Different Segments Of Small Intestine Research Articles

Effect of nanosilver as feed additive on performance, blood biochemistry, caecal microbiology and intestinal histomorphology of Vanaraja chicken

A study was conducted to evaluate the effect of nanosilver (NS) as feed additive on performance, blood biochemistry and intestinal histomorphology of Vanaraja chicken. Chicks (180 day old) after one week of age were distributed randomly into five treatment groups of four replicates, each having 9 chicks. Treatment groups consisted of T1 (Control) fed basal diet without any additive and in T2, T3, T4 and T5, NS was added to the diet @ 20, 40, 60 and 80 mg/kg diet, respectively. Cumulative body weight gain also improved significantly with the inclusion of NS with highest gain in T5. There was no difference in the feed consumption among various groups including control. Feed conversion ratio was better in T5 compared to other groups. There was no difference in blood biochemical parameters among groups except for the protein levels which were highest in NS supplemented groups than control. The total viable and coliform counts decreased significantly in the group wherein NS was fed in the diet compared to the control. Villus height in different segments of small intestine improved significantly with the inclusion of NS in the diet with better values in T5 followed by T4. From the present study, it could be concluded that nanosilver up to 80 mg/kg level was effective in improving the growth performance, caecal microbiology and intestinal histomorphology of vanaraja birds.

Microbial diversity and metabolic function in duodenum, jejunum and ileum of emu (Dromaius novaehollandiae)

Emus (Dromaius novaehollandiae), a large flightless omnivorous ratite, are farmed for their fat and meat. Emu fat can be rendered into oil for therapeutic and cosmetic use. They are capable of gaining a significant portion of its daily energy requirement from the digestion of plant fibre. Despite of its large body size and low metabolic rate, emus have a relatively simple gastroinstetinal (GI) tract with a short mean digesta retention time. However, little is known about the GI microbial diversity of emus. The objective of this study was to characterize the intraluminal intestinal bacterial community in the different segments of small intestine (duodenum, jejunum, and ileum) using pyrotag sequencing and compare that with the ceca. Gut content samples were collected from each of four adult emus (2 males, 2 females; 5–6 years old) that were free ranged but supplemented with a barley-alfalfa-canola based diet. We amplified the V3-V5 region of 16S rRNA gene to identify the bacterial community using Roche 454 Junior system. After quality trimming, a total of 165,585 sequence reads were obtained from different segments of the small intestine (SI). A total of 701 operational taxonomic units (OTUs) were identified in the different segments of small intestine. Firmicutes (14–99%) and Proteobacteria (0.5–76%) were the most predominant bacterial phyla in the small intestine. Based on species richness estimation (Chao1 index), the average number of estimated OTUs in the small intestinal compartments were 148 in Duodenum, 167 in Jejunum, and 85 in Ileum, respectively. Low number of core OTUs identified in each compartment of small intestine across individual birds (Duodenum: 13 OTUs, Jejunum: 2 OTUs, Ileum: 14 OTUs) indicated unique bacterial community in each bird. Moreover, only 2 OTUs (Escherichia and Sinobacteraceae) were identified as core bacteria along the whole small intestine. PICRUSt analysis has indicated that the detoxification of plant material and environmental chemicals seem to be performed by SI microbiota, especially those in the jejunum. The emu cecal microbiome has more genes than SI segments involving in protective or immune response to enteric pathogens. Microbial digestion and fermentation is mostly in the jejunum and ceca. This is the first study to characterize the microbiota of different compartments of the emu intestines via gut samples and not fecal samples. Results from this study allow us to further investigate the influence of the seasonal and physiological changes of intestinal microbiota on the nutrition of emus and indirectly influence the fatty acid composition of emu fat.

Stress Levels, Mortality, Intestinal Morphometry and Histomorphology of Chabro Broiler Birds Subjected to Varying Degrees of Post Hatch Delay in Feeding.

Kashmir is a part of Jammu and Kashmir State of India where a large chunk of day old chicks are procured from outside the state and these chicks are transported across a distance of hundreds of kilometers over a period of several days. The long distance transport without any access to feed not only subjects the birds to early life stress but also affects their gut morphological development. Therefore, a study was conducted to evaluate the effect of delayed feeding on stress levels, mortality, intestinal morphometry and histomorphology of chabro broiler chicken. A total of 400 day old chabro chicks were randomly divided into 5 groups, each group comprising of four replicates of 20 birds. Chicks allotted to group-1 (G 1) were offered feed at hatchery itself whereas feeding in groups G 2, G 3, G 4 and G 5 were initiated at the farm after the delay of 12, 24, 48 and 72 h, respectively. The results revealed that the heterophil count showed a steady increase from G 1-G 5 and significantly (p<0.05) higher count was found in G 3, G 4 and G 5 compared to G 1. However, the lymphocyte count showed a steady decrease as delay in feeding increased. H:L ratio followed a regular increase from G 1-G 5 and was significantly (p<0.05) higher in G 3, G 4 and G 5 compared to G 1. An overall mortality of 13% was recorded during the trial and all of it occurred during first 2 weeks. The highest overall mortality of 22.50±3.23% was recorded in group G 5 followed by 17.50±6.61% in G 4. The villus height of duodenum and jejunum was significantly (p<0.05) higher in G 2, G 3, G 4 and G 5 as compared to G 1. Crypt depth and muscular is thickness of duodenum was significantly (p>0.05) lower in G 4 and G 5 compared to G 1. The duration of post hatch feeding delay gradually increased the stress level and mortality of birds and also decreased the length of different segments of small intestine in birds. No adverse effect on histomorphology was observed at the end of trial. Feeding at hatchery itself or feeding during transportation of birds would be a viable strategy to overcome the negative effects of delayed feeding in chicken.

Postprandial changes in high density lipoproteins in rats

Objectives: The present study was designed to verify the influence of acute fat loading on high density lipoprotein (HDL) composition, and the involvement of liver and different segments of small intestine in the changes observed.

HERBAL AND PLANT DERIVED NATURAL PRODUCTS AS GROWTH PROMOTING NUTRITIONAL SUPPLEMENTS FOR POULTRY BIRDS: A REVIEW

The p lant derived and he rbal growth promoter s supplemented in the diet or added in the drin king water in the broiler and poultry birds have a promising biological effect on their growth performance, to reduce the pathogenic bacteriological load in different parts of digestive tra ct and to increase villus height in different segments of small intestine mainly in duodenum.

Effect of colibacillosis or coccidiosis on expression of breast cancer resistance protein in small intestine and liver of chickens

Breast cancer resistance protein (BCRP, ABCG2) is a member of ABC (ATP-binding cassette) transporter superfamily that occurs in a variety of tissues including liver and small intestine of animals. As BCRP is involved in drug absorption, distribution, and elimination, modulation of its expression may affect the clinical efficacy of drugs. However, little is known about the effects of coccidiosis or colibacillosis infection on the levels of BCRP expression in chickens. Here, we studied the effect of infection with Escherichia coli (E. coli) or Eimeriida mixture (E. necatrix and E. tenella) on the expression levels of ABCG2 mRNA and BCRP in the different segments of small intestine and liver in chickens. Expression of ABCG2 mRNA or BCRP was detected in the entire small intestine and liver of healthy chickens, and the expression levels in liver and ileum were significantly higher than duodenum and jejunum. Infection with E. coli or Eimeriida mixture resulted in significant decrease in ABCG2 mRNA and BCRP expression in liver, ileum, and jejunum, but not in duodenum, in comparison with noninfection control. The results indicate that coccidiosis or colibacillosis infection inhibits BCRP expression in chickens, which may consequently influence drug distribution and therapeutic efficacy.

Postprandial Changes in High Density Lipoproteins in Rats Subjected to Gavage Administration of Virgin Olive Oil

Background and AimsThe present study was designed to verify the influence of acute fat loading on high density lipoprotein (HDL) composition, and the involvement of liver and different segments of small intestine in the changes observed.Methods and ResultsTo address these issues, rats were administered a bolus of 5-ml of extra-virgin olive oil and sacrificed 4 and 8 hours after feeding. In these animals, lipoproteins were analyzed and gene expressions of apolipoprotein and HDL enzymes were assessed in duodenum, jejunum, ileum and liver. Using this experimental design, total plasma and HDL phospholipids increased at the 8-hour-time-point due to increased sphingomyelin content. An increase in apolipoprotein A4 was also observed mainly in lipid-poor HDL. Increased expression of intestinal Apoa1, Apoa4 and Sgms1 mRNA was accompanied by hepatic decreases in the first two genes in liver. Hepatic expression of Abcg1, Apoa1bp, Apoa2, Apoe, Ptlp, Pon1 and Scarb1 decreased significantly following fat gavage, while no changes were observed for Abca1, Lcat or Pla2g7. Significant associations were also noted for hepatic expression of apolipoproteins and Pon1. Manipulation of postprandial triglycerides using an inhibitor of microsomal transfer protein -CP-346086- or of lipoprotein lipase –tyloxapol- did not influence hepatic expression of Apoa1 or Apoa4 mRNA.ConclusionAll these data indicate that dietary fat modifies the phospholipid composition of rat HDL, suggesting a mechanism of down-regulation of hepatic HDL when intestine is the main source of those particles and a coordinated regulation of hepatic components of these lipoproteins at the mRNA level, independently of plasma postprandial triglycerides.

Phytogenic Growth Promoter as Replacers for Antibiotic Growth Promoter in Poultry Birds

The phytogenic growth promoters supplemented in the diet or added in the drinking water in the broiler birds have a promising biological effect on their growth performance, to reduce the pathogenic bacteriological load in different parts of digestive tract and to increase villus height in different segments of small intestine mainly in duodenum.

Effect of Citric Acid and Microbial Phytase on Small Intestinal Morphology in Broiler Chicken

An experiment was carried out to investigate the effects of citric acid (CA) (0, 3 and 6%) and microbial phytase (MP) (0, 500 and 1000 U/kg) on morphology of different segments of small intestine (duodenum, jejunum and ileum) in broiler chickens fed on corn and soybean meal based diets. The effect of 9 experimental treatments (3×3 factorial design) were assessed using 270 7-d-old Ross 308 male broiler chicks in a randomized complete block design in three replicates of 10 birds each. The mean villi length (VL), crypt depth (CD) and goblet cell number (GCN) in duodenum, jejunum and ileum and villi width (VW) and VL:CD ratio in duodenum was significantly greater for the birds fed on acidified diets compared to the control birds at day 42 of age (P<0.01). Inclusion of 3% CA in diet significantly decreased the epithelial thickness (ET) in duodenum, jejunum and ileum (P<0.01). The birds received diets with 1000 U/kg of MP showed significant increase in CD (P<0.01) and GCN in jejunum (P<0.05), and significant decrease in VL:CD ratio and ET in the duodenum (P<0.01), jejunum (P<0.05) and ileum (P<0.01) segments. No variable of interest were affected by CA × MP interaction. It was concluded that CA and MP independently exhibit positive impact on morphometery of small intestine, toward facilitating the nutrient absorption and reducing the metabolic demands of the intestinal tract in broiler chickens.

Gastrointestinal Absorption of Recombinant Hirudin-2 in Rats

To investigate the absorption of recombinant hirudin-2 (rHV2) after oral administration to rats and its possible absorption mechanism, a series of experiments were carried out. The degradation of (125)I-rHV2 in the luminal contents and variant mucosal subcellular fractions, as well as the effect of degradation inhibition of some adjuvant was investigated. The bioavailability of rHV2, with or without degradation inhibitor after oral administration to rats was estimated, whereas the in situ loop test and everted sac experiment were also conducted to understand more about the gastrointestinal absorption of rHV2 in rats. It was demonstrated that the rHV2 was not stable in the luminal contents and subfraction of the intestinal mucosa. Some enzyme inhibitor, such as bacitracin or casein, could inhibit the degradation to certain degrees. The intact rHV2 molecules were found in the rat plasma after oral administration, and the bioavailability varies obviously, dependent on the analytical method. Some of the enzyme inhibitor could enhance the rHV2 oral absorption. There is no site difference on rHV2 absorption in different segments of small intestine. The possible transport mechanism of rHV2 across the gastrointestinal tract is concerned with the endocytosis process.

Digestion and Absorption of Ingested and Secreted Proteins Labeled with 75Se-Selenomethionine and 35S-Methionine in the Gastrointestinal Tract of the Rat

By labeling exogenous and endogenous proteins with different radioactive amino acids an attempt was made to further establish their fate in the gastro-intestinal tract of the rat. Ovalbumin labeled with 75Se-selenomethionine and 35S-methionine was fed to rats and the rate of disappearance of both amino acids from the stomach and different segments of small intestine was studied. No differences could be observed in the rate of liberation during digestion and in the subsequent absorption of both amino acids. Similarly, a parallel rate of absorption of both methionines was observed from an intestinal segment in situ. Using ovalbumin labeled with 75Se-selenomethionine, the extent of digestion of dietary versus endogenous secreted proteins showed that exogenous proteins emptied from the stomach are rapidly digested and that the small fraction not digested accumulates in the distal jejunum and ileum, together with the majority of the endogenous protein and of the tryptic activity present in the small intestine. In the presence of soybean trypsin inhibitor added to the diet, a marked reduction in the digestion of the exogenous protein resulted, while the endogenous protein content of the intestine remained relatively constant. Labeled endogenous proteins, peptides and amino acids were isolated from the stomach and the small intestine if radioactive methionine was injected intravenously 2 hours before feeding. This indicates that some digestion of endogenous protein was also taking place; however no quantitative estimates could be obtained.