Different Concentrations Of Calcium Chloride Research Articles

Effect of Calcium Chloride and Gibberellic Acid on the Vegetative Growth of Two Cultivars of Gladiolus Gladiolus hybrid L.

This study was conducted inside the wooden canopy of the Department of Horticulture and Landscape Engineering / College of Agriculture and Forestry / University of Mosul during the agricultural season 2022-2023. To study the effect of spraying with different concentrations of calcium chloride (0, 500, 1000) mg. L-1 and spraying with gibberellic acid at concentrations of (0, 100, 200) mg. L-1 and the interaction between them in vegetative of two cultivars of Gladiolus Gladiolus hybrid. L. They are Nova lux with yellow flowers and Trader Lux with red flowers. The corms were planted on 17/3/2022, then sprayed 30 days after planting the corms with three sprays and three concentrations of calcium chloride (0,500, 1000) mg.L-1 The plants were also sprayed with gibberellic acid, with three sprays and three concentrations (0, 100, 200), The period between one spray and another was two weeks. The spraying was done with gibberellic acid after 35 days of planting the corms. The study was carried out using a factorial experiment in the design of completed randomized sectors R.C.B.D within split plots with three replications, each experimental unit included (7) cormat for each replicate. The research results of were analyzed statistically with the (SAS) program and tested statistically using Duncan’s multinomial test at the probability level of 0.05. It was found that the red cultivar recorded the largest plant height, which reached 129.905 cm, the largest leaf length, which reached 102.584 cm, and the largest number of leaves, which reached 9,980 leaves. Plant-1, and the largest leaf area of the plant, amounting to 401.656 cm2. plant-1, and the largest dry weight of the leaves, amounting to 12.567 g. The yellow variety plants also recorded the largest values for the percentage of total chlorophyll in the plant, which amounted to 0.984%. The treatment with gibberellic acid at a concentration of 200 mg also resulted. L-1 led to a significant increase in all studied traits.

Effect of Calcium Chloride and Gibberellic Acid on Flowering Growth of Two Cultivars of Gladiolus X hortulanus L.

This study was conducted inside the wooden canopy of the Department of Horticulture and Landscape Engineering / College of Agriculture and Forestry / University of Mosul during the agricultural season (2022-2023). to study the effect of spraying with different concentrations of calcium chloride (0, 500, 1000) mg. L-1 and spraying with gibberellic acid at concentrations of (0, 100, 200) mg. L-1 and the interaction between flowering growth of two varieties of Gladiolus hortulanus. They are Nova lux with yellow flowers and Trader Lux with red flowers. The corms were planted on 17/3/2022, then sprayed 30 days after planting the corms with three sprays and three concentrations of calcium chloride (0,500, 1000) mg.L-1 The plants were also sprayed with gibberellic acid, with three sprays and three concentrations (0, 100, 200), The period between one spray and another was two weeks. The spraying was done with gibberellic acid after 35 days of planting the corms. The study was carried out using a factorial experiment in the design of completed randomized sectors R.C.B.D within split plots with three replications, each experimental unit included (7) corms for each replicate. The yellow cultivar recorded the shortest period for color to appear in the first inflorescence, which amounted to 79.092 days, and the largest values in the fresh weight of the flower and the inflorescence, which amounted to 50.663 g. The diameter of the first flower reached 78.794 mm, the largest length of the inflorescence reached 41.285 cm, and the largest diameter of the flower stalk reached 9.294 mm. Also, there were no significant differences between the two cultivars in the characteristic of the number of florets per plant. The treatment with calcium chloride at both concentrations of 500 and 1000 mg.L-1 resulted in a significant increase in all studied traits. The treatment resulted in gibberellic acid at a concentration of 200 mg. L-1 resulted in a significant increase in all studied traits.

Quantitative relationship between soil pH and electrical conductivity values and cadmium phytoavailability for Chinese cabbage under simulated conditions

Pot experiments were conducted to investigate the impacts of continuous addition of different concentrations of calcium chloride (CaCl2) and/or low-molecular-weight organic acids (LMWOAs) on soil pH, electrical conductivity (EC), and cadmium (Cd) transformation. These factors subsequently affected Cd phytoavailability in a system consisting of Cd-contaminated soil and Chinese cabbage (Brassica chinensis L.). The results indicate that CaCl2 addition had a greater impact on reducing soil pH value, increasing soil EC value, and enhancing Cd phytoaccumulation in Chinese cabbage compared to LMWOAs. When soil pH dropped by 0.3 unit and the soil EC increased by 500 µS cm−1, the Cd concentration in the Chinese cabbage shoots was 3 times higher than that in the control group. Throughout two planting terms of Chinese cabbage, the addition of CaCl2 (1.6–3.2 g kg−1) and LMWOAs (≤ 1.0 g kg−1) led to phytoextracted Cd concentration exceeding exchangeable Cd concentration in soil samples before the pot experiment. Regarding phytoextracted Cd, desorption from carbonate-bound Cd contributes more than desorption from bound to organic matter Cd and adsorption to Fe/Mn oxide Cd. This study underscores the influence of soil pH and EC value variations and Cd transformation on Cd phytoavailability. Special attention should be given to leafy vegetables grown in Cd-contaminated soil, as the phytoavailable Cd concentration reaches approximately 2.0 µg kg−1, which may lead to Cd levels surpassing acceptable limits for Chinese cabbage.

Preparation and optimization of colon-targeted Pectin-Chitosan microsphere containing Diloxanide furoate-hydroxypropyl-β-cyclodextrin inclusion complex for effective treatment of amoebiasis

Degradation in the upper gastrointestinal tract increases the unwanted dose of Diloxanide furoate.(DF) as an anti-amoebic drug, the use of colonic delivery of it is a promising approach to reduce its dose and dose-dependent side effects. An investigation was done to prepare and evaluate the microsphere for site-specific delivery using surface response curve methodology to optimize independent variables like concentration of polymer (Pectin and Chitosan), crosslinking agent, and time of hardening and the dependent variable like percentage drug entrapment and drug release in an acidic environment. The microsphere was prepared by dropping of optimized inclusion complex of DF and hydroxypropyl-β-cyclodextrin with anionic polymer (Pectin) to a solution of cationic polymer containing different concentrations of calcium chloride. Prepared microspheres were characterized by swelling behavior, In vitro release in various simulated gastric and intestine fluids. The results suggest that pectin 6%, Chitosan 3.5%, CaCl2 6%, and cross-linking time 20 min shows a promising approach to colonic delivery of DF for not only better retreatment of amoebiasis but also for reducing dose-related side effects.

Effect of Exogenous Calcium on the Heat Tolerance in Rosa hybrida ‘Carolla’

This study was designed to investigate the effects of exogenous calcium on the tolerance of Rosa hybrida ‘Carolla’ to high-temperature and the physiological mechanisms underlying this response. Leaves of ‘Carolla’ grown under stress were treated by spraying four different concentrations of calcium chloride (CaCl2; 50, 100, 150, or 200 μM). The photosynthetic responses, antioxidant enzyme activities, and osmotic substance contents were measured under high-temperature stress at the flowering stage. Temperature-stressed ‘Carolla’ with CaCl2 treatment showed significantly increased chlorophyll (Chl) content, net photosynthetic rate (An), transpiration rate (E), stomatal conductance (gs), water use efficiency (WUE), superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) activities together with proline (Pro), soluble sugar (SS), and soluble protein (SP) concentrations, while malonaldehyde (MDA) content and relative electrical conductivity (REC) were significantly reduced. The damages caused by high-temperature stress were alleviated by applying CaCl2. Among the CaCl2 treatments, 100 μM CaCl2 best minimized the damage caused by high-temperature to ‘Carolla’. This study showed that exogenous calcium could improve the tolerance of Rosa hybrida ‘Carolla’ to high-temperature stress by regulating photosynthesis, the antioxidant system, and osmotic substances.

Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction.

Natural polymer-based hydrogel films possess considerable potential for use in biomedical applications and are excellent for wound healing. The purpose of this research was to use ionic crosslinking to improve the mechanical characteristics, absorption of fluid in the wound, and drug release behavior of Cassia alata L. (CA) extract loaded niosomes (CANs) that were incorporated in an alginate-pectin film (A/P). Then, chemically crosslinked A/P hydrogels were obtained by immersing them in different concentrations of calcium chloride (CaCl2) (0.5–1% w/v) for 15–120 s. The degree of crosslinking was controlled by both contact time and CaCl2 concentration. The optimal crosslinking conditions were 1% CaCl2 for 15 seconds. In this study, the following features of the hydrogel films were investigated: physical properties, morphological characteristics, drug loading, in vitro drug release, antibacterial activity, wound healing activity, cytocompatibility profiles, and hemocompatibility. The crosslinked hydrogel films maintained their physical integrity during use, with the 1% film attaining the best results in the shortest period (15 sec). Then, in vitro drug release from the films was examined. Crosslinking was observed to prolong the release of the CA extract from the hydrogel film. Finally, a cell viability experiment was conducted to evaluate the cytotoxicity profile. The A/P composite film exhibited excellent wound dressing qualities and good mechanical properties in preformulation testing. The in vitro drug release profile indicated that the A/P created a regulated drug release profile, and the cell viability experiment revealed that the film was nontoxic and hemocompatible. A/P composite films can be produced using CAN extract as a possible wound dressing. However, further studies in animals and humans are required to determine both safety and effectiveness.

Effect of calcium chloride on reducing Colletotrichum gloeosporioides (Penz.) Sacc. infection on post-harvest fruits of Carica papaya L. ​

Anthracnose (Colletotrichum gloeosporioides) is one of the significant post-harvest diseases in fruit crops such as papaya (Carica papaya, L.). calcium chloride (CaCl2) concentration potentially reduces the infection of Colletotrichum gloeosporioides in papaya fruit coating, the design used in this study was Complete Randomized Design (CRD) using 5 treatments of CaCl2 (0, 2, 4, 6 and 8%) with 5 replications. The study was conducted at the Department of Plant Protection, Faculty of Agriculture, Universitas Padjadjaran, Indonesia, from April to July 2020. different concentrations of calcium chloride were sprayed on the surface of papayas with anthracnose symptoms. The symptoms, signs of the disease, and the intensity of the disease were observed after treatment. The intensity of the disease attack on the tenth day after the application showed non-significant results. The in vivo trial showed that the calcium chloride concentration of 8% could inhibit the growth of C. gloeosporioides by up to 20.93%. The papayas treated with CaCl2 at a concentration of 8% were entirely infected on the fifth day after application, while those treated with 2, 4 and 6% CaCl2 and control were infected on the third day. The calcium chloride 8% suppresses the incidence and severity of anthracnose.

Effect of calcium chloride on reducing Colletotrichum gloeosporioides (Penz.) Sacc. infection on post-harvest fruits of Carica papaya L. ​

Anthracnose (Colletotrichum gloeosporioides) is one of the significant post-harvest diseases in fruit crops such as papaya (Carica papaya, L.). calcium chloride (CaCl2) concentration potentially reduces the infection of Colletotrichum gloeosporioides in papaya fruit coating, the design used in this study was Complete Randomized Design (CRD) using 5 treatments of CaCl2 (0, 2, 4, 6 and 8%) with 5 replications. The study was conducted at the Department of Plant Protection, Faculty of Agriculture, Universitas Padjadjaran, Indonesia, from April to July 2020. different concentrations of calcium chloride were sprayed on the surface of papayas with anthracnose symptoms. The symptoms, signs of the disease, and the intensity of the disease were observed after treatment. The intensity of the disease attack on the tenth day after the application showed non-significant results. The in vivo trial showed that the calcium chloride concentration of 8% could inhibit the growth of C. gloeosporioides by up to 20.93%. The papayas treated with CaCl2 at a concentration of 8% were entirely infected on the fifth day after application, while those treated with 2, 4 and 6% CaCl2 and control were infected on the third day. The calcium chloride 8% suppresses the incidence and severity of anthracnose.

Effect of calcium chloride on heat-induced Mesona chinensis polysaccharide-whey protein isolation gels: Gel properties and interactions

Salt ions play an important role in the quality structure of food and sensory properties. This study aims to investigate the effects of different concentrations of calcium chloride (0–0.012 mol/L) on the gel properties and interactions of heat-induced Mesona chinensis polysaccharide (MCP)-whey protein isolate (WPI) mixed gels. It was found that the apparent viscosity decreases gradually with the increase of calcium ion concentration, especially when the calcium ion concentration reaches 0.012 mol/L, the apparent viscosity is the lowest. The water holding capacity of the mixed gel decreased from 86.25% to 32.05% and the network structure of the gel became looser, which was consistent with low-field magnetometry results and the variation of microstructure. In the in vitro digestion experiments, the addition of 0.003 mol/L calcium chloride was effective in reducing the degradation of the gel. The results of chemical bonding analysis indicated that hydrogen bond and hydrophobic interactions were the key forces that promoting gel formation and maintaining the gels network structure. This study showed that calcium ions could change the contribution of hydrogen bonding, disulfide bonds, and hydrophobic interactions to the gelation process.

Development of ATP13A2-deficient In vitro Model for PARK9 Parkinson’s Disease

Background: PARK9 familial Parkinson’s disease (PD) is caused by a loss-of-function mutation in the ATP13A2 gene in which the mutation impairs the autophagic-lysosomal degradation pathway and induces intraneuronal accumulation of alpha-synuclein. RNA interference has been a useful tool in generating in vitro knockdown model to study the physiological role of the gene. However, the availability of a validated ATP13A2-deficient in vitro model is limited. Objective: This study aimed to develop the ATP13A2-deficient PD model by delivering ATP13A2 siRNA into neuroblastoma cells using carbonate apatite nanoparticles (CA NPs). Methods: CA NPs were fabricated using different concentrations of calcium chloride and characterised in the presence or absence of ATP13A2 siRNA. Time-dependent stabilities of CA NPs and CA NPs-associated siRNA (CA-siRNA) complex were evaluated by pH, turbidity, size, and zeta potential measurements. The dissolution abilities at acidic conditions of both complexes were investigated. Following that, green fluorescence protein (GFP) and four different siRNAs targeting ATP13A2 (siRNA_5, 6, 7, and 8) were transfected to cells with the fabricated CA NPs. Western blot was performed to determine the knockdown effect of the four siRNAs. Results: It was found that 4 mM calcium chloride was ideal for CA NP formation, while an incubation time of 48 hours was required to maintain the stability of nanoparticles. Successful transfection was confirmed by detection of fluorescence signal from the GFP plasmid and the subsequent silencing of this signal by transfecting GFP siRNA. Western blot analysis revealed that ATP13A2 protein expression was significantly reduced to 20% upon transfection with 20 nM of siRNA_5. Conclusion: ATP13A2-deficient PD model was successfully developed.

In Vitro Ortamda Kalsiyum Klorürün Osteoblast Benzeri Hücreler Üzerine Etkisi

The aim of the study was to determine whether calcium chloride affects the proliferation of osteoblast like cells in a sex-dependent manner, as well as to determine the most effective concentration on proliferation of osteoblast like cells, in in vitro conditions. Bone marrow was used as biological material from young adult rats, both sexes, aged 90-95 days. Six different concentrations of calcium chloride were tested, determining the numerical representation of osteoblast like cells after 24 and 48 hours. Test results of mean values between males and females after 24 hours, indicate significant differences with a probability of P

QUATERNION-TYPE REPRESENTATION FOR MEASURING THE RENNET COAGULATION TIME OF MILK BY COLOR IMAGE SEQUENCES PROCESSING

The determination of the rennet coagulation time of milk by image sequences processing was performed using a computer vision system (CVS), consisting of a computer coupled with a transmitted light microscope equipped with a digital camera. Algorithms implemented with Matlab 2014 encoded the color image as a quaternion, computed and analyzed the histogram peak. The evolution of this last parameter was monitored as a function of the milk coagulation time, for different concentrations of calcium chloride (0.01–0.03 M) and for coagulation temperatures varying between 30 and 36°C. No statistically significant difference was observed in the measurements of the rennet coagulation time, neither by the Berridge method nor by the analysis of the image sequences, except for those at 36°C. The association of the optical microscopic method and the analysis of image sequences by a quaternion-type representation, made it possible to identify optical changes during gel formation and to accurately determine the rennet coagulation time of milk.

Milk fortified with calcium: Changes in the physicochemical and rheological characteristics that affect the stability

The objective of this work was to analyze the changes in the physicochemical and rheological characteristics of milk fortified with different calcium salts. Reconstituted milk samples using skim milk powder with different concentrations of calcium chloride and calcium lactate (0, 5 and 30 mmol kg−1) were obtained. Several physicochemical and rheometric techniques were used to analyze the effect of milk fortification. According to the results, all the applied techniques indicated that some of the added calcium migrates into the casein micelle forming colloidal calcium phosphate, and that the calcium added as lactate enters the micelles to a greater extent. A part of whey proteins would also be integrated into the micellar structure. An addition of 5 mmol kg−1 of calcium chloride and calcium lactate would be practically feasible, due to the mineral balance and the thermal stability that were not significantly affected at that concentration level. In conclusion, the results obtained with physicochemical techniques commonly used in literature are in agreement with those obtained in this study by rheometry, demonstrating that this simple and rapid technique allows inferring about the changes in mineral balance and effects on thermal stability when different salts are used for milk fortification.

A nondestructive contactless technique to assess the viscoelasticity of blood clots in real-time

There is a need for reliable and quantitative real-time assessment of blood properties to study and treat a broad spectrum of disorders and cardiovascular diseases as well as to test the efficacy of hemostatic agents. In this study, the real-time changes in viscoelastic/rheological properties of bovine whole blood during coagulation induced by different concentrations of calcium chloride (CaCl2; 15, 25, 35 and 45 mM) was investigated. For this purpose, a novel, contactless technique was used to accurately measure the clotting characteristics under controlled and sterile conditions. It was demonstrated that, increasing the calcium concentration from low values (i.e., 15 and 25 mM), led to shorter reaction time; however, a further increase in calcium concentration (i.e., 35 and 45 mM) favored longer reaction times. Additionally, increasing the CaCl2 concentration resulted in higher shear storage modulus (i.e., stiffer clots). These results were also comparable to those generated by thromboelastrograph, a clinically established technique, as well as a conventional rheometer, which quantitatively verified the high correlation of the shear storage modulus data. In sum, the non-destructive testing technique used in this study is reproducible and sensitive in measuring clot formation kinetics, which could be applied to assess the efficacy of hemostatic agents, and may also contribute to better diagnosing relevant circulatory system diseases and conditions.

A simple, non-toxic method for separating seeds based on density, and its application in isolating Arabidopsis thaliana seed oil mutants.

PremiseSeed oil is an economically important trait in Brassica oilseed crops. A novel method was developed to isolate Arabidopsis thaliana seeds with altered oil content.Methods and ResultsIn A. thaliana, seed oil content is correlated with seed density, with high‐oil seeds being less dense than wild type and tending to float in solution, and low‐oil seeds being denser and tending to sink. In contrast to previous methods, which used toxic chemicals and density gradient centrifugation, different concentrations of calcium chloride (CaCl2) were employed to separate seeds without the need for centrifugation. The method was validated using known seed oil mutants, and 120,822 T‐DNA mutagenized A. thaliana lines were then screened for novel seed density phenotypes.ConclusionsA number of candidate mutants, as well as new alleles of two genes known to influence seed oil biosynthesis, were successfully isolated.

Combination effects of calcium chloride and nano-chitosan on the postharvest quality of strawberry (Fragaria x ananassa Duch.)

Abstract This study aimed to investigate influences of calcium chloride (CaCl2) concentrations combined with nano-chitosan coating on the quality of strawberry during postharvest storage. The fruit were dipped in different concentrations of calcium chloride (1 %, 2 %, 3 %, 4 %) before being coated with 0.2 % nano-chitosan. Physico-chemical analysis including the overall quality index, weight loss, firmness, titratable acidity, total soluble solid, l -ascorbic acid content, antioxidant capacity, total phenolic content, total anthocyanin content, and malondialdehyde content were performed in 3 d intervals until fruit became unmarketable. Among six examined treatments, a combination of 3 % CaCl2 and nano-chitosan (NCTS) was the most effective one as maintaining the highest score of overall quality index of strawberry stored at 4 °C up to 15 d. The treatment also significantly reduced weight loss, preserved l -ascorbic acid, total anthocyanin contents, antioxidant capacity, and retarded malondialdehyde production. The scanning electron microscope image showed a smooth surface of strawberries coated with 3 % CaCl2 combined 0.2 % nano-chitosan. There has no bitterness detected in the treated strawberries after being stored 15 d at 4 °C. The major volatile compounds determined in the initial day were remained until the 15th d of storage.

Monitoring soil pH variation using Polyaniline/SU-8 composite film based conductometric microsensor

Development of Poly-aniline/SU-8 (an epoxy resin) nanocomposite film based conductometric microsensor platform to measure change in soil pH is reported. For this, poly-aniline doped with dodecylbenzene sulfonic acid (PANI-DBSA) was mixed with SU-8, and spun coated on Au-IDE patterned on Si/SiO2. The nanocomposite exhibited excellent response towards change in pH against three different conditions, namely standard buffer solutions with pH ranging from 2 to 10, Red and Bentonite soils, wherein the pH varied with water (soil moisture contents) and pH with different concentrations of calcium chloride (CaCl2) solution. The films exhibited the sensitivities of 455 and 290 μS/pH towards acidic (Lower pH) and alkaline solutions (Higher pH), respectively. The sensitivities of 34 and 89 μS/pH with water for Red and Bentonite soil samples, respectively were obtained. Importantly, for Red and Bentonite soils, the sensitivities of 41 and 52 μS/pH with CaCl2 concentration were achieved. Moreover, the sensor revealed faster response kinetics with response and recovery times of ˜10 and ˜30 s respectively. Overall, the sensor exhibited good sensitivity, excellent long term stability, high repeatability and low fabrication cost. Our results demonstrated that PANI/SU-8 composite films as a potential material for the determination of soil pH variation crucial for precision agriculture.

Synchronizing the release rates of salicylate and indomethacin from degradable chitosan hydrogel and its optimization by definitive screening design

Three types of ionically crosslinked (with citric acid) chitosan discs were loaded with the highly water- soluble drug, sodium salicylate (SS) and the poorly water-soluble drug, indomethacin (Ind). In separate experiments the hydrated discs were immersed in a de-crosslinking solution comprising of different concentrations of calcium chloride, which induced a controlled erosion of the discs, a process which was optimized to synchronize the release rates of the two drugs over a predetermined period of time. The optimization was accomplished by manipulating six factors: chitosan MW, its amount in the formulation, the concentration of the crosslinker agent, the concentration of the de-crosslinking agent in the dissolution medium, its pH and its temperature. A computerized multifactorial definitive screening design analysis assisted in minimizing the number of experiments. The quotient of the SS to Ind release rates, the difference factor f1, the similarity factor f2 and the combination of f1 and f2 were determined as the experimental responses. The computerized prediction profilers that were used to simulate the contribution of the experimental factors and their effect on the experimental responses led to a successful erodible formulation with a concomitant release of the two drugs over 150 min.

FORMULATION AND EVALUATION OF DIAZEPAM-LOADED INTRAVAGINAL ALGINATE BEADS: AN INVESTIGATION STUDY FOR THE TREATMENT OF PELVIC FLOOR DYSFUNCTION

Objective: The fundamental objective of this research investigation was to develop intravaginal diazepam (DZ)-loaded alginate beads for the management of acute pelvic floor dysfunction (PFD) pain with minimal sedative effect.Methods: DZ loaded beads were prepared by an ionotropic gelation method using SA (sodium alginate) alone, or in combination with either poloxamer 407 (PL), pectin (PC), or xanthan gum (XG) at different ratios in the presence of different concentrations of calcium chloride as a cross-linking agent. The successfully developed beads were evaluated for the particle size, pH, yield percentage, entrapment efficiency, in vitro bioadhesion, swelling percentage, and in vitro drug release. The stability, ex vivo drug permeation, and sedative action of the optimized beads formulations were studied.Results: The particle size of the formulated beads was from 395±3.3 to 515±2.8 μm, yield percentage was from 68.2±1.7 to 87.5±2.1, entrapment efficiency was from 65.6±1.6 to 87.5±2.1. pH ranged from 6.1±0.2 to 6.8±0.6, bioadhesion strength was from 71.5±1.3 to 87.6±3.1, and swelling percentage was in the range from 53.4±3.1 to 85.2±3.7. Approximately 92.4–72.6% of the loaded dose was released from the prepared beads. The optimized beads showed a good stability under the selected storage conditions. About 74.8%, 71.1%, 68.6%, and 63.4% of the loaded dose permeated through the rabbit vaginal mucosa from F7, F9, F3, and F11, respectively. The formulated beads decreased the sedative action associated with orally or parenterally administered DZ.Conclusion: The developed beads were considered a promising candidate to formulate DZ into a new dosage form for the treatment of PFD with a minimum central nervous system sedation.

Development of a thresholding algorithm for calcium classification at multiple CT energies

The objective of this study was to develop a thresholding method for calcium classification with different concentration using single-energy computed tomography. Five different concentrations of calcium chloride were filled in PMMA tubes and placed inside a water-filled PMMA phantom (diameter 10 cm). The phantom was scanned at 70, 80, 100, 120 and 140 kV using a SECT. CARE DOSE 4D was used and the slice thickness was set to 1 mm for all energies. ImageJ software inspired by the National Institute of Health (NIH) was used to measure the CT numbers for each calcium concentration from the CT images. The results were compared with a developed algorithm for verification. The percentage differences between the measured CT numbers obtained from the developed algorithm and the ImageJ show similar results. The multi-thresholding algorithm was found to be able to distinguish different concentrations of calcium chloride. However, it was unable to detect low concentrations of calcium chloride and iron (III) nitrate with CT numbers between 25 HU and 65 HU. The developed thresholding method used in this study may help to differentiate between calcium plaques and other types of plaques in blood vessels as it is proven to have a good ability to detect the high concentration of the calcium chloride. However, the algorithm needs to be improved to solve the limitations of detecting calcium chloride solution which has a similar CT number with iron (III) nitrate solution.