Different Concentrations Of Glyphosate Research Articles

The impact of Roundup® Original DI on the hemolymph metabolism and gill and hepatopancreas oxidative balance of Parastacus promatensis (Crustacea, Decapoda, Parastacidae).

In Brazil, glyphosate is present in more than 130 commercial formulations, and its toxic effects have already been tested in different species to understand its impact on biota Decapod crustaceans are widely used as experimental models due to their biology, sensitivity to pollutants, ease of collection, and maintenance under laboratory conditions. We evaluated the changes in metabolism (hemolymph) and oxidative balance markers (gill and hepatopancreas) of a crayfish (Parastacus promatensis) after exposure to Roundup® (active ingredient: glyphosate). The crayfish were captured in the Garapiá stream within the Center for Research and Conservation of Nature Pró-Mata, Brazil. We collected adult animals outside (fall) and during (spring) the breeding season. The animals were transported in buckets with cooled and aerated water from the collection site to the aquatic animal maintenance room at the university. After acclimatization, the animals were exposed to different concentrations of glyphosate (0, 65, 260, 520, and 780 µg/L). The results showed a significant variation in the hemolymph glucose, lactate, and protein levels. We observed variations in the tissue antioxidant enzymatic activity after exposure to glyphosate. Finally, the increase in oxidative damage required a high energy demand from the animals to maintain their fitness, which makes them more vulnerable to stress factors added to the habitat.

Glyphosate- and aminomethylphosphonic acid (AMPA)-induced mortality and residues in juvenile brown trout (Salmo trutta f. fario) exposed at different temperatures

BackgroundGlyphosate is a broad-spectrum, non-selective systemic herbicide with a commonly assumed low potential for accumulation in biota. Nevertheless, glyphosate has been shown to bioaccumulate in the tissues of several organisms. To understand the bioconcentration dynamics of glyphosate in fish, brown trout (Salmo trutta forma fario) of different age were exposed to different concentrations of glyphosate, the formulation Roundup® LB Plus, and the major transformation product aminomethylphosphonic acid (AMPA) for two, three, or four weeks at different temperatures in the laboratory. Mortality rates were determined, and tissue samples were collected at the end of the experiment to ascertain concentrations of glyphosate and AMPA residues by liquid chromatography coupled to mass spectrometry (LC–MS/MS).ResultsBrown trout mortality during exposure to glyphosate or AMPA was considerably higher at 15 °C than at 7 °C. Also, a significant increase in glyphosate concentrations in samples containing muscle, head, backbone, and caudal fin tissue with increasing exposure concentrations and temperatures was observed. Six-month-old fish contained more glyphosate per kg wet weight after exposure than ten-month-old fish. The bioconcentration factors (BCFs) for glyphosate and AMPA were much higher at 15 °C than at 7 °C, but in both cases decreased with higher glyphosate concentrations. The BCF for glyphosate formulated in Roundup® was higher than the one for the parent compound. Approximately 30–42% of the organ-absorbed glyphosate and AMPA remained in the tissues even when the fish were kept in clean water lacking the test substances for three weeks after termination of exposure.ConclusionOur study demonstrated that there is an interaction between glyphosate and ambient temperature in terms of toxicity. Further it was shown that increasing concentrations of glyphosate and AMPA in the surrounding media lead to significantly increased concentrations of these substances in brown trout tissues, although neither bioconcentration nor bioaccumulation of glyphosate in animal tissues is expected due to the high water solubility of this chemical. As a consequence, the uptake of glyphosate by humans through the consumption of contaminated edible fish is very likely.

Mutagenicity, hepatotoxicity, and neurotoxicity of glyphosate and fipronil commercial formulations in Amazon turtles neonates (Podocnemis expansa)

Pesticides are considered one of the main causes of the population decline of reptiles worldwide, with freshwater turtles being particularly susceptible to aquatic contamination. In this context, we investigated the potential mutagenic, hepatotoxic, and neurotoxic effects in neonates of Podocnemis expansa exposed to substrate contaminated with different concentrations of glyphosate and/or fipronil during embryonic development. Eggs collected from the natural environment were artificially incubated in sand moistened with pure water, water added with glyphosate Atar 48® at concentrations of 65 and 6500 μg/L (groups G1 and G2, respectively), water added with fipronil Regent® 800WG at 4 and 400 μg/L (groups F1 and F2, respectively) and, water added with the combination of 65 μg/L glyphosate and 4 μg/L fipronil or with 6500 μg/L glyphosate and 400 μg/L fipronil (groups GF1 and GF2, respectively). For mutagenicity analysis, we evaluated the frequency of micronuclei (MN) and other erythrocyte nuclear abnormalities (ENAs), while for evaluation of hepatotoxicity and neurotoxicity, livers and encephalon were analyzed for histopathological alterations. Exposure to pesticides, alone or in combination, increased the frequency of erythrocyte nuclear abnormalities, particularly blebbed nuclei, moved nuclei, and notched nuclei. Individuals exposed to fipronil exhibited congestion and inflammatory infiltrate in their liver tissue, while, in the encephalon, congestion, and necrosis were present. Our study confirms that the incubation of eggs in substrate polluted with glyphosate and fipronil causes histopathological damage and mutagenic alteration in P. expansa, highlighting the importance of using different biomarkers to evaluate the ecotoxicological effects of these pesticides, especially in oviparous animals.

Lower Concentrations of the Glyphosate-Based Herbicide Roundup® Cause Developmental Defects in Artemia salina.

Roundup® is the most used glyphosate-based herbicide. During agricultural use it may directly contaminate existing aquatic ecosystems, posing severe concerns for the safety of nontarget terrestrial and aquatic organisms. We investigated the outcome of exposure to different concentrations of glyphosate in Roundup on cyst hatchability, toxicity, and teratogenic effects in the aquatic crustacean Artemia salina that inhabits diverse types of salt waters and, as a filter feeder, carries a greater risk of being exposed to pollutants. We found that exposure to 144 and 288 μg/ml glyphosate in Roundup resulted in cysts unable to complete diapause, and hatchability was completely inhibited during all exposure times tested (17-48 h). A glyphosate concentration of 288 μg/ml in Roundup was lethal to A. salina nauplii, and the lower concentrations (9, 18, 36, 72 μg/ml) had no significant effects on viability. In addition, sublethal and environmentally safe concentrations of glyphosate (0.72 μg/ml) in Roundup affected the early development of A. salina nauplii, with significantly decreased body lengths and reduced widths of the tail, abdomen, and head. The increased level of catalase activity observed in nauplii exposed to 0.72 μg/ml glyphosate for 24 h and those exposed to 7.2 and 72 μg/ml glyphosate for 48 h may be linked to excessive reactive oxygen species levels that had been induced by Roundup. In conclusion, Roundup containing >72 μg/ml glyphosate totally inhibited hatching of cysts and exerted toxic effects on A. salina nauplii. The increased prevalence of developmental defects in the nauplii observed at 0.72 μg/ml glyphosate signifies possible teratogenicity of Roundup exposure even at environmentally relevant concentrations of glyphosate, possibly due to disturbance of the antioxidant defenses, which needs further investigation. Environ Toxicol Chem 2023;00:1-9. © 2023 SETAC.

Stacking herbicide detoxification and resistant genes improves glyphosate tolerance and reduces phytotoxicity in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.)

Glyphosate residues retained in the growing meristematic tissues or in grains of glyphosate-resistant crops affect the plants physiological functions and crop yield. Removing glyphosate residues in the plants is desirable with no penalty on crop yield and quality. We report a new combination of scientific strategy to detoxify glyphosate that reduces the residual levels and improve crop resistance. The glyphosate detoxifying enzymes Aldo-keto reductase (AKR1) and mutated glycine oxidase (mGO) with different modes of action were co-expressed with modified EPSPS, which is insensitive to glyphosate in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.). The transgenic tobacco plants expressing individual PsAKR1, mGO, CP4-EPSPS, combinations of PsAKR1:CP4EPSPS, PsAKR1:mGO, and multigene with PsAKR1: mGO: CP4EPSPS genes were developed. The bio-efficacy studies of in-vitro leaf regeneration on different concentrations of glyphosate, seedling bioassay, and spray on transgenic tobacco plants demonstrate that glyphosate detoxification with enhanced resistance. Comparative analysis of the transgenic tobacco plants reveals that double and multigene expressing transgenics had reduced accumulation of shikimic acid, glyphosate, and its primary residue AMPA, and increased levels of sarcosine were observed in all PsAKR1 expressing transgenics. The multigene expressing rice transgenics showed improved glyphosate resistance with yield maintenance. In summary, results suggest that stacking genes with two different detoxification mechanisms and insensitive EPSPS is a potential approach for developing glyphosate-resistant plants with less residual content.

Tolerance of cerrado baru tree (Dipteryx alata) submitted to different doses of glyphosate

Baru tree (Dipteryx alata Vogel.) is a been an alternative to establish an Integration Crop-Livestock-Forestry Systems (ICLFS), combining native species preservation with sustainable food production. However, the management procedure of herbicides for weed control is one of the major limiting factors to include tree components within ICLFS. Thus, the study evaluated the tolerance of D. alata submitted to different concentrations of glyphosate on the plants, to make the introduction of this forest species in ICLFS. The treatments comprised different doses glyphosate: 0.0 (control); 960; 1920; 2880 and 3840 g a.e ha-1. Shoot height, stem diameter, shoot dry weight, root dry weight and total dry weight, as well as Dickson Quality Index and phytotoxicity were assessed. The phytotoxicity in plants varies according to the applied doses of glyphosate, with progressive yellowing in the leaves up to well-developed necrosis and leaf senescence in the highest doses. All doses tested reduced the biometric and qualitative attributes of D. alata seedlings, demonstrating that no treatment was selective, although the plants are tolerant at dose 960 g a.e ha-1 tested. Increases in doses of glyphosate do not cause the death of seedlings D. alata that shows a great potential to compose ICLFS.

Ameliorative potential of vitamin C and E against Roundup-glyphosate induced genotoxicity triggering apoptosis in caprine granulosa cells.

The present study was aimed to investigate the genotoxic and apoptotic effects of glyphosate (GLP) in Roundup formulation along with mitigation of two potent antioxidants that is, vitamin C and E in caprine granulosa cells in vitro. The entire work was done in a dose and time dependent manner where different concentrations of GLP (0.1, 2.0, and 4.0mg/ml) in Roundup and antioxidants (0.5 and 1.0 mM) were employed to culture of granulosa cells for exposure durations of 24, 48, and 72 h. Analysis of GLP-induced geno-toxicity was accomplished by using single cell gel electrophoresis (comet assay) assay. Results have shown increased incidences of DNA fragmentation, evidenced by presence of different types of comets (Type 1-Type 4) in Roundup-GLP- exposed groups in contrast to the control group (Type 0 comet). However, mitigation by both vitamin C and E was significant (p < .05) in combating the GLP-induced genotoxicity in granulosa cells in a concentration- and time-dependent manner. The results of our study provide a clear indication of the ameliorative actions of vitamin C and E against Roundup-GLP-induced genotoxicity that instigate apoptosis in ovarian granulosa cells of caprine.

Genotoxicity of Glyphosate on Cultured Human Lymphocytes.

Glyphosate-based herbicides are the most used herbicides in the world. Despite being widely used, a dispute exists whether glyphosate-based herbicides have a negative effect on human health, particularly genotoxic effects. Therefore, the aim of this study was to investigate glyphosate genotoxicity on cultured human lymphocytes. Cultured human lymphocytes were treated with different concentrations of glyphosate (20, 40, and 200µmol/L). Four toxicity measures were examined: frequency of chromosomal aberrations (CAs), frequency of sister-chromatid exchange (SCE), production of 8-OHdG, and cell kinetics analysis. The results show that glyphosate induced significant (P < 0.05) increases in the levels of SCE at the highest used concentration (200μmol/L). However, no significant elevation in SCE levels was observed at the lower examined concentrations (20 and 40μmol/L). No significant changes in CA were detected at all examined concentrations (P = 0.86). Also, glyphosate did not induce changes to the normal level of 8-OHdG at all examined concentrations (P = 0.98). Last, no significant changes in either mitotic index or proliferative index were observed at any examined concentrations (P > 0.05). The results collectively indicate a lack of genotoxicity and cytotoxicity of glyphosate in cultured human lymphocytes when dealing with environmentally relevant concentrations (20 and 40μmol/L). However, being exposed to higher concentrations (200μmol/L) led to slightly higher level of SCE. Therefore, we recommend cautionary measures when dealing with glyphosate-based herbicides for individuals, such as farmers, who may be extensively exposed to high concentrations of these herbicides.

Glyphosate hormesis attenuates water deficit stress in safflower (Carthamus tinctorius L.) by modulating physiological and biochemical mediators

Changes in photosynthetic machinery can induce physiological and biochemical damage in plants. Low doses of glyphosate have been shown to exert a positive effect in mitigating the deleterious effects of water deficit in plants. Here, the physiological and biochemical mechanisms of safflower plants (Carthamus tinctorius L.) were studied under conditions of water deficit mediated by the attenuating effect of low-dose glyphosate. The plants were divided into two groups of water regimes in soil, without water deficit (−10 kPa) and with water deficit (−70 kPa), and were exposed to different concentrations of glyphosate (0, 1.8, 3.6, 7.2, 18, 36, 72, 180, 360, and 720 g a.e. ha−1). Evident protective responses at the physiological and biochemical levels were obtained after applying low doses of glyphosate to plants under water deficit, with a limiting dose for the occurrence of hormesis (LDS) = 72 g a.e. ha−1. The water deficit in plants resulted in hydrogen peroxide (H2O2) accumulation and consequently lipid peroxidation (LPO) associated with the accumulation of shikimic acid and glyphosate in plants, which triggered an increase in the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) that act by dismuting the levels of reactive oxygen species (ROS), maintaining, and/or increasing the maximum quantum efficiency of photosystem II (Fv/Fm), effective quantum yield of photosystem II (ΦPSII), electron transport rate (ETR), photochemical extinction coefficient (qP), and non-photochemical extinction coefficient (NPQ). APX appears to be the main enzyme involved in eliminating H2O2. Low doses of glyphosate act as water deficit ameliorators, allowing the plant to maintain/increase metabolism at physiological and biochemical levels by activating antioxidant enzymes in the dismutation of ROS in safflower plants.

Ecotoxicological test to assess effects of herbicides on spore germination of Rhizophagus clarus and Gigaspora albida

Given the essential role of arbuscular mycorrhizal fungi (AMF) in soil systems and agriculture, their use as biological indicators has risen in all fields of microbiology research. However, AMF sensitivity to chemical pesticides is poorly understood in field conditions, and not explored in ecotoxicology protocols. Hence, the goal of this study was to evaluate the effects of different concentrations of glyphosate (Roundup®) and diuron+paraquat (Gramocil®) on the germination of spores of Gigaspora albida and Rhizophagus clarus in a tropical artificial soil. This study was conducted in 2019 at the Soil Ecology and Ecotoxicology Laboratory of the Universidade do Estado de Santa Catarina. The nominal concentrations of glyphosate were 0, 10, 50, 100, 250, 500, 750 and 1000 mg a.i. kg−1. For diuron+paraquat, the concentrations tested were 0, 10 + 20, 50 + 100, 100 + 200, 250 + 500, 500 + 1000, 750 + 1500 and 1000 + 2000 mg a.i. kg−1. Glyphosate did not alter germination of G. albida, but germination inhibition of R. clarus spores was of 30.8% at 1000 mg kg−1. Diuron+paraquat inhibited by 8.0% germination of G. albida, but only at the highest concentration tested. On the other hand, effects on R. clarus were detected at 50 + 100 mg kg−1 concentration and above, and inhibition was as high as 57.7% at the highest concentration evaluated. These results suggest distinct response mechanisms of Rhizophagus and Gigaspora when exposed to herbicides, with the former being more sensitive than the later.

Glyphosate-induced injury in neurons, endothelial cells and myoblasts is mediated by ROS production

Background Glyphosate (N-(phosphonomethyl) glycine) is the active compound of a large part of non-selective herbicides, being the most used in agriculture worldwide since middle 70s. Glyphosate acts as an inhibitor of the shikimate pathway, which is present only in plants and some microorganisms. Despite this specific mechanism of action, the safety of glyphosate in mammals is still controversial. Aim This study aims to elucidate the direct biological effects of glyphosate and AMPA (one of its major metabolites), in three in vitro mammalian cell lines: neurons, myoblasts and endothelial cells. Samples are treated with different concentrations of glyphosate (5, 10 and 20 mM) or AMPA (10 and 20 mM), for 1, 2 or 24 hours. Cells are assessed for viability, ROS production and phenotypical changes. Results Glyphosate differentially affects neurons, endothelial cells and myoblasts, with a higher susceptibility of neurons and a higher tolerance of myoblasts. In all cell types, the response is dose-dependent. In terms of ROS production, results are in accordance to the pattern of viability. Morphology shows signs of cavitation of plasma membranes and intracellular compartments. Similarly, AMPA treatment reproduced the same effects in all cell types, but to a lower extent. Discussion Considering the future exposure to low but constant levels of glyphosate, it is of pivotal importance to elucidate its mechanism of actions in mammalian cells. In light of our results, we hypothesize an involvement of mitochondria in the effects of glyphosate. Different amount of mitochondria and antioxidant enzymes, in the different cell types, will be investigated as a prosecution of the study.

Effects of two rhizobacteria inoculants on maize growth performance at different concentrations of glyphosate

The use of Rhizobacteria as biofertilizer is on the increase due to the ability of some of the bacteria to solubilize some insoluble essential nutrients in the soil and produce phytohormones necessary for plant growth. The effectiveness of two plant growth promoting rhizobacteria (Bacillus cereus and Pseudomonas aeruginosa) in plant growth promotion at different concentrations of glyphosate were evaluated. Some agronomic parameters such as plant height, size of girth, number of leaves on the screen house and field were measured and recorded. The results of the effects of P. aeruginosa on the height of maize at different concentrations showed that the plants inoculated with the isolates and planted on the soil without glyphosate (control) recorded the highest height on the 2nd (34.9 cm), 4th (52.45 cm), 6th (61.17 cm) and 8th (66.25 cm) weeks after planting, when compared to those planted on the soil spiked with different concentrations of glyphosate. The effects of the isolates on the size of girth of maize on the soil spiked with different concentrations of glyphosate showed the highest girth size on the soil inoculated with P. aeruginosa eight weeks after planting (8 WAP) with a girth size of 2.0cm and least at 14.4 mg/ml of glyphosate with a girth size of 1.2 cm at 8 weeks after planting. Similar trend was observed on the soil inoculated with B. cereus (without glyphosate) with the highest girth in the 2nd and 4th WAP (1.02 and 1.42 cm, respectively). The results of our field studies showed no significant difference (P≤0.05) in the height and number of leaves of the maize at different treatments and time (weeks after planting). Similar trend was observed i n the yield of maize. This study has shown that these isolates can be useful as biofertilizers especially in the absence or at low concentration of glyphosate.&#x0D; Keywords: Rhizobacteria, maize, Inoculants, Plant-growth-promotion.

Bacillus aryabhattai FACU: A promising bacterial strain capable of manipulate the glyphosate herbicide residues.

Glyphosate is a commonly used organophosphate herbicide that has an adverse impact on humans, mammals and soil microbial ecosystems. The redundant utilize of glyphosate to control weed growth cause the pollution of the soil environment by this chemical. The discharge of glyphosate in the agricultural drainage can also cause serious environmental damage and water pollution problems. Therefore, it is important to develop methods for enhancing glyphosate degradation in the soil through bioremediation. In this study, thirty bacterial isolates were selected from an agro-industrial zone located in Sadat City of Monufia Governorate, Egypt. The isolates were able to grow in LB medium supplemented with 7.2 mg/ml glyphosate. Ten isolates only had the ability to grow in a medium containing different concentrations of glyphosate (50, 100, 150, 200 and 250 mg/ml). The FACU3 bacterial isolate showed the highest CFU in the different concentrations of glyphosate. The FACU3 isolate was Gram-positive, spore-forming and rod-shape bacteria. Based on API 50 CHB/E medium kit, biochemical properties and 16S rRNA gene sequencing, the FACU3 isolate was identified as Bacillus aryabhattai. Different bioinformatics tools, including multiple sequence alignment (MSA), basic local alignment search tool (BLAST) and primer alignment, were used to design specific primers for goxB gene amplification and isolation. The goxB gene encodes FAD-dependent glyphosate oxidase enzyme that responsible for biodegradation process. The selected primers were successfully used to amplify the goxB gene from Bacillus aryabhattai FACU3. The results indicated that the Bacillus aryabhattai FACU3 can be utilized in glyphosate-contaminated environments for bioremediation. According to our knowledge, this is the first time to isolate of FAD-dependent glyphosate oxidase (goxB) gene from Bacillus aryabhattai.

Palatability of glyphosate in ants: a field experiment reveals broad acceptance of highly polluted solutions in a Mediterranean ant.

Glyphosate is a systemic herbicide still used in many countries, though there are several known detrimental effects on animals. Previous studies concerning its effects on social insects are available, but they are primarily focused on honeybees; little is known about the interactions of this compound with ants. Here, we assessed whether different concentrations of glyphosate can be perceived by ant workers and to what extent. As a model species, we used the Mediterranean ant Crematogaster scutellaris, commonly found in agroecosystems. We performed 3000 individual tests of acceptance using ten different solutions of various concentrations of the herbicide. Half of the solutions contained added sucrose in order to test the possible masking effect of the sugar taste on glyphosate. We used comparable glyphosate concentrations to those previously used in other studies on social insects or suggested by the producer. We found that the acceptance of the solutions decreased as the concentration of the herbicide increased. However, a significant percentage of ants drank the solutions with concentrations up to dozens of times higher than those inducing toxic effects in bees. In light of these results, we urge further assessment of the effects of glyphosate on ants, particularly because the food ingested by workers is transferred to the brood and queens, posing a potential threat to the health of the entire colony. Surprisingly, we did not record any difference in acceptance between solutions with and without sugar; this point is discussed regarding drought stress.

Monocrystalline silicon/polyaniline/horseradish peroxidase enzyme electrode obtained by the electrodeposition method for the electrochemical detection of glyphosate

A silicon/polyaniline/horseradish peroxidase enzyme (n-Si/PANI/HRP) electrode was obtained by potentiostatic electrodeposition processes. PANI thin films were electropolymerized on a silicon substrate at different electric potentials, and an HRP enzyme was electrodeposited on the best PANI thin film. The best PANI thin film was obtained under electrodeposition conditions of + 1.00 V (vs. Ag/AgCl) and 900 s, and the immobilization of the HRP enzyme was performed at an electric potential of − 0.50 V (vs. Ag/AgCl). The n-Si/PANI- and n-Si/PANI/HRP electrodes were characterized using scanning electron microscopy and atomic force microscopy. The detection capacity of the glyphosate of the n-Si/PANI/HRP electrode with the best morphological characteristics was evaluated according to the electric current density responses from the oxidation of hydroquinone molecules in a solution containing different concentrations of glyphosate. The detection was performed because of the inhibition of the HRP enzyme activity by the glyphosate molecules in the solutions with a lower concentration limit of 5.44 μg L−1 over an electric potential range of + 0.65 to + 1.15 V (vs. Ag/AgCl).

Pesticide-induced alteration in proteins of characterized soil microbiota revealed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE)

Beneficial soil bacteria including Pseudomonas fluorescens, Burkholderia, cepacia, Azotobacter vinelandii, Rhizobium leguminosarum and Bradyrhizobium japonicum identified by 16S rRNA gene sequence analysis tolerated maximum concentrations of glyphosate (herbicide) and kitazin (fungicide). Pesticides declined the phytohormone-synthesizing ability of PGPR strains. As an example, the higher (3X) level of kitazin reduced maximally IAA synthesizing ability of PGPR strains PS3, PSBB1, AZ2, RP1 and RV9 by 51, 28, 82, 35 and 59%, respectively, than control. Impact of different concentrations of glyphosate and kitazin on bacterial proteins differed considerably (p < 0.05). For instance, total protein content each of PS3 (111.3 μg mL−1), PSBB1 (80.2 μg mL−1), AZ2 (76.3 μg mL−1), RP1 (94.2 μg mL−1) and RV9 (62 μg mL−1) was maximally decreased by 34, 45, 85, 34 and 80%, respectively, at 3X doses of kitazin. Also, total whole-cell bacterial proteins revealed by SDS-PAGE declined in a concentration-dependent manner. As glyphosate and kitazin concentrations increased, protein bands were either decreased/disappeared or induced the formation of some new bands. For example, 400, 800 and 1200 μgmL−1 of kitazin-treated extract of P. fluorescens PS3 showed some new bands of around 200, 125, 90 and 70 kDa, whereas protein bands of 250, 150, 100, 75 and 50 kDa were vanished/disappeared. This study indicates that both glyphosate and kitazin are inhibitory to phytohormone production and bacterial protein synthesis mechanism and hence, provides baseline data that warrant use of pesticides in soil/rhizosphere where such bacterial communities are used as microbial inoculants.

Influence of Glyphosate in the Fungus Trichoderma Harzianum Coming from Rhizoctonia Solani in Huancayo 2019

The objective of this research is to determine the influence of glyphosate on the conidia count of the Trichoderma harzianum fungus grown under laboratory conditions. This fungus is found in all types of soil, but it is more important in those for agricultural use where they fulfill a beneficial function. Glyphosate is an herbicide widely used in the elimination of weeds and shrubs, its effect can be extended to beneficial fungi and its permanence in the soil exceeds the specifications in the data sheet. For this reason, the culture of T. harzianum is carried out under direct exposure with different concentrations of glyphosate, the average dose (1 ml) in the investigation arises from that recommended by the manufacturer of the product and four more are established, two major doses (1.25 ml and 1.50 ml) and two minor doses (0.50 ml and 0.75 ml). Data was collected using observation tokens. All doses were observed to decrease the number of conidia including the mean 1 ml dose producing a 54% reduction compared to the control sample. The doses of 1.25 ml and 1.50 ml reduce up to 65% and 81% respectively. Therefore, this herbicide negatively influences the growth of the fungus and consequently inhibits its beneficial actions.

Cytotoxic evaluation of glyphosate, using Allium cepa L. as bioindicator

Glyphosate is a chemical compound used mainly as a broad spectrum herbicide, it is recognized for its proven effectiveness and easy handling. It represents more than 60% of the world market of non-selective herbicides and is used in both agricultural fields and family gardens. The present study was designed to test the cytogenotoxic potential of glyphosate using the Allium cepa test as toxicity bioindicator. Consequently, bulbs of A. cepa were exposed to different concentrations of glyphosate (5, 10, 15, 25 and 30 mgL−1) and a control (deionized water), for 72 h; root development was also studied in this period of time. The cytogenotoxic potential of glyphosate was determined by calculating the mitotic index (MI), cellular anomalies (CA) and registering the roots longitudinal growth at 24, 48 and 72 h. Regarding root development, a greater growth was observed in the control treatment in the three measurement times. The mitotic phases analysis, determined that the higher the concentration, the lower the mitotic index, in addition the inhibition of the telophase Mitotic Index (TMI) was observed in any of the concentrations. The results indicate that the exposure to glyphosate of A. cepa meristematic cells induces diverse types of chromosomal anomalies, such as micronuclei (MN), chromosome breaking (CB), nuclear notch (Nn), among others. Therefore, in demonstrates that glyphosate has a highly cytogenotoxic effect for any of the concentrations used.

Lethal Toxicity of Glyphosate Herbicide on Koi Carp, Cyprinus carpio (Linnaeus, 1758) Fingerlings

Bioassay experiments were conducted to determine the lethal toxicity of glyphosate herbicide on Koi carp, Cyprinus carpio fingerlings. Koi carp fingerlings with mean length 8.06 ± 0.99 cm were obtained from a freshwater fish seed hatchery of the university. The fishes were exposed to different concentrations of glyphosate and mortality was recorded after every 6 h for a period of 96 h. The 96 h LC 50 concentration for glyphosate on Koi carp fingerlings was found to be 33.2 mgL -1 . The results indicated that glyphosate had impacts on exposed fish, hence, the need for regulation of usage to protect non-targeted species and the environment.

Acute toxicity of glyphosate on various life stages of calanoid copepod, Pseudodiaptomus annandalei

Copepods are one of the most important primary producers and biodiversity indicators. They are also highly susceptible to various toxicants. In this study, glyphosate (Roundup®), a widely used herbicide was used to investigate the toxicity effect on calanoid copepods, Pseudodiaptomus annandalei, focused on their nauplius, copepodid, and adult stages. Different concentrations of glyphosate (i.e. 0 - as control, 0.05, 0.1, 0.4, 1.6, 6.4 and 25.6 mg/L) were used to elucidate the tolerance level of P. annandalei. The survival rate of copepod was recorded at the intervals of 24, 48, 72 and 96 h after glyphosate exposure. The analysis was performed using probit test to determine the sub-lethal concentrations. Our results revealed that LC50 of the nauplius stage was recorded as 3.47, 3.02, 1.86 and 1.10 mg/L at 24, 48, 72 and 96 h, respectively. Higher LC50 values were recorded at 4.36 mg/L for 24 h, 3.09 mg/L for 48 h, 2.00 mg/L for 72 h, and 1.12 mg/L for 96 h at the copepodid stage. Generally, adult copepods showed a higher level of tolerance to glyphosate among all stages, whereby at this stage LC50 values were recorded as 11.70 mg/L for 24 h,10.23 mg/L for 48 h, 7.41 mg/L for 72 h, and 3.61 mg/L for 96 h, respectively. Our results indicated that prolong exposure time of glyphosate could increase the susceptibility of P. annandalei to the herbicide. Nauplii are the most sensitive group among all. This study showed that glyphosate could post significant eco-toxicological impact to the non-targeted organism.