Different Concentrations Of Hg Research Articles

Acute ecotoxicological effects of Hg(CN)2 in Danio rerio (zebrafish).

Artisanal and small-scale gold mining (ASGM) is the largest source of anthropogenic Hg emissions on the planet. In addition, Hg-contaminated tailings are often reprocessed with sodium cyanide (NaCN) to extract the residual gold remaining in the material. This leads to the formation of mercury cyanide (Hg(CN)2) complexes, which are often discharged in untreated form into local drainages, leading to large amounts of free cyanide being released. However, data on mercury-cyanide interactions are scarce. In this study, we investigated the impact of cyanide and Hg bioavailability in zebrafish when added as Hg(CN)2. Different concentrations of Hg(CN)2 and NaCN were used, leading to an LC50 of 0.53 mg.L-1 for NaCN and 0.16 mg.L-1 for Hg(CN)2. Analyzing free cyanide concentrations in aquarium water, >40% dissociation was observed for NaCN and about 5% for Hg(CN)2. The accumulation of total Hg (THg) in the brain, gills, muscle and kidney was quantified. All fish exposed to Hg(CN)2 had higher THg levels than their controls and kidney was the tissue with higher Hg(CN)2 accumulation. Histological effects on the kidney and gills of both cyanides in D. rerio tissues were investigated, suggesting renal alterations in fish exposed to Hg(CN)2 and showing hyperplasia in the gills of animals exposed to NaCN and Hg(CN)2. The results alert to the risks of the presence of these complexes in aquatic environments.

Characteristic investigation and photodetection analysis on Zn1−xHgxO nanoflakes for near ultraviolet-visible photodetectors

The optical band gap of ZnO was modified with the incorporation of Hg ions by co-precipitation method and is taken up for the fabrication of near ultraviolet-visible (NUV-Vis) photodetectors (PDs). Detailed characteristic investigations were carried out using X-ray diffractometer (XRD), transmission electron microscope (TEM), UV-Vis spectrometer and X-ray photoelectron spectrometer (XPS) preceding the fabrication of NUV-Vis photodetectors. The anticipated incorporation of Hg ions into the hexagonal wurtzite structure of ZnO was confirmed by XRD and XPS analysis. A red shift in the ZnO band gap of about ∼0.8 eV was observed from optical analysis. Well-appreciated detection of NUV-Vis wavelength was achieved with PDs fabricated using different concentrations of Hg doped ZnO, particularly 15% Hg doping, which showed a maximum responsivity of 64 mA/W and quantum efficiency of 19.86%.

Gut-on-a-chip for exploring the transport mechanism of Hg(II)

Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion (Hg(II)) transport. However, they cannot reliably simulate the human intestinal microenvironment and monitor cellular physiology in situ; thus, the mechanism of Hg(II) transport in the human intestine is still unclear. Here, a gut-on-a-chip integrated with transepithelial electrical resistance (TEER) sensors and electrochemical sensors is proposed for dynamically simulating the formation of the physical intestinal barrier and monitoring the transport and absorption of Hg(II) in situ. The cellular microenvironment was recreated by applying fluid shear stress (0.02 dyne/cm2) and cyclic mechanical strain (1%, 0.15 Hz). Hg(II) absorption and physical damage to cells were simultaneously monitored by electrochemical and TEER sensors when intestinal epithelial cells were exposed to different concentrations of Hg(II) mixed in culture medium. Hg(II) absorption increased by 23.59% when tensile strain increased from 1% to 5%, and the corresponding expression of Piezo1 and DMT1 on the cell surface was upregulated.

Mercury Induced Tissue Damage, Redox Metabolism, Ion Transport, Apoptosis, and Intestinal Microbiota Change in Red Swamp Crayfish (Procambarus clarkii): Application of Multi-Omics Analysis in Risk Assessment of Hg.

As one of the most toxic elements, mercury (Hg) is a widespread toxicant in aquatic environments. Crayfish are considered suitable for indicating the impact of heavy metals on aquatic crustaceans. Nevertheless, Hg toxicity on Procambarus clarkii is largely unknown. In this research, the acute Hg-induced alterations of biochemical responses, histopathology, hepatopancreatic transcriptome, and intestinal microbiome of Procambarus clarkii were studied. Firstly, Hg induced significant changes in reactive oxygen species (ROS) and malonaldehyde (MDA) content as well as antioxidant enzyme activity. Secondly, Hg exposure caused structural damage to the hepatopancreas (e.g., vacuolization of the epithelium and dilatation of the lumen) as well as to the intestines (e.g., dysregulation of lamina epithelialises and extension of lamina proprias). Thirdly, after treatment with three different concentrations of Hg, RNA-seq assays of the hepatopancreas revealed a large number of differentially expressed genes (DEGs) linked to a specific function. Among the DEGs, a lot of redox metabolism- (e.g., ACOX3, SMOX, GPX3, GLO1, and P4HA1), ion transport- (e.g., MICU3, MCTP, PYX, STEAP3, and SLC30A2), drug metabolism- (e.g., HSP70, HSP90A, CYP2L1, and CYP9E2), immune response- (e.g., SMAD4, HDAC1, and DUOX), and apoptosis-related genes (e.g., CTSL, CASP7, and BIRC2) were identified, which suggests that Hg exposure may perturb the redox equilibrium, disrupt the ion homeostasis, weaken immune response and ability, and cause apoptosis. Fourthly, bacterial 16S rRNA gene sequencing showed that Hg exposure decreased bacterial diversity and dysregulated intestinal microbiome composition. At the phylum level, there was a marked decrease in Proteobacteria and an increase in Firmicutes after exposure to high levels of Hg. With regards to genus, abundances of Bacteroides, Dysgonomonas, and Arcobacter were markedly dysregulated after Hg exposures. Our findings elucidate the mechanisms involved in Hg-mediated toxicity in aquatic crustaceans at the tissue, cellular, molecular as well as microbial levels.

Mercury pollution assessment and metallothionein gene expression in tilapia (Oreochromis mossambicus): a case study of Revuè River in Manica, Mozambique

Revuè River has been considered as one of the most threatened systems in Mozambique due to increased anthropogenic activities such as artisanal and industrial gold mining using mercury (Hg), a heavy metal known for its diverse effects including, bioaccumulation and biomagnification in the environment. The present study aims to determine the metallothionein (MT) expressions in the hepatic tissue of Oreochromis mossambicus (tilapia) exposed to Hg in the laboratory and tilapia collected in Chicamba reservoir. Tilapia juveniles were exposed to different concentrations of Hg (0.037 and 0.37 mg/L) in the laboratory. Water, sediment and fish samples were collected to quantify Total Hg (THg). Furthermore, liver samples in tilapia exposed to Hg and tilapia samples from Chicamba reservoir were collected to analyze the gene expression levels of MT using a quantitative PCR. Results from the study showed a low contamination of the waters and sediment from Revuè River, demonstrated by the Hg concentration in the sediment and water. Slightly high concentrations of Hg were detected in fish from Chicamba reservoir, confirmed by the overexpression of MT observed in liver samples from tilapia and were higher than those expressed in tilapia from the laboratory experiment. There is a correlation between Hg exposure and the MT expression in tilapia, suggesting that MT can be used as a biomarker to assess Hg pollution in the Revuè River.

Conjoint application of novel bacterial isolates on dynamic changes in oxidative stress responses of axenic Brassica juncea L. in Hg-stress soils

This experimental study explores the possible role of three Hg-resistant bacterial strains in the enhanced growth of the mustard plant (Brassica juncea) under Hg-stress conditions. Under different concentrations of Hg, a pot scale experiment with Brassica juncea L. was performed to investigate the potential of bacterial strains for phytoremediation under Hg stress conditions. The results showed that all three strains, as well as their consortium, were capable of stimulating plant growth, biomass, and anti-oxidative enzyme activities. In comparison to the individual strains, the consortiums of all three strains were more prominent in the intensification of Brassica juncea L. physiological activity. Under Hg-stress conditions, all three strains increased the level of antioxidative content in Brassica juncea, indicating an increase in enzyme activity to cope with oxidative stress. Among all the three strains, Citrobacter Freundii (IITISM25) showed the highest accumulation potential in B. juncea followed by Morganella morganii (IITISM23) and Brevundimonas Dimunta (IITISM22). Hence, the results suggest that the IITISM22, IITISM23, IITISM25 strains and their consortium are very effective in phytoremediation and promote Brassica juncea growth under Hg-stress conditions.

Effect of age on the mercury sensitivity of zebrafish (Danio rerio) sperm

The effect of age on the sensitivity of zebrafish sperm against mercury exposure was investigated in the present study. Although results of the use of sperm from mature individuals for toxicity tests have been published, there is no information about the exact age of the fish in some cases, which can affect the results. During the experiments, pooled sperm was stripped from males of 7, 12, or 18 months of age, divided into 5 sub-groups, diluted with different concentrations of Hg (0, 0.5, 1, 2.5, and 5 mg/L Hg), and incubated for 240 min. The motility parameters of sperm (progressive motility (%), curvilinear velocity (VCL)) were measured by a computer-assisted sperm analysis system, at 30, 120, and 240 min of exposure. Regarding the age, significant differences were found in PMOT (p = 0.0267) as well as in VCL (p = 0.0004) among the three different age groups. The different concentrations of Hg also caused significant differences. The most significant differences in PMOT were between the 7- and 18-month-old groups; these differences were observed at 0.5, 1 and 2.5 mg/L Hg at 30 min, at 0.5 and 1 mg/L at 120 min, as well as at 0.5 mg/L at 240 min. In VCL the most significant differences were found between the 7- and 12-month-old groups; significant differences were found at each tested concentration at 30 min as well as at 0.5 and 2.5 mg/L at 240 min. According to the results, the age of zebrafish negatively influences the sensitivity of its sperm. This may concern not only toxicology tests but many techniques in fish breeding where the sperm is treated before use (cryopreservation, pressure shock, etc.).

Transfer and bioaccumulation of mercury from soil in cowpea in gold mining sites

In this study, we evaluated the phytoremediation ability of three different genotypes of cowpea (Vigna unguiculata L. Walp) grown on mercury-contaminated soils from gold mining areas. In particular we compared a native genotype with two commercial lines L-019 and L-042. The plants were cultivated in soils amended at different concentrations of Hg (i.e. 0.2, 1, 2, 5 and 8 mg kg−1). After three months exposure, we determined plant growth, seed production, and Hg accumulation in different plant tissues (root, leaf, seed and stem). Indices of soil-plant metal transfer such as translocation, bioconcentration and bioaccumulation factors were calculated. Results showed that the native variety presented the highest seed production (3.8 g), however the highest plant biomass (7.9 g) was observed in line L-019, both on Hg-contaminated soil of 1 mg kg−1. The different plant tissues differed in terms of Hg concentration (root > leaf > stem). In the highest treated soil, the line L-042 accumulates higher Hg in both roots and leaves, while line L-019 accumulates more metal in stems. In line L-019, Hg concentrations in the fruit showed significant differences being higher in the valves than in the seeds. The transfer factors were generally lower than 1 and indicates the low accumulation of Hg by cowpeas. The estimated daily Hg intake through cowpea consumption showed values far below the threshold of 0.57 μg kg−1 dw day−1 recommended by the World Health Organization. Our results show cowpea V. unguiculata as a good protein-rich food substitute of Hg-contaminated fish for populations living near gold mining sites.

Investigating the Electronic Coefficients in Ne-Hg Mixtures: Comparing a Monte Carlo and Artificial Neural Network Model

Electron coefficients such as drift velocity, ionization coefficient, mean electron energy and Townsend energy for different concentrations of Hg 0.1%, 1%, 10% and 50% in the Ne-Hg mixture at a reduced electric field were calculated using two approaches taking into account inelastic collisions: The Monte Carlo simulation, and an artificial neural network. The effect of Hg vapor concentration on the electron coefficients showed that insignificant additions of mercury atom impurities to Neon, starting from fractions of a percent, affect the characteristics of inelastic processes and discharge, respectively. The aim of this paper is to explore the new applications of neural networks. The Levenberg-Marquardt algorithm and artificial neural network architecture employed was presented in this work to calculate the electron coefficients for different concentrations of Hg in Ne-Hg mixtures. The artificial neural network has been trained with four models (M1, M2, M3, M4), and analysis of the regression between the values of an artificial neural network and Monte Carlo simulation indicates that the M2 output provided the best perfect correlation at 100 Epochs, and the output data obtained was closest to the target data required through using the different stages of artificial neural network development starting with design, training and testing.

Toxicity and Organ Distribution of Mercury in Freshwater Fish (Oreochromis niloticus) after Exposure to Water Contaminated Mercury (HgII).

The purpose of this study was to investigate the toxicity and the distribution of mercury (Hg) in the main tissues of freshwater fish (Oreochromis niloticus) after being exposed to water containing Hg(II). A sample group of 10 fish, of mean weight 80–100 g wet weight, were exposed to different concentrations of Hg (0.0012; 0.0049; 0.0141; 0.0524; 0.1126; and 0.5110 mg-HgII/L) for 72 h under controlled conditions using the static method in ponds. A control medium was also prepared in two replications. Mortality of fish was closely monitored, and the test was repeated three times. For the toxicity test, observations were based on behavior, mortality, and anatomical pathology. The methodology was based on the OECD guidelines for testing of chemicals and lethal concentration (LC50) and particularly using the probit method. Thus, the mean value was obtained from two replications and then further calculated by a software (MiniTab® 16 version). Prior to analysis, samples were first lyophilized. The total concentration of Hg accumulation in the fish organs was analyzed using heat-vaporization atomic absorption spectrometry (HV-AAS) and a MA2000 automatic mercury analyzer. Results showed that toxicity (LC50) of freshwater fish was 0.1435 mg-Hg(II)/L. The internal organs showed some pathological changes including pale gills, anemic eyes, and a whitish body color after the exposure. Furthermore, histopathologically, exposure to mercury might also affect other organs, such as gills, liver, and hepatopancreas. Mercury was found in trace amounts, and its accumulation was found to be at least in the gills. Meanwhile, the highest accumulation was found in the muscle tissue with approximately 5.7183 µg/g dry weight. If they are put in order, the mercury accumulation in the tissue organs was varied from the highest to lowest one: Muscle > eye > bone > head > gill. Finally, it can be concluded that the Hg exposure could affect the histopathological condition of the tested fish.

Growth and physiological responses in chicory towards mercury induced in vitro oxidative stress

Plants experience oxidative stress upon exposure to heavy metals that leads to cellular damage. Plants accumulate metal ions that disturb cellular ionic homeostasis. Currently the main interest in metal accumulators lies in the field of phytoremediation when plants are used to “clean up” metal contaminants from the soil. The present study was conducted to evaluate the oxidative stress in chicory plants when subjected to different concentrations of Hg (0, 25, 50 and 75 µM). In order to study oxidative stress and Hg accumulation in chicory various morphological, biochemical and enzymatic parameters at two different stages (23 and 46 days old) were studied. The root and shoot growth, biomass accumulation declined significantly at highest Hg concentration (75 µM) and Hg accumulation was higher in roots than in shoots as indicated by translocation factor < 1. Hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances content increased with increasing concentration of Hg treatments. The change in H2O2 was also revealed by in vivo histochemical detection. The osmolytes and photosynthetic pigment increased significantly up to 50 µMHg, while as decreasing slightly at higher dose (75 µM). The activities of defense enzymes viz. superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase and glutathione S-transferase were found to be positively correlated with Hg-concentration. In conclusion, the chicory plants were found to possess Hg-detoxification capacity which can be recommended for remediating Hg contaminated soils.

Mercury accumulation from food decreases collembolans' growth

In the terrestrial environment, mercury (Hg) contamination can be originated from different inorganic and metal-organic sources, redistributed and transformed in soils. In the present study, the effects of contaminated food with environmentally relevant concentrations of Hg were evaluated in the soil-dwelling invertebrate Folsomia candida. Changes in growth rate and Hg bioaccumulation levels were observed at different concentrations of Hg in food, which can be complementary for data already available for reproduction and survival from standardized protocols. Collembolan growth was recorded every two days, and their growth rate along with a Von Bertalanffy's growth curve were derived showing that growth was dependent on Hg food concentration. Also, the final length of animals reflected the Hg concentration in food, with differences in all treatments comparing to non-exposed organisms. Toxicokinetic patterns from different Hg concentrations in food were not significantly different during the uptake phase, but differences were found in the depuration phase. Combining the two approaches, collembolans seem to invest their energy for depuration processes, neglecting other vital processes, such as growth. Also, contaminated food avoidance possibly occurred, thus decreasing their feeding and contaminant intake. Therefore, growth tests in collembolans can act as complementary tools to bioaccumulation and reproductive assays, towards a mechanistic understanding of how organisms use their energy upon contamination. Changes in growth rate, even at low and environmentally relevant concentrations, could be a warning signal when occurring in species with key roles in ecosystems. Also, this study highlights the importance of these complementary tests for a better and complete approach to risk assessment studies.

Spatial patterns of mercury contamination and associated risk to piscivorous wading birds of the south central United States.

Piscivorous birds are top predators in aquatic ecosystems and are vulnerable to mercury (Hg) exposure and associated adverse health effects. In some areas of North America, the health risk posed to piscivorous birds by Hg contamination has not been characterized because concentrations of Hg in bird tissues have not been extensively monitored. When data on Hg in tissues of piscivorous birds are not available, the concentration of Hg in the blood of piscivorous birds can be estimated from the concentration of Hg in prey fish. We used concentrations of Hg in different lengths of a proxy prey fish, bluegill (Lepomis macrochirus), to estimate the concentration of Hg in the blood of 4 species of adult piscivorous wading birds (little blue herons [Egretta caerulea], green herons [Butorides virescens], great egrets [Ardea albus], and great blue herons [Ardea herodias]) in 14 ecoregions of the south central United States. The 4 species of birds consume different sizes of fish with different concentrations of Hg and were predicted to have different concentrations of Hg in their blood, with little blue herons < green herons < great egrets < great blue herons. For each species of bird, there were significant differences in average estimated concentrations of Hg in blood between ecoregions, with estimated concentrations of Hg in blood increasing with Hg deposition. The level of predicted risk varied with ecoregion and bird species and was highest for great blue herons. We recommend that future studies of Hg contamination of piscivorous wading birds of the southern United States focus on great blue herons in water bodies within ecoregions that have high Hg deposition. Environ Toxicol Chem 2019;38:160-166. © 2018 SETAC.

Assessing the effect of mercury pollution on cultured benthic foraminifera community using morphological and eDNA metabarcoding approaches

Mercury (Hg) is a highly toxic element for living organisms and is known to bioaccumulate and biomagnify. Here, we analyze the response of benthic foraminifera communities cultured in mesocosm and exposed to different concentrations of Hg. Standard morphological analyses and environmental DNA metabarcoding show evidence that Hg pollution has detrimental effects on benthic foraminifera. The molecular analysis provides a more complete view of foraminiferal communities including the soft-walled single-chambered monothalamiids and small-sized hard-shelled rotaliids and textulariids than the morphological one. Among these taxa that are typically overlooked in morphological studies we found potential bioindicators of Hg pollution. The mesocosm approach proves to be an effective method to study benthic foraminiferal responses to various types and concentrations of pollutants over time. This study further supports foraminiferal metabarcoding as a complementary and/or alternative method to standard biomonitoring program based on the morphological identification of species communities.

Molecular diagnosis of sickle cell anaemia based on SNPs in β-globin gene

This experimental study explores the possible role of three Hg-resistant bacterial strains in the enhanced growth of the mustard plant (Brassica juncea) under Hg-stress conditions. Under different concentrations of Hg, a pot scale experiment with Brassica juncea L. was performed to investigate the potential of bacterial strains for phytoremediation under Hg stress conditions. The results showed that all three strains, as well as their consortium, were capable of stimulating plant growth, biomass, and anti-oxidative enzyme activities. In comparison to the individual strains, the consortiums of all three strains were more prominent in the intensification of Brassica juncea L. physiological activity. Under Hg-stress conditions, all three strains increased the level of antioxidative content in Brassica juncea, indicating an increase in enzyme activity to cope with oxidative stress. Among all the three strains, Citrobacter Freundii (IITISM25) showed the highest accumulation potential in B. juncea followed by Morganella morganii (IITISM23) and Brevundimonas Dimunta (IITISM22). Hence, the results suggest that the IITISM22, IITISM23, IITISM25 strains and their consortium are very effective in phytoremediation and promote Brassica juncea growth under Hg-stress conditions.

Burkholderia tropica as a Potential Microalgal Growth-Promoting Bacterium in the Biosorption of Mercury from Aqueous Solutions

The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the potential of Burkholderia tropica co-immobilized with Chlorella sp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0.041, 1.0, and 10 mg/l) and their exposure to different contact times corresponding to intervals of 1, 2, 4, 8, 16, and 32 h. As expected, microalgal bacterial biomass adhered and grew to form a biofilm on the support. The biosorption data followed pseudo-second-order kinetics, and the adsorption equilibrium was well described by either Langmuir or Freundlich adsorption isotherm, reaching equilibrium from 1 h. In both bacterial and microalgal immobilization systems in the coimmobilization of Chlorella sp. and B. tropica to different concentrations of Hg(II), the kinetics of biosorption of Hg(II) was significantly higher before 60 min of contact time. The highest percentage of biosorption of Hg(II) achieved in the co-immobilization system was 95% at pH 6.4, at 3.6 g of biosorbent, 30 ± 1°C, and a mercury concentration of 1 mg/l before 60 min of contact time. This study showed that co-immobilization with B. tropica has synergistic effects on biosorption of Hg(II) ions and merits consideration in the design of future strategies for the removal of toxic metals.

Effect of Sediments on Bioaccumulation of Mercury in Fish Body in the Water-Level-Fluctuating Zone of the Three Gorges Reservoir Area

Mercury (Hg) in the aquatic environment is easy to accumulate in fish. In order to study the effect of the sediments on Hg accumulation in fish in the water-level-fluctuation zone of the Three Gorges Reservoir, we conducted a 90-days simulated flooding experiment by using the sediments with different concentrations of Hg. Our study showed that the concentrations of the total mercury (THg) and methyl mercury (MeHg) in the overlying water increased after flooding, and the concentrations in the muscle of fish kept increasing in the period of experiment, the concentrations in the viscera and head increased in the earlier period but seemingly decreased in the later period. The bioaccumulated Hg content in the fresh was higher than that in the viscera and head, between which there was no significant difference. Compared with the control group (no sediment), the presence of sediments obviously increased the content of Hg bioaccumulated in fish, and the bioaccumulated Hg level increased with the Hg concentrations in sediment. The THg and MeHg in different fish parts presented a similar variation trend with the BCF ranging 1.93×105-8.89×105 for MeHg and 1.3×103-12.8×103 for inorganic mercury, indicating that MeHg was more prone to accumulate in fish. The MeHg in fish was significantly related with THg, and accumulated MeHg occupied about 80.1% (muscle), 79.3% (visceral) and 66.7% (head) of increased THg. After the reflooding of the sediment in the water-level-fluctuating zone, net methylation could occur with MeHg as the product, and then MeHg would diffuse to overlay water, further increasing the Hg bioaccumulation in fish. Therefore, the potential pollution risk of Hg in the water-level-fluctuationg zone with large area of the Three Gorges Reservoir cannot be ignored.

Tolerance of the forest soil microbiome to increasing mercury concentrations

Mercury (Hg) occurs naturally in soil and can reach high concentrations in the environment due to anthropogenic activities; however, little is known about its impact on the soil microbiome. Here, we tested the impact of different concentrations of Hg on soil bacterial and fungal communities by carrying out microcosm experiments with seven different forest soils. The highest concentration of Hg (32 μg Hg g−1 dry soil) caused severe diversity loss and shifts in the bacterial and fungal community structures and composition. Bioavailable Hg was reduced in soils with the highest proportion of clay, but the impact of Hg on microbial community structures was still evident in these soils. Lower concentrations of Hg (≤3.2 μg Hg g−1 dry soil) had only a limited effect on the soil microbiome. Fungal communities were generally less affected than the bacterial communities. The bacterial Hg-detoxification capacity, as assessed by mercuric reductase gene abundance, was reduced in soils with the lowest amount bioavailable Hg. We found a wide range of Hg-responsive taxa in soils spiked with high amounts of Hg, although they were generally not specific to any soil type or taxonomic group. Overall, our data show that the impacts of Hg on the soil microbiome and its detoxification responses depend on soil characteristics.

Mercury alters the bacterial community structure and diversity in soil even at concentrations lower than the guideline values.

This study evaluated the effect of inorganic mercury (Hg) on bacterial community and diversity in different soils. Three soils-neutral, alkaline and acidic-were spiked with six different concentrations of Hg ranging from 0 to 200mgkg-1 and aged for 90days. At the end of the ageing period, 18 samples from three different soils were investigated for bacterial community structure and soil physicochemical properties. Illumina MiSeq-based 16s ribosomal RNA (rRNA) amplicon sequencing revealed the alteration in the bacterial community between un-spiked control soils and Hg-spiked soils. Among the bacterial groups, Actinobacteria (22.65%) were the most abundant phyla in all samples followed by Proteobacteria (21.95%), Bacteroidetes (4.15%), Firmicutes (2.9%) and Acidobacteria (2.04%). However, the largest group showing increased abundance with higher Hg doses was the unclassified group (45.86%), followed by Proteobacteria. Mercury had a considerable negative impact on key soil functional bacteria such as ammonium oxidizers and nitrifiers. Canonical correspondence analysis (CCA) indicated that among the measured soil properties, Hg had a major influence on bacterial community structure. Furthermore, nonlinear regression analysis confirmed that Hg significantly decreased soil bacterial alpha diversity in lower organic carbon containing neutral and alkaline soils, whereas in acidic soil with higher organic carbon there was no significant correlation. EC20 values obtained by a nonlinear regression analysis indicated that Hg significantly decreased soil bacterial diversity in concentrations lower than several guideline values.

Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

Heavy metals such as mercury (Hg) pose a significant health hazard through bioaccumulation and biomagnification. By penetrating cell membranes, heavy metal ions may lead to pathological conditions. Here we examined the responses of Ammonia parkinsoniana, a benthic foraminiferan, to different concentrations of Hg in the artificial sea water. Confocal images of untreated and treated specimens using fluorescent probes (Nile Red and Acridine Orange) provided an opportunity for visualizing the intracellular lipid accumulation and acidic compartment regulation. With increased Hg over time, we observed an increased number of lipid droplets, which may have acted as a detoxifying organelle where Hg is sequestered and biologically inactivated. Further, Hg seems to promote the proliferation of lysosomes both in terms of number and dimension that, at the highest level of Hg, resulted in cell death. We report, for the first time, the presence of Hg within the foraminiferal cell: at the basal part of pores, in the organic linings of the foramen/septa, and as cytoplasmic accumulations.