Difference In Mean Platelet Volume Research Articles

Separation of platelets by size in a microfluidic device based on controlled incremental filtration.

The significant biological and functional differences between small and large platelets suggested by recent studies could have profound implications for transfusion medicine. However, investigating the relationship between platelet size and function is challenging because separating platelets by size without affecting their properties is difficult. A standard approach is centrifugation, but it inevitably leads to premature activation and aggregation of separated platelets. This paper describes the development and validation of a microfluidic device based on controlled incremental filtration (CIF) for separating platelets by size without the cell damage and usability limitations associated with centrifugation. Platelet samples derived from whole blood were used to evaluate the dependence of the CIF device separation performance on design parameters and flow rate, and to compare the properties of PLT fractions generated by the CIF device with those produced using a centrifugation protocol in a split-sample study. This was accomplished by quantifying the platelet size distribution, mean platelet volume (MPV), platelet-large cell ratio (P-LCR) and platelet activation before and after processing for all input and output samples. The 'large platelet' fractions produced by the CIF device and the centrifugation protocol were essentially equivalent (no significant difference in MPV and P-LCR). Platelets in the 'small platelet' fraction produced by the CIF device were significantly smaller than those produced by centrifugation (lower MPV and P-LCR). This was because the CIF 'small platelet' fraction was contaminated by much fewer large platelets (∼2-times lower recovery of >12 fL platelets) and retained the smallest platelets that were discarded by the centrifugation protocol. There was no significant difference in platelet activation between the two methods. However, centrifugation required a substantial amount of additional anticoagulant to prevent platelet aggregation during pelleting. Unlike centrifugation, the CIF device offered continuous, flow-through, single-step processing that did not cause platelet aggregation. Such a capability has the potential to accelerate the basic studies of the relationship between platelet size and function, and ultimately improve transfusion practice, particularly in the pediatric setting, where the need for low-volume, high-quality platelet transfusions is most urgent.

Validity of the Mean Platelet Volume and Revised Clinical Risk Index for Babies (CRIB-II) Score to Assess Mortality Risk in Preterm Infants

Objective: This study aimed to investigate the validity of the mean platelet volume (MPV) alone and MPV combined with the revised Clinical Risk Index for Babies (CRIB II) score to predict neonatal mortality in preterm infants. Materials and Methods: This retrospective observational study performed between May 2018 and June 2021 included preterm neonates (gestational age 23–32 weeks) admitted to a tertiary neonatal intensive care unit (NICU) within 12 hours of birth, who were followed up until death or discharge. MPV was recorded at admission and within 72 hours before death or discharge. The CRIB II score variables were assessed within 12 hours of birth, and the area under the receiver operating characteristic curve (AUC) was calculated for the MPV and CRIB II scores to predict neonatal mortality. Results: We investigated 404 newborns, of whom 28 (6.9%) died. The mean neonatal gestational age was 28.6±2.8 weeks. The MPV was significantly higher in non-survivors than in survivors (p=0.001). The AUC for the MPV and the CRIB II score was 0.68 (95% CI: 0.55–0.80) and 0.85 (95% CI: 0.79–0.91), respectively. The AUC for the MPV combined with the CRIB II score was 0.91 (95% CI 0.87–0.95). The difference in MPV at birth between survivors and non-survivors was statistically non-significant. Conclusion: MPV was found to be a poorer predictor of neonatal mortality than the CRIB II score; however, MPV combined with the CRIB II score demonstrated significantly improved predictive ability for preterm infant mortality risk.

The bleeding diathesis in patients with hereditary haemorrhagic telangiectasia is not due to impaired platelet function

BackgroundPatients with the rare disease; Hereditary haemorrhagic telangiectasia (HHT) often bleed from telangiectatic lesions in mucosal surfaces. Studies suggest that impaired platelet function may also play a role in their bleeding tendency. The aim of the present study was to investigate whether HHT-patients with epistaxis have impaired platelet function.MethodWe conducted a case–control study based on a sample size calculation and included 22 HHT-patients (inclusion criteria: epistaxis severity score ≥ 4, no intake of medicine affecting platelet function the last 5 days, HHT-type 1 or 2, age ≥ 18 years) and 20 controls. We assessed the platelet function with standard haemostasis parameters, flow cytometry (platelet function and micro aggregation), rotational thromboelastometry and Platelet Function Analyzer 200.ResultsWe found no significant difference in mean platelet volume and immature platelet fraction and no difference in platelet activation as measured by exposure of CD62P, CD63P and PAC1 binding. Nor did we find a significant difference in platelet aggregation response in HHT-patients compared with the control group for all agonists (thrombin receptor activating peptide, adenosine diphosphate and collagen-related peptide). The PFA-200 analysis was without difference between the two groups and thromboelastometry showed no impairment of global haemostasis.ConclusionReduced platelet function is unlikely to contribute to the frequent and long bleeding episodes that HHT-patients suffer from. We propose that further studies should focus on whether patients with HHT have hypercoagulability.

Role of neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio as potential biomarkers in Behçet's disease: ameta-analysis.

The mean platelet volume (MPV), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) have attracted interest as possible indicators of inflammation and disease activity in various diseases. This meta-analysis assessed the association between NLR, MPV, PLR, and Behçet's disease (BD) and their correlation with disease activity and thrombosis. Athorough search of the Medline, Embase, and Cochrane databases was performed to identify relevant studies. Studies comparing NLR, MPV, and PLR between patients with BD and healthy controls, as well as studies examining these measures in connection with disease activity and thrombosis in BD satisfied the inclusion criteria. The standardized mean difference (SMD) and 95% confidence interval (CI) were used to calculate the effect sizes. This meta-analysis included 24articles. The findings revealed no discernible differences in MPV between the BD and control groups (p = 0.992). NLR was substantially higher in the BD group than in the control group (p < 0.001). PLR was higher in the BD group than in the control group (p = 0.030), indicating that BD is associated with alarger PLR. Patients with active and inactive BD did not vary significantly in terms of disease activity according to the MPV. Comparing MPV between patients with BD with and without thrombosis showed no discernible changes. However, individuals with active BD had aconsiderably higher NLR and PLR than those with inactive BD (p = 0.003 and p = 0.005, respectively). The statistical significance threshold for the association between NLR, PLR, and thrombosis in patients with BD was not met. NLR and PLR can be regarded as general markers of inflammation according to the results of this meta-analysis.

Effect of Platelet Parameters on Linezolid-Related Thrombocytopenia in Hospitalized Patients.

Linezolid-induced thrombocytopenia incidence varies considerably. Linezolid-related thrombocytopenia in patients has received few studies which have investigated risk factors including platelet parameters except for platelet counts. The study aims to analyze the effect of platelet parameters, including mean platelet volume and platelet large cell ratio, on linezolid-related thrombocytopenia in patients. The effect of platelet parameters on linezolid-related thrombocytopenia was identified by univariate and multivariate logistic regressions. A Kaplan-Meier survival analysis was carried out to compare the survival of patients who developed linezolid-related thrombocytopenia with patients who did not. Thrombocytopenia occurred at a rate of 41.5% (66/159) after linezolid therapy in hospitalized patients. Platelet parameters, including the difference in mean platelet volume (MPV/fL=0.08 (-1.2-0.9)vs-0.5 (-1.5-0.3), (OR, 0.459; P = 0.001), the difference in platelet large cell ratio (PLCR/fL=0.9 (-5.1-6.2)vs-3.8 (-8.6-2.4), (OR, 1.156; P = 0.001), baseline platelet counts (OR, 0.995; P = 0.006) and duration of linezolid therapy≥10d (OR, 1.346; P = 0.007), were significantly associated with linezolid-related thrombocytopenia in hospitalized patients. In addition, other risk factors which also are associated with linezolid-related thrombocytopenia include baseline red blood cells, co-medication with parecoxib and co-medication with caspofungin. Accumulated in-hospital mortality of patients with thrombocytopenia was significantly higher than that of patients without thrombocytopenia during linezolid treatment (19.7% vs 8.6%, P = 0.003). The difference in mean platelet volume, the difference in large platelet ratio, baseline platelet counts and duration of linezolid therapy≥10d significantly affected the development of linezolid-related thrombocytopenia in hospitalized patients.

Mean platelet volume and neutrophil/lymphocyte ratio in adolescent idiopathic scoliosis: can they be predictive value in diagnosis?

In our study, we evaluated whether mean platelet volume (MPV) and neutrophil lymphocyte ratio (NLR) are predictive values in the diagnosis of Adolescent Idiopathic Scoliosis in patients diagnosed with scoliosis in our clinic. Approximately 15000 patients who applied to our spine outpatient clinic with the suspicion of scoliosis between 2011 and 2018 were reviewed retrospectively. 292 patients were included in the study. The patients were divided into 3 groups. Group 1; control group group 2; group with the possibility of developing scoliosis under follow-upand group 3; the patient group diagnosed with scoliosis. Spinal curvature degrees of the patients were measured using the Cobb method. The MPV and NLR values of the patients were compared with the degree of curvature measured by the cobb method. NLR was 2.17 ± 2.10 K/ul in Group 1, 2.42 ± 1.76 K/ul in Group 2, and 2.72 ± 3.91 K/ul in Group 3. Although the NLR of the 3rd group was higher than the other 2 groups, it was not statistically significant. (p &gt; 0.05). MPV was 7.90 ± 1.07 fL in Group 1, 7.95 ±1.39 fL in Group 2, 8.33 ± 1.37 fL in Group 3.MPV was higher in Group 3 and was found to be statistically significant (p=0.024). After adjusting for the effects of gender and age variables on the groups, the difference in MPV between groups became more significant (p=0.017) . While there was no statistically significant difference between the groups in terms of NLR, it was observed that MPV was statistically significantly higher in patients with AIS.Could this relationship be a promising inflammatory marker for AIS? We think that this question should be answered by studies involving larger patient and control groups.

Akut Pankreatitte Yeni Bir İnflamatuar Belirteç Olarak Ortalama Trombosit Hacmi ve C-Reaktif Protein ile Başvuru Anındaki Ranson Skoru ile İlişkisi

Aim: The study’s aim is to evaluate mean platelet volume (MPV) as a marker of inflammation in patients&#x0D; with acute pancreatitis (AP) and to analyse the relationships among MPV, CRP (C-reactive protein) and&#x0D; Ranson’s score.&#x0D; Material and Methods: In this study, 119 patients with AP (mean age 53.8 ± 18.0 years) and 88 healthy&#x0D; control group (mean age 53.1 ± 6.8 years) were enrolled. Among the patients with AP, 75 were classified&#x0D; as having biliary AP, and 44 were classified as having nonbiliary AP. All patients’ demographic data,&#x0D; clinical and laboratory findings and Ranson’s scores were examined from the hospital’s database.&#x0D; Results: MPV was significantly lower among patients with AP than among the control group (p = 0.001).&#x0D; CRP was significantly higher among patients with AP than among the control group (p&lt; 0.001). The&#x0D; difference in MPV between the biliary and nonbiliary AP group was statistically not significant. When&#x0D; we compared MPV based on patients’ lengths of hospital stay, there was no significant difference. In&#x0D; correlation analysis, there was no correlation among CRP, Ranson’s score and serum MPV levels.&#x0D; Conclusion: We observed that MPV levels in the AP group were lower than healthy controls. Thus, like&#x0D; other inflammation markers, MPV might be a useful marker for AP diagnosis.

Relationship between platelet activation markers and spontaneous abortion: A meta-analysis.

Numerous studies have indicated that mean platelet volume (MPV) and platelet distribution width (PDW) were correlated with spontaneous abortion (SAB), but the results were contradictory. Hence, we performed a meta-analysis to assess the association of MPV and PDW with SAB. We systematically searched on China National Knowledge Internet, PubMed, and Embase up to May 2022 to retrieve eligible studies. The synthesized standard mean difference (SMD) with a 95% confidence interval (CI) was used to evaluate the correlation of MPV and PDW with SAB. A total of 20 studies were finally included in this meta-analysis. The pooled analysis results showed that PDW was markedly increased in patients with SAB versus women with a healthy pregnancy (SMD = 1.03; 95% CI: 0.51-1.54; p = 0.0001), while there were no significant differences in MPV between women with SAB and those with healthy pregnancy (SMD = 0.19; 95% CI: -0.26 to 0.65; p = 0.40). Therefore, PDW may serve as a potential marker for predicting SAB. However, homogeneous and multiethnic studies with larger sample sizes are warranted to validate our findings due to several limitations in this meta-analysis.

A Study of Platelet Count and Mean Platelet Volume in Newborn of Preeclamptic Mother

Introduction: Pregnancy is a physiological process. Preeclampsia is the commonest complication during pregnancy. This condition might severely affect the health of mothers and their newborns. Newborn of mothers with preeclampsia are more liable for intrauterine growth retardation and may be delivered prematurely. Aim of the Study: The aim of this study was to evaluate the platelet count and mean platelet volume in newborn of preeclamptic mother. Methods: This cross-sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka, Bangladesh from January 2017 to December 2017. Total 60 newborns were included in this study. The subjects were divided into 2 groups. Group A (Study group): Thirty (30) newborns of preeclamptic mother and Group B (Control group): Thirty (30) newborns of healthy pregnant mother. The subjects were selected from Department of Obstetrics and Gynecology, Dhaka Medical College Hospital, Dhaka on the basis of exclusion and inclusion criteria. Five (5) ml. of cord blood was collected from each newborn after delivery and was analyzed for Platelet count and mean platelet volume. These parameters were estimated in the Department of Hematology, Dhaka Medical College Hospital, Dhaka. Data were collected in pre- designed structured questionnaire form by the researcher herself. For statistical analysis Unpaired Student’s “t” test, Chi Square test and Pearson’s correlation coefficient (r) test were performed as applicable using SPSS for windows version 16.0. p value &lt;0.05 was accepted as level of significance. Results: The mean (± SD) platelet count was 164.77 ± 79.44 × 103/µl and 212.83 ± 54.04 ×103/µl in group A and B respectively. In this study, the mean (±SD) platelet count was lower in group A in comparison to that of group B which was statistically significant (p&lt;0.001). The mean (± SD) platelet volume was 8.90±1.15 fl and 8.30±1.45 fl in group A and B respectively. In this study, the mean (±SD) platelet volume was higher in group A in comparison to that of group B which was not statistically significant. Maternal systolic blood pressure showed negative correlation (- 0.952) with platelet count of newborn in preeclamptic mother, which was statistically significant (p&lt;0.001). Maternal diastolic blood pressure showed negative correlation (- 0.960) with platelet count of newborn in preeclamptic mother, which was statistically significant (p&lt;0.001). Maternal systolic blood pressure showed negative correlation (-0.973) with mean platelet volume of newborn in preeclamptic mother, which was not statistically significant (p&gt;0.05). Maternal diastolic blood pressure showed negative correlation (-0.964) with mean platelet volume of newborn in preeclamptic mother, which was not statistically significant (p&gt;0.05). Conclusion: From the findings of the study, it can be concluded that the platelet count of newborn of preeclamptic mother was significantly lower in comparison to newborn of healthy pregnant mother but they were within normal range. This difference was significantly related to severity of maternal blood pressure. But there was no statistically significant difference in mean platelet volume between the groups. There was no statistically significant relation of mean platelet volume to maternal blood pressure.

A mean platelet volume in inflammatory bowel disease: A systematic review and meta-analysis.

BackgroundInflammatory bowel disease (IBD) is a chronic gastrointestinal tract inflammatory state, which is affecting millions of individuals in the world. It can affect alimentary canals such as colon, rectum, ileum and other parts. In IBD, platelet parameters underwent several changes. Therefore, the aim of this review was determining the estimated pooled mean platelet volume and mean difference in inflammatory bowel disease to elucidate its potential diagnostic value.MethodsArticles were extensively searched in bibliographic databases using Medical Subject Heading and entry phrases or terms. In addition, articles were directly searched in Google Scholar to account for the studies omission in searching bibliographic databases. Observational (cohort, cross-sectional and case-control) studies, published in English language and conducted on IBD were included. For studies meeting the eligibility criteria, the first author’s name, publication year, population, study design, study area, sample size, mean platelet volume and standard deviation were extracted and entered in to Microsoft-excel. The analysis was done by Stata version 11. In order to estimate the pooled mean platelet volume and mean difference, random effect model was done. The heterogeneity was quantified using Higgin’s I2 statistics. Publication bias was determined using Egger’s test statistics and funnel plot. Sub-group analysis based on population carried to reduce heterogeneity.ResultsA total of 17 relevant articles with 2957 participants (1823 IBD cases and 1134 healthy controls) were included to this study. The pooled estimated MPV was 9.29fl; 95% CI: 9.01–9.57 and 9.50fl; 95% CI: 8.81–10.20 in IBD and control groups, respectively. The standardized pooled estimate of mean difference in mean platelet volume was -0.83fl; 95% CI: -1.15, -0.51; I2: 93.1%; P-value < 0.001. In subgroup analysis based on population, the highest estimated mean difference in MPV was observed among patients of CD; -2.30; 95% CI: -3.46, -1.14; I2: 97.8%; P-value < 0.001.ConclusionAccording to the current systematic review and meta-analysis, mean platelet volume was lower in IBD compared to control. The decreased mean platelet volume could be attributed to platelet consumption or sequestration associated with the progression of IBD. As a result, in IBD, mean platelet volume can provide diagnostic and prognostic information.

Evaluation of hematological parameters in inactive hepatitis B infection; neutrophil to lymphocyte ratio and mean platelet volume

AIM: It is estimated that the hepatitis B virus, a serious global public health problem, affects more than 250 million people. Herein, our objective was to quantify several hemogram measurements, like neutrophil-lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and mean platelet volume (MPV) and to determine their relationship with inflammation in hepatitis B infection (HBV).&#x0D; MATERIAL AND METHOD: This study included 94 patients aged 218 years, who were under follow-up at our hospital for hepatitis B infection between January 1st, 2005 and December 31st, 2015. These individuals served as the patient group. The control group was composed of 88 healthy children of similar age and sex.&#x0D; RESULTS: There was no significant difference concerning neutrophil count and NLR (p=0.698 and 0.842, respectively). A significantly lower absolute lymphocyte count significantly and a significantly higher MPV was found in the hepatitis B-positive group than in the controls (p 0.05 for both comparisons). The groups had similar hematocrit, hemoglobin, platelet count, RBC count, and white blood cell (WBC) count (p=0.642, p=0.388, p=0.418, p=0.366, and p=0.824, respectively). The two groups individually showed a positive correlation between platelet count and MPV (r=0.32, p=0.01 and r=0.36, p=0.01, respectively). NLR and MPV were positively correlated in both groups (r=0.38, p=0.01 and r=0.41, p=0.01, respectively).&#x0D; CONCLUSION: In conclusion, the study groups did not exhibit any significant difference concerning NLR, PLR, and PLT/MPV levels but it found a significant intergroup difference in MPV. We believe it is of great importance to develop simpler, inexpensive, and easy-to-use noninvasive methods to determine the level of inflammation in chronic hepatitis B infection; Parameters, such as NLR, PLR, and MPV, can be simple and rapid test markers to serve these purposes.

Mean Platelet Volume in the Diagnosis of Acute Appendicitis in the Pediatric Population: A Systematic Review and Meta-Analysis.

Background: Mean Platelet Volume (MPV) has been suggested as a biomarker for acute appendicitis (AA) in the adult population. The utility of MPV in pediatric AA remains vague. This systematic review and meta-analysis aimed to systematically summarize and compare all relevant data on MPV as a diagnostic biomarker for AA in children. Methods: Databases were systematically searched using keywords ((mean platelet volume) OR mpv) AND (appendicitis). The inclusion criteria were all comparative studies of MPV in children aged less than 18 years and diagnosed with AA. Two authors independently assessed the methodological quality using the Downs and Black scale. Results: We included fourteen studies in the final meta-analysis; most were retrospective. Eight studies compared the MPV values between AA and non-AA; four studies compared the same between AA and healthy controls; two studies compared the MPV values among all three groups. The estimated heterogeneity among the studies for all outcomes was high and statistically significant. The pooling the data showed no statistically significant difference in MPV (weighted mean difference (WMD) = −0.42, 95% CI = (−1.04, 0.20), p = 0.19) between AA and healthy controls or AA and non-AA cases (WMD = 0.01, 95% CI = (−0.15, 0.17), p = 0.90). Conclusion: While MPV levels seem to have some utility in adult AA diagnosis, MPV levels should not dictate treatment options in pediatric AA.

Relationship Between Retinopathy and Mean Platelet Volume

OBJECTIVE: Platelet activation is thought to play a role in the angiogenesis process involved in the pathophysiology of retinopathy of prematurity (ROP). We planned this study to investigate whether the mean platelet volume (MPV), used to assess platelet activation, could be used as a biomarker in the diagnosis and treatment of ROP. STUDY DESIGN: In our study, we evaluated infants who underwent ROP examination among infants born at 32 weeks or less and/or 1500 grams or less as well as infants who had more than these values but experienced a bad neonatal period. These patients were divided into two groups as those with and without ROP, and in those with ROP group, as requiring treatment and not requiring treatment. We recorded the patients’ identity, maternal characteristics, antenatal/natal/postnatal features, and complications during follow-up, ROP control times, and complete blood count parameters (platelet count, MPV, platelet count/MPV). Primarily, we evaluated the differences of platelet parameters, especially the MPV values, between treatment requiring ROP and non-treatment requiring ROP groups and secondarily, we evaluated the correlation between scanned parameters and ROP development. RESULTS: Of the 144 patients included in the study, 49 patients (34%) had ROP and 25 patients (16.6%) had ROP requiring treatment. There were statistically significant differences between patients who were diagnosed with ROP and who were not, in terms of gestational age, birth weight, respiratory distress syndrome, surfactant use, duration of oxygen use, intraventricular hemorrhage, patent ductus arteriosus, neonatal sepsis, number of blood transfusions, necrotizing enterocolitis, bronchopulmonary dysplasia and time to catch up birth weight. However, there was no significant difference in terms of MPV, platelet count and platelet/MPV ratio. The gestational week, invasive ventilation day and duration of oxygen use, BPD and time to catch up birth weight were found to be statistically high in treatment-requiring rather than non-treatment-requiring ROP groups. There was no significant difference in MPV, platelet count, platelet/MPV ratio among these groups. A statistically significant difference was found in the most advanced stage ROP and plus existence in the treatment requiring ROP group. CONCLUSION: As a result of our study, we could not find enough data to say that MPV is a marker that can be used in the diagnosis of ROP and in measuring the severity of retinopathy. We suggest that more studies should be done on this subject.

Characteristics of platelet-associated parameters and their predictive values in Chinese patients with affective disorders

ObjectivePlatelets are increasingly considered to play an important role in inflammation and are being regarded as a putative bridge linking mental diseases and inflammatory response. Platelet-associated haematological parameters including mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), systemic immune-inflammation index (SII), platelet to lymphocyte ratio (PLR), platelet to albumin ratio (PAR) and red blood cell distribution width (RDW) to platelet ratio (RPR), have been recently investigated as simple, easily available, and inexpensive inflammatory markers. In this study, we aimed is to use large-scale clinical data to study platelet parameters in patients with affective disorders, to further investigate the predictive power of platelet parameters for major depressive disorder (MDD) and bipolar disorder (BD).MethodsThe retrospective, naturalistic, cross-sectional study analysed the data of 14,007 Chinese affective disorder patients, including 4,801 patients with first-episode MDD, 4,098 patients with recurrent MDD, 3,444 patients with BD manic episodes and 1,664 patients with BD depressive episodes. Meanwhile, 6,847 healthy subjects were served as the control group. The differences in the MPV, PDW, PCT, SII, PLR, PAR, RPR and albumin among different groups were compared, and the contributing factors for the occurrence of MDD or BD were analysed.ResultsThere were significant differences in MPV, PDW, PCT, SII, PLR, RPR and albumin values among the study groups. In the subjects, patients experiencing BD manic episodes had the highest mean values of MPV and SII, patients experiencing BD depressive episodes had the lowest mean values of platelet counts and PAR, and patients with MDD had the highest mean values of PLR and RDW. The levels of MPV, PDW and albumin were independently correlated with MDD and BD, and they are important predictors for differentiating patients with MDD or BD from healthy controls.ConclusionsOur study demonstrated that different affective disorders have unique platelet parameter variation patterns, highlighting the role of platelet parameters and systemic inflammation in the pathophysiology of MDD and BD.

The Performance of Hematological Parameters for Diagnosis of Acute Appendicitis in Children

Early diagnosis of pediatric appendicitis is still difficult to make and 30-40% of cases do not show typical classic symptoms. The negative appendectomy rate and the risk of perforation reach 20% so that new, easy, inexpensive and accurate additional examination is needed. This study aimed to compare platelet distribution width (PDW) and mean platelet volume (MPV) values of appendicitis and control patients. This study is retrospective case control study. Differences in MPV and PDW levels in patients with appendicitis and controls were analyzed with a t-test and determination of sensitivity, specificity and accuracy of the two markers in appendicitis diagnosis were performed by ROC analysis The mean MPV appendicitis was significantly lower (p &lt;0.05) compared to controls (8.3 ± 1.9; 9.2 ± 1.5) while PDW of appendicitis patients did not differ significantly from controls (10.4 ± 1, 4, 10.7 ± 2.4; p&gt; 0.05). AUC MPV as a diagnostic tool for appendicitis 0.619 with a normal limit of 6.6 fl has a sensitivity of 90% and specificity of 30%. MPV can be used for the diagnosis of appendicitis with a normal limit of 6.6 fl having a sensitivity of 90% and specificity of 30%. There was no significant difference between PDW of appendicitis patients with controls. There was no significant difference between MPV levels of patients with simple appendicitis and complications.&#x0D; Keywords: Appendicitis, MPV, PDW, Platelet

Changing profile of platelet activity and turnover indices during treatment response of immune thrombocytopenia.

Both platelet count and function change after treatment of immune thrombocytopenia. Platelet function can be measured by plasma markers, including platelet activity [e.g., soluble P-selectin (sP-selectin) and soluble CD40 ligand (sCD40L)] and platelet turnover markers [e.g., glycocalicin (GC)]. Patients were classified into no response (NR, including new diagnosis), partial response (PR) and complete response (CR). One hundred and sixteen samples (29 CR, 32 PR, 55 NR) from 79 patients were collected. Plasma markers (sP-selectin, sCD40L and GC) were measured by ELISA. Platelet counts and mean platelet volume (MPV) were obtained in the clinical laboratory using GenS System-2. The results showed that responsive patients (PR + CR) had higher levels of sP-selectin (P = 0.026) and sCD40L (P = 0.001). Although there was no difference in MPV (P = 0.077) or GC (P = 0.078), there was a marked decrease of GC index (P < 0.001) in responsive patients. Paired sample analysis showed no difference in sP-selectin, sCD40L, MPV or GC but significant difference in GC index (P = 0.017) between NR and PR. Another paired sample analysis showed no difference in sP-selectin, sCD40L, MPV or GC but significant difference in GC index (P = 0.029) between PR and CR. Patients with refractory and newly diagnosed disease had a significant difference in GC (P = 0.020) and sCD40L (P = 0.001), despite similarly low platelet counts. In conclusion, platelet activity markers (sP-selectin and sCD40L) and GC indices change in parallel with treatment response. Plasma levels of GC and sCD40L may be predictors of treatment response.

Vitamin D Level in Patients with Consecutive Acute Coronary Syndrome Is Not Correlated with the Parameters of Platelet Activity.

Coronary artery disease continues to be the leading cause of death in developed countries. Elevated mean platelet volume (MPV) is associated with an increased incidence of myocardial infarction (MI) and MI-related mortality. Vitamin D concentrations affect the level and function of platelets, which are the crucial mediator of atherothrombosis and plaque rupture. The main aim of this study was to examine the relationship of serum 25-hydroxyvitamin D (25(OH)D) levels with the platelet activity in patients with a history of an acute coronary syndrome (ACS). This prospective study recruited 268 patients with a history of MI who underwent coronary angiography due to the suspicion of another ACS. Serum 25(OH)D concentration was determined by electrochemiluminescence. Platelet activity was assessed using the MPV and platelet-large cell ratio (P-LCR) parameters. There was no significant difference in MPV and P-LCR values between patients diagnosed with subsequent MI and patients with chronic coronary syndrome (CCS). A significantly lower level of 25(OH)D was demonstrated in patients who had another MI compared to those with CCS (p < 0.05). No significant correlation of 25(OH)D concentrations with platelet activity parameters values was found. The subgroup of patients with consecutive MI was characterized by significantly lower serum vitamin D levels, but this was not related to the analyzed parameters of platelet activity.

The relation between mean platelet volume and platelet distribution width to COVID-19 severity

Background: Wide spectrum of clinical manifestation and severity of COVID-19 led to further studies to find a simple biomarker used to predict the severity of COVID-19. We investigated the role of mean platelet volume (MPV) and platelet distribution width (PDW), widely available parameters, as predictor of COVID-19 severity.Methods: We conducted a single center analytic observational study to evaluate the relationship between MPV and PDW values and COVID-19 severities. A total of 123 subjects of COVID-19 within October to December 2020 included in the analysis.Results: Analysis showed a statistically significant difference in MPV, PDW, and D-dimer between COVID-19 severities (p&lt;0.001, p=0.002, and p&lt;0.001). Correlation coefficient between MPV, PDW, and D-dimer with COVID-19 severity were 0.28 (p=0.002); 0.22 (p&lt;0.001), and 0.81 (p&lt;0.001), but there’s no correlation between MPV (p=0.176) and PDW (p=0.383) with D-dimer. The AUC value of the ROC curve of MPV, PDW and D-dimer in predicting severity was 79% (p=0.001, 95% CI: 0.696-0.885), 72.5% (p&lt;0.001, 95% CI: 0.598-0.852), and 97% (p&lt;0.001, 95% CI: 0.937-1.00).Conclusions: This study found a relationship between MPV and PDW values on the severity of COVID-19. There’s no relationship of MPV and PDW to D-dimer concentration.

Association between mean platelet volume and pulmonary embolism: a systematic review and meta-analysis.

Platelet activation plays an important role in the progression of pulmonary embolism (PE). Mean platelet volume (MPV) can serve as a marker of platelet activity in patients with PE. Many studies have reported different results regarding the relationship. Therefore, we aimed to perform a systematic review and meta-analysis to evaluate the relationship between MPV and PE. Two reviewers independently searched relevant articles in databases from inception to April 21, 2021 and identified all studies on MPV and PE as the outcomes of interest. Further, we selected studies meeting the criteria and extracted the data. Of the 2505 publications identified, we included 18 studies after screening. Results showed MPV was significantly higher in patients with PE (0.83 fL, 95% CI: 0.38-1.28, P<0.001) than in controls. The mean difference in MPV between those who died and survivors of PE was 1.23 fL (95% CI: 0.96-1.51, P<0.001). Hence, an increased MPV is associated with PE. MPV could be a useful tool to predict the occurrence and death risk of PE together with other risk factors.

THE DIFFERENCE OF MEAN PLATELET VOLUME AND PLATELET COUNT IN CORONARY HEART DISEASE PATIENTS WITH ACUTE CORONARY SYNDROME AND NON ACUTE CORONARY SYNDROME

Background: Coronary Heart Disease (CHD) is currently one of main causes and the first death in developed and developing countries, including Indonesia. CHD appeared with the clinical appearance of asymptomatic, stable angina pectoris until acute coronary syndrome. ACS is a progressively CHD and takes parameters that describe the process pathogenesis. MPV and platelet count are two parameters in the complete blood count which reflect the activity of platelets in CHD.Objective: This study is to investigate the differences Mean Platelet Volume and Platelet Count in CHD with ACS compared to non ACS.Methods: A cross-sectional study was conducted with collecting data from the medical records of patients with CHD who underwent treatment in the dr. Kariadi General Hospital Medical Center Semarang, the period of January - December 2019. Eighty subjects of 20 non-ACS patients and 60 ACS patients were included in this study which is Mean Platelet Volume and Platelet Count in hematology laboratory were analyzed. The Independent T-test were used for comparing the group means.Results: MPV was found to be higher significantly among ACS patients as compared to non ACS, 9,43 ± 0,3868 fl vs 9,43 ± 0,3868 fl (p-value&lt;0.01) while Platelet Count was lower significantly among ACS patients as compared to non ACS, 282,63 ± 75,084 109/L vs 322,30 ± 41,980 109/L.Conclusion: There were significantly differences in Mean Platelet Volume and Platelet count between patients with ACS and non ACS.