Different Concentrations Of Alcohol Research Articles

Role of Ferroptosis Regulation by Nrf2/NQO1 Pathway in Alcohol-Induced Cardiotoxicity In Vitro and In Vivo.

The aim of the present study was to evaluate the cardiotoxic effects of alcohol and its potential toxic mechanism on ferroptosis in mice and H9c2 cells. Mice were intragastrically treated with three different concentrations of alcohol, 7, 14, and 28%, each day for 14 days. Body weight and electrocardiography (ECG) were recorded over the 14 day period. Serum creatine kinase (CK), lactic dehydrogenase (LDH), MDA, tissue iron, and GSH levels were measured. Cardiac tissues were examined histologically, and ferroptosis was assessed. In H9c2 cardiomyocytes, cell viability, reactive oxygen species (ROS), labile iron pool (LIP), and mitochondrial membrane potential (MMP) were measured. The proteins of ferroptosis were evaluated by the western blot technique in vivo and in vitro. The results showed that serum CK, LDH, MDA, and tissue iron levels significantly increased in the alcohol treatment group in a dose-dependent manner. The content of GSH decreased after alcohol treatment. ECG and histological examinations showed that alcohol impaired cardiac function and structure. In addition, the levels of ROS and LIP increased, and MMP levels decreased after alcohol treatment. Ferrostatin-1 (Fer-1) protected cells from lipid peroxidation. Western blotting analysis showed that alcohol downregulated the expression of Nrf2, NQO1, HO-1, and GPX4. The expressions of P53 and TfR were upregulated in vivo and in vitro. Fer-1 significantly alleviated alcohol-induced ferroptosis. In conclusion, the study showed that Nrf2/NQO1-dependent ferroptosis played a vital role in the cardiotoxicity induced by alcohol.

Alcohol induces ER stress and apoptosis by inducing oxidative stress and disruption of calcium homeostasis in glial cells

Alcohol has teratogenic effects that can cause developmental abnormalities and alter anatomical and functional characteristics of the developed brain and other organs. Glial cells play a crucial role in alcohol metabolism and protect neurons from toxic effects of alcohol. However, chronic alcohol exposure can lead to uncontrollable levels of reactive oxygen species, resulting in the death of glial cells and exposing neuronal cells to the toxic effects of alcohol. The exact molecular mechanism of alcohol-induced glial cell death has not been fully explored. This study reported that different concentrations of alcohol induce different expressions of ER stress markers in glial cells, focusing on the role of endoplasmic reticulum (ER) stress. Alcohol-induced concentration-dependent toxicity in both cells also induced oxidative stress, leading to mitochondrial damage. The expression of p53 and apoptotic proteins was significantly up-regulated after alcohol exposure, while Bcl2 (anti-apoptotic) was down-regulated. The signalling pathway for ER stress was activated and up-regulated marker proteins in a concentration-dependent manner. Cells pre-treated with BAPTA-AM and NAC showed significant resistance against alcohol assault compared to other cells. These in vitro findings will prove valuable for defining the mechanism by which alcohol modulates oxidative stress, mitochondrial and ER damage leading to glial cell death.

Effects of alcohol on the symptoms of gouty arthritis and taxonomic structure of gut microbiota in C57BL/6 mice.

Gout is an acute arthritis caused by the elevated levels of serum uric acid (UA), and its prevalence has been rapidly increasing. Alcohol abuse could lead to a series of health problems. Multiple pieces of evidence suggest that alcohol intake affects the development and progression of gout, while the gut microbiota plays an important role in the development of gout and the long-term alcohol consumption could affect the stability of the gut microbiota. This study aimed to explore the effects of alcohol intake at different concentrations on gouty arthritis based on the gut microbiota. We investigated the effects of different concentrations of alcohol on gouty arthritis in mouse models of acute gouty arthritis established by injection of monosodium urate (MSU) crystals into C57BL/6 mice. The results indicated that the high-alcohol consumption not only exacerbated joint swelling and pain, increased the levels of UA, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), but also showed dramatic effects on the composition and structure of the gut microbiota in gouty mice. Two key microorganisms, Parasutterella and Alistipes, could aggravate gout symptoms through lipopolysaccharide biosynthesis, riboflavin metabolism, phenylalanine metabolism, and arginine and proline metabolisms. In conclusion, our study suggested that high-concentrations of alcohol altered the gut microbiota structure in gouty mice induced by MSU crystals, which could exacerbate gouty symptoms by enhancing pro-inflammatory pathways.

Isolation, Identification and Leavening Ability of Yeast from Local Fruits

Yeast is the organism used in the leavening of dough in a bakery. The study was carried out to determine the leavening ability of yeast isolated from different fruits. Three different yeast species were isolated from different fruits and were identified using cultural and microscopic methods. The yeast species isolated were tested for their temperature tolerance, ethanol tolerance, carbohydrate fermentation and leavening ability. The isolates were able to tolerate different temperature ranges (25 °C, 30 °C and 37 °C), and different concentrations of alcohols (2%, 4%, 6%, and 8%). They were also able to grow and ferment glucose, sucrose, and fructose. The ability of the identified yeast species to increase the volume of the dough was examined by fermenting flour dough. The fermentation was conducted at 30 °C for 72 h. Yeast isolates from date, pineapple, mango and commercial beakers yeast were found to have a leavening ability of 66, 60, 40 and 58, respectively, and the control having 20 cm3/g. This indicates that the fruits could be a potential source of indigenous yeast species which can serve as good leavening agents.

Dissecting operant alcohol self-administration using saccharin-fading procedure.

Although alcohol use disorder is a complex human pathology, the use of animal models represents an opportunity to study some aspects of this pathology. One of the most used paradigms to study the voluntary alcohol consumption in rodents is operant self-administration (OSA). In order to facilitate the performance of this paradigm, we aim to describe some critical steps of OSA under a saccharin-fading procedure. We used 40 male Wistar rats to study the process of acquiring the operant response through a saccharin-fading procedure under a fixed ratio (FR1) schedule of reinforcement. Next, we analyze the alcohol introduction and concentration increase, the effect of an alcohol deprivation, and the analogy between this paradigm with the Drinking in the Dark-Multiple Scheduled Access paradigm. During alcohol concentration increase, animals reduced their lever presses in accordance with the increase in alcohol concentration. On the contrary, the consumption measured in g·kg-1 BW showed a great stability. The lever presses pattern within operant session changes with the introduction of different alcohol concentrations: at higher alcohol concentrations, animals tended to accumulate most of their presses in the initial period of the session. We show the utility of fading with low concentrations of saccharin and the evolution of the operant response through the different concentrations of alcohol. Taken together, our results aimed to dissect the acquisition and maintenance of OSA behavior as well as other related variables, to facilitate the understanding and performance of this paradigm.

Chronic Alcohol Reduces Bone Mass Through Inhibiting Proliferation and Promoting Aging of Endothelial Cells in Type-H Vessels

Alcohol consumption is regarded as one of the leading risk factors for secondary osteopenia. Angiogenesis and osteogenesis coupled by type-H vessels coordinate the biological process of bone homeostasis to prevent osteopenia. This study aimed to determine whether chronic alcohol inhibits type-H vessel-dependent bone formation. Two-month-old mice were fed with 5% (v/v) alcohol liquid diet (28% of calories) or normal liquid diet every day for 2 months. The tibias were isolated and detected with X-ray and microcomputed tomography. Paraffin-embedded or frozen tibial sections were prepared and used for immunohistochemical or immunofluorescence staining, respectively. Human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of alcohol, including 0 mM (0%), 8.7 mM (0.5%), 52 mM (3%), or 87 mM (5%) alcohol for 12 h. The conditioned medium of the above HUVEC cells was collected to culture human bone marrow-mesenchymal stem cells (BM-MSCs), which were induced to differentiate into osteoblasts in vitro. The alcoholic diet retarded the bone growth and led to osteoporosis, impaired bone formation of osteoblasts, and decreased CD31hiEMCNhi type-H vessel formation through inhibiting proliferation and promoting aging of endothelial cells in mice. Alcohol treatment obviously increased the expression of p16, while significantly decreased the expression of Bmi-1, CDK6, Cyclin D, E2F1, and bone morphogenetic protein (BMP)2 compared with vehicle. Alcohol inhibited the differentiation of BM-MSCs into osteoblasts through reducing the BMP2 secretion of endothelial cells in type-H vessels. Alcoholic diet impaired CD31hiEMCNhi type-H vessel formation through inhibiting proliferation and promoting aging of endothelial cells through Bmi-1/p16 signaling, and inhibited the differentiation of BM-MSCs into osteoblasts through reducing the BMP2 secretion of endothelial cells in type-H vessels. This study provides a basis for developing a new treatment strategy targeting aging endothelial cells of type-H vessel to prevent alcoholic osteopenia.

Comparitive evaluation of different concentrations of alcohol in ultrasound guided coeliac plexus neurolysis for pain relief in upper abdominal malignancies

Patients suffering from advanced upper abdominal malignancies have pain as predominant symptom affects their quality of life and survival. USG guided coeliac plexus neurolysis become benevolence in these patients on part of their pain management and quality of life improvement. To compare the efficacy of USG guided coeliac plexus neurolysis for pain relief in upper abdominal malignancies by using different concentration of alcohol (50% vs 75%).This Prospective, comparative, randomised double blinded study was conducted during Sep 2019 – Aug 2020 at our tertiary care centre. Total 60 cases were taken as per following inclusion and exclusion criteria and randomly divided into 2 groups i.e. 30 each group, we compare Visual Analogue Scale (VAS) score, quality of life (QOL) and need of rescue analgesia profile between the groups to know the efficacy of USG guided coeliac plexus block. In our study, we observed that the baseline mean VAS score in group I was 8.26±0.78 while in group II was 8.03±0.76. No significant difference was found in mean VAS score at this time between the groups (p=0.24). The baseline mean QOL score in group-I was 77.46±3.40 while for the cases of group II the mean QOL score was 77.36±3.33. No significant difference was found in mean QOL score at baseline between the groups (p=0.90). The baseline mean morphine consumption in group-I was 113.33±39.24 mg while for the cases of group-II the mean morphine consumption was 120.33±38.37mg. No significant difference was found in mean morphine consumption at this time between the groups (p=0.48).Both groups having 50% alcohol and 75% alcohol decreases the VAS score from baseline in patients having upper abdominal malignancies along with QOL and dosages of rescue analgesia whereas no significant difference in VAS score in patients of both groups.

Improvement effect and mechanism of ethanol extract from Citri Reticulatae Pericarpium on triglyceride in hyperlipidemia model rat

The aim of this paper was to study the improvement effect of ethanol extract from Citri Reticulatae Pericarpium(CRP) on triglyceride of hyperlipidemia model rats, and to explore the possible mechanism. SD rats were randomly divided into normal group, model group, positive control group, and high, medium and low-dose CRP ethanol extract groups, with 10 rats in each group. During the experiment, except for the normal group that was fed with distilled water and ordinary feed, rats in the other groups were given different concentrations of alcohol and fed with high-sugar and fat diets. All rats were given free diets. While being modeled, each group was administered with 0.01 mL·g~(-1) by gavage once a day for six weeks. Blood samples were collected after two weeks, four weeks and six weeks of drug treatment. After the completion of the experiment, blood, liver and adipose tissue were collected. Triglyceride(TG), alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) in serum, TG in liver tissue and TG in fecal were detected. Free fatty acid(FFA) and triglyceride-related hydrolase, such as adipose tiglyceride lipase(ATGL), lipoprotein lipase(LPL), hepatic lipase(HL), hormone-sensitive triglyceride lipase(HSL) were detected by ELISA. The mRNA expressions of peroxisome proliferators-activated receptors(PPARγ), sterol regulatory element binding protein 1 c(SREBP-1 c) and farnesoid X receptor(FXR) were determined by RT-PCR. Compared with the model group, each administration group could reduce TG levels in serum and liver to varying degrees, reduce serum ALT, AST, ALP activities, significantly reduce free fatty acid content in serum, significantly increase triglyceride metabolism-related enzymes, including fat ATGL, LPL and liver HL content, and significantly reduced the content of fat HSL. According to the study of transcriptional regulation genes relating to triglyceride metabolism, extract from CRP could significantly increase the mRNA expressions of PPARγ and FXR. In conclusion, ethanol extract from CRP could ob-viously reduce the TG level of hyperlipidemia model rats, and might reduce plasma TG content by increasing PPARγ-LPL/ATGL and FXR-HL triglyceride hydrolysis pathways.

Evaluación de la estabilidad microbiológica del pan de molde integral mediante el uso de natamicina

En los últimos años existe un interés creciente por ingerir alimentos naturales, es decir, que sean mínimamente procesados. La bioconservación satisface estos requisitos, ya que aprovecha la capacidad de microorganismos reconocidos como seguros (GRAS) y/o de sus metabolitos para inhibir el desarrollo de microorganismos alterantes o patógenos en alimentos. En el sector panadero el principal inconveniente es la conservación del pan luego del horneado, ya que por la composición y la temperatura de almacenamiento son muy propensos al ataque por hongos. La finalidad de la presente investigación fue evaluar la estabilidad microbiológica del pan de molde integral aplicando por aspersión, soluciones de natamicina con diferentes concentraciones de alcohol en la superficie del panificado a 65°C y 30°C. El estudio implicó análisis microbiológicos de mohos y levaduras, determinaciones de humedad y una evaluación sensorial mediante una prueba triangular, con 24 jueces semi entrenados. Se siguieron las metodologías descritas en los métodos de la AOAC e ISO. El análisis de humedad y el recuento de mohos y levaduras muestran que no existen diferencias significativas (P<0.05) entre los tratamientos. En cuanto al análisis sensorial se evidencia que no existen diferencias significativas en el sabor. Estos resultados demuestran que el pan de molde integral mantiene su estabilidad microbiológica durante los 18 días de estudio siendo el uso del bioconservante una respuesta para los consumidores que continuamente buscan productos más frescos y con menor cantidad de aditivos químicos.

Chronic consumption of alcohol increases alveolar bone loss.

This study evaluated the effects of the chronic consumption of different concentrations of alcohol on the experimental periodontitis (EP). 160 rats were divided into 4 groups: (EP-NT) rats with EP and no alcohol exposure; (EP-A14) rats with EP exposed to 14% alcohol; (EP-A25) rats with EP exposed to 25% alcohol; (EP-A36) rats with EP exposed to 36% alcohol. The animals from the EP-A14, EP-A25 and EP-A36 groups were subjected to different concentrations of alcohol 30 days before EP induction. The histological characteristics, percentage of bone in the furcation (PBF) and bone metabolism in the furcation region were evaluated. The PBF and tartrate-resistant acid phosphatase (TRAP) data were subjected to statistical analysis. The EP-A14, EP-A25 and EP-A36 groups had lower PBFs compared with the EP-NT group. A more severe inflammatory process and a greater number of TRAP+ cells were also observed. In the EP-A14, EP-A25 and EP-A36 groups, the inflammatory process became more severe as the ingested alcoholic concentration increased. An increase in RANKL immunolabeling and a significantly higher number of TRAP+ cells were also observed. We conclude that chronic alcohol consumption increases the severity of experimental periodontitis in a dose-dependent manner by increasing the magnitude of local inflammatory responses and stimulating alveolar bone resorption.

Abstract 1856: Effects of different ethanol elutefractions of Qigesan, Tongyoutang, Shashenmaidongtang and Buqiyunpitang on expression of secretory proteins in esophageal carcinoma EC9706cell

Abstract Purpose: To investigate the effect of different concentration of ethanol elution of Chinese medicine prescriptions(Qigesan, Tongyoutang, Shashenmaidongtang and Buqiyunpitang) on the proliferation, cell cycle and MMP1, MMP11, PCSK9, TREM2, STX1A secretory proteins expression in esophageal cancer EC9706 cells line, so as to explore the connotation of TCM syndrome pathogenesis and molecular biology. Methods: Water extracts of Qigesan, Tongyoutang, Shashenmaidong tang and Buqiyunpitang were made gradient elution and got different concentration of ethanol elution fractions by macroporous resin, ultrapure water, 30% ethanol, 50% ethanol, 70% ethanol, and anhydrous ethanol. MTT methods was used to screen the the above four prescriptions for inhibiting the proliferation of EC9706 cell. The effects of most obvious elution fractions of esophageal four prescriptions on the proliferation, cell cycle and secretory proteins expression were investigated by MTT assay, flow cytometry and Western blot analysis, respectively. Results: Different concentration of ethanol elution fractions of Qigesan, Tongyoutang, Shashenmaidong tang and Buqiyunpitang were got through macroporous resin D101 gradient elution with different concentrations of alcohol. 30% ethanol elution fraction of Qigesan, 50% ethanol elution fraction of Tongyoutang and Sashenmaidongtang, anhydrous ethanol elution fractions of Buqiyunpitang had obviously inhibitory effect on the proliferation of EC9706 cell in dose-dependent and time-dependent manner, changed morphology of EC9706 cell, which appeared as round with rough edges, karyopyknosis, and karyorrhexis. Ic50 values of Qigesan 30% ethanol elution fraction, Tongyoutang 50% ethanol elution fraction, Sashenmaidongtang 50% ethanol elution fraction, Buqiyunpitang anhydrous ethanol elution fractions for EC9706 were 825.09ug·ml-1,860.66 ug·ml-1, 270.43 ug·ml-1, 634.60 ug·ml-1, which all induced G0/G1 phase arrest in EC9706 cells. Qigesan 30% ethanol elution fractions could inhibit the expression of MMP1, MMP11, TREM2, IGFPB3; Tongyoutang 50% alcohol fractions could inhibit STX1A expression; Sashenmaidongtang 50% ethanol elution fractions could inhibit MMP1, PCSK9, TREM2, IGFPB3, STX1A expression; Buqiyunpitang anhydrous ethanol fractions could inhibit MMP1, MMP11, PCSK9, TREM2, IGFPB3 and STX1A expression. Conclusion: Qigesan 30% ethanol elution fractions, Tongyoutang and Sashenmaidongtang 50% ethanol elution fractions, Buqiyunpitang anhydrous ethanol elution fractions can inhibit the proliferation of EC9706 cell, which have close relationship with MMP1, MMP11, PCSK9, TREM2, IGFPB3, STX1A proteins expression. This study provides new basis for the development of anti-esophageal carcinoma drugs in traditional Chinese medicine. Key words: esophageal carcinoma; Qigesan; Tongyoutang; Shashenmaidongtang; Buqiyunpitang; secretory protein Citation Format: Fuchun Si. Effects of different ethanol elutefractions of Qigesan, Tongyoutang, Shashenmaidongtang and Buqiyunpitang on expression of secretory proteins in esophageal carcinoma EC9706cell [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1856.

Susceptibility of Tick-Borne Encephalitis Virus to Inactivation by Heat, Acidic pH, Chemical, or UV Treatment.

Tick-borne encephalitis virus (TBEV) is a single-stranded, positive-sense RNA virus in the family Flaviviridae that is endemic in parts of Europe and Asia and can cause meningitis or encephalitis. Due to the disease severity, TBEV requires handling under heightened biosafety measures. The establishment and validation of inactivation procedures is a prerequisite for downstream analyses and management of occupational exposure. Therefore, different procedures for TBEV inactivation were tested. Our results suggest that TBEV is susceptible to inactivation by heat, acidic pH, different concentrations of alcohol, formaldehyde, or detergents, and exposure to UV irradiation, which may depend on sample size and composition.

Noninvasive Suspicious Liquid Detection Using Wireless Signals.

Conventional liquid detection instruments are very expensive and not conducive to large-scale deployment. In this work, we propose a method for detecting and identifying suspicious liquids based on the dielectric constant by utilizing the radio signals at a 5G frequency band. There are three major experiments: first, we use wireless channel information (WCI) to distinguish between suspicious and nonsuspicious liquids; then we identify the type of suspicious liquids; and finally, we distinguish the different concentrations of alcohol. The K-Nearest Neighbor (KNN) algorithm is used to classify the amplitude information extracted from the WCI matrix to detect and identify liquids, which is suitable for multimodal problems and easy to implement without training. The experimental result analysis showed that our method could detect more than 98% of the suspicious liquids, identify more than 97% of the suspicious liquid types, and distinguish up to 94% of the different concentrations of alcohol.

Effects of Ringer's solution with different concentrations of alcohol on biphasic compound action potentials of frog sciatic nerve trunk

To quantitatively investigate the effects of Ringer's solution with different concentrations of alcohol (1%～80%) on biphasic compound action potentials (AP) from frog sciatic nerve trunk, and their recoveries from alcohol effects. Individual segments of frog sciatic nerve trunk with a length of 6 to 8 cm were prepared. Ringer's solution with different concentrations of alcohol (0%, 1%, 2%, 4%, 8%, 16%, 32%, 48%, 64% and 80%) was applied onto the segment of the trunk between the stimulus and ground electrodes via an agent reservoir which was newly armed in a nerve trunk shielded chamber for 5 minutes. The nerve trunk was respectively electro-stimulated to generate the biphasic compound AP which was recorded using the experimental system of BL-420F. This was followed by 5 times washout plus 5 min administration with Ringer's solution before recovery recording of AP. Compared to normal Ringer's solution, Ringer's solution with alcohol at ≤4% did not have dramatic impacts on the AP amplitude and conduction velocity, while Ringer's solution with alcohol at ≥8% there was significant decrease in these two parameters. Ringer's solution with alcohol at the conentrations of 16%, 32% and ≥48% could prevent a small proportion (30%), a large proportion (90%) and all (100%) of sciatic nerve trunks, respectively, from generating AP. Washout with normal Ringer's solution after alcohol application at the concentration of ≤32%, AP could totally recover to normal status. While alcohol at the concentration of 48%, 64% and 80%, the probabilities to regenerate APs were 90%, 40% and 0%, and the AP amplitudes were decreased to 60%, 36% and 0%, respectively. After washout, AP conduction velocity showed no difference with alcohol at the concentration of ≤8% when compared with that before washout, while it could not be recovered to normal under alcohol at ≥16%. Ringer's solution with different concentrations of alcohol exerts different effects on biphasic compound AP amplitude and conduction velocity. Hopefully, our findings could be helpful for the alcoholic usage and its recovery from alcoholic damage.

Effect of Adding Different Concentrations of Alcohol Extracted Ginger as Feed Supplementation on Some Productive Traits of Broiler Chicken Ross Strain

This study was conducted in the poultry field that belongs to Animal Production Techniques Department / Al musiab Technical College. To investigate the effect of alcohol extracted ginger supplementation in the diet on some productive traits of broiler chicken ross strain. Two hundred fifty unsexed broiler chicks were equally divided into the five groups, 50 chicks per group, 2 replicates per group (25 chicks per replicate). Chicks were fed on starter diet (1 day- 3 week) and final diet (4-6 week), diets supplemented with five ratios from alcohol extracted ginger (0, 150, 200, 250, 300 mg/kg), the first group was control but the other represented experimental groups.
 Results showed a significantly improve (P≤0.05) of average live body weight, weight gain, feed conversion ratio and dressing percentage in birds supplemented with alcohol extracted ginger as compared with control group at three and six weeks of birds age. As supplementation of alcohol extracted ginger had significantly (P≤0.05) decreased feed consumption and mortality ratio in experimental groups as compared with the control group.
 It can be concluded that supplementation of alcohol extracted ginger to the diet had a positive effect to enhance productive traits of broiler chicken ross strain.

Alcohol Hangover: Underlying Biochemical, Inflammatory and Neurochemical Mechanisms.

To review current alcohol hangover research in animals and humans and evaluate key evidence for contributing biological factors. Narrative review with alcohol hangover defined as the state the day after a single episode of heavy drinking, when the alcohol concentration in the blood approaches zero. Many of the human studies of hangover are not well controlled, with subjects consuming different concentrations of alcohol over variable time periods and evaluation not blinded. Also, studies have measured different symptoms and use varying methods of measurement. Animal studies show variations with respect to the route of administration (intragastric or intraperitoneal), the behavioural tests utilised and discrepancy in the timepoint used for hangover onset. Human studies have the advantage over animal models of being able to assess subjective hangover severity and its correlation with specific behaviours and/or biochemical markers. However, animal models provide valuable insight into the neural mechanisms of hangover. Despite such limitations, several hangover models have identified pathological changes which correlate with the hangover state. We review studies examining the contribution of alcohol's metabolites, neurotransmitter changes with particular reference to glutamate, neuroinflammation and ingested congeners to hangover severity. Alcohol metabolites, neurotransmitter alterations, inflammatory factors and mitochondrial dysfunction are the most likely factors in hangover pathology. Future research should aim to investigate the relationship between these factors and their causal role.

Simultaneous purification of dihydrotanshinone, tanshinone I, cryptotanshinone, and tanshinone IIA from Salvia miltiorrhiza and their anti-inflammatory activities investigation

Dihydrotanshinone, tanshinone I, cryptotanshinone, and tanshinone IIA are major lipid-soluble constituents isolated from Salvia miltiorrhiza Bunge (Danshen). In the present study, a systematic method was developed to simultaneously isolate and purify those compounds using macroporous adsorption resins and semi-preparative HPLC with a dynamic axial compress (DAC) system. The Danshen extract (95% alcohol) was divided into three fractions using different concentrations of alcohol (0%, 45%, and 90%) on D101 column. The content of total tanshinones of 90% alcohol eluent (TTS) was over 97%. Furthermore, the anti-inflammatory effects of those samples were investigated on LPS-stimulated RAW264.7 cells and three animal models. The results showed that the anti-inflammatory effect of TTS in vitro was superior to the one of any other sample including 0% and 45% eluent, and total tanshinones capsules. In addition, TTS exhibited a stronger anti-inflammatory effect than that of dihydrotanshinone, tanshinone IIA, cryptotanshinone, and tanshinone I, respectively. For animal models, TTS could significantly suppress xylene-induced ear oedema and rescue LPS-induced septic death and acute kidney injury in mice. In summary, the separation process developed in the study was high-efficiency, economic, and low-contamination, which was fit to industrial producing. TTS is a potential agent for the treatment of inflammatory diseases.

Morphological and biochemical effects of weekend alcohol consumption in rats: Role of concentration and gender.

AIMTo examine the association between weekend alcohol consumption and the biochemical and histological alterations at two different concentrations of alcohol in both genders in rats.METHODSWistar rats weighing 170-200 g were divided into groups as follows: (1) Control groups; and (2) weekend alcohol-consumption group: 2 d/weekly per 12 wk, at two different concentrations: (1) Group of males or females with a consumption of a solution of alcohol at 40%; and (2) group of males or females with a consumption of a solution of alcohol at 5%. At the end of the experiment, serum and liver samples were obtained. The following enzymes and metabolites were determined in serum: Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Lactate Dehydrogenase, and Gamma-Glutamyltransferase, and glucose, triglycerides, cholesterol, bilirubin, and albumin. Liver samples from each group were employed to analyze morphological abnormalities by light microscopy.RESULTSIn all of the weekend alcohol-consumption groups, AST activity presented a significant, 10-fold rise. Regarding ALT activity, the groups with weekend alcohol consumption presented a significant increase that was six times greater. Bilirubin levels increased significantly in both groups of females. We observed a significant increase in the parameters of fatty change and inflammation due to weekend alcohol consumption. Only the group of females that consumed alcohol at 40% presented slight hepatocellular disorganizationCONCLUSIONThe results obtained herein provide solid evidence that weekend alcohol consumption gives rise to liver damage, demonstrated by biochemical and histological alterations, first manifested acutely, and prolonged weekend alcohol consumption can cause greater, irreversible damage.

Ethanol influence on gingival fibroblasts - areal-time in vitro study.

Alcohol consumption is the world's third largest risk factor for disease and disability. According to the WHO report from 2011: 71% of urban respondents ty and 77% of rural respondents admit to alcohol consumption]. Lower socio-economic status and educational levels result in a greater risk of alcohol-related injury, disease and death. Alcohol is a common component of many medicines, as well as an ingredient in many oral hygiene home products. Mouthwashes containing alcohol are considered to inhibit wound healing in the oral cavity. Due to the fact that many different results are described for different concentrations of alcohol at different times, an attemptwas made to visualise the direct impact of 7.2% and 22% alcohol on human gingival fibroblasts. PANsystem 2000 was used for visualisation of the reaction of human gingival fibroblasts isolated from gingiva on ethanol in 2 different concentrations. PANsys 3000 is a multi-system fully-automated cell culture device used for in vitro culture and to study a variety of cell lines under conditions similar to in vivo. Observations were carried out for 48 hours since alcohol addition. Pictures were taken in a continuous process at 5 minute intervalds and combined into a film. Both contamination of 7.2% and 22% ethyl alcohol negatively affected morphology and cell proliferation. Addition of ethanol at a concentration 7.2% enabled cells to regain their ability to divide and recover normal morphology after 10 hours; changes caused by 22% ethanol, however, were irreversible. The obtained results suggest that daily usage of 7.2% alcohol contained in mouthwashes is non-toxic for gingival fibroblasts, and could be recommended after periodontal surgery.

Sensing application of an optical fiber dip coated with L-Cystein ethyl ester hydrochloride capped ZnTe quantum dots

AbstractOptical fiber in conjunction with ZnTe quantum dots (QDs) is investigated for sensing application. ZnTe QDs, are synthesized by a simple chemical bottom up approach. Quantum dots are capped with L-Cystein ethyl ester hydrochloride (LEEH), to increase their stability. Then LEEH capped ZnTe QDs, whose size is estimated as 2.29 nm by effective mass approximation (EMA), are dip-coated on a cladding removed optical fiber. Different concentrations of alcohol and ammonia are used to investigate the sensing behavior. It is found that sensitivity of the sensor increases with the use of QDs for both alcohol and ammonia.