates, was increased with this treatment, indicating a greater bioavailability of PJBP polyphenols, which was associated with an increased dietary fiber porosity. Water treatment could be used to potentiate PJBP functional properties and polyphenols bioavailability. © 2017 Society of Chemical Industry.

Studies on in-vitro hypoglycemic effects of root bark of Caesalpinia bonducella

The present investigation was undertaken to assess the hypoglycemic potential of ethanolic (ECB) and aqueous extracts (ACB) of root bark of Caesalpinia bonducella using various in vitro techniques. The ethanolic and aqueous extracts of root bark of C.bonducella were studied for their effects on glucose adsorption capacity, in vitro glucose diffusion, in-vitro amylolysis kinetics and glucose transport across membrane of yeast cells. It was observed that the extracts under study adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. The results of amylolysis kinetic experimental model revealed that the rate of glucose diffusion was found to be increased with time from 30 to 180min and both the extracts demonstrated significant inhibitory effects on movement of glucose into external solution across dialysis membrane as compared to control. Also, the extracts under study promoted glucose uptake by the yeast cells. It was observed that the enhancement of glucose uptake was dependent on both the sample and glucose concentration. Ethanolic extract of C.bonducella extract exhibited significantly higher (P≤0.05) activity than the aqueous extract at all concentrations. The results of the study verified the hypoglycemic activity of the extracts of root bark of C.bonducella. However, the observed effects need to be confirmed by using different in vivo models and clinical trials for its effective utilization as a therapeutic agent in effective management of diabetes mellitus.

Hypoglycemic effects of Berberis aristata and Tamarindus indica extracts in vitro

ObjectiveThe objective of the present investigation was to evaluate the hypoglycemic potential of Berberis aristata and Tamarindus indica using various in vitro techniques. MethodsThe selected plant extracts were studied for their effects on glucose adsorption capacity, in vitro glucose diffusion, in vitro amylolysis kinetics and glucose transport across the yeast cells. ResultsIt was observed that both the plant extracts adsorbed glucose and the adsorption of glucose increased remarkably with an increase in glucose concentration. No significant (p⩽0.05) differences were observed between the adsorption capacities of B. aristata and T. indica. The results of amylolysis kinetic experimental model exhibited that the rate of glucose diffusion was increased with time from 30 to 180min and both the plant extracts demonstrated significant inhibitory effects on movement of glucose into external solution across dialysis membrane as compared to control. It was observed that the plant extracts also promoted glucose uptake by the yeast cells. Enhancement of glucose uptake was dependent on both the sample and glucose concentration. B. aristata extract exhibited significantly higher (p⩽0.05) activity than the extract of T. indica at all concentrations. ConclusionThe results of the study verified the hypoglycemic activity of the extracts of B. aristata and T. indica. However, the observed effects need to be confirmed using different in vivo models and clinical trials for their effective utilization as therapeutic agents.

Rice (Oryza sativa japonica) Albumin Suppresses the Elevation of Blood Glucose and Plasma Insulin Levels after Oral Glucose Loading.

The suppressive effect of rice albumin (RA) of 16 kDa on elevation of blood glucose level after oral loading of starch or glucose and its possible mechanism were examined. RA suppressed the increase in blood glucose levels in both the oral starch tolerance test and the oral glucose tolerance test. The blood glucose concentrations 15 min after the oral administration of starch were 144 ± 6 mg/dL for control group and 127 ± 4 mg/dL for RA 200 mg/kg BW group, while those after the oral administration of glucose were 157 ± 7 mg/dL for control group and 137 ± 4 mg/dL for RA 200 mg/kg BW group. However, in the intraperitoneal glucose tolerance test, no significant differences in blood glucose level were observed between RA and the control groups, indicating that RA suppresses the glucose absorption from the small intestine. However, RA did not inhibit the activity of mammalian α-amylase. RA was hydrolyzed to an indigestible high-molecular-weight peptide (HMP) of 14 kDa and low-molecular-weight peptides by pepsin and pancreatin. Furthermore, RA suppressed the glucose diffusion rate through a semipermeable membrane like dietary fibers in vitro. Therefore, the indigestible HMP may adsorb glucose and suppress its absorption from the small intestine.

In vitro physicochemical, phytochemical and functional properties of fiber rich fractions derived from by-products of six fruits.

A comparative study was done on the health promoting and functional properties of the fibers obtained as by-products from six fruits viz., pomace of carambola (Averrhoa carambola L.) and pineapple (Ananas comosus L. Merr), peels of watermelon (Citrullus lanatus), Burmese grape (Baccurea sapida Muell. Arg) and Khasi mandarin orange (Citrus reticulata Blanco), and blossom of seeded banana (Musa balbisiana, ABB). Highest yield of fiber was obtained from Burmese grape peel (BGPL, 79.94±0.41g/100g) and seeded banana blossom (BB 77.18±0.20g/100g). The total dietary fiber content (TDF) was highest in fiber fraction derived from pineapple pomace (PNPM, 79.76±0.42g/100g) and BGPL (67.27±0.39g/100g). All the samples contained insoluble dietary fiber as the major fiber fraction. The fiber samples showed good water holding, oil holding and swelling capacities. The fiber samples exhibited antioxidant activity. All the samples showed good results for glucose adsorption, amylase activity inhibition, glucose diffusion rate and glucose diffusion reduction rate index.

Evaluation of ultrasound and glucose synergy effect on the optical clearing and light penetration for human colon tissue using SD‐OCT

Topical application optical clearing agents (OCAs) can effectively enhance the tissue optical clearing on the human colon tissue, which has been demonstrated in our previous studies. Nevertheless, the strong light scattering still limits the diffusion rate of OCAs and penetration depth of light into the tissue. In this study, in order to further increase the diffusion of the OCA of glucose into tissue, we employ a method to improve the glucose permeability and light penetration with ultrasound (sonophoretic delivery, SP) and glucose (G) synergy on human normal and cancerous colon tissues in vitro, which was measured and quantified with spectral-domain optical coherence tomography (SD-OCT) technology. To evaluate the effect of ultrasound mediation, the percentages of OCT signal enhancement (PSE) and 1/e light-penetration depth were calculated for G alone and ultrasound-G treatments. The PSE was calculated at approximately 313 μm from the sample tissue surface. For normal and cancerous colon tissues the PSE were about 91.1 ± 10.6% and 65.3% ± 12.3% with 30% G/SP, but for the 30% G alone treatment it was about 78.6 ± 11.2% and 54.5% ± 9.3%, respectively. The max value of 1/e light-penetration depth for normal colon tissue was 0.47 ± 0.02 mm with 30% G alone and 0.60 ± 0.05 mm (p < 0.05)with 30% G/SP synergy. However, for the cancerous colon tissue the max value was 0.45 ± 0.01 mm and 0.57 ± 0.03 mm (p < 0.05), respectively. The obtained permeability coefficients showed a significant enhancement with ultrasound mediation. The mean permeability coefficients of 30% G/SP in normal and cancerous colon tissues were (6.3 ± 0.16) × 10(-6) cm/s and (12.1 ± 0.34) × 10(-6) cm/s (p < 0.05), respectively. These preliminary experiments showed that ultrasound can effectively enhance the tissue optical clearing and glucose diffusion rate as well as increase the light-penetration depth into biotissues.