Differences In Diatom Assemblages Research Articles

Biogenic silica accumulation and diatom assemblage variations through the Eocene-Oligocene Transition: A Southern Indian Ocean versus South Atlantic perspective

It is widely interpreted that there is a significant link between climate cooling at the Eocene-Oligocene Transition (EOT; ∼34 Ma) and subsequent diatom proliferation in the world's oceans during the mid to late Cenozoic. Yet, our understanding of biogenic silica flux through the EOT interval is based on limited data from a few sites, and there are many complications in making a meaningful comparison based on biogenic silica concentration data generated using different techniques. Here, we present new biogenic opal flux and diatom assemblage records across EOT from Ocean Drilling Program Site 748 (Southern Kerguelen Plateau, southern Indian Ocean), in addition to new biogenic opal flux records from the South Atlantic (Deep Sea Drilling Project Site 511, Falkland Plateau and Ocean Drilling Program Site 1090, Agulhas Ridge). Observed opal flux shifts and variability at Site 748 are consistent with published data from nearby Site 744; both sites show considerable shifts in biogenic opal accumulation rates corresponding to shifts in published benthic oxygen isotope records through the EOT. In contrast, our new opal flux record for Site 511, derived from biogenic opal concentration measurements via spectrophotometry, differs from the published record derived from insoluble residues, whereas new data generated for Site 1090 are generally consistent with previously published flux reconstructions. The South Atlantic biogenic opal flux records, however, are dissimilar from one another, and both are dissimilar from the Southern Indian Ocean records. Also, the taxonomic composition of the diatom assemblages from Sites 511, 748 and 1090 display considerable differences, with hemiauloids and rhizosolenids – generally inferred as indicative of oligotrophic conditions – being the dominant diatoms at the Southern Kerguelen Plateau (Site 748). Published records show that hemiauloid taxa are absent from the earliest Oligocene interval at Site 1090, which is widely seen as a record of deposition from nutrient-rich waters sustaining abundant diatom assemblages. These differences in diatom assemblages testify to regional differences in nutrient concentrations. In particular, the timing of biogenic opal flux pulses between Sites 1090 and 748 imply a shift in the locus of opal deposition to areas further south in the Southern Ocean across the EOT, likely related to proto-ACC development and strengthening of frontal boundaries. Thus, combined geochemical and micropaleontological evidence points to a regionally variable rather than a global, unified opal flux response to climate cooling through the Eocene-Oligocene Transition.

Long-term response of diatoms in high-elevation streams influenced by rock glaciers

In this study, we monitored diatom assemblages at various sites along a stream-lake network draining a glaciated alpine cirque landscape (2600 m asl) in the Swiss National Park over 19 years (2001–2020). The primary goal of this study was to examine the long-term changes in diatom assemblages in the running waters at high-elevation influenced by rock glaciers, and particularly the use of diatoms as indicators of environmental change. Because streams in the catchment are interconnected with lakes, another objective was to compare diatom assemblages between lake inlets and lake outlets as well as along the chain of lakes. The Macun cirque comprises two main drainage basins with one influenced by waters from rock glaciers. Lakes are interconnected by stream segments in both basins before merging at an outflow lake. We expected changes in diatom assemblages would reflect changes in long-term physico-chemistry of surface waters between basins, between lake inlets and outlets, and along lake chains. The novelty of this work is that covers a unique heterogeneous aquatic environment in the Alps affected by climate change, an area where the temperature has increased over twofold more than other regions in the northern hemisphere. This long-term study provides the opportunity of forecasting how other alpine freshwater ecosystems globally may change in the future. We combined a classical taxonomic approach and scanning electron microscopy (SEM) along with physico-chemical data across a broad spatio-temporal scale. Diatom assemblages showed distinct spatial differences between basins, reflecting basin-wide differences in water physico-chemistry. Diatoms in both basins displayed a major temporal shift in composition between samples collected pre-2010 with those collected post-2010 that also followed temporal changes in physico-chemistry, suggesting a tipping point occurred in the system. There were subtle differences in diatom assemblages between lake inlets and outlets, most evident in the precipitation/groundwater fed north basin. Lastly, a clear lake order effect was detected in both basins with upper basin sites differing from lower basin sites at similar altitude. We conclude that diatoms were effective in documenting the environmental changes that occurred in these alpine waters, specifically reflecting the abrupt change in physico-chemistry (concurrent tipping point in diatom assemblages) and the concomitant increase in surface water homogeneity and similarity of diatom assemblages over the study period.

Morphotaxonomy- and metabarcoding-based ecological assessment of Cyprus streams’ diatom communities and correlation with environmental and anthropogenic influences

In freshwater ecosystems, periphytic biofilms include diatom assemblages that depend on environmental conditions (e.g., nutrient concentrations, salinity, temperature etc.). These assemblages respond rapidly to environmental changes, which makes diatoms valuable bioindicators. For this reason, they are currently used in freshwater biomonitoring programs (e.g., EU Water Framework Directive - WFD) (Foster et al., 2000). To date, diatom taxonomic identification is based on morphological criteria, which requires high taxonomic expertise to identify them to the species level needed for biomonitoring. Having this in mind, new strategies have been examined for the development of high-throughput, non-biased identification approaches. Human activities are the leading cause of environmental impairments and appropriate biomonitoring of ecosystems is needed to effectively assess the impact of their activities. In the last ten years, DNA metabarcoding combined with next-generation sequencing and bioinformatics, have been proposed as a complementary approach to morphological identification. In the past ten years, DNA metabarcoding coupled with next-generation sequencing and bioinformatics represents a complementary approach for diatom biomonitoring (Vasselon et al., 2019). In this study, this approach was used for the first time in Cyprus considering the association of environmental and anthropogenic pressures to diatom assemblages using the rbcL 312 bp barcode, next-generation sequencing (MiSeq Illumina), and bioinformatic evaluation (Mothur Software). Statistical analysis was then applied to identify the environmental (i.e., river types, geo-morphological) and anthropogenic (i.e., physical, chemical, human land-use pressures) variables' role in the observed diatom diversity. The Indice de Polluosensibilité Spécifique (IPS) index was used as it was shown to better respond to pressures that affect water quality in Cyprus rivers (WDD, 2014). Results indicate differences in diatom assemblages between intermittent and perennial rivers. Achnanthidium minutissimum was more abundant in intermittent rivers; whereas Amphora pediculus and Planothidium victorii (P. caputium) in perennial ones. Furthermore, we could demonstrate the correlation between nutrients (e.g., nitrogen, phosphorus), characteristics of the individual sampling sites (e.g., elevation), and land use activities on the observed differences in diatom diversity (Pissaridou, 2021). Additionally, results were compared to the morphotaxonomy-based approach which was conducted microscopically. Results show a positive correlation between morphological and molecular IPS scores. Points deviating from the norm are influenced by the limitations of both techniques. Fistulifera saprophila had a key role in this observation, as it negatively influences IPS scores. All in all, we conclude that DNA metabarcoding complements the morphological methodology for the ecological quality assessment of freshwaters in Cyprus. Multi-stressors and anthropogenic pressures have a significant statistical relationship to the observed diatom diversity and play a pivotal role in determining Cyprus' rivers' ecological status (Fig. 1). Foster, D., Wood, A., Griffiths, M., 2000. The Water Framework Directive (2000/60/EC) – An introduction Dave Foster – Policy Advisor (Europe), Aram Wood EP Scientist (Water), Dr Martin Griffiths – Head of Water Quality, Environment Agency, Head Office, Rio House, Waterside Drive, Aztec West, Almon 7–9. Pissaridou, P., Vasselon V., Christou A., Chonova T., Lacroix S., Papatheodoulou A., Drakou K., Tziortzis I., Dörflinger G., Rimet F., Bouchez A. and Vasquez MI. 2021 Deciphering Cyprus’ diatom diversity and the effects of environmental and anthropogenic influences for ecological assessment of rivers using DNA metabarcoding.Chemosphere (In Press) Vasselon, V., Frédéric, R., Isabelle, D., Olivier, M., Yorick, R., Agnès, B., 2019. Assessing pollution of aquatic environments with diatoms’ DNA metabarcoding: Experience and developments from France Water Framework Directive networks. Metabarcoding and Metagenomics 3, 101–115. https://doi.org/10.3897/mbmg.3.39646 WDD, 2014. Review and update of article 5 of Directive 2000/60/EC (Water reservoirs) & Classification of water status (Rivers, natural lakes and water reservoirs), That will establish baseline information and data for the 2nd cyprus river basin management plan.

Cyprus’ diatom diversity and the association of environmental and anthropogenic influences for ecological assessment of rivers using DNA metabarcoding

Human activities are the leading cause of environmental impairments. Appropriate biomonitoring of ecosystems is needed to assess these activities effectively. In freshwater ecosystems, periphytic and epilithic biofilms have diatom assemblages. These assemblages respond rapidly to environmental changes, making diatoms valuable bioindicators. For this reason, freshwater biomonitoring programs are currently using diatoms (e.g., Water Framework Directive). In the past ten years, DNA metabarcoding coupled with next-generation sequencing and bioinformatics represents a complementary approach for diatom biomonitoring. In this study, this approach is used for the first time in Cyprus by considering the association of environmental and anthropogenic pressures to diatom assemblages. Statistical analysis was then applied to identify the environmental (i.e., river types, geo-morphological) and anthropogenic (i.e., physicochemical, human land-use pressures) variables’ role in the observed diatom diversity. Results indicate differences in diatom assemblages between intermittent and perennial rivers. Achnanthidium minutissimum was more abundant in intermittent rivers; whereas Amphora pediculus and Planothidium caputium in perennial ones. Additionally, we could demonstrate the correlation between nutrients (e.g., nitrogen, phosphorus), stations’ local characteristics (e.g., elevation), and land use activities on the observed differences in diatom diversity. Finally, we conclude that multi-stressors and anthropogenic pressures together as multiple stressors have a significant statistical relationship to the observed diatom diversity and play a pivotal role in determining Cyprus’ rivers’ ecological status.

Factors affecting diatom distribution in floodplain lakes of the southeast Murray Basin, Australia and implications for palaeolimnological studies

Diatom assemblages of surface sediments in 46 billabongs from four river floodplains in the southeast Murray-Darling Basin, Australia were sampled to investigate drivers of species distribution. The principal purpose of the study was to derive information to aid interpretation of diatom-based palaeoecological studies of these systems and of floodplain lakes more generally. Patterns in billabong diatom assemblages in relation to river reach, hydrology and farming intensity on surrounding land were examined, as were correlations with water quality variables. Seasonal variation in billabong water quality was high relative to spatial variation, and spatial patterns in billabong water quality were weak. In contrast, strong patterns were evident in diatom assemblages. Three main patterns were observed: (1) a distinction between billabongs dominated by planktonic diatoms from those dominated by benthic and attached forms; (2) differences in diatom assemblages in billabongs on different river reaches; and (3) differences in assemblages in billabongs with different hydrology. Of all water quality variables tested, total phosphorus (TP), total nitrogen (TN) and pH exerted the strongest independent influence on diatom distribution; however, only TP remained an important variable when species variation due to river reach, hydrology, and aquatic plant cover was taken into account. The weak influence of water quality on diatom distribution is interpreted as reflecting the dichotomy between plankton and non-plankton-dominated billabongs, the influence of hydrology and biogeography, the lack of strong spatial water quality gradients and the high degree of temporal variability in water quality. The findings show that diatom records from billabong sediments can provide evidence of long-term changes in the abundance of aquatic macrophytes and hydrology. They also suggest that merging calibration data sets across regions for the purpose of improving diatom transfer functions for water quality reconstruction is of limited value for floodplain lakes, and that performance is more likely to be gained by boosting site numbers within regions.

Comparison of contemporary and fossil diatom assemblages from the western Antarctic Peninsula shelf

During the past ten years, the Antarctic Peninsula has been identified as the most rapidly warming region of the Southern Hemisphere and it is important to place this warming in the context of the natural climate and oceanographic variability of the recent geological past. Many biological proxies, such as marine diatom assemblages, have been used to determine Southern Ocean palaeoceanographic conditions during the Late Quaternary, however, few investigations have attempted to link observations of modern floras with the fossil record. In this study we examine a suite of modern austral spring (December 2003) and summer (February 2002) surface water samples from along the western Antarctic Peninsula (WAP) continental shelf and compare these to core-top, surface sediment samples. Using detrended correspondence analysis (DCA) and principal component analysis (PCA) of diatom abundance data we investigate the relationship of contemporary diatom floras with the fossil record. This multivariate analysis reveals that our modern assemblages can be divided into three groups: summer southern WAP sites, summer northern WAP sites, and spring WAP sites. Sea surface temperature (SST) is an important environmental variable for explaining seasonal differences in diatom assemblages between spring and summer, but sea surface salinity (SSS) is more important for understanding temporally-equivalent regional variations in assemblage. Our summer diatom samples are more reminiscent of early season assemblages, reflecting the unusually late sea ice retreat from the region that year. When the modern assemblages are compared to the fossil record, it is clear that most of the important diatoms from the summer assemblage are not preserved into the sediments, and that the fossil record more closely reflects spring assemblages. This observation is important for any future attempts to quantitatively reconstruct palaeoceanographic conditions along the WAP during the Late Quaternary and highlights the need for many more such studies in order to address longer timescales, such as interannual variability, in the context of the fossil record.

Distribution of diatom assemblages and their relationship to environmental variables in the surface sediments of three northern Egyptian lakes

Surficial sediments of three northern Egyptian lakes (Manzala, Burullus and Edku) show differences in diatom assemblages deposited in different sites of these lakes. A total of 172 species and varieties belonging to 58 genera were identified and counted from 62 samples. Of these, 163 diatom taxa were recorded from Manzala Lake sediments, 147 taxa were found in Burullus Lake sediments, and 117 taxa were identified in Edku Lake sediments. The considerable variation in the composition and distribution of the diatom assemblages among these lakes was mainly related to differences in the water quality, salinity, the concentration of nutrients and climatic changes. The planktonic diatom Cyclotella meneghiniana was dominant in the majority of the samples from Manzala Lake, but dominant in only a few samples from the middle parts of Burullus and Edku lakes. The non-planktonic epiphytic taxa Cocconeis placentula and Epithemia sorex were the subdominant species in the surface sediments, especially in shallow and marginal parts of the lakes. Multivariate statistical techniques (hierarchical ascending clustering and canonical correspondence analysis) were used to identify ecological groups of diatoms and to investigate which environmental variables were important in explaining the variation between these groups. Eight ecological groups containing distinctive diatom assemblages reflect current environmental conditions; especially saltwater intrusion in the north and nutrient-rich freshwater in the south.

PERIPHYTIC DIATOM ASSEMBLAGES FROM ULTRA‐OLIGOTROPHIC AND UV TRANSPARENT LAKES AND PONDS ON VICTORIA ISLAND AND COMPARISONS WITH OTHER DIATOM SURVEYS IN THE CANADIAN ARCTIC1

Periphytic diatoms are potentially powerful indicators of environmental change in climatically‐sensitive high latitude regions. However, only a few studies have examined their taxonomic and ecological characteristics. We identified and enumerated diatom assemblages from sediment, rock, and moss habitats in 34 ultra‐oligotrophic and highly transparent lakes and ponds on Victoria Island, Arctic Canada. The similar limnological characteristics of the sites allowed us to examine the influence of habitat, independent of water chemistry, on the diatom assemblages. As is typical in shallow arctic water bodies, benthic taxa, including species of Achnanthes, Caloneis, Cymbella, Navicula, and Nitzschia, were most widely represented. Minor gradients in our measured environmental variables did not significantly explain any variance in diatom species, but there were marked differences in diatom assemblages among sites. Pond ephemerality seems to explain some diatom variation, because aerophilic taxa such as Achnanthes kryophila Petersen and A. marginulata Grunow were dominant in shallow sites that had undergone appreciable reductions in volume. We identified several taxa that exhibited strong habitat preferences to sediment, moss, or rock substrates and also found significant differences (P < 0.01) in diatom composition among the three habitats. In comparisons with three similar diatom surveys extending over 1200 km of latitude, we determined that surface sediment assemblages differed significantly (P < 0.001) among all regions examined. Diatom species diversity was inversely related to latitude, a result likely explained by differences in the lengths of growing seasons. These data contribute important ecological information on diatom assemblages in arctic regions and will aid in the interpretation of environmental changes in biomonitoring and paleolimnological studies.

Ecoregions and benthic diatom assemblages in Mid-Atlantic Highlands streams, USA

AbstractEcoregional differences in geology and hydrology may affect physical and chemical conditions in streams and, consequently, the species composition of algal assemblages. Stresses resulting from human disturbance, however, may constrain species membership in algal assemblages and reduce regional diversity. We expected that ecoregional differences in diatom assemblages, if they were present, would be more evident in relatively undisturbed sites than in randomly selected sites. Benthic diatom and water chemistry samples were collected from streams in 7 ecoregions of the Mid-Atlantic Highlands to evaluate correspondence between ecoregional classification and diatom assemblages. Ecoregional differences were assessed using 196 randomly selected stream sites (probability sites) and 60 sites with less disturbance by humans (reference sites). Multivariate analyses showed that significant ecoregional differences in diatom assemblages were observed only in probability sites and not in reference sites. Water c...

Factors influencing the distribution of diatoms and other algae in the Ross Sea

Quantitative microscopic analyses of sediment trap samples collected in the Ross Sea between January 1990 and February 1992 reveal striking temporal and spatial differences in algal bloom composition and size. Trap samples from the southwestern Ross Sea (site A, 76°30′S, 167°30′E) were dominated by the diatom Fragilariopsis curia (maximum of 92%), a species associated with both sea ice and the retreating ice edge in the Ross Sea. This species was probably seeded by melting congelation ice. Highest flux of diatom valves to both upper and lower traps in the 1991–1992 season occurred as a distinct event in mid‐February 1991, after which flux decreased by 1 to 3 orders of magnitude. As overall flux decreased, an increase in relative abundance of Chaetoceros resting spores and the open water species Thalassiosira antarctica was observed in the lower trap, suggesting some localized lateral advection. In the south central Ross Sea (site B, 76°30′, S 175°W), algal diversity was much higher, with a greater contribution of nondiatom material. The prymnesiophyte Phaeocystis antarctica, dinoflagellates, and cysts of unknown affinity were much more common than at site A, as were the diatoms Fragilariopsis cylindrus, Fragilariopsis kerguelensis, and Thalassiosira gracilis. Highest diatom flux occurred later (February 20 to March 6,1991) at this site and was an order of magnitude lower as compared to maximum flux at site A. Distinct differences in bloom size and composition between sites A and B may be a function of upper water column structure, differences in the amount of sea ice melting, and the type of sea ice present at the time of ice breakout (congelation versus pack ice). Despite rich surface productivities, lower silica flux to the seafloor at site B results from the higher proportion of nonsiliceous algae. At site C (72°30′S, 172°30′E), in the northwestern Ross Sea, diatoms again dominated algal flux; however, assemblage composition differed from that observed at both sites A and B. Relative abundance of F. curta was lower, averaging 60–70%, while the remainder of the assemblage was made up of F. cylindrus, other more robust species of Fragilariopsis, and Chaetoceros resting spores. Algal flux at site C is intermediate in style between sites A and B. Significantly, spatial differences in diatom assemblages noted above appear to be reflected in seafloor surface sediments, suggesting that downcore diatom data provide an interpretable record of paleoproductivity in the Ross Sea.

CHANGES IN THE PLANKTONIC DIATOM FLORA OF A LARGE MOUNTAIN LAKE IN RESPONSE TO FERTILIZATION1

ABSTRACTFertilization (phosphorus and nitrogen) of Kootenay Lake, British Columbia, was begun in 1992 in an attempt to increase food resources for declining stocks of kokanee salmon. In 1992 and 1993, significant changes in diatom abundance, species composition, diversity, and morphology of individual species were observed in the fertilized North Arm in comparison to the unfertilized South Arm. Effects of fertilization were most obvious during the spring. During summer stratification, fertilization did not result in significant changes in total abundance, but changes in species composition were observed. In 1993, with continued fertilization of the North Arm, diatom abundance in both spring and summer was significantly higher than at the same sites in 1992. No significant differences were observed in the unfertilized South Arm. Diatom abundance was significantly correlated with dissolved inorganic nitrogen (positive) and dissolved reactive silica (negative). Diatom assemblages in the fertilized North Arm in both the spring and summer of 1993 were dominated by Asterionella formosa and Fragilaria crotonensis, taxa considered mesotrophic. Abundance of the oligotrophic taxa Rhizosolenia eriensis and Cyclotella michiganiana was higher in the South Arm than in the North Arm. Differences in diatom assemblages between the two arms in both years were further summarized by multivariate statistical analyses. Morphological variations in two small Cyclotella species (C. comensis and C. cf. pseudostelligera) were observed between the fertilized and “control” stations. Populations of both species from fertilized stations exhibited stronger silicification with coarser striations and a smaller mean size.