Different Agro-ecological Regions Of India Research Articles

Soil erosion in diverse agroecological regions of India: a comprehensive review of USLE-based modelling.

Soil erosion caused by water refers to the removal of topsoil by rainfall and runoff. Proper selection of an assessment method is crucial for quantifying the spatial variance of soil erosion. The Universal Soil Loss Equation (USLE) and its revised version (RUSLE) are widely used for modelling soil erosion. This study aimed to evaluate the effectiveness of the USLE-based soil erosion modelling in different agroecological regions of India, identify potential issues, and provide suggestions for future applications. The review revealed that little attention has been given to estimate soil erosion in high-priority land degradation regions of India. Additionally, many studies failed to thoroughly verify the authenticity of stated soil loss rates in their research regions either by overestimating or underestimating at least one of the five soil loss parameters. Furthermore, flaws in the application of methods to calculate these parameters leading to erroneous values were identified and suggestions for improvement were made. The USLE-based soil erosion modelling is an effective tool for quantifying soil erosion risk, but researchers should put emphasis on thoroughly verifying the methodologies adopted, unit conversions, and data availability for the estimation of soil loss parameters to improve the accuracy of their final results. This paper provides valuable insights to assist researchers in implementing USLE-based erosion models in diverse agroecological regions in India and elsewhere. However, for effective soil conservation and sustainable agriculture, further research is necessary to develop efficient techniques for using USLE-based soil erosion modelling to achieve a comprehensive understanding of erosion risk across different agroecological regions.

Spatio-temporal trend of crop phenology, SPEI, and their interactions over different agro-ecological regions of India

Spatio-temporal trend of crop phenology, SPEI, and their interactions over different agro-ecological regions of India

An Agroecological Analysis of Livestock Wealth in India

This study aims to analyze the livestock population dynamics, adoption pattern of livestock and infrastructure facilities across different agroecological regions of India. The results revealed that more than 50 per cent of all livestock species were found in rain-fed areas. In irrigated region, 36 per cent of the livestock population depended upon 15 per cent of the geographical area. The cattle population growth rate declined for all regions because of a faster decline in indigenous cattle in all the agroecological regions. The number of crossbred cattle increased from 26 to 44 million among the cattle population at the India level. It may be due to the acceptance of insemination techniques with the expectation of high milk yield. Whereas, in irrigated region, the situation was very different, with a marginal increase in the share of crossbred cows. The findings indicated that India’s large and uniquely structured livestock sector was growing with compound annual growth of 4.3 per cent. For the sustainable development of the sector, suitable efforts should be taken to improve the genetic potential of indigenous cattle and buffalo and enhance the milk yield of crossbred cows by supportive technical, institutional and policy initiatives

Impact of natural resource management interventions on water resources and environmental services in different agroecological regions of India

Natural resource management is critical for addressing issues of water scarcity, land degradation and poor agricultural and livestock productivity especially in rainfed ecologies. This study was conducted in 13 Gram panchayats (cluster of villages) of three states in India representing different agroecological regions. Natural resource management works undertaken through the Mahatma Gandhi National Rural Employment Guarantee Act (MGNREGA) covering water harvesting, pasture land and orchard development were evaluated between November 2015 and January 2016. Density of water harvesting varied from 10 to 250 m3 ha−1 depending on topography and works completed in different villages. These structures have contributed significantly to harvesting surface runoff, enhancing groundwater recharge, reducing soil erosion, and flood control. Irrigation acreage increased between 4 ha and 95 ha which is about 5–10% of the total cultivable area in study villages. In addition, crop productivity increased from 30 to 50% both in rainy and post-rainy seasons compared to non-beneficiary fields. The study showed that the selected natural resource management works have the potential to generate additional economic gain from US$ 60 ha−1 year−1 to US$ 225 ha−1 year−1 along with some indirect benefits to strengthen environmental services and rural livelihood. Overall, the MGNREGA has helped in strengthening ecosystem services, viz., provisioning, regulatory, and supporting services in addition to providing employment opportunities. The study also suggests to follow science-led approach in designing and implementation of various NRM works for further improvements and achieving long term sustainability.

Characterization of Some Vertisols of Different Agro-ecological Regions of India

Vertisols have the capacity to shrink and swell, inducing cracks and distinctive soil structure throughout the soil profile. In India they occur in various agro-ecological regions such as humid tropical (HT), sub-humid moist (SHM), sub-humid dry (SHD), semi-arid moist (SAM), semi-arid dry (SAD) and arid dry (AD) climatic environments and thus indicate an array of soils in a climosequence. In this study benchmark Vertisols, series Panjari is from sub-humid (dry), Kheriis from sub-humid (moist) region, Teligi and Akola series are from semi-arid (dry) and Nimone is from arid agro-ecological region. All the Vertisols in general are characterized by dark colour, angular to sub-angular blocky structure and clay in texture and calcareous. These soils have high bulk density and high water retention capacity. These soils have deep, wide-opened desiccation cracks at the surface which extend deep into the profiles and the depth of cracks increases with aridity. Soils of all climates are dominated by Ca2+ ion in their exchange complex throughout the depth. However, in the sub-humid climate Mg2+ ions tends to dominate in the lower horizon. Whereas the semi-arid dry (Akola) soils have high Na+ ions in their exchange complex. The soils are slightly alkaline to strongly alkaline in nature and poor in organic matter. The CEC varied from 59.3 to 68.2cmol (p+) kg-1 in soil of Panjari series which is highest followed by Teligi series profile. The calcium carbonate (CaCO3) shows gradual increase with depth in all the soil profiles, though it is preferentially accumulated in the sub-surface horizons of lower rainfall region soils.In climosequence the soils of the sub-humid region are generally Typic Haplusterts, soils of semi-arid region are Typic/Sodic/Calcic Haplusterts and soils of arid region are Sodic/ Calcic/ Aridic Haplusterts. The present study demonstrates how the soil properties of Vertisols in a different climate may help in inferring the change in climate in a geologic period.

Assessment of potassium release threshold levels of Vertisols (shrink-swell soils) in different agro-ecological regions of India

Abstract Studies to evaluate the release threshold level of potassium in Vertisols (shrink-swell soils) in different agro-ecological regions of India and how that may help in K fertility interpretations are rare. Thus the objectives of this study were (i) to evaluate the K status of Vertisols through release threshold levels and (ii) to relate the findings with soil K release and fertilizer management. Three established soil series samples were collected from Vertisols of different agro-ecological regions of India. Release threshold level (RTL) was assessed after equilibrating soil with 0.01 M CaCl2 solution having series of soil: solution ratio, after which the remaining amount of exchangeable K was extracted with 1 N NH4OAc (pH 7). Total amount of K extracted (KT = K desorbed in CaCl2 solution plus K extracted with 1 N NH4OAc) remained more or less constant with decreasing K-level up to a certain value (say threshold value) below which KT increased sharply indicating K release from non-labile form. The threshold value in terms of K concentrations (Release Threshold Concentration) of Panjari, Nagpur, Maharashtra (Typic Haplusterts, Sub-humid dry), Teligi, Bellary, Karnataka (Sodic Haplusterts, Semi-arid dry) and Kheri, Jabalpur, Madhya Pradesh (Typic Haplusterts, Sub-humid moist) soils were 0.044–0.049, 0.034–0.062 and 0.043–0.11 mm, respectively. The high release threshold K levels in terms of concentration and activity ratio (AR) were observed in soils of Teligi and Kheri series as compared to soils of Panjari series due to high content of trioctahedral mica in the former two soils. The relationships between clay minerals, release threshold level, exchangeable potassium and non-exchangeable potassium determined by 1 N HNO3 and sodium tetra-phenyl borate (NaBPh4) have been discussed.

Analysis of Multi-location Data of Hybrid Rice Trials Reveals Complex Genotype by Environment Interaction

The All India Coordinated Rice Improvement Project of ICAR-Indian Institute of Rice Research, Hyderabad organizes multi-location testing of elite lines and hybrids to test and identify new rice cultivars for the release of commercial cultivation in India. Data obtained from Initial Hybrid Rice Trials of three years were utilized to understand the genotype × environment interaction (GEI) patterns among the test locations of five different agro-ecological regions of India using GGE and AMMI biplot analysis. The combined analysis of variance and AMMI ANOVA for a yield of rice hybrids were highly significant for GEI. The GGE biplots first two PC explained 54.71%, 51.54% and 59.95% of total G + GEI variation during 2010, 2011 and 2012, respectively, whereas AMMI biplot PC1 and PC2 explained 46.62% in 2010, 36.07% in 2011 and 38.33% in 2012 of the total GEI variation. Crossover interactions, i.e. genotype rank changes across locations were observed. GGE biplot identified hybrids, viz. PAN1919, TNRH193, DRH005, VRH639, 26P29, Signet5051, KPH385, VRH667, NIPH101, SPH497, RH664 Plus and TNRH222 as stable rice hybrids. The discriminative locations identified in different test years were Coimbatore, Maruteru, VNR, Jammu, Raipur, Ludhiana, Karjat and Dabhoi. The AMMI1 biplot identified the adaptable rice hybrids viz., CNRH102, DRH005, NK6303, NK6320, DRRH78, NIPH101, Signet5050, BPH115, Bio452, NPSH2003, and DRRH83. The present study demonstrated that AMMI and GGE biplots analyses were successful in assessing genotype by environment interaction in hybrid rice trials and aided in the identification of stable and adaptable rice hybrids with higher mean and stable yields.

Evaluation of drought using SPEI drought class transitions and log-linear models for different agro-ecological regions of India

Markov chain and 3-dimensional log-linear models were attempted to model drought class transitions derived from the newly developed drought index the Standardized Precipitation Evapotranspiration Index (SPEI) at a 12 month time scale for six major drought prone areas of India. Log-linear modelling approach has been used to investigate differences relative to drought class transitions using SPEI-12 time series derived form 48 yeas monthly rainfall and temperature data. In this study, the probabilities of drought class transition, the mean residence time, the 1, 2 or 3 months ahead prediction of average transition time between drought classes and the drought severity class have been derived. Seasonality of precipitation has been derived for non-homogeneous Markov chains which could be used to explain the effect of the potential retreat of drought. Quasi-association and Quasi-symmetry log-linear models have been fitted to the drought class transitions derived from SPEI-12 time series. The estimates of odds along with their confidence intervals were obtained to explain the progression of drought and estimation of drought class transition probabilities. For initial months as the drought severity increases the calculated odds shows lower value and the odds decreases for the succeeding months. This indicates that the ratio of expected frequencies of occurrence of transition from drought class to the non-drought class decreases as compared to transition to any drought class when the drought severity of the present class increases. From 3-dimensional log-linear model it is clear that during the last 24 years the drought probability has increased for almost all the six regions. The findings from the present study will immensely help to assess the impact of drought on the gross primary production and to develop future contingent planning in similar regions worldwide.

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

Fourteen populations of Meloidogyne graminicola were collected from different agroecological regions of India. Morphological and morphometrical comparisons were made for various nematode life stages. Three populations (Hisar, New Delhi, and Samastipur) were different from typical M. graminicola on the basis of the length of eggs; J2 length, a-value, hyaline tail portion; male length, distance up to excretory pore, spicule and gubernaculum lengths; female length and width, stylet length, distance up to excretory pore, EPST (distance of excretory pore from anterior end / stylet length [females]) ratio, and vulval length. Morphological and morphometrical comparison with closely related species M. graminis, M. oryzae, M. salasi, M. triticoryzae, and M. lini clustered these populations into two groups: Anand, Bhubaneswar, Hyderabad, Jammu, Jorhat, Kalyani, Kanpur, Ludhiana, Mandya, Palampur, Vellayani grouped with M. graminicola, M. triticoryzae and M. salasi; whereas, Hisar, New Delhi, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.

Genetic Variability and Diversity Pattern for Agromorphological Traits in Indian Mustard Germplasm

Genetic diversity studies were carried out on 302 indigenous accessions of Indian mustard [Brassica juncea (L.) Czern & Coss] for variability assessment and identification of trait-specific germplasm. The Indian mustard germplasm was evaluated for eight important morpho-agronomic traits. Geographic information system (GIS), principal component and cluster analysis were carried out to analyse major clusters. Four principal components were identified explaining more than 74% of total variation. Three promising germplasm accessions (IC342781, IC570320 and IC426354) identified with maximum number of seeds per siliqua (>18.0 seeds) were collected from districts Patna (Bihar) and Adilabad (Andhra Pradesh). Similarly, three promising accessions (IC426398, IC521376 and IC395557) were identified with high oil content (>40%) from districts Nizamabad (Andhra Pradesh), Godda (Jharkhand) and Gorakhpur (Uttar Pradesh) respectively. The cluster analysis grouped mainly in two major clusters I and II and further sub-divided into a and b sub-groups. In cluster I sub-group b which indicates a close relationship between traits-the days to mean flowering and days to mean maturity while the plant height was grouped individually. In cluster II, number of primary branches and number of seeds/siliqua showed close relationship with oil content as compared to other traits. GIS based grid maps using Shannon-Weaver diversity index (0–4.0) were generated for showing high variability areas (light red/dark red grids) in different agro-ecological regions of India. The results indicated that Indian mustard germplasm collected by NBPGR from different parts of India has broad genetic base. Thus, promising genotypes identified for various economically important morpho-agronomic traits can be utilized to develop improved varieties of Indian mustard.

In Vitro Propagation and Conservation of Bacopa monnieri L.

Bacopa monnieri L. (common name brahmi) is a traditional and renowned Indian medicinal plant with high commercial value for its memory revitalizer potential. Demand for this herb has further escalated due to popularization of various brahmi-based drugs coupled with reported anticancer property. Insufficient seed availability and problems associated with seed propagation including short seed viability are the major constraints of seed conservation in the gene banks. In vitro clonal propagation, a prerequisite for in vitro conservation by enhanced axillary branching was standardized. We have developed a simple, single step protocol for in vitro establishment, propagation and medium-term conservation of B. monnieri. Single node explants, cultured on Murashige and Skoog's medium supplemented with BA (0.2 mg/L), exhibited shoot proliferation without callus formation. Rooting was achieved on the same medium. The in vitro raised plants were successfully transferred to soil with ~80 % survival. On the same medium, shoots could also be conserved for 12 months with high survival and genetic stability was maintained as revealed by molecular markers. The protocol optimized in the present study has been applied for culture establishment, shoot multiplication and medium-term conservation of several Bacopa germplasm, procured from different agro-ecological regions of India.

Sequence-Related Amplified Polymorphism-PCR Analysis for Genetic Diversity in Rhizoctonia solani Populations Infecting Pulse Crops in Different Agro-Ecological Regions of India

A B S T R A C T Rhizoctonia solani is a destructive fungal pathogen infecting wide range of crop plants including pulses causing wet root rot or web blight disease. The present study was aimed to determine the genetic diversity of R. solani populations using Sequence-Related Amplified Polymorphism (SRAP) markers. The SRAP markers were used for genetic diversity analysis of 89 isolates of R. solani belonging to 7 Anastomosis Groups (AGs) isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India. Out of 30 SRAP primer combinations evaluated, 16 combinations provided amplification with 100% polymorphism and the primer combinations Me1/Em1and Me1/Em4 provided the highest number of bands (14). The isolates of R. solani showed high level of genetic variability and grouped into 7 major clusters at 35% genetic similarity by using unweighted pair group method with an arithmetic average analysis. Bootstrap analysis grouped the isolates into five major clusters at 28% genetic similarity and about 95% isolates shared common sub-grouping patterns in both the analysis. The majority of the isolates representing various AGs were grouped together into different sub-clusters. The molecular clusters did not correspond to agro-ecological regions and crops of the origin of the isolates because of the diversity in the hosts and adopt ability of the pathogen under different environmental conditions prevalent in various parts of the country. First time an attempt was made in the present study to determine the genetic variability of the R. solani populations isolated from different pulse crops representing various AGs using SRAP markers.

Sensitivity Analysis of FAO-56 Penman-Monteith Method for Different Agro-ecological Regions of India

This paper analyzes the sensitivity of reference evapotranspiration (ETo) to climatic variables for different agro-ecological regions of India: semi-arid (Kovilpatti and Parbhani), humid (Mohanpur), and sub-humid (Ludhiana and Ranichauri). The FAO-56 Penman-Monteith (FAO-56 PM) method is used to estimate ETo, and sensitivity of ETo has been studied in terms of change in maximum air temperature (T max ), minimum air temperature (T min ), solar radiation (R s ), average relative humidity (RH avg ), and wind speed (W s ). Sensitivity analysis is performed by increasing and decreasing the climate variables such as T max , T min , R s , and RH avg by one unit of increment and decrement, respectively, up to five units (except for W s ) while keeping the other variables and parameters constant. However, wind speed W s (km h−1) is only increased with an increment of one km h−1 up to five km h−1. The results showed that the change in ETo is linearly related to change in all climate variables (r 2 = 0.97 in most cases) at all sites. Further, ETo is most sensitive to R s at Kovilpatti, Mohanpur and Ranichauri, and to W s at Ludhiana and Parbhani. However, the sensitivity of ETo to the same variable shows considerable variation from site to site and at the same site within the year. ETo is less sensitive to RH avg followed by T min at all sites.

Identification of climate-resilient integrated nutrient management practices for rice-rice cropping system--an empirical approach to uphold food security.

Yield datasets of long-term experiments on integrated nutrient management in rice-rice cropping systems were used to investigate the relationship of variability in rainfall, temperature, and integrated nutrient management (INM) practices in rice-rice cropping system in three different agroecological regions of India. Twelve treatments with different combinations of inorganic (chemical fertilizer) and organic (farmyard manure, green manure, and paddy straw) were compared with farmer's conventional practice. The intraseasonal variations in rice yields are largely driven by rainfall during kharif rice and by temperature during rabi rice. Half of the standard deviation from the average monthly as well as seasonal rainfall during kharif rice and 1 °C increase or decrease from the average maximum and minimum temperature during rabi rice has been taken as the classification of yield groups. The trends in the date of effective onset of monsoon indicate a 36-day delay during the 30-year period at Rajendranagar, which is statistically significant at 95 % confidence level. The mean annual maximum temperature shows an increasing trend in all the study sites. The length of monsoon also showed a shrinking trend in the rate of 40 days during the 30-year study period at Rajendranagar representing a semiarid region. At Bhubaneshwar, the application of 50 % recommended NPK through chemical fertilizers and 50 % N through green manure resulted in an overall average higher increase of 5.1 % in system productivity under both excess and deficit rainfall years and also during the years having seasonal mean maximum temperature ≥35 °C. However, at Jorhat, the application of 50 % recommended NPK through chemical fertilizers and 50 % N through straw resulted in an overall average higher increase of 7.4 % in system productivity, while at Rajendranagar, the application of 75 % NPK through chemical fertilizers and 25 % N through green manusre resulted in an overall average higher increase of 8.8 % in system productivity. This study highlights the adaptive capacity of different integrated nutrient management practices to rainfall and temperature variability under a rice-rice cropping system in humid, subhumid, and semiarid ecosystems.

Diversity of Rhizoctonia solani associated with pulse crops in different agro-ecological regions of India

Four hundred seventy Rhizoctonia solani isolates from different leguminous hosts originating from 16 agro-ecological regions of India covering 21 states and 72 districts were collected. The disease incidence caused by R. solani varied from 6.8 to 22.2% in the areas surveyed. Deccan plateau and central highlands, hot sub-humid ecoregion followed by northern plain and central highlands and hot semi-arid ecoregion showed the highest disease incidence. R. solani isolates were highly variable in growth diameter, number, size and pattern of sclerotia formation as well as hyphal width. The isolates obtained from aerial part of the infected plants showing web blight symptoms produced sclerotia of 1-2mm in size whereas, the isolates obtained from infected root of the plants showing wet root rot symptoms produced microsclerotia (<1mm). Majority of R. solani isolates showed <8μm hyphal diameter. Based on morphological characters the isolates were categorized into 49 groups. Seven anastomosis groups (AGs) were identified among the populations of R. solani associated with the pulse crops. The frequency (25.6%) of AG3 was the highest followed by AG2-3 (20.9%) and AG5 (17.4%). The cropping sequence of rice/sorghum/wheat-chickpea/mungbean/urdbean/cowpea/ricebean influenced the dominance of AG1 (16.3%). Phylogenetic analysis utilizing ITS-5.8S rDNA gene sequences indicated high level of genetic similarity among isolates representing different AGs, crops and regions. ITS groups did not correspond to the morphological characters. The sequence data from this article has been deposited with NCBI data libraries with JF701707 to JF701795 accession numbers.

Molecular detection and genotyping of Fusarium oxysporum f. sp. psidii isolates from different agro-ecological regions of India

Twenty one isolates of Fusarium oxysporum f. sp. psidii (Fop), causing a vascular wilt in guava (Psidium guajava L.), were collected from different agro-ecological regions of India. The pathogenicity test was performed in guava seedlings, where the Fop isolates were found to be highly pathogenic. All 21 isolates were confirmed as F. oxysporum f. sp. psidii by a newly developed, species-specific primer against the conserved regions of 28S rDNA and the intergenic spacer region. RAPD and PCR-RFLP were used for genotyping the isolates to determine their genetic relationships. Fifteen RAPD primers were tested, of which five primers produced prominent, polymorphic, and reproducible bands. RAPD yielded an average of 6.5 polymorphic bands per primer, with the amplified DNA fragments ranging from 200-2,000 bp in size. A dendrogram constructed from these data indicated a 22-74% level of homology. In RFLP analysis, two major bands (350 and 220 bp) were commonly present in all isolates of F. oxysporum. These findings provide new insight for rapid, specific, and sensitive disease diagnosis. However, genotyping could be useful in strain-level discrimination of isolates from different agro-ecological regions of India.

Morphological variability in cultivated and wild species of Luffa (Cucurbitaceae) from India

Immature fruits of cultivated species of Luffa are commonly used as summer vegetable in India. Rich morphological variability occurs in cultivated species of Luffa in different growing regions of the country. To study morphological variability in cultivated and wild Luffa from different agro-ecological regions of India, a total of seventy accessions were assembled. Morphological variations in leaf, fruit and seed characters were found to delimit cultivated and wild Luffa species. Cluster analysis revealed two major clusters, one comprising of 36 accessions of Luffa acutangula and the other of 30 accessions belonging to L. aegyptiaca. A local cultivar “Satputia” (L. hermaphrodita) was classified along with cultivated L. acutangula and wild L. acutangula var. amara. Wild species namely L. graveolens and L. echinata were clustered closer to the L. acutangula.

RETRACTED ARTICLE: Length–weight relationship and condition factor of nine possible crosses of three stocks of giant freshwater prawn, Macrobrachium rosenbergii from different agro-ecological regions of India

RETRACTED ARTICLE: Length–weight relationship and condition factor of nine possible crosses of three stocks of giant freshwater prawn, Macrobrachium rosenbergii from different agro-ecological regions of India

Molecular diversity analysis of Rhizoctonia solani isolates infecting various pulse crops in different agro-ecological regions of India

Genetic diversity of 89 isolates of Rhizoctonia solani isolated from different pulse crops representing 21 states from 16 agro-ecological regions of India, 49 morphological, and 7 anastomosis groups (AGs) was analyzed using 12 universal rice primers (URPs), 22 random amplified polymorphic DNA (RAPD), and 23 inter-simple sequence repeats (ISSR) markers. Both URPs and RAPD markers provided 100% polymorphism with the bands ranging from 0.1 to 5kb in size, whereas ISSR markers gave 99.7% polymorphism with the bands sizes ranging from 0.1 to 3kb. The marker URP 38F followed by URP13R, URP25F, and URP30F, RAPD marker R1 followed by OPM6, A3 and OPA12 and ISSR3 followed by ISSR1, ISSR4, and ISSR20 produced the highest number of amplicons. R. solani isolates showed a high level of genetic diversity. Unweighted pair group method with an arithmetic average (UPGMA) analysis grouped the isolates into 7 major clusters at 35% genetic similarity using the three sets of markers evaluated. In spite of using three different types of markers, about 95% isolates shared common grouping patterns. The majority of the isolates representing various AGs were grouped together into different sub-clusters using all three types of markers. Molecular groups of the isolates did not correspond to agro-ecological regions or states and crops of the origin. An attempt was made for the first time in the present study to determine the genetic diversity of R. solani populations isolated from different pulse crops representing various AGs and agro-ecological regions.

Genetic diversity analysis and development of SCAR marker for detection of Indian populations of Fusarium oxysporum f. sp. ciceris causing chickpea wilt

Genetic diversity of the isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt collected from 12 states representing different agro-ecological regions of India was determined through randomly amplified polymorphic DNA (RAPD) markers. The UPGMA cluster analysis grouped the isolates into eight categories showing high magnitude of genetic diversity. Each group had the isolates from different states present in various agro-ecological regions of India. Therefore, the groups generated through the RAPD analysis were not corresponding to area of the origin of the isolates. The RAPD primers, namely, OPA 7 and OPA 11 produced Foc specific fragment of ≈1.3 kb and ≈1.4 kb, respectively in all the isolates. These fragments were eluted, purified, cloned in pGEM-T Easy vector and sequenced. Primers were designed with sequence information of these two fragments using primer.3 software. Two sets of sequence characterized amplified region markers (SC-FOC 1 and SC-FOC 2) developed from the sequences of these fragments were found to be specific to Foc and produced an amplicon of 1.3 and 1.4 kb, respectively. These set of markers were validated against the isolates of the pathogen collected from different locations of India representing various races of the pathogen. They are non-specific to the other Fusarium species, Rhizoctonia solani and R. bataticola.